Academic literature on the topic 'Fluorescence'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Fluorescence.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Fluorescence"

1

Sona, B. Warrier and Prashant S. Kharkar*. "DESIGN, SYNTHESIS AND CHARACTERIZATION OF FLUORESCENT PROBES BASED ON SCHIFF BASES." Indo American Journal of Pharmaceutical Sciences 04, no. 06 (June 21, 2017): 1505–10. https://doi.org/10.5281/zenodo.815365.

Full text
Abstract:
Abstract: Schiff bases of fluorescein were synthesized from fluorescein hydrazide using different aromatic aldehydes. These compounds show bright fluorescence different from that of starting material, fluorescein. The compounds were characterised by spectroscopic methods. Absorption and fluorescence spectra of the compounds were also recorded. Keywords: Fluorescein, Schiff bases, Fluorescein hydrazide, fluorescent probes, fluorescence
APA, Harvard, Vancouver, ISO, and other styles
2

MATSUMOTO, TAKURO, ATSUSHI SUETSUGU, KOSUKE HASEGAWA, MIKI NAKAMURA, YUHEI SHIBATA, HITOMI AOKI, TAKAHIRO KUNISADA, et al. "A Mouse Model of Fluorescent Protein-expressing Disseminated Peritoneal Lymphoma for Fluorescence-guided Surgery." Anticancer Research 36, no. 9 (September 9, 2016): 4483–88. http://dx.doi.org/10.21873/anticanres.10993.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Pawley, James B. "Fluorescence Microscopy and Fluorescence Probes." Microscopy and Microanalysis 4, no. 2 (April 1998): 164–65. http://dx.doi.org/10.1017/s1431927698000166.

Full text
Abstract:
Fluorescence Microscopy and Fluorescence Probes, edited by Jan Slavik, 1996. Plenum Press, New York and London. 306 pages. (hardback, $95)This volume is a compilation of abstracts of papers presented at the Fluorescence Microscopy and Fluorescence Probes Conference held in Prague in June 1995. This conference was one of the first tangible results of the end of the Cold War in the field of microscopy. As such, it attracted a great many of those who constitute the vanguard of progress in the field of fluorescence microscopy, on both sides of the old East-West divide. About one-third of the 127 contributors were from the West.
APA, Harvard, Vancouver, ISO, and other styles
4

Sessions, S. K. "Fluorescence Microscopy; Quantitative Fluorescence Microscopy." Systematic Biology 42, no. 2 (June 1, 1993): 224–25. http://dx.doi.org/10.1093/sysbio/42.2.224.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Heldt, Józef, Janina R. Heldt, and Jerzy Kamiński. "Steady-state and Time-resolved Spectroscopic Studies of Benzanilides." Zeitschrift für Naturforschung A 54, no. 8-9 (September 1, 1999): 495–502. http://dx.doi.org/10.1515/zna-1999-8-909.

Full text
Abstract:
Steady-state and time-resolved spectroscopic studies of benzanilide (I) and jV-methylbenzanilide (II)were performed at 298 and 77 K in various solvents. The results indicate that benzanilide fluorescencein non-polar solvents at room temperature involves three independent modes of emission: F1 (LE) normalfluorescence from the initially excited state S1 (LE) with λmax = 320 nm, F2´(PT) fluorescence from the proton transfer tautomer with λmax = 468 nm, F2″CT) fluorescence from the species where intramolecular charge transfer appears, with λmax = 510 nm. At 77 K in MCH a new fluorescence band, Fag, appears at λmax=415 nm instead of the F2(PT) and F2″CT) fluorescence. This new emission originates from benzanilide dipolar aggregates or cis-imidol dimers. The decay times of these emission modes aredifferent.N-methylbenzanilide, dissolved in nonpopular and weakly polar solvents at room temperature and at77 K, shows only two fluorescence modes, i.e., the normal and the charge-transfer emissions at 320 nmand 520 nm, respectively. The fluorescence is deactivated with two decay times, 30 ps and 2.05 ns, inMCH solution.
APA, Harvard, Vancouver, ISO, and other styles
6

Ravinson, Daniel Sylvinson Muthiah, and Mark E. Thompson. "Thermally assisted delayed fluorescence (TADF): fluorescence delayed is fluorescence denied." Materials Horizons 7, no. 5 (2020): 1210–17. http://dx.doi.org/10.1039/d0mh00276c.

Full text
Abstract:
Thermally assisted delayed fluorescence (TADF) allows for efficient collection of both singlet and triplet excitons with both emitting through the singlet channel. TADF opens the door to photo- and electroluminescence efficiencies close to 100%.
APA, Harvard, Vancouver, ISO, and other styles
7

Ma, Xiben, Jimeng Cheng, Sijun Fan, Xin Wang, Wei Chen, Shubin Chen, and Lili Hu. "Silver-Neodymium Codoped Lithium Aluminum Metaphosphate Glasses for Radio-Photoluminescence Dosimeter." Materials 15, no. 16 (August 11, 2022): 5527. http://dx.doi.org/10.3390/ma15165527.

Full text
Abstract:
Commercial radio-photoluminescence (RPL) glass dosimeters generally use Ag single-doped phosphate glass as a single-wavelength sensor. Now, a novel type of Ag–Nd-codoped phosphate glass has been developed, which can be applied to dual-wavelength or multi-wavelength RPL sensors, and can thus improve the accuracy and stability of RPL dosimeters. An anhydrous 99.5 (0.7LiPO3–0.3Al (PO3)3) −0.25Ag2O–0.25Nd2O3 glass was prepared and irradiated at different doses, and then the absorption, fluorescence, infrared transmission spectra, as well as fluorescence lifetimes were tested and analyzed. The results show that there is an energy transfer between the Ag defect center and Nd3+ ions, and the transfer efficiency using 380 nm excitation is greater than that using 310 nm excitation. Aside from the 650 nm fluorescence of the Ag defect center, strong 882 nm and 1054 nm fluorescences of Nd ions are exhibited. It is possible that these fluorescences would allow the developed Ag–Nd-codoped phosphate glass to be applied to new RPL glass sensors and dosimeters.
APA, Harvard, Vancouver, ISO, and other styles
8

BEARDER, E. ARTHUR. "Fluorescence." Journal of the Society of Dyers and Colourists 27, no. 12 (October 22, 2008): 270–79. http://dx.doi.org/10.1111/j.1478-4408.1911.tb00530.x.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Inoue, S., O. Shimomura, M. Goda, M. Shribak, and P. T. Tran. "Fluorescence polarization of green fluorescence protein." Proceedings of the National Academy of Sciences 99, no. 7 (April 2, 2002): 4272–77. http://dx.doi.org/10.1073/pnas.062065199.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Gardner, Craig M., Steven L. Jacques, and Ashley J. Welch. "Fluorescence spectroscopy of tissue: recovery of intrinsic fluorescence from measured fluorescence." Applied Optics 35, no. 10 (April 1, 1996): 1780. http://dx.doi.org/10.1364/ao.35.001780.

Full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "Fluorescence"

1

Nagl, Stefan. "Fluorescent multiple chemical sensing using time-domain fluorescence lifetime imaging." kostenfrei, 2008. http://www.opus-bayern.de/uni-regensburg/volltexte/2008/996/.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Remy, Charlotte. "Synthèse et étude de récepteurs moléculaires fluorescents pour la détection de molécules neutres." Thesis, Université Paris-Saclay (ComUE), 2016. http://www.theses.fr/2016SACLN070/document.

Full text
Abstract:
La détection de molécules toxiques pour l’Homme et son environnement est d’une importance cruciale et fait partie des préoccupations majeures de la société actuelle. Les résidus de pesticides tels que l’atrazine ainsi que la mélamine font notamment partie de ces molécules dangereuses pour la santé humaine. Ces deux molécules sont principalement dosées par des techniques lourdes et coûteuses comme la spectrométrie de masse, la chromatographie ou l’électrochimie. De même, la détection des amines biogéniques représente un intérêt sociétal. Elles sont produites par des bactéries durant la décarboxylation des acides aminés dans les cellules. Leur détection permet ainsi d’évaluer la contamination microbiologique et la dégradation potentielle d’un aliment. Elles sont aujourd’hui dosées par chromatographie en phase liquide ou en en phase gaz, par électrochromatographie capillaire et par spectroscopie UV-visible. Quelques exemples de détection par fluorescence ont déjà été décrits dans la littérature mais la nécessité de développer de nouveaux récepteurs fluorescents efficaces est bien réelle.La fluorescence est une technique qui offre de multiples avantages tels que la sensibilité, la sélectivité et un faible coût. De nombreuses sondes fluorescentes capables de détecter des métaux lourds ont été développées au laboratoire PPSM. Cependant, la détection de molécules neutres par fluorescence représente un défi supplémentaire en raison de la nature plus faible de l’interaction, comparée à celle entre espèces chargées.La première étape de cette thèse a été de concevoir et de synthétiser un ensemble de sondes moléculaires fluorescentes, aussi bien pour la détection de l’atrazine, de ses produits de dégradation et des dérivés de la mélamine que pour la détection des amines biogéniques. Des fluorophores dérivés de la molécule de maléimide, de naphthalimide et de l’acide barbiturique ont ainsi été développés pour sonder les dérivés de triazine en exploitant leur système de trois liaisons hydrogène pour la reconnaissance moléculaire. De même, un calix[6]arène fluorescent a été conçu pour déceler la présence des amines biogéniques qui provoqueront une réponse fluorescente par encapsulation dans le calixarène.La deuxième étape a consisté à étudier les propriétés photophysiques de ces sondes. La sonde Naphth-AlcyneOMe possède un rendement quantique élevé, s’est révélée fortement solvatochrome. Elle est de plus sensible à la déprotonation de sa fonction imide. Des études RMN et de modélisation moléculaire ont également été menées afin de caractériser les sondes de manière plus approfondie et de comprendre plus précisément leur réactivité. La spectroscopie RMN a confirmé l’interaction par liaison hydrogène entre les sondes maléimide et naphthalimides et la molécule d’atrazine. Elle a aussi mis en évidence l’encapsulation de l’heptylamine dans le calix[6]arène. Pour sa part, la modélisation moléculaire nous a permis de mieux comprendre la photophysique de la sonde Naphth-TriazoleOMe.Enfin, la capacité des sondes à détecter les divers analytes cibles par fluorescence a été évaluée lors de la dernière étape de ce projet. La sonde TPA-BARB a présenté une forte exaltation de fluorescence en présence des dérivés de mélamine alors que le calix[6]arène-quinoléine Calix-Quino est capable de détecter les amines aliphatiques par fluorescence<br>The detection of molecules toxic for man and his environment is one of the major concerns of our society. Melamine and the pesticide residues such as atrazine are some of these dangerous molecules. These two molecules are usually measured with time-consuming and costly techniques like mass-spectrometry, chromatography or electrochemistry. In the same way, the detection of biogenic amines is of the greatest importance. They are produced by some bacteria during the decarboxylation of amino acids in the cells. So their detection allows to assess the microbiologic contamination and the potential degradation of a food. Today they are measured by chromatography in the liquid or gas phase, capillary electrochromatography and UV-visible spectroscopy. Some examples of detection by fluorescence have been described in scientific literature, but it is really necessary to develop some new efficient fluorescent receptors.Fluorescence is a technique which offers many advantages such as sensitivity, selectivity and a low cost. A lot of fluorescent probes able to detect heavy metals have been developed in PPSM laboratory. However the detection of neutral molecules by fluorescence represents an additional challenge as the interaction is weaker than with charged species.The first step of this thesis was to design and synthesize a set of fluorescent molecular probes designed to detect atrazine, the products of its degradation and melamine derivatives as well as biogenic amines. Some fluorophores based on maleimide, naphtalimide and barbituric acid moieties have been developed for the detection of the triazines derivatives by exploiting their three hydrogen bonds for molecular recognition. In order to detect the presence of biogenic amines, a fluorescent calix[6]arene which lead to a fluorescent change upon encapsulation in the calixarene cavity has been designed.The second step consisted in studying the photophysical properties of these probes. Naphth-AlcyneOMe probe which has a high quantum yield turned out to be highly solvatochromic. Moreover it is sensitive to the deprotonation of its imide function. NMR studies and molecular modeling were conducted in order to deepen the characteristics of the probes and better understand their reactivity. NMR spectroscopy confirmed the interaction through hydrogen bonding between maleimide and naphtalimide probes and the atrazine molecule.It highlighted the encapsulation of heptylamine in the calix[6]arene. Molecular modeling enabled us to better understand the photophysics of Naphth-TriazoleOMe probe.Finally the capacity of probes to detect the various analytes by fluorescence was assessed in our last part. TPA-BARB probe presented a high exaltation of fluorescence in presence of melamine derivatives whereas the calix[6]arène-quinoleine Calix-Quino is able to detect aliphatic amines by fluorescence
APA, Harvard, Vancouver, ISO, and other styles
3

Dennis, Allison Marie. "Quantum dot-fluorescent protein pairs as fluorescence resonance energy transfer pairs." Diss., Georgia Institute of Technology, 2009. http://hdl.handle.net/1853/37079.

Full text
Abstract:
Fluorescence resonance energy transfer (FRET)-based biosensors have been designed to fluorometrically detect everything from proteolytic activity to receptor-ligand interactions and structural changes in proteins. While a wide variety of fluorophores have demonstrated effectiveness in FRET probes, several potential sensor components are particularly notable. Semiconductor quantum dots (QDs) are attractive FRET donors because they are rather bright, exhibit high quantum yields, and their nanoparticulate structure enables the attachment of multiple acceptor molecules. Fluorescent proteins (FPs) are also of particular interest for fluorescent biosensors because design elements necessary for signal transduction, probe assembly, and device delivery and localization for intracellular applications can all be genetically incorporated into the FP polypeptide. The studies described in this thesis elucidate the important parameters for concerted QD-FP FRET probe design. Experimental results clarify issues of FRET pair selection, probe assembly, and donor-acceptor distance for the multivalent systems. Various analysis approaches are compared and guidelines asserted based on the results. To demonstrate the effectiveness of the QD-FP FRET probe platform, a ratiometric pH sensor is presented. The sensor, which uses the intrinsic pH-sensitivity of the FP mOrange to modulate the FP/QD emission ratio, exhibits a 20-fold change in its ratiometric measurement over a physiologically interesting pH range, making it a prime candidate for intracellular imaging applications.
APA, Harvard, Vancouver, ISO, and other styles
4

M'Baye, Gora Duportail Guy. "Sondes fluorescentes ratiométriques dérivées de la 3- Hydroxyflavone Etude spectroscopique de nouveaux dérivés et applications en biophysique membranaire /." Strasbourg : Université Louis Pasteur, 2007. http://eprints-scd-ulp.u-strasbg.fr:8080/755/01/MBAYE2007.pdf.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Henderson, Julius Nathan. "Crystallographic and spectroscopic studies of photoswitching in fluorescent proteins /." view abstract or download file of text, 2007. http://proquest.umi.com/pqdweb?did=1417810431&sid=5&Fmt=2&clientId=11238&RQT=309&VName=PQD.

Full text
Abstract:
Thesis (Ph. D.)--University of Oregon, 2007.<br>Typescript. Includes vita and abstract. Includes bibliographical references (leaves 143-151). Also available for download via the World Wide Web; free to University of Oregon users.
APA, Harvard, Vancouver, ISO, and other styles
6

Boujut, Margot. "Ligands Photo-Actifs pour l'imagerie de fluorescence du VEGFr." Thesis, Normandie, 2020. http://www.theses.fr/2020NORMR063.

Full text
Abstract:
Les VEGFr (Vascular Endothelium Growth Factor receptors, récepteurs des facteurs de croissance de l’endothélium vasculaire) sont des protéines responsables de l’angiogenèse, c’està-dire, la croissance des vaisseaux sanguins. De fait, ils sont impliqués dans les maladies liées à une vascularisation néfaste comme dans la croissance des tumeurs cancéreuses ou de néovascularisation rétinienne. Traiter ces vaisseaux sanguins sans atteindre les tissus sains est un enjeu qui nécessite de les imager spécifiquement et précisément, ces deux critères pouvant être atteints par imagerie de fluorescence. La spécificité en imagerie de fluorescence repose sur l’emploi d’une sonde, c’est-à-dire un fluorophore sélectif du phénomène à imager. Pour synthétiser des sondes sélectives, nous nous sommes inspirés d’un ligand connu du VEGFr : l’axitinib. La structure chimique de l’axitinib comporte un hétérocycle indazole avec deux rôles essentiels : (i) dans la fluorescence de l’axitinib, (ii) dans sa sélectivité pour le VEGFr. Cette structure a été modifiée de façon à introduire des substituants rendant la molécule plus fluorescente tout en conservant au mieux le squelette responsable de l’activité biologique. Une librairie d’une vingtaine de fluorophores a été synthétisée et étudiée pour des applications en imagerie de fluorescence<br>VEGFr (Vascular Endothelium Growth Factor receptors) are proteins responsible for the angiogenesis, meaning the growth of blood vessels. Consequently, they are involved in diseases due to harmful vascularization such as tumor growth or retinal neovascularization. Treating those blood vessels without harming healthy tissues is an issue. It requires specific and precise images of the blood vessels, both criteria being achievable thanks to fluorescent imaging. The specificity of fluorescent imaging relies on the use of a probe, meaning a selective fluorophore. To synthetize selective probes, we were inspired by a known ligand of the VEGFr: the axitinib. The chemical structure of the axitinib has an indazole heterocycle with two key roles: (i) in the fluorescence of the axitinib, (ii) in its selectivity for the VEGFr. Substituents were introduced to increase the overall fluorescence of the molecule while preserving the backbone responsible for the biological activity to the best of our ability. A library of about twenty fluorophores was synthetized and studied for applications in fluorescent imaging
APA, Harvard, Vancouver, ISO, and other styles
7

Goulas, Yves. "Teledetection de la fluorescence des couverts vegetaux : temps de vie de la fluorescence chlorophyllienne et fluorescence bleue." Paris 11, 1992. http://www.theses.fr/1992PA112264.

Full text
Abstract:
La mesure de la fluorescence de la chlorophylle in vivo est une methode d'investigation largement utilisee pour le suivi de l'activite photosynthetique, sur algues, chlorophastes ou feuilles entieres. Sur un couvert vegetal, la mesure a distance de la duree de vie de fluorescence permet de determiner directement le rendement quantique et peut ainsi etre envisagee comme methode de teledetection de l'etat physiologique de la vegetation. Dans cette perspective, nous avons etudie les possibilites qu'offre la fluorescence resolue en temps. Nous avons etudie l'effet du quenching energetique sur les composantes du declin de fluorescence et nous interpretons ces variations en termes de taille d'antenne et de limitation de la diffusion des excitons. Nous avons examine la faisabilite des mesures de duree de vie sur un couvert vegetal et defini les techniques instrumentales a mettre en uvre. Nous avons egalement developpe une methode specifique d'analyse qui permet de calculer la valeur de la duree de vie moyenne de fluorescence a partir des signaux emis par la canopee apres une impulsion lumineuse. A cote de l'emission rouge de la chlorophylle, les feuilles des vegetaux superieurs presentent une emission de fluorescence dans la region bleue du spectre apres excitation par un rayonnement ultraviolet. Cette emission a ete remarquee pour sa sensibilite au stress, mais son origine reste encore inconnue. Nous avons etabli les spectres d'excitation et d'emission des differents composes qui en sont responsables chez l'epinard, ce qui nous permet de proposer un certain nombre d'especes chimiques pour l'origine de cette emission. Nous avons etudie la faisabilite de sa detection a grande distance, simultanement avec l'emission chlorophyllienne, par la technique des lignes de fraunhofer et nous evaluons les performances attendues dans ce cas
APA, Harvard, Vancouver, ISO, and other styles
8

Cheng, Hok Yan. "Near infrared fluorescence probes : towards applications in fluorescence guided surgery." Thesis, University of Hull, 2017. http://hydra.hull.ac.uk/resources/hull:16529.

Full text
Abstract:
Surgery has been a popular method for the treatment of cancers, in particular solid tumours; but the surgical margins for cancerous tissues are often indistinct and in most cases, the poor identification of residual cancer tissues can result in re-excision. Therefore, near infrared (NIR) fluorescence-guided surgery (FGS) is being developed as a real time intra-operative imaging technique to assist surgeons by improving the accuracy and precision of the removal of tumours. However, current FDA approved fluorophores suffer from poor chemical stability, limited water-solubility, and lack selectivity toward neoplastic tissue, limiting their clinical application. These current challenges have led to the development of new and improved fluorophores capable of absorbing and emitting light at NIR wavelengths, negating autofluorescence and improving deeper light transmission. Throughout this project, a series of BODIPYs, aza-BODIPYs and bacteriochlorins were synthesised and developed for bioimaging applications. Despite many of them showing interesting fluorescence properties, the investigation suggested aza-BODIPYs were the most promising red / NIR fluorophores (λem 600-700 nm) due to their excellent photostability. Methods have been developed to incorporate functionalities suitable for bioconjugation. Different bioconjugation strategies have been explored to covalently conjugate the NIR fluorophores to a clinically relevant protein, peptide and antibody under mild conditions. The viability of aza-BODIPY conjugates against biological targets were investigated and a range of other novel targeted NIR fluorophores were successfully developed. In vitro fluorescence imaging was subsequently carried out to demonstrate the enhanced selectivity of the targeting NIR fluorophores toward overexpressed receptors on various cancer cells lines. This project has demonstrated the potential of aza-BODIPY in biological imaging and developed targeted NIR fluorophores. Further biological evaluation is progressing with the eventual aim of developing a pre-clinical model for NIR FGS in oncology.
APA, Harvard, Vancouver, ISO, and other styles
9

Zhou, Xiaobo. "Design, synthesis and sensing properties of chiral amine-based fluorescent probes." HKBU Institutional Repository, 2012. https://repository.hkbu.edu.hk/etd_ra/1442.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Aknine, Nathan. "Nouvelles sondes fluorescentes pour la bioimagerie des structures lipidiques cellulaires." Electronic Thesis or Diss., Strasbourg, 2024. http://www.theses.fr/2024STRAF035.

Full text
Abstract:
Ce projet de doctorat vise la conception de sondes fluorescentes innovantes, pour une meilleure visualisation et compréhension des structures lipidiques cellulaires par bioimagerie. Ainsi, trois familles de nouvelles sondes fluorescentes ont été générées dans le cadre de cette thèse, visant à cibler et éclairer différentes localisations cellulaires. Premièrement, le ciblage covalent de la membrane plasmique par une stratégie nommée MemGraft, a permis de développer de nouveaux outils pour le marquage et la fonctionnalisation de la surface cellulaire. Ces sondes permettent un marquage robuste et efficace, une imagerie long-terme, un suivi de plusieurs populations par étiquetage fluorescent multiplexé, mais également la manipulation du comportement des cellules, sans porter atteinte à leur phénotype par ingénierie de la surface cellulaire. Ensuite, des sondes sensibles à leur environnement, basées sur un groupement trifluoroacétyle a permis l’imagerie de la polarité et de l’hétérogénéité des gouttelettes lipidiques intracellulaires. Cette stratégie a également rendu possible l’imagerie en super-résolution de la polarité de membranes intracellulaires. Finalement, nous avons exploré des stratégies de ciblage des organites par des sondes solvatochromes, en particulier la mitochondrie, mais également le réticulum endoplasmique, l’appareil de Golgi, ou encore les lysosomes, fournissant des informations sur l’organisation lipidique de leur membrane. En combinant chimie organique, chimie biologique, et microscopies de fluorescence, ces nouveaux outils ont été conçus et démontrent leur potentiel pour améliorer notre compréhension des structures et des processus cellulaires<br>This PhD project is focused on the design of innovative fluorescent probes for better visualization and understanding of cellular lipidic structures by bioimaging. Three families of novel fluorescent probes have been generated in this thesis, targeting and shedding light on different cellular localizations. First, the lipiddirected covalent targeting of the plasma membrane was introduced and enabled the development of new fluorescent tools for the labeling and functionalization of the cell surface. These probes allowed robust longterm cell imaging and barcoding as well as cell manipulation and cell surface engineering. Secondly, environment-sensitive probes based on a trifluoroacetyl electron acceptor were obtained, enabling the imaging of the polarity and heterogeneity of intracellular lipid droplets. This strategy also yielded probes for super-resolution imaging of the polarity in intracellular membranes. Finally, we explored the strategy for targeting organelles with solvatochromic probes, in particular mitochondria, but also the endoplasmic reticulum, Golgi apparatus and lysosomes, providing insights on the lipid organization of their membranes through polarity imaging. Combining organic chemistry, chemical biology, and fluorescence microscopy, these new advanced molecular tools have been obtained and showed their potential to improve our understanding of cellular structures and processes
APA, Harvard, Vancouver, ISO, and other styles
More sources

Books on the topic "Fluorescence"

1

Slavík, Jan, ed. Fluorescence Microscopy and Fluorescent Probes. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

D, Slavík Jan Ph, and Conference on Fluorescence Microscopy and Fluorescent Probes (1995 : Prague, Czech Republic), eds. Fluorescence microscopy and fluorescent probes. New York: Plenum Press, 1996.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
3

Deatrick, Elizabeth. Fluorescence. [Chevy Chase, MD]: The author, 2017.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
4

B, Thompson Richard, ed. Fluorescence sensors and biosensors. Boca Raton, FL: Taylor&Francis, 2005.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
5

Kubitscheck, Ulrich, ed. Fluorescence Microscopy. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2017. http://dx.doi.org/10.1002/9783527687732.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Kubitscheck, Ulrich, ed. Fluorescence Microscopy. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2013. http://dx.doi.org/10.1002/9783527671595.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Wolfbeis, Otto S., ed. Fluorescence Spectroscopy. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-77372-3.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Valeur, Bernard, and Mário Nuno Berberan-Santos. Molecular Fluorescence. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2012. http://dx.doi.org/10.1002/9783527650002.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Herman, B. Fluorescence microscopy. 2nd ed. Singapore: BIOS, 1998.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
10

Dick, Jennifer K. Fluorescence: Poems. Athens: University of Georgia Press, 2004.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Book chapters on the topic "Fluorescence"

1

Gerritsen, Hans C. "Confocal Fluorescence Lifetime Imaging." In Fluorescence Microscopy and Fluorescent Probes, 35–46. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_3.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Cimprich, Petr, and Jan Slavík. "Artifacts in Fluorescence Ratio Imaging." In Fluorescence Microscopy and Fluorescent Probes, 119–23. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_16.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

O’Connor, José-Enrique. "Flow Cytometry versus Fluorescence Microscopy." In Fluorescence Microscopy and Fluorescent Probes, 61–66. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_6.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Gundlach, Heinz. "Multichannel Fluorescence Microscopy and Digital Imaging - On the Exciting Developments in Fluorescence Microscopy." In Fluorescence Microscopy and Fluorescent Probes, 67–70. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_7.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Herman, Brian. "Fluorescence Microscopy: State of the Art." In Fluorescence Microscopy and Fluorescent Probes, 1–14. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_1.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Tengholm, Anders, and Eva Grapengiesser. "Disappearance of Cytoplasmic Ca2+ Oscillations is a Sensitive Indicator of Photodamage in Pancreatic β-Cells." In Fluorescence Microscopy and Fluorescent Probes, 85–89. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_10.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Cimprich, Petr, and Jan Slavík. "Distribution of Individual Cytoplasmic pH Values in a Cell Suspension." In Fluorescence Microscopy and Fluorescent Probes, 91–93. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_11.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Gregor, Martin, Jan Tachezy, and Jan Slavík. "The Effect of Lysosomal pH on Lactoferrin-Dependent Iron Uptake in Tritrichomonas foetus." In Fluorescence Microscopy and Fluorescent Probes, 95–99. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_12.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Opitz, N., T. Porwol, E. Merten, and H. Acker. "On the Protein-Error of the Calcium-Sensitive Fluorescent Indicator Fura-Red." In Fluorescence Microscopy and Fluorescent Probes, 101–6. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_13.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Opitz, N., T. Porwol, E. Merten, and H. Acker. "Cytoplasmic Ion Imaging: Evidence for Intracellular Calibration Heterogeneities of Ion-Sensitive Fluoroprobes." In Fluorescence Microscopy and Fluorescent Probes, 107–12. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_14.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "Fluorescence"

1

Johnson, Richard E., and Vinod S. Agarwala. "Fluorescence Based Chemical Sensors for Corrosion Detection." In CORROSION 1997, 1–7. NACE International, 1997. https://doi.org/10.5006/c1997-97304.

Full text
Abstract:
Abstract Several fluorescent materials have been identified as possible corrosion sensing coatings. These are either redox or metal ion complex materials. The redox materials are nonfluorescent in the reduced state and become fluorescent upon oxidation. Incorporated into paint coatings, they provide an early warning of corrosive conditions at the metal or alloy surface. The metal ion complex materials only fluoresce when the organic compound complexes with metal ions such as those generated in corrosion reactions. Fluorescent materials have been incorporated into paint coatings and on metal surfaces for the detection of corrosion. Oxine reacts with aluminum oxide on corroded aluminum to give a fluorescence that can be photographed in UV light. Several other materials were found to have good fluorescence but cannot be reversibly oxidized or reduced at the present time. More work will be done with these compounds as well as with Schiff bases to develop new fluorescent chemical sensing materials for smart coating on alloy surfaces.
APA, Harvard, Vancouver, ISO, and other styles
2

Abbey, Emma, Oren Katz, Travis Ferguson, Sarah-Johanna Klose, Chris Pruefert, and Hans-Peter Loock. "Applications of Hadamard-Multiplexed Fluorescence Imaging using a Hyperspectral Camera." In CLEO: Applications and Technology, AM4A.2. Washington, D.C.: Optica Publishing Group, 2024. http://dx.doi.org/10.1364/cleo_at.2024.am4a.2.

Full text
Abstract:
We present a hyperspectral fluorescence imaging system based on a Hadamard-multiplexed light source for monitoring spatially inhomogeneous reactions through time and correctly identifying fluorescent components in complex mixtures.
APA, Harvard, Vancouver, ISO, and other styles
3

Bradley, Mark. "Fluorescence and fluorescent life imaging probes for biomedical applications." In Reporters, Contrast Agents, and Molecular Probes for Biomedical Applications XVI, edited by Ramesh Raghavachari and Mikhail Y. Berezin, 1. SPIE, 2025. https://doi.org/10.1117/12.3049934.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Durrett, Michael G., Richard E. Johnson, and Vinod S. Agarwala. "ICI, Intelligent Corrosion Indicator, and Its Use for the Early Detection of Corrosion on Aluminum Alloy Surfaces by Fluorescence." In CORROSION 2000, 1–8. NACE International, 2000. https://doi.org/10.5006/c2000-00285.

Full text
Abstract:
Abstract Several fluorescent materials have been used under epoxy coatings on 7075 aluminum alloy surfaces. These fluorescent materials either fluoresce upon oxidation and are thus corrosivity indicators or they fluoresce with the products of corrosion and are true corrosion indicators. Recent work has involved using a pigmented epoxy paint, such as that used on aircraft, to see if fluorescence is still visible. Although the intensity is reduced, there is still visible fluorescence. This paper reports the results of the most promising fluorescent markers in each of the two types mentioned above. Photographic data are presented with changes of fluorescent activity with time. These two types of indicators have the potential to detect corrosion or corrosive conditions under paint coatings and could greatly reduce the costs of corrosion maintenance as well as enhancing safety factors in aircraft.
APA, Harvard, Vancouver, ISO, and other styles
5

Schreiter, Eric. "Chemigenetic Fluorescent Biosensors." In Optics and the Brain, BS1C.2. Washington, D.C.: Optica Publishing Group, 2024. https://doi.org/10.1364/brain.2024.bs1c.2.

Full text
Abstract:
We have developed chemigenetic fluorescent indicators built from engineered protein sensor domains and bright fluorescent dyes. Advantages include increased photon output for demanding microscopy applications and bright fluorescence at wavelengths not well covered by fluorescent proteins. Full-text article not available; see video presentation
APA, Harvard, Vancouver, ISO, and other styles
6

Panova, Anna A., Paul Pantano, and David R. Walt. "In Situ Fluorescence Imaging of Localized Corrosion with a pH-Sensitive Imaging Fiber." In CORROSION 1997, 1–4. NACE International, 1997. https://doi.org/10.5006/c1997-97314.

Full text
Abstract:
Abstract A fiber optic pH-sensor capable of both visualizing corrosion sites and measuring local chemical concentrations is applied to real-time corrosion monitoring. The imaging fiber’s distal face containing an immobilized pH-sensitive fluorescent dye is brought into contact with metal surfaces submerged in aqueous buffers and fluorescence images are acquired as a function of time. The observed changes in fluorescence due to increases in pH at cathodic sites and decreases in pH at anodic sites are indicative of localized corrosion rates.
APA, Harvard, Vancouver, ISO, and other styles
7

Armstrong, R. L., J. G. Xie, T. E. Ruskgauer, and R. G. Pinnick. "Energy transfer lasing from dye-doped microdroplets seeded with fluorescent sol." In OSA Annual Meeting. Washington, D.C.: Optica Publishing Group, 1992. http://dx.doi.org/10.1364/oam.1992.tug2.

Full text
Abstract:
We report observations of lasing and fluorescence emission from liquid microdroplets of fluorescein 548 dye in ethanol, seeded with submicrometer sized fluorescent sol. An incident pump laser generates droplet fluo­rescein lasing or fluorescene, which in turn excites either fluorescene or lasing in the sol. All emissions are at wavelengths corresponding to morphology dependent reso­nances (MDR's) of the droplet. Studies of the dependence of these emissions on pump laser intensity and sol concentration suggest they are driven by enhanced radiative energy transfer that occurs when fluorescein lasing or fluorescence emission couples to MDR's of the droplet microcavity. Other noteworthy findings include the absence of sol emission for larger sol and the presence of sol emission, even without any observable fluorescein emission, for smaller sol.
APA, Harvard, Vancouver, ISO, and other styles
8

Maruoka, Shoji, Yohei Mitsui, Shinpei Okawa, Yoko Hoshi, and Yukio Yamada. "Measurement of Fluorescence Properties in Light Scattering Medium." In ASME/JSME 2011 8th Thermal Engineering Joint Conference. ASMEDC, 2011. http://dx.doi.org/10.1115/ajtec2011-44458.

Full text
Abstract:
The purpose of this study is to measure the fluorescence properties of Indocyaninegreen (ICG) which is a fluorescence dye to be used as a fluorescence probe for the use of fluorescence imaging in biomedical applications. The fluorescence molecular imaging is expected to solve the issues in preclinical studies which require a lot of time, labors and sacrificed animals. Information of living body can be obtained by measuring the fluorescent properties of the probe in biological media. The absorption and emission spectra and the lifetime of ICG in non-scattering and scattering media were measured in this study. ICG was dissolved in water, in plasma, in Intralipid, and in a mixture of plasma and Intralipid to simulate the environment in living tissues. The absorption and emission spectra were measured using a fluorescence spectrophotometer. The fluorescence lifetimes were measured using a time-resolved measurement method. Results suggest that the fluorescent properties are affected by the reaction between ICG and biological tissues.
APA, Harvard, Vancouver, ISO, and other styles
9

Deliolanis, Nikolaos C., Thomas Wurdinger, Bakhos Tannous, and Vasilis Ntziachristos. "Fluorescence Tomography of Red-shifted Fluorescent Proteins." In Biomedical Optics. Washington, D.C.: OSA, 2010. http://dx.doi.org/10.1364/biomed.2010.btud4.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Kawata, Satoshi, and Rieko Arimoto. "Laser-scan fluorescence microscope with annular excitation optics." In OSA Annual Meeting. Washington, D.C.: Optica Publishing Group, 1990. http://dx.doi.org/10.1364/oam.1990.mpp1.

Full text
Abstract:
We discuss the three-dimensional imaging characteristics of a laser-scan fluorescence microscope with an annular pupil in the excitation optics. As is well known, the use of an annular pupil in a conventional incoherent imaging system increases the depth of focus and the lateral resolution as compared with the use of a circular pupil.1,2 However, an annular pupil has not been practically used for fluorescence microscopy because it stops a large amount of the fluorescent light arriving at the objective lens. In the case of a laser-scan fluorescence microscope, we may place an annular pupil in the excitation optics rather than in the imaging optics. In this case, we do not waste fluorescent light because the objective-lens pupil is fully open.
APA, Harvard, Vancouver, ISO, and other styles

Reports on the topic "Fluorescence"

1

Hargis, P. J. Jr, B. L. Preppernau, and B. P. Aragon. Ultraviolet fluorescence monitor. Office of Scientific and Technical Information (OSTI), May 1997. http://dx.doi.org/10.2172/481480.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Allison, S. W. Fluorescence Rise Time Measurements for High Temperature Fluorescence-Based Thermometry. Office of Scientific and Technical Information (OSTI), March 2005. http://dx.doi.org/10.2172/885970.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

DeRose, Paul C. Standard guide to fluorescence :. Gaithersburg, MD: National Institute of Standards and Technology, 2007. http://dx.doi.org/10.6028/nist.ir.7458.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Haugland, R. P. Fluorescence-detected DNA sequencing. Office of Scientific and Technical Information (OSTI), January 1990. http://dx.doi.org/10.2172/5619036.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Deng, Chun, Zhenyu Zhang, Zhi Guo, Hengduo Qi, Yang Liu, Haimin Xiao, and Xiaojun Li. Assessment of intraoperative use of indocyanine green fluorescence imaging on the number of lymph node dissection during minimally invasive gastrectomy: a systematic review and meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, November 2021. http://dx.doi.org/10.37766/inplasy2021.11.0062.

Full text
Abstract:
Review question / Objective: Whether is indocyanine green fluorescence imaging-guided lymphadenectomy feasible to improve the number of lymph node dissections during radical gastrectomy in patients with gastric cancer undergoing curative resection? Condition being studied: Gastric cancer was the sixth most common malignant tumor and the fourth leading cause of cancer-related death in the world. Radical lymphadenectomy was a standard procedure in radical gastrectomy for gastric cancer. The retrieval of more lymph nodes was beneficial for improving the accuracy of tumor staging and the long-term survival of patients with gastric cancer. Indocyanine green(ICG) near-infrared fluorescent imaging has been found to provide surgeons with effective visualization of the lymphatic anatomy. As a new surgical navigation technique, ICG near-infrared fluorescent imaging was a hot spot and had already demonstrated promising results in the localization of lymph nodes during surgery in patients with breast cancer, non–small cell lung cancer, and gastric cancer. In addition, ICG had increasingly been reported in the localization of tumor, lymph node dissection, and the evaluation of anastomotic blood supply during radical gastrectomy for gastric cancer. However, it remained unclear whether ICG fluorescence imaging would assist surgeons in performing safe and sufficient lymphadenectomy.
APA, Harvard, Vancouver, ISO, and other styles
6

Mathies, R. A., and A. N. Glazer. Ultrasensitive fluorescence detection of DNA. Office of Scientific and Technical Information (OSTI), January 1992. http://dx.doi.org/10.2172/7176600.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Mathies, R. A., and A. N. Glazer. Ultrasensitive fluorescence detection of DNA. Office of Scientific and Technical Information (OSTI), January 1991. http://dx.doi.org/10.2172/5827344.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Guo, Yixing. Fluorescence Detection of Biological Thiols. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.586.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

ภุมมางกูร, สุนิพนธ์, ราณี สุรกาญจน์กุล та ลาวัลย์ ศรีพงษ์. การตรวจสอบเภสัชภัณฑ์โดยใช้ฟลูโอเรสคามีน : รายงานผลการวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 1989. https://doi.org/10.58837/chula.res.1989.10.

Full text
Abstract:
การใช้ fluorescamine เป็น spraying reagent พ่นบน plate ที่เคลือบด้วย silica gel GF 254 สามารถใช้ตรวจสอบยาที่มีหมู่ฟังก์ชัน –NH2, -NH, และ –N- ได้ พบว่ายาที่เป็น primary amine จะเกิด fluorescence กับ fluorescamine แต่ยาที่เป็น secondary amine จะเกิด quenching ส่วนที่เป็น tertiary amine หรือ quarternary amine จะไม่เกิดอะไรกับ fluorescamine โดยอาศัยผลการทดลองอันนี้จะทำให้สามารถแบ่งยาเป็น 3 กลุ่ม คือกลุ่มที่ 1 เป็นยาที่เกิด fluorescence กับ fluorescamine กลุ่มที่ 2 เกิด quenching และกลุ่มที่ 3 ไม่เกิด fluorescence หรือ quenching กับ fluorescamine โดยอาศัยการแบ่งยาเป็นสามกลุ่มและการใช้ข้อมูลจาก Rf value ใน 3 solvent systems การใช้ไอของไอโอดีน ทำให้สามารถตรวจสอบหรือพิสูจน์เอกลักษณ์ของยาได้
APA, Harvard, Vancouver, ISO, and other styles
10

Scalettar, Bethe A. Fluorescence spectroscopic studies of DNA dynamics. Office of Scientific and Technical Information (OSTI), April 1987. http://dx.doi.org/10.2172/10150990.

Full text
APA, Harvard, Vancouver, ISO, and other styles
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!