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1

Arapis, Theodore, and Vincent Reitano. "A Glimmer of Optimism in Government Savings Accumulation? An Empirical Examination of Municipal Unassigned Fund Balance in Florida." Public Finance Review 46, no. 3 (April 21, 2016): 389–420. http://dx.doi.org/10.1177/1091142116643386.

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Few would question that the Great Recession and its aftermath have proved challenging for government financial management. This depressed economic environment has renewed interest in research involving the accumulation and use of the unassigned fund balance. In this study, we use data on Florida cities to examine the factors affecting the unassigned fund balance before, during, and after the Great Recession. According to our findings, building and maintaining savings at high levels have become routine for Florida cities, irrespective of their government form and the economic conditions they face. This research also provides evidence that Florida cities adapt their savings accumulation strategy, depending on the level of unassigned fund balance they are targeting. As a result, Florida cities consider different factors when accumulating unassigned fund balance above, rather than within or below, the minimum range suggested by the Government Finance Officers Association.
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2

Minogue, Patrick, and Seth Wright. "Biology, Control and Invasive Potential of Giant Reed (Arundo donax L.) in Florida." EDIS 2016, no. 2 (April 11, 2016): 5. http://dx.doi.org/10.32473/edis-fr396-2016.

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Arundo donax (L.), also known as giant reed, is a tall, fast-growing, bamboo-like grass that under ideal conditions can reach a height of up to 30 feet and a stem diameter up to 1.5 inches. Giant reed is invasive and difficult to control and has caused economic losses in California, Nevada, Utah, Arizona, New Mexico, and Texas. This species was introduced to Florida over 100 years ago and is currently naturalized in at least 26 of the 67 Florida counties. So far, giant reed has not proved problematic in Florida, but recent permitting of its planting for bioenergy feed stock may increase the risk that it could naturalize into plant communities in Florida and other southeastern states and potentially cause economic losses as well as harm to native species and habitats. This 5-page fact sheet describes the biology of this species and explains some strategies for its control. Written by Pat Minogue and Seth Wright and published by the School of Forest Resources and Conservation, March 2016. FOR328/FR396: Biology, Control and Invasive Potential of Giant Reed (Arundo donax L.) in Florida (ufl.edu)
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3

Djidonou, Desire, Zhifeng Gao, and Xin Zhao. "Economic Analysis of Grafted Tomato Production in Sandy Soils in Northern Florida." HortTechnology 23, no. 5 (October 2013): 613–21. http://dx.doi.org/10.21273/horttech.23.5.613.

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In addition to controlling soilborne diseases, grafting with selected rootstocks has the potential to enhance growth and yields in tomato (Solanum lycopersicum) production. However, information is rather limited regarding its economic viability in different production systems in the United States. The objective of this study was to compare the costs and returns of grafted vs. nongrafted fresh-market tomato production under common management practices in fumigated fields in northern Florida. The field trials were conducted in Live Oak, FL, during Spring 2010 and 2011. ‘Florida 47’ tomato was grafted onto two interspecific hybrid tomato rootstocks: ‘Beaufort’ and ‘Multifort’. Grafted and nongrafted ‘Florida 47’ plants were grown on fumigated raised beds with polyethylene mulch and drip irrigation using recommended commercial production practices for nutrient and pest management. The estimated costs of grafted and nongrafted transplants were $0.67 and $0.15 per plant, respectively, resulting in an additional cost of $3020.16 per acre for using grafted transplants as compared with nongrafted plants. Grafting also led to higher costs of harvesting and marketing tomato fruit as a result of yield improvement (1890 to 2166 25-lb cartons per acre for grafted plant vs. 1457 to 1526 25-lb cartons per acre for nongrafted plant). Partial budget analyses showed that using grafted transplants increased tomato production costs by $4488.03–$5189.76 per acre depending on the rootstock and growing season. However, compared with nongrafted tomato, the net farm return of grafted tomato production was increased by $253.32–$2458.24 per acre based on the tomato shipping point prices. Sensitivity analysis further demonstrated that grafting would be more profitable as the costs of grafted transplants decreased and the market tomato prices increased. These results indicated that although grafting increased the total cost of production, the increase in marketable fruit yield generated significant gross returns to offset costs associated with the use of grafted tomato transplants. Nevertheless, further research is warranted to provide more production budget and net return data about the economic feasibility of grafted tomato production based on a wide range of commercial growing conditions in Florida.
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4

Vonasek, Joseph, and Robert Lee. "Police and Fire Pensions in Florida: A Comparison of Conditions After 10 Years." Compensation & Benefits Review 53, no. 4 (April 5, 2021): 159–74. http://dx.doi.org/10.1177/0886368721999137.

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This article is an analysis of 31 defined benefit police and fire pension plans of 20 municipalities in Florida. The authors conducted a similar assessment of these same plans ten years earlier to determine the fiscal impact of these plans due to state mandates that accompany state funding for each of these plans. The current study analyzes key measures of fiscal health over the last ten years for these same plans to ascertain whether the fiscal condition of these plans remained constant, that is, whether underfunded plans continued to be questionably managed and whether well-funded plans continued to be fiscally stable considering economic trends and the lessening of state mandates on the use of state funding for these plans. The findings show that the overwhelming majority of the plans neither significantly changed their financial condition nor their general ranking among the plans evaluated.
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5

Stanley, Craig D., and Gurpal Toor. "Florida Commercial Horticultural Production: Constraints Limiting Water and Nutrient Use Efficiency." HortTechnology 20, no. 1 (February 2010): 89–93. http://dx.doi.org/10.21273/horttech.20.1.89.

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Application of water and nutrients for horticultural production in Florida can be accomplished through many different methods. Often, the irrigation system that is used determines the potential use efficiency for both water and nutrients. Producers face many constraints, real and perceived, that affect management decisions. This article provides an overview of many of the constraints that are encountered and identifies those management practices that are most effective for overcoming these constraints with respect to commonly used irrigation systems. These constraints include those related to irrigation system design and capabilities, soil factors, cultural practices, management intensity, and economic and regulatory conditions. Ultimately, the goal of this discussion is to determine where future research efforts should be focused and what critical issues may be on the horizon that may affect the Florida horticultural industry.
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6

Singerman, Ariel, Marina Burani-Arouca, and Stephen H. Futch. "The Profitability of New Citrus Plantings in Florida in the Era of Huanglongbing." HortScience 53, no. 11 (November 2018): 1655–63. http://dx.doi.org/10.21273/hortsci13410-18.

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The Florida citrus industry has been enduring the impact of citrus greening since 2005. The disease has been the main driver for the state’s citrus production to plummet by 80% in the past 13 years, causing the industry to downsize drastically. Planting new groves is key to ensuring a supply of fruit for processors and packinghouses to stay in business. However, a key question is whether it makes economic sense to plant a new grove in the current environment. We estimate the establishment and production costs for a new grove under endemic Huanglongbing (HLB; citrus greening) conditions for three different tree planting densities under different market conditions and examine their profitability. Our results show that establishing a new grove with a tree density similar to that of the state’s average is not profitable under current market conditions. However, greater tree densities are profitable despite the greater level of investment required.
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7

Barrett, Charles E., Lincoln Zotarelli, Lucas G. Paranhos, Peter Dittmar, Clyde W. Fraisse, and John VanSickle. "Economic Feasibility of Converting from a Bare Ground System with Seepage Irrigation to Plasticulture for Cabbage Production: Where is the Risk?" HortScience 53, no. 6 (June 2018): 875–81. http://dx.doi.org/10.21273/hortsci12966-18.

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Weather has a major influence on cabbage (Brassica oleracea var. capitata L.) production. Variation in yield between years and cropping seasons is common in North America. Cabbage in Florida has historically been cultivated on bare ground with seepage irrigation. The objectives of this study were to compare yield and profit of a bare ground cabbage production system used in Florida with an alternative plasticulture system. Data from various cabbage trials were combined by production system and used to create regression equations that predicted yield based on air temperature and solar radiation that were significantly correlated with yield. The regression equations were then simulated with correlated stochastic air temperature and solar radiation to estimate the yield distributions for both systems. Cabbage price ($/Mg fresh) was stochastically simulated (correlated to yield) to be used in the profit model. The profit model was created by using the product of yield and the price per unit yield minus fixed and variable costs associated with production and marketing. Simulated profit for bare ground and plasticulture was used to estimate their respective distributions to provide a tool for making better management decisions in the presence of risky weather conditions. The plasticulture system was estimated to have a 36% higher cost but a 57% higher profit than the bare ground system. This is, in large part, because the simulated mean yield for the bare ground system was 29.7 Mg·ha−1 compared with 54.4 Mg·ha−1 for plasticulture. These findings confirmed that plasticulture is an economically viable best management practice for cabbage production in Northeast Florida.
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8

Morera, Maria C., Paul F. Monaghan, Michael D. Dukes, Ondine Wells, and Stacia L. Davis. "Evaluating Florida Homeowner Response to Smart Irrigation Controllers." HortTechnology 25, no. 4 (August 2015): 511–21. http://dx.doi.org/10.21273/horttech.25.4.511.

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Smart irrigation controllers are capable of substantially decreasing landscape water applications under residential high water-use conditions in Florida. Their implementation has been incentivized by governmental agencies and water utilities in an effort to reduce public-supply water demand and conserve water resources. However, the bulk of the research on smart controllers for urban landscapes has focused on performance dimensions. To successfully promote them, feedback from end-users is critical. This paper provides an evaluation of homeowner response to evapotranspiration (ET)-based and soil moisture sensor (SMS)-based smart controllers installed as part of a pilot project conducted in Orange County, FL. The objectives of the study were to collect demographic information, assess conservation attitudes and irrigation system knowledge, and gather feedback on the use of smart controllers from the pilot project’s residential cooperators. Data were collected through an online survey and analyzed using relative frequency distributions, text analysis, independent means t tests, and logistic regression. Results indicated that a majority of survey participants were satisfied with their controllers and planned to continue using them. Both ET and SMS controllers were consistently praised for saving money and irrigating efficiently. However, the likelihood that participants would continue using their controllers after the completion of the project was only significantly predicted by their levels of technical knowledge regarding the workings of the devices and whether they had experienced any challenges operating them. Efforts to promote both initial and long-term adoption may be most effective by emphasizing the economic benefits of investing in smart irrigation controllers and by disseminating best management practices that facilitate their understanding and successful operation.
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9

Lu, Huangjun, Alan L. Wright, and David Sui. "Responses of Lettuce Cultivars to Insect Pests in Southern Florida." HortTechnology 21, no. 6 (December 2011): 773–78. http://dx.doi.org/10.21273/horttech.21.6.773.

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Banded cucumber beetle [BCB (Diabrotica balteata)], serpentine leafminer [SL (Liriomyza trifolii)], and aphids (Homoptera: Aphididae) are among the major insect pests that cause significant economic damage to lettuce (Lactuca sativa) in southern Florida. Four romaine cultivars and three iceberg cultivars, currently used in Florida's lettuce production, were evaluated from Oct. 2010 to Jan. 2011 in separate field experiments for their responses to insect infestation. Lettuce cultivars differed significantly in their responses to infestations of BCB and aphids. Cultivar 70096 had the lowest percent (3.7%) of BCB feeding damage among the romaine cultivars. Romaine cultivar Manatee also had significantly lower BCB feeding damage (12.1%) than the susceptible cultivars Okeechobee (19.8%) and Terrapin (19.1%). The lowest level of infestation of aphids was observed on ‘Manatee’, followed by ‘70096’, whereas ‘Okeechobee’ and ‘Terrapin’ had severe infestation of aphids. The iceberg cultivars were similar to one another in their responses to BCB and SL but not in their response to aphid infestation. Yield was decreased from 3% to 37% for six of the seven cultivars grown under the adverse environmental conditions of insect infestations and cold weather in Dec. 2010, but the yield of ‘70096’ did not decline. The cultivars identified as resistant to insects can play an important role in integrated pest management (IPM), which may decrease pesticide application, reduce production costs, and protect the environment.
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10

Oliver-Smith, Anthony. "Hurricanes, Climate Change, and the Social Construction of Risk." International Journal of Mass Emergencies & Disasters 38, no. 1 (March 2020): 1–12. http://dx.doi.org/10.1177/028072702003800101.

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The advent of climate change is now increasing awareness of the potential of the intensifying peril and frequency of hurricanes for island and coastal dwellers. Considering current demographic data on migration, residence and development, the climatological findings regarding hurricane frequency, intensity, precipitation, and size become particularly menacing. However, despite the intensification and frequency of hurricanes, recognition of the social construction of risk and disasters requires that greater attention be paid to the social and economic drivers of the conditions of exposure and vulnerability that characterize coastal and island communities. The intersection of increasingly intense and frequent hurricanes associated with climate change is discussed in the context of patterns of social, demographic and economic change in the state of Florida in the United States which is located on many of the major paths of hurricanes generated in the Atlantic basin. The paper concludes with an assessment of the role that anthropologists must play in research, practice and policy making reducing the risk of disasters related to hurricane impact.
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11

Ivana Grace Sofia Radja and Leo Riski Sunjaya. "Pembangunan Ekowisata Melalui Konsep Ekonomi Kreatif Berbasis Seni dan Budaya." Kajian Ekonomi dan Akuntansi Terapan 1, no. 2 (June 11, 2024): 217–23. http://dx.doi.org/10.61132/keat.v1i2.202.

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In modern times, economic development has become the most important part of increasing economic growth in Indonesia, through the creative economy. New breakthroughs are needed in the field of ecotourism based on arts and culture. This research was conducted in the colorful village of Kalilo in Banyuwangi, where environmental conditions that are economically and socially unfavorable can transform these deficiencies into potential for sustainable development. By maximizing aspects of talent, tolerance and technology as well as instilling education, especially nationalist education, in the development of arts and culture in the village. The method used is descriptive qualitative based on Richard Florida's theory with the 3T concept (Talent, Tolerance, Technology). The results of the research that has been carried out have found that the use of the 3T concept from Richard Florida is able to dissect several aspects such as the condition of the people of Kalilo Village which is compact with a high level of creativity and is able to improve the economy of the surrounding community.
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12

Núñez-Elisea, Roberto, and Jonathan H. Crane. "Canopy Manipulation to Enhance Early Season Cropping of `Arkin' Carambola in South Florida." HortScience 33, no. 3 (June 1998): 548d—548. http://dx.doi.org/10.21273/hortsci.33.3.548d.

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Carambola (Averrhoa carambola L.) is a fruit tree that exhibits nearly year-round flowering and fruiting in its natural, tropical habitat. The carambola industry of subtropical south Florida is based almost exclusively on the local cultivar Arkin. `Arkin' is susceptible to harsh winter conditions, which debilitate canopy growth and limit the harvest season to about 7 months (July to February). Crop value increases up to eight-fold during times of scarce production. Less than 10% of total crop volume is produced when fruit value is highest. Strategies to enhance early season cropping of `Arkin' carambola were evaluated during a 3-year period. Under favorable growth conditions, carambola trees readily initiate flowers throughout most of the canopy. Pruning 3- to 4-year-old branches to their main axes (branch length was reduced to about one-half of original size) during early spring caused abundant and synchronous flower initiation on pruned branches, and produced a crop in late June-early July. Fruit from pruned branches had excellent appearance due to lack of wind damage. Fruit from intact portions of the canopy began to be harvested 3 to 4 weeks later. In 9-year-old trees, the fall crop was manually shaken off the tree in November-December, when most fruits were about 50% of their final size. Crop removal resulted in abundant flowering (more than three-fold compared to trees which retained their fruit until harvest) throughout the canopy during early spring and also produced an early crop ((48 kg/tree) in early July. Carambola growers in south Florida have begun to adopt selective pruning and fall crop sacrifice to increase economic returns of their groves.
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13

Shi, Lijia, Jinghui Wang, Zhifeng Gao, Xin Zhao, Francesco Di Gioia, Haichao Guo, Jason Hong, Monica Ozores-Hampton, and Erin Rosskopf. "Economic Analysis of Anaerobic Soil Disinfestation for Open-field Fresh-market Tomato Production in Southwest and North Florida." HortTechnology 29, no. 6 (December 2019): 777–87. http://dx.doi.org/10.21273/horttech04332-19.

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With the phase-out of methyl bromide due to its impact on ozone depletion, research has focused on developing alternative chemical and biologically based soil disinfestation methods. Anaerobic soil disinfestation (ASD) was developed to control plant-parasitic nematodes, weeds, and soilborne pathogens. However, whether farmers will adopt ASD methods on a large scale is unknown. This study evaluates the economic viability of using ASD in open-field, fresh-market tomato (Solanum lycopersicum) production, drawing on data from field experiments conducted in 2015 in Immokalee, FL, and Citra, FL. The experiment included three treatments: chemical soil fumigation (CSF), ASD1 [the standard ASD treatment with 1482 gal/acre molasses and 9 tons/acre composted poultry litter (CPL)], and ASD0.5 (the reduced rate ASD treatment with 741 gal/acre molasses and 4.5 tons/acre CPL). Results from the economic analysis show that ASD treatments require higher labor costs than CSF regarding land preparation and treatment application. However, yields from ASD treatments are higher than those resulting from CSF, and the improvement in yield was enough to offset the increased labor costs. Relative to CSF, ASD0.5, and ASD1 achieved additional net returns of $630.38/acre and $2770.13/acre, respectively, in Immokalee, FL. However, due to unexpected conditions unrelated to soil treatments, the net return of ASD1 was lower than that of CSF in Citra, FL. Breakeven analysis indicates that ASD treatments would remain favorable even with an increase in the molasses price. However, when the tomato price is low, ASD could potentially lose its advantage over CSF.
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Boston, P. Qasimah, M. Miaisha Mitchell, Kourtney Collum, and Clarence C. Gravlee. "Community Engagement and Health Equity." Practicing Anthropology 37, no. 4 (September 1, 2015): 28–32. http://dx.doi.org/10.17730/0888-4552-37.4.28.

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The Health Equity Alliance of Tallahassee (HEAT) is a diverse team of academic and community members interested in examining existing health disparities and in improving the health of those experiencing social or economic disadvantage. In 2010, HEAT designed a Heart Health study to examine the causes of high blood pressure and heart disease among African Americans living in Tallahassee, Florida. The study aimed to understand how the social environment, neighborhood conditions, social relationships, experience of stress, and the stress of racism impacts health. This paper discusses the community engagement plan used to involve Tallahassee residents in a dialogue and conversation about the HEAT Heart Health (HHH) Study. Using the example of HHH, we argue for a community engagement approach that involves community residents in all processes of a research study, including the reporting of findings.
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15

LEOGRANDE, WILLIAM M. "Enemies Evermore: US Policy Towards Cuba After Helms-Burton." Journal of Latin American Studies 29, no. 1 (February 1997): 211–21. http://dx.doi.org/10.1017/s0022216x96004683.

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When Cuban MiGs blasted two civilian planes out of the sky over the Straits of Florida on February 24 1996, they also destroyed any chance of improved relations between Cuba and the United States as long as Fidel Castro remains in power. In Washington, outrage over the shoot-down resurrected the Helms-Burton bill, the most punitive legislation on Cuba since the early 1960s. On 12 March, President Bill Clinton signed the bill into law as the Cuban Liberty and Democratic Solidarity Act of 1996. In addition to assorted sanctions aimed at foreigners doing business in Cuba, the most consequential provision incorporates the US economic embargo into law. Heretofore, the embargo was based on presidential executive orders; it could be tightened or loosened at the president's discretion as conditions warranted. Under Helms-Burton, no president can lift or even relax the embargo until Fidel Castro and the existing Cuban regime fall from power. At a time when Cuba's domestic social and economic system is changing at break-neck speed, Washington's 35-year-old policy of hostility has just been chiselled in stone.
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16

Sahliyeh, Emile. "MARION BOULBY, The Muslim Brotherhood and the Kings of Jordan, 1945–1993, South Florida–Rochester–Saint Louis Studies on Religion and the Social Order, vol. 18 (Atlanta: Scholars Press for the University of South Florida, 1999). Pp. 188. $44.50 cloth." International Journal of Middle East Studies 32, no. 3 (August 2000): 425–26. http://dx.doi.org/10.1017/s0020743800002609.

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In this book, Marion Boulby traces the rise and evolution of the Muslim Brotherhood movement in Jordan. In chapter 1, she gives a brief historical survey of Jordan's state-building and the economic and social developments in the country between 1921 and 1989. In chapter 2, she describes the formative phase of the Brotherhood and the conditions surrounding its establishment between 1945 and 1957. Here, Boulby contends that the concern over the future of Palestine rather than competition with leftist groups was the primary motive behind the formation of the Brotherhood. She also highlights the conservative nature of the founders of the movement and their adoption of a reformist ideology and pragmatic political stands. In her opinion, the norms of political conservatism, reform, and pragmatism, which have characterized the movement throughout its history, were behind the forging of a close alliance between the Brothers and the monarchy.
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17

Meagher, Robert L., Rodney N. Nagoshi, James T. Brown, Shelby J. Fleischer, John K. Westbrook, and Carlene A. Chase. "Flowering of the Cover Crop Sunn Hemp, Crotalaria juncea L." HortScience 52, no. 7 (July 2017): 986–90. http://dx.doi.org/10.21273/hortsci11981-17.

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Sunn hemp, Crotalaria juncea L., is a warm-season legume that is planted before or after a vegetable cash crop to add nutrients and organic matter to the soil, for weed-growth prevention, and to suppress nematode populations. Sunn hemp flowers may also provide nectar and pollen for pollinators and enhance biological control by furnishing habitat for natural enemies. Despite these benefits, adoption in the United States has been limited because of restricted availability of seeds, particularly in temperate climates. Experiments were conducted in north-central Florida to compare flowering and seed production of domestic and foreign sunn hemp lines across different seeding rates and planting dates. Our objectives were to test whether a low seeding rate would result in the production of higher numbers of flowers and to test whether planting earlier in the season would also result in higher numbers of flowers. Our results over a 2 year period showed that the domestic cultivar AU Golden is capable of substantial flowering and seed production in the test region, confirming the compatibility of local environmental conditions. Seed costs suggest that ‘AU Golden’ is comparable with sunn hemp lines grown in foreign countries and is much less expensive than the standard cultivar Tropic Sun from Hawaii. The results demonstrate the potential economic viability of early flowering cultivars of sunn hemp as a cover crop alternative in Florida to improve soils in agricultural landscapes.
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Giri, Chandra, Jordan Long, and Prapti Poudel. "Mangrove Forest Cover Change in the Conterminous United States from 1980–2020." Remote Sensing 15, no. 20 (October 18, 2023): 5018. http://dx.doi.org/10.3390/rs15205018.

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Mangrove forests in developed and developing countries are experiencing substantial transformations driven by natural and anthropogenic factors. This study focuses on the conterminous United States, including Florida, Texas, and Louisiana, where coastal development, urbanization, hydrological pattern alterations, global warming, sea level rise, and natural disasters such as hurricanes contribute to mangrove forest changes. Using time-series Landsat data and image-processing techniques in a cloud computing platform, we analyzed the dynamics of mangrove forests every five years from 1980 to 2020. Each thematic product was independently derived using a region of interest (ROI) suitable for local conditions. The analysis was performed using consistent data sources and a unified classification methodology. Our results revealed that the total mangrove area in the conterminous United States (CONUS) in 2020 was 266,179 ha. with 98.0% of the mangrove area in Florida, 0.6% in Louisiana, and 1.4% in Texas. Approximately 85% of the CONUS mangrove area was found between 24.5° and 26.0° latitude. Overall, mangrove forests in the CONUS increased by 13.5% from 1980 to 2020. However, the quinquennial variation in aerial coverage fluctuated substantially. The validation of 2020 using a statistical sample of reference data confirmed the high accuracy of 95%. Our results can aid policymakers and conservationists in developing targeted strategies for preserving the ecological and socio-economic value of mangrove forests in the conterminous United States. Additionally, all the datasets generated from this study have been released to the public.
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K. Dhanalakshmi, K. Chitra, S. Dharani S. Gowshika, Jagadeesh Kumar C. Lavanya, and V. Ambethgar. "Comparative Analysis of Temperature on Yield of Different Varieties of Oyster Mushroom Production." International Journal of Current Microbiology and Applied Sciences 10, no. 10 (October 10, 2021): 296–301. http://dx.doi.org/10.20546/ijcmas.2021.1010.036.

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Oyster mushrooms are economical and most easily grown of all cultivated edible mushrooms. The crop has a range of varieties, differing in form, colour, texture and odor, which can be cultivated throughout the year under a diverse agro-climatic conditions. Three different oyster mushroom species viz., Hypsizygus ulmarius (var. CO2), Pleurotus eous (var. APK1) and Pleurotus florida (var. PF) along with three cropping rooms of varied temperatures was used for the study. Among the different cropping rooms, thatched shed with a temperature of 23o C recorded a highest yield of 748g, 712 and 673 g per 500 g of substrate by PF, CO 2 and APK 1 respectively than AC room and Concrete room. The temperature of the cropping room is inversely proportional to the yield of oyster mushroom. Hence, the thatched shed was best suited for oyster mushroom cultivation, which was both economic and easy to use.
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Puri, Atul, Gregory E. MacDonald, William T. Haller, and Megh Singh. "Growth and Reproductive Physiology of Fluridone-Susceptible and -resistant Hydrilla (Hydrilla verticillata) Biotypes." Weed Science 55, no. 5 (October 2007): 441–45. http://dx.doi.org/10.1614/ws-07-020.1.

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Hydrilla is one of the most serious aquatic weed problems in the United States, and fluridone is the only U.S. Environment Protection Agency (USEPA)–approved herbicide that provides relatively long-term systemic control. Recently, hydrilla biotypes with varying levels of fluridone resistance have been documented in Florida. Several biotypes of hydrilla varying in resistance levels were maintained in 950-L tanks under ambient sunlight and day-length conditions from September 2004 to September 2005 in absence of fluridone. Phenotypic measurements were performed during this 1-yr period to monitor differences in growth and reproductive physiology. All fluridone-resistant biotypes (except R3) were growing at the same rate or greater than the susceptible hydrilla. These data suggested that there are no deleterious effects on growth and reproductive physiology because of development of fluridone resistance. Aggressive spread of fluridone-resistant dioecious hydrilla in aquatic ecosystems can severely affect hydrilla management and, consequently, cause substantial and long-lasting ecological and economic problems throughout the southern United States.
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McCabe, Brian E., Emma M. Mitchell, Rosa Maria Gonzalez-Guarda, Nilda Peragallo, and Victoria B. Mitrani. "Transnational Motherhood: Health of Hispanic Mothers in the United States Who Are Separated From Children." Journal of Transcultural Nursing 28, no. 3 (April 19, 2016): 243–50. http://dx.doi.org/10.1177/1043659616644960.

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Immigration often results in the separation of families, and in particular transnational parenting, which is the separation of mothers from children. Transnational mothers may have greater risks for poor mental health and behavioral conditions such as substance abuse, violence, sexual risk, and depression. This study was a secondary analysis of self-reported data from 425 Hispanic mothers (328 with no separations, 60 separated from an adult child, and 37 separated from a minor child) enrolled in a randomized trial of a sexual health group intervention in South Florida (USA). Separations were related to mother’s age, years in the United States, family income, number of people living on income, acculturation to the United States, occupational/economic stress, immigration stress, and lifetime exposure to abuse. A follow-up analysis described the types of childhood or adulthood abuse experienced by mothers with separations. These findings provide new information for nurses about the experience of immigrant mothers.
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Ferrarezi, Rhuanito S., Arun D. Jani, H. Thomas James, Cristina Gil, Mark A. Ritenour, and Alan L. Wright. "Sweet Orange Orchard Architecture Design, Fertilizer, and Irrigation Management Strategies under Huanglongbing-endemic Conditions in the Indian River Citrus District." HortScience 55, no. 12 (December 12, 2020): 2028–36. http://dx.doi.org/10.21273/hortsci15390-20.

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The prevalence of Huanglongbing (HLB) in Florida has forced growers to search for new management strategies to optimize fruit yield in young orchards and enable earlier economic returns given the likelihood of HLB-induced yield reductions during later years. There has been considerable interest in modifying orchard architecture design and fertilizer and irrigation management practices as strategies for increasing profitability. Our objectives were to evaluate how different combinations of horticultural practices including tree density, fertilization methods, and irrigation systems affect growth, foliar nutrient content, fruit yield, and fruit quality of young ‘Valencia’ sweet orange [Citrus sinensis (L.) Osbeck] trees during the early years of production under HLB-endemic conditions. The study was conducted in Fort Pierce, FL, from 2014 to 2020 on a 1- to 7-year-old orchard and evaluated the following treatments: standard tree density (358 trees/ha) and controlled-release fertilizer with microsprinkler irrigation (STD_dry_MS), high tree density (955 trees/ha) with fertigation and microsprinkler irrigation (HDS_fert_MS), and high tree density with fertigation and double-line drip irrigation (HDS_fert_DD). Annual foliar nutrient concentrations were usually within or higher than the recommended ranges throughout the study, with a tendency for decreases in several nutrients over time regardless of treatment, suggesting all fertilization strategies adequately met the tree nutrient demand. During fruit-bearing years, canopy volume, on a per-tree basis, was higher under STD_dry_MS (6.2–7.2 m3) than HDS_fert_MS (4.3–5.3 m3) or HDS_fert_DD (4.9–5.9 m3); however, high tree density resulted in greater canopy volume on an area basis, which explained the 86% to 300% increase in fruit yield per ha that resulted in moving from standard to high tree density. Although fruit yields per ha were generally greatest under HDS_fert_MS and HDS_fert_DD, they were lower than the 10-year Florida state average (26.5 Mg·ha−1) for standard tree density orchards, possibly due to the HLB incidence and the rootstock chosen. Although tree growth parameters and foliar nutrient concentrations varied in response to treatments, management practices that included high tree density and fertigation irrespective of irrigation systems produced the highest fruit yields and highest yield of solids. Soluble solids content (SSC) and titratable acidity (TA) were lower, and the SSC-to-TA ratio was highest under STD_dry_MS in 2016–17, with no treatment effects on quality parameters detected in other years. Both drip and microsprinkler fertigation methods sufficiently met tree nutrient demand at high tree density, but additional research is needed to determine optimal fertilization rates and better rootstock cultivars in young high-density sweet orange orchards under HLB-endemic conditions in the Indian River Citrus District.
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Kloudová, Jitka, and Ondřej Chwaszcz. "An Analysis of the Creative Potential in Individual Regions of The Czech Republic." Creative and Knowledge Society 3, no. 1 (July 1, 2013): 17–27. http://dx.doi.org/10.2478/v10212-011-0028-y.

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Abstract Purpose of the article: Although the economic growth and society are two independent terms at the first sight, they are in fact closely connected and interact with each other. The main topic of this work is the creative economy, which is considered to be a part of growth theories. Thanks to the new approach, theorists supplement these theories with the demographic and the socio-cultural factor. First, the work establishes a comprehensive theoretical framework for economic growth (linked with creativity). Furthermore, it analyses the representation of creativity in the regions of the Czech Republic. Methodology/methods: The primary aim of this work is to carry out an analysis that charts the representation of the creative potential in the regions of the Czech Republic. The analysis uses the new creative index, which was created by modifying of the Richard Florida´s creative index. Consequently, the work uses the new creative index within the framework of smaller regional units as opposed to national groupings. Additional advantages of this model include the number and measurability of the indexes. Scientific aim: The aim of this work is not just demonstrating the dependence between the presence of creative people and economic growth, but in addition, the work aims to seek out the factors that influence the allocation of creative employees. Findings: The work demonstrates high mutual dependence between creativity and economic growth with the help of the correlation coefficient. The assumptions that creative regions are more attractive on the social and knowledge basis were also confirmed. Conclusions (limits,implications etc): Territorial units must consequently work to develop conditions, which support the establishment and the development of the creative environment in order to retain their competitiveness and support the economic growth.
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Shukla, Asmita, Sanjay Shukla, and Alan W. Hodges. "Recovering Nitrogen from Farm-Scale Drainage: Mechanism and Economics." Transactions of the ASABE 61, no. 1 (2018): 197–206. http://dx.doi.org/10.13031/trans.12277.

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Abstract. A two-year study evaluated the nitrogen (N) fluxes, processing, and treatment efficiency (TE) of a 14.85 ha stormwater detention area (SDA) receiving drainage from a vegetable farm in subtropical Florida. The TE was 62% and 89% in years 1 and 2, respectively. Seepage N losses are often ignored in estimating stormwater treatment. Approximately 11% and 20% of the incoming N left the SDA through seepage, reducing the TE to 51% and 67% in years 1 and 2, respectively, indicating the importance of subsurface N losses for downstream water quality. Rainfall variability controlled the timing and volume of the inflow drainage and surface water levels inside the SDA. Variable water levels controlled the aerobic and anoxic conditions inside the SDA, thus controlling the N processing and treatment. Coupled nitrification-denitrification, as a result of frequent wetting-drying cycles, was the main N treatment pathway during year 1. Drought conditions in year 2 led to 89% less surface outflow compared to year 1, resulting in water volume retention being the main process for N retention. The N TE could be increased from 68% to 86% if about two-thirds (63%) of aboveground biomass in the SDA area is harvested annually during the dry season. A payment for environmental services (PES) framework, with the state as buyer and the SDA owner as seller of N treatment services, was evaluated with a 20-year net present worth (NPW) of biomass harvesting for enhanced N treatment. The economic analysis included the benefit from composting the harvested biomass for on-farm use. A positive NPW ($835,000) indicated the economic feasibility of the project, predicting an annual benefit of $42,000 year-1 for the 112 ha farm. Scale-up of the PES approach can offer additional N treatment and C sequestration services as well as increased farm productivity. Keywords: Best management practices, Impoundments, Nutrient treatment, Payment for environmental services, Phytoremediation, Stormwater.
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De Vito, Donald. "Unleashing the Entrepreneurial Spirit: Nurturing Elementary Students' Potential for Innovation, Creativity, and Fearless Exploration." International Journal of Music Entrepreneurship and Leadership 1, no. 1 (July 15, 2023): 17. http://dx.doi.org/10.61629/ijmel.v1i1.9.

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This paper advocates for the teaching of entrepreneurship to elementary students, specifically those from 98 percent minority, low socio-economic (Title I schools in the US) by recognizing and harnessing their inherent entrepreneurial spirit. The prevailing notion that entrepreneurship is exclusively reserved for adults disregards the natural inclination of young learners to explore, create, innovate, and fearlessly try new things. By fostering an environment that encourages curiosity, creativity, and problem-solving, educators can tap into the entrepreneurial potential of elementary students. This philosophical position emphasizes the importance of instilling an entrepreneurial mindset in students, promoting a growth mindset, integrating real-world connections, and empowering students to take ownership of their learning. Through age-appropriate activities and authentic experiences, students develop essential entrepreneurial skills and qualities such as critical thinking, adaptability, resilience, and a sense of agency. By embracing this perspective, educators can cultivate a generation of entrepreneurial thinkers and doers who are equipped with the skills, mindset, and creativity necessary for future success in the 21st century. Imagine the following scenario of events that took place in the Rawlings Elementary approach utilized in this paper. A collaboration with the Haitian Center for Inclusive Education founded by Gertrude Bien Aime, in which Rawlings students shared local neighborhood riffs and rhythms they bring into the school each day with neighborhood music of the children in Port au Prince. The 40 students with special needs at the Haiti inclusion school are raised by Gertrude at the Notre Maison Orphanage where she maintains their care and safety regardless of outside conditions. When the Rawlings students met the students and Gertrude the female students in the class requested repeated conversations with Gertrude as a role model but also because of her entrepreneurial ability to face the challenges of gangs, food shortages, and variety of needs the students with disabilities she rescued and raises they engage with. The first comment from my students when the Zoom session ended was “Fundraiser!”. Disregarding their own daily socio economic, educational and potential safety needs in their lives and focusing on the opportunity to assist others. One student had suggesting recording their original music arrangements of their neighborhood music on Spotify. By incorporating authentic experiences, such as connecting with local entrepreneurs, engaging in community projects, and exploring entrepreneurship in various fields, students can grasp the practical applications of entrepreneurial skills. As a result of these shared experiences, the Society for Education, Music and Psychological Research (SEMPRE) with support from Dr. Graham Welch paid for Gertrude to fly to Florida to attend the Florida Council for Exceptional Children Conference to receive added training and entrepreneurial opportunities. Bien Aime received the Florida Council for Exceptional Children’s Landis Stetler Award for service to the field of special education and will be hired and brought to the US this fall as a consultant for the Florida Atlantic University Center for Autism and Related Disabilities. The purpose of this consultancy will be to encourage the parents of children with special needs in the Haitian community there to overcome a perceived stigma in receiving services for their children at the center. The funding also will help her in her efforts to continue to support the students in Haiti. This Rawlings Elementary approach helps students understand how their ideas and actions can make a meaningful impact on their community and the world around them and through journaling, critique discussions and critical thinking on these activities they take steps to improve their reading and writing skills.
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Zhang, S., Y. Fu, Z. Mersha, X. Mo, and A. J. Palmateer. "First Report of a Leaf Spot on Basella alba Caused by a Bipolaris sp. in Florida." Plant Disease 95, no. 7 (July 2011): 880. http://dx.doi.org/10.1094/pdis-04-11-0276.

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Malabar spinach (Basella alba L.) is a fast-growing, perennial vegetable crop grown largely in the tropics of Asia and Africa. This crop is widely used in the cuisine of different regions for its thick, semisucculent leaves, mild flavor, and mucilaginous texture. Leaf spots were observed on both surfaces of symptomatic leaf samples received from a home garden in Homestead, FL in November 2009. The necrotic lesions (up to 2 mm in diameter) were round, semicircular, or irregular-shaped with grayish centers surrounded by dark brown borders. A fungus was consistently isolated from symptomatic tissues on clarified V8 (CV8) agar. Fungal colonies on CV8 agar were black and velvet-like with minimal mycelial growth and conidiophores were dark brown, simple, borne singly or in groups upon the substrate. Conidia were straight, pale to medium golden brown, rounded at the ends with three to six septa, and on average measured 75 × 15 μm (48 to 97 × 9 to 18 μm). Cultural and conidial characteristics of the isolates were closely similar to those of a Bipolaris sp. (1). The internal transcribed spacer (ITS) region (~570 bp) of rDNA was amplified using the primers ITS1/ITS4 and sequenced bidirectionally (GenBank Accession No. JF506092). Subsequent database searches by the BLASTN program indicated that the resulting sequence had a 95% identity over 531 bp with the corresponding gene sequence of Bipolaris portulacae (GenBank Accession No. AY004778.1), a fungal pathogen reported to cause leaf spot on purslane (Portulaca oleracea) (2,3). However, our isolate has consistently smaller conidia and does not match descriptions of B. portulacae (BPI 871173, U.S. National Fungus Collections). The pathogenicity was confirmed through inoculation of healthy Malabar spinach plants with conidia of the isolate reproduced on CV8. Six Malabar spinach plants were inoculated with a suspension containing 1 × 106 conidia per ml and sprayed until runoff (approximately 15 ml per plant) with a handheld pressurized canister. Another six noninoculated plants served as a control. Immediately after inoculation, plants were covered with plastic bags for 24 h to maintain high relative humidity and maintained in a greenhouse under ambient conditions. Ten days after inoculation, the symptoms described above were observed on leaves of all inoculated plants, whereas symptoms did not develop on the control plants. A Bipolaris sp. was reisolated and identified by the above methods, fulfilling Koch's postulates. This pathogenicity test was carried out three times. To our knowledge, this is the first report of a Bipolaris sp. affecting Malabar spinach in Florida. Further work should be conducted to confirm identity of these isolates. Because of limited plantings of Malabar spinach, the economic importance of this disease in Florida is currently not known. Nevertheless, this pathogen poses a threat to the growing market of continuously produced oriental vegetables in Florida. References: (1) J. L. Alcorn. Mycotaxon 39:361, 1990. (2) S. A. Alfieri, Jr. et al. Bull. 14. Index of Plant Diseases in Florida (Revised). Florida Dep. Agric. Consumer Serv., Div. Plant Ind., 1984. (3) D. F. Farr and A. Y. Rossman. Fungal Databases. Systematic Mycology and Microbiology Laboratory. ARS, USDA. Retrieved from http://nt.ars-grin.gov/fungaldatabases/ , 25 January 2010.
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27

Rayamajhi, M. B., P. D. Pratt, T. D. Center, and G. S. Wheeler. "Differential Response by Melaleuca quinquenervia Trees to Attack by the Rust Fungus Puccinia psidii in Florida." Plant Disease 94, no. 9 (September 2010): 1165. http://dx.doi.org/10.1094/pdis-94-9-1165b.

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Melaleuca quinquenervia (melaleuca) is an exotic invasive tree in Florida, Hawaii, and some Caribbean islands (1,2). Puccinia psidii (rust fungus) attacks melaleuca as well as other plants in a few genera of the Myrtaceae and Heteropyxidaceae, both members of the Myrtales (1,2). Disease occurs on succulent stems and foliage of melaleuca, causing twig dieback and defoliation (3). Melaleuca trees growing under similar field conditions exhibit susceptible or resistant reactions toward this fungus. To document this differential susceptibility of melaleuca to P. psidii, we visually evaluated 331 field-grown melaleuca trees from southeast Florida for occurrence of disease attributes: pustules (susceptible), nonpersistent halos (resistant), or asymptomatic (no macroscopic symptoms) conditions on leaves and succulent twigs during February and March when symptoms were at their peak. Percentages of trees manifesting susceptible, resistant, and asymptomatic responses to this fungus were 85.8, 13.0, and 1.2%, respectively. A screenhouse study was conducted to corroborate these observations by raising plants from composite seed sources and maintaining them in seven 3.8-liter plastic pots that were filled with commercial potting media. Nine to eleven plants per pot (with new foliage) were individually tagged, grown to 30 to 45 cm high, and spray inoculated (during February and March) with uredospores (~2 × 106/ml) obtained from melaleuca trees and suspended in water. Inoculated plants were placed on a screenhouse bench under infected trees and subjected to additional inoculum, thereby simulating field conditions. Evaluations made weekly during a 4-week period revealed that susceptible, resistant, and asymptomatic seedlings constituted 63.3, 33.6, and 3.2%, respectively, of the tagged plants. To assess the stability of these fungal and host attributes over time and space, we multiplied two P. psidii susceptible and two resistant plants from cuttings. We spray inoculated 6 to 13 rooted cuttings from each plant types with uredospores (0.8 to 2 × 106/ml) obtained from diseased melaleuca trees and suspended in water. These plants were incubated in a dew chamber for 72 to 96 h under 100% relative humidity at 19 to 23°C maintained with a 12-h fluorescent light cycle. After incubation, plants were placed randomly on a bench in a screenhouse (21 to 23°C) and evaluated weekly for symptom development during a 4-week experimental period. Noninoculated controls were maintained as well. The experiment was repeated twice. Foliage of the resistant plants developed a few incipient halos whereas 100% of the susceptible plants developed erupted uredinia and were defoliated in both replications. No detectable change in P. psidii virulence and melaleuca susceptibility patterns was observed. Despite wide host range within Myrtales, resistance to P. psidii exists within M. quinquenervia. Other P. psidii susceptible host systems of economic and environmental importance may have host/pathogen relationships similar to that of melaleuca and the selection of resistant individuals from their affected populations may be possible. Additional studies will be needed to ascertain the attributes of virulence or resistance in this rust fungus-melaleuca association. References: (1) M. Glen et al. Australas. Plant Pathol. 36:1, 2007. (2) P. D. Pratt et al. J. Aquat. Plant Manag. 45:8, 2007. (3) M. B. Rayachhetry et al. Biol. Control 22:38, 2001.
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Ajabshir, Sahar, Sarah Stumbar, Innah Lachica, Kevin Gates, Zafar Qureshi, and Fatma Huffman. "Diet-Related Chronic Diseases Among a Multi-Ethnic Group of Uninsured Adults." Current Developments in Nutrition 5, Supplement_2 (June 2021): 95. http://dx.doi.org/10.1093/cdn/nzab035_003.

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Abstract Objectives The prevalence of diet-related chronic diseases, such as obesity, hypertension and type 2 diabetes, among adults in the U.S. is of increasing import during the COVID-19 pandemic. These conditions are among the top risk factors associated with severe COVID-19 morbidity and mortality. The aim of this study was to assess the of diet-related diseases among a multi-racial/ethnic uninsured group in South Florida. Methods Electronic heath records of adult patients (N = 272) from a free clinic in South Florida, a 4-week pre COVID-19 pandemic de-identified dataset was analyzed. Presence of diet-related conditions for each patient was quantified. Numerical and categorical data were compared using independent t-test and Chi-Squared test, respectively. Spearman`s correlation, multi-linear regression, and binary regression models were used to assess the relationship between the variables. Results The sample included females (65%) and males (35%). The mean age was 49.08 ± 14.56 years. There were 26.5% Whites, 33.8% Blacks, 35.6% Asians/Asian-Indians, and 1.1% American Indians. About 24% were Hispanic/Latino. The mean BMI was 29.45 ± 6.76 kg/m² (n = 250). Overall, 87% of patients had at least one diet-related condition with overweight/obesity being the most observed (75.2%), followed by hypertension (39%), dyslipidemia (27.2%), and diabetes (23.9%). The inter-racial analysis revealed that BMI is a significant predictor of prevalence of hypertension among Whites (P = 0.008) and Blacks (P = 0.002), but not Asians/Asian-Indians (P = 0.536). BMI was a significant predictor of prevalence of dyslipidemia (P = 0.027) and type 2 diabetes (P = 0.006) among Hispanics/Latinos. Bivariate analysis showed a 70.8% co-incidence of hypertension and a 64.4% co-incidence of dyslipidemia among adults with type 2 diabetes. Co-incidence of hypertension among adults with dyslipidemia was at 73%. Conclusions High rate of diet-related chronic diseases was found among the multi-ethnic group of uninsured adults in this study. This supports the need for increased patient-education regarding nutrition in free clinic settings. Nutrition programs tailored for low economic resources and culturally appropriate dietary recommendations could assist uninsured patients with management of their diet-related chronic diseases. Funding Sources This research was supported by an NIH sub-award.
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Iskandar, Mina, Kira M. Ruiz-Houston, Steven D. Bracco, Sami R. Sharkasi, Cecilia L. Calabi Villarroel, Meghna N. Desai, Alexandra G. Gerges, et al. "Deep-Sea Sponges and Corals off the Western Coast of Florida—Intracellular Mechanisms of Action of Bioactive Compounds and Technological Advances Supporting the Drug Discovery Pipeline." Marine Drugs 21, no. 12 (November 28, 2023): 615. http://dx.doi.org/10.3390/md21120615.

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The majority of natural products utilized to treat a diverse array of human conditions and diseases are derived from terrestrial sources. In recent years, marine ecosystems have proven to be a valuable resource of diverse natural products that are generated to defend and support their growth. Such marine sources offer a large opportunity for the identification of novel compounds that may guide the future development of new drugs and therapies. Using the National Oceanic and Atmospheric Administration (NOAA) portal, we explore deep-sea coral and sponge species inhabiting a segment of the U.S. Exclusive Economic Zone, specifically off the western coast of Florida. This area spans ~100,000 km2, containing coral and sponge species at sea depths up to 3000 m. Utilizing PubMed, we uncovered current knowledge on and gaps across a subset of these sessile organisms with regards to their natural products and mechanisms of altering cytoskeleton, protein trafficking, and signaling pathways. Since the exploitation of such marine organisms could disrupt the marine ecosystem leading to supply issues that would limit the quantities of bioactive compounds, we surveyed methods and technological advances that are necessary for sustaining the drug discovery pipeline including in vitro aquaculture systems and preserving our natural ecological community in the future. Collectively, our efforts establish the foundation for supporting future research on the identification of marine-based natural products and their mechanism of action to develop novel drugs and therapies for improving treatment regimens of human conditions and diseases.
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Muñoz, Ed A. "“Why Not Nuevo Mexicano Studies?”: Interrogating Latinidades in the Intermountain West, 1528–2020." Genealogy 5, no. 3 (July 22, 2021): 68. http://dx.doi.org/10.3390/genealogy5030068.

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While there has been an explosion of scholarly interest in the historical and contemporary social, economic, and political status of U.S. Latinx individuals and communities, the majority focuses on traditional Southwestern U.S., Northeastern U.S., and South Florida rural/urban enclaves. Recent “New Destinations” research, however, documents the turn of the 21st century Latinx experiences in non-traditional white/black, and rural/urban Latinx regional enclaves. This socio-historical essay adds to and challenges emerging literature with a nearly five-century old delineation of Latinidad in the Intermountain West, a region often overlooked in the construction of Latina/o identity. Selected interviews from the Spanish-Speaking Peoples in Utah Oral History and Wyoming’s La Cultura Hispanic Heritage Oral History projects shed light on Latinidad and the adoption of Latinx labels in the region during the latter third of the 20th century centering historical context, material conditions, sociodemographic characteristics, and institutional processes in this decision. Findings point to important implications for the future of Latinidad in light of the region’s Latinx renaissance at the turn of the 21st century. The region’s increased Latino proportional presence, ethnic group diversity, and socioeconomic variability poses challenges to the region’s long-established Hispano/Nuevo Mexicano Latinidad.
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31

Ploetz, Randy C., José M. Pérez-Martínez, Edward A. Evans, and Sharon A. Inch. "Toward Fungicidal Management of Laurel Wilt of Avocado." Plant Disease 95, no. 8 (August 2011): 977–82. http://dx.doi.org/10.1094/pdis-08-10-0595.

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Laurel wilt threatens commercial and residential production of avocado (Persea americana) in Florida. Laurel wilt on redbay (P. borbonia) was controlled previously with macroinfusions (injections) of Alamo, an injectable formulation of propiconazole. To determine whether Alamo macroinfusion would be cost effective in commercial avocado production, economic analyses were conducted for various macroinfusion scenarios and a standardized production situation in southern Florida. Under prevailing conditions, macroinfusion was not cost effective. In the interest of identifying alternative means to manage the disease, other fungicides and application measures were evaluated. In all, 20 fungicides in 15 chemical groups and 10 fungicide groups were examined in vitro. In vitro inhibition of the radial growth of the pathogen Raffaelea lauricola was determined on fungicide-amended malt extract agar; demethylation inhibitors (DMIs; fenarimol, myclobutanil, propiconazole, prothioconazole, triadimenol, triadimefon, and triticonazole), quinone outside inhibitors (azoxystrobin, pyraclostrobin, and fluoxastrobin), and a quinone inside inhibitor (fluazinam) had the greatest impact on radial growth (the concentration at which growth was reduced by 50% was ≥0.1 μg ml–1). In greenhouse studies, the most inhibitory products in vitro, plus thiabendazole and two products that were not tested in vitro, flutriafol and a potassium salts mixture of phosphorus acid, were tested for disease suppression on artificially inoculated, potted ‘Simmonds,’ a susceptible avocado cultivar. In general, soil drench applications of the above DMIs and thiabendazole but not azoxystrobin, pyraclostrobin, fluazinam, or the phosphorus acid salt provided significant control of disease (P < 0.05). Topical branch or trunk applications of propiconazole, and triadimenol in 2% Pentrabark, a bark-penetrating surfactant, were also effective at lower rates than were used in drench applications. Comparable levels of disease suppression were achieved when propiconazole was applied at 11% of the rates that were used in soil drenches. Although topical fungicide applications in bark-penetrating surfactants would be a less expensive practice than macroinfusion, moving sufficient concentrations of propiconazole or other fungicides into host xylem will be difficult in trees that are larger than the potted plants that were tested in these trials. Ongoing work examines means by which this goal might be met on fruit-bearing trees in the field.
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Patel, J. S., M. I. Costa de Novaes, and S. Zhang. "First Report of Colletotrichum higginsianum Causing Anthracnose of Arugula (Eruca sativa) in Florida." Plant Disease 98, no. 9 (September 2014): 1269. http://dx.doi.org/10.1094/pdis-09-13-0926-pdn.

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Arugula (Eruca sativa) is grown in Florida and is an important component in packaged salad products. During spring 2013, leaf lesions on arugula caused significant economic losses in Miami-Dade County, Florida. Symptoms initially appeared as small water-soaked lesions that later became circular, sunken, and white in the center with a dark brown to black halo, up to 4 mm in diameter. Acervuli were found under a dissecting microscope on infected leaf lesions with black spines or setae. Occasionally, small, circular, often longitudinal dark brown spots appeared on leaf branches. Leaf tissues (5 × 5 mm) from lesion margins were surface sterilized in 0.9% sodium hypochlorite for 10 min, rinsed with sterile distilled water, and plated on potato dextrose agar (PDA). PDA plates were incubated at 21°C under 24-h fluorescent lights for 4 to 6 days. The fungus initially produced gray mycelium followed by orange conidial mass. Hyphae of the fungus were septate and hyaline. After 5 to 7 days, the fungus produced acervuli with dark brown to black setae (75 to 130 μm long) (n = 20). Conidia were found in the colonies, which were single celled, oblong, hyaline, and 12 to 25 × 4 to 6 μm (n = 20). The cultural and morphological characteristics of the conidia were similar to those for Colletotrichum higginsianum Sacc (1). To further confirm the species of the isolates, the sequence of the ITS region of rDNA, chitin synthase 1 (CHS1), and actin (ACT) was amplified from isolates 05131 and 05132 using primer pairs ITS 1 and ITS 4 (4), CHS-79F and CHS-354R, and ACT-512F and ACT-783R (3), respectively. The sequenced data of each locus were deposited in GenBank with accessions KF550281.1, KF550282.1, KJ159904, KJ159905, KJ159906, and KJ159907. The resulting sequence of ITS showed 100% identity with sequences of C. higginsianum in JQ005760.1, and sequence of ACT gene showed 100% identity with C. higginsianum in JQ005823.1. The sequence of ACT gene and ITS region had ≤99% identity with other closely related Colletotrichum spp. CHS1 gene had 100% identity with JQ005781.1 belonging to C. higginsianum, and one accession JQ005783.1 belonging to C. fuscum. However, ACT gene and ITS region does not share 100% identity with C. fuscum and therefore, sequence data from three loci proves that isolated pathogen is C. higginsianum. All the above mentioned accessions that shared 100% identity with sequences of isolates used in our study have been previously used to represent the species in the C. destructivum clade in a systematics study (2). To confirm its pathogenicity, a suspension of isolate 05132 at 5 × 105 conidia/ml was sprayed on leaves of five arugula plants until runoff. The other five arugula plants sprayed with water served as non-inoculated controls. Both inoculated and non-inoculated plants were separately covered with a plastic bag to maintain high humidity for 24 h at 27 ± 5°C under natural day/night conditions in the greenhouse. Symptoms first appeared 3 to 4 days after inoculation as small water-soaked lesions, which became sunken with dark brown to black margins. Small circular and longitudinal dark brown spots were also seen on leaf branches as seen initially on naturally infected arugula. No symptoms developed on non-inoculated control plants. C. higginsianum was re-isolated from the lesions with the same morphological characteristics as described above, fulfilling Koch's postulates. To our knowledge, this is the first report of C. higginsianum causing anthracnose of arugula in Florida. This pathogen may potentially affect the salad industry in the United States. References: (1) A. J. Caesar et al. Plant Dis. 94:1166, 2010. (2) P. F. Cannon et al. Stud. Mycol. 73:181, 2012. (3) I. Carbone and L. M. Kohn. Mycologia 91:553, 1999. (4) T. J. White et al. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, 1990.
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Olson, S. M., D. O. Chellemi, and P. C. Andersen. "Tomato Little-leaf Syndrome—A Possible Explanation." HortScience 30, no. 4 (July 1995): 767B—767. http://dx.doi.org/10.21273/hortsci.30.4.767b.

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Since the fall of 1986, tomato growers in northwestern Florida and southwestern Georgia production areas have encountered plants in their fields with unusual growth characteristics. Early symptoms consist of interveinal chlorosis of the young leaves. Subsequent top growth becomes severely distorted with leaflets along the midrib failing to expand properly, resulting in a “little-leaf” appearance. Additional symptoms included cessation of terminal growth, leaves with twisted and brittle midribs, and axillary buds failing to develop properly. Fruit that set on mildly affected plants are distorted, with radial cracks extending from the calyx to the blossom scar. In severely affected plants, fruit failed to set. The problem usually occurs at very low levels, but in 2 years since 1986, the problem has caused some economic damage. To determine a possible cause, samples were taken for virus detection. None were detected in affected plants. Samples were also taken of tissue and soil from affected areas for nutrient and pesticide analysis. No explanation could be developed from any of the tissue or soil samples. The problem usually occurs in wet areas and after very warm temperatures. The problem appears to be very similar to a nonparasitic disease that occurs in tobacco, called “frenching.” In tobacco, frenching occurs in wet, poorly aerated soils with a soil pH >6.3 and during warm temperatures. There seems to be an organism or organisms present under certain conditions that live on the root surface and exude chemicals that cause this distorted growth.
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34

Himmelgreen, David, Nancy Romero-Daza, William Alex Webb, Jacquelyn N. Heuer, Deven Gray, and Gabrielle R. Lehigh. "Implementing a Food Prescription Program during COVID-19: Benefits and Barriers." Healthcare 12, no. 2 (January 12, 2024): 182. http://dx.doi.org/10.3390/healthcare12020182.

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Food prescription programs (Food Rx) have the potential to improve management of diet-related chronic diseases or underlying conditions such as type 2 diabetes (T2D), hypertension, and high body mass index (BMI) among food-insecure patients. The purpose of this study was to examine the effectiveness of a Food Rx program implemented in two community-based clinics in Florida. Data were collected through researcher-administered surveys (food insecurity, demographics, and socio-economic variables) and biometric data (HbA1c, blood pressure, and BMI). Key results include the following: (1) Hispanic patients are more likely to utilize the program than their Black and White counterparts (p < 0.001); (2) older patients (≥50 years) have a higher food redemption rate when compared to younger patients (36–49.9 years); (3) food redemption rate is negatively associated with food security scores indicating improvements in food security status over time (r2 = −0.184, p = 0.036); (4) diabetic patients with higher baseline HbA1c (>9%) have significant reductions in glycated hemoglobin (p = 0.011) over time as compared to patients with lower baseline values (<7%); and (5) patients enrolled in the program for at least 6 months have a significant reduction in systolic blood pressure (p = 0.051). Changes in BMI were not significantly associated with redemption rates. This study is significant as it offers insights into the potential benefits and challenges of implementing Food Rx programs to address diet-related chronic diseases among underserved populations.
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Meyering, Bo, Adam Hoeffner, and Ute Albrecht. "Reducing Preharvest Bolting in Open-field-grown Cilantro (Coriandrum sativum L. cv. Santo) through Use of Growth Regulators." HortScience 55, no. 1 (January 2020): 63–70. http://dx.doi.org/10.21273/hortsci14614-19.

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Cilantro (Coriandrum sativum L.), also called coriander, is an herbaceous, annual plant that is cultivated worldwide for its leaves and seeds. Cilantro has a strong propensity to bolt quickly in hot weather and under long-day (LD) conditions, which affects the flavor and renders the crop unmarketable. High incidence of preharvest bolting in open-field production can cause significant economic loss. The phytohormone gibberellic acid (GA) regulates stem elongation and floral initiation in many LD rosette plants. In pilot experiments, we found that GA induced bolting in greenhouse-grown cilantro and that plant growth regulators (PGRs) with anti-GA activity can delay this process. We then explored the effects of different GA inhibitors on reducing the incidence of bolting in cilantro grown in a commercial open-field environment. Four field trials were conducted on a commercial farm near Clewiston in Florida between Fall 2016 and Spring 2018. Different growth regulators were applied at different times, ranging from 5 to 8 weeks after seeding (WAS), and plants were harvested 2 to 3 weeks thereafter. Applications of GA inhibitors significantly reduced the incidence of bolting in three of the four trials, but the extent depended on the type of inhibitor used. The results from one trial were inconclusive due to changes in weather that prevented bolting in the entire field. Overall, plots treated with prohexadione calcium and paclobutrazol were most effective and reduced bolting by up to 78%. Applying the PGRs at 5 and 6 WAS was more effective than at 7 or 8 WAS.
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Tignor, Milton E., John M. Davis, Frederick S. Davies, and Wayne B. Sherman. "Changes in Freezing Tolerance, Water Potential, and Gene Expression of Poncirus trifoliata `Rubidoux' Seedlings Exposed to Acclimating Low Temperatures and Long Days." HortScience 31, no. 4 (August 1996): 580a—580. http://dx.doi.org/10.21273/hortsci.31.4.580a.

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Poncirus trifoliata is a comparatively hardy, cross compatible, and graft compliant relative of Citrus. The citrus industry in Florida has suffered immense economic losses due to freezes. Although much research has been done in citrus freeze hardiness, little work has been on the early induction of freeze tolerance by low temperature. Poncirus trifoliata `Rubidoux' seedlings were germinated in perlite under intermittent mist at about 25°C and natural daylight conditions in a greenhouse and grown 2 weeks. See dlings were then transferred into a growth chamber at 25°C and 16 hour daylength for 1 week. Temperature was lowered to 10°C and tissue samples were collected at 0, 6, 24, and 168 hours. Freezing tolerance, at –6.7°C as determined by electrolyte leakage, and stem (leaves attached) water potential, measured using a pressure bomb, were also recorded for a subset of seedlings for the above intervals. After exposure to low temperature for 48 hours a red coloration became visible at the petiole leaflet junction an d at the buds, with subsequent exposure to low temperature the coloration spread to the leaves. Clones for phenylalanine ammonia lyase (PAL), 4-coumarate:CoA ligase (4CL), and chlorophyll ab binding protein (CAB), and chalcone synthase (CHS) were used to probe RNA isolated from P. trifoliata. PAL and 4CL transcripts increased in response to the low temperature. Significant increases in freeze hardiness occurred within 6 hours in the leaves, and increases continued for up to one week. Water potential increased from –0.6 to –2.0 MPa after 6 hours, then returned to –0.6 MPa after 1 week. These data indicate that increases in freezing tolerance and changes in water potential and gene expression can be detected shortly after low temperature treatments are imposed on P. trifoliata seedlings.
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Phuyal, Dinesh, Thiago Assis Rodrigues Nogueira, Arun D. Jani, Davie M. Kadyampakeni, Kelly T. Morgan, and Rhuanito Soranz Ferrarezi. "‘Ray Ruby’ Grapefruit Affected by Huanglongbing II. Planting Density, Soil, and Foliar Nutrient Management." HortScience 55, no. 9 (September 2020): 1420–32. http://dx.doi.org/10.21273/hortsci15255-20.

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Since the arrival of Huanglongbing (HLB) disease in Florida, several management approaches, including modification of orchard architecture design and nutritional therapy, have been explored. High-density plantings anticipate early economic returns from HLB-affected orchards. With no cure available for HLB, balanced nutrient application through soil and foliar spraying can mitigate the disease. A 2-year study was conducted to investigate the effects of three grapefruit (Citrus paradisi) planting densities [single-row (300 and 440 trees per ha), and double-row high-density (975 trees per ha)], two controlled-release fertilizer (CRF) blends, and foliar-applied micronutrients (FAM) (a blend of B, Mn, and Zn at 0, 1.5, 3, and 6 times the recommended rates) on grapefruit growth and fruit yield, physiological parameters, and foliar nutrient concentrations in an HLB-affected orchard. All the trees tested positive for HLB based on real-time quantitative polymerase chain reaction (qPCR) test. The highest planting density resulted in the lowest trunk diameter and canopy volume. Despite lower yield per tree in 2019–20, 975 trees per ha planting induced the greatest fruit and solid yields per ha. Also, the fruit produced from 975 trees per ha planting tended to be acidic with the deposition of more soluble solids. Use of CRF with higher micronutrients increased canopy volume with the expense of reduced fruit number in 2019–20. FAM did not affect cycle threshold (Ct) value and tree growth parameters. Fruit yield, photosynthesis rate, and stomatal conductance (gS) decreased, and all leaf nutrient concentrations except B increased in 2019–20 with all FAM rates tested. In conclusion, our study showed that high-density planting optimizes yield under HLB-endemic conditions. In addition, supplemental soil and foliar micronutrient application do not enhance yield of HLB-affected trees over a 2-year timeframe, warranting further research for confirmation of results.
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Bonde, M. R., S. E. Nester, D. K. Berner, R. D. Frederick, W. F. Moore, and S. Little. "Comparative Susceptibilities of Legume Species to Infection by Phakopsora pachyrhizi." Plant Disease 92, no. 1 (January 2008): 30–36. http://dx.doi.org/10.1094/pdis-92-1-0030.

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Knowledge of the host range of Phakopsora pachyrhizi is important to agriculture in the United States because of the distinct possibility that economic losses could occur to crops other than soybean. Furthermore, it is possible that alternative hosts could provide a means of overwintering of the pathogen, providing inoculum to initiate epidemics in future years. To clarify the potential importance of soybean rust on nonsoybean legumes and their role in overwintering of the disease, multiple accessions of clover, cowpea, pea, kudzu, lima bean, snap bean, and single accessions of coffee senna, Florida beggarweed, hemp sesbania, hyacinth bean, partridge pea, and showy crotalaria were inoculated under greenhouse conditions with urediniospores of P. pachyrhizi; infected soybean plants served as a control. The four criteria used to assess susceptibility were lesion density, proportion of lesions with sporulating uredinia, average number of uredinia per lesion, and average uredinia diameter, each determined 2 weeks following inoculation. Based on lesion densities, percentage of lesions with sporulation, and average numbers of uredinia per lesion, soybean, kudzu, and pea were the most susceptible species, followed by snap bean. However, because infected pea plants defoliated rapidly, urediniospore production presumably was limited, lessening the potential for epidemics on pea. Cultivars of snap bean produced numerous brown to reddish-brown lesions, many of which sporulated, but numbers of uredinia per lesion were lower than on soybean, kudzu, or pea. The presence of both tan (susceptible) and reddish-brown (resistant) lesions on kudzu demonstrated physiological differentiation on that host. Some kudzu plants appeared to be potentially excellent hosts for overwintering of the disease. The average number of uredinia per lesion appeared to be a valid measurement with which to compare host susceptibilities, and may have epidemiological significance. High susceptibility of a host was characterized by numerous uredinia with a wide range of sizes within individual lesions. In contrast, low susceptibility to rust was characterized by no or a few small uredinia.
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39

Wang, Xiaoheng, and Can Chen. "An empirical analysis of capital assets condition ratio in local governments: the case of Florida counties." Journal of Public Budgeting, Accounting & Financial Management 34, no. 2 (October 29, 2021): 330–46. http://dx.doi.org/10.1108/jpbafm-01-2021-0013.

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PurposeThe main purpose of this paper is to examine the political, economic and institutional determinants of capital assets condition ratio in American local governments using government-wide financial statements.Design/methodology/approachBased on capital assets data from the period of 2011–2016 for the 66 Florida counties as reported on their government-wide financial statements, the authors use a panel two-way fixed effects estimation and a dynamic panel generalized method of moments estimation.FindingsThe authors find that social-economic factors, fiscal capacity and democratic voters explain the capital assets condition ratio in Florida county governments.Research limitations/implicationsThe major findings of this study may only apply to county government in one single state. It may raise the issue of the external validity of our research. It provides policy recommendations for local public officials to maintain and upgrade their capital assets.Originality/valueThe study utilizes a new approach of capital assets condition ratio to measure county government investment in capital assets based on the government-wide financial statements.
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40

Lorier, Estrellita, Maria Stella Zerbino, and Lucia Miguel. "Seasonal Occurrence of Different Grasshoppers Species (Orthoptera: Acridoidea) in Uruguay." International Journal of Biology 8, no. 4 (August 29, 2016): 21. http://dx.doi.org/10.5539/ijb.v8n4p21.

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<p class="1Body">This paper aims at knowing the seasonal occurrence of grasshoppers (Orthoptera: Acridoidea) in Uruguay. The populations of these native herbivores are generally in equilibrium, although population increases were recorded under certain conditions, causing significant economic loss. The population was monitored during five seasons on sites located in the departments of Florida and Durazno. The insects were categorized into young nymphs (I, II and III), older nymphs (IV and V), and adults. At each sampling time, data from the different sites were grouped to calculate the percentage of each category. Twenty-six species were collected, eight of which were abundant. <em>Borellia bruneri </em>(Rehn, 1906), <em>B. pallida </em>(Bruner, 1900), <em>Dichroplus pratensis </em>Bruner, 1900 and <em>D. elongatus </em>Giglio-Tos, 1894 proved to be univoltine. More than one generation of <em>Baeacris pseudopunctulatus </em>(Ronderos, 1964), <em>Orphulella punctata </em>(De Geer, 1773)<em>, Scotussa lemniscata </em>(Stal, 1861) and <em>D. conspersus </em>Bruner, 1900, was recorded per year in some seasons. The first births of <em>B. bruneri</em>, <em>B. pallida</em>, <em>D. pratensis</em>, <em>D. conspersus</em>, <em>S. lemniscata</em> and <em>B. pseudopunctulatus </em>occurred in October, whereas for <em>D. elongatus</em> and <em>O. punctata </em>they happened in November. Considering the eight species, young and older nymphs predominated until early December and from late December to early January, respectively. Adulthood was predominant since the end of January. These results are the basis for establishing a monitoring plan. In order to determine the need for control and to avoid irreparable loss, samples must be intensified from early November to the beginning of December, when the young nymphs are no longer predominant.</p>
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Chyzmar, Ivan, and Volodymyr Hoblyk. "E-sports organizations with franchised networks: formalization of technological and economic development based on optimal operation and upgrade of the hardware." Economic Annals-ХХI 187, no. 1-2 (February 28, 2021): 146–62. http://dx.doi.org/10.21003/ea.v187-15.

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The paper focuses on the priority given to the technological and economic development of e-sports organizations with franchised networks. Attention is paid to the specificity of the process of timely upgrading of e-sports hardware, which involves the upgrading and introduction of new, more sophisticated and advanced gaming and other devices, which determines the number of e-sports disciplines and available e-sports events for the е-sports arena. The success of franchising networks depends on the quantity of the c-sports hardware, which should be similar to the hardware used by the main arena in order to ensure the functionality of a well-structured state-of-the-art training process for e-sport athletes. The purpose of the study is to present a basis for the technological and economic development of e-sports organizations with a franchising network within the formalized system in terms of finding optimal solutions to the problem of hardware upgrades. The study presents a model of technological and economic development of all e-sports organizations with franchised networks as а content area, posing the rules of operation, regulating the process of hardware upgrades and focusing on the sustainability of development. Dynamic programming methods based on Bellman’s equations and the formalization of the problem of hardware replacement through graphic notations, cloud computing in the AnyLogic environment are used to identify and illustrate the features of such solutions. The result of the research is a description of technological development of e-sports organizations with a franchise network with the use of a model that approximates the optimal way of operation and upgrade of related hardware. This development illustrates the optimal way of operation and hardware upgrade of Blizzard Arena and Overwatch League, represented by participants from the United States, the United Kingdom, Germany, France, South Korea and Ukraine. The franchised Overwatch League includes the main arena (Blizzard Arena), as well as Florida Mayhem Club (Miami-Orlando, USA), New York Excelsior Club (New York, USA), London Spitfire Club (London, UK), Vault Club 15 (Kyiv, Ukraine), Immortals Club (Los Angeles, USA), NRG E-sports Club (Berlin, Germany), Misfits Club (Seoul, South Korea), PS4 Training Base (Beijing, China) and Xbox One Training Base (Paris, France). According to the obtained data, the formalization of the technological and economic development of Blizzard Arena suggests a solution to the problem of finding an optimal strategy relevant to optimization of hardware up to the moment of its transfer to the franchised network. Such formalization is highly relevant. They rely on the possible state of the system proposed in our research. That system state identifies the functional Bellman equations. Naturally, emerges a possibility to significantly reduce investment in the e-sports environment of the main arena and the franchise while controlling the quality and functionality of e-sports hardware. The organizations’ focus on two-stage upgrades will reduce investment in major hardware. The study illustrates the formalization of the techno-economic development of the Blizzard Arena through a two-stage upgrade of the Aerocool Advanced Technologies franchise (primary franchise - from producer, secondary franchise - from franchise Blizzard Arena operator). Based on the specific features of the Bellman equations, the development of the Blizzard Arena must take into account the model which determines the feasibility of hardware transferring to a franchised network during the third period of operation, where it operated as long as franchisees enter the maximum profit area. When using the Bellman equations, we suggest that the arena focus on the moment when the function values will correspond to the replacement state of the hardware and the franchise member on the stability of the environment. The formalization of the technical development will make it possible to orient the e-sport organizations with franchised networks to search for conditions sufficient for optimal operation and upgrade of their hardware. As a result, there will appear an optimized system with a content area, which will provide a stable environment for e-sports events at the arena and athletes’ training in franchise clubs.
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42

Darnell, Rebecca L., Jeffrey G. Williamson, Deanna C. Bayo, and Philip F. Harmon. "Impacts of Vaccinium arboreum Rootstocks on Vegetative Growth and Yield in Two Southern Highbush Blueberry Cultivars." HortScience 55, no. 1 (January 2020): 40–45. http://dx.doi.org/10.21273/hortsci14585-19.

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Vaccinium arboreum Marsh is a small tree adapted to low-organic matter soils and is one of the few ericaceous species that tolerates soil pH greater than 6.0. It has a deep root system and is more drought tolerant than cultivated blueberry. The use of V. arboreum as a rootstock for commercial blueberry production has been studied previously in young blueberry plantings. The objective of the current study was to expand on earlier work and evaluate growth, productivity, and tolerance to bacterial leaf scorch (Xylella fastidiosa) in established plantings of own-rooted vs. grafted southern highbush blueberry (SHB). Two field plantings of grafted and own-rooted ‘Meadowlark’ and ‘Farthing’ SHB were established in May 2011: one at the University of Florida–Institute of Food and Agricultural Sciences (UF-IFAS) Plant Science Research and Education Unit in Citra, FL, and the other at a commercial blueberry farm in Archer, FL. At both sites, four rootstock–scion combinations were grown in either pine bark-amended or nonamended soil. Canopy volume was greater in grafted compared with own-rooted ‘Meadowlark’ at both locations throughout the 4 years of the study (2015–18), whereas canopy volume in ‘Farthing’ was not consistently different. For both cultivars and both locations, canopy volume was greater on amended compared with nonamended soil. Although canopy growth was not consistently increased in the grafted compared with own-rooted plants, yield was greater in grafted plants of both cultivars at both locations. Cumulative yield over the 4 years was similar between grafted plants grown on both amended and nonamended soil, and was significantly greater than yield of own-rooted plants on nonamended soil, suggesting the use of this rootstock may decrease the requirement for pine bark amendment. In general, grafted plants produced larger berries, with no negative impacts on fruit soluble solids, titratable acidity, or firmness. ‘Meadowlark’—an SHB cultivar that exhibits high sensitivity to bacterial leaf scorch—displayed decreased development of bacterial leaf scorch symptoms when grafted onto V. arboreum compared with own-rooted plants. These results indicate the potential benefits of grafting SHB onto V. arboreum rootstock, particularly under marginal soil conditions. However, a complete economic analysis that also takes into account any differences in longevity between the two systems must be done to determine whether the benefits of using grafting are feasible financially for the grower.
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43

Khymytsia, N. "Analysis of the Content of Bachelors’ Training in Communications at the Universities of the USA." Visnyk of Kharkiv State Academy of Culture, no. 64 (December 26, 2023): 61–75. http://dx.doi.org/10.31516/2410-5333.064.04.

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The relevance of the article. Today is characterized by great attention to the communicative component in all areas of society, because it is communication that plays an important role in the establishment of a new socio-cultural order and in the reproduction of its structures. Such challenges place rather high demands on both personal and professional qualities of future communication specialists. Therefore, the training of mobile, creative, competent, successful, competitive communication specialists in the conditions of socio-economic changes in Ukraine is the main task facing higher education. It is possible to ensure the proper quality of training of domestic communication specialists based on the creative use of pedagogical experience accumulated throughout the history of the development of education in Ukraine and foreign countries. Despite the large number of studies devoted to the actual issues of training specialists, the experience of innovative approaches to the provision of educational services in the USA has not yet been sufficiently studied. Meanwhile, the rapid application of innovation in the organization of the educational process, in particular in the field of training mobile, creative, competent, successful, competitive communication specialists, is a necessary condition for the development of Ukrainian society. The purpose of the article — study of the experience of the USA regarding the organization of professional training of bachelors in communications, research of the content and features of the curricula of leading American educational institutions that offer education at the educational level “Bachelor of Communications” with the aim of using such approaches in the practice of Ukrainian universities. The methodology. Analytical method, methods of description, comparison and generalization were applied for the research. The results. The issue of training communication specialists in the leading universities of the United States of America in the bachelor’s program was considered. Data on the popularity and demand of specialists who have completed a bachelor’s degree in communications in the USA are presented. A review of the content of bachelor’s training programs in communications at more than 20 US universities was carried out. The peculiarities of bachelor’s training in communications at the University of Miami (The University of Miami) and Florida International University (Florida International University, FIU) are analyzed. Emphasis is placed on the need to study, adapt and implement the positive experience of US universities regarding the training of communication specialists in Ukrainian universities. The scientific novelty. This study offers an analysis of the content of bachelor’s programs of leading American educational institutions that offer studies at the educational level “Bachelor of Communications” in 2023. The practical significance. Research materials and conclusions can be used to adapt and implement the experience of US universities in training communication specialists in Ukrainian universities, in particular, to improve the content of educational programs. Conclusions. Effective development of modern communications is impossible without improving the quality of training bachelors in communications in higher educational institutions of various types and forms of ownership in Ukraine. To solve this problem, it is necessary to take into account the experience accumulated throughout the history of the development of education in Ukraine and foreign countries. Currently, the USA is one of the most popular places to get a bachelor’s degree in communications. The US universities offer a wide selection of bachelor’s programs in communications, among which the most popular are: Bachelor of Arts in Communications; Bachelor of Arts in Organizational and Strategic Communication; Bachelor of Business and Communications; Bachelor of Digital Media and Communications; Bachelor of Science in Interpersonal Communication; Bachelor of Science in Corporate Communications. The curriculum emphasizes students’ understanding of communication in a variety of contexts (interpersonal, intercultural, organizational, political, and mass communications) and prepares future professionals to be effective and well-rounded communicators. Such specialized skills will allow students to choose from a wide range of career opportunities after completing the program in a variety of industries.
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Holcomb, G. E., and P. J. Cox. "First Report of Basil Leaf Spot Caused by Pseudomonas cichorii in Louisiana and Cultivar Screening Results." Plant Disease 82, no. 11 (November 1998): 1283. http://dx.doi.org/10.1094/pdis.1998.82.11.1283d.

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A leaf spot of basil, Ocimum basilicum L., was observed on container-grown and field plantings of cultivars Aussie Sweet and Sweet Basil. The disease was of minor importance under field conditions, but was of potential economic importance in seedling production. Gray to black, watersoaked, necrotic spots commonly developed at leaf margins. Large numbers of bacteria were released from cut lesions when viewed by light microscopy. Single colony bacterial isolates were established on nutrient dextrose agar (NDA) and yeast extract-dextrose-calcium carbonate agar (YDC). Pathogencity tests were performed by misting a water suspension containing 104 bacterial cells per ml on healthy basil plants. Plants were held for 24 h in a dew chamber at 26°C and then moved to a greenhouse for observation. Typical leaf spots developed on inoculated plants in 2 days, but not on healthy control plants, and the bacterium was reisolated. The bacterium was characterized as a gram-negative, motile rod, negative for potato rot test, positive in tobacco hypersensitivity test, and oxidase positive. Isolates were identified as Pseudomonas cichorii according to the Biolog Microplate system (similarities ranged from 0.937 to 0.995). Screening tests were conducted by inoculating 15 basil cultivars, six replicates each, and rating them for disease severity based on a scale of 1 to 5 in which 1 = no disease and 5 = dead plants. Cultivars most resistant to bacterial leaf spot (ratings in parentheses are averages of two tests and those followed by the same letter are not significantly different according to Tukey's Studentized Range Test, P = 0.05) were Green Bouquet (2.0 a), Piccolo (2.2 a), Mrs. Burn's Lemon (2.2 a), Genovese (2.4 a), and Dark Opal (2.5 ab). Moderately susceptible cultivars were Bush Green (2.8 abc), Sweet Basil (2.8 abc), Large Green (2.9 abcd), Lemon (3.1 abcd), and Mexican Spice (3.6 bcd). The most susceptible cultivars were Lettuce Leaved (3.8 cd), Thai (3.8 cd), Napoletano (4.0 de), Green Ruffles (5.0 e), and Purple Ruffles (5.0 e). Bacterial leaf spot of basil caused by P. cichorii was first reported in the U.S. from Florida (1). Other bacterial diseases reported on basil include leaf blight from Egypt caused by P. syringae (2) and leaf necrosis from California caused by P. viridiflava (3). This is the first report on the occurrence of basil bacterial leaf spot in Louisiana and the first reported information on cultivar susceptibility. References: (1) S. M. Burgess et al. Proc. Fla. State Hortic. Soc. 99:249, 1986. (2) S. A. M. El-Sadek et al. Assiut J. Agric. Sci. 22:2, 1991. (3) E. L. Little et al. Plant Dis. 78:831, 1994.
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Belayneh, Bruk E., John D. Lea-Cox, and Erik Lichtenberg. "Costs and Benefits of Implementing Sensor-controlled Irrigation in a Commercial Pot-in-Pot Container Nursery." HortTechnology 23, no. 6 (December 2013): 760–69. http://dx.doi.org/10.21273/horttech.23.6.760.

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Irrigation scheduling in ornamental plant production is complex due to the large number of species grown by individual growers and the need to consider plant, environment, and substrate conditions to make correct irrigation decisions on a daily or more frequent basis. The engineering team in our project has developed a smart wireless sensor node that is capable of integrating outputs from a range of soil moisture and environmental sensors to schedule irrigation events. In addition, an advanced monitoring and control software enables growers to manage irrigation based on set-point or model-based protocols, which are then independently executed by the nodes, enhancing or replacing human decision making. During 2012, we implemented a sensor-controlled vs. grower-controlled irrigation study at a pot-in-pot nursery in Tennessee. Sensor networks were installed in two separate production blocks of 3-year-old dogwood (Cornus florida ‘Cherokee Brave’) and 2-year-old red maple (Acer rubrum ‘Autumn Blaze’) trees grown in 15- and 30-gal containers, respectively. One row of trees in each block was irrigated based on the average reading of soil moisture sensors inserted in individual trees using micropulse irrigation, i.e., sensor controlled. Trees in the adjacent row and the rest of the block were independently irrigated by the grower using standard practices, i.e., grower controlled. Sensor volumetric water content (VWC) readings and irrigation volumes were logged by nodes on a 15-min basis and were relayed to a base station on the farm. For the study period between Mar. 2012 and Nov. 2012, average daily water applied by the grower-controlled irrigation to the dogwood block was 0.92 gal/tree, compared with 0.34 gal/tree applied using sensor-controlled irrigation; for red maple, these values were 1.72 gal/tree and 1.13 gal/tree, respectively. No significant differences in tree caliper or quality were noted between the two irrigation treatments in either species over the year. The cost of water for this particular operation was negligible consisting only of pumping costs, as water is drawn from a perennial stream with excellent water quality. Consequently, a conservative return on investment for a wireless sensor network capable of covering the entire operation was 37.5%, corresponding to a payback period of 2.7 years, associated almost entirely from a reduction in irrigation management time. Pricing in a nominal cost for water of $326 per acre-foot ($1 per 1000 gal) increased annualized net savings 9-fold, reducing the payback period to less than 4 months. This analysis did not factor in additional economic benefits such as reductions in production time, losses due to disease, or increased plant quality, which have been associated with the use of sensor-based irrigation control in other studies.
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Datta, Y. "How America Became an Economic Powerhouse on the Backs of African-American Slaves and Native Americans." Journal of Economics and Public Finance 7, no. 5 (December 1, 2021): p121. http://dx.doi.org/10.22158/jepf.v7n5p121.

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The objective of this paper is to make the case that the United States became an economic super-power in the nineteenth century on the backs of African-American slaves and Native Americans.It was in 1619, when Jamestown colonists bought 20-30 slaves from English pirates. The paper starts with ‘The 1619 Project’ whose objective is to place the consequences of slavery--and the contributions of black Americans--at the very center of the story we tell ourselves about who we are as a nation.Slavery was common in all thirteen colonies, and at-least twelve Presidents owned slaves. The enslaved people were not recognized as human beings, but as property: once a slave always a slave.The U.S. Constitution, adopted in 1788, never mentions slavery, yet slavery is at the very heart of the constitution. The U.S. government used the Declaration of Independence as a license to commit genocide on the Native Americans, and to seize their land.Racist ideas have persisted throughout American history, based on the myth that blacks are intellectually inferior compared to whites. However, in a 2012 article in the Scientific American, the authors reported that 85.5% of genetic variation is within the so-called races, not between them. So, the consensus among Western researchers today is that human races do not represent a scientific theory, but are sociocultural constructs.After end of the Civil War, the 13th Amendment to the U.S. Constitution abolished slavery in America, and the 15th Amendment protected the voting rights of African Americans.However, in the Confederate South, Jim Crow laws legalized racial segregation between 1870-1968. In 1965, thanks to the Civil Rights movement, the Voting Rights Act was passed to overcome barriers created by Jim Crow laws to the legal rights of African Americans under the 15th Amendment.British and American innovations in cotton technology sparked the Industrial Revolution during the latter part of the eighteenth century. The British cotton manufacturing exploded in the 1780s. Eighty years later in 1860, Manchester, England stood at the center of a world-spanning empire—the empire of cotton. There were three pillars of the Industrial Revolution. One was the centuries-earlier conquest by Europeans of a colossal expanse of lands in the New World. It was the control of huge territories in America, that made monoculture farming of cotton possible. Second was that the Europeans drastically—and unilaterally--altered the global competitive landscape of cotton. They did it by using their military might, and the willingness to use it—often violently--to their advantage.The third—and the most important--was slavery: without which there would be no Industrial Revolution. America was tremendously suited for cotton production. The climate and soil of a large part of American South met the conditions under which the cotton plant thrived. More importantly, the plantation owners in America commanded unlimited supplies of the three crucial ingredients that went into the production of cotton: labor, land, and credit. And this was topped by their unbelievable political power.In 1793 Eli Whitney’s revolutionary cotton gin increased ginning productivity fifty times, and thus removed the bottleneck of removing seeds from cotton. Because of relying on monoculture farming, the problem the cotton planters were facing was soil exhaustion. So, they wanted the U.S. government to acquire more land. Surprisingly, in 1803 America was able to strike an unbelievable deal with the French--the Louisiana Purchase--which doubled the territory of the United States. In 1819 America acquired Florida from Spain, and in 1845 annexed Texas from Mexico.Between 1803 and 1838, under President Andrew Jackson, America fought a multi-front war against the Native Americans in the Deep South, and expropriated vast tracts of their land, that culminated in the ethnic cleansing of the Deep South.With an unlimited supply of land—and slave labor--even soil exhaustion did not slow down the cotton barons; they just moved further west and farther south. New cotton fields now sprang up in the sediment-rich lands along the banks of Mississippi. So swift was this move westward that, by the end of the 1830s, Mississippi was producing more cotton than any other southern state. By 1860, there were more millionaires per capita in Mississippi Valley than anywhere else in America.The New Orleans slave market was the largest in America--where 100,000 men, women, and children were packaged, priced, and sold.The entry of the United States in the cotton market quickly began to reshape the global cotton market. By 1802 America was the single-most supplier of cotton to Britain.For eighty years--from the 1780s to 1865--almost a million people were herded down the road from the upper South to the lower South and the West, to toil on cotton plantations. The thirty-odd men walked in coffles, the double line hurrying in lock-step. Each hauled twenty pounds of iron, chains that draped from neck-to-neck, and wrist-to-wrist, binding them all together. They walked for miles, days, and weeks, and many covered over 700 miles.The plantation owners devised a cruel system of controlling their slaves that the enslaved called “the pushing system.” This system constantly increased the number of acres each slave was expected to cultivate. In 1805 each “hand” could tend to five acres of a cotton field. Fifty years later that target had been doubled to ten acres.Overseers closely monitored enslaved workers. Each slave was assigned a daily quota of number of pounds of cotton to pick. If the worker failed to meet it, he received as many lashes on his back as the deficit. However, if he overshot his quota, the master might “reward” him by raising his quota the next day.One of the most brutal weapons the planters used against the slaves, was the whip: ten feet of plaited cowhide. When facing the specter of an overseer’s whip, slaves were so terrified that they could not speak in sentences. They danced, trembled, babbled, and lost control of their bodies.When seeking a loan, the planters used slaves as a collateral. With extraordinarily high returns from their businesses, the planters began to expand their loan portfolio: sometimes using the same slave worker as collateral for multiple mortgages. The American South produced too much cotton. However, consumer demand could not keep up with the excessive supply, that then led to a precipitous fall in prices, which, in turn, set off the Panic of 1837. And that touched off a major depression.The slaveholders were using advanced management and accounting practices long before the techniques that are still in use today.The manufacture of sugar from sugarcane began in Louisiana Territory in 1795. In sugar mills, children, alongside with adults, toiled like factory workers with assembly-like precision and discipline under the constant threat of boiling hot kettles, open furnaces, and grinding rollers. To attain the highest efficiency, sugar factories worked day and night where there is no distinction as to the days of the week. Fatigue might mean losing an arm to the grinding rollers, or being flayed for not being able to keep up. Resistance was often met with sadistic cruelty.The expansion of slavery in the first eight decades after American independence, drove the evolution and modernization of the United States. In the course of a single life time, the South grew from a narrow coastal strip of worn-out tobacco plantations, to a continental cotton empire. As a result, the United States became a modern, industrial, and capitalistic economy. This is the period in which America rose from being a minor European trading partner, to becoming the world’s leading economy. Finally, we hope that we have successfully been able to make the argument that America became an economic powerhouse in the nineteenth century not only on the backs of African-American slaves, but also on the genocide of Native Americans, and their stolen lands.
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47

Freeman, S., G. Otero-Colina, G. Rodríguez-Alvarado, S. Fernández-Pavía, M. Maymon, R. C. Ploetz, T. Aoki, and K. O'Donnell. "First Report of Mango Malformation Disease Caused by Fusarium pseudocircinatum in Mexico." Plant Disease 98, no. 11 (November 2014): 1583. http://dx.doi.org/10.1094/pdis-04-14-0375-pdn.

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Mango (Mangifera indica L.) malformation disease (MMD) is one of the most important diseases affecting this crop worldwide, causing severe economic loss due to reduction of yield. After the first report in India in 1891 (3), MMD has spread worldwide to most mango-growing regions. Several species of Fusarium cause the disease, including F. mangiferae in India, Israel, the USA (Florida), Egypt, South Africa, Oman, and elsewhere; F. sterilihyphosum in South Africa and Brazil; F. proliferatum in China; F. mexicanum in Mexico; and recently, F. tupiense in Brazil (1,2,3,4). Besides F. mexicanum, F. pseudocircinatum, not yet reported as a causal agent of MMD, was isolated in Mexico from affected inflorescences and vegetative malformed tissues (4). Symptoms of vegetative malformation caused by F. pseudocircinatum included hypertrophied, tightly bunched young shoots, with swollen apical and lateral buds producing misshapen terminals with shortened internodes and dwarfed leaves. Infected inflorescences of primary or secondary axes on affected panicles were shortened, thickened, and highly branched, while the peduncles became thick, remained green and fleshy, and branches profusely resembled a cauliflower in shape and size (3). Ten isolates of F. pseudocircinatum were recovered from cultivars Ataulfo, Criollo, Haden, and Tommy Atkins in Guerrero, Campeche, and Chiapas states and characterized. Isolates produced mostly 0-septate but occasionally 1- to 3-septate oval, obovoid, or elliptical aerial conidia (0-septate: 4 to 19 [avg. 8.7] × 1.5 to 4 [avg. 2.6] μm) in false heads in the dark and in short false chains under black light, unbranched or sympodially branched prostrate aerial conidiophores producing mono- and polyphialides, and sporodochia with straight or falcate conidia that were mostly 3- to 5-septate, but sometimes up to 7-septate (3-septate: 25 to 58 [avg. 41] × 2 to 3.3 [avg. 2.9] μm; 5-septate: 33.5 to 76.5 [avg. 56.7] × 2.5 to 6 [avg. 3.5] μm). Circinate sterile hyphae were rarely formed. Two representative isolates, NRRL 53570 and 53573, were subjected to multilocus molecular phylogenetic analyses of portions of five genes: nuclear large subunit 28S ribosomal RNA, β-tubulin, calmodulin, histone H3, and translation elongation factor (TEF)-1α (GenBank GU737456, GU737457, GU737290, GU737291, GU737371, GU737372, GU737425, GU737426, GU737398, and GU737399). Two pathogenicity tests were conducted with NRRL 53570 and 53573 on healthy 2-year-old nucellar seedlings of polyembryonic Criollo; 20 μl conidial suspensions (5 × 106 conidia/ml) of each isolate and water controls were inoculated separately on 15 buds on 3 different trees, as described previously (1). The following conditions were used in experiment 1: 24 to 27°C with light intensity of 16.2 to 19.8 •Mol m−2s−1 in the range of 400 to 700 nm, and photoperiods of 14 h light and 10 h dark. Typical vegetative disease symptoms were discernible in plants inoculated with NRRL 53570 (20%) and 53573 (7%) after 8 months. In experiment 2, after 3 months growth under the above conditions, seedlings were transferred to an outdoor nursery in Iguala, Guerrero. Typical vegetative symptoms of MMD were observed in 86.7 and 13.3% of the buds inoculated with F. pseudocircinatum NRRL 53570 and 53573, respectively, after 9 months. Isolates from typical symptomatic vegetative buds were confirmed as F. pseudocircinatum by sequencing a portion of their TEF-1α gene, thus fulfilling Koch's postulates. This is the first report of F. pseudocircinatum as a causal agent of MMD. References: (1) S. Freeman et al. Phytopathology 89:456, 1999. (2) C. S. Lima et al. Mycologia 104:1408, 2012. (3) W. F. O. Marasas et al. Phytopathology 96:667, 2006. (4) G. Otero-Colina et al. Phytopathology 100:1176, 2010.
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48

Gamayunova, V. V., T. О. Kasatkina, and T. V. Baklanova. "Agroeconomic Assessment of Utilization Biologics in the Cultivation of Spring Barley in the Conditions of the Southern Steppe of Ukraine." Agrology 4, no. 1 (2021): 65–70. http://dx.doi.org/10.32819/021008.

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The results of research conducted in 2016‒2018 on the southern chernozem andthe effect of two varieties of spring barley extra-root feed on the formation of grain productivityare given. Based on the results obtained, indicators of agroeconomic efficiency and their changewere determined under the influence of this event (as one of the most important elements of technology)in growing the crop, including in terms of varieties. Despite the increase in productioncosts, the treatment of plant seeding with biologics contributed to a certain increase in notional netprofit, which increased with the number of feeds, reaching the highest values if used to optimizethe nutrition of Fresh florid (300 g/ha). One feeding of Stalker variety is determined at the levelof 5927, two feeding ‒ 6890, three ‒ 7320 UAH, and 3857 UAH/ha ‒ in control. The mentionedindicators were 5968, 6808, 7771 and 3980 UAH/ha, respectively, when the spring barley varietyVakula was grown. One of the most important indicators of economic efficiency decreased‒ the cost of growing a unit of production with an increase in the number of extracurricular feeding.The lowest indicator was the cost price of production of top – dressing with Fresh florid(300 g/ha ‒ according to the Stalker variety): with one extracorporeal sowing ‒ 2269.4; two treatments‒ 2173.5, and three ‒ 2166.7 UAH/t; in control, this indicator was 2638.5 UAH/t. Thesevalues were, respectively, 2262.3; 2185,8; 2105.4 and 2608.0 UAH/t when the barley spring varietyVakula was growing. The use of other biological preparations led to a certain increase in thecost of growing spring barley. The highest values were recorded when using Organic D-2 M withonly one feeding in the plant phase. In this version of the experiment the cost of growing a unitof production even slightly exceeded control, when Vakula variety was grown. The cultivation ofspring barley on the basis of resource saving using modern re-regulating substances and biologicalpreparations is advisable, because it increases not only the level of grain yield, but also themain indicators of agro-economic efficiency - net profit and profitability, and unit cost, on thecontrary, decreases. It was established that by optimizing the nutrition of spring barley on thebasis of resource saving by treating crops in the main growing seasons with biological products,along with increasing grain yield and changes in its quality, other indicators of agro-economicefficiency of crop production increase. The level of profitability in control (treatment of plantswith water) for the cultivation of barley of the spring variety Stalker was 59.2%, then in themost optimal food options it reached 93.8%, and for the Vakula variety they were determined61.0 and 99.5% respectively. The obtained agroeconomic indicators in the cultivation of springbarley made it possible to substantiate the following claims: the use of biologics to treat thesowing of spring barley plants during the main periods of vegetation increases the yield and costof grown grain. It was determined that the level of profitability of growing spring barley witha large number of feeds grew. The indicator reached its maximum value with three times theplanting of barley plants of the spring variety Vakula with Fresh florid, that is, 300 g/ha (99.5%).Two plant crops with this drug provided profitability at the level of 99.1%, and one ‒ 85.6, (incontrol ‒ 61.0%). The highest profitability of cultivation was ensured by the use of Fresh florid(300 g/ha) for optimizing nutrition three times for vegetation ‒in the phases of tillering, shootingand heading. The lowest level of profitability was ensured by the use of the preparation OrganicD-2 M for feeding barley of the spring variety Vakula: one treatment of plants ‒ at the level of60.6%, two ‒ 72.5, three treatments ‒ 78.2%. According to the results of research conductedwith two varieties of spring barley, the best biological product and terms of fertilization weredetermined in order to increase grain yield and improve its quality and agro-economic efficiencybased on the calculation of cost economic indicators. The expediency of nutrition optimizationby using modern biological products for spring barley cultivation has been confirmed.
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49

Ahearn, Mary, and James Sterns. "Direct-to-Consumer Sales of Farm Products: Producers and Supply Chains in the Southeast." Journal of Agricultural and Applied Economics 45, no. 3 (August 2013): 497–508. http://dx.doi.org/10.1017/s1074070800005010.

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Given the geography and agroclimatic conditions of the Southeast, coupled with continued population expansion from in-migration, local foods markets may be a promising niche market for some farms in the region. The Southeast has more small farms than any other U.S. region. Using farm-level data, we address the question of how successful southeastern farms engaged in direct sales to consumers differ from other farms. We also include a case study of a marketing association in the panhandle of Florida. In both analyses, we focus on the role of the supply chain for direct sales in explaining farm returns.
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50

Jeon, Y. H., and W. Cheon. "First Report of Leaf Blight of Japanese Yew Caused by Pestalotiopsis microspora in Korea." Plant Disease 98, no. 5 (May 2014): 691. http://dx.doi.org/10.1094/pdis-08-13-0821-pdn.

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Worldwide, Japanese yew (Taxus cuspidata Sieb. & Zucc.) is a popular garden tree, with large trees also being used for timber. In July 2012, leaf blight was observed on 10% of Japanese yew seedling leaves planted in a 500-m2 field in Andong, Gyeongsangbuk-do Province, South Korea. Typical symptoms included small, brown lesions that were first visible on the leaf margin, which enlarged and coalesced into the leaf becoming brown and blighted. To isolate potential pathogens from infected leaves, small sections of leaf tissue (5 to 10 mm2) were excised from lesion margins. Eight fungi were isolated from eight symptomatic trees, respectively. These fungi were hyphal tipped twice and transferred to potato dextrose agar (PDA) plates for incubation at 25°C. After 7 days, the fungi produced circular mats of white aerial mycelia. After 12 days, black acervuli containing slimy spore masses formed over the mycelial mats. Two representative isolates were further characterized. Their conidia were straight or slightly curved, fusiform to clavate, five-celled with constrictions at the septa, and 17.4 to 28.5 × 5.8 to 7.1 μm. Two to four 19.8- to 30.7-μm-long hyaline filamentous appendages (mostly three appendages) were attached to each apical cell, whereas one 3.7- to 7.1-μm-long hyaline appendage was attached to each basal cell, matching the description for Pestalotiopsis microspora (2). The pathogenicity of the two isolates was tested using 2-year-old plants (T. cuspidata var. nana Rehder; three plants per isolate) in 30-cm-diameter pots filled with soil under greenhouse conditions. The plants were inoculated by spraying the leaves with an atomizer with a conidial suspension (105 conidia/ml; ~50 ml on each plant) cultured for 10 days on PDA. As a control, three plants were inoculated with sterilized water. The plants were covered with plastic bags for 72 h to maintain high relative humidity (24 to 28°C). At 20 days after inoculation, small dark lesions enlarged into brown blight similar to that observed on naturally infected leaves. P. microspora was isolated from all inoculated plants, but not the controls. The fungus was confirmed by molecular analysis of the 5.8S subunit and flanking internal transcribed spaces (ITS1 and ITS2) of rDNA amplified from DNA extracted from single-spore cultures, and amplified with the ITS1/ITS4 primers and sequenced as previously described (4). Sequences were compared with other DNA sequences in GenBank using a BLASTN search. The P. microspora isolates were 99% homologous to other P. microspora (DQ456865, EU279435, FJ459951, and FJ459950). The morphological characteristics, pathogenicity, and molecular data assimilated in this study corresponded with the fungus P. microspora (2). This fungus has been previously reported as the causal agent of scab disease of Psidium guajava in Hawaii, the decline of Torreya taxifolia in Florida, and the leaf blight of Reineckea carnea in China (1,3). Therefore, this study presents the first report of P. microspora as a pathogen on T. cuspidata in Korea. The degree of pathogenicity of P. microspora to the Korean garden evergreen T. cuspidata requires quantification to determine its potential economic damage and to establish effective management practices. References: (1) D. F. Farr and A. Y. Rossman, Fungal Databases, Syst. Mycol. Microbiol. Lab. Retrieved from http://nt.ars-grin.gov/fungaldatabases/ (2) L. M. Keith et al. Plant Dis. 90:16, 2006. (3) S. S. N. Maharachchikumbura. Fungal Diversity 50:167, 2011. (4) T. J. White et al. PCR Protocols. Academic Press, San Diego, CA, 1990.
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