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Journal articles on the topic 'Flow cytometers'

Author: Grafiati

Published: 6 September 2023

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1

Chao, Zixi, Yong Han, Zeheng Jiao, Zheng You, and Jingjing Zhao. "Prism Design for Spectral Flow Cytometry." Micromachines 14, no. 2 (2023): 315. http://dx.doi.org/10.3390/mi14020315.

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Flow cytometers are instruments used for the rapid quantitative analysis of cell suspension. Traditional flow cytometry uses multi-channel filters to detect fluorescence, whereas full-spectrum fluorescence based on dispersion detection is a more effective and accurate method. The application of various dispersion schemes in flow cytometry spectroscopy has been studied. From the perspective of modern detectors and demand for the miniaturization of flow cytometry, prism dispersion exhibits higher and more uniform light energy utilization, meaning that it is a more suitable dispersion method for

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2

Volotovski, I. D., and S. V. Pinchuk. "Flow cytometry. Basics of technology and its application in biology." Proceedings of the National Academy of Sciences of Belarus, Biological Series 67, no. 2 (2022): 229–42. http://dx.doi.org/10.29235/1029-8940-2022-67-2-229-242.

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The given review is an attempt of concentrated consideration of flow cytometry problem which is widely used as a fundamental research approach in various fields of biology like cell biology, biophysics, biochemistry and molecular biology and also in applied and diagnostic medicine. The method principle, construction of flow cytometers and their possibilities (study of structure and function state of cell populations and cell sorting), usage of lasers in flow cytometers, wide assortment of fluorophores and monoclonal antibodies. The concrete examples of flow cytometer methods in different exper

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Cottingham, Katie. "Incredible shrinking flow cytometers." Analytical Chemistry 77, no. 3 (2005): 73 A—76 A. http://dx.doi.org/10.1021/ac053330g.

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Anderson, April, and Jolene Bradford. "Rare Event detection using Acoustic Cytometry (144.4)." Journal of Immunology 184, no. 1_Supplement (2010): 144.4. http://dx.doi.org/10.4049/jimmunol.184.supp.144.4.

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Abstract Detection of rare events includes populations comprising less than 5% of the total cells, which involves the detection of stem cells, minimal residual disease and fetomaternal hemorrhage. Analysis of rare cell populations requires the collection of high numbers of events in order to attain a reliable measure of accuracy, leading to long acquisition times. Acoustic cytometry is a new technology which allows dilute samples to be processed quickly. We compared collection times of the Applied Biosystems® Attune™ Acoustic Focusing Cytometer to conventional cytometers. Varying numbers of CD

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5

Shrirao, Anil B., Zachary Fritz, Eric M. Novik, et al. "Microfluidic flow cytometry: The role of microfabrication methodologies, performance and functional specification." TECHNOLOGY 06, no. 01 (2018): 1–23. http://dx.doi.org/10.1142/s2339547818300019.

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Flow cytometry is an invaluable tool utilized in modern biomedical research and clinical applications requiring high throughput, high resolution particle analysis for cytometric characterization and/or sorting of cells and particles as well as for analyzing results from immunocytometric assays. In recent years, research has focused on developing microfluidic flow cytometers with the motivation of creating smaller, less expensive, simpler, and more autonomous alternatives to conventional flow cytometers. These devices could ideally be highly portable, easy to operate without extensive user trai

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Shapiro, Howard M., and Nancy G. Perlmutter. "Personal cytometers: Slow flow or no flow?" Cytometry Part A 69A, no. 7 (2006): 620–30. http://dx.doi.org/10.1002/cyto.a.20284.

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7

NOGUCHI, Yoshio, and Yoshio TENJIN. "Laser flow cytometers newly developed." Review of Laser Engineering 15, no. 8 (1987): 647–56. http://dx.doi.org/10.2184/lsj.15.647.

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8

Nitta, Carolina Franco, Mackenzie Pierce, Jeanne Elia, et al. "A rapid and high-throughput T cell immunophenotyping assay for cellular therapy bioprocess using the Cellaca PLX image cytometer." Journal of Immunology 210, no. 1_Supplement (2023): 250.06. http://dx.doi.org/10.4049/jimmunol.210.supp.250.06.

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Abstract Cellular therapies such as chimeric antigen receptor (CAR) T or NK cells undergo phenotypic characterization during discovery and development of novel therapies, biomanufacturing, and upstream patient cell processing for clinical use. Samples are routinely analyzed via flow cytometry for T cell, NK cell, B cell, and monocyte surface marker expression. Image cytometry systems perform cell-based assays that can be used as a convenient alternative to flow cytometry. Recently, a new high-throughput image cytometer, the Cellaca PLX system (Nexcelom, Lawrence, MA) was developed for immunoph

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Kotz, Kenneth T., Anne C. Petrofsky, Ramin Haghgooie, Robert Granier, Mehmet Toner, and Ronald G. Tompkins. "Inertial focusing cytometer with integrated optics for particle characterization." TECHNOLOGY 01, no. 01 (2013): 27–36. http://dx.doi.org/10.1142/s233954781350009x.

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Microfluidic inertial focusing has been shown as a simple and effective method to localize cells and particles within a flow cell for interrogation by an external optical system. To enable portable point of care optical cytometry, however, requires a reduction in the complexity of the large optical systems that are used in standard flow cytometers. Here, we present a new design that incorporates optical waveguides and focusing elements with an inertial focusing flow cell to make a compact robust cytometer capable of enumerating and discriminating beads, cells, and platelets.

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Bang, Hyunwoo, Hoyoung Yun, Won Gu Lee, et al. "Expansion channel for microchip flow cytometers." Lab on a Chip 6, no. 10 (2006): 1381. http://dx.doi.org/10.1039/b604578b.

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11

Steen, Harald B., and Trond Stokke. "Dye exclusion artifact in flow cytometers." Cytometry 47, no. 3 (2002): 200–205. http://dx.doi.org/10.1002/cyto.10063.

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12

Bernander, Rolf, Trond Stokke, and Erik Boye. "Flow cytometry of bacterial cells: Comparison between different flow cytometers and different DNA stains." Cytometry 31, no. 1 (1998): 29–36. http://dx.doi.org/10.1002/(sici)1097-0320(19980101)31:1<29::aid-cyto4>3.0.co;2-e.

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13

Guenther, Garret, and William Telford. "Comparison of flow cytometers using the side population technique to identify stem cells (TECH2P.900)." Journal of Immunology 194, no. 1_Supplement (2015): 206.10. http://dx.doi.org/10.4049/jimmunol.194.supp.206.10.

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Abstract Traditional methods using the side population analysis to identify stem cells involved the use of a flow cytometer with an ultra violet laser excited dye. This presents some challenges for researchers in relation to costs, and even though other dyes such as DyeCycle Violet (DCV) are now available that use the more economical violet laser, the cost of a flow cytometer to provide this type of analysis is still an obstacle. More recently, new benchtop flow cytometers have become available that can provide the quality of a high-end flow cytometer at a much more affordable cost. In this an

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14

Bradford, Jolene A., Justin Meskas, and Patricia Sardina. "Evaluation of Cytometer Sensitivity and Stability using Automated Analysis." Journal of Immunology 202, no. 1_Supplement (2019): 131.41. http://dx.doi.org/10.4049/jimmunol.202.supp.131.41.

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Abstract Flow cytometers are standard tools of the immunologist, providing simultaneous measurement of many targets. The performance of a flow cytometer may vary between instruments, or over time within the same instrument. Evaluation of cytometer performance is essential to ensure results are consistent over time within the same instrument for longitudinal studies and day-to-day data consistency, and across multiple instruments when more than one cytometer is being utilized. Basic capabilities of each fluorescent detector can be estimated using the Q & B calculations, where Q is detection

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15

Zhang, Ting, Mengge Gao, Xiao Chen, et al. "Demands and technical developments of clinical flow cytometry with emphasis in quantitative, spectral, and imaging capabilities." Nanotechnology and Precision Engineering 5, no. 4 (2022): 045002. http://dx.doi.org/10.1063/10.0015301.

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As the gold-standard method for single-cell analysis, flow cytometry enables high-throughput and multiple-parameter characterization of individual biological cells. This review highlights the demands for clinical flow cytometry in laboratory hematology (e.g., diagnoses of minimal residual disease and various types of leukemia), summarizes state-of-the-art clinical flow cytometers (e.g., FACSLyricTM by Becton Dickinson, DxFLEX by Beckman Coulter), then considers innovative technical improvements in flow cytometry (including quantitative, spectral, and imaging approaches) to address the limitati

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16

Yang, Ruey-Jen, Lung-Ming Fu, and Hui-Hsiung Hou. "Review and perspectives on microfluidic flow cytometers." Sensors and Actuators B: Chemical 266 (August 2018): 26–45. http://dx.doi.org/10.1016/j.snb.2018.03.091.

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17

Steen, Harald B. "A sample injection device for flow cytometers." Cytometry 49, no. 2 (2002): 70–72. http://dx.doi.org/10.1002/cyto.10147.

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18

Steen, Harald B. "Noise, Sensitivity, and Resolution of Flow Cytometers." Cytometry 13, no. 8 (1992): 822–30. http://dx.doi.org/10.1002/cyto.990130804.

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19

Kaleem, Zahid. "Flow Cytometric Analysis of Lymphomas: Current Status and Usefulness." Archives of Pathology & Laboratory Medicine 130, no. 12 (2006): 1850–58. http://dx.doi.org/10.5858/2006-130-1850-fcaolc.

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Abstract Context.—Immunophenotyping has become a routine practice in the diagnosis and classification of most cases of non-Hodgkin lymphoma, and flow cytometry is often the method of choice in many laboratories. The role that flow cytometry plays, however, extends beyond just diagnosis and classification. Objective.—To review and evaluate the current roles of flow cytometry in non-Hodgkin lymphoma, to compare it with immunohistochemistry, and to discuss its potential future applications in the molecular diagnostic era. Data Sources.—The information contained herein is derived from peer-reviewe

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20

Hyrkas, Jeremy, Daniel Halperin, and Bill Howe. "Time-Varying Clusters in Large-Scale Flow Cytometry." Proceedings of the AAAI Conference on Artificial Intelligence 29, no. 2 (2015): 4022–23. http://dx.doi.org/10.1609/aaai.v29i2.19067.

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Flow cytometers measure the optical properties of particles to classify microbes. Recent innovations have allowed oceanographers to collect flow cytometry data continuously during research cruises, leading to an explosion of data and new challenges for the classification task. The massive scale, time-varying underlying populations, and noisy measurements motivate the development of new classification methods. We describe the problem, the data, and some preliminary results demonstrating the difficulty with conventional methods.

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21

Lucchetti, Donatella, Alessandra Battaglia, Claudio Ricciardi-Tenore, et al. "Measuring Extracellular Vesicles by Conventional Flow Cytometry: Dream or Reality?" International Journal of Molecular Sciences 21, no. 17 (2020): 6257. http://dx.doi.org/10.3390/ijms21176257.

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Intense research is being conducted using flow cytometers available in clinically oriented laboratories to assess extracellular vesicles (EVs) surface cargo in a variety of diseases. Using EVs of various sizes purified from the HT29 human colorectal adenocarcinoma cell line, we report on the difficulty to assess small and medium sized EVs by conventional flow cytometer that combines light side scatter off a 405 nm laser with the fluorescent signal from the EVs general labels Calcein-green and Calcein-violet, and surface markers. Small sized EVs (~70 nm) immunophenotyping failed, consistent wit

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22

Pugsley, Haley Renee, and Alexandra G. Sutton. "Analysis of STAT Protein Phosphorylation in T Cells, NK Cells, and B Cells Using High Sensitivity Flow Cytometry." Journal of Immunology 210, no. 1_Supplement (2023): 168.10. http://dx.doi.org/10.4049/jimmunol.210.supp.168.10.

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Abstract In the immune system, cytokine-induced protein phosphorylation is a key cell signaling event that modulates diverse functions, including proliferation, differentiation, apoptosis, and migration. The degree of protein phosphorylation is determined by both the cytokine concentration and the number of cytokine-specific receptors on the cell surface, which are differentially expressed across immune cell populations. Signal transducer and activator of transcription (STAT) proteins are transcription factors that translocate to the nucleus upon activation by tyrosine phosphorylation and alte

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23

de Rutte, Joseph, Robert Dimatteo, Sheldon Zhu, Maani M. Archang, and Dino Di Carlo. "Sorting single-cell microcarriers using commercial flow cytometers." SLAS Technology 27, no. 2 (2022): 150–59. http://dx.doi.org/10.1016/j.slast.2021.10.004.

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24

Wood, James C. S., and Robert A. Hoffman. "Evaluating fluorescence sensitivity on flow cytometers: An overview." Cytometry 33, no. 2 (1998): 256–59. http://dx.doi.org/10.1002/(sici)1097-0320(19981001)33:2<256::aid-cyto22>3.0.co;2-s.

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25

deMello, Andrew, Anand Rane, Gregor Holzner, and Stavros Stavrakis. "Ultra-High-Throughput Multi-Parametric Imaging Flow Cytometry." EPJ Web of Conferences 215 (2019): 10001. http://dx.doi.org/10.1051/epjconf/201921510001.

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I will present a microfluidic imaging flow cytometer incorporating stroboscopic illumination, for blur-free cellular analysis at throughputs exceeding 100,000 cells per second. By combining passive (inertial or viscoelastic) focusing of cells in parallel microchannels with stroboscopic illumination, such chip-based cytometers are able to extract multi-colour fluorescence and bright-field images of single cells moving at high linear velocities. This in turn allows accurate sizing of individual cells, intracellular localization and analysis of heterogeneous cell suspensions. The method is showca

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26

Lin, Bo, Leo Chan, Ning Lai, et al. "Obtain consistent and accurate PBMC cell counting results with Cellometer automatic cell counter (65.14)." Journal of Immunology 186, no. 1_Supplement (2011): 65.14. http://dx.doi.org/10.4049/jimmunol.186.supp.65.14.

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Abstract Traditional methods for fluorescence-based PBMC analysis commonly employ manual hemacytometer and standard flow cytometry for concentration and viability measurement. Manual cell counting requires trained technicians, which is time-consuming and prone to human error. In addition, PBMC samples are usually contaminated with RBCs and platelets, which may increase manual counting difficulty. Standard flow cytometry remains expensive, large in size, and require considerable amount of maintenance, which may not be ideal for research laboratories that need a cost-effective method. In additio

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27

Smith, R. W. "Non-Imaging Microscopies: Flow Cytometry as a Correlative Analytical Tool in the Quantification of Cell Structure, Autofluorescence, Fluorescent Probes and Cell Populations." Microscopy and Microanalysis 5, S2 (1999): 490–91. http://dx.doi.org/10.1017/s1431927600015774.

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Non-imaging microscopy has developed somewhat independently of both traditional light microscopy and laser confocal microscopy. Flow cytometry is the chief commercial and research technology among these microscopies, and, like other nonimaging detection systems, developed around the theme of automation in clinical laboratory medicine. It is an important correlative or parallel microscopy to several image forming microscopical methods. Cell sorting is an important option as well.The basic structure of the flow cytometer certainly parallels light, laser and electron microscopes. The flow cytomet

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Chaturvedi, Akhil, and Sai Siva Gorthi. "Automated Blood Sample Preparation Unit (ABSPU) for Portable Microfluidic Flow Cytometry." SLAS TECHNOLOGY: Translating Life Sciences Innovation 22, no. 1 (2016): 73–80. http://dx.doi.org/10.1177/2211068216663604.

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Portable microfluidic diagnostic devices, including flow cytometers, are being developed for point-of-care settings, especially in conjunction with inexpensive imaging devices such as mobile phone cameras. However, two pervasive drawbacks of these have been the lack of automated sample preparation processes and cells settling out of sample suspensions, leading to inaccurate results. We report an automated blood sample preparation unit (ABSPU) to prevent blood samples from settling in a reservoir during loading of samples in flow cytometers. This apparatus automates the preanalytical steps of d

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29

Miyake, Ryo, Hiroshi Ohki, and Isao Yamazaki. "Investigation of Sheath Flow Chambers for Flow Cytometers. 1st Report, Stabilization of Sheath Flow." Transactions of the Japan Society of Mechanical Engineers Series B 61, no. 591 (1995): 4039–45. http://dx.doi.org/10.1299/kikaib.61.4039.

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30

Rogers, Clare, Melanie Gubbels Bupp, Unyoung Kim, et al. "Successful integration of practical flow cytometric experience into undergraduate education. (51.4)." Journal of Immunology 186, no. 1_Supplement (2011): 51.4. http://dx.doi.org/10.4049/jimmunol.186.supp.51.4.

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Abstract Flow cytometry is an essential tool in almost every discipline of cell biology, and is increasingly utilized in a variety of other associated yet diverse fields, including molecular biology, bioengineering, microbiology and marine biology. For many years, access to the use of flow cytometers as teaching tools, even in large universities, was limited to graduate programs, due to the cost of instrument purchase and maintenance, and to the complexity of operation. Recently, several affordable, bench-top flow cytometers have appeared on the market. The affordability and user-friendly natu

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31

Edwards, Bruce S., and Larry A. Sklar. "Flow Cytometry." Journal of Biomolecular Screening 20, no. 6 (2015): 689–707. http://dx.doi.org/10.1177/1087057115578273.

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Modern flow cytometers can make optical measurements of 10 or more parameters per cell at tens of thousands of cells per second and more than five orders of magnitude dynamic range. Although flow cytometry is used in most drug discovery stages, “sip-and-spit” sampling technology has restricted it to low-sample-throughput applications. The advent of HyperCyt sampling technology has recently made possible primary screening applications in which tens of thousands of compounds are analyzed per day. Target-multiplexing methodologies in combination with extended multiparameter analyses enable profil

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32

BROWN, MICHAEL C., ROBERT A. HOFFMAN, and STEFAN J. KIRCHANSKI. "Controls for Flow Cytometers in Hematology and Cellular Immunology." Annals of the New York Academy of Sciences 468, no. 1 Clinical Cyto (1986): 93–103. http://dx.doi.org/10.1111/j.1749-6632.1986.tb42032.x.

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33

PETEGEM, M., R. CARTUYVELS, P. SCHOUWER, V. DUPPEN, W. GOOSSENS, and L. HOVE. "Comparative evaluation of three flow cytometers for reticulocyte enumeration." Clinical & Laboratory Haematology 15, no. 2 (2008): 103–11. http://dx.doi.org/10.1111/j.1365-2257.1993.tb00133.x.

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34

Wang, Jun Sheng, You Nan Song, Jin Yang Sun та ін. "A Microfluidic Cytometer for Quantitative Evaluation of Radiation Dose by γ-H2AX". Applied Mechanics and Materials 522-524 (лютий 2014): 1119–22. http://dx.doi.org/10.4028/www.scientific.net/amm.522-524.1119.

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Evaluation of radiation dose is very important for the detection of radiation damage. γ-H2AX is a popular biological dosimeter to evaluate the radiation effect. Typically, bulky and expensive commercial flow cytometers are used to detect γ-H2AX. This paper presents a miniaturized and highly sensitive cytometer using a microfluidic chip for evaluating the radiation dose by detecting the mean immunofluorescence intensity of γ-H2AX. A compact optical focusing system and a shift-phase differential amplifier are designed to improve the detection sensitivity. Sample lymphocyte cells are stained by F

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Sukovich, David J., Samuel C. Kim, Noorsher Ahmed, and Adam R. Abate. "Bulk double emulsification for flow cytometric analysis of microfluidic droplets." Analyst 142, no. 24 (2017): 4618–22. http://dx.doi.org/10.1039/c7an01695f.

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Pugsley, Haley Renee, Bryan R. Davidson, and Philip Morrissey. "Immunophenotyping extracellular vesicles using the CellStream flow cytometer." Journal of Immunology 202, no. 1_Supplement (2019): 131.5. http://dx.doi.org/10.4049/jimmunol.202.supp.131.5.

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Abstract Extracellular vesicles are membrane derived structures that include exosomes, microvesicles and apoptotic bodies. The importance of extracellular vesicles as key mediators of intercellular communication is not well understood. Exosomes have been shown to transfer molecules between cells, potentially transmitting signals. Exosomes are released under normal physiological conditions; however, they are also believed to serve as mediators in the pathogenesis of neurological, vascular, haematological and autoimmune diseases as well as cancer. Quantifying and characterizing extracellular ves

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37

WANG, XIANWEN, FENG CHEN, ZHI CHENG, YAOHUA DU, and TAIHU WU. "AUTOMATED GATING OF PORTABLE CYTOMETER DATA BASED ON SKEW t MIXTURE MODELS." Journal of Mechanics in Medicine and Biology 15, no. 03 (2015): 1550033. http://dx.doi.org/10.1142/s0219519415500335.

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A major component of flow cytometry (FCM) data analysis involves gating, which is the process of identifying homogeneous groups of cells. With the rapid development of the portable flow cytometer, manual gating techniques have been unable to meet the demand for accurate and rapid analysis of samples. To provide a practical application for portable devices, we propose a flexible, statistical model-based clustering approach for identifying cell populations in FCM data. This approach, which mimics the manual gating process, employs a finite mixture model with a density function of skew t distribu

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de Bruijn, Douwe S., Koen F. A. Jorissen, Wouter Olthuis, and Albert van den Berg. "Determining Particle Size and Position in a Coplanar Electrode Setup Using Measured Opacity for Microfluidic Cytometry." Biosensors 11, no. 10 (2021): 353. http://dx.doi.org/10.3390/bios11100353.

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Microfluidic impedance flow cytometers enable high-throughput, non-invasive, and label-free detection of single-cells. Cytometers with coplanar electrodes are easy and cheap to fabricate, but are sensitive to positional differences of passing particles, owing to the inhomogeneous electric field. We present a novel particle height compensation method, which employs the dependence of measured electrical opacity on particle height. The measured electrical opacity correlates with the particle height as a result of the constant electrical double layer series capacitance of the electrodes. As an alt

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Lee, Gwo-Bin, Che-Hsin Lin, and Guan-Liang Chang. "Micro flow cytometers with buried SU-8/SOG optical waveguides." Sensors and Actuators A: Physical 103, no. 1-2 (2003): 165–70. http://dx.doi.org/10.1016/s0924-4247(02)00305-9.

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Tsai, Chien-Hsiung, Hui-Hsiung Hou, and Lung-Ming Fu. "An optimal three-dimensional focusing technique for micro-flow cytometers." Microfluidics and Nanofluidics 5, no. 6 (2008): 827–36. http://dx.doi.org/10.1007/s10404-008-0284-6.

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MIYAKE, Ryo, Hiroshi OHKI, Isao YAMAZAKI, and Takeo TAKAGI. "Investigation of Sheath Flow Chambers for Flow Cytometers (Micro Machined Flow Chamber with Low Pressure Loss)." JSME International Journal Series B 40, no. 1 (1997): 106–13. http://dx.doi.org/10.1299/jsmeb.40.106.

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Ding, Mei, Roger Clark, Catherine Bardelle, et al. "Application of High-Throughput Flow Cytometry in Early Drug Discovery: An AstraZeneca Perspective." SLAS DISCOVERY: Advancing the Science of Drug Discovery 23, no. 7 (2018): 719–31. http://dx.doi.org/10.1177/2472555218775074.

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Flow cytometry is a powerful tool providing multiparametric analysis of single cells or particles. The introduction of faster plate-based sampling technologies on flow cytometers has transformed the technology into one that has become attractive for higher throughput drug discovery screening. This article describes AstraZeneca’s perspectives on the deployment and application of high-throughput flow cytometry (HTFC) platforms for small-molecule high-throughput screening (HTS), structure–activity relationship (SAR) and phenotypic screening, and antibody screening. We describe the overarching HTF

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43

Shapiro, Howard M., and Howard M. Shapiro. "Gently Down The Stream: Flow Cytometry As Microscopy." Microscopy and Microanalysis 7, S2 (2001): 610–11. http://dx.doi.org/10.1017/s1431927600029123.

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Flow cytometry is an analytical technique in which optical measurements are made of cells or other biological particles as the cells or particles flow, ideally in single file, in a fluid stream past one or more optical measurement stations. Modern optical flow cytometers typically measure light scattered at small (1-5°) and large (15°-135°) angles to an illuminating laser beam, and fluorescence emitted in three or more discrete spectral bands; the most complex instruments employ three or four spatially separated illuminating beams at different wavelengths and can measure twelve fluorescence si

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Welsh, Joshua, Julia Kepley, Ariel Rosner, Peter Horak, Jay Berzofsky, and Jennifer Jones. "Prospective Use of High-Refractive Index Materials for Single Molecule Detection in Flow Cytometry." Sensors 18, no. 8 (2018): 2461. http://dx.doi.org/10.3390/s18082461.

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Phenotyping extracellular vesicles (EVs), where surface receptor expression is often as low as one molecule per EV, remains problematic due to the inability of commercial flow cytometers to provide single-fluorescent molecule sensitivity. While EVs are widely considered to be of great potential as diagnostic, prognostic and theranostic biomarkers, their use is currently hindered by the lack of tools available to accurately and reproducibly enumerate and phenotype them. Herein, we propose a new class of labels that leverage the biophysical properties of materials with unique complex refractive

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Lin, Che-Hsin, and Gwo-Bin Lee. "Micromachined flow cytometers with embedded etched optic fibers for optical detection." Journal of Micromechanics and Microengineering 13, no. 3 (2003): 447–53. http://dx.doi.org/10.1088/0960-1317/13/3/315.

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46

Rahman, Md Mizanur, Susane Giti, and Debashish Saha. "Flow Cytomerty: Clinical Applications in Haemato-Oncology." Journal of Armed Forces Medical College, Bangladesh 11, no. 1 (2016): 74–80. http://dx.doi.org/10.3329/jafmc.v11i1.30677.

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In the past decade, the use of flow cytometry in the clinical haematology laboratory has grown substantially due to the development of smaller, user-friendly, less-expensive instruments and a continuous increase in the number of clinical applications. Multiple characteristics of single cells can be analyzed rapidly by flow cytometry. Both qualitative and quantitative information are obtained by flow cytometry whereas previously only in research institutions and esteemed academic centres flow cytometers were found. With advances in technology now it is possible for secondary level hospitals to

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47

MIYAKE, Ryo, Hiroshi OHKI, Isao YAMAZAKI, and Takeo TAKAGI. "Investigation of Sheath Flow Chambers for Flow Cytometers. 2nd Report. Micro machined Flow Chamber with Low Pressure Loss." Transactions of the Japan Society of Mechanical Engineers Series B 62, no. 597 (1996): 1693–99. http://dx.doi.org/10.1299/kikaib.62.1693.

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48

Janossy, George, Ilesh V. Jani, Nicholas J. Bradley, Arsene Bikoue, Tim Pitfield, and Debbie K. Glencross. "Affordable CD4+-T-Cell Counting by Flow Cytometry: CD45 Gating for Volumetric Analysis." Clinical and Vaccine Immunology 9, no. 5 (2002): 1085–94. http://dx.doi.org/10.1128/cdli.9.5.1085-1094.2002.

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ABSTRACT The flow cytometers that are currently supported by industry provide accurate CD4+-T-cell counts for monitoring human immunodeficiency virus disease but remain unaffordable for routine service work under resource-poor conditions. We therefore combined volumetric flow cytometry (measuring absolute lymphocyte counts in unit volumes of blood) and simpler protocols with generic monoclonal antibodies (MAbs) to increase cost efficiency. Volumetric absolute counts were generated using CD45/CD4 and CD45/CD8 MAb combinations in two parallel tubes. The percentage values for the various subsets

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Mulama, David, Roman J. Riveria, Kimberly McKinney, et al. "Abstract 3053: Comparative validation of neuroblastoma cell lines using flow cytometry and CyToF." Cancer Research 83, no. 7_Supplement (2023): 3053. http://dx.doi.org/10.1158/1538-7445.am2023-3053.

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Abstract Introduction The validation of patient derived cell lines and other biologics has gained importance due to more frequent laboratory collaborations. Polychromatic flow cytometry has been the gold standard for detection and analysis of biochemical, structural complexity and characterization of cellular particles in biological systems. Despite the tremendous advancement in flow cytometry from the earlier simple, slow version of 1960s to more recent complex, fast spectral analyzers with comprehensive structural and functional immune profiling capability, this development has not matched t

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Botha, Jaco, Haley R. Pugsley, and Aase Handberg. "Conventional, High-Resolution and Imaging Flow Cytometry: Benchmarking Performance in Characterisation of Extracellular Vesicles." Biomedicines 9, no. 2 (2021): 124. http://dx.doi.org/10.3390/biomedicines9020124.

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Flow cytometry remains a commonly used methodology due to its ability to characterise multiple parameters on single particles in a high-throughput manner. In order to address limitations with lacking sensitivity of conventional flow cytometry to characterise extracellular vesicles (EVs), novel, highly sensitive platforms, such as high-resolution and imaging flow cytometers, have been developed. We provided comparative benchmarks of a conventional FACS Aria III, a high-resolution Apogee A60 Micro-PLUS and the ImageStream X Mk II imaging flow cytometry platform. Nanospheres were used to systemat

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