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1

Kaur, Harpreet, Khanh Nguyen, and Pradeep Kumar. "Pressure and temperature dependence of fluorescence anisotropy of green fluorescent protein." RSC Advances 12, no. 14 (2022): 8647–55. http://dx.doi.org/10.1039/d1ra08977c.

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2

Grzywacz, Jerzy, and Zygmunt Trumpakaj. "Influence of Inertial Effect on Fluorescence Anisotropy." Zeitschrift für Naturforschung A 42, no. 2 (1987): 123–26. http://dx.doi.org/10.1515/zna-1987-0203.

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The influence of inertial effects on the fluorescence anisotropy r is discussed.From recent work on the anisotropy of a prolate fluorescent molecule in a liquid solvent it is known that its estimated experimental moment of inertia is as much as about 3 orders of magnitude greater than that calculated from its geometry.In this paper, by using a non-exponential form of the memory function K (t) in the generalized relaxation equation for r (t), a satisfactory agreement between measured and calculated moments of inertia is obtained.
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3

Czajkowsky, Daniel M. "Fluorescence anisotropy of oligomeric proteins." Spectroscopy 18, no. 1 (2004): 85–93. http://dx.doi.org/10.1155/2004/460353.

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Previous studies of protein oligomerization using time-resolved fluorescence anisotropy assumed a single fixed probe per oligomeric complex and an identical probe orientation in complexes of different stoichiometry. However, an oligomer consisting of “n” singly labeled monomers must necessarily have “n” probes. Moreover, in the expression for the anisotropy decay, the molecular axes from which the probe orientation is defined are different for complexes that differ in stoichiometry. Here, we derive an expression for the decay of the anisotropy for molecules with any number of fixed probes, and
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4

Kawski, A., I. Gryczyński, and Z. Gryczyński. "Fluorescence and Phosphorescence Anisotropy Spectra of Indole in Poly (Vinyl Alcohol) Film at Room Temperature." Zeitschrift für Naturforschung A 49, no. 11 (1994): 1091–92. http://dx.doi.org/10.1515/zna-1994-1118.

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Abstract Excitation anisotropy spectra of indole were measured in poly (vinyl alcohol) film at room temperature. The behaviour of the phosphorescence anisotropy of indole in isotropic and anisotropic PVA film enabled a conclusion to be drawn that the T1 - S0 transition is located outside the indole ring plane, close to the perpendicular direction.
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5

Zhou, Zhenpeng, Xin Yan, Yin-Hung Lai, and Richard N. Zare. "Fluorescence Polarization Anisotropy in Microdroplets." Journal of Physical Chemistry Letters 9, no. 11 (2018): 2928–32. http://dx.doi.org/10.1021/acs.jpclett.8b01129.

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6

Levitt, James A., Pei‐Hua Chung, Marina K. Kuimova, et al. "Fluorescence Anisotropy of Molecular Rotors." ChemPhysChem 12, no. 3 (2011): 662–72. http://dx.doi.org/10.1002/cphc.201000782.

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7

Soleimaninejad, Hamid, Kenneth P. Ghiggino, Trevor A. Smith, and Matthew F. Paige. "Fluorescence anisotropy imaging of a polydiacetylene photopolymer film." Canadian Journal of Chemistry 97, no. 6 (2019): 422–29. http://dx.doi.org/10.1139/cjc-2018-0360.

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UV-illumination of phase-separated surfactant films prepared from mixtures of photopolymerizable 10,12-pentacosadiynoic acid and perfluorotetradecanoic acid results in the formation of fluorescent polydiacetylene fibers and aggregates. In this work, the orientation of polymer strands that comprise the resulting photopolymer structures has been probed using fluorescence anisotropy imaging in combination with defocused single-molecule fluorescence imaging. Imaging experiments indicate the presence of significant fiber-to-fiber heterogeneity, as well as anisotropy within each fiber (or aggregate)
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8

Gorbunova, Ioanna A., Maxim E. Sasin, Anna A. Zhikhoreva, et al. "Fluorescence Anisotropy in Radachlorin and Chlorin e6 in Water–Methanol Solutions under One- and Two-Photon Excitation." Photonics 10, no. 1 (2022): 9. http://dx.doi.org/10.3390/photonics10010009.

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The fluorescence anisotropy of photosensitizers Radachlorin and chlorin e6 was studied using the time-resolved single photon-counting technique under one- and two-photon excitation within the Soret absorption band. A very small negative anisotropy was observed in both photosensitizers under one-photon excitation in the vicinity of the absorption maximum within the wavelength range of 395–405 nm. Meanwhile, two-photon excitation of the photosensitizers in the same spectral range demonstrated high fluorescence anisotropy with the maximum value of about 0.43. The drastic difference of the fluores
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9

Viovy, Jean Louis. "Anisotropic rotation of 1,9-dimethylanthracene: a fluorescence anisotropy decay study." Journal of Physical Chemistry 89, no. 25 (1985): 5465–72. http://dx.doi.org/10.1021/j100271a030.

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10

Jain, Puneet, Takuya Aida, and Masahiro Motosuke. "Fluorescence Anisotropy as a Temperature-Sensing Molecular Probe Using Fluorescein." Micromachines 12, no. 9 (2021): 1109. http://dx.doi.org/10.3390/mi12091109.

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Fluorescence anisotropy, a technique to study the folding state of proteins or affinity of ligands, is used in this present work as a temperature sensor, to measure the microfluidic temperature field, by adding fluorophore in the liquid. Fluorescein was used as a temperature-sensing probe, while glycerol–aq. ammonia solution was used as a working fluid. Fluorescence anisotropy of fluorescein was measured by varying various parameters. Apart from this, a comparison of fluorescence anisotropy and fluorescence intensity is also performed to demonstrate the validity of anisotropy to be applied in
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11

Grisanti, Luca, Francesca Terenziani, Cristina Sissa, et al. "Polar Fluorenes and Spirobifluorenes: Fluorescence and Fluorescence Anisotropy Spectra." Journal of Physical Chemistry B 115, no. 39 (2011): 11420–30. http://dx.doi.org/10.1021/jp206592s.

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12

Semenov, Alexey N., Daniil A. Gvozdev, Anastasia M. Moysenovich, et al. "Probing Red Blood Cell Membrane Microviscosity Using Fluorescence Anisotropy Decay Curves of the Lipophilic Dye PKH26." International Journal of Molecular Sciences 23, no. 24 (2022): 15767. http://dx.doi.org/10.3390/ijms232415767.

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Red blood cell (RBC) aggregation and deformation are governed by the molecular processes occurring on the membrane. Since several social important diseases are accompanied by alterations in RBC aggregation and deformability, it is important to develop a diagnostic parameter of RBC membrane structural integrity and stability. In this work, we propose membrane microviscosity assessed by time-resolved fluorescence anisotropy of the lipophilic PKH26 fluorescent probe as a diagnostic parameter. We measured the fluorescence decay curves of the PKH26 probe in the RBC membrane to establish the optimal
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13

Ameloot, Marcel, Martin vandeVen, A. Ulises Acuña, and Bernard Valeur. "Fluorescence anisotropy measurements in solution: Methods and reference materials (IUPAC Technical Report)." Pure and Applied Chemistry 85, no. 3 (2013): 589–608. http://dx.doi.org/10.1351/pac-rep-11-11-12.

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After recalling the basic relations relevant to both steady-state and time-resolved fluorescence polarization, it is shown how the values of steady-state polarized intensities recorded experimentally usually need to be corrected for systematic effects and errors, caused by instrumentation and sample properties. A list of selected reference values of steady-state fluorescence anisotropy and polarization is given. Attention is also paid to analysis of time-resolved fluorescence anisotropy data obtained by pulse fluorometry or phase and modulation fluorometry techniques. Recommendations for check
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14

Wölcke, Julian, Nicholas Hunt, Joern Jungmann, and Dirk Ullmann. "Early Identification of False Positives in High-Throughput Screening for Activators of p53-DNA Interaction." Journal of Biomolecular Screening 11, no. 4 (2006): 341–50. http://dx.doi.org/10.1177/1087057106286652.

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Naturally occurring mutant forms of p53 are deficient for specific DNA binding. However, their specific DNA binding can be reactivated. The search for small molecules that reactivate latent p53 is considered to be a cornerstone in cancer therapy. The authors describe a new homogeneous fluorescent assay approach for the characterization of binding affinities of human wild-type latent and activated p53 using DNA*spec(26), with and without the addition of the antibody PAb421, respectively, and fluorescence correlation spectroscopy (FCS)/2-dimensional fluorescence-intensity distribution analysis a
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15

Paloncýová, Markéta, Marcel Ameloot, and Stefan Knippenberg. "Orientational distribution of DPH in lipid membranes: a comparison of molecular dynamics calculations and experimental time-resolved anisotropy experiments." Physical Chemistry Chemical Physics 21, no. 14 (2019): 7594–604. http://dx.doi.org/10.1039/c8cp07754a.

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The behavior of the fluorescent probe diphenylhexatriene (DPH) in different lipid phases is investigated. The rotational autocorrelation functions are calculated in order to model the time-resolved fluorescence anisotropy decay. The role of the order parameters is discussed.
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16

Gryczynski, Ignacy, Zygmunt Gryczynski, and Joseph R. Lakowicz. "Fluorescence Anisotropy Controlled by Light Quenching." Photochemistry and Photobiology 67, no. 6 (1998): 641–46. http://dx.doi.org/10.1111/j.1751-1097.1998.tb09467.x.

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17

Pusey, M. L. "Fluorescence anisotropy-based macromolecule crystallization screening." Acta Crystallographica Section A Foundations of Crystallography 67, a1 (2011): C766. http://dx.doi.org/10.1107/s0108767311080640.

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18

Christensen, Ronald L., Rodney C. Drake, and David Phillips. "Time-resolved fluorescence anisotropy of perylene." Journal of Physical Chemistry 90, no. 22 (1986): 5960–67. http://dx.doi.org/10.1021/j100280a100.

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19

Bigger, Stephen W., Robert A. Craig, Kenneth P. Ghiggino, and John Scheirs. "Fluorescence anisotropy measurements in undergraduate teaching." Journal of Chemical Education 70, no. 9 (1993): A234. http://dx.doi.org/10.1021/ed070pa234.

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20

Schrell, Adrian M., Nikita Mukhitov, and Michael G. Roper. "Multiplexing Fluorescence Anisotropy Using Frequency Encoding." Analytical Chemistry 88, no. 16 (2016): 7910–15. http://dx.doi.org/10.1021/acs.analchem.6b02131.

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21

Lippitsch, Max E. "Optical sensors based on fluorescence anisotropy." Sensors and Actuators B: Chemical 11, no. 1-3 (1993): 499–502. http://dx.doi.org/10.1016/0925-4005(93)85293-j.

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22

Vinegoni, Claudio, Paolo Fumene Feruglio, Ignacy Gryczynski, Ralph Mazitschek, and Ralph Weissleder. "Fluorescence anisotropy imaging in drug discovery." Advanced Drug Delivery Reviews 151-152 (November 2019): 262–88. http://dx.doi.org/10.1016/j.addr.2018.01.019.

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23

Ingersoll, Christine M., A. Neal Watkins, Gary A. Baker, and Frank V. Bright. "Tracking Nanosecond and Subnanosecond Protein Dynamics On-the-Fly Using Frequency-Domain Fluorescence." Applied Spectroscopy 52, no. 7 (1998): 933–42. http://dx.doi.org/10.1366/0003702981944742.

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Fluorescence anisotropy and intensity decay experiments on proteins can provide detailed information on biomolecule dynamics and function. However, experiments of this sort are normally performed while the biomolecule is at or near equilibrium. Although information on protein dynamics under equilibrium conditions is extremely important, details about the protein behavior while it is actually undergoing change can provide significantly more insight into the overall protein behavior. Multiharmonic Fourier frequency-domain fluorescence provides a means to acquire fluorescence anisotropy and inten
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24

Tong, Zhi Peng, Shou Zhi Pu, and Shi Qiang Cui. "Photoinduced Anisotropy in a New Diarylethene Bearing Five and Six-Membered Aryl." Advanced Materials Research 490-495 (March 2012): 3387–90. http://dx.doi.org/10.4028/www.scientific.net/amr.490-495.3387.

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A new symmetrical photochromic diarylethene, 1-(2-methyl-5-formyl)-2-(2- cyanophenyl) perfluorocyclopentene(1a), was synthesized, and its photochromic properties were investigated in detail. The compound exhibited good photochromism both in solution and in PMMA film with alternating irradiation by UV/VIS light, and the maxima absorption of its closed-ring isomer 1b are 544 and 549 nm, respectively. The photochromism properties of 1a diarylethene show good photochromism in hexane and in PMMA amorphous film. Steady state fluorescence studies indicated that 1a diarylethene is higher fluorescent i
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25

Naumenko, A. P., V. I. Borysuk, M. S. Iakhnenko, V. O. Gubanov, Y. L. Slominskii, and O. D. Kachkovsky. "Probing of Higher Excited States of Merocyanine Derivatives of Azaazulene and Indandione by Fluorescence Excitation Anisotropy Spectra." Ukrainian Journal of Physics 65, no. 4 (2020): 321. http://dx.doi.org/10.15407/ujpe65.4.321.

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This paper is dedicated to the spectral and quantum-chemical studies of higher excited states of merocyanine derivatives of azaazulene and indandione. A particular attention is paid to the analysis of fluorescence excitation anisotropy spectra of the mentioned compounds. The long-wave shift by ≈ 50 nm of a deep clear minimum in the fluorescence excitation anisotropy spectrum due to an elongation of the polymethine dye chromophore is established. Such shift is close in a value to the bathochromic shift of the first minimum in the anisotropy spectrum of symmetric ionic polymethine dyes, in which
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26

Dudkowiak, Alina, Danuta Frackowiak, Ewa Teślak, Zygmunt Gryczyński, and Ignacy Gryczyński. "Orientation and spectral properties of two stilbazolium merocyanine dyes in stretched and unstretched polyvinyl alcohol films." Acta Biochimica Polonica 54, no. 3 (2007): 647–56. http://dx.doi.org/10.18388/abp.2007_3238.

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Spectral properties (anisotropy coefficients calculated for absorption, emission and fluorescence decay time) of two stilbazolium merocyanine dyes have been determined to evaluate the applicability of these dyes as sensitizers in photodynamic therapy. The dyes were embedded in an anisotropic polymer matrix. Analysis of the emission decay components measured in polarized light provides information on the interactions of the dye molecules with the polymer matrix being a model of an anisotropic biological system. Different values of the emission anisotropies obtained from various polarized compon
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27

Xiao, Xue, and Shujun Zhen. "Recent advances in fluorescence anisotropy/polarization signal amplification." RSC Advances 12, no. 11 (2022): 6364–76. http://dx.doi.org/10.1039/d2ra00058j.

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We discuss how the potential of fluorescence anisotropy/polarization signal approach expanded through mass amplification, fluorescence lifetime amplification, segmental motion amplification, and provide perspectives at future applications.
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28

Lakowicz, Joseph R., Henryk Cherek, Józef Kuśba, Ignacy Gryczynski, and Michael L. Johnson. "Review of fluorescence anisotropy decay analysis by frequency-domain fluorescence spectroscopy." Journal of Fluorescence 3, no. 2 (1993): 103–16. http://dx.doi.org/10.1007/bf00865324.

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29

Sen, Sobhan, Dipankar Sukul, Partha Dutta, and Kankan Bhattacharyya. "Fluorescence Anisotropy Decay in Polymer−Surfactant Aggregates." Journal of Physical Chemistry A 105, no. 32 (2001): 7495–500. http://dx.doi.org/10.1021/jp004275c.

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30

Bigelow, Chad E., David L. Conover, and Thomas H. Foster. "Confocal fluorescence spectroscopy and anisotropy imaging system." Optics Letters 28, no. 9 (2003): 695. http://dx.doi.org/10.1364/ol.28.000695.

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31

Crane, Bryan L., N. Catherine Hogan, Hiroko Sudo, William G. Thilly, and Ian W. Hunter. "Real-Time PCR Measurement by Fluorescence Anisotropy." Analytical Chemistry 77, no. 16 (2005): 5129–34. http://dx.doi.org/10.1021/ac050323j.

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32

Mejillano, Magdalena R., and Richard H. Himes. "Tubulin dimer dissociation detected by fluorescence anisotropy." Biochemistry 28, no. 15 (1989): 6518–24. http://dx.doi.org/10.1021/bi00441a053.

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33

Piston, D. W., and E. Gratton. "Orientational exchange approach to fluorescence anisotropy decay." Biophysical Journal 56, no. 6 (1989): 1083–91. http://dx.doi.org/10.1016/s0006-3495(89)82756-0.

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34

Kimaru, Irene W., Yafei Xu, and Matthew E. McCarroll. "Characterization of Chiral Interactions Using Fluorescence Anisotropy." Analytical Chemistry 78, no. 24 (2006): 8485–90. http://dx.doi.org/10.1021/ac061335n.

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35

Jameson, David M., and Justin A. Ross. "Fluorescence Polarization/Anisotropy in Diagnostics and Imaging." Chemical Reviews 110, no. 5 (2010): 2685–708. http://dx.doi.org/10.1021/cr900267p.

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36

SANZVICENTE, I., J. CASTILLO, and J. GALBAN. "Fluorescence anisotropy: application in quantitative enzymatic determinations." Talanta 65, no. 4 (2005): 946–53. http://dx.doi.org/10.1016/j.talanta.2004.08.022.

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37

Mocz, Gabor. "Information Content of Fluorescence Polarization and Anisotropy." Journal of Fluorescence 16, no. 4 (2006): 511–24. http://dx.doi.org/10.1007/s10895-006-0095-7.

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38

Wang, Lin, Brendan Clifford, Lacey Graybeal, Luke Tolley, and Matthew E. McCarroll. "Detection of Target Proteins by Fluorescence Anisotropy." Journal of Fluorescence 23, no. 5 (2013): 881–88. http://dx.doi.org/10.1007/s10895-013-1194-x.

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39

Barbero, Nadia, Lucia Napione, Pierluigi Quagliotto, et al. "Fluorescence anisotropy analysis of protein–antibody interaction." Dyes and Pigments 83, no. 2 (2009): 225–29. http://dx.doi.org/10.1016/j.dyepig.2009.04.011.

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40

Burghardt, Thomas P., James E. Lyke, and Katalin Ajtai. "Fluorescence emission and anisotropy from rhodamine dimers." Biophysical Chemistry 59, no. 1-2 (1996): 119–31. http://dx.doi.org/10.1016/0301-4622(95)00118-2.

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41

MATTHEWS, D. R., L. M. CARLIN, E. OFO, et al. "Time-lapse FRET microscopy using fluorescence anisotropy." Journal of Microscopy 237, no. 1 (2010): 51–62. http://dx.doi.org/10.1111/j.1365-2818.2009.03301.x.

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42

Xu, Yafei, and Matthew E. McCarroll. "Determination of Enantiomeric Composition by Fluorescence Anisotropy." Journal of Physical Chemistry A 108, no. 34 (2004): 6929–32. http://dx.doi.org/10.1021/jp0472414.

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43

Burrows, Sean M., and Dimitri Pappas. "Measuring complexation by single-molecule fluorescence anisotropy." Analyst 133, no. 7 (2008): 870. http://dx.doi.org/10.1039/b800110c.

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44

Chen, Yin-Chu, Zheming Wang, Mingdi Yan, and Scott A. Prahl. "Fluorescence anisotropy studies of molecularly imprinted polymers." Luminescence 21, no. 1 (2006): 7–14. http://dx.doi.org/10.1002/bio.874.

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45

Jullien, Magali, and Marie-Pierre Crosio. "Study of Ribonuclease precrystallization by fluorescence anisotropy." Journal of Crystal Growth 110, no. 1-2 (1991): 182–87. http://dx.doi.org/10.1016/0022-0248(91)90883-7.

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46

Reiter, Michal, Pavel Heřman, and Ivan Barvík. "The anisotropy of fluorescence in ring units." Journal of Luminescence 110, no. 4 (2004): 258–63. http://dx.doi.org/10.1016/j.jlumin.2004.08.018.

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47

Myslinski, Piotr, Dariusz Wieczorek, and Krzysztof Kownacki. "Picosecond fluorescence anisotropy measured by frequency conversion." Chemical Physics Letters 155, no. 3 (1989): 256–61. http://dx.doi.org/10.1016/0009-2614(89)85320-5.

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48

Devauges, Viviane, Simon P. Poland, James Monypenny, Anthony H. Keeble, Andrew J. Beavil, and Simon M. Ameer-Beg. "Towards Single Molecule Imaging of Fluorescence Anisotropy." Biophysical Journal 110, no. 3 (2016): 175a. http://dx.doi.org/10.1016/j.bpj.2015.11.976.

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49

Bashmakova, Nataliia V., Yevgeniy O. Shaydyuk, Andriy M. Dmytruk, et al. "Nature of Linear Spectral Properties and Fast Electronic Relaxations in Green Fluorescent Pyrrolo[3,4-c]Pyridine Derivative." International Journal of Molecular Sciences 22, no. 11 (2021): 5592. http://dx.doi.org/10.3390/ijms22115592.

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The electronic nature of 4-hydroxy-1H-pyrrolo[3,4-c]pyridine-1,3,6(2H,5H)-trione (HPPT) was comprehensively investigated in liquid media at room temperature using steady-state and time-resolved femtosecond transient absorption spectroscopic techniques. The analysis of the linear photophysical and photochemical parameters of HPPT, including steady-state absorption, fluorescence and excitation anisotropy spectra, along with the lifetimes of fluorescence emission and photodecomposition quantum yields, revealed the nature of its large Stokes shift, specific changes in the permanent dipole moments
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50

Jakuš, Vladimír, Uwe Fuhr, Wolfgang Wörner, and Norbert Rietbrock. "Erythrocyte Membrane Fluidity in Diabetics: Fluorescence Study." Collection of Czechoslovak Chemical Communications 64, no. 3 (1999): 548–52. http://dx.doi.org/10.1135/cccc19990548.

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Erythrocyte membrane fluidity is changed in diabetic subjects with long-term complications. As membrane fluidity indicator, the mean steady-state fluorescence anisotropy was measured in 1,6-diphenylhexa-1,3,5-triene labelled erythrocyte membranes prepared from six control healthy donors and six poorly controlled diabetic subjects. Fluorescence anisotropy values of membranes prepared from erythrocytes of diabetic subjects were significantly higher than in control subjects. This indicates a decreased fluidity of membranes prepared from diabetic subjects. The decreased fluidity of diabetic membra
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