Academic literature on the topic 'Flow cell'

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Journal articles on the topic "Flow cell"

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Hess, G. P., R. W. Lewis, and Y. Chen. "Cell-Flow Technique." Cold Spring Harbor Protocols 2014, no. 10 (2014): pdb.prot084160. http://dx.doi.org/10.1101/pdb.prot084160.

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Ahmed, Afzal, Mir Shabbar Ali, and Toor Ansari. "Modelling Heterogeneous and Undisciplined Traffic Flow using Cell Transmission Model." International Journal of Traffic and Transportation Management 02, no. 01 (2020): 01–05. http://dx.doi.org/10.5383/jttm.02.01.001.

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This research calibrates Cell Transmission Model (CTM) for heterogeneous and non-lane disciplined traffic, as observed in Pakistan and some other developing countries by constructing a flow-density fundamental traffic flow diagram. Currently, most of the traffic simulation packages used for such heterogonous and non-lane-disciplined traffic are not calibrated for local traffic conditions and most of the traffic flow models are developed for comparatively less heterogeneous and lane-disciplined traffic. The flow-density fundamental traffic flow diagram is developed based on extensive field data collected from Karachi, Pakistan. The calibrated CTM model is validated by using actual data from another road and it was concluded that CTM is capable of modelling heterogeneous and non-lane disciplined traffic and performed very reasonably. The calibrated CTM will be a useful input for the application of traffic simulation and optimization packages such as TRANSYT, SIGMIX, DISCO, and CTMSIM.
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Maheskumar, Pon, S. A. Srinivasan, M. Arjunraj, and B. Sakthivel. "Numerical Study on Performance of Single Flow Channel PEM Fuel Cell for Different Flow Channel Configurations." Journal of Advanced Research in Dynamical and Control Systems 11, no. 11 (2019): 444–52. http://dx.doi.org/10.5373/jardcs/v11i11/20193349.

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KOZAKAI, Masaya, Tsutomu OKUSAWA, Hiroyuki SATAKE, and Ko TAKAHASHI. "C211 INVESTIGATION OF POROUS GAS FLOW FIELD IN POLYMER ELECTROLYTE MEMBRANE FUEL CELL(Fuel Cell-2)." Proceedings of the International Conference on Power Engineering (ICOPE) 2009.2 (2009): _2–237_—_2–242_. http://dx.doi.org/10.1299/jsmeicope.2009.2._2-237_.

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Degawa, Tomohiro, and Tomomi Uchiyama. "NUMERICAL SIMULATION OF THE BUBBLY FLOW AROUND A RECTANGULAR CYLINDER BY VORTEX IN CELL METHOD(Multiphase Flow)." Proceedings of the International Conference on Jets, Wakes and Separated Flows (ICJWSF) 2005 (2005): 235–40. http://dx.doi.org/10.1299/jsmeicjwsf.2005.235.

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Melchior, Benoît, and John A. Frangos. "Shear-induced endothelial cell-cell junction inclination." American Journal of Physiology-Cell Physiology 299, no. 3 (2010): C621—C629. http://dx.doi.org/10.1152/ajpcell.00156.2010.

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Atheroprone regions of the arterial circulation are characterized by time-varying, reversing, and oscillatory wall shear stress. Several in vivo and in vitro studies have demonstrated that flow reversal (retrograde flow) is atherogenic and proinflammatory. The molecular and structural basis for the sensitivity of the endothelium to flow direction, however, has yet to be determined. It has been hypothesized that the ability to sense flow direction is dependent on the direction of inclination of the interendothelial junction. Immunostaining of the mouse aorta revealed an inclination of the cell-cell junction by 13° in direction of flow in the descending aorta where flow is unidirectional. In contrast, polygonal cells of the inner curvature where flow is disturbed did not have any preferential inclination. Using a membrane specific dye, the angle of inclination of the junction was dynamically monitored using live cell confocal microscopy in confluent human endothelial cell monolayers. Upon application of shear the junctions began inclining within minutes to a final angle of 10° in direction of flow. Retrograde flow led to a reversal of junctional inclination. Flow-induced junctional inclination was shown to be independent of the cytoskeleton or glycocalyx. Additionally, within seconds, retrograde flow led to significantly higher intracellular calcium responses than orthograde flow. Together, these results show for the first time that the endothelial intercellular junction inclination is dynamically responsive to flow direction and confers the ability to endothelial cells to rapidly sense and adapt to flow direction.
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Faizar Abdurrahman, Faizar Abdurrahman, Norhana Arsad Norhana Arsad, Sabiran Sabiran, and Harry Ramza Harry Ramza. "Simple design flow injection PMMA acrylic sample cell for nitrite determination." Chinese Optics Letters 12, no. 4 (2014): 043002–43004. http://dx.doi.org/10.3788/col201412.043002.

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Ley, Klaus. "Cell Adhesion under Flow." Microcirculation 16, no. 1 (2009): 1–2. http://dx.doi.org/10.1080/10739680802644415.

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Shi, Zheng, Zachary T. Graber, Tobias Baumgart, Howard A. Stone, and Adam E. Cohen. "Cell Membranes Resist Flow." Cell 175, no. 7 (2018): 1769–79. http://dx.doi.org/10.1016/j.cell.2018.09.054.

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Segal, S. S. "Cell-to-cell communication coordinates blood flow control." Hypertension 23, no. 6_pt_2 (1994): 1113–20. http://dx.doi.org/10.1161/01.hyp.23.6.1113.

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Dissertations / Theses on the topic "Flow cell"

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Rabodzey, Aleksandr. "Flow-induced mechanotransduction in cell-cell junctions of endothelial cells." Thesis, Massachusetts Institute of Technology, 2006. http://hdl.handle.net/1721.1/41586.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Biological Engineering Division, 2006.<br>Includes bibliographical references (leaves 86-92).<br>Endothelial cells show an unexpected behavior shortly after the onset of laminar flow: their crawling speed decreases ~40% within the first 30 min, but only in a confluent monolayer of endothelial cells, not in subconfluent cultures, where cell-cell interactions are limited. This led us to study early shear effects on cell-cell adherens junctions. We found a 30±6% increase in the number of VE-cadherin molecules in the junctions. The strength of interactions of endothelial cells with surfaces coated with recombinant VE-cadherin protein also increased after laminar flow. These observations suggest that endothelial cell junction proteins respond to flow onset. The process of clustering may induce diffusion of monomers to the junction area, resulting in an overall increase in VE-cadherins in the junctions. To directly confirm the role of adherens junctions in the decrease in cell crawling speed, we used siRNA-knockdown technique to produce cells lacking VE-cadherin. These cells showed no decline in crawling speed under flow. Our interpretation is consistent with previous data on junction disassembly 8 hr after flow onset. The speed of endothelial cell crawling returns to the original level by that time, and junctional disassembly may explain that phenomenon. In order to understand better the change in VE-cadherin distribution under flow and during junction formation and remodelling, we developed a mathematical model of VE-cadherin redistribution in endothelial cells. This model allowed us to develop a quantitative framework for analysis of VE-cadherin redistribution and estimate the amount of protein in the junctions and on the apical surface. In addition to that, the model explains rapid junction disassembly in the leukocyte transmigration and junction formation in subconfluent cells.<br>(cont.) These studies show that intercellular adhesion molecules are important in the force transmission and shear stress response. Their role, however, is not limited to flow mechanotransduction. Intercellular force transmission has an important application - organ development and, specifically, angiogenesis. We studied the role of VE-cadherin in vessel development in HUVECs and showed that VE-cadherin-null cells do not form vessels in the in vitro assay. This observation confirms the important role of intercellular force transmission in response to external force caused by flow or exerted by other cells.<br>by Aleksandr Rabodzey.<br>Ph.D.
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LASAGNA, DAVIDE. "Flow physics and control of trapped vortex cell flows." Doctoral thesis, Politecnico di Torino, 2013. http://hdl.handle.net/11583/2518621.

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The main objective of this work is to investigate on the physics and on the control of the flow in a Trapped Vortex Cell, often referred to as TVC in the following. A TVC is a cavity with a particular geometry, which is optimised to trap a vortical structure. This configuration has recently gained interest has a device to control the flow past thick airfoils, but fundamental research is still required to make this technique effective. Specifically, a first goal of this work is to investigate on the fundamental physics of this flow, by studying the basic elements and the dominant phenomena. In fact, this flow field is the result of the complex interaction of several flows, such as the upstream boundary layer, the shear layer detaching from the cavity leading edge, the vortex core, and the boundary layer developing downstream. A further issue of interest addressed in this work is that related to the role of unsteadiness of the cell flow, and in particular of the shear layer, whose self-sustained oscillations are a common feature of open-cavity flows. The understanding of the driving physical mechanisms of the base flow is required to successfully proceed in developing a control strategy aiming at the control of the flow, because it is necessary to manipulate this flow in order to make the TVC an effective control device. Therefore, a second goal is to study and compare two different control techniques targeting the cavity flow. The first of the two is steady suction of the flow in the cell and has been already applied in past researches, but some additional insight into its effects on the base flow is required. The second proposed control technique is open-loop control with a synthetic jet actuator, a more efficient device whose unsteady action can couple to or drive the relevant mechanisms of the flow. Furthermore, open-loop control studies with a synthetic jet are propaedeutic for closed-loop control of the flow, briefly investigated in the last part of research.
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Kucukal, Erdem. "BIOMIMETIC MICROFLUIDIC PLATFORMS FOR MONITORING CELLULAR INTERACTIONS IN MICROSCALE FLOW." Case Western Reserve University School of Graduate Studies / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=case1576231265150031.

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Ofsthun, Norma Jean. "Cross-flow membrane filtration of cell suspensions." Thesis, Massachusetts Institute of Technology, 1989. http://hdl.handle.net/1721.1/14481.

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Han, Tian. "Flow cell separation in fluctuating g-field." Thesis, Brunel University, 2015. http://bura.brunel.ac.uk/handle/2438/11105.

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Field flow fractionation of particles in rotating coiled column has been investigated in recent year. In contrast to the classical mode of field flow fractionation in narrow channels, the use of rotating coiled columns offers the possibility of large sample loading. In this thesis, the potential for new cell separation methods based on the use of flow fractionation in fluctuating g-fields generated in rotating coil columns is examined. The effects of operational conditions (flow rate and rotational speed – Chapter 3 and Chapter 5); cell properties (cell flexibility – Chapter 4); and column shapes (different inner diameters and coil geometries – Chapter 6) on the flow behaviour of a model system of red blood cells (RBCs) from different species, which differ markedly in size, shape & density, flowing in a single phase of buffered saline have been characterised. Operational Conditions: For a particular rotational speed, there was a minimum flow rate which caused all the cells to be retained in the column and a maximum flow rate at which all cells were eluted. Both the minimum and maximum flow rate were increased when a higher rotational speed was applied. Differences in the behaviour of sheep & hen RBCs have been used to develop a separation method using a continuously increasing flow gradient. This separation could be speeded up by using a step flow gradient. The effects of cell load and rotational direction on the behaviour of RBCs in the column was also studied in this thesis. Cell Properties: The minimum flow rate was found to correlate with cell diameter/cell volume of the RBCs as expected for a sedimentation related process and was partially described by a theoretic equation developed for particles by Fedotov and colleagues (Fedotov et al. 2005). However cell dependent departures from this equation were found which appear to indicate that cell specific surface properties may also be involved for cells (Chapter 3). By contrast the maximum flow rate showed no correlation with cell diameter/cell volume. An effect of cell deformability on the flow separation behaviour of the cells has been demonstrated. Chemical fixation of sheep RBCs with glutaraldehyde rendered the normally deformable RBCs rigid and non-deformable and resulted in the fixed sheep RBCs eluting significantly earlier than unfixed sheep RBCs. This difference was great enough that a mixture of deformable (unfixed) and non-deformable (fixed) sheep RBCs could be separated. Fixed cells tended to show cell aggregation, which could be reduced by the addition of surfactant. Column Geometry: An effect of column shapes on the flow separation behaviour of cells has been demonstrated showing that the optimisation of column design is an important feature of this mode of cell separation. For columns with the same cross sectional area, a “horizontal” rectangular column provided better separation than a circular column and a “vertical” rectangular column gave the least efficient separation. A possible explanation for this behaviour is suggested the thinner sedimentation layer and less secondary flow. Differences in the behaviour of various species of RBCs in the “horizontal” rectangular column have been used to study the efficiency of separation of a mixture of sheep and hen RBCs, and a mixture of rabbit and hen RBCs. This work shows similarities and differences with other reports on cell/particle separations in rotating coiled columns in single phases and also in aqueous two phases systems (ATPS) and these are discussed. Fedotov P.S., Kronrod V.A. & Kasatonova O.N. (2005). Simulation of the motion of solid particles in the carries liquid flow in a rotating coiled column. J. Anal. Chem., 60, 4, 310-316.
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Choe, Juno. "Genomic analysis by single cell flow sorting /." Thesis, Connect to this title online; UW restricted, 2003. http://hdl.handle.net/1773/10850.

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Dive, C. "Flow cytoenzymology with special reference to cancer chemotherapy." Thesis, Open University, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384585.

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Camplejohn, Richard Stephen. "Cell kinetics and cancer." Thesis, University of Newcastle Upon Tyne, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.327272.

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Korn, Christian. "Stochastic dynamics of cell adhesion in hydrodynamic flow." Phd thesis, Universität Potsdam, 2007. http://opus.kobv.de/ubp/volltexte/2007/1299/.

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Allen, R. J. "Modelling the endothelial cell response to fluid flow." Thesis, University College London (University of London), 2009. http://discovery.ucl.ac.uk/16119/.

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In vitro endothelial cells respond to fluid flow by elongating in the direction of flow. How the mechanical signal is transformed into an organised and directed response is poorly understood. The most studied and crucial aspects to this response are; actin filament alignment, mechano-transduction, signal transduction, Rho GTPase localised activation and lamellipodium formation. The goal of this project is to understand how these separate facets interact and lead to a coordinated response. The flow is modelled over a 3D virtual cell, which naturally gives the force the flow exerts on the cell surface via a boundary integral representation. This force is coupled to a Kelvin-body model of mechano-transduction which links, via a focal adhesion associated protein, Src, to a partial differential equation model (PDE) of the Rho GTPases Rac and Rho. The PDEs are integrated over a 2D projection of the 3D cell giving a time course for protein concentration at any point in the cell. It is demonstrated that a mechano-transducer that can respond to the normal component of the force is likely to be a necessary (though perhaps not sufficient) component of the signalling network. In some processes cross talk between the GTPases is thought to be important in forming spatially segregated zones of activation, for example in the front and back of migratory cells. This research shows that local signalling in endothelial cells could be initiated by the force normal to the surface of the cell and maintained by limited diffusion. Modelling indicates the EC signalling response to fluid flow may be attenuated by a change in morphology. Rac and Rho activation and deactivation are validated against experimentally reported time courses that have been taken for whole cell averages. However it will be demonstrated that these time courses do not characterise the process and therefore there is a need for more quantitative local measure of protein activation.
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Books on the topic "Flow cell"

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Radbruch, Andreas, ed. Flow Cytometry and Cell Sorting. Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-662-02785-1.

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Radbruch, Andreas, ed. Flow Cytometry and Cell Sorting. Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-662-04129-1.

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A, Radbruch, ed. Flow cytometry and cell sorting. Springer-Verlag, 1992.

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R, Melamed Myron, Lindmo Tore, and Mendelsohn M. L, eds. Flow cytometry and sorting. 2nd ed. Wiley-Liss, 1991.

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1922-, Melamed Myron R., Lindmo T, and Mendelsohn Mortimer L, eds. Flow cytometry and sorting. 2nd ed. Wiley-Liss, 1990.

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G, Macey Marion, ed. Flow cytometry: Clinical applications. Blackwell Scientific Publications, 1994.

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E, Hart J., and George C. Marshall Space Flight Center., eds. The geophysical fluid flow cell experiment. National Aeronautics and Space Administration, Marshall Space Flight Center, 1999.

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1948-, Al-Rubeai Mohamed, and Emery A. Nicholas 1942-, eds. Flow cytometry applications in cell culture. Marcel Dekker, 1996.

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Gary, Durack, and Robinson J. Paul, eds. Emerging tools for single-cell analysis: Advances in optical measurement technologies. Wiley-Liss, 2000.

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W, Gray Joe, ed. Flow cytogenetics. Academic, 1989.

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Book chapters on the topic "Flow cell"

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Ortolani, Claudio. "Cell Sorting." In Flow Cytometry Today. Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-10836-5_21.

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Capo, Christian, Zohair Mishal, and Pierre Bongrand. "Use of Flow Cytometry to Analyze Cell-Cell Molecular Transfer." In Flow Cytometry. CRC Press, 2024. https://doi.org/10.1201/9781003574323-10.

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Crissman, Harry A., and Anthony J. Nastasi. "Cell Cycle and Cell Proliferation Markers." In Flow and Image Cytometry. Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-61115-5_7.

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Amblard, F. "Fluid Mechanical Properties of Flow Cytometers and Assessment Cell-Cell Adhesion Forces." In Flow Cytometry. Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-84616-8_13.

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Andreoni, C. "Immunomagnetic Particles for Cell Isolation." In Flow Cytometry. Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-84616-8_29.

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Bansal, Seema, Rishabh Chaudhary, Nitin Bansal, and Bikash Medhi. "Stem Cell and Microvesicles Analysis." In Flow Cytometry. Springer Nature Singapore, 2024. https://doi.org/10.1007/978-981-97-4553-1_22.

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Pollack, Alan, and Gaetano Ciancio. "Multiparameter Cell Cycle Analysis of G2-Arrest and Cell Death Following Ionizing Irradiation." In Flow Cytometry. CRC Press, 2024. https://doi.org/10.1201/9781003574323-3.

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Jacobberger, James W., R. Michael Sramkoski, and Tammy Stefan. "Multiparameter Cell Cycle Analysis." In Flow Cytometry Protocols. Humana Press, 2010. http://dx.doi.org/10.1007/978-1-61737-950-5_11.

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Cheetham, Mark, Derek Davies, Christopher Hall, Charlotte Christie Petersen, Reiner Schulte, and Rachael Walker. "Practicalities of Cell Sorting." In Flow Cytometry Protocols. Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3738-8_7.

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Jacobberger, James W., R. Michael Sramkoski, Tammy Stefan, and Philip G. Woost. "Multiparameter Cell Cycle Analysis." In Flow Cytometry Protocols. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7346-0_11.

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Conference papers on the topic "Flow cell"

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Marquardt, N., T. Hengsbach, M. Mauritz, B. Wirth, and K. P. Schäfers. "Cell Flow PET Simulations for Validation of Cell Tracking Algorithms." In 2024 IEEE Nuclear Science Symposium (NSS), Medical Imaging Conference (MIC) and Room Temperature Semiconductor Detector Conference (RTSD). IEEE, 2024. http://dx.doi.org/10.1109/nss/mic/rtsd57108.2024.10657932.

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Yun, Seok-Hyun Andy. "Multipass flow cytometry using laser cell barcoding." In High-Throughput Biophotonics: Imaging, Spectroscopy, and Beyond X, edited by Keisuke Goda and Kevin K. Tsia. SPIE, 2025. https://doi.org/10.1117/12.3040414.

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Miyasaka, M., K. Kishimoto, and S. Aoki. "A Study on Differential-Flow-Rate-Cell Corrosion in Seawater." In CORROSION 1995. NACE International, 1995. https://doi.org/10.5006/c1995-95287.

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Abstract Mechanisms of differential-flow-rate-cell corrosion (differential-aeration-cell corrosion caused by differential flow rates) of cast iron in seawater were studied. Potential and current density distributions produced by the differential-flow-rate-cell were measured on actual pumps and a model test cell. Boundary element analysis was also performed on differential-flow-rate-cell corrosion occurred in the model test cell. These studies demonstrate that differential-flow-rate-cell corrosion has characteristics similar to those of galvanic corrosion, and thus can be treated in the same manner as galvanic corrosion.
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Samimi, Kayvan, Ojaswi Pasachhe, Wenxuan Zhao, et al. "Autofluorescence lifetime flow cytometry and single cell deposition." In Multiscale Imaging and Spectroscopy VI, edited by Alex J. Walsh, Darren M. Roblyer, and Paul J. Campagnola. SPIE, 2025. https://doi.org/10.1117/12.3041767.

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Hensel, J. Peter, Randall S. Gemmen, Brian J. Hetzer, et al. "Fuel Cell Performance Improvements Using Cell-to-Cell Flow Distribution Control." In ASME 2004 2nd International Conference on Fuel Cell Science, Engineering and Technology. ASMEDC, 2004. http://dx.doi.org/10.1115/fuelcell2004-2482.

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Balanced flow distribution to each cell in a fuel cell stack plays a significant role in the stack being able to operate at maximum capability and efficiency. This paper discusses the performance improvements in proton exchange membrane fuel cell stacks that can be obtained by using cell-to-cell flow distribution control. In a specially instrumented four-cell stack that employs needle valves to externally control the air and fuel flows to each cell, fuel to a single cell was reduced. The V-I curves collected under these conditions (unbalanced) are compared to curves collected when the fuel flow to each cell was equal (balanced). Reducing the fuel flow to a single cell by 30% decreased the V-I curve cutoff load by 8.5% — demonstrating the negative effect that unbalanced fuel flows can have on stack performance. Typical fuel cell stacks have no dynamic means to keep flows in the stack balanced between the cells, but this work indicates that flow balancing among cells can extend the V-I curve for a fuel cell to higher current values, allowing fuel cell stacks to operate reliably at higher loading and fuel utilizations. Plans to use novel, custom-built micro-valves to dynamically balance flow to individual cells in a fuel cell stack are being pursued as a result of this work, and the status of this development effort is provided.
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KHORRAMI, M., and C. GROSCH. "Temporal stability of multiple-cell vortices." In 2nd Shear Flow Conference. American Institute of Aeronautics and Astronautics, 1989. http://dx.doi.org/10.2514/6.1989-987.

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Popova, M., P. Vorobieff, and M. Ingber. "Analysis of two- and three-particle motion in a Couette cell." In MULTIPHASE FLOW 2007. WIT Press, 2007. http://dx.doi.org/10.2495/mpf070301.

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Boronin, S., A. Osiptsov, and J. Desroches. "Flows of particle-laden Bingham fluids in a Hele-Shaw cell." In MULTIPHASE FLOW 2013. WIT Press, 2013. http://dx.doi.org/10.2495/mpf130121.

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Grega, Lisa M., and Steven Voinier. "Effect of Inlet Flow Conditions on Flow Uniformity in a PEM Fuel Cell." In ASME 2011 9th International Conference on Fuel Cell Science, Engineering and Technology collocated with ASME 2011 5th International Conference on Energy Sustainability. ASMEDC, 2011. http://dx.doi.org/10.1115/fuelcell2011-54233.

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The use of fuel cells as an alternative to traditional small scale power producing devices such as internal combustion engines or disposable batteries has continued to gain widespread acceptance. Flow maldistribution within cells in a stack continues to be an issue in fuel cell design and can adversely affect performance and longevity. Current research in this field has focused on effects of inlet configurations (plug flow versus circular inlet, for example) on the flow in a rectangular manifold and the resulting distribution into individual cells in the stack. In a typical small scale application, the piping which transports the reactant will contain bends in it. As these bends can introduce Dean vortices and flow asymmetries within the pipe flow, such conditions should be examined to determine whether they will affect the manifold flow and further impact cell maldistribution. A simplified scaled up model of a PEM fuel cell was fitted with different inlet flow configurations, including straight piping and piping containing a 90 degree bend prior to entering the manifold. Particle Image Velocimetry (PIV) was used to obtain mean and fluctuating velocity statistics within the manifold and in individual cells. These distributions will be compared with previous results obtained from this apparatus corresponding to a partially developed square inlet profile, as well as available experimental and computational data in the literature.
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Choban, Eric R., Piotr Waszczuk, Larry J. Markoski, Andrzej Wieckowski, and Paul J. A. Kenis. "Membraneless Fuel Cell Based on Laminar Flow." In ASME 2003 1st International Conference on Fuel Cell Science, Engineering and Technology. ASMEDC, 2003. http://dx.doi.org/10.1115/fuelcell2003-1728.

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An increasing societal demand for a wide range of small, often portable devices that can operate for an extended period of time without recharging has resulted in a surge of research in micropower sources. Most efforts in this area focus on downscaling of existing fuel cell technology such as the well-known proton exchange membrane (PEM) fuel cells. Here we study a novel concept for fuel cells: the use of laminar flow instead of a physical barrier such as a PEM to separate the fuel and oxidant streams. Laminar flow, i.e. low Reynolds number flow, is a property of fluid flow at the microscale: one or more liquid streams that are brought together under low Reynolds number conditions flow in parallel and contact with each other without turbulent mixing. Mass transport transverse to the direction of flow takes place by diffusion only. In our laminar flow-based fuel cell a fuel-containing stream and an oxidant-containing stream are brought together in laminar flow conditions with the electrodes placed on opposite walls within the channel. In un-optimized fuel cell configurations, current densities as high as 10 mA/cm2 are obtained at room temperature using different fuels such as methanol or formic acid vs. oxygen saturated solvents or other oxidants.
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Reports on the topic "Flow cell"

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Singh, Anjali. Ultimate Guide to Automated Cell Counter: Plus Purchasing Tips. ConductScience, 2022. http://dx.doi.org/10.55157/cs20220614.

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An automated cell counter is a machine that uses either image analysis or electrical impedance principles to count cells automatically. The electrical impedance principle involves measuring changes in electrical resistance as cells pass through an aperture, while the light-scattering principle observes how cells scatter light when exposed to it. There are four main types of automated cell counting methods: Coulter Counter, Image Analysis Method, Flow Cytometry, and Stereological Cell Counting. Each method has its benefits and limitations, offering faster and more objective cell counting compared to manual methods, but also facing challenges like cost and potential counting inaccuracies. To use an automated cell counter, samples are prepared by pipetting cell suspension onto counting slide chambers, and the machine then provides a total cell count per ml.
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CORSCADDENorscadden, Louise, and Arpaporn Sutipatanasomboon. The Definite Guide to Flow Cytometry for Scientists. ConductScience, 2022. http://dx.doi.org/10.55157/cs20221213.

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Flow cytometry is an analytical technique that examines cells suspended in fluids. It uses a built-in laser beam to illuminate individual cells as the fluid passes through. The illumination causes fluorescence and scattered lights, which are emitted and reflected from the examining cell. These lights are split and filtered onto detectors and converted into electrical signals.
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Wieder, Robert. Microfluidic Flow Retardation for Tagless Cancer Cell Analysis. Defense Technical Information Center, 2012. http://dx.doi.org/10.21236/ada566937.

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Wieder, Robert. Microfluidic Flow Retardation for Tagless Cancer Cell Analysis. Defense Technical Information Center, 2011. http://dx.doi.org/10.21236/ada549637.

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Heil, Cynthia A., Gabriel A. Vargo, David P. Fries, Ziaoling Ding, and David F. Millie. Flow Cytometer Based Biosensor for In-Field Cell Analysis. Defense Technical Information Center, 2003. http://dx.doi.org/10.21236/ada630296.

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Yompakdee, Chulee, and Sittiruk Roytrakul. Molecular target of an anti-cancer compound from leaves of Clausena harmandiana (Pierre). Chulalongkorn University, 2016. https://doi.org/10.58837/chula.res.2016.32.

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Clausena harmandiana (Pierre) Guillaumin or Song faa dong (in Thai), is classified in Family Rutaceae. Previous study, a coumarin compound designated CHA-01 was isolated from leave extract of C. harmandiana with inhibitory activity against calcium signaling in a ZDS1 null mutant yeast Saccharomyces cerevisiae (delta zds1). However, not much has been known on biological activity of this coumarin. In the past, some other coumarins were reported to contain anti-cancer activity. The aim of this research was to study molecular mechanism on antiproliferation activity of CHA-01 in Jurkat T cells. The results revealed that CHA-01 showed anti-proliferative activity in several cell lines including Jurkat (Lymphocytic leukemic cell line), KATO III (Stomach cancer cell line) and THP1 (Monocytic leukemic cell line) by MTT assay. Jurkat T cell line was the most sensitity cell line to CHA-01 treatment with IC50 value of 0.67 μM. It contained no cytotoxic activity against normal lymphocytes up to 10 μM. Flow cytometric analysis revealed that the CHA-01 caused cell cycle arrest at S phase in Jurkat T cells as a result from inhibition of DNA synthesis. Moreover, CHA-01 induced apoptotic cell death in the Jurkat T cells. Our results revealed the role(s) of CHA-01 on its anti-cancer activity especially against lymphocytic leukemia. Future study will utilize proteomic analysis on expression level of proteins in CHA-01 treated Jurkat T cells.
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Hosseini, Neda. Stereolithographic Fabrication of a Flow Cell For Improved Neurochemical Sensor Testing. Office of Scientific and Technical Information (OSTI), 2018. http://dx.doi.org/10.2172/1481062.

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Glasscott, Matthew, and Jason Ray. Accelerated corrosion of infrastructural seven-strand cables via additively manufactured corrosion flow cells. Engineer Research and Development Center (U.S.), 2023. http://dx.doi.org/10.21079/11681/47606.

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The purpose of this project was to generate an accelerated corrosion methodology capable of producing seven-strand cables with simulated corrosive defects for calibration of nondestructive analysis (NDA) techniques. An additively manufactured accelerated corrosion cell was motivated and designed. Previous attempts at accelerated electrochemical corrosion used a large cable area with a current density that was too low (i.e., 1 A/m²)* to effectuate efficient corrosion. The accelerated corrosion cell presented here takes advantage of the restricted area within the corrosion flow cell to maximize the corrosion rate in a consistent and calibrated manner (i.e., 2,000 A/m²).
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Alam, Todd Michael, and Sarah K. McIntyre. Development of a micro flow-through cell for high field NMR spectroscopy. Office of Scientific and Technical Information (OSTI), 2011. http://dx.doi.org/10.2172/1018472.

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Kumar, Rajan. Microfluidic Flow Retardation Device for Tagless Cancer Cell Analysis for Metastatic Potential. Defense Technical Information Center, 2012. http://dx.doi.org/10.21236/ada566934.

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