Relevant bibliographies by topics / Fluorescence Polarization Microscopy

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic 'Fluorescence Polarization Microscopy'

Author: Grafiati
Published: 4 June 2021
Last updated: 6 February 2022

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Journal articles on the topic "Fluorescence Polarization Microscopy"

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Zhanghao, Karl, Juntao Gao, Dayong Jin, Xuedian Zhang, and Peng Xi. "Super-resolution fluorescence polarization microscopy." Journal of Innovative Optical Health Sciences 11, no. 01 (2017): 1730002. http://dx.doi.org/10.1142/s1793545817300026.

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Fluorescence polarization is related to the dipole orientation of chromophores, making fluorescence polarization microscopy possible to reveal structures and functions of tagged cellular organelles and biological macromolecules. Several recent super resolution techniques have been applied to fluorescence polarization microscopy, achieving dipole measurement at nanoscale. In this review, we summarize both diffraction limited and super resolution fluorescence polarization microscopy techniques, as well as their applications in biological imaging.
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Nakai, Nori, Keisuke Sato, Tomomi Tani, Kenta Saito, Fumiya Sato, and Sumio Terada. "Genetically encoded orientation probes for F-actin for fluorescence polarization microscopy." Microscopy 68, no. 5 (2019): 359–68. http://dx.doi.org/10.1093/jmicro/dfz022.

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Abstract Fluorescence polarization microscopy, which can visualize both position and orientation of fluorescent molecules, is useful for analyzing architectural dynamics of proteins in vivo, especially that of cytoskeletal proteins such as actin. Fluorescent phalloidin conjugates and SiR-actin can be used as F-actin orientation probes for fluorescence polarization microscopy, but a lack of appropriate methods for their introduction to living specimens especially to tissues, embryos, and whole animals hampers their applications to image the orientation of F-actin. To solve this problem, we have
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Bokor, Nándor, Yoshinori Iketaki, Takeshi Watanabe, Kota Daigoku, Nir Davidson, and Masaaki Fujii. "On polarization effects in fluorescence depletion microscopy." Optics Communications 272, no. 1 (2007): 263–68. http://dx.doi.org/10.1016/j.optcom.2006.11.002.

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Wang, Nan, and Takayoshi Kobayashi. "Polarization modulation for fluorescence emission difference microscopy." Optics Express 23, no. 10 (2015): 13704. http://dx.doi.org/10.1364/oe.23.013704.

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Sheppard, C. J. R., and P. Török. "An electromagnetic theory of imaging in fluorescence microscopy, and imaging in polarization fluorescence microscopy." Bioimaging 5, no. 4 (1997): 205–18. http://dx.doi.org/10.1002/1361-6374(199712)5:4<205::aid-bio4>3.3.co;2-v.

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Rehman, Khalil Ur, Subir Das, and Fu-Jen Kao. "High-contrast fluorescence polarization microscopy through stimulated emission." Applied Physics Express 14, no. 2 (2021): 022008. http://dx.doi.org/10.35848/1882-0786/abdc9d.

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Liu, Yang, Timothy York, Walter Akers, Gail Sudlow, Viktor Gruev, and Samuel Achilefu. "Complementary fluorescence-polarization microscopy using division-of-focal-plane polarization imaging sensor." Journal of Biomedical Optics 17, no. 11 (2012): 116001. http://dx.doi.org/10.1117/1.jbo.17.11.116001.

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Chacko, Jenu V., Han Nim Lee, Wenxin Wu, Marisa S. Otegui, and Kevin W. Eliceiri. "Hyperdimensional Imaging Contrast Using an Optical Fiber." Sensors 21, no. 4 (2021): 1201. http://dx.doi.org/10.3390/s21041201.

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Fluorescence properties of a molecule can be used to study the structural and functional nature of biological processes. Physical properties, including fluorescence lifetime, emission spectrum, emission polarization, and others, help researchers probe a molecule, produce desired effects, and infer causes and consequences. Correlative imaging techniques such as hyperdimensional imaging microscopy (HDIM) combine the physical properties and biochemical states of a fluorophore. Here we present a fiber-based imaging system that can generate hyper-dimensional contrast by combining multiple fluoresce
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Luchowski, Rafal, Pabak Sarkar, Shashank Bharill, et al. "Fluorescence polarization standard for near infrared spectroscopy and microscopy." Applied Optics 47, no. 33 (2008): 6257. http://dx.doi.org/10.1364/ao.47.006257.

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Goto, A., T. Ohba, and K. Ohki. "Fluorescence polarization imaging microscopy by using Mueller matrix method." Seibutsu Butsuri 39, supplement (1999): S208. http://dx.doi.org/10.2142/biophys.39.s208_3.

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Dissertations / Theses on the topic "Fluorescence Polarization Microscopy"

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Valadés, Cruz César Augusto. "Polarized super-resolution fluorescence microscopy." Thesis, Aix-Marseille, 2014. http://www.theses.fr/2014AIXM4333.

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Alors que la microscopie super-résolue a apporté une amélioration considérable en imagerie des assemblages moléculaires dans les milieux biologiques à l'échelle nanométrique, son extension à l'imagerie de l'orientation moléculaire, utilisant l'anisotropie de fluorescence, n'a pas encore été complètement explorée. Apporter une information sur l'orientation moléculaire à l'échelle nanométrique aurait un intérêt considérable pour la compréhension des fonctions biologiques. Dans cette thèse, nous proposons une technique de microscopie super-résolution résolue en polarisation, capable d'imager les
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Reitz, Frederick B. "Fluorescence anisotropy near-field scanning optical microscopy (FANSOM) : a new technique for biological microviscometry /." Thesis, Connect to this title online; UW restricted, 2001. http://hdl.handle.net/1773/8098.

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Valadés, Cruz César Augusto. "Polarized super-resolution fluorescence microscopy." Doctoral thesis, Universitat Politècnica de Catalunya, 2014. http://hdl.handle.net/10803/277565.

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While super-resolution microscopy has brought a significant improvement in nano-scale imaging of molecular assemblies in biological media, its extension to imaging molecular orientation using fluorescence anisotropy has not yet been fully explored. Providing orientational order information at the nano-scale would be of considerable interest for the understanding of biological functions since they are intrinsically related to structural fundamental processes such as in protein clustering in cell membranes, supra-molecular polymerization or aggregation. In this thesis, we propose a super-resolut
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Kwok, Ka Cheung. "Measuring binding kinetics of ligands with tethered receptors by fluorescence polarization complemented with total internal reflection fluorescence microscopy." HKBU Institutional Repository, 2010. https://repository.hkbu.edu.hk/etd_oa/18.

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The study of the binding between estrogen receptors (ER) and their ligands in vitro has long been of interest mainly because of its application in anti-estrogen drug discovery for breast cancer treatment as well as in the screening of environmental contaminants for endocrine disruptors. Binding strength was conventionally quantified in terms of equilibrium dissociation constant (KD). Recently, emphasis is shifting towards kinetics rate constants, and off-rate (koff) in particular. This thesis reported a novel method to measure such binding kinetics based on fluorescence polarization complement
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Schäfer, Philip Sudadyo. "Tuning of color and polarization of the fluorescence of nano-ribbons using laser microscopy and controlled self-assembly." Thesis, Bordeaux, 2016. http://www.theses.fr/2016BORD0435/document.

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Des matériaux ayant des propriétés émissives spécifiques peuvent être obtenus par l'organisation contrôlée de fluorophores aux échelles moléculaire, nano- et micro-métrique. Dans ce travail, l'émission de lumière bleue polarisée est obtenue par l'auto-assemblage hautement anisotrope de n-acènes alcoxylés en nano-rubans. Des techniques de microscopie de fluorescence ont été utilisées pour déterminer le mécanisme de leur croissance et ont été combinées à la cristallographie aux rayons X pour déterminer l'empilement moléculaire dans les nano-objets. L'étude a révélé que la formation des nano-ruba
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Yiu, Kwok Wing. "Measuring the binding between estrogen receptor alpha and potential endocrine disruptors by fluorescence polarization and total internal reflection fluorescence." HKBU Institutional Repository, 2013. http://repository.hkbu.edu.hk/etd_ra/1503.

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Ahmed, Haitham Ahmed Shaban. "Quantitative molecular orientation imaging of biological structures by polarized super-resolution fluorescence microscopy." Thesis, Aix-Marseille, 2015. http://www.theses.fr/2015AIXM4323.

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Dans cette thèse, nous avons construit et optimisé des méthodes de microscopie de fluorescence super-résolue stochastique, polarisée et quantitative qui nous permettent d'imager l'orientation moléculaire dans des environnements dynamiques et statiques a l’échelle de la molécule unique et avec une résolution nanoscopique. En utilisant un montage de microscopie super-résolue à lecture stochastique en combinaison avec une détection polarisée, nous avons pu reconstruire des images d'anisotropie de fluorescence avec une résolution spatiale de 40 nm. En particulier, nous avons pu imager l'ordre orie
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Pumpa, Martin. "Single Molecule Diffusion in Liquid Crystals." Doctoral thesis, Universitätsbibliothek Leipzig, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-157717.

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The present work introduces a new method that is used to explore the connection between molecular order and molecular dynamics in liquid crystals. In liquid crystals, the building blocks show a liquid like disorder in at least one dimension of space with an otherwise crystalline like positional or orientational long range order. A new microscope is introduced that combines polarization measurements with the ability to track single fluorescent probe molecules in a thin sample of ordered liquid crystal. A new method for the analysis of orientation dependent diffusion is also introduced. It can b
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Matsuzaki, Satoshi. "Hole Burning Imaging Studies of Cancerous and Analogous Normal Ovarian Tissues Utilizing Organelle Specific Dyes." Ames, Iowa : Oak Ridge, Tenn. : Ames Laboratory ; distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2004. http://www.osti.gov/servlets/purl/837275-3aN4nd/webviewable/.

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Thesis (Ph.D.); Submitted to Iowa State Univ., Ames, IA (US); 19 Dec 2004.<br>Published through the Information Bridge: DOE Scientific and Technical Information. "IS-T 2692" Satoshi Matsuzaki. US Department of Energy 12/19/2004. Report is also available in paper and microfiche from NTIS.
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Gozhyk, Iryna. "Polarization and gain phenomena in dye-doped polymer micro-lasers." Phd thesis, École normale supérieure de Cachan - ENS Cachan, 2012. http://tel.archives-ouvertes.fr/tel-01063044.

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The demonstration of an electrically pumped organic laser remains a major issue of organic optoelectronics for several decades. This goal requires an improved device configuration so as to reduce losses which are intrinsically higher under electrical excitation compared to optical pumping. Moreover a systematic investigation of the material properties is still missing and should lead to a reliable estimate of the lasing threshold under optical pumping, and then to a lower limit for electrical pumping. In this thesis we addressed the issue of gain and polarization properties of organic material
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Book chapters on the topic "Fluorescence Polarization Microscopy"

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Brasselet, Sophie, Patrick Ferrand, Alla Kress, Xiao Wang, Hubert Ranchon, and Alicja Gasecka. "Imaging Molecular Order in Cell Membranes by Polarization-Resolved Fluorescence Microscopy." In Springer Series on Fluorescence. Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/4243_2012_51.

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Benoit, Matthieu P. M. H., and Hernando Sosa. "Use of Single Molecule Fluorescence Polarization Microscopy to Study Protein Conformation and Dynamics of Kinesin–Microtubule Complexes." In Single Molecule Analysis. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7271-5_11.

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Bensimon, David, Vincent Croquette, Jean-François Allemand, Xavier Michalet, and Terence Strick. "Fluorescence Spectroscopy and Microscopy Techniques." In Single-Molecule Studies of Nucleic Acids and Their Proteins. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198530923.003.0003.

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This chapter reviews the use of fluorescent methods in the study of single molecules, how the foundations of fluorescence are rooted in Einstein’s description of absorption and emission (spontaneous and stimulated), and their quantum-mechanical explanation in terms of transitions between quantized energy levels, as represented in Jablonski diagrams. It describes the non-radiative channels which compete with fluorescent emission, decrease its efficiency, and ultimately destroy the fluorescent molecule. Fluorescence Polarization Spectroscopy, FRET, and FCS are briefly presented. Without reviewing the various available fluorophores, it describes the various illumination methods used to study them, sketching super-resolution methods (STED, STORM, PALM) that have recently allowed fluorophores to be resolved to a few tens of nanometres. Finally, it describes the considerations (bandwidth, signal to noise, signal to background) used in choosing a single-molecule fluorescence detector, and the extraction of the diffusion constant of a fluorophore from the finite time, noisy traces of its positions.
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Piston, David W., and Mark A. Rizzo. "FRET by Fluorescence Polarization Microscopy." In Fluorescent Proteins. Elsevier, 2008. http://dx.doi.org/10.1016/s0091-679x(08)85018-2.

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Axelrod, Daniel. "Chapter 12 Fluorescence Polarization Microscopy." In Methods in Cell Biology. Elsevier, 1989. http://dx.doi.org/10.1016/s0091-679x(08)60985-1.

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Yan, Yuling, and Gerard Marriott. "[24] Fluorescence resonance energy transfer imaging microscopy and fluorescence polarization imaging microscopy." In Methods in Enzymology. Elsevier, 2003. http://dx.doi.org/10.1016/s0076-6879(03)60128-x.

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Sosa, Hernando, Ana B. Asenjo, and Erwin J. G. Peterman. "Structure and Dynamics of the Kinesin–Microtubule Interaction Revealed by Fluorescence Polarization Microscopy." In Methods in Cell Biology. Elsevier, 2010. http://dx.doi.org/10.1016/s0091-679x(10)95025-5.

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Lindsay Bashford, C. "Spectrophotometry and fluorimetry of cellular compartments and Intracellular processes." In Spectrophotometry and Spectrofluorimetry. Oxford University Press, 2000. http://dx.doi.org/10.1093/oso/9780199638130.003.0015.

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Optical spectroscopy, Spectrophotometry and fluorimetry can be used to monitor processes occurring in living cells provided that suitable chromophores are present which ‘report’ on the events in which they participate. The advantages of optical techniques are manifold. Firstly they can be fast—with appropriate apparatus events in the pico- and nano-second domains can be studied by fluorescence spectroscopy. Secondly they are continuous—instant feedback from the experimental system can guide the most complex of experimental protocols, and allow the experimenter to adjust system parameters as necessary. Thirdly they are convenient, and most laboratories have access to equipment that can provide quantitative analysis of optical signals; examples include conventional spectrophotometers/fluorimeters, dedicated instruments (e.g. for fluorescence lifetime and polarization measurements), cameras, microscopes and plate readers. Significantly detectors from one apparatus can often be used on others to open up new experimental protocols. Fortunately the principles underlying the use of such a diverse array of optical devices are straightforward and universal—they apply just as much to laboratory ‘work-horse’ instruments as they do to the most specialized, laser-illuminated fluorescence microscope. The availability of fast laboratory computers with large storage capacities means that most modern spectrometers are microprocessor controlled and digitization of signals opens up the full range of possibilities of data accumulation, storage, analysis and interpretation. The main problem with optical measurements is not the acquisition but rather the interpretation of the data obtained. Straightforward analysis of the results depends on the clarity of the experimental design and the appropriate choice of chromophore. This chapter describes some of the problems that can be addressed by spectroscopic techniques and attempts to give guidance on good experimental design. Optical spectroscopy requires either spectrophotometers, to measure absorbance, fluorimeters, to measure fluorescence, or microscopes, which can measure fluorescence or absorbance of single cells or small groups of cells. Fluorimeters and spectrophotometers usually require solutions or suspensions of material in conventional cuvettes; microscopes provide two-dimensional images from smears, slices or surfaces. Other devices that record signals resolved in two-dimensions include gel scanners and microplate readers.
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Mark, James E., Dale W. Schaefer, and Gui Lin. "Some Characterization Techniques Useful for Polysiloxanes." In The Polysiloxanes. Oxford University Press, 2015. http://dx.doi.org/10.1093/oso/9780195181739.003.0006.

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The general approach used in choosing a polymer suitable for a particular application is: . . . Polymerization ↔ Structure ↔ Properties ↔ Application . . . For example, if one wants a polymer for fire-resistant fabrics, then a polymer with good high-temperature properties is required, which implies aromatic structures, which suggest condensation polymerizations. More relevant here, however, would be that a polymer remains elastomeric at low temperatures. This requirement evokes a polymer with high flexibility (low glass transition temperature), which indicates use of the polymerization techniques used with the polysiloxanes. An example of a relevant optical property is the birefringence of a deformed polymer network. This strain-induced birefringence can be used to characterize segmental orientation, and both Gaussian and non-Gaussian elasticity. Infrared dichroism has also been helpful in this regard. In the case of the crystallizable polysiloxane elastomers, orientation is of critical importance with regard to strain-induced crystallization and the tremendous reinforcement it provides. Segmental orientation has also been characterized by fluorescence polarization, deuterium nuclear magnetic resonance (NMR), and polarized infrared spectroscopy. Infrared spectroscopy has been used to characterize the structures of silica-filled polydimethylsiloxane (PDMS). Other optical and spectroscopic techniques are also important, including positron annihilation lifetime spectroscopy, spectroscopic ellipsometry, confocal Raman spectroscopy, and photoluminescence spectroscopy. Surface-enhanced Raman spectroscopy has been made tunable using gold nanorods and strain control on elastomeric PDMS substrates. A great deal of information is now being obtained on filler dispersion and other aspects of elastomer structure and morphology through the use of scanning probe microscopy, which consists of several approaches. One approach is that of scanning tunneling microscopy (STM), in which an extremely sharp metal tip on a cantilever is passed along the surface while measuring the electric current flowing through quantum mechanical tunneling. Monitoring the current then permits maintaining the probe at a fixed height above the surface. Display of probe height as a function of surface coordinates then gives the desired topographic map. One limitation of this approach is the requirement that the sample be electrically conductive. Atomic force microscopy (AFM), on the other hand, does not require a conducting Surface.
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Conference papers on the topic "Fluorescence Polarization Microscopy"

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de Vito, Giuseppe. "The importance of the excitation light polarization state for the optimization of the signal levels in two-photon light-sheet microscopy." In Virtual 12th Light Sheet Fluorescence Microscopy Conference 2020. Royal Microscopical Society, 2020. http://dx.doi.org/10.22443/rms.lsfm2020.11.

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Bigelow, Chad E., John G. Frelinger, and Thomas H. Foster. "Enzyme activity imaging with confocal fluorescence polarization microscopy." In Biomedical Topical Meeting. OSA, 2004. http://dx.doi.org/10.1364/bio.2004.fh14.

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Gnanatheepam, Einstein, Anandh Sundaramoorthy, Bharanidharan Ganesan, et al. "Live cell metabolic imaging of cancer cell lines using multiphoton fluorescence polarization." In Multiphoton Microscopy in the Biomedical Sciences XX, edited by Ammasi Periasamy, Peter T. So, and Karsten König. SPIE, 2020. http://dx.doi.org/10.1117/12.2545847.

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Roy, Debjit, Arijit Kumar De, and Debabrata Goswami. "Polarization modulated Ultrafast Pulse-Pair Control in Two-Photon Fluorescence Microscopy." In Frontiers in Optics. OSA, 2011. http://dx.doi.org/10.1364/fio.2011.jwa3.

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Lee, Ji Youn, John F. Lesoine, Jeffrey R. Krogmeier, et al. "Real-time fluorescence polarization microscopy for probing local distributions of biomolecules." In SPIE BiOS, edited by Ramesh Raghavachari and Rongguang Liang. SPIE, 2011. http://dx.doi.org/10.1117/12.875612.

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Täuber, Daniela. "Imaging aggregation of histologically stained F-actin ex vivo using the contrast in Förster resonance energy transfer obtained from 2D polarization fluorescence imaging (2D-POLIM)." In European Light Microscopy Initiative 2021. Royal Microscopical Society, 2021. http://dx.doi.org/10.22443/rms.elmi2021.6.

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Hassanzadeh, Abdollah, Ata Bahmani, and Silvia Mittler. "Variable angle total internal reflection fluorescence microscopy in s-polarization: a new approach to quantify cell-substrate distances in contacts." In Photonics North 2010, edited by Henry P. Schriemer and Rafael N. Kleiman. SPIE, 2010. http://dx.doi.org/10.1117/12.872938.

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Chang, Hsueh-Chia. "Electrokinetics of Nanochannels: The Next Nanotechnology for Ion/Molecule/Nanocolloid Sensing, Concentration and Filtration." In ASME 2009 7th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2009. http://dx.doi.org/10.1115/icnmm2009-82128.

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Because nanochannels act as lenses that can focus electric fields on a chip, they can concentrate and filter ions, molecules and nanocolloids by ion selectivity, electrodeless dielectrophoresis and surface hybridization. However, before fabricated nanochannels can lead to the next-generation of chip-scale dialysis membranes, preconcentrators for mass spectrometers, ion sieves, molecular sensors, solar/fuel cell membranes etc, the anomalous dc I-V characteristics and ac impedance of such nanochannels must be understood. Curious phenomena such as limiting and over-limiting currents, rectificatio
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Tatsumi, Kazuya, Akihisa Tozaki, and Kazuyoshi Nakabe. "Microscopic Fluid Temperature Measurements Using Fluorescence Polarization Method." In ASME/JSME 2011 8th Thermal Engineering Joint Conference. ASMEDC, 2011. http://dx.doi.org/10.1115/ajtec2011-44461.

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A fluid temperature measurement method in microscopic scale using fluorescence polarization is described in this study. The present method has the advantages in not only noncontact but also markedly reducing the influences of solution pH and quenching on the measured fluid temperature, compared to other methods including LIF (laser induced fluorescence) method. In the case of a fluid at rest, the fluorescence intensity varied with pH and linearly decreased with the elapsed time, while the polarization degree remained nearly constant. The polarization degree showed a good correlation with the f
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Franco, Egberto Gomes, Paulo Lucas Dantas Filho, Carlos Eduardo Rollo Ribeiro, Geraldo Francisco Burani, and Marcelo Linardi. "Proton Exchange Membrane Fuel Cell Catalyst: Synthesis and Characterization." In ASME 2008 6th International Conference on Fuel Cell Science, Engineering and Technology. ASMEDC, 2008. http://dx.doi.org/10.1115/fuelcell2008-65068.

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Fuel cells are a promising technology to deal with energy sustainability, especially for mobility purposes the Proton Exchange Membrane Fuel Cell and hydrogen produced from biomass could be coupled to overcome the amount of CO2 emissions. In order to improve fuel cells performances the search for new electrocatalysts has a great importance in this technology the challenge for a fuel cell catalyst that is less poisoned by CO is one of the most important field in low temperature fuel cell developments that use alcohol and hydrocarbons as primary fuels. In this work PtSm, PtTb, PtDy, PtU, PtRuMo
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