Journal articles: 'Fish histopathology'

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Liebel, S., M. E. M. Tomotake, and C. A. Oliveira-ribeiro. "Fish histopathology as biomarker to evaluate water quality." Ecotoxicology and Environmental Contamination 8, no. 2 (December 6, 2013): 9–15. http://dx.doi.org/10.5132/eec.2013.02.002.

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Grizzle, John M. "Introduction to Fish Gill Histopathology." North American Journal of Aquaculture 62, no. 1 (January 2000): 78–79. http://dx.doi.org/10.1577/1548-8454(2000)062<0078:br>2.0.co;2.

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Vethaak, AD. "Gross pathology and histopathology in fish: summary." Marine Ecology Progress Series 91 (1992): 171–72. http://dx.doi.org/10.3354/meps091171.

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Insivitawati, Era, Gunanti Mahasri, and Kusnoto Kusnoto. "Gambaran Darah dan Histopatologi Insang, Usus Dan Otak Ikan Koi (Cyprinus carpio Koi) yang Diinfeksi Spora Myxobolus koi secara Oral [Haematology and Histopatology of Gills, Intestine And Brain Koi Fish (Cyprinus carpio Koi) Myxobolus koi Orally Infected]." Jurnal Ilmiah Perikanan dan Kelautan 7, no. 2 (January 13, 2019): 225. http://dx.doi.org/10.20473/jipk.v7i2.11210.

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Abstract Koi fish (Cyprinus carpio koi) is one of the ornamental fish are preferred. One of the obstalces in the koi fish is a disease caused by infection myxobollus. This research to determine the effect of the infection myxobollus by oral in blood picture and histopathology. Haematology and histopathology is an indicator of the health of the fish to diagnose disease and to determine changes in the fish tissue. The research was conducted in Politeknik Kelautan dan Perikanan, dan Balai Karantina Ikan Kelas I, Juanda Surabaya from October to December 2014. This study uses the 5 treatments and 5 replications with different doses of infection (0,60,80,100 and 120 spora/fish). Data were analyzed with Annova haematology, followed by Duncan 5%. While histopathology were analyzed by semiquantitative scoring mothod used to determine the degree of histology Kluschal Wallis. The result showed that the haematology has significant result, white histopathology signify the presence of significant damage.

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Oliveira de Sales, Romário, Juliana Rosa Carrijo Mauad, Claucia Aparecida Honorato, Kesia Esther Da Silva, Jaqueline Verconti, Peceu Magyve Ragagnin, Sibele Borsuk, Mauricio Laterça Martins, and Simone Simionatto. "Histopathology and molecular identification of Henneguya pseudoplatystoma." Latin American Journal of Aquatic Research 48, no. 2 (May 6, 2020): 207–13. http://dx.doi.org/10.3856/vol48-issue2-fulltext-2345.

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The present study proposes to characterize the parasites isolated during the initial phase of production in fish farms located in Mato Grosso do Sul in the central-western region of Brazil, using histopathology analysis and molecular techniques. A total of 340 hybrid surubim fish (Pseudoplatystoma reticulatum × P. corruscans) from four farms were examined during the co-feeding phase. Histopathology analysis showed that 10.9% (n = 37) of the fish were infected with parasites. Branchitis, lifting epithelium, hypertrophy of epithelial cells, lamellar fusion, aneurisms and infection in the bone tissue of the gill filament was observed. The parasite species was determined by amplification of the 18S rRNA gene followed by sequencing. The phylogenetic analysis of nucleotide sequences indicates a close relationship (99.6%) with Henneguya pseudoplatystoma reported to be infecting the hybrid Pseudoplatystoma. This study demonstrates the occurrence of H. pseudoplatystoma in hybrid surubim (P. reticulatum × P. corruscans) during the co-feeding phase in fish farms in Mato Grosso do Sul. Also, molecular techniques provide a faster and sensitive method to identify fish parasites, and may assist in the development of new management techniques aimed at improving the sanitary conditions contributing to the reduction of mortality rates in these animals.

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Yousaf, Muhammad Naveed, and Mark D. Powell. "The Effects of Heart and Skeletal Muscle Inflammation and Cardiomyopathy Syndrome on Creatine Kinase and Lactate Dehydrogenase Levels in Atlantic Salmon (Salmo salarL.)." Scientific World Journal 2012 (2012): 1–9. http://dx.doi.org/10.1100/2012/741302.

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Heart and skeletal muscle inflammation (HSMI) and cardiomyopathy syndrome (CMS) are putative viral cardiac diseases of Atlantic salmon. This study examined the levels and correlated the serum enzymes creatine kinase (CK) and lactate dehydrogenase (LDH) to the histopathology of clinical outbreaks of HSMI and chronic CMS in farmed Atlantic salmon. A total of 75 fish from 3 different HSMI outbreaks, 30 chronic CMS fish, and 68 fish from 3 nondiseased fish groups were used as the study population (N=173). Serum CK and LDH levels correlated significantly with the total inflammation and total necrosis scores for HSMI fish (P=0.001). However, no correlation was identified for enzyme levels and histopathology scores for chronic CMS fish. The significantly increased CK and LDH levels and their positive correlations to histopathology differentiate HSMI from CMS clinically suggesting the potential use of enzymes for screening for HSMI is promising.

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Riani, Etty. "KONTAMINASI MERKURI (Hg) DALAM ORGAN TUBUH IKAN PETEK (Leiognathus equulus) DI PERAIRAN ANCOL, TELUK JAKARTA." Jurnal Teknologi Lingkungan 11, no. 2 (December 2, 2016): 313. http://dx.doi.org/10.29122/jtl.v11i2.1216.

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Mercury (Hg) which is contained in aquatic ecosystem can enter and be accumulated to organism’s body, like on petek fish (Leiognathus equulus). The research aimed to see mercury concentration in aquatic ecosystem, to see mercury concentration in organ of petek fish by AAS (Atomic Absorption Spectrophotometer) and its histopathology response in organs of petek fish. The research was done at Ancol, Jakarta Bay on October-December 2004. There are 16 samples of water and fish from 3 stations.The analysis result of water quality is compared by standard quality of sea water for sea organism life (Kepmen LH No. 51, 2004), mercu ry concentration in fish organ is compared by maximum mercury concentration in fish body by classification of Palar (2004). Ancol water quality is still on normal condition. Mercury concentration in water and petek fish organs had low concentration. Gill and lever contaminated by mercuryis only in station 1 fish, but it is still on normal concentration. Histopathology of petek fish gill is not abnormal, while the lever is necrosis. Water and petek fish is not a good indicator to detect mercury in aquatic ecosystem.Keywords: mercury, petek fish, water, lever, gill, histopathology, accumulation,indicator

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Feist, S. W., and M. Longshaw. "Histopathology of fish parasite infections - importance for populations." Journal of Fish Biology 73, no. 9 (December 2008): 2143–60. http://dx.doi.org/10.1111/j.1095-8649.2008.02060.x.

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Lutfiyah, Lailatul. "Sublethal Toxicity of Organophosphate Pesticides and its Effect on Hematology Parameter, Histopatology Hematopoietic Organ of Silver Rasbora (Rasbora argyrotaenia)." Journal of Aquaculture Science 5, no. 2 (October 26, 2020): 68. http://dx.doi.org/10.31093/joas.v5i2.94.

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Pesticides are pollutants that are found in rice fields and rivers. Pesticides that are often used by farmers in Indonesia in eradicating insects are organophosphate insecticides, where they can eradicate insects that are very toxic to fish due to strong neurotoxic substances that inhibit AchE (Acetylcholinesterase) activity. The research aims to examine the effect of organophosphate pesticides on hematology and histopathology of hematopoietic organs in silver rasbora fish. The research method used is an experimental method with a CRD. The parameters observed were hematology and histopathology hematopoietic organ (liver and kidney). The results of this study showed a hematological change in silver rasbora fish where there was a decrease in total erythrocytes (0,59±0,004) and hemoglobin (2,5±0,1) while total leukocytes increased (245,35±15,78). Also, there are differential changes in leukocytes, namely an increased in the number of monocytes (5±1) and neutrophils (24±3), but lymphocytes have decreased in number (72±1). The Histopathology of the fish liver also can found in this research, those damages that are found are erythrocyte infiltration, necrosis picnosis, and karyolysis. Histopathology of fish kidney also can found cloudy sweling, necrosis karyolysis and tubular necrosis.

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El-Mansy, Amina. "Histopathology of farmed freshwater fish infested with different helminthes." Egyptian Journal of Aquatic Biology and Fisheries 15, no. 1 (January 1, 2011): 1–13. http://dx.doi.org/10.21608/ejabf.2011.2072.

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Rodriguez-Nuñez, Juan, Francisco J. Avelar, Francisco Marquez, Bruno Rivas-Santiago, Cesar Quiñones, and Alma L. Guerrero-Barrera. "Mycobacterium tuberculosis complex detected by modified fluorescent in situ hybridization in lymph nodes of clinical samples." Journal of Infection in Developing Countries 6, no. 01 (November 30, 2011): 58–66. http://dx.doi.org/10.3855/jidc.1752.

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Introduction: Lymph node tuberculosis (TB) is the leading cause of extrapulmonary tuberculosis and is the most frequently identified type in Aguascalientes, Mexico. Conventional diagnosis has serious limitations for rapid detection of extrapulmonary tuberculosis in clinical samples. Here PCR and modified FISH have been tested as complementary diagnosis methods for extrapulmonary tuberculosis. Methodology: The specific insertion sequence IS6110 for Mycobacterium tuberculosis complex was used to perform PCR and build DNA and PNA FISH probes (20bp). PCR and modified DNA and PNA FISH assays were performed to evaluate 41 lymph node paraffin-embedded tissue samples, in comparison with the histopathology diagnosis, which was considered the gold standard (22 positive and 19 negative). Results: In comparison with histopathology diagnosis PCR showed 62.5 % sensitivity and 77.8 % specificity (χ2 = 4.583 p < 0.05). Modified DNA FISH showed 71.4% sensitivity and 84.6% specificity (χ2 = 11.21 p < 0.05). PNA FISH showed 66.7% sensitivity and 60.0% specificity (χ2 = 2.93 p > 0.05). Ziehl Neelsen stain was positive in only four cases of 22 lymph node samples positive to histopathology. In contrast, PCR and modified DNA FISH were positive in 20 cases of the same group. The negative cases were coincident in all tests.Conclusions: PCR and DNA FISH showed a significant increase in the number of cases detected and also showed higher sensitivity and specificity compared with data reported by traditional methodology. In developing countries, these techniques could help to complement the early diagnosis and timely treatment of extrapulmonary tuberculosis.

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Magor, Brad G. "Gill histopathology of juvenile Oncorhynchus kisutch exposed to suspended wood debris." Canadian Journal of Zoology 66, no. 10 (October 1, 1988): 2164–69. http://dx.doi.org/10.1139/z88-323.

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Gill histopathology was examined in three groups of parr of coho salmn (Oncorhynchus kisutch (Walbaum)) maintained for 30 days in suspended wood debris (< 1.00 mm diameter; concentrations of 44–132 mg/L dry weight) collected from an estuarine log sorting site. Gills of fishes were examined by light microscopy following exposure, and histopathologic responses were indexed by percentage of gill filaments affected. Lamellar edema and telangiectasis each had statistically significant (P < 0.05) associations with exposure to the suspended debris, and the severity of these conditions was positively correlated with increased concentrations of debris. The severity of hyperplasia of the primary filament epithelium did not correlate with the concentration of debris. The total number of gill lesions in each fish (sum of hyperplasia, telangiectasis, and edema) was positively correlated with the concentration of debris to which the fish were exposed, and negatively correlated with the percent weight increment (((final weight – initial weight)/initial weight) × 100) of fishes over the experimental period.

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Koponen, Kari, Mark S. Myers, Ossi Ritola, Sirpa E. Huuskonen, and Pirjo Lindström-Seppä. "Histopathology of Feral Fish from a PCB-Contaminated Freshwater Lake." AMBIO: A Journal of the Human Environment 30, no. 3 (May 2001): 122–26. http://dx.doi.org/10.1579/0044-7447-30.3.122.

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Al-Zaidan, A. S., H. A. Al-Sarawi, M. S. Massoud, M. Al-Enezi, A. J. Smith, J. P. Bignell, M. J. Green, et al. "Histopathology and contaminant concentrations in fish from Kuwait’s marine environment." Marine Pollution Bulletin 100, no. 2 (November 2015): 637–45. http://dx.doi.org/10.1016/j.marpolbul.2015.07.030.

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Jabeen, F., and A. S. Chaudhry. "Heavy metal uptake, bioaccumulation and histopathological responses in gills of freshwater fish from the Indus River, Pakistan." Proceedings of the British Society of Animal Science 2009 (April 2009): 127. http://dx.doi.org/10.1017/s1752756200029665.

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The high nutritional value of fish makes it an ideal component of a healthy and balanced diet. Elevated levels of heavy metals in fish represent both an ecological and human health concern. Fish species are often the top consumers in aquatic ecosystems and thus metal concentrations in fish can act as an indicator of the state of the aquatic environment. Fish are known to bio-accumulate metals and are considered as one of the most indicative factors, in freshwater systems, for the estimation of trace metals pollution potential (Rashed, 2001). Histopathology can be an indicator of the effects of various anthropogenic pollutants on organisms and so could be used as a reflection of the overall health of the entire population in an ecosystem. This study aimed to assess the bioaccumulation of heavy metals in gills and muscles and their effects on histopathology of gills of Oreochromis mossambicus from the Indus River, Pakistan which receives potential pollutants from the domestic, municipal and agricultural runoffs.

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& et al., Mustafa. "HISTOPATHOLOGYANDLEVELOF BIOACCUMULATION OFSOME HEAVY METALS IN FISH, CARASOBARBUSLUTEUS AND CYPRINUSCARPIOTISSUES CAUGHTFROM TIGRIS RIVER, BAGHDAD." IRAQI JOURNAL OF AGRICULTURAL SCIENCES 51, no. 2 (April 26, 2020): 698–704. http://dx.doi.org/10.36103/ijas.v51i2.997.

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This study was undertaken to detect the histopathology and level of bioaccumulation of lead and cadmium in water and in some freshwater fish (Carasobarbus luteus and Cyprinus carpio) tissues caught from Tigers River. A total of 100 water samples and 100 fish specimens (kidney, gills, liver and muscle) were collected from three sites of Tigris River. These samples were used to detect Pb and Cd via Atomic Absorption Spectrophotometry.Results exhibited high concentrations of Pb and Cd in water samples (>0.03 for both metals) and insome organs of the selected fish. It was observed that the levels of Pb and Cd accumulated in most organs (kidneys being most influenced) followed by gills, livers and muscles at three sites. The levels of these heavy metals were much above the maximum acceptable limit recommended by FAO and WHO. Histopathology was also conducted where heavy damages were noticed in both livers and gills in both fish species. The current study present data about increasing pollution in the Tigris River and it approves that it is having strong impact on fish health and on human beings.

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Kirk, R. S., and J. W. Lewis. "Histopathology of Sanguinicola inermis infection in carp, Cyprinus carpio." Journal of Helminthology 72, no. 1 (March 1998): 33–38. http://dx.doi.org/10.1017/s0022149x00000948.

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AbstractThe histopathological response of carp to Sanguinicola inermis was investigated by serial sectioning laboratory infected fish up to 90 days post infection (d p.i.). Juvenile flukes and adults caused mechanical damage to tissues during invasion and migration up to 28 d p.i. Adults partially occluded blood vessels and may have reduced blood circulation. In the initial phase of egg production (28–42 d p.i.), eggs and emigrating miracidia in gill tissue caused breakdown of vascular integrity, necrosis, hyperplasia, haemorrhage and eosinophilic infiltration of epithelial tissue. After 42 d p.i. the host granulomatous inflammatory response encapsulated eggs lodged in the gills, visceral sites and connective tissue displacing normal tissue. Encapsulation and subsequent degradation of eggs and miracidia within granulomata was highly developed by 90 d p.i. Laboratory infections of S. inermis can induce respiratory distress and therefore impair respiration of fish. The parasite also caused pathological changes in osmoregulatory, excretory and haemopoietic tissue and may impair function in these organ systems.

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Bazh, E. K. A., and A. H. Hamouda. "Scanning morphology, prevalence and histopathology of some acanthocephalans infecting some River Nile fish." BULGARIAN JOURNAL OF VETERINARY MEDICINE 24, no. 2 (2021): 239–50. http://dx.doi.org/10.15547/bjvm.2019-0087.

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Acanthocephalan morphology and their adverse pathological impact on fish are of great concern. Two species of acanthocephalans were recorded from 800 samples of live freshwater fish collected randomly during 2017–2018 from Lake Nasser, Aswan, Egypt. The recovered species were identified morphologically as Acanthogyrus (Acanthosentis) tilapiae from three Tilapia spp. (Sarotherodon galilaeus, Oreochromis niloticus and Tilapia zillii) and Rhadinorhynchus niloticus from Lates niloticus. The intensity of parasitic infection and the seasonal prevalence were higher in L. niloticus than in Tilapia spp. The clinical signs and post mortem lesions of infected fish were reported. Morphological description of the detected parasites using light microscope was then confirmed by electron microscopy to amplify ambiguous details. The histopathological findings of the intestine of naturally infected fish with acanthocephalan parasites were investigated and described. The main damage caused by them is destruction of the mucosal epithelium of the villi, necrosis and degeneration of intestinal epithelial cells.

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Silva, Weliton Vilhalba da, Henrique Momo Ziemniczak, Flávia Barbieri Bacha, Rudã Brandão Santos Fernandes, Rodrigo Yudi Fujimoto, Rebeca Maria Sousa, Klaus Casaro Saturnino, and Claucia Aparecida Honorato. "Respiratory profile and gill histopathology of Carassius auratus exposed to different salinity concentrations." Semina: Ciências Agrárias 42, no. 5 (July 2, 2021): 2993–3006. http://dx.doi.org/10.5433/1679-0359.2021v42n5p2993.

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The aim of this study was to evaluate the chronic salinity tolerance of Carassius auratus and the effects on blood parameters, gill morphology, and survival. In the first test, nine different concentrations (0.0, 0.5, 1.0, 2.5, 5.0, 10, 15, 20, and 25 g L-1) of NaCl were used with nine repetitions for 96 h. The survival of fish subjected to 15 g L-1 NaCl was 4 h, and 5 min at a concentration of 25 g L-1. The mortality of fish with 15 g L-1 NaCl was 100%. Morphological analyses of the gills showed hyperplasia of the coated cells in the interlamellar space and hypersecretion of mucus in fish exposed to 10 g L-1 of NaCl. At concentrations of 20 and 25 g L-1, necrosis of the support collagen caused the cells to detach from the lamellar structure mucosa. In the chronic test, two concentrations were used, with four replications containing nine fish in each aquarium for a period of 21 days. Blood samples and gills from the fish were collected, and it was observed that the fish showed a decrease in the concentration of bicarbonate (NaHCO3) in the blood, indicating hypernatremia. Acute exposure of C. auratus to sodium chloride (NaCl) should be at a maximum of 10 g L-1 of NaCl, after which level there would be a loss in animal performance and/or mortality. Chronic exposure to 5 g L-1 of NaCl promotes acidemia, ionic imbalance, and pathological changes in the gills; therefore, it is not recommended.

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Fonseca, A. R., L. F. Sanches Fernandes, A. Fontainhas-Fernandes, S. M. Monteiro, and F. A. L. Pacheco. "From catchment to fish: Impact of anthropogenic pressures on gill histopathology." Science of The Total Environment 550 (April 2016): 972–86. http://dx.doi.org/10.1016/j.scitotenv.2016.01.199.

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Carvalho, Cristina, Vasco Branco, Paula Ramos, João Canário, Jun Lu, Arne Holmgren, and Cristina Carvalho. "Histopathology vs. inhibition of the thioredoxin system of mercury-exposed fish." Toxicology Letters 211 (June 2012): S120. http://dx.doi.org/10.1016/j.toxlet.2012.03.442.

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Bernet, D., H. Schmidt, W. Meier, P. Burkhardt-Holm, and T. Wahli. "Histopathology in fish: proposal for a protocol to assess aquatic pollution." Journal of Fish Diseases 22, no. 1 (January 1999): 25–34. http://dx.doi.org/10.1046/j.1365-2761.1999.00134.x.

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23

Lindesjöö, E., and J. Thulin. "Histopathology of skin and gills of fish in pulp mill Effluents." Diseases of Aquatic Organisms 18 (1994): 81–93. http://dx.doi.org/10.3354/dao018081.

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Santos, Monyele Acchile, Gabriela Tomas Jerônimo, Lucas Cardoso, Karen Roberta Tancredo, Paula Brando Medeiros, José Victor Ferrarezi, Eduardo Luiz Tavares Gonçalves, Guilherme da Costa Assis, and Maurício Laterça Martins. "Parasitic fauna and histopathology of farmed freshwater ornamental fish in Brazil." Aquaculture 470 (March 2017): 103–9. http://dx.doi.org/10.1016/j.aquaculture.2016.12.032.

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Wakawa, A. I., and S. B. Audu. "Histopathological alterations in gills, kidney and liver of Nile Tilapia (Oreochromis niloticus) fingerlings exposed to aqueous leaf extract of Desert Date (Balanites aegyptiaca)." Zoologist (The) 18, no. 1 (April 8, 2021): 67–73. http://dx.doi.org/10.4314/tzool.v18i1.12.

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One of the many biomarkers for determining the effects of pollutants on fish is changes in organ histopathology. Leaves of Balanites aegyptiaca have been reported to have phytochemicals with fish anaesthetic property. This study sought to determine the effect of graded acute concentrations (200.00, 250.00, 300.00.350.00 and 400.00 mg/L) of B. aegyptiaca on histopathology of gills, kidney and liver of mixed sex of Oreochromis niloticus fingerlings. A total of 120 O. niloticus fingerlings (mean weight 23±0.03 g and mean total length 12.50±0.39 cm) were exposed to the plant extract. Paraffin wax method and haematoxylin-eosin staining techniques of tissue processing were adopted for the examination of the gills, kidney and liver. Dose-dependent histopathological changes were observed in the three organs (gills, kidney and liver) i.e. histopathological alterations increase with increase in concentration of the plant extract. Gills showed lamellae fusion, haemorrhage, desquamation, atrophy and secondary lamellae erosion while kidney and liver indicated atrophy, necrosis, haemorrhage, hyperplasia and hypertrophy. Structural alterations were evident in the gills, kidney and liver of O. niloticus fingerlings exposed to the concentrations of aqueous crude leaf extract of B. aegyptiaca therefore it should be used with caution during fish anaesthesia. Keywords: Histopathology; Gill; Kidney; Liver; Balanites aegyptiaca; Oreochromis niloticus.

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Jerônimo, GT, SB Pádua, D. Bampi, ELT Gonçalves, P. Garcia, MM Ishikawa, and ML Martins. "Haematological and histopathological analysis in South American fish Piaractus mesopotamicus parasitized by monogenean (Dactylogyridae)." Brazilian Journal of Biology 74, no. 4 (November 2014): 1000–1006. http://dx.doi.org/10.1590/1519-6984.09513.

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Monogeneans are the parasites mostly found on the body surface and gills of fish and can cause large losses in farmed fish. Some studies demonstrate elevated parasitic levels causing hematological alterations. But few of them relate the effects of parasitism on the hematology and histopathology of native freshwater farmed fish. This study evaluated the host-parasite relationship in pacu (Piaractus mesopotamicus) parasitized by the monogenean Anacanthorus penilabiatus. Hematological and parasitological assessments were obtained in 60 fish captured in a fish farm located in Dourados, State of Mato Grosso do Sul, Central Brazil. Fish were analyzed in different categories of parasite number: class I (n=13; 0-200 parasites), class II (n=17; 201-1200 parasites); class III (n=7; 1201-2200 parasites); and class IV (n=23; more than 2200 parasites per host). The highest levels of parasitism caused significant decrease (p<0.05) in the hematocrit, red blood cells (RBC), mean hemoglobin concentration (MCHC) and basophils number. Thrombocytes, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), monocytes, eosinophils, neutrophils and LG-PAS did not present significant difference among the parasitic levels. In contrast, increased number of total leukocytes and lymphocytes were found in highly-parasitized fish. A positive linear correlation (p<0.01) was found between the amount of parasites and fish weight. Histopathology revealed severe hyperplasia, sub-epithelial edema, fusion of the secondary lamellae, focal and multifocal necrosis in highly parasitized fish.

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Deshpande, A. S., S. B. Zade, and S. R. Sitre. "Histopathological changes in the gill architecture of Labeo rohita from the pond adjacent to thermal power station, Koradi, Nagpur, India." Journal of Applied and Natural Science 3, no. 2 (December 1, 2011): 284–86. http://dx.doi.org/10.31018/jans.v3i2.198.

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The hazardous effect of water pollution on the histopathology of selected organs of the freshwater fish Labeo rohita was studied with respect to thermal power plant effluent. The purpose of this research paper is to study whether the thermal plume can affect fish assemblages in the waters around the outlet area of the thermal power plant located at Koradi near Nagpur city. Both experimental and control fishes were monitored for a year from March 2010 to Feb. 2011. Histopathology serve as a tool to assess the extent of damage caused to the organism.Gill tissue showed fusion of primary lamellae, congestion of blood vessels and hyperplasia of bronchial plates. The histopathological changes in the gill tissue include epithelial hyperplasia, curling of secondary lamellae and changes in chloride cells. Besides these changes pyknotic nuclei, vacuolization, degradation of epithelial and pillar cells, were noticed, which point out towards deleterious effect of thermal power plant effluent on the freshwater fish L. rohita.

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Slaninova, A., J. Machova, and Z. Svobodova. "Fish kill caused by aluminium and iron contamination in a natural pond used for fish rearing: a case report ." Veterinární Medicína 59, No. 11 (December 9, 2014): 573–81. http://dx.doi.org/10.17221/7821-vetmed.

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Contamination of Pansky Pond, in March 2013, with 119 mg/l aluminium, and 87 mg/l iron by acidic (pH 3.17) inflow from a nearby quarry caused fish die-off, while exhibiting symptoms of suffocation. Transformation of soluble forms of aluminium and iron into insoluble forms occurred on fish gill where the content of aluminium and iron was 100-fold and 12-fold, respectively, that found in control fish in an unaffected pond. In addition to insoluble aluminium and iron, gills showed presence of iron bacteria. Histopathology was characterised by expression of reactive processes and regressive alterations resulting in gill tissue necrosis. Impairment of the excretory function of gills was reflected in significantly (P < 0.01) higher concentrations of ammonia in the blood plasma of exposed fish compared to the control. Damage to parenchymatous tissues (kidney, liver, spleen) of the exposed fish was manifested as dystrophic alterations, higher aluminium and iron content, and enhanced activity of transaminases in blood plasma compared to the control.

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Wolf, Jeffrey C., Wes A. Baumgartner, Vicki S. Blazer, Alvin C. Camus, Jeffery A. Engelhardt, John W. Fournie, Salvatore Frasca, et al. "Nonlesions, Misdiagnoses, Missed Diagnoses, and Other Interpretive Challenges in Fish Histopathology Studies." Toxicologic Pathology 43, no. 3 (August 11, 2014): 297–325. http://dx.doi.org/10.1177/0192623314540229.

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KE, Xiao-Li, Jian-Guo WANG, Jing YUAN, and Xiao-Ning GONG. "STUDIES ON THE PATHOGENICITY AND HISTOPATHOLOGY OF FUSARIUM IN FISH." Acta Hydrobiologica Sinica 36, no. 5 (September 17, 2010): 943–48. http://dx.doi.org/10.3724/sp.j.1035.2010.00943.

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Thiyagarajah, Arunthavarani, William R. Hartley, Sharee E. Major, and Michael W. Broxson. "Gill histopathology of two species of buffalo fish from a contaminated Swamp." Marine Environmental Research 42, no. 1-4 (June 1996): 261–66. http://dx.doi.org/10.1016/0141-1136(95)00054-2.

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Hoseini, Seyyed Morteza, Behrooz Gharavi, and Yousef Iri. "Assessment of vital organ histopathology and plasma oxidative conditions of rainbow trout Oncorhynchus mykiss reared in earthen saltwater pond." RUDN Journal of Agronomy and Animal Industries 14, no. 3 (December 15, 2019): 255–65. http://dx.doi.org/10.22363/2312-797x-2019-14-3-255-265.

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The aim of this study was to compare gill, kidney, liver and gut histopathology, and plasma antioxidant markers of rainbow trout Oncorhynchus mykiss reared in saltwater earthen ponds in Gomishan, Iran. To this, 10000 fish were distributed in a three-ha earthen pond and 150 fish in three fiberglass tanks (2000L). Blood samples were taken after 3 months rearing with same commercial feed. The source of fish and feed was similar between the saltwater pond and fiberglass tanks. After the 3-month rearing, gill, kidney, liver and gut samples were taken from the pond fish; whereas, blood samples were taken from both the pond and tank fish. There was no significant difference in water temperature, dissolved oxygen and pH between the pond and tanks; however, water salinity and ammonia was higher in the pond compared to the tanks. Plasma superoxide dismutase and glutathione peroxidase activity of the fish in earthen ponds were significantly higher than those fish reared in fiberglass tanks; however, there was no significant in thiobarbituric acid reactive substances between the pond and tanks. The fish had various histopathological symptoms including primary and lamella hyperplasia, lamellar fusion and epithelial lifting. In the kidney section, the fish showed glomerulus shrinkage and/or disappearance, melanomacrophage aggregates and hematopoietic tissue necrosis. These fish showed necrosis and melanomacrophage aggregates in liver and goblet cell hypertrophy in gut. The results suggest that the fish in the earthen pond faced stressful conditions, which might be due to water salinity and ammonia; however, other possible factors, such as pollutants and different feeding regimen must be considered.

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Sa’idu Aliyu, Bashir, Sohnap James Sambo, and Ochuko Orakpoghenor. "Effect of Partial Feed Deprivation on Serum Liver Enzymes’ Activities and Hepatic Histoarchitecture in Clarias gariepinus (African Catfish)." Academic Journal of Life Sciences, no. 71 (March 23, 2021): 22–27. http://dx.doi.org/10.32861/ajls.71.22.27.

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The aim of this study was to evaluate serum liver enzymes’ activities and hepatic histoarchitecture in partially feed-deprived Clarias gariepinus (African Catfish). A total of forty-eight (48) active, live and apparently normal catfish randomly placed into two groups were used for this study. Fish in control group (labeled B) were fed with 4% of their body weight twice daily while fish in the feed-deprived group (labeled A) were fed with 25% of that quantity fed to the control group. Blood and liver samples were obtained at intervals of 7 days for 28 days for alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP), and for histopathology respectively. Results revealed significantly higher (P < 0.05) values for ALT, AST and ALP in the feed-deprived group (A) compared to the control group (B). On histopathology, liver sections of feed-deprived fish showed lipid vacuolation and this became accentuated over the period and most prominent on day 28. The study has demonstrated that starvation in catfish produced liver damage reflected by increase in serum activities of these liver enzymes.

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Qureshi, T., M. R. White, and C. Santrich. "Evaluation of Nested Polymerase Chain Reaction, Pepsin/Trypsin Digest, and Histology for the Detection of Myxobolus Cerebralis in Salmonid Fishes of Indiana and Michigan." Journal of Veterinary Diagnostic Investigation 14, no. 3 (May 2002): 251–54. http://dx.doi.org/10.1177/104063870201400312.

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The objectives of this study were to survey fish from state hatcheries in Indiana and Michigan and to compare the nested polymerase chain reaction (PCR) test with pepsin/trypsin digest (PTD) and histopathology for the diagnosis of whirling disease (WD). One group of 40 and 9 groups of 60 fish heads, for a total of 580 samples, were submitted from hatcheries in Indiana and Michigan. These samples were examined for myxozoan spores using histopathology, PTD, and PCR tests. The heads were hemisectioned, and one half was fixed in 10% neutral-buffered formalin for histopathologic examination. The other half was processed for PTD. Some of the sediment was examined for the presence of myxozoan spores, and the rest was prepared for the nested PCR. Histologic examinations did not reveal Myxobolus cerebralis in any of the 580 samples. One hundred serial step sections, taken at 5-μm intervals, were evaluated for samples with positive spore identification by PTD. Histologic examination of these sections failed to reveal any myxozoan parasites. Myxozoan spores were observed in 16.9% (98/580) of samples in sediment after PTD. Spores morphologically similar to those of M. cerebralis were observed in 1.0% of PTD samples ( n = 6). The nested PCR indicated that M. cerebralis spores were present in 0.5% of samples ( n = 3). All 3 nested PCR-positive samples came from the same hatchery; however, spores of M. cerebralis were seen in 1 sample, spores of other myxozoan species were seen in the second sample, and spores were not seen in the third sample. When comparing the PTD to the nested PCR test, the PTD diagnosed 1 true positive, 5 false positives, 2 false negatives, and 572 true negatives, for a sensitivity of 33% and a specificity of 99.1%. Screening for M. cerebralis infection in this study indicated a low prevalence of the disease. Histopathology was a very insensitive indicator of WD. The PCR test was highly specific and was used to differentiate spores of M. cerebralis from similar spores of other species.

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Moniruzzaman, M., MM Khan, MK Rahman, and MS Islam. "Effects of profenofos induced histopathology and recovery patterns in silver barb (Barbonymus gonionotus)." Progressive Agriculture 28, no. 3 (November 24, 2017): 240–48. http://dx.doi.org/10.3329/pa.v28i3.34661.

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Histopathology is promising field for research in aquatic toxicology as it provides the real picture of the toxic effects of xenobiotics in vital functions of a living organism. The present study aims to evaluate the toxic effect of pesticide namely profenofos on silver barb. Liver and kidney of silver barb were examined histologically after exposure to sublethal concentrations (0.01 ppm, 10% of LC50 and 0.05 ppm, 50% of LC50) of profenofos for 0, 7, 15 and 30 days. Histological recovery was also studied by maintaining the pesticide‐exposed fish in a freshwater system for an additional 7, 15 and 30 day. Kidney and liver of exposed individuals exhibited some remarkable changes in their histology in comparison to control and recovery group. Hepatic lesions in the liver tissues of fish were characterized by cloudy swelling of hepatocytes, lipoid vacuoles, pycnotic nuclei and focal necrosis. Epithelial hypertrophy, narrowing of the tubular lumen, atrophy of the glomerulus, broader Bowman's capsule, necrosis in the epithelial cells and pycnosis in the hematopoietic tissue were observed in kidney tissues of experimental fish. These lesions grew with increasing concentration. Although some of the changes were reversible, the rest were less pronounced after a recovery period.Progressive Agriculture 28 (3): 240-248, 2017

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Santos, Rudã F. B., Henrique M. Dias, and Rodrigo Y. Fujimoto. "Acute toxicity and histopathology in ornamental fish amazon bluespotted corydora (Corydoras melanistius) exposed to formalin." Anais da Academia Brasileira de Ciências 84, no. 4 (December 2012): 1001–7. http://dx.doi.org/10.1590/s0001-37652012000400014.

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The objective of this work was to evaluate the acute toxicity of formalin and histopathological effects on the Amazon ornamental fish, bluespotted coridora (Corydoras melanistius). A randomized design was used, with ten concentrations of formalin (40%) (0, 3, 6, 12, 25, 50, 100, 150, 200 and 250mg.L-1) with four replicates and five fish per container (3L) in static system for 96 hours. The moribund fish were killed and fixed in 10% formalin to proceed the histopathological analysis of gill, liver and kidney. At the end of this experiment the following mortality rates (%) were obtained in increasing order of exposure: 0, 0, 0, 0, 0, 65, 85, 100, 100 and 100%. The lethal concentration 50% (LC50-96h (I)) estimated was 50.76 mg.L-1 with regression of y = 0.51x, and r² = 0.80. Further, in higher concentrations morphological changes as gill hyperplasia, with filling of interlamellar spaces, disorganization of liver arrangement, and necrosis in kidney were observed. In this study, the formalin can be considered slightly toxic to bluespotted corydora, and cause morphological changes when exposed to high concentrations. The use of formalin to treat of ornamental fish in the inner river of capture with wrong concentration can provoke negative environmental and biological effects.

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Coleman, R. A., and A. A. Hoffmann. "Digenean trematode cysts within the heads of threatened Galaxiella species (Teleostei : Galaxiidae) from south-eastern Australia." Australian Journal of Zoology 64, no. 4 (2016): 285. http://dx.doi.org/10.1071/zo16004.

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The dwarf galaxias (Galaxiella pusilla) and little galaxias (Galaxiella toourtkoourt) are both threatened freshwater fish from south-eastern Australia. Occasionally populations have been found with enlarged heads associated with the accumulation of ‘white balls’, but the cause of these deformities has not previously been investigated. In this study, histopathology and molecular techniques were employed to identify cysts extracted from the heads of Galaxiella species across six populations. Histopathology and DNA sequences from both mitochondrial (cytochrome c oxidase subunit I) and nuclear (ribosomal internal transcribed spacer 1) regions identified the cysts as metacercariae of Apatemon gracilis (Rudolphi, 1819), a cosmopolitan digenean trematode species. Heavy infestations of trematode metacercariae within Galaxiella populations are of concern due to the potential to cause increased mortality associated with altered behaviour of the fish host that increases the likelihood of predation. Direct mortality from infestations is also possible, but not quantified in this study.

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Ciambrone, Chelsea, Laura R. Chen, Debra A. Tokarz, and Gregory A. Lewbart. "Chromatophoroma in a Siamese fighting fish (Betta splendens)." Veterinary Record Case Reports 7, no. 2 (June 2019): e000840. http://dx.doi.org/10.1136/vetreccr-2019-000840.

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An adult male blue Siamese fighting fish (Betta splendens) presented to the North Carolina State University College of Veterinary Medicine with a cranial mass located dorsal to his right eye. A fine-needle aspiration was performed followed by supportive care. Due to the patient’s small size aggressive treatment was not elected. The mass continued to expand over a course of five months until the patient was found deceased, and a postmortem examination was performed. Histopathology determined the mass to be a chromatophoroma of the iridophoroma or leucophoroma subtypes. This is the first documentation of a chromatophoroma in B splendens.

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White, Melvin Randall, Ching Ching Wu, and Sharon R. Albregts. "Comparison of Diagnostic Tests for Bacterial Kidney Disease in Juvenile Steelhead Trout (Oncorhynchus Mykiss)." Journal of Veterinary Diagnostic Investigation 7, no. 4 (October 1995): 494–99. http://dx.doi.org/10.1177/104063879500700412.

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In order to accurately diagnose bacterial kidney disease caused by Renibacterium salmoninarum in steelhead trout, kidney tissue from experimentally infected fish was evaluated using a commercially available enzyme-linked immunosorbent assay (ELISA) test kit, fluorescent antibody (FA) testing, bacteriologic culture, and histopathology. Seventy-five steelhead trout were randomly assigned to 1 of 4 groups and intraperitoneally inoculated with 0.15 ml saline ( n = 20), 1 × 1010 organisms/ml ( n = 18), 1 × 108 organisms/ml ( n = 18), or 1 × 106 organisms/ml ( n = 19) of R. salmoninarum. ELISA, FA, and bacteriologic culture were positive for R. salmoninarum from the kidney tissue of the 2 groups infected with the highest doses. Although the ELISA and FA tests were accurate when compared to the bacteriologic culture from the 2 groups infected with the higher doses of the organism, they were less sensitive at the lowest level of inoculum. Histopathology was not specific for this disease; however, all infected fish had a marked proliferative histiocytic interstitial nephritis, characterized by marked expansion of the renal hematopoietic tissue by histiocytes without tissue necrosis. Other microscopic findings included splenitis and myositis (at the injection site) of some fish.

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Swaminathan, T. R., N. Sood, P. K. Pradhan, M. K. Yadav, R. Kumar, D. K. Verma, R. Pal, A. Dharmaratnam, and N. K. Sood. "Systemic mycobacteriosis along with concurrent fibroma in Goldfish, Carassius auratus from India." Journal of Environmental Biology 42, no. 4 (July 1, 2021): 1019–25. http://dx.doi.org/10.22438/jeb/42/4/mrn-1682.

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Aim: Goldfish (Carassius auratus L.), a freshwater fish belonging to the family Cyprinidae, is an important ornamental fish species in the world. Herewith, we report a case of systemic mycobacteriosis along with concurrent fibroma in goldfish. Methodology: The protruding mass and internal organs exhibiting lesions were collected for histopathology and molecular diagnosis. The sections of cutaneous mass were stained with haematoxylin and eosin (H&E) and Masson trichrome, whereas sections from spleen as well as kidney were stained with H&E and Ziehl-Neelsen. For identifying the etiological agent, 16S rRNA gene fragment was amplified and sequenced, using genus-specific primers for Mycobacterium sp. Results: In histopathology, multiple granulomas could be observed in kidney and spleen sections. Importantly, typical acid-fast bacilli were demonstrated in granulomas in spleen. The sequence of 16S rRNA gene fragment amplified from spleen and kidney showed maximum similarity to Mycobacterium stomatepiae. Besides, the histopathological characteristics of the protruding mass were consistent with cutaneous fibroma. Interpretation: These findings assume significance as several Mycobacterium species, including the current one from ornamental fish are reported to be of zoonotic significance.

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Putri Desi Wulan Sari, Setiawan Koesdarto, Gunanti Mahasri,. "Perubahan Patologi Kulit Ikan Gurami (Osphronemu gouramy) Akibat Infestasi Lernaea cyprinacea Pada Derajat Infestasi Yang Berbeda [The Pathology Alteration Of Gouramy (Osphronemus gouramy) Integument Infested By Lernaea cyprinacea At Different Degress Of Infestation]." Jurnal Ilmiah Perikanan dan Kelautan 2, no. 2 (January 24, 2019): 123. http://dx.doi.org/10.20473/jipk.v2i2.11640.

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Abstract The development of fish consumption in Indonesia was promising increasingly and growing very rapidly. One of the priority commodities in the development of aquaculture subsector was the gourami (Osphronemus gouramy). Many factors must be faced in the cultivation of gouramy, including the problem of fish diseases caused by Lernaea cyprinacea. The aim of this research is to find out the description of anatomic pathology and histopathology of gouramy which infested by L. cyprinacea at different degrees of infestation. This research uses descriptive method. The research showed that the gouramy integument which infested by L. cyprinacea at low, moderate and heavy infestation cause moderate until heavy defect with 2.48; 2.42 and 2.32 in reaverage scoring. Hence, accordance with pathological anatomy, L. cyprinacea infestation caused necrotic nodules, lesion which covered by exudate and haemorrhage. While the histopathology of the integument changes found were inflammatory cell infiltration, congestion and haemorrhage in the low, moderate and heavy infestation.

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WAHIDI, BUDI RIANTO, UUN YANUHAR, MOHAMAD MOHAMAD FADJAR, and SRI ANDAYANI. "Pathognomonic features and ultrastructural of Koi Herpesvirus infected Oreochromis niloticus." Biodiversitas Journal of Biological Diversity 20, no. 2 (February 17, 2019): 497–503. http://dx.doi.org/10.13057/biodiv/d200228.

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Abstract. Wahidi BR, Yanuhar U, Fadjar M, Andayani A. 2019. Pathognomonic features and ultrastructural of Koi Herpesvirus infected Oreochromis niloticus. Biodiversitas 20: 497-503. Koi Herpesvirus (KHV) has caused considerable losses in the cultivation of the Cyprinidae family worldwide. In addition to infecting Cyprinidae, KHV can also infect other freshwater fish. This study reports histopathology and ultrastructural analysis of KHV that infect Nile tilapia. Although there are no specific clinical symptoms, pathognomonic indicating Nile tilapia fish has been infected with KHV have been found, i.e. through the formation of intranuclear inclusion body and cell swelling which essentially experience chromatin margination. In addition, histopathology results indicate changes or damage to other tilapia tissue, i.e. necrosis, hypertrophy, edema, hemorrhage, inflammation, congestion, degeneration, and apoptosis. These results indicate that KHV has been distributed and replicated in tilapia organs. Based on the Transmission Electron Microscopy (TEM) analysis, KHV virions have been detected in the cytoplasm or outside the cells on a size of 150-170 nm.

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Zade, S. B., D. M. Agase, A. M. Nagwnshi, H. N. Nenwani, S. N. Qureshi, and H. Jagyasi. "Studies on the toxicity of 2-Methyltetrahydrofuran on the histopathology of gills of African catfish Clarias gariepinus." Journal of Applied and Natural Science 10, no. 2 (June 1, 2018): 765–69. http://dx.doi.org/10.31018/jans.v10i2.1775.

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In the present study, investigation were carried out on gills of African cat fish Clarias gariepinus exposed to sub lethal concentrations (80mg/ml,400mg/ml and 800mg/ml) of 2-Methyltetrahydrofuran for 10 days. Lesions were observed in gills tissue of treated fish for long term exposure to Methyltetrahydrofuran (2MTHF). The occurrence and degree of alteration were positively related with the concentration of 2MTHF. Histological examination of the gills of fish treated with 80 mg/ml of 2MTHF for 10 days showed architectural loss, necrosis and desquamation of epithelial layer. Histological examination of the gills of fish treated with 400 mg/ml of 2MTHF for 10 days showed architectural loss, necrosis and mild vaccuolation. The gill filament exhibited telangiectesis, disorganisation of secondary gill lamellae and complete vaccuolation of gills treated with 800 mg/ml of 2MTHF for 10 days. The study indicated that 2MTHF had marked effects on the cyto-architecture of the gills of C. gariepinus. The degree of vaccuolation and necrosis were positively related with the concentration of 2MTHF.

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Salamat, Negin, and Mehdi Zarie. "Fish histopathology as a tool for use in marine environment monitoring: a review." Comparative Clinical Pathology 25, no. 6 (November 18, 2014): 1273–78. http://dx.doi.org/10.1007/s00580-014-2037-0.

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Pathak, Renu, D. N. Sadhu*, and Md Noor Alam. "Effects of cartriz on ovarian histopathology of Channa punctatus (Bloch)." International Journal of Bioassays 5, no. 09 (August 31, 2016): 4862. http://dx.doi.org/10.21746/ijbio.2016.09.0014.

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Indiscriminate use of pesticides including cartriz for getting better result in agriculture, has ultimately affected the aquatic biota in general and fishes in particular. The present investigation is aimed to study the adverse effects in the histological make up and gonado somatic index(GSI) of a fresh water fish, Channa punctatus (Bloch) exposed to sublethal concentration of carbamate pesticide, cartriz for different ovarian cycle. The pesticide produced deleterious changes in the ovarian structure and also caused significant changes in GSI, revealing its toxic nature. Parallelly a central set of experiment was kept for observation without any pesticide. Hence it is suggested to use cartriz within prescribed level, to save the environment

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Ventura, Arlene Sobrinho, Márcia Mayumi Ishikawa, Andrea Maria de Araújo Gabriel, Helcy Lylian Nogueira Silbiger, Fabiana Cavichiolo, and Ricardo Massato Takemoto. "Histopathology from liver of tuvira ( Gymnotus spp.) parasitized by larvae of nematodes." Ciência Rural 46, no. 7 (April 5, 2016): 1233–39. http://dx.doi.org/10.1590/0103-8478cr20150881.

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ABSTRACT: The aim of this study was to evaluate the histological changes in the liver of thirty-five Gymnotusspp. parasitized by endohelminths collected between April 2012 to October 2013 in commercial bait fish farming of Pantanal basin. Histological cuts of 7µm were stained with hematoxylin-eosin for parasites research and liver changes and have also been submitted to the Perls histochemical method for evaluation of hemosiderosis (Fe+++) based on the incidence degree and severity of change (Grade I, II and III) and tests for the presence of central melanomacrophages. Parasites identified were: Brevimulticaecumsp. with a prevalence of 22,9%, Eustrongylidessp 17,1%, Contracaecumtype I 68,7%, Contracaecumtype II 5,7%, Contracaecumtype III 5,7% and larvae of Anisakidae11,4%. Histological analysis showed intense disorganization of hepatic parenchyma with degenerate hepatocytes due to high parasitic infection, changes that can be deleterious and compromise the organism functioning, being harmful to the health of evaluated animals. Also evidencing normal tissue interleaved with different stages of Fe+++ deposit in grades II and III, injuring or destroing the cell. Histopathological changes in the tuvira's liver suggested a chronic response and the development of a balance relation between tuvira and parasitism by endohelminth identified in this study. There are also a testimony to the health condition of commercial bait fish farming on current ecosystem conditions.

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GIANNOULOPOULOS (Γ.Δ. ΓΙΑΝΝΟΥΛΟΠΟΥΛΟΣ), G. D., R. FARMAKI (Ρ. ΦΑΡΜΑΚΗ), Ch K. KOUTINAS (Χ.Κ. ΚΟΥΤΙΝΑΣ), and A. F. KOUTINAS (Α.Φ. ΚΟΥΤΙΝΑΣ). "Eosinophilic dermatitis with edema (WellsMike syndrome) possibly triggered by cooked fish in a dog." Journal of the Hellenic Veterinary Medical Society 62, no. 4 (November 13, 2017): 320. http://dx.doi.org/10.12681/jhvms.14862.

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A 2-year old intact female mongrel dog was admitted with multifocal skin lesions appearing suddenly and extending symmetrically over most of the body. The dog was living indoors and one day before the admission it had consumed cooked fish. Neither medication, nor vaccination had been given to the dog, at least during the last 3 months. Physical examination revealed only non-pruritic and non-painful macules, papules and plaques that were distributed mainly over the head, pinnae, neck and thorax. The lesions were annular, aeriform or serpiginous with a tendency to coalesce. Skin histopathology (H-E) revealed a superficial dermal edema, post-capillary venule congestion and perivascular to interstitial eosinophilic dermatitis as the main pattern. The dog was initially placed on oral vitamin E, sulphasalazine and doxycycline, but to no avail. As soon as the diagnosis of eosinophilic dermatitis with edema was confirmed by histopathology, the former treatment stopped and oral prednisolone was given for two months during which there was a remarkable improvement of skin lesions and complete disappearance with no relapse. The cooked fish, consumed by the dog the night before the incident, was assumed to be the cause of the acute eosinophilic hypersensitivity reaction.

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Santos, Dércia, Ana Luzio, Ana M. Coimbra, Simone Varandas, António Fontaínhas-Fernandes, and Sandra M. Monteiro. "A Gill Histopathology Study in two Native Fish Species from the Hydrographic Douro Basin." Microscopy and Microanalysis 25, no. 1 (February 2019): 236–43. http://dx.doi.org/10.1017/s1431927618015490.

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AbstractGill histopathology is a valuable tool to evaluate ecosystems health, because the gill is a key site of waterborne pollutant uptake and the first target organ to their toxicity. Consequently, this makes it important in biomonitoring programs. This study aims to evaluate gill histopathological differences in Douro basin native fish species and determine possible associations with water quality and the ecological status classifications. Two native fish species (Pseudochondrostoma duriense and Luciobarbus bocagei) were sampled in four points of the Douro basin: two reference points, Ameixiosa (Paiva River) and Covelas (Bestança River), both classified with an excellent ecological status; and two disturbed points, Castro Daire (Paiva River) and Alvações do Corgo (Corgo River), categorized with a good and a moderate ecological status, respectively. Gill histopathological differences were qualitative and quantitatively analyzed. The histological analysis showed that, in all sampling locations, both species presented some degree of gill differences, such as epithelial lifting, lamellar fusion, and/or necrosis. The histopathological differences evaluation emphasized some variances in the responses between the two species. In nase, the filament and lamellar epithelium proliferation were the histopathological differences that better reflected the river ecological status classification, proving their usefulness in biomonitoring programs.

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Torres-Martínez, Aarón, Alberto J. Sánchez, Nicolás Alvárez-Pliego, Arlette Amalia Hernández-Franyutti, Juan Carlos López-Hernández, and Jesús Bautista-Regil. "GONADAL HISTOPATHOLOGY OF FISH FROM LA PÓLVORA URBAN LAGOON IN THE GRIJALVA BASIN, MEXICO." Revista Internacional de Contaminación Ambiental 33, no. 4 (November 1, 2017): 713–17. http://dx.doi.org/10.20937/rica.2017.33.04.14.

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Schlacher, Thomas A., Julie A. Mondon, and Rod M. Connolly. "Estuarine fish health assessment: Evidence of wastewater impacts based on nitrogen isotopes and histopathology." Marine Pollution Bulletin 54, no. 11 (November 2007): 1762–76. http://dx.doi.org/10.1016/j.marpolbul.2007.07.014.

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Journal articles on the topic 'Fish histopathology'

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