Academic literature on the topic 'FISH (Fluorescence In Situ Hybridisation)'

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Journal articles on the topic "FISH (Fluorescence In Situ Hybridisation)"

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Edwards, A. A. "Editorial - Fluorescence In Situ Hybridisation (FISH)." Radiation Protection Dosimetry 88, no. 1 (March 1, 2000): 5–6. http://dx.doi.org/10.1093/oxfordjournals.rpd.a033019.

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Mudhar, Hardeep Singh, Kath Smith, Polly Talley, Abigail Whitworth, Neil Atkey, and Ian G. Rennie. "Fluorescence in situ hybridisation (FISH) in histologically challenging conjunctival melanocytic lesions." British Journal of Ophthalmology 97, no. 1 (November 8, 2012): 40–46. http://dx.doi.org/10.1136/bjophthalmol-2012-302261.

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Beverstockl, Geoffrey C., Martine de Jager, Ite de Waard-Siebinga, Jeanette Kool, Paul Mollevanger, Hans Wessels, and Ed Hoogendoorn. "Cytogenetic and fluorescence in situ hybridisation (FISH) analysis of ocular melanoma." Cancer Genetics and Cytogenetics 77, no. 2 (October 1994): 182. http://dx.doi.org/10.1016/0165-4608(94)90365-4.

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Harrison, R. H., H. C. Kuo, P. N. Scriven, A. H. Handyside, and C. Mackie Ogilvie. "Lack of cell cycle checkpoints in human cleavage stage embryos revealed by a clonal pattern of chromosomal mosaicism analysed by sequential multicolour FISH." Zygote 8, no. 3 (August 2000): 217–24. http://dx.doi.org/10.1017/s0967199400001015.

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Multicolour fluorescence in situ hybridisation (FISH) analysis of interphase nuclei in cleavage stage human embryos has highlighted a high incidence of postzygotic chromosomal mosaicism, including both aneuploid and ploidy mosaicism. Indeed, some embryos appear to have a chaotic chromosomal complement in a majority of nuclei, suggesting that cell cycle checkpoints may not operate in early cleavage. Most of these studies, however, have only analysed a limited number of chromosomes (3–5), making it difficult to distinguish FISH artefacts from true aneuploidy. We now report analysis of 11 chromosomes in five sequential hybridisations with standard combinations of two or three probes and minimal loss of hybridisation efficiency. Analysis of a series of arrested human embryos revealed a generally consistent pattern of hybridisation on which was superimposed frequent deletion of one or both chromosomes of a specific pair in two or more nuclei indicating a clonal origin and continued cleavage following chromosome loss. With a binucleate cell in a predominantly triploid XXX embryo, the two nuclei remained attached during preparation and the chaotic diploid/triphoid status of every chromosome analysed was the same for each nucleus. Furthermore, in each hybridisation the signals were distributed as a mirror-image about the plane of attachment, indicating premature decondensation during anaphase consistent with a lack of checkpoint control.
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NIEUWINT, A. W. M. "Rapid detection of microdeletions using fluorescence in situ hybridisation (FISH) on buccal smears." Journal of Medical Genetics 37, no. 6 (June 1, 2000): 4e—4. http://dx.doi.org/10.1136/jmg.37.6.e4.

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Baldovini, Chiara, Anna L. Tosi, Enrico Di Oto, Camilla Reggiani, Susanna Cappia, Christine M. Betts, Carmine Gallo, Lisa Ricchieri, Roberto Cocchi, and Maria P. Foschini. "Genetic markers of oral malignant melanoma analysed by fluorescence in situ hybridisation (FISH)." Virchows Archiv 459, no. 2 (June 29, 2011): 167–73. http://dx.doi.org/10.1007/s00428-011-1107-9.

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Vuoriranta, P., M. Männistö, and H. Soranummi. "Occurrence of Sphingomonas sp. bacteria in cold climate drinking water supply system biofilms." Water Supply 3, no. 1-2 (March 1, 2003): 227–32. http://dx.doi.org/10.2166/ws.2003.0108.

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Members of the bacterial genus Sphingomonas (recently split into four genera), belonging to α-4-subclass of Proteobacteria, were isolated and characterised from water distribution network biofilms. Water temperature in the studied network, serving 200,000 people, is less than 5°C for about five months every winter. Sphingomonads, characterised using fluorescent oligonucleotide probes and fatty acid composition analysis (FAME), were a major group of bacteria among the distribution network biofilm isolates. Intact biofilms, grown on steel slides in a biofilm reactor fed with tap water, were detected in situ using fluorescence labelled oligonucleotide probes (FISH). Hybridisation with probes targeted on α-proteobacteria and sphingomonads was detected, but FISH on intact biofilms suffered from non-specific hybridisation and intensive autofluorescence, possibly due to extracellular material around the bacterial cells attached to biofilm. These preliminary results indicate that bacteria present in the distribution network biofilms in this study phylogenetically differ from those detected in more temperate regions.
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McNeil, Nicole, and Thomas Ried. "The role of cytokines in immunological tolerance: potential for therapy." Expert Reviews in Molecular Medicine 2, no. 7 (September 14, 2000): 1–14. http://dx.doi.org/10.1017/s1462399400001940.

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Molecular cytogenetic techniques that are based on fluorescence in situ hybridisation (FISH) have become invaluable tools for the diagnosis and identification of the numerous chromosomal aberrations that are associated with neoplastic disease, including both haematological malignancies and solid tumours. FISH can be used to identify chromosomal rearrangements, by detecting specific DNA sequences with fluorescently labelled DNA probes. The technique of comparative genomic hybridisation (CGH) involves two-colour FISH. It can be used to establish ratios of fluorescence intensity values between tumour DNA and control DNA along normal reference metaphase chromosomes, and thereby to detect DNA copy-number changes such as gains and losses of specific chromosomal regions and gene amplifications. Spectral karyotyping (SKY) is a novel molecular cytogenetic method for characterising numerical and structural chromosomal aberrations. SKY involves the simultaneous hybridisation of 24 differentially labelled chromosome-painting probes, followed by spectral imaging and chromosome classification, and produces a colour karyotype of the entire genome. The use of SKY has contributed significantly to the identification of chromosomal anomalies that are associated with constitutional and cancer cytogenetics, and has revealed many aberrations that go undetected by traditional banding techniques. In this article, we have reviewed these new molecular cytogenetic techniques and described their various applications in molecular medicine.
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Chatzimeletiou, Katerina, George Makrydimas, Alexandros Sotiriadis, Evagelos Paraskevaidis, and Kypros H. Nicolaides. "Aneuploidy screening in coelomic samples using fluorescencein situ hybridisation (FISH)." Prenatal Diagnosis 25, no. 10 (2005): 919–26. http://dx.doi.org/10.1002/pd.1227.

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Tafe, Laura J., Heather B. Steinmetz, Samantha F. Allen, Betty J. Dokus, and Gregory J. Tsongalis. "Rapid fluorescence in situ hybridisation (FISH) for HER2 (ERBB2) assessment in breast and gastro-oesophageal cancer." Journal of Clinical Pathology 68, no. 4 (January 9, 2015): 306–8. http://dx.doi.org/10.1136/jclinpath-2014-202787.

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Evaluation of HER2 (ERBB2) gene amplification or protein expression is standard of care in breast (BR) and advanced stage gastro-oesophageal cancers to identify patients eligible for anti-HER2 therapies. Here, we evaluate a rapid fluorescence in situ hybridisation (FISH) technology (HER2 instant quality (IQ) FISH pharmDx Kit) for detection of HER2 in patients with BR and gastro-oesophageal cancer using 30 FFPE samples that had been previously evaluated with the PathVysion HER2 DNA Probe Kit. Cases were scored as positive (HER2:CEN-17 ≥2.0), negative (HER2:CEN-17 <2.0) or equivocal according to the ASCO/CAP 2013 BR cancer guidelines. Ten samples were positive for HER2 amplification while 20 were negative; none were equivocal. The IQ FISH was able to detect low level amplification (HER2:CEN-17 ratio 2.4). The HER2 IQ FISH pharmDx Kit is a FDA approved kit that offers a rapid turnaround time (approximately 3.5 h) and in our laboratory was 100% concordant with prior PathVysion results.
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Dissertations / Theses on the topic "FISH (Fluorescence In Situ Hybridisation)"

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HajMohammadi, Sassan. "Development of FISH technology in pathological tissue." Thesis, University of Southampton, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.284578.

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Williams, Lisa. "Fluorescence in situ hybridisation (FISH) analysis of chromosomal aberrations in gastric tissue : the involvement of Helicobacter pylori." Thesis, Swansea University, 2004. https://cronfa.swan.ac.uk/Record/cronfa43067.

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Gastric cancer is a common cause of cancer death in the UK. It most often presents in patients when the disease is advanced, and hence treatment options are limited. As such, studies on the pre-malignant stages of gastric cancer, and interest in the mechanisms of the carcinogenic process (reactive oxygen species, ROS) and the agents that may drive the carcinogenic pathway {Helicobacter pylori infection), are important, with a view to improving disease outcome. This series of experiments has firstly shown, using CBMN assay +/- kinetochore staining and interphase FISH, that ROS causes aneuploidy of chromosomes 4, 8, 20 and 17(p53) in a human cell line. Secondly, gastric cells have been collected using endoscopic cytology brush techniques, and prepared, such that interphase FISH could be performed. Again, aneuploidy of chromosomes 4, 8, 20 and 17(p53) were detected in normal gastric mucosa, gastritis and intestinal metaplasia. The level of aneuploidy detected increased as disease severity increased. Amplification of chromosome 4, amplification of chromosome 20 and deletion of chromosome 17(p53) were the more significant findings. The degree of chromosomal abnormalities detected increased further, in a stepwise manner, when gastric dysplasia and gastric adenocarinoma cells were studied. Hence, a role for these abnormalities may exist in the initiation of, and the progression to, gastric cancer. The presence of H. pylori was also determined in the gastric tissue studied using histological analysis and PCR technology. Detection rates were comparable. The more virulent strain of H. pylori, Cag A, was found to be associated with increased disease pathology and chromosomal abnormalities, yet numbers were small. The amplification of chromosome 4 in gastric tissue was again highlighted in association with H pylori infection, hence it may reflect a role for chromosome 4 in the initiation of gastric cancer.
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Leversha, Margaret Anne. "Cytological estimations of molecular genetic difference : applications and implications of fluorescence in situ hybridisation mapping in the long arm of human chromosome 9." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.337902.

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Stindl, Martin Maria Matthias. "Evaluation of ligation methods and the synthesis of a specific PNA-encoded peptide library." Thesis, University of Edinburgh, 2015. http://hdl.handle.net/1842/17927.

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Dysfunctional or over and under expressed enzymes play a crucial role in a variety of diseases. A tool that can identify dis-regulated enzymes in individual patients would be beneficial and would allow personalised treatment. For this purpose, a 10,000 membered ‘spit-and-mix’ PNA-encoded peptide library with a cell penetrating peptide was synthesised and interrogated with K562 cell lysate and intact K562 cells. This allowed the specific enzyme activity pattern for ABL tyrosine kinase from both inside a cell and a lysate to be obtained. Hybridisation of this library with a DNA-microarray resulted in bio-fouling by the cell lysate, thereby preventing analysis of the phosphorylation pattern. To allow extraction and purification of the peptide library from the cell lysates, a His-tag was incorporated into the library, and enabled successful library analysis. In addition to this 10,000 member library, a focused 100 PNA-encoded peptide library was synthesised. The library included peptide sequences known to be phosphorylated by specific tyrosine kinases deregulated in acute lymphoblastic leukaemia (ALL) with a PNA-tag complementary to a DNA microarray. Different ligation methods to conjugate the peptides to PNA-tags were screened – this included amide coupling, copper catalysed azide–alkyne cycloaddition, strain promoted azide–alkyne cycloaddition and Diels–Alder cycloaddition. The inverse electron demand Diels–Alder cycloaddition between a tetrazine and norbornene was chosen as the preferred ligation method, and the reaction conditions optimised. To purify the library from cell lysate, a His-tag was again coupled to each member using the strain promoted azide–alkyne cycloaddition. To test the tetrazine ligation, fluorescence in situ hybridisation (FISH) was used in cells, whereby a fluorophore was ligated onto a tetrazine–conjugated PNA probe. This was hybridised onto an mRNA in fixed cells. Results indicated that the ligation needed further optimisation.
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Somasekar, Amudha. "Fluorescence in situ hybridisation (FISH) analysis of chromosome 1 and gene expression levels of MAD2 and BUB1 levels in premalignant stages of gastric tissue." Thesis, Swansea University, 2011. https://cronfa.swan.ac.uk/Record/cronfa43141.

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Gastric cancer is a common cause of cancer death in the world. The mortality rate from gastric cancer is high in UK as it often presents late, often with local or distant metastasis. This makes the treatment options limited. The pathogenesis of gastric cancer occurs in a multi step pathway with pre cancerous conditions leading to cancer eventually. It is important to understand this carcinogenic process (aneuploidy and abnormal gene expression levels) and the driving forces (eg. Helicobacter pylori infection) which will enable us to alter the disease outcome. This series of experiments included cytogenetic investigation which involved obtaining gastric cells using brush cytology and using Fluorescent in situ hybridisation technique to look for aneuploidy levels of chromosome 1 and 4. These two chromosomes were chosen as chromosome 1 has been recently shown to be abnormal in early in premalignant stages of gastric cancer. Chromosome 4 was chosen as hyperploidy of chromosome 4 was the predominant chromosomal aberration in Barrett’s oesophagus. This study has shown that the aneuploidy level of chromosome 1 progressively increased with the progression of the histological stages according to the Correa’s premalignant gastric cancer pathway. Significant increase in aneuploidy levels of chromosome 1 was seen in H. Pylori associated gastritis, implying that H. Pylori play a very important role in the progression of the disease. Aneuploidy can occur due to various genetic defects that may potentially occur during mitosis. Spindle cell check points play a vital role in preventing the cells from proceeding to the anaphase stage if there is any defect in the kinetochore attachment. Certain genes like MAD2 and BUB1 are thought to be instrumental in controlling the spindle cell check points and it is believed a steady state of genes like MAD2 and BUB1 are required for this. In the second part of this study, the MAD2 and BUB1 expression levels were measured and correlated to the aneuploidy stages. There was no significant difference in their expression levels in patients with significant aneuploidy level. MAD2 levels were increased in H.Pyloriassociated gastritis, which implies that H. Pylori plays an important role in the pathogenesis of gastric cancer.
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Varela, Filipa Alexandra Pereira Rosa. "Tipificação de Mycoplasma mycoides subsp. mycoides SC e detecção da sua aderência a células epiteliais pulmonares." Master's thesis, Faculdade de Ciências e Tecnologia, 2010. http://hdl.handle.net/10362/5092.

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Dissertação apresentada para a obtenção do Grau de Mestre em Genética Molecular e Biomedicina, pela Universidade Nova de Lisboa, Faculdade de Ciências e Tecnologia
A Peripneumonia Contagiosa Bovina (PPCB) é uma doença respiratória infecciosa, de grande relevo no âmbito da produção de gado bovino, causada pela bactéria Mycoplasma mycoides subsp. mycoides SC (MmmSC). Actualmente, a PPCB permanece endémica apenas em África, sendo aí responsável por perdas incontornáveis no sector pecuário. O último surto de PPCB detectado na Europa ocorreu em Portugal em 1999 mas, devido à sua natureza insidiosa, o risco de re-emergência é permanente. As estirpes de MmmSC associadas aos últimos surtos europeus de PPCB são tradicionalmente consideradas geneticamente muito homogéneas. No entanto, trabalhos recentes de tipificação molecular revelaram a existência de uma variabilidade genética intraspecífica superior ao que se esperava. A realização deste trabalho teve como base dois objectivos fundamentais: a tipificação de estirpes de MmmSC isoladas durante os últimos surtos de PPCB que ocorreram em Portugal entre 1993 e 1998, e a aplicação de um método alternativo e inovador para a detecção e quantificação de MmmSC, após a infecção de culturas celulares. Para a concretização do primeiro objectivo foi realizada uma análise da variabilidade polimórfica de três regiões VNTR (Variable Number of Tandem Repeats) existentes no genoma de estirpes de MmmSC. O locus VNTR4 comprovou ser o mais polimórfico, detectando-se quatro perfis distintos entre as estirpes portuguesas. O perfil VNTR4 do tipo “9” (numerado em função das repetições da sequência de consenso) revelou-se amplamente distribuído geograficamente e foi predominante entre os isolados. No entanto, observou-se uma segregação geográfica de perfis, dado que na região da Beira Litoral apenas foram encontradas estirpes com o perfil VNTR4 do tipo “8”. Estes resultados sugerem que podem ter ocorrido, pelo menos, dois eventos de re-emergência de PPCB em Portugal, entre 1993 e 1998. Para a realização do segundo objectivo deste trabalho foi optimizada uma técnica baseada na hibridação in situ com sondas de DNA fluorescentes (FISH) que permitiu visualizar micoplasmas aderidos a culturas celulares. Esta técnica, que nunca tinha sido usada previamente na detecção de MmmSC, comprovou ser adequada para futuras aplicações em estudos de citoaderência e para a detecção de micoplasmas em culturas celulares.
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Caburet, Sandrine. "Structure mosai͏̈que et instabilité de l'ADN ribosomal humain : implications dans la sénescence et la cancérogenèse." Paris 7, 2002. http://www.theses.fr/2002PA077038.

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Zheng, Yun-Ling. "Rapid prenatal diagnosis of common fetal aneuploidies by fluorescence in situ hybridisation." Thesis, University of Cambridge, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.318418.

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Birchall, Philip Simon. "Multicolour fluorescence in situ hybridisation to RNA in whole-mount Caenorhabditis elegans." Thesis, University of Cambridge, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.296668.

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Mohaddes, Ardebili Seyed Mojtaba. "Optimisation of interphase fluorescence in situ hybridisation for detection of common aneuploidies." Thesis, Connect to e-thesis, 1996. http://theses.gla.ac.uk/692/.

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Thesis (Ph.D.) - University of Glasgow, 1996.
Ph.D. thesis submitted to the Faculty of Medicine, Department of Division of Developmental Medicine, University of Glasgow, 1996. Includes bibliographical references: p. 118-132. Print version also available.
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Books on the topic "FISH (Fluorescence In Situ Hybridisation)"

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Rees, Sally Anne. Fluorescence in situ hybridisation (FISH) to determine aneuploidy for chromosomes 1 and 12 in the normal male germ-line and in men exposed to radiation. Birmingham: University of Birmingham, 1997.

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Bridger, Joanna M., and Emanuela V. Volpi, eds. Fluorescence in situ Hybridization (FISH). Totowa, NJ: Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-789-1.

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Liehr, Thomas, ed. Fluorescence In Situ Hybridization (FISH). Berlin, Heidelberg: Springer Berlin Heidelberg, 2017. http://dx.doi.org/10.1007/978-3-662-52959-1.

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Liehr, Thomas. Fluorescence In Situ Hybridization (FISH) — Application Guide. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009.

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Liehr, Thomas, ed. Fluorescence In Situ Hybridization (FISH) — Application Guide. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-540-70581-9.

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Fluorescence in situ hybridization (FISH): Protocols and applications. New York, NY: Humana Press, 2010.

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Azevedo, Nuno F., and Carina Almeida, eds. Fluorescence In-Situ Hybridization (FISH) for Microbial Cells. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1115-9.

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Bysouth, James Peter Norman. Physical mapping of 45S and 5S rDNA repetitive sequences to mitotic chromosomes of Brassica species by fluorescence in situ hybridisation. Birmingham: University of Birmingham, 2003.

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O'Keeffe, Christine. Analysis of the initiation and progression of homologous chromosome pairing at meiosis in the female mouse using fluorescence in situ hybridisation. Birmingham: University of Birmingham, 1997.

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FISH handbook for biological wastewater treatment: Identification and quantification of microorganisms in activated sludge and biofilms by FISH. London: New York, 2009.

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Book chapters on the topic "FISH (Fluorescence In Situ Hybridisation)"

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Lawrie, Martin, Maria Ocana Gil, and Don Cardy. "Fluorescence in Situ Hybridisation of a Complete Set of 41 Telomere Specific Probes in a Single Simultaneous Hybridisation Experiment Using the Chromoprobe Multiprobe System-T." In FISH Technology, 301–6. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-642-56404-8_24.

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Davenport, Russell James, and Thomas Peter Curtis. "Section 7 update: Quantitative fluorescence in situ hybridisation (FISH): statistical methods for valid cell counting." In Molecular Microbial Ecology Manual, 3389–417. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-2177-0_707.

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Carter, Nigel P. "Molecular Cytogenetics: Uses of flow sorted chromosomes, fluorescence in situ hybridisation (FISH) and digital microscopy for the analysis of genomes." In Flow and Image Cytometry, 131–41. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-61115-5_10.

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Holmkvist, Lars, Jette Johanne Østergaard, and Torben Lund Skovhus. "Which Microbial Communities Are Present? Using Fluorescence In Situ Hybridisation (FISH): Microscopic Techniques for Enumeration of Troublesome Microorganisms in Oil and Fuel Samples." In Applied Microbiology and Molecular Biology in Oilfield Systems, 55–61. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-9252-6_7.

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Wolff, Daynna J. "Fluorescence In Situ Hybridization (FISH)." In The Principles of Clinical Cytogenetics, 415–39. New York, NY: Springer New York, 2012. http://dx.doi.org/10.1007/978-1-4419-1688-4_17.

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Lawce, Helen J., and Jeffrey S. Sanford. "Fluorescence in situ hybridization (FISH)." In The AGT Cytogenetics Laboratory Manual, 717–831. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2017. http://dx.doi.org/10.1002/9781119061199.ch16.

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Lee, Natuschka M., Daniela B. Meisinger, Michael Schmid, Michael Rothballer, and Frank E. Löffler. "Fluorescence In Situ Hybridization (FISH)." In Encyclopedia of Geobiology, 373–93. Dordrecht: Springer Netherlands, 2011. http://dx.doi.org/10.1007/978-1-4020-9212-1_91.

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Weise, Anja, Kristin Mrasek, Mkrtchyan Hasmik, Madeleine Gross, Sophie Hinreiner, Witthuhn Vera, and Thomas Liehr. "Parental Origin Determination FISH: Pod-FISH." In Fluorescence In Situ Hybridization (FISH) — Application Guide, 293–97. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-540-70581-9_26.

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Ye, Christine J., Lesley Lawrenson, Guo Liu, Joshua Stevens, Steve Bremer, Karen J. Ye, and Henry H. Q. Heng. "Simultaneous Fluorescence Immunostaining and FISH." In Fluorescence In Situ Hybridization (FISH) — Application Guide, 193–216. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-540-70581-9_19.

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Liehr, Thomas, Kristin Mrasek, Nadezda Kosyakova, Heike Nelle, Vladimir Trifonov, Marina Manvelyan, and Anja Weise. "FISH Banding Techniques." In Fluorescence In Situ Hybridization (FISH) — Application Guide, 243–50. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-540-70581-9_22.

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Conference papers on the topic "FISH (Fluorescence In Situ Hybridisation)"

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Galletti, M. P., F. S. Pavone, F. Vanzi, M. Capitanio, and L. Gardini. "Fluorescence in situ hybridization (FISH) with single molecule sensitivity in bacteria." In 2015 Fotonica AEIT Italian Conference on Photonics Technologies. Institution of Engineering and Technology, 2015. http://dx.doi.org/10.1049/cp.2015.0175.

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Chauhan, Hemma, Vasilica Plaiasu, Ozunu Diana, and Motei Gabriela. "P59 Clinical diversity in 22q11.2 microdeletion syndrome & difficulties in diagnoses using only fluroscence in situ hybridisation (fish)." In 8th Europaediatrics Congress jointly held with, The 13th National Congress of Romanian Pediatrics Society, 7–10 June 2017, Palace of Parliament, Romania, Paediatrics building bridges across Europe. BMJ Publishing Group Ltd and Royal College of Paediatrics and Child Health, 2017. http://dx.doi.org/10.1136/archdischild-2017-313273.147.

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Dursun, Gizem, and Ufuk Ozkaya. "Microscopic Fluorescence In Situ Hybridization (FISH) Image Synthesis with Generative Adversarial Networks." In 2021 29th Signal Processing and Communications Applications Conference (SIU). IEEE, 2021. http://dx.doi.org/10.1109/siu53274.2021.9477999.

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Kabakci, Kaan A., Abdulkerim Capar, B. Ugur Toreyin, Mertkan Akkoc, Ozan Borazan, Ilknur Turkmen, and Lutfiye Durak Ata. "A multi-level thresholding based segmentation method for microscopic fluorescence in situ hybridization (FISH) images." In 2016 24th Signal Processing and Communication Application Conference (SIU). IEEE, 2016. http://dx.doi.org/10.1109/siu.2016.7495873.

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Kurz, C. M., S. v.d. Moosdijk, H. Thielecke, and T. Velten. "Towards a cellular multi-parameter analysis platform: Fluorescence in situ hybridization (FISH) on microhole-array chips." In 2011 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 2011. http://dx.doi.org/10.1109/iembs.2011.6092074.

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Li, Jingyao, Dongdong Lin, Hongbao Cao, and Yu-Ping Wang. "Classification of multicolor fluorescence in-situ hybridization (M-FISH) image using structure based sparse representation model." In 2012 IEEE International Conference on Bioinformatics and Biomedicine (BIBM). IEEE, 2012. http://dx.doi.org/10.1109/bibm.2012.6392672.

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Kulik, M. J., D. S. Shenoda, and C. R. Forest. "A Low-Cost, Two-Axis, Precision Robot for Automated Fluorescence In-Situ Hybridization Assays." In ASME 2009 International Mechanical Engineering Congress and Exposition. ASMEDC, 2009. http://dx.doi.org/10.1115/imece2009-13272.

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Abstract:
Genetics research often relies on experiments that require repetitive, time-consuming handling of small volumes of liquid (1 mL) and biomass (10–20 μL) such as fluorescence in-situ hybridization (FISH), β-galactosidase staining, immunohisto chemistry, skeletal and tunel assays. Often manual, these experiments are time intensive and error-prone. We report on the design, fabrication, and testing of a low-cost, two-axis, precision robot for FISH assays on whole mice embryos. The robot can complete 20 successive embryo immersions in unique isothermal solutions in minutes for 6 samples. Repeatability of the orthogonal axes is 66 and 214 μm, near the measurement uncertainty limit and sufficient for operation. Accuracy is achieved by systematic error compensation. Low-cost and precision are obtained using design and manufacturing techniques and processes, resulting in a cost of 15% of comparable instruments (e.g., InsituStain, Intavis Bioanalytical Instruments). This design demonstrates a simple, automated platform to perform a typically manual experimental genetics technique.
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Johansson, Hans E., Arturo V. Orjalo, Swati Dadhich, and Myung Hee Park. "Abstract 4209: Hypusine pathway gene expression examined by single molecule RNA fluorescence in situ hybridization (smRNA-FISH)." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-4209.

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Penault-Llorca, F., B. Lannes, A. Vincent-Salomon, G. MacGrogan, M. Vilain, V. Fermeaux, I. Treilleux, et al. "Fluorescence in situ hybridization (FISH) technique by reference centers for HER2 status determination in metastatic breast cancer: quality assurance results of FISH 2002 study." In CTRC-AACR San Antonio Breast Cancer Symposium: 2008 Abstracts. American Association for Cancer Research, 2009. http://dx.doi.org/10.1158/0008-5472.sabcs-2069.

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Speel, Ernst-Jan M., Anke Haugg, Naga Deepa Pantulu, Christian Pallasch, Anna K. Kurz, Ahmad Kassem, Lukas Frenzel, et al. "Abstract 2717: Detection of Merkel cell polyomavirus in chronic lymphocytic leukemia cells by fluorescence in situ hybridization (FISH)." In Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1538-7445.am2011-2717.

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Reports on the topic "FISH (Fluorescence In Situ Hybridisation)"

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Neft, R. E., J. L. Rogers, and S. A. Belinsky. Feasibility of using fluorescence in situ hybridization (FISH) to detect early gene changes in sputum cells from uranium miners. Office of Scientific and Technical Information (OSTI), December 1995. http://dx.doi.org/10.2172/381380.

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