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Journal articles on the topic 'Fluorescence Polarization Microscopy'

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1

Zhanghao, Karl, Juntao Gao, Dayong Jin, Xuedian Zhang, and Peng Xi. "Super-resolution fluorescence polarization microscopy." Journal of Innovative Optical Health Sciences 11, no. 01 (2017): 1730002. http://dx.doi.org/10.1142/s1793545817300026.

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Fluorescence polarization is related to the dipole orientation of chromophores, making fluorescence polarization microscopy possible to reveal structures and functions of tagged cellular organelles and biological macromolecules. Several recent super resolution techniques have been applied to fluorescence polarization microscopy, achieving dipole measurement at nanoscale. In this review, we summarize both diffraction limited and super resolution fluorescence polarization microscopy techniques, as well as their applications in biological imaging.
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2

Nakai, Nori, Keisuke Sato, Tomomi Tani, Kenta Saito, Fumiya Sato, and Sumio Terada. "Genetically encoded orientation probes for F-actin for fluorescence polarization microscopy." Microscopy 68, no. 5 (2019): 359–68. http://dx.doi.org/10.1093/jmicro/dfz022.

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Abstract Fluorescence polarization microscopy, which can visualize both position and orientation of fluorescent molecules, is useful for analyzing architectural dynamics of proteins in vivo, especially that of cytoskeletal proteins such as actin. Fluorescent phalloidin conjugates and SiR-actin can be used as F-actin orientation probes for fluorescence polarization microscopy, but a lack of appropriate methods for their introduction to living specimens especially to tissues, embryos, and whole animals hampers their applications to image the orientation of F-actin. To solve this problem, we have
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3

Bokor, Nándor, Yoshinori Iketaki, Takeshi Watanabe, Kota Daigoku, Nir Davidson, and Masaaki Fujii. "On polarization effects in fluorescence depletion microscopy." Optics Communications 272, no. 1 (2007): 263–68. http://dx.doi.org/10.1016/j.optcom.2006.11.002.

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4

Wang, Nan, and Takayoshi Kobayashi. "Polarization modulation for fluorescence emission difference microscopy." Optics Express 23, no. 10 (2015): 13704. http://dx.doi.org/10.1364/oe.23.013704.

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5

Sheppard, C. J. R., and P. Török. "An electromagnetic theory of imaging in fluorescence microscopy, and imaging in polarization fluorescence microscopy." Bioimaging 5, no. 4 (1997): 205–18. http://dx.doi.org/10.1002/1361-6374(199712)5:4<205::aid-bio4>3.3.co;2-v.

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6

Rehman, Khalil Ur, Subir Das, and Fu-Jen Kao. "High-contrast fluorescence polarization microscopy through stimulated emission." Applied Physics Express 14, no. 2 (2021): 022008. http://dx.doi.org/10.35848/1882-0786/abdc9d.

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7

Liu, Yang, Timothy York, Walter Akers, Gail Sudlow, Viktor Gruev, and Samuel Achilefu. "Complementary fluorescence-polarization microscopy using division-of-focal-plane polarization imaging sensor." Journal of Biomedical Optics 17, no. 11 (2012): 116001. http://dx.doi.org/10.1117/1.jbo.17.11.116001.

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8

Chacko, Jenu V., Han Nim Lee, Wenxin Wu, Marisa S. Otegui, and Kevin W. Eliceiri. "Hyperdimensional Imaging Contrast Using an Optical Fiber." Sensors 21, no. 4 (2021): 1201. http://dx.doi.org/10.3390/s21041201.

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Fluorescence properties of a molecule can be used to study the structural and functional nature of biological processes. Physical properties, including fluorescence lifetime, emission spectrum, emission polarization, and others, help researchers probe a molecule, produce desired effects, and infer causes and consequences. Correlative imaging techniques such as hyperdimensional imaging microscopy (HDIM) combine the physical properties and biochemical states of a fluorophore. Here we present a fiber-based imaging system that can generate hyper-dimensional contrast by combining multiple fluoresce
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9

Luchowski, Rafal, Pabak Sarkar, Shashank Bharill, et al. "Fluorescence polarization standard for near infrared spectroscopy and microscopy." Applied Optics 47, no. 33 (2008): 6257. http://dx.doi.org/10.1364/ao.47.006257.

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10

Goto, A., T. Ohba, and K. Ohki. "Fluorescence polarization imaging microscopy by using Mueller matrix method." Seibutsu Butsuri 39, supplement (1999): S208. http://dx.doi.org/10.2142/biophys.39.s208_3.

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11

BIGELOW, CHAD E., HARSHAD D. VISHWASRAO, JOHN G. FRELINGER, and THOMAS H. FOSTER. "Imaging enzyme activity with polarization-sensitive confocal fluorescence microscopy." Journal of Microscopy 215, no. 1 (2004): 24–33. http://dx.doi.org/10.1111/j.0022-2720.2004.01357.x.

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12

Dean, William F., Emily I. Bartle, and Alexa L. Mattheyses. "Cadherin Organization in Desmosomes Probed using Fluorescence Polarization Microscopy." Biophysical Journal 118, no. 3 (2020): 308a—309a. http://dx.doi.org/10.1016/j.bpj.2019.11.1742.

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13

Sakhi, Alina, and Josef Lazar. "Observing Tyrosine Kinase Function by Polarization Resolved Fluorescence Microscopy." Biophysical Journal 120, no. 3 (2021): 327a. http://dx.doi.org/10.1016/j.bpj.2020.11.2061.

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14

Hayashi, Terutake, Masaki Michihata, Yasuhiro Takaya, and Kok Foong Lee. "Development of Nano Particle Sizing System Using Fluorescence Polarization." ACTA IMEKO 2, no. 2 (2014): 67. http://dx.doi.org/10.21014/acta_imeko.v2i2.108.

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&lt;p&gt;In order to measure the sizes of nanoparticles with a wide size distribution in a solvent, we developed an optical microscopy system that allows for fluorescence polarization (FP) measurement and optical observation. This system allows the evaluation of nanoparticle sizes over a wide range, because the fluorescent signal intensity is independent of changes in the nanoparticle sizes. In this paper, we describe a fundamental experiment to verify the feasibility of using this system for different sizes of nanoparticles.&lt;/p&gt;
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15

Zondervan, Rob, Florian Kulzer, Harmen van der Meer, Jos A. J. M. Disselhorst, and Michel Orrit. "Laser-Driven Microsecond Temperature Cycles Analyzed by Fluorescence Polarization Microscopy." Biophysical Journal 90, no. 8 (2006): 2958–69. http://dx.doi.org/10.1529/biophysj.105.075168.

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16

Jalocha, A., and N. F. van Hulst. "Polarization contrast in fluorescence scanning near-field optical reflection microscopy." Journal of the Optical Society of America B 12, no. 9 (1995): 1577. http://dx.doi.org/10.1364/josab.12.001577.

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17

Li, Wei, Yi Wang, Hanrong Shao, Yonghong He, and Hui Ma. "Probing rotation dynamics of biomolecules using polarization based fluorescence microscopy." Microscopy Research and Technique 70, no. 4 (2007): 390–95. http://dx.doi.org/10.1002/jemt.20418.

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18

Rao, Vishnu. "Quantifying FRET Efficiency between Fluorescent Proteins using Fluorescence Polarization Microscopy." Biophysical Journal 116, no. 3 (2019): 132a—133a. http://dx.doi.org/10.1016/j.bpj.2018.11.735.

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19

Bartle, Emily I., Siddharth Raju, and Alexa L. Mattheyses. "Protein Order in the Desmosome Investigated with Fluorescence Polarization Microscopy." Biophysical Journal 110, no. 3 (2016): 650a. http://dx.doi.org/10.1016/j.bpj.2015.11.3478.

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20

Di Battista, Diego, David Merino, Giannis Zacharakis, Pablo Loza-Alvarez, and Omar E. Olarte. "Enhanced Light Sheet Elastic Scattering Microscopy by Using a Supercontinuum Laser." Methods and Protocols 2, no. 3 (2019): 57. http://dx.doi.org/10.3390/mps2030057.

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Light sheet fluorescence microscopy techniques have revolutionized biological microscopy enabling low-phototoxic long-term 3D imaging of living samples. Although there exist many light sheet microscopy (LSM) implementations relying on fluorescence, just a few works have paid attention to the laser elastic scattering source of contrast available in every light sheet microscope. Interestingly, elastic scattering can potentially disclose valuable information from the structure and composition of the sample at different spatial scales. However, when coherent scattered light is detected with a came
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21

Qiu, Caimin, Jianling Chen, Zexian Hou, Chaoxian Xu, Shusen Xie, and Hongqin Yang. "Effect of light polariztion on pattern illumination super-resolution imaging." Journal of Innovative Optical Health Sciences 09, no. 03 (2016): 1641001. http://dx.doi.org/10.1142/s1793545816410017.

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Far-field fluorescence microscopy has made great progress in the spatial resolution, limited by light diffraction, since the super-resolution imaging technology appeared. And stimulated emission depletion (STED) microscopy and structured illumination microscopy (SIM) can be grouped into one class of the super-resolution imaging technology, which use pattern illumination strategy to circumvent the diffraction limit. We simulated the images of the beads of SIM imaging, the intensity distribution of STED excitation light and depletion light in order to observe effects of the polarized light on im
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22

Rizzo, Mark A., and David W. Piston. "High-Contrast Imaging of Fluorescent Protein FRET by Fluorescence Polarization Microscopy." Biophysical Journal 88, no. 2 (2005): L14—L16. http://dx.doi.org/10.1529/biophysj.104.055442.

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23

Buehler, Ch, C. Y. Dong, P. T. C. So, T. French, and E. Gratton. "Time-Resolved Polarization Imaging By Pump-Probe (Stimulated Emission) Fluorescence Microscopy." Biophysical Journal 79, no. 1 (2000): 536–49. http://dx.doi.org/10.1016/s0006-3495(00)76315-6.

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24

Kho, Kiang Wei, Paul R. Stoddart, Martin Harris, and Alex P. Mazzolini. "Confocal fluorescence polarization microscopy for linear unmixing of spectrally similar labels." Micron 40, no. 2 (2009): 212–17. http://dx.doi.org/10.1016/j.micron.2008.09.005.

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25

Frahm, Lars, and Jan Keller. "Polarization modulation adds little additional information to super-resolution fluorescence microscopy." Nature Methods 13, no. 1 (2015): 7–8. http://dx.doi.org/10.1038/nmeth.3687.

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26

Goto, A., T. Ohba, and K. Ohki. "Fluorescence polarization imaging microscopy by using Mueller matrix method (Part 2)." Seibutsu Butsuri 40, supplement (2000): S180. http://dx.doi.org/10.2142/biophys.40.s180_3.

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27

Artigas, David, David Merino, Christoph Polzer, and Pablo Loza-Alvarez. "Sub-diffraction discrimination with polarization-resolved two-photon excited fluorescence microscopy." Optica 4, no. 8 (2017): 911. http://dx.doi.org/10.1364/optica.4.000911.

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28

Zhang, Yaxin, Wenxia Zhou, Xiao Wang, and Jianhua Yin. "A novel reconstruction algorithm for polarization modulated fluorescence super-resolution microscopy." Optik 206 (March 2020): 163358. http://dx.doi.org/10.1016/j.ijleo.2019.163358.

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29

Xu, Dongdong, Xiao Wang, Zhibing Xu, Wenxia Zhou, and Jianhua Yin. "A super-resolution reconstruction algorithm for two-photon fluorescence polarization microscopy." Optics Communications 499 (November 2021): 127116. http://dx.doi.org/10.1016/j.optcom.2021.127116.

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30

Wazawa, Tetsuichi, Yoshiyuki Arai, Yoshinobu Kawahara, Hiroki Takauchi, Takashi Washio, and Takeharu Nagai. "Highly biocompatible super-resolution fluorescence imaging using the fast photoswitching fluorescent protein Kohinoor and SPoD-ExPAN with L p-regularized image reconstruction." Microscopy 67, no. 2 (2018): 89–98. http://dx.doi.org/10.1093/jmicro/dfy004.

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Abstract Far-field super-resolution fluorescence microscopy has enabled us to visualize live cells in great detail and with an unprecedented resolution. However, the techniques developed thus far have required high-power illumination (102–106 W/cm2), which leads to considerable phototoxicity to live cells and hampers time-lapse observation of the cells. In this study we show a highly biocompatible super-resolution microscopy technique that requires a very low-power illumination. The present technique combines a fast photoswitchable fluorescent protein, Kohinoor, with SPoD-ExPAN (super-resoluti
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31

Snell, Nicole, Vishnu Rao, Kendra Seckinger, et al. "Homotransfer FRET Reporters for Live Cell Imaging." Biosensors 8, no. 4 (2018): 89. http://dx.doi.org/10.3390/bios8040089.

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Förster resonance energy transfer (FRET) between fluorophores of the same species was recognized in the early to mid-1900s, well before modern heterotransfer applications. Recently, homotransfer FRET principles have re-emerged in biosensors that incorporate genetically encoded fluorescent proteins. Homotransfer offers distinct advantages over the standard heterotransfer FRET method, some of which are related to the use of fluorescence polarization microscopy to quantify FRET between two fluorophores of identical color. These include enhanced signal-to-noise, greater compatibility with other op
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32

Stine, Keith J., and Charles M. Knobler. "Fluorescence microscopy: A tool for studying the physical chemistry of interfaces." Proceedings, annual meeting, Electron Microscopy Society of America 49 (August 1991): 456–57. http://dx.doi.org/10.1017/s0424820100086581.

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The technique of fluorescence microscopy, a long-standing, powerful, and still evolving research tool in molecular biology, has recently found application to the study of Langmuir monolayers and to multilayers and vesicles. In this review, the basic instrumentation of fluorescence microscopy will be reviewed and a number of powerful modifications of the technique will be surveyed. Resolution limits of the techniques will be discussed. The technique of fluorescence photobleaching recovery is used to study diffusion rates. Fluorescence polarization microscopy measures orientational distributions
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33

Lidke, D. S., P. Nagy, B. G. Barisas, et al. "Imaging molecular interactions in cells by dynamic and static fluorescence anisotropy (rFLIM and emFRET)." Biochemical Society Transactions 31, no. 5 (2003): 1020–27. http://dx.doi.org/10.1042/bst0311020.

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We report the implementation and exploitation of fluorescence polarization measurements, in the form of anisotropy fluorescence lifetime imaging microscopy (rFLIM) and energy migration Förster resonance energy transfer (emFRET) modalities, for wide-field, confocal laser-scanning microscopy and flow cytometry of cells. These methods permit the assessment of rotational motion, association and proximity of cellular proteins in vivo. They are particularly applicable to probes generated by fusions of visible fluorescence proteins, as exemplified by studies of the erbB receptor tyrosine kinases invo
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34

Tang, Jialei, Jinhan Ren, and Kyu Young Han. "Fluorescence imaging with tailored light." Nanophotonics 8, no. 12 (2019): 2111–28. http://dx.doi.org/10.1515/nanoph-2019-0227.

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AbstractFluorescence microscopy has long been a valuable tool for biological and medical imaging. Control of optical parameters such as the amplitude, phase, polarization, and propagation angle of light gives fluorescence imaging great capabilities ranging from super-resolution imaging to long-term real-time observation of living organisms. In this review, we discuss current fluorescence imaging techniques in terms of the use of tailored or structured light for the sample illumination and fluorescence detection, providing a clear overview of their working principles and capabilities.
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35

Forkey, Joseph N., Margot E. Quinlan, and Yale E. Goldman. "Measurement of Single Macromolecule Orientation by Total Internal Reflection Fluorescence Polarization Microscopy." Biophysical Journal 89, no. 2 (2005): 1261–71. http://dx.doi.org/10.1529/biophysj.104.053470.

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36

Wang Xiao, 王. 潇., 赵. 远. Zhao Yuan, 杨. 凤. Yang Feng, and 尹建华 Yin Jianhua. "Fluorescence Microscopy System with High Speed Polarization Based on Electro Optical Modulator." Acta Optica Sinica 37, no. 11 (2017): 1118001. http://dx.doi.org/10.3788/aos201737.1118001.

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37

Yu, W., P. T. So, T. French, and E. Gratton. "Fluorescence generalized polarization of cell membranes: a two-photon scanning microscopy approach." Biophysical Journal 70, no. 2 (1996): 626–36. http://dx.doi.org/10.1016/s0006-3495(96)79646-7.

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38

Bigelow, Chad E., and Thomas H. Foster. "Confocal fluorescence polarization microscopy in turbid media: effects of scattering-induced depolarization." Journal of the Optical Society of America A 23, no. 11 (2006): 2932. http://dx.doi.org/10.1364/josaa.23.002932.

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39

Moh, K. J., X. C. Yuan, J. Bu, S. W. Zhu, and Bruce Z. Gao. "Radial polarization induced surface plasmon virtual probe for two-photon fluorescence microscopy." Optics Letters 34, no. 7 (2009): 971. http://dx.doi.org/10.1364/ol.34.000971.

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40

Bartle, Emily I., Tara M. Urner, Siddharth S. Raju, and Alexa L. Mattheyses. "Protein Order and Adhesive Strength in Desmosomes Determined by Fluorescence Polarization Microscopy." Biophysical Journal 112, no. 3 (2017): 299a. http://dx.doi.org/10.1016/j.bpj.2016.11.1616.

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41

Bartle, Emily I., Tara M. Urner, Siddharth S. Raju, and Alexa L. Mattheyses. "Desmoglein 3 Order and Dynamics in Desmosomes Determined by Fluorescence Polarization Microscopy." Biophysical Journal 113, no. 11 (2017): 2519–29. http://dx.doi.org/10.1016/j.bpj.2017.09.028.

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42

Fisz, Jacek J. "Fluorescence Polarization Spectroscopy at Combined High-Aperture Excitation and Detection: Application to One-Photon-Excitation Fluorescence Microscopy†." Journal of Physical Chemistry A 111, no. 35 (2007): 8606–21. http://dx.doi.org/10.1021/jp072113b.

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43

Hafi, Nour, Matthias Grunwald, Laura S. van den Heuvel, et al. "Reply to "Polarization modulation adds little additional information to super-resolution fluorescence microscopy"." Nature Methods 13, no. 1 (2015): 8–9. http://dx.doi.org/10.1038/nmeth.3721.

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44

Chen, Long, Xingye Chen, Xusan Yang, et al. "Advances of super-resolution fluorescence polarization microscopy and its applications in life sciences." Computational and Structural Biotechnology Journal 18 (2020): 2209–16. http://dx.doi.org/10.1016/j.csbj.2020.06.038.

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45

Marklund, Emil, Elias Amselem, Kalle Kipper, Magnus Johansson, Sebastian Deindl, and Johan Elf. "Measuring the Orientation of Single Proteins Interacting with DNA using Fluorescence Polarization Microscopy." Biophysical Journal 112, no. 3 (2017): 169a. http://dx.doi.org/10.1016/j.bpj.2016.11.936.

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46

Lepidi, H., Y. Zaffran, J. L. Ansaldi, J. L. Mege, and C. Capo. "Morphological polarization of human polymorphonuclear leucocytes in response to three different chemoattractants: an effector response independent of calcium rise and tyrosine kinases." Journal of Cell Science 108, no. 4 (1995): 1771–78. http://dx.doi.org/10.1242/jcs.108.4.1771.

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Chemoattractants such as interleukin-8, C5a and N-formylmethionyl-leucyl-phenylalanine induce a cytosolic calcium rise involved in triggering the secretory functions of human polymorphonuclear leucocytes. We studied the possible role of calcium rise in membrane ruffling, actin polymerization, filamentous actin distribution, and morphological polarization, which are all events contributing to chemotaxis. Membrane ruffling was assessed by right-angle light-scatter changes, the cellular content of polymerized actin by fluorescence of bodipy phallacidin, the intracellular distribution of filamento
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47

Backer, Adam S., Andreas S. Biebricher, Graeme A. King, Gijs J. L. Wuite, Iddo Heller, and Erwin J. G. Peterman. "Single-molecule polarization microscopy of DNA intercalators sheds light on the structure of S-DNA." Science Advances 5, no. 3 (2019): eaav1083. http://dx.doi.org/10.1126/sciadv.aav1083.

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DNA structural transitions facilitate genomic processes, mediate drug-DNA interactions, and inform the development of emerging DNA-based biotechnology such as programmable materials and DNA origami. While some features of DNA conformational changes are well characterized, fundamental information such as the orientations of the DNA base pairs is unknown. Here, we use concurrent fluorescence polarization imaging and DNA manipulation experiments to probe the structure of S-DNA, an elusive, elongated conformation that can be accessed by mechanical overstretching. To this end, we directly quantify
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48

Levenson, Richard. "Spectral Imaging: Fluorescence and Brightfield." Microscopy and Microanalysis 7, S2 (2001): 20–21. http://dx.doi.org/10.1017/s1431927600026179.

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Spectral imaging is a relatively new technique that provides images of a scene at multiple wavelengths and can generate precise optical spectra at every pixel. Mathematical approaches may then be used to extract the maximum information from the spectral image. Spectral imaging is routinely used in “remote sensing”, that is, the analysis of distant landscapes and structures from airplanes or satellites. Minor differences in spectra can be used to detect different crops, or mineral deposits, for example. Closer in, spectral imaging has uses in industrial process control, detection of otherwise i
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49

Campagnola, P. J., and L. M. Loew. "Second Harmonic Generation Imaging (SHG) in the Non-Linear Optical Microscopy of Living Cells." Microscopy and Microanalysis 4, S2 (1998): 414–15. http://dx.doi.org/10.1017/s1431927600022194.

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In recent years there has been considerable interest in two and three-photon excited fluorescence in laser scanning optical microscopy. Because absorption is confined tot he focal plane of the objective, these techniques provide intrinsic optical sectioning without the use of a confocal aperture. In addition, photobleaching and phototoxicity are greatly reduced above and below the focal plane. We have adapted a two-photon microscope to utilize surface second harmonic generation (SHG) as a new contrast mechanism for nonlinear optical biological imaging.Surface SHG was first described by Shen [1
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50

Parasassi, T., E. Gratton, W. M. Yu, P. Wilson, and M. Levi. "Two-photon fluorescence microscopy of laurdan generalized polarization domains in model and natural membranes." Biophysical Journal 72, no. 6 (1997): 2413–29. http://dx.doi.org/10.1016/s0006-3495(97)78887-8.

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