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1

Nguyen, Binh C. "Extraction, purification and application of hydrolysed collagen from fish skin." Journal of Agriculture and Development 17, no. 05 (2018): 114–22. http://dx.doi.org/10.52997/jad.14.05.2018.

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Collagen hydrolysate is a mixture of peptides which have molecular weight less than 20 kDa and are obtained from enzymatic hydrolysis of collagen or gelatin. It has extraordinary properties and bioactivities compared to collagen and gelatin, such as antioxidant, anti-freezing, anti-microbial, ultraviolet ray prevention, and stimulator for the healing hormones of arthritis. Thus, collagen hydrolysate is widely used in functional foods, cosmetics and pharmaceuticals. The procedure to produce hydrolysate collagen from fish skin begins with the process of removing non-collagen substances, then enz
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2

Sæle, Øystein, Andreas Nordgreen, Pål A. Olsvik, Jan I. Hjelle, Torstein Harboe, and Kristin Hamre. "Toxic effects of dietary hydrolysed lipids: an in vivo study on fish larvae." British Journal of Nutrition 109, no. 6 (2012): 1071–81. http://dx.doi.org/10.1017/s000711451200284x.

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We have previously described that fish larvae absorb a larger fraction of dietary monoacylglycerol than TAG. To investigate how dietary hydrolysed lipids affect a vertebrate at early life stages over time, we fed Atlantic cod (Gadus morhua) larvae six diets with different degrees of hydrolysed lipids for 30 d. The different diets had no effect on growth, but there was a positive correlation between the level of hydrolysed lipids in the diets and mortality. Important genes in lipid metabolism, such as PPAR, farnesoid X receptor (FXR) and stearoyl-CoA desaturase (SCD), were regulated by the diff
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3

Li, Wan Jun, Shun Sheng Chen, and Wei Qiang Qiu. "Comparative Analysis of Amino Acid Composition in Antarctic Krill and White Shrimp." Advanced Materials Research 941-944 (June 2014): 1114–19. http://dx.doi.org/10.4028/www.scientific.net/amr.941-944.1114.

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The purpose of this experiment is to evaluate the nutritional value of amino acids and its commercial value in the fish processing sector in Antarctic krill with cross-referenced in the white shrimp. For determination of the composition of 17 amino acids in the two shrimp muscle, free amino acids were measured using sulfosalicylic acid method, hydrolysed amino acid using hydrochloric acid hydrolysis.17 kinds of hydrolysed amino acids are measured in the muscle of Antarctic krill and White shrimp, which total content is 513.59±18.56mg/g and 537.61±16.8mg/g respectively.16 kinds of free amino ac
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4

Auwal, Shehu Muhammad, Mohammad Zarei, Azizah Abdul-Hamid, and Nazamid Saari. "Response Surface Optimisation for the Production of Antioxidant Hydrolysates from Stone Fish Protein Using Bromelain." Evidence-Based Complementary and Alternative Medicine 2017 (2017): 1–10. http://dx.doi.org/10.1155/2017/4765463.

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Protein hydrolysates produced from different food sources exhibit therapeutic potential and can be used in the management of chronic diseases. This study was targeted to optimise the conditions for the hydrolysis of stone fish protein to produce antioxidant hydrolysates using central composite design (CCD) by response surface methodology (RSM). The stone fish protein was hydrolysed under the optimum predicted conditions defined by pH (6.5), temperature (54°C), E/S ratio (1.5%), and hydrolysis time (360 min). The hydrolysates were then evaluated for 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•)
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Wang, Shao Yun, Jun Zhao, Zhi Bin Xu, and Jin Hong Wu. "Preparation, Partial Isolation of Antifreeze Peptides from Fish Gelatin with Hypothermia Protection Activity." Applied Mechanics and Materials 140 (November 2011): 411–15. http://dx.doi.org/10.4028/www.scientific.net/amm.140.411.

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Antifreeze proteins have characteristics of inhibiting the growth of crystals, decreasing the injury of cells and can retain the structure, texture and quality of productions. The purpose of this study is to obtain natural antifreeze peptides, and to investigate the hypothermia protection activity on bacteria. Gelatin derived from shark skin was hydrolysed to obtain antifreeze peptides. The most appropriate protease and hydrolysis time was selected with the index of the hypothermia protection activity on bacteria. The hydrolysate was subsequently added on to Sephadex G-50 gel filtration column
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6

Cirne, D. G., P. Bond, S. Pratt, P. Lant, and D. J. Batstone. "Microbial community analysis during continuous fermentation of thermally hydrolysed waste activated sludge." Water Science and Technology 65, no. 1 (2012): 7–14. http://dx.doi.org/10.2166/wst.2011.705.

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Acidogenic fermentation of thermally hydrolysed waste activated sludge was carried out at laboratory scale in two reactors operated under different hydraulic retention times (HRT). Process performance was assessed in terms of volatile fatty acid (VFA) composition and yield. The diversity of the microbial population was investigated by constructing a 16S rRNA gene library and subsequent phylogenetic analysis of clones. Fluorescence in situ hybridization (FISH) was used to assess the relative abundance of different bacterial groups. Bacteroidetes and Firmicutes were the dominant taxonomic groups
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7

Adegbenjo, A. A., O. O. Oluwatosin, O. A. Osinowo, et al. "Response of exotic turkey (Meleagris gallopavo) poults to diets substituted with varying levels of hydrolysed feather meal or blood meal as partial replacement for fish meal." Nigerian Journal of Animal Production 47, no. 2 (2020): 100–106. http://dx.doi.org/10.51791/njap.v47i2.107.

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Recent concerns over the seasonal availability and high cost of conventional protein sources has necessitated the search for alternative feed protein with lesser feed-food competitive properties yet desirable and appropriate to poults. A study was designed to determine the response of exotic turkey poults (Meleagris gallopavo) to diets substituted with varying levels of hydrolysed feather meal (HFM) or blood meal (BLM) as partial replacement for fishmeal for 112days. One hundred and ninety-two British United Turkeys BUT) were allotted to eight dietary treatments comprising four replicates and
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8

León-López, Arely, Lucía Fuentes-Jiménez, Alma Delia Hernández-Fuentes, Rafael G. Campos-Montiel, and Gabriel Aguirre-Álvarez. "Hydrolysed Collagen from Sheepskins as a Source of Functional Peptides with Antioxidant Activity." International Journal of Molecular Sciences 20, no. 16 (2019): 3931. http://dx.doi.org/10.3390/ijms20163931.

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The extraction and enzymatic hydrolysis of collagen from sheepskins at different times of hydrolysis (0, 10, 15, 20, 30 min, 1, 2, 3 and 4 h) were investigated in terms of amino acid content (hydroxyproline), isoelectric point, molecular weight (Mw) by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) method, viscosity, Fourier-transform infrared (FTIR) spectroscopy, antioxidant capacity by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays, thermal properties (Differential Scanning Calorimetry) and morphology by s
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9

Bransden, M. P., C. G. Carter, and B. F. Nowak. "Effects of dietary protein source on growth, immune function, blood chemistry and disease resistance of Atlantic salmon (Salmo salar L.) parr." Animal Science 73, no. 1 (2001): 105–13. http://dx.doi.org/10.1017/s1357729800058100.

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AbstractMany studies with fin fish have demonstrated the potential to use alternative dietary protein sources to fish meal based on growth responses, although these trials mostly neglect to determine if such protein sources affect immune function. This study investigated the effect of fish meal replacement with dehulled lupin meal (LPN) or hydrolysed poultry feather meal (FTH). Atlantic salmon (Salmo salar L.) parr were supplied isonitrogenous and isoenergetic diets with 40% of the dietary protein provided by LPN or FTH, or 400 g/kg of the dietary protein provided equally by LPN and FTH (MIX).
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10

Hoffmann, Lilianna, Mateusz Rawski, Silvia Nogales-Merida, and Jan Mazurkiewicz. "Dietary Inclusion of Tenebrio Molitor Meal in Sea Trout Larvae Rearing: Effects on Fish Growth Performance, Survival, Condition, and GIT and Liver Enzymatic Activity." Annals of Animal Science 20, no. 2 (2020): 579–98. http://dx.doi.org/10.2478/aoas-2020-0002.

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AbstractSea trout (Salmo trutta m. trutta) is a species for which effective methods of rearing are still being developed. They need high-quality protein in their diet, but, considering the ecological consequences of fishmeal production, new sources of protein are needed. Presently, insect meal is one of the most promising alternative sources of protein in the diets of farm animals. Insect production does not result in excessive gas emissions, waste generation or the risk of obtaining low-quality dietary protein. The study on sea trout larvae was carried out for 60 days. Four diets were given t
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11

Drotningsvik, Aslaug, Svein A. Mjøs, Daniela M. Pampanin, et al. "Dietary fish protein hydrolysates containing bioactive motifs affect serum and adipose tissue fatty acid compositions, serum lipids, postprandial glucose regulation and growth in obese Zucker fa/fa rats." British Journal of Nutrition 116, no. 8 (2016): 1336–45. http://dx.doi.org/10.1017/s0007114516003548.

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AbstractThe world’s fisheries and aquaculture industries produce vast amounts of protein-containing by-products that can be enzymatically hydrolysed to smaller peptides and possibly be used as additives to functional foods and nutraceuticals targeted for patients with obesity-related metabolic disorders. To investigate the effects of fish protein hydrolysates on markers of metabolic disorders, obese Zucker fa/fa rats consumed diets with 75 % of protein from casein/whey (CAS) and 25 % from herring (HER) or salmon (SAL) protein hydrolysate from rest raw material, or 100 % protein from CAS for 4
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12

Bui, Thi Thu Hien, Thanh Binh Nguyen, Thi Diem Pham, et al. "Research on formula establishment to produce nutritional seasoning powder from pangasius by-product hydrolysed protein." Ministry of Science and Technology, Vietnam 63, no. 6 (2021): 50–56. http://dx.doi.org/10.31276/vjst.63(6).50-56.

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The processing of pangasius fish has produced a number of by-products with economic and biological value such as heads, bones, and fins, but these products have not been fully utilised. The hydrolysed protein powder from pangasius by-products has a high nutritional content, attractive taste and mainly used as the raw material in the production of some value-added food products. The purpose of this study was to develop a formula to create seasoning products from protein powder hydrolysed pangasius by-products. The main ingredients such as pangasius protein powder, starch, basic spices (salt, su
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13

Beirão, Luiz Henrique, Ian Mckintoch Mackie, Evanilda Teixeira, and César Damian. "Purification and characterisation of trypsin-like enzyme from the pyloric caeca of cod (Gadus morhua) II." Brazilian Archives of Biology and Technology 44, no. 1 (2001): 33–40. http://dx.doi.org/10.1590/s1516-89132001000100005.

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A trypsin -like enzyme from the pyloric caeca of cod (Gadus morhua) was purified by affinity chromatography on CHOM Sepharose 4B. Some characteristics were established by its catalytic activity on T.A.M.E., typical enzyme substrate, and serine protease inhibitors. The enzyme had an isoelectric point of 5.30 and 5.89 and was very similar in amino acid composition to bovine trypsin, but differed in having a higher relative amount of acidic amino acids and a lower amount of basic amino acids. The enzyme also hydrolysed fish protein substrates.
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14

Choudhury, Gour S., and Akhilesh Gautam. "Effects of hydrolysed fish muscle on intermediate process variables during twin-screw extrusion of rice flour." LWT - Food Science and Technology 36, no. 7 (2003): 667–78. http://dx.doi.org/10.1016/s0023-6438(03)00087-2.

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15

Xing, Peichuan, Dan Liu, Wen-Gong Yu, and Xinzhi Lu. "Molecular characterization of an endo-type chitosanase from the fish pathogenRenibacteriumsp. QD1." Journal of the Marine Biological Association of the United Kingdom 94, no. 4 (2014): 681–86. http://dx.doi.org/10.1017/s0025315413001859.

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Renibacteriumsp. QD1, a bacteria strain capable of hydrolysing chitosan, was isolated from the homogenate of small crabs. An extracellular chitosanase, Csn-A, was purified from the QD1 fermentation broth. The enzyme was purified to homogeneity, with a yield of eight-fold, 67% recovery and a specific activity of 1575 U/mg proteins. The molecular weight of Csn-A was estimated to be 26.1 kDa by SDS-PAGE. Unlike other chitosanases, the purified Csn-A displayed maximal activity at a pH range of 5.3–6.5, and it was stable in a broad pH range of 5.0–10.0. The optimum temperature for chitosanlytic act
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16

PARAMÁ, A., R. IGLESIAS, M. F. ÁLVAREZ, J. LEIRO, F. M. UBEIRA, and M. L. SANMARTÍN. "Cysteine proteinase activities in the fish pathogenPhilasterides dicentrarchi(Ciliophora: Scuticociliatida)." Parasitology 128, no. 5 (2004): 541–48. http://dx.doi.org/10.1017/s0031182004004883.

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This study investigated protease activities in a crude extract andin vitroexcretion/secretion (E/S) products ofPhilasterides dicentrarchi, a ciliate fish parasite causing economically significant losses in aquaculture. Gelatin/SDS–PAGE analysis (pH 4, reducing conditions) detected 7 bands with gelatinolytic activity (approximate molecular weights 30–63 kDa) in the crude extract. The banding pattern observed in analysis of E/S products was practically identical, except for 1 low-molecular-weight band detected in the crude extract but not in the E/S products. In assays with synthetic peptidep-ni
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17

Folco, E. J. E., L. Busconi, C. B. Martone, and J. J. Sánchez. "Fish skeletal muscle contains a novel serine proteinase with an unusual subunit composition." Biochemical Journal 263, no. 2 (1989): 471–75. http://dx.doi.org/10.1042/bj2630471.

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Proteinase I, an enzyme previously shown to be able to degrade contractile and cytoskeletal elements of white-croaker (Micropogon opercularis) myofibrils, was purified to apparent homogeneity by chromatography on DEAE-Sephacel, octyl-Sepharose CL 4B and arginine-Sepharose 4B. Its Mr was determined to be 269,000 by Sephacryl S-300 gel filtration. Under denaturing conditions, the enzyme dissociated into two subunits with Mr 20,000 and 15,500, in a molar ratio of 1.8:1. Proteinase I showed a pH optimum of 8.5. The enzyme was strongly inhibited by several serine proteinase inhibitors, whereas inhi
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18

Alvarez, M. J., A. Díez, C. López-Bote, M. Gallego, and J. M. Bautista. "Short-term modulation of lipogenesis by macronutrients in rainbow trout (Oncorhynchus mykiss) hepatocytes." British Journal of Nutrition 84, no. 5 (2000): 619–28. http://dx.doi.org/10.1017/s0007114500001951.

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Rainbow trout (Oncorhynchus mykiss) hepatocytes were cultured under simulated conditions of varying nutritional status to explore the short-term modulation by dietary substrates of the main lipogenic enzymes: glucose-6-phosphate dehydrogenase (G6PD), malic enzyme (ME), ATP-citrate lyase (ACL), acetyl-CoA carboxylase (ACoAC) and fatty acid synthetase (FAS). Primary cultures were individually exposed to varying amounts of glucose, hydrolysed casein and long-chain polyunsaturated fatty acids (PUFA) for 12 h. A second set of experiments was designed to evaluate the effects of mixing different rela
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19

Espe, M., K. Ruohonen, and A. El-Mowafi. "Hydrolysed fish protein concentrate (FPC) reduces viscera mass in Atlantic salmon (Salmo salar) fed plant-protein-based diets." Aquaculture Nutrition 18, no. 6 (2012): 599–609. http://dx.doi.org/10.1111/j.1365-2095.2012.00944.x.

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20

Martínez-Llorens, Silvia, Stefano Peruzzi, Inger-Britt Falk-Petersen, et al. "Digestive tract morphology and enzyme activities of juvenile diploid and triploid Atlantic salmon (Salmo salar) fed fishmeal-based diets with or without fish protein hydrolysates." PLOS ONE 16, no. 1 (2021): e0245216. http://dx.doi.org/10.1371/journal.pone.0245216.

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Triploid, sterile Atlantic salmon (Salmo salar) could make a contribution to the development of the farming industry, but uncertainties about the performance and welfare of triploids have limited their adoption by farmers. In this study, we compared the ontogeny of digestive tract morphology and enzyme activities (pepsin, trypsin, chymotrypsin, alkaline phosphatase and aminopeptidase) of diploid and triploid Atlantic salmon. Fish were fed diets based on fishmeal (STD) or a mix of fishmeal and hydrolysed fish proteins (HFM) whilst being reared at low temperature from start-feeding to completion
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21

SUNYER, J. Oriol, Lluis TORT, and John D. LAMBRIS. "Diversity of the third form of complement, C3, in fish: functional characterization of five forms of C3 in the diploid fish Sparus aurata." Biochemical Journal 326, no. 3 (1997): 877–81. http://dx.doi.org/10.1042/bj3260877.

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We have recently shown that Sparus aurata, the gilthead sea bream (a diploid species), similarly to rainbow trout (a quasi-tetraploid species), possesses multiple forms of the third form of complement (C3). In the present study we have evaluated the ability of the gilthead sea bream proteins to function as active C3 molecules. All five C3 isoforms could be fixed covalently to sheep erythrocyte ghosts and were able to bind to various complement-activating surfaces in the presence of MgEGTA. In the absence of MgEGTA their binding capacities generally increased, presumably as a result of classica
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22

Ren, J., H. Wang, M. Zhao, Ch Cui, and X. Hu. "Enzymatic hydrolysis of grass carp myofibrillar protein and antioxidant properties of hydrolysates." Czech Journal of Food Sciences 28, No. 6 (2010): 475–84. http://dx.doi.org/10.17221/343/2009-cjfs.

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Myofibrillar protein was extracted from grass carp, a freshwater fish, and hydrolysed using five commercial proteases (papain, pancreatin 6.0, bromelain, Neutrase 1.5MG, and Alcalase 2.4 L). The antioxidant activities of the hydrolysates were determined. Pancreatin 6.0 proved to be the most efficient protease for hydrolysing myofibrillar protein with a very high protein recovery (90.20%), its hydrolysates exhibiting the highest hydroxyl radical (•OH) scavenging activity (IC<sub>50</sub> = 349.89 ± 11.50 μg/ml) out of all five hydrolysates. Molecular weigh
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23

Che Razali, HasnorIzzati, Ismail Zainol, Wan Rusmawati Wan Mahamod, et al. "Effect of Fish Scale Hydrolysed Collagen on Stability of Water-in-Virgin Coconut Oil Emulsion for Potential Topical Application." Journal of Engineering and Applied Sciences 14, no. 20 (2019): 7647–55. http://dx.doi.org/10.36478/jeasci.2019.7647.7655.

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24

Çankırılıgil, Ekrem Cem, Nermin Berik, and Esen Alp Erbay. "Optimization of hydrolyzation procedure for amino acid analysis in fish meat with HPLC-DAD by Response Surface Methodology (RSM)." Ege Journal of Fisheries and Aquatic Sciences 37, no. 2 (2020): 113–23. http://dx.doi.org/10.12714/egejfas.37.2.01.

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In this research, with the aim of maximizing amino acid content by different hydrolization procedures of fish meat were carried out and analysed with high performance liquid chromatography (HPLC) equipped with diode array detector (DAD). Response surface methodology (RSM) was used to determine factors that used in the experiments. The RSM suggested 16 different hydrolization trials between the ranges of normality as 3 N - 8 N, temperature as 90°C-110°C and duration as 12-24 hours were run. In the following, hydrolysed samples were analysed with HPLC-DAD and obtained data were evaluated with Bo
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25

Harnedy-Rothwell, Pádraigín A., Neda Khatib, Shaun Sharkey, et al. "Physicochemical, Nutritional and In Vitro Antidiabetic Characterisation of Blue Whiting (Micromesistiuspoutassou) Protein Hydrolysates." Marine Drugs 19, no. 7 (2021): 383. http://dx.doi.org/10.3390/md19070383.

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Protein hydrolysates from low-value underutilised fish species are potential sources of high-quality dietary protein and health enhancing peptides. Six blue whiting soluble protein hydrolysates (BW-SPH-A_F), generated at industrial scale using different hydrolysis conditions, were assessed in terms of their protein equivalent content, amino acid profile and score and physicochemical properties in addition to their ability to inhibit dipeptidyl peptidase IV (DPP-IV) and stimulate the secretion of insulin from BRIN-BD11 cells. Furthermore, the effect of simulated gastrointestinal digestion (SGID
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26

Hu, Xiao, Mouming Zhao, Laihao Li, et al. "Emulsifying Properties of Cross-Linking Between Proteins Extracted from Cold/Hot Pressed Peanut Meal and Hydrolysed Fish (Decapterus Maruadsi) Proteins." International Journal of Food Properties 17, no. 8 (2014): 1750–62. http://dx.doi.org/10.1080/10942912.2012.724755.

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27

Martosuyono, Pujoyuwono, Yusro Nuri Fawzya, Gintung Patantis, and Sugiyono Sugiyono. "Enzymatic Production of Fish Protein Hydrolysates in A Pilot Plant Scale." Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology 14, no. 2 (2019): 85. http://dx.doi.org/10.15578/squalen.v14i2.398.

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Protease enzyme produced from Bacillus sp was employed to hydrolyze fish protein hydrolysates (FPH) under controlled conditions at a batch-pilot plant scale-process. Thirty kilograms of fish meat was mincedand mixed with 60 liters of water in 100 liters stainless steel vessel and 20,000 units of protease enzyme was added per kg of fish. Hydrolysis of fish was carried out at 55 oC for 6 hours. Multi stage of filtration were done to separate the FPH from unhydrolized fish residue. Mass balance were carried out to determine the rate of hydrolysis and yields. W ithout pH adjustment, 80% of substra
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28

Uczay, Juliano, Eduardo Kelm Battisti, Rafael Lazzari, et al. "Fish meal replaced by hydrolysed soybean meal in diets increases growth and improves the antioxidant defense system of silver catfish ( Rhamdia quelen )." Aquaculture Research 50, no. 5 (2019): 1438–47. http://dx.doi.org/10.1111/are.14019.

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29

Belchior, Silvia G. Estevao, and Gabriela Vacca. "Fish protein hydrolysis by a psychrotrophic marine bacterium isolated from the gut of hake (Merluccius hubbsi)." Canadian Journal of Microbiology 52, no. 12 (2006): 1266–71. http://dx.doi.org/10.1139/w06-083.

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The aims of this study were to identify a psychrotrophic bacterium, strain CR41, producing a cold adapted protease during growth at low temperatures and to evaluate the ability of the cells to hydrolyze hake fish protein. The strain was isolated from the intestinal tract of hake collected from the San Jorge Gulf (Patagonia, Argentina) and it was identified as Pseudoalteromonas. Growth and fish protein hydrolysis were determined using an aerated simple mineral medium plus 10% fish protein concentrate. Proteolytic activity was measured at 7 and 22 °C during culture in the concentrate. Protease p
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YABU, Takeshi, Shuji KISHI, Toshiro OKAZAKI, and Michiaki YAMASHITA. "Characterization of zebrafish caspase-3 and induction of apoptosis through ceramide generation in fish fathead minnow tailbud cells and zebrafish embryo." Biochemical Journal 360, no. 1 (2001): 39–47. http://dx.doi.org/10.1042/bj3600039.

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Caspase-3 was cloned from zebrafish embryos and its properties were characterized to identify the biological implications of caspase in embryogenesis and apoptosis in zebrafish, which is a model organism in vertebrate developmental biology and genetics. The predicted amino acid sequence, totalling 282 amino acid residues, consisted of the prodomain and large and small subunits. Phylogenetic analysis showed that the cloned zebrafish caspase was a member of the caspase-3 subfamily with approx. 60% identity with caspase-3 from Xenopus, chicken and mammals. In addition, recombinant zebrafish caspa
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31

Chandra, Ranjit Kumar. "Food allergy and nutrition in early life: implications for later health." Proceedings of the Nutrition Society 59, no. 2 (2000): 273–77. http://dx.doi.org/10.1017/s0029665100000306.

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Allergic diseases are a common cause of illness in most industrialized countries. Diet during early childhood is an important determinant of the development of allergy, particularly in high-risk infants who have a parental history of atopy. Maternal avoidance of highly-allergenic foods during pregnancy and lactation, prolonged exclusive breast-feeding, the use of a hydrolysed milk formula, and delayed introduction of dairy products, eggs, fish, nuts and soyabean are associated with a lower incidence of allergic symptoms and signs. These beneficial effects are observed for as long as 18 years o
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32

Fronte, Baldassare, Francesca Abramo, Fabio Brambilla, Mahanama De Zoysa, and Vincenzo Miragliotta. "Effect of hydrolysed fish protein and autolysed yeast as alternative nitrogen sources on gilthead sea bream (Sparus aurata) growth performances and gut morphology." Italian Journal of Animal Science 18, no. 1 (2019): 799–808. http://dx.doi.org/10.1080/1828051x.2019.1581584.

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33

VAZQUEZ, J., S. DOCASAL, M. PRIETO, M. GONZALEZ, and M. MURADO. "Growth and metabolic features of lactic acid bacteria in media with hydrolysed fish viscera. An approach to bio-silage of fishing by-products." Bioresource Technology 99, no. 14 (2008): 6246–57. http://dx.doi.org/10.1016/j.biortech.2007.12.006.

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34

Guilloteau, P., R. Toullec, J. F. Grongnet, P. Patureau-Mirand, J. Prugnaud, and D. Sauvant. "Digestion of milk, fish and soya-bean protein in the preruminant calf: flow of digesta, apparent digestibility at the end of the ileum and amino acid composition of ileal digesta." British Journal of Nutrition 55, no. 3 (1986): 571–92. http://dx.doi.org/10.1079/bjn19860063.

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1. Digesta were collected from eleven preruminant calves fitted with re-entrant (four calves in Expt 1 and three in Expt 2) or single cannulas (four calves in Expt 1) in the terminal ileum. Collection periods lasted 24 h (Expt 1) or 96 h (Expt 2).2. Two milk-substitutes (fish and soya bean) and a control diet were given to the calves. In the control diet, protein was entirely provided by skim-milk powder. In the other two diets, protein was provided mainly by a partially hydrolysed white-fish protein concentrate or a soya-bean protein concentrate prepared by extracting soya-bean meal with hot
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35

Day, O. J., B. R. Howell, and D. A. Jones. "The effect of dietary hydrolysed fish protein concentrate on the survival and growth of juvenile Dover sole, Solea solea (L.), during and after weaning." Aquaculture Research 28, no. 12 (1997): 911–21. http://dx.doi.org/10.1046/j.1365-2109.1997.00922.x.

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Day, O. J., B. R. Howell, and D. A. Jones. "The effect of dietary hydrolysed fish protein concentrate on the survival and growth of juvenile Dover sole, Solea solea (L.), during and after weaning." Aquaculture Research 28, no. 12 (1997): 911–21. http://dx.doi.org/10.1111/j.1365-2109.1997.tb01016.x.

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Noli, Chiara, and Giorgia Beltrando. "The usefulness of a hydrolysed fish and rice starch elimination diet for the diagnosis of adverse food reactions in cats: an open clinical trial." Veterinary Dermatology 32, no. 4 (2021): 326. http://dx.doi.org/10.1111/vde.12970.

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Nilsuwan, Krisana, Kasidate Chantakun, Lalita Chotphruethipong, and Soottawat Benjakul. "Development of Hydrolysis and Defatting Processes for Production of Lowered Fishy Odor Hydrolyzed Collagen from Fatty Skin of Sockeye Salmon (Oncorhynchus nerka)." Foods 10, no. 10 (2021): 2257. http://dx.doi.org/10.3390/foods10102257.

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Lipid oxidation has a negative impact on application and stability of hydrolyzed collagen (HC) powder from fatty fish skin. This study aimed to produce fat-free HC powder from salmon skin via optimization of one-step hydrolysis using mixed proteases (papain and Alcalase) at different levels. Fat removal processes using disk stack centrifugal separator (DSCS) for various cycles and subsequent defatting of HC powder using isopropanol for different cycles were also investigated. One-step hydrolysis by mixed proteases (3% papain and 4% Alcalase) at pH 8 and 60 °C for 240 min provided HC with highe
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Le, Thuy T. M. "Utilization of knife fish bone (Chitala chitala) as a material for fish protein and mineral powder production by enzyme hydrolysis." Journal of Agriculture and Development 18, no. 4 (2019): 33–41. http://dx.doi.org/10.52997/jad.5.04.2019.

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Utilization of knife fish bone as new materials to produce fish protein and mineral powder to increase the value of by-products is necessary. The objective of this study was to investigate effects of Tegalase enzyme concentration, heating time, and drying condition on the quality of hydrolyzed knife fish bone. The results showed that the sample hydrolyzed at 50oC with the Tegalase enzyme concentration of 0.3% resulted in the highest peptide formation and amino acid content in protein solution (3602 peptide bonding and 16.4 g/L, respectively) as well as the highest mineral content (38.8%) in th
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Matricoti, Irina, and Chiara Noli. "An open label clinical trial to evaluate the utility of a hydrolysed fish and rice starch elimination diet for the diagnosis of adverse food reactions in dogs." Veterinary Dermatology 29, no. 5 (2018): 408—e134. http://dx.doi.org/10.1111/vde.12680.

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Lin, Yu-Hsin, Chun-An Chen, Jenn-Shou Tsai, and Guan-Wen Chen. "Preparation and Identification of Novel Antihypertensive Peptides from the In Vitro Gastrointestinal Digestion of Marine Cobia Skin Hydrolysates." Nutrients 11, no. 6 (2019): 1351. http://dx.doi.org/10.3390/nu11061351.

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This research focuses on cobia skin hydrolysates and their antihypertensive effects via the inhibitory activities of angiotensin I-converting enzyme (ACE). Marine fish Cobia (Rachycentron canadum) skin was hydrolysed for 5 h using Protamex and Protease N to obtain the cobia skin protein hydrolysates PX-5 and PN-5, respectively. The soluble protein and peptide contents of the PX-5 were 612 and 270 mg/g, respectively, and for the PN-5, 531 and 400 mg/g, respectively. The IC50 of PX-5 and PN-5 on ACE was 0.221 and 0.291 mg/mL, respectively. Increasing the IC50 from 0.221 to 0.044 mg/mL by simulat
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Mirza, M. Aslam, and E. L. Miller. "In vitro degradability of feed proteins in the rumen: use of non-rumen proteases." Australian Journal of Agricultural Research 56, no. 8 (2005): 797. http://dx.doi.org/10.1071/ar04111.

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Various feed proteins were incubated independently with bacterial protease from Streptomyces griseus (SGP), papain (Corica papaya), and ficin (Ficus glabrata) in a simple laboratory assay to predict ruminal protein degradability. The estimates obtained from in vitro assays were compared with those obtained from an in situ analysis using synthetic fibre bags. The rate and extent of degradation in vitro using proteases from non-rumen sources differed among substrates used. A high correlation coefficient (r2 = 0.99) was observed between N-degradability from the in vitro method using SGP and in si
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Hermaya, Anggi Angelita, Edison Edison, and Andarini Diharmi. "Aktivitas Antioksidan Hidrolisat Protein Ikan Cunang (Congresox talabon)." JURNAL AGROINDUSTRI HALAL 7, no. 1 (2021): 079–86. http://dx.doi.org/10.30997/jah.v7i1.3593.

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Cunang fish (Congresox talabon) has the potential as a raw material for fish protein hydrolyzates. Hydrolysis of cunang fish using the enzyme papain were produced peptides and amino acids that have antioxidant activity. The aim of this study was to determine the antioxidant activity of the protein hydrolyzate of cunang fish using different papain enzyme concentrations. This study used an experimental method with a completely randomized non-factorial design with three replications, with different papain enzyme concentrations (2%, 4%, 6%). The analysis parameters consisted of proximate content a
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Levy, Ryna, and Gene R. Herzberg. "Hydrolysis of long-chain, n-3 fatty acid enriched chylomicrons by cardiac lipoprotein lipase." Canadian Journal of Physiology and Pharmacology 77, no. 10 (1999): 813–18. http://dx.doi.org/10.1139/y99-083.

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The hydrolysis of chylomicrons enriched in long-chain n-3 fatty acids by cardiac lipoprotein lipase was studied. In 60 min, 24.8% of the triacylglycerol fatty acids were released as free fatty acids. The fatty acids were hydrolyzed at different rates. DHA (docosahexaenoic acid, 22:6n-3) and EPA (eicosapentaenoic acid, 20:5n-3) were released at rates significantly less than average. Stearic acid (18:0), 20:1n-9, and alpha-linolenic acid (18:3n-3) were released significantly faster than average. There was no relationship between the rate of release of a fatty acid and the number of carbons or th
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Prastyo, Deny Tri, Wini Trilaksani, and Nurjanah. "Aktivitas Antioksidan Hidrolisat Kolagen Kulit Ikan Nila (Oreochromis niloticus)." Jurnal Pengolahan Hasil Perikanan Indonesia 23, no. 3 (2020): 423–33. http://dx.doi.org/10.17844/jphpi.v23i3.31732.

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Tilapia skin is a by-product of fish processing which can be used as an alternative raw material for collagen and its hydrolyzate. Collagen hydrolyzate is known to have potential biological activities including antioxidants. This study aims to examine the antioxidant activity of collagen hydrolyzate from the skin of tilapia (Oreochromis niloticus) in vitro. This research was divided into three stages including collagen pretratment, collagen extraction and collagen hydrolysis. In the hydrolysis process papain enzymes are used with different concentrations (4,000 U/g, 6,000 U/g, 8,000 U/g sample
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Elavarasan, Krishnamoorthy, and Bangalore Aswathnarayan Shamasundar. "Angiotensin-I-Converting Enzyme Inhibitory Activity and Antioxidant Properties of Cryptides Derived from Natural Actomyosin of Catla catla Using Papain." Journal of Food Quality 2018 (July 8, 2018): 1–8. http://dx.doi.org/10.1155/2018/9354829.

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Natural actomyosin (NAM) from the freshwater fish Catla catla was extracted and hydrolyzed using papain enzyme at different enzyme-to-substrate ratios (0.5%, 1.0%, 2.0%, 5.0%, and 10%) to obtain the cryptides with different degrees of hydrolysis (DH). Derived cryptides were evaluated for bioactive properties such as angiotensin-I-converting enzyme (ACE) inhibitory activity and antioxidant properties. The pattern of hydrolysis of NAM as a function of time revealed that major protein components such as myosin and actin were hydrolyzed within 10 min of hydrolysis. The cryptides obtained with the
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Huang, Chun-Yung, Yung-Hsiang Tsai, Yong-Han Hong, Shu-Ling Hsieh, and Ren-Han Huang. "Characterization and Antioxidant and Angiotensin I-Converting Enzyme (ACE)-Inhibitory Activities of Gelatin Hydrolysates Prepared from Extrusion-Pretreated Milkfish (Chanos chanos) Scale." Marine Drugs 16, no. 10 (2018): 346. http://dx.doi.org/10.3390/md16100346.

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Fish gelatin hydrolysates have been shown to possess various biological activities due to their unique Gly-Pro-Y and Gly-X-Hyp sequences. In the current study, fish gelatin was extracted from non-extruded milkfish scale (FSG1) or extrusion-pretreated milkfish scale (FSG2); extracted gelatins were hydrolyzed with different combinations of Flavourzyme and Alcalase to give four different hydrolysates, namely: FSGH1 (FSG1 hydrolyzed with Flavourzyme), FSGH2 (FSG1 hydrolyzed with Alcalase + Flavourzyme), FSGH3 (FSG2 hydrolyzed with Flavourzyme), and FSGH4 (FSG2 hydrolyzed with Alcalase + Flavourzym
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Szabo, Roman, Sarah Netzel-Arnett, John P. Hobson, Toni M. Antalis, and Thomas H. Bugge. "Matriptase-3 is a novel phylogenetically preserved membrane-anchored serine protease with broad serpin reactivity." Biochemical Journal 390, no. 1 (2005): 231–42. http://dx.doi.org/10.1042/bj20050299.

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We report in the present study the bioinformatic identification, molecular cloning and biological characterization of matriptase-3, a novel membrane-anchored serine protease that is phylogenetically preserved in fish, birds, rodents, canines and primates. The gene encoding matriptase-3 is located on syntenic regions of human chromosome 3q13.2, mouse chromosome 16B5, rat chromosome 11q21 and chicken chromosome 1. Bioinformatic analysis combined with cDNA cloning predicts a functional TTSP (type II transmembrane serine protease) with 31% amino acid identity with both matriptase/MT-SP1 and matrip
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Anggrahini, S., W. Setyaningsih, A. Ningrum, Anto, and N. M. Agustiari. "Physicochemical properties of oci fish joruk (Rastrelliger kanagurta) and the antioxidant activity of joruk hydrolysate." Food Research 4, no. 3 (2020): 786–92. http://dx.doi.org/10.26656/fr.2017.4(3).336.

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Joruk is a traditional fishery product made by spontaneous fermentation process using lactic acid bacteria by salting method, followed by fermentation process. Hydrolysis of proteins with the pepsin and a combination of pepsin and trypsin enzymes by in vitro can produce bioactive peptides. The aims of this study were to investigate the change of soluble protein and lactic acid content in oci fish joruk (Rastrelliger kanagurta) and to determine the influence of the enzyme hydrolysis on the antioxidant activity of joruk. The raw material used in this highest study was the oci fish obtained from
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Lhoste, Evelyne F., Isabelle Catala, Michèle Fiszlewicz, et al. "Influence of caecal microflora and of two dietary protein levels on the adaptation of the exocrine pancreas: comparative study in germ-free and conventional rats." British Journal of Nutrition 75, no. 3 (1996): 433–44. http://dx.doi.org/10.1079/bjn19960145.

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AbstractDietary proteins are degraded by both endogenous enzymes and the caecal microflora. In conventional rats the enzyme content of the pancreas depends on the amount of dietary protein. The influence of the caecal microflora on this process is unknown. We report here the effect of the caecal microflora on pancreatic enzymes (proteases, amylase (EC3.2.1.l), lipase (EC3.1.1.3)) and on colonic metabolites (NH3, urea, short-chain fatty acids). Germ-free and conventional male Fischer rats were fed for 3 weeks with a diet containing 220 or 450 g protein/kg provided as a mixture of fish concentra
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