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Relevant bibliographies by topics / Fluorescence Polarization Microscopy / Dissertations / Theses

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Dissertations / Theses on the topic 'Fluorescence Polarization Microscopy'

Author: Grafiati

Published: 4 June 2021

Last updated: 6 February 2022

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1

Valadés, Cruz César Augusto. "Polarized super-resolution fluorescence microscopy." Thesis, Aix-Marseille, 2014. http://www.theses.fr/2014AIXM4333.

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Alors que la microscopie super-résolue a apporté une amélioration considérable en imagerie des assemblages moléculaires dans les milieux biologiques à l'échelle nanométrique, son extension à l'imagerie de l'orientation moléculaire, utilisant l'anisotropie de fluorescence, n'a pas encore été complètement explorée. Apporter une information sur l'orientation moléculaire à l'échelle nanométrique aurait un intérêt considérable pour la compréhension des fonctions biologiques. Dans cette thèse, nous proposons une technique de microscopie super-résolution résolue en polarisation, capable d'imager les

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2

Reitz, Frederick B. "Fluorescence anisotropy near-field scanning optical microscopy (FANSOM) : a new technique for biological microviscometry /." Thesis, Connect to this title online; UW restricted, 2001. http://hdl.handle.net/1773/8098.

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3

Valadés, Cruz César Augusto. "Polarized super-resolution fluorescence microscopy." Doctoral thesis, Universitat Politècnica de Catalunya, 2014. http://hdl.handle.net/10803/277565.

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While super-resolution microscopy has brought a significant improvement in nano-scale imaging of molecular assemblies in biological media, its extension to imaging molecular orientation using fluorescence anisotropy has not yet been fully explored. Providing orientational order information at the nano-scale would be of considerable interest for the understanding of biological functions since they are intrinsically related to structural fundamental processes such as in protein clustering in cell membranes, supra-molecular polymerization or aggregation. In this thesis, we propose a super-resolut

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4

Kwok, Ka Cheung. "Measuring binding kinetics of ligands with tethered receptors by fluorescence polarization complemented with total internal reflection fluorescence microscopy." HKBU Institutional Repository, 2010. https://repository.hkbu.edu.hk/etd_oa/18.

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The study of the binding between estrogen receptors (ER) and their ligands in vitro has long been of interest mainly because of its application in anti-estrogen drug discovery for breast cancer treatment as well as in the screening of environmental contaminants for endocrine disruptors. Binding strength was conventionally quantified in terms of equilibrium dissociation constant (KD). Recently, emphasis is shifting towards kinetics rate constants, and off-rate (koff) in particular. This thesis reported a novel method to measure such binding kinetics based on fluorescence polarization complement

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5

Schäfer, Philip Sudadyo. "Tuning of color and polarization of the fluorescence of nano-ribbons using laser microscopy and controlled self-assembly." Thesis, Bordeaux, 2016. http://www.theses.fr/2016BORD0435/document.

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Des matériaux ayant des propriétés émissives spécifiques peuvent être obtenus par l'organisation contrôlée de fluorophores aux échelles moléculaire, nano- et micro-métrique. Dans ce travail, l'émission de lumière bleue polarisée est obtenue par l'auto-assemblage hautement anisotrope de n-acènes alcoxylés en nano-rubans. Des techniques de microscopie de fluorescence ont été utilisées pour déterminer le mécanisme de leur croissance et ont été combinées à la cristallographie aux rayons X pour déterminer l'empilement moléculaire dans les nano-objets. L'étude a révélé que la formation des nano-ruba

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6

Yiu, Kwok Wing. "Measuring the binding between estrogen receptor alpha and potential endocrine disruptors by fluorescence polarization and total internal reflection fluorescence." HKBU Institutional Repository, 2013. http://repository.hkbu.edu.hk/etd_ra/1503.

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7

Ahmed, Haitham Ahmed Shaban. "Quantitative molecular orientation imaging of biological structures by polarized super-resolution fluorescence microscopy." Thesis, Aix-Marseille, 2015. http://www.theses.fr/2015AIXM4323.

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Dans cette thèse, nous avons construit et optimisé des méthodes de microscopie de fluorescence super-résolue stochastique, polarisée et quantitative qui nous permettent d'imager l'orientation moléculaire dans des environnements dynamiques et statiques a l’échelle de la molécule unique et avec une résolution nanoscopique. En utilisant un montage de microscopie super-résolue à lecture stochastique en combinaison avec une détection polarisée, nous avons pu reconstruire des images d'anisotropie de fluorescence avec une résolution spatiale de 40 nm. En particulier, nous avons pu imager l'ordre orie

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8

Pumpa, Martin. "Single Molecule Diffusion in Liquid Crystals." Doctoral thesis, Universitätsbibliothek Leipzig, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-157717.

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The present work introduces a new method that is used to explore the connection between molecular order and molecular dynamics in liquid crystals. In liquid crystals, the building blocks show a liquid like disorder in at least one dimension of space with an otherwise crystalline like positional or orientational long range order. A new microscope is introduced that combines polarization measurements with the ability to track single fluorescent probe molecules in a thin sample of ordered liquid crystal. A new method for the analysis of orientation dependent diffusion is also introduced. It can b

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9

Matsuzaki, Satoshi. "Hole Burning Imaging Studies of Cancerous and Analogous Normal Ovarian Tissues Utilizing Organelle Specific Dyes." Ames, Iowa : Oak Ridge, Tenn. : Ames Laboratory ; distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2004. http://www.osti.gov/servlets/purl/837275-3aN4nd/webviewable/.

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Thesis (Ph.D.); Submitted to Iowa State Univ., Ames, IA (US); 19 Dec 2004.<br>Published through the Information Bridge: DOE Scientific and Technical Information. "IS-T 2692" Satoshi Matsuzaki. US Department of Energy 12/19/2004. Report is also available in paper and microfiche from NTIS.

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10

Gozhyk, Iryna. "Polarization and gain phenomena in dye-doped polymer micro-lasers." Phd thesis, École normale supérieure de Cachan - ENS Cachan, 2012. http://tel.archives-ouvertes.fr/tel-01063044.

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The demonstration of an electrically pumped organic laser remains a major issue of organic optoelectronics for several decades. This goal requires an improved device configuration so as to reduce losses which are intrinsically higher under electrical excitation compared to optical pumping. Moreover a systematic investigation of the material properties is still missing and should lead to a reliable estimate of the lasing threshold under optical pumping, and then to a lower limit for electrical pumping. In this thesis we addressed the issue of gain and polarization properties of organic material

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11

Östlund, Helena. "Evaluating the Congo red staining method with the aim to solve problematics in the work process and optimize amyloidosis diagnostics." Thesis, Uppsala universitet, Institutionen för kvinnors och barns hälsa, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-327122.

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Some diagnostic methods have been used for a very long time. Congo red stain saw the light of day in 1883, and quickly became important in many fields of use. Nowadays we recognize the importance of Congo red in diagnose of amyloid diseases. However, the technique and experience needed throughout the process from a suspected case to the diagnose is of greate importance. When diagnostic difficulties appeared in a few patient cases at the local hospital in Gävle, Sweden, a solution was needed. A delayed diagnose could have a potential devastating outcome seen in the perspective of the patient. T

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12

Weng, Shu-Chuan. "Preclinical exploration of novel small molecules as anticancer agents in triple-negative and HER2/neu-positive breast cancers." The Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1227727553.

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13

Tsai, Han-Ruei, and 蔡涵睿. "Molecular Dynamics Revealed by Time-Resolved Fluorescence Polarization Microscopy." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/38517619490869048073.

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碩士<br>國立陽明大學<br>生醫光電研究所<br>100<br>There are two mathematics describing polarization state, one is Jones’ calculus and other is Stokes parameters. Jones’ calculus only can describe the fully polarization state. On the contrary, Stokes parameters not only describe the perfectly polarized light, but also describe the partially polarized light and unpolarized light. In this thesis, we have developed a four-channel Stokes vector formalism based fluorescence polarization microscope with lifetime imaging to characterize the polarization state of fluorescence. The polarimeter was demonstrated by a s

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14

(6331859), Changqin Ding. "Polarization-enabled Multidimensional Optical Microscopy." Thesis, 2019.

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Polarization-dependence provides a unique handle for extending the dimensionality of optical microscopy, with particular benefits in nonlinear optical imaging. Polarization-dependent second order nonlinear optical processes such as second harmonic generation (SHG) provide rich qualitative and quantitative information on local molecular orientation distribution. By bridging Mueller and Jones tensor, a theoretical framework was introduced to experimentally extend the application of polarization-dependent SHG microscopy measurements toward in vivo imaging, in which partial polarization or depolar

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15

Lo, Jocelyn. "Homo-FRET Imaging of CEACAM1 in Living Cells using Total Internal Reflection Fluorescence Polarization Microscopy." Thesis, 2012. http://hdl.handle.net/1807/33307.

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Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) undergoes homotypic and heterotypic cis- and trans- interactions that regulate processes including metabolism, immune response, and tumorigenesis. To better understand and eventually control CEACAM1’s numerous roles, we characterized the localization, homotypic cis-oligomerization, and regulation of CEACAM1 at the molecular scale using steady-state TIRFPM homo-FRET imaging in living cells. We established the anisotropy sensitivity of our TIRFPM platform using Venus monomers and dimers, which had significantly different anisot

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16

Oreopoulos, John. "Combinatorial Microscopy of Molecular Interactions at Membrane Interfaces." Thesis, 2011. http://hdl.handle.net/1807/27598.

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Biological membranes are heterogeneous two-dimensional fluids composed of lipids, sterols and proteins that act as complex gateways and define the cell boundary. The functions of these interfaces are diverse and specific to individual organisms, cell types, and tissues. Membranes must take up nutrients and small molecules, release waste products, bind ligands, transmit signals, convert energy, sense the environment, maintain cell adhesion, control cell migration, and much more while forming a tight barrier around the cell. The molecular mechanisms and structural details responsible for this di

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17

(10711971), Alex M. Sherman. "Dynamic Chemical Imaging And Analysis Within Biologically Active Materials." Thesis, 2021.

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A thorough understanding of pharmaceutical and therapeutic products and materials is important for an improved quality of life. By probing the complex behaviors and properties of these systems, new insights can allow for a better understanding of current treatments, improved design and synthesis of new drug products, and the development of new treatments for various health conditions. Often, the impact of these new insights are limited by current technology and instrumentation and by the methods in which existing data is processed. Additionally,

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18

(8713962), James Ulcickas. "LIGHT AND CHEMISTRY AT THE INTERFACE OF THEORY AND EXPERIMENT." Thesis, 2020.

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Optics are a powerful probe of chemical structure that can often be linked to theoretical predictions, providing robustness as a measurement tool. Not only do optical interactions like second harmonic generation (SHG), single and two-photon excited fluorescence (TPEF), and infrared absorption provide chemical specificity at the molecular and macromolecular scale, but the ability to image enables mapping heterogeneous behavior across complex systems such as biological tissue. This thesis will discuss nonlinear and linear optics, leveraging theoretical predictions to provide frameworks for inter

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