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Journal articles on the topic 'Fluorescence spectroscopy. Nanotechnology. Membranes (Biology)'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Xu, Zhi, Wen-Min Su, and George M. Carman. "Fluorescence spectroscopy measures yeastPAH1-encoded phosphatidate phosphatase interaction with liposome membranes." Journal of Lipid Research 53, no. 3 (2011): 522–28. http://dx.doi.org/10.1194/jlr.m022798.

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Kahya, Nicoletta, Dag Scherfeld, Kirsten Bacia, Bert Poolman, and Petra Schwille. "Probing Lipid Mobility of Raft-exhibiting Model Membranes by Fluorescence Correlation Spectroscopy." Journal of Biological Chemistry 278, no. 30 (2003): 28109–15. http://dx.doi.org/10.1074/jbc.m302969200.

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3

Palmer, Arthur G., and Nancy L. Thompson. "Fluorescence correlation spectroscopy for detecting submicroscopic clusters of fluorescent molecules in membranes." Chemistry and Physics of Lipids 50, no. 3-4 (1989): 253–70. http://dx.doi.org/10.1016/0009-3084(89)90053-4.

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4

Thompson, Nancy L., Xiang Wang, and Punya Navaratnarajah. "Total internal reflection with fluorescence correlation spectroscopy: Applications to substrate-supported planar membranes." Journal of Structural Biology 168, no. 1 (2009): 95–106. http://dx.doi.org/10.1016/j.jsb.2009.02.013.

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5

Schwille, Petra, Jonas Korlach, and Watt W. Webb. "Fluorescence correlation spectroscopy with single-molecule sensitivity on cell and model membranes." Cytometry 36, no. 3 (1999): 176–82. http://dx.doi.org/10.1002/(sici)1097-0320(19990701)36:3<176::aid-cyto5>3.0.co;2-f.

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Sinner, Eva-Kathrin, Ute Reuning, Fatma Nese Kök, et al. "Incorporation of integrins into artificial planar lipid membranes: characterization by plasmon-enhanced fluorescence spectroscopy." Analytical Biochemistry 333, no. 2 (2004): 216–24. http://dx.doi.org/10.1016/j.ab.2004.05.022.

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Pramanik, Aladdin, Per Thyberg, and Rudolf Rigler. "Molecular interactions of peptides with phospholipid vesicle membranes as studied by fluorescence correlation spectroscopy." Chemistry and Physics of Lipids 104, no. 1 (2000): 35–47. http://dx.doi.org/10.1016/s0009-3084(99)00113-9.

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8

Cabré, Elisa J., Luís M. S. Lourab, Bárbara Olmeda, Alexander Fedorov, Jesus Pérez-Gil, and Manuel Prieto. "Structural characterization of pulmonary surfactant protein SP-B in model membranes by fluorescence spectroscopy." Chemistry and Physics of Lipids 149 (September 2007): S12—S13. http://dx.doi.org/10.1016/j.chemphyslip.2007.06.027.

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Bag, Nirmalya, David A. Holowka, and Barbara A. Baird. "Imaging FCS delineates subtle heterogeneity in plasma membranes of resting mast cells." Molecular Biology of the Cell 31, no. 7 (2020): 709–23. http://dx.doi.org/10.1091/mbc.e19-10-0559.

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The plasma membrane’s resting organization must be poised to respond efficiently to external stimuli. Analysis of very large data sets from imaging fluorescence correlation spectroscopy parameterizes diffusion properties from structurally distinct probes to provide a composite picture of subtle interactions underlying poised membrane heterogeneity.

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Korlach, Jonas, Tobias Baumgart, Watt W. Webb, and Gerald W. Feigenson. "Detection of motional heterogeneities in lipid bilayer membranes by dual probe fluorescence correlation spectroscopy." Biochimica et Biophysica Acta (BBA) - Biomembranes 1668, no. 2 (2005): 158–63. http://dx.doi.org/10.1016/j.bbamem.2004.11.016.

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Lambrev, Petar H., and Parveen Akhtar. "Macroorganisation and flexibility of thylakoid membranes." Biochemical Journal 476, no. 20 (2019): 2981–3018. http://dx.doi.org/10.1042/bcj20190080.

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Abstract The light reactions of photosynthesis are hosted and regulated by the chloroplast thylakoid membrane (TM) — the central structural component of the photosynthetic apparatus of plants and algae. The two-dimensional and three-dimensional arrangement of the lipid–protein assemblies, aka macroorganisation, and its dynamic responses to the fluctuating physiological environment, aka flexibility, are the subject of this review. An emphasis is given on the information obtainable by spectroscopic approaches, especially circular dichroism (CD). We briefly summarise the current knowledge of the

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Macháň, Radek, and Martin Hof. "Recent Developments in Fluorescence Correlation Spectroscopy for Diffusion Measurements in Planar Lipid Membranes." International Journal of Molecular Sciences 11, no. 2 (2010): 427–57. http://dx.doi.org/10.3390/ijms11020427.

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Kużdżał, Michał, Olga Wesołowska, Janez Štrancar, and Krystyna Michalak. "Fluorescence and ESR spectroscopy studies on the interaction of isoflavone genistein with biological and model membranes." Chemistry and Physics of Lipids 164, no. 4 (2011): 283–91. http://dx.doi.org/10.1016/j.chemphyslip.2011.03.001.

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Vogel, Hans J., David J. Schibli, Weiguo Jing, Elke M. Lohmeier-Vogel, Raquel F. Epand, and Richard M. Epand. "Towards a structure-function analysis of bovine lactoferricin and related tryptophan- and arginine-containing peptides." Biochemistry and Cell Biology 80, no. 1 (2002): 49–63. http://dx.doi.org/10.1139/o01-213.

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The iron-binding protein lactoferrin is a multifunctional protein that has antibacterial, antifungal, antiviral, antitumour, anti-inflammatory, and immunoregulatory properties. All of these additional properties appear to be related to its highly basic N-terminal region. This part of the protein can be released in the stomach by pepsin cleavage at acid pH. The 25-residue antimicrobial peptide that is released is called lactoferricin. In this work, we review our knowledge about the structure of the peptide and attempt to relate this to its many functions. Microcalorimetry and fluorescence spect

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15

Bag, Nirmalya, Darilyn Hui Xin Yap, and Thorsten Wohland. "Temperature dependence of diffusion in model and live cell membranes characterized by imaging fluorescence correlation spectroscopy." Biochimica et Biophysica Acta (BBA) - Biomembranes 1838, no. 3 (2014): 802–13. http://dx.doi.org/10.1016/j.bbamem.2013.10.009.

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16

Tauc, Patrick, C. Reyes Mateo, and Jean-Claude Brochon. "Investigation of the effect of high hydrostatic pressure on proteins and lipidic membranes by dynamic fluorescence spectroscopy." Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology 1595, no. 1-2 (2002): 103–15. http://dx.doi.org/10.1016/s0167-4838(01)00338-7.

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17

Markovic, Dejan Z., and Robert Carpentier. "Relationship between quenching of variable fluorescence and thermal dissipation in isolated thylakoid membranes: similar terminology and mathematical treatments may be used." Biochemistry and Cell Biology 73, no. 5-6 (1995): 247–52. http://dx.doi.org/10.1139/o95-030.

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Simultaneous measurements of chlorophyll fluorescence and thermal emission using photoacoustic spectroscopy have been done in isolated thylakoid membranes to study the relationship between the photochemical quenching of fluorescence (qPF) and energy storage measured in photoacoustic experiments. It is shown that energy storage can be interpreted as the photochemical quenching of a variable component of thermal dissipation termed qPH. The parameters qPF and qPH were similarly sensitive to light intensity as demonstrated by their half-saturation light intensity. However, the nonvariable part of

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Pramanik, A., A. Juréus, Ü. Langel, T. Bartfai, and R. Rigler. "Galanin receptor binding studies in the membranes of cultured cells measured by fluorescence correlation spectroscopy." Biomedical Chromatography 13, no. 2 (1999): 119–20. http://dx.doi.org/10.1002/(sici)1099-0801(199904)13:2<119::aid-bmc835>3.0.co;2-p.

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Jobin, Marie-Lise, Lydie Vamparys, Romain Deniau, et al. "Biophysical Insight on the Membrane Insertion of an Arginine-Rich Cell-Penetrating Peptide." International Journal of Molecular Sciences 20, no. 18 (2019): 4441. http://dx.doi.org/10.3390/ijms20184441.

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Cell-penetrating peptides (CPPs) are short peptides that can translocate and transport cargoes into the intracellular milieu by crossing biological membranes. The mode of interaction and internalization of cell-penetrating peptides has long been controversial. While their interaction with anionic membranes is quite well understood, the insertion and behavior of CPPs in zwitterionic membranes, a major lipid component of eukaryotic cell membranes, is poorly studied. Herein, we investigated the membrane insertion of RW16 into zwitterionic membranes, a versatile CPP that also presents antibacteria

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Crepin, Aurélie, Edel Cunill-Semanat, Eliška Kuthanová Trsková, Erica Belgio, and Radek Kaňa. "Antenna Protein Clustering In Vitro Unveiled by Fluorescence Correlation Spectroscopy." International Journal of Molecular Sciences 22, no. 6 (2021): 2969. http://dx.doi.org/10.3390/ijms22062969.

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Antenna protein aggregation is one of the principal mechanisms considered effective in protecting phototrophs against high light damage. Commonly, it is induced, in vitro, by decreasing detergent concentration and pH of a solution of purified antennas; the resulting reduction in fluorescence emission is considered to be representative of non-photochemical quenching in vivo. However, little is known about the actual size and organization of antenna particles formed by this means, and hence the physiological relevance of this experimental approach is questionable. Here, a quasi-single molecule m

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Tietz, Stefanie, Michelle Leuenberger, Ricarda Höhner, Alice H. Olson, Graham R. Fleming, and Helmut Kirchhoff. "A proteoliposome-based system reveals how lipids control photosynthetic light harvesting." Journal of Biological Chemistry 295, no. 7 (2020): 1857–66. http://dx.doi.org/10.1074/jbc.ra119.011707.

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Integral membrane proteins are exposed to a complex and dynamic lipid environment modulated by nonbilayer lipids that can influence protein functions by lipid-protein interactions. The nonbilayer lipid monogalactosyldiacylglycerol (MGDG) is the most abundant lipid in plant photosynthetic thylakoid membranes, but its impact on the functionality of energy-converting membrane protein complexes is unknown. Here, we optimized a detergent-based reconstitution protocol to develop a proteoliposome technique that incorporates the major light-harvesting complex II (LHCII) into compositionally well-defin

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Buskaran, Kalaivani, Mohd Zobir Hussein, Mohamad Aris Mohd Moklas, Mas Jaffri Masarudin, and Sharida Fakurazi. "Graphene Oxide Loaded with Protocatechuic Acid and Chlorogenic Acid Dual Drug Nanodelivery System for Human Hepatocellular Carcinoma Therapeutic Application." International Journal of Molecular Sciences 22, no. 11 (2021): 5786. http://dx.doi.org/10.3390/ijms22115786.

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Hepatocellular carcinoma or hepatoma is a primary malignant neoplasm that responsible for 75–90% of all liver cancer in humans. Nanotechnology introduced the dual drug nanodelivery method as one of the initiatives in nanomedicine for cancer therapy. Graphene oxide (GO) loaded with protocatechuic acid (PCA) and chlorogenic acid (CA) have shown some anticancer activities in both passive and active targeting. The physicochemical characterizations for nanocomposites were conducted. Cell cytotoxicity assay and lactate dehydrogenase were conducted to estimate cell cytotoxicity and the severity of ce

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23

Kleusch, Christian, Cornelia Monzel, Krishna Chander Sridhar, Bernd Hoffmann, Agnes Csiszár, and Rudolf Merkel. "Fluorescence Correlation Spectroscopy Reveals Interaction of Some Microdomain-Associated Lipids with Cellular Focal Adhesion Sites." International Journal of Molecular Sciences 21, no. 21 (2020): 8149. http://dx.doi.org/10.3390/ijms21218149.

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Cells adhere to the extracellular matrix at distinct anchoring points, mostly focal adhesions. These are rich in immobile transmembrane- and cytoskeletal-associated proteins, some of which are known to interact with lipids of the plasma membrane. To investigate their effect on lipid mobility and molecular interactions, fluorescently labeled lipids were incorporated into the plasma membranes of primary myofibroblasts using fusogenic liposomes. With fluorescence correlation spectroscopy, we tested mobilities of labeled microdomain-associated lipids such as sphingomyelin (SM), ganglioside (GM1),

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Suwalsky, Mario, Carlos Schneider, Beryl Norris, et al. "The Local Anesthetic Proparacaine Modifies Sodium Transport in Toad Skin and Perturbs the Structures of Model and Cell Membranes." Zeitschrift für Naturforschung C 57, no. 9-10 (2002): 930–38. http://dx.doi.org/10.1515/znc-2002-9-1029.

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Experimental results indicate a significant decrease in the potential difference (PD) and in the short-circuit current (Isc) after the application of proparacaine to isolated toad skin, which may reflect an inhibition of the active transport of ions. This finding was explained on the basis of the results obtained from membrane models incubated with proparacaine. These consisted of human erythrocytes, isolated unsealed human erythrocyte membranes (IUM), phospholipid multilayers built-up of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), representatives of p

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Belous, A. M., V. I. Lugovoj, V. A. Moiseyev, O. A. Nardid, and V. I. Zagnoyko. "Examination by fluorescence and EPR spectroscopy of the state of mitochondrial and lysosomal membranes upon freeze-thawing." Cryobiology 27, no. 2 (1990): 184–88. http://dx.doi.org/10.1016/0011-2240(90)90010-2.

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Vámosi, György, Elza Friedländer-Brock, Shehu M. Ibrahim, Roland Brock, János Szöllősi, and György Vereb. "EGF Receptor Stalls upon Activation as Evidenced by Complementary Fluorescence Correlation Spectroscopy and Fluorescence Recovery after Photobleaching Measurements." International Journal of Molecular Sciences 20, no. 13 (2019): 3370. http://dx.doi.org/10.3390/ijms20133370.

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To elucidate the molecular details of the activation-associated clustering of epidermal growth factor receptors (EGFRs), the time course of the mobility and aggregation states of eGFP tagged EGFR in the membranes of Chinese hamster ovary (CHO) cells was assessed by in situ mobility assays. Fluorescence correlation spectroscopy (FCS) was used to probe molecular movements of small ensembles of molecules over short distances and time scales, and to report on the state of aggregation. The diffusion of larger ensembles of molecules over longer distances (and time scales) was investigated by fluores

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Verhagen, J. C., P. ter Braake, J. Teunissen, G. van Ginkel, and A. Sevanian. "Physical effects of biologically formed cholesterol oxidation products on lipid membranes investigated with fluorescence depolarization spectroscopy and electron spin resonance." Journal of Lipid Research 37, no. 7 (1996): 1488–502. http://dx.doi.org/10.1016/s0022-2275(20)39133-1.

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Murali, J., D. Koteeswari, J. M. Rifkind, and R. Jayakumar. "Amyloid insulin interaction with erythrocytes." Biochemistry and Cell Biology 81, no. 1 (2003): 51–59. http://dx.doi.org/10.1139/o03-009.

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Erythrocyte membrane interactions with insulin fibrils (amyloid) have been investigated using centrifugation, fluorescence spectroscopy, light scattering, and flow cytometric techniques. The results indicate that insulin fibrils are having moderate affinity to erythrocyte membrane. However, analysis of the apparent dissociation constants of human erythrocyte membranes (leaky and resealed vesicles) with amyloid insulin reveal that the insulin binding is drastically reduced on attaining the fibrillar state compared with native insulin. To understand the role of insulin receptors on erythrocytes

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Bonanno, Alexander, Robert C. Blake, and Parkson Lee-Gau Chong. "Sulfolobus acidocaldarius Microvesicles Exhibit Unusually Tight Packing Properties as Revealed by Optical Spectroscopy." International Journal of Molecular Sciences 20, no. 21 (2019): 5308. http://dx.doi.org/10.3390/ijms20215308.

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In this study, we used optical spectroscopy to characterize the physical properties of microvesicles released from the thermoacidophilic archaeon Sulfolobus acidocaldarius (Sa-MVs). The most abundant proteins in Sa-MVs are the S-layer proteins, which self-assemble on the vesicle surface forming an array of crystalline structures. Lipids in Sa-MVs are exclusively bipolar tetraethers. We found that when excited at 275 nm, intrinsic protein fluorescence of Sa-MVs at 23 °C has an emission maximum at 303 nm (or 296 nm measured at 75 °C), which is unusually low for protein samples containing multipl

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Jing, Weiguo, Alistair R. Demcoe, and Hans J. Vogel. "Conformation of a Bactericidal Domain of Puroindoline a: Structure and Mechanism of Action of a 13-Residue Antimicrobial Peptide." Journal of Bacteriology 185, no. 16 (2003): 4938–47. http://dx.doi.org/10.1128/jb.185.16.4938-4947.2003.

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ABSTRACT Puroindoline a, a wheat endosperm-specific protein containing a tryptophan-rich domain, was reported to have antimicrobial activities. We found that a 13-residue fragment of puroindoline a (FPVTWRWWKWWKG-NH2) (puroA) exhibits activity against both gram-positive and gram-negative bacteria. This suggests that puroA may be a bactericidal domain of puroindoline a. PuroA interacted strongly with negatively charged phospholipid vesicles and induced efficient dye release from these vesicles, suggesting that the microbicidal effect of puroA may be due to interactions with bacterial membranes.

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Sarkar, D. P., S. J. Morris, O. Eidelman, J. Zimmerberg, and R. Blumenthal. "Initial stages of influenza hemagglutinin-induced cell fusion monitored simultaneously by two fluorescent events: cytoplasmic continuity and lipid mixing." Journal of Cell Biology 109, no. 1 (1989): 113–22. http://dx.doi.org/10.1083/jcb.109.1.113.

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We have monitored the mixing of both aqueous intracellular and membrane-bound fluorescent dyes during the fusion of human red blood cells to influenza hemagglutinin-expressing fibroblasts using fluorescence spectroscopy and low light, image-enhanced video microscopy. The water-soluble fluorescent dye, N-(7-nitrobenzofurazan-4-yl)taurine, was incorporated into intact human red blood cells. The fluorescence of the dye in the intact red blood cell was partially quenched by hemoglobin. The lipid fluorophore, octadecylrhodamine, was incorporated into the membrane of the same red blood cell at self-

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Suwalsky, M., F. Villena, F. Aguilar, and C. P. Sotomayor. "Interaction of Penicillin G with the Human Erythrocyte Membrane and Models." Zeitschrift für Naturforschung C 51, no. 3-4 (1996): 243–48. http://dx.doi.org/10.1515/znc-1996-3-416.

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Abstract Penicillin G (PEN) is a widely used antibiotic whose mechanism of action is related to the interference with the synthesis of bacteria cell wall. In order to evaluate its perturbing effect upon human cell membranes PEN was made to interact with human erythrocytes, isolated resealed human erythrocyte membranes and molecular models. The latter were multibilayers of the phospholipids dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidyl-ethanolamine (DMPE) as well as DMPC large unilamellar vesicles. These studies were per formed by scanning electron microscopy, fluorescence

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Tukova, Anastasiia, and Alison Rodger. "Spectroscopy of model-membrane liposome-protein systems: complementarity of linear dichroism, circular dichroism, fluorescence and SERS." Emerging Topics in Life Sciences 5, no. 1 (2021): 61–75. http://dx.doi.org/10.1042/etls20200354.

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A range of membrane models have been developed to study components of cellular systems. Lipid vesicles or liposomes are one such artificial membrane model which mimics many properties of the biological system: they are lipid bilayers composed of one or more lipids to which other molecules can associate. Liposomes are thus ideal to study the roles of cellular lipids and their interactions with other membrane components to understand a wide range of cellular processes including membrane disruption, membrane transport and catalytic activity. Although liposomes are much simpler than cellular membr

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Sugár, István, Alexander Bonanno, and Parkson Chong. "Gramicidin Lateral Distribution in Phospholipid Membranes: Fluorescence Phasor Plots and Statistical Mechanical Model." International Journal of Molecular Sciences 19, no. 11 (2018): 3690. http://dx.doi.org/10.3390/ijms19113690.

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When using small mole fraction increments to study gramicidins in phospholipid membranes, we found that the phasor dots of intrinsic fluorescence of gramicidin D and gramicidin A in dimyristoyl-sn-glycero-3-phosphocholine (DMPC) unilamellar and multilamellar vesicles exhibit a biphasic change with peptide content at 0.143 gramicidin mole fraction. To understand this phenomenon, we developed a statistical mechanical model of gramicidin/DMPC mixtures. Our model assumes a sludge-like mixture of fluid phase and aggregates of rigid clusters. In the fluid phase, gramicidin monomers are randomly dist

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Henriques, Sónia Troeira, Evelyne Deplazes, Nicole Lawrence, et al. "Interaction of Tarantula Venom Peptide ProTx-II with Lipid Membranes Is a Prerequisite for Its Inhibition of Human Voltage-gated Sodium Channel NaV1.7." Journal of Biological Chemistry 291, no. 33 (2016): 17049–65. http://dx.doi.org/10.1074/jbc.m116.729095.

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ProTx-II is a disulfide-rich peptide toxin from tarantula venom able to inhibit the human voltage-gated sodium channel 1.7 (hNaV1.7), a channel reported to be involved in nociception, and thus it might have potential as a pain therapeutic. ProTx-II acts by binding to the membrane-embedded voltage sensor domain of hNaV1.7, but the precise peptide channel-binding site and the importance of membrane binding on the inhibitory activity of ProTx-II remain unknown. In this study, we examined the structure and membrane-binding properties of ProTx-II and several analogues using NMR spectroscopy, surfac

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Scott, Angela M., Corina E. Antal та Alexandra C. Newton. "Electrostatic and Hydrophobic Interactions Differentially Tune Membrane Binding Kinetics of the C2 Domain of Protein Kinase Cα". Journal of Biological Chemistry 288, № 23 (2013): 16905–15. http://dx.doi.org/10.1074/jbc.m113.467456.

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The cellular activation of conventional protein kinase C (PKC) isozymes is initiated by the binding of their C2 domains to membranes in response to elevations in intracellular Ca2+. Following this C2 domain-mediated membrane recruitment, the C1 domain binds its membrane-embedded ligand diacylglycerol, resulting in activation of PKC. Here we explore the molecular mechanisms by which the C2 domain controls the initial step in the activation of PKC. Using stopped-flow fluorescence spectroscopy to measure association and dissociation rate constants, we show that hydrophobic interactions are the ma

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Vogel, Alexander, Jörg Nikolaus, Katrin Weise, et al. "Interaction of the human N-Ras protein with lipid raft model membranes of varying degrees of complexity." Biological Chemistry 395, no. 7-8 (2014): 779–89. http://dx.doi.org/10.1515/hsz-2013-0294.

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Abstract Ternary lipid mixtures composed of cholesterol, saturated (frequently with sphingosine backbone), and unsaturated phospholipids show stable phase separation and are often used as model systems of lipid rafts. Yet, their ability to reproduce raft properties and function is still debated. We investigated the properties and functional aspects of three lipid raft model systems of varying degrees of biological relevance – PSM/POPC/Chol, DPPC/POPC/Chol, and DPPC/DOPC/Chol – using 2H solid-state nuclear magnetic resonance (NMR) spectroscopy, fluorescence microscopy, and atomic force microsco

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Suwalsky, M., M. Benites, F. Villena, F. Aguilar, and C. P. Sotomayor. "Interaction of the Organochlorine Pesticide Dieldrin with Phospholipid Bilayers." Zeitschrift für Naturforschung C 52, no. 7-8 (1997): 450–58. http://dx.doi.org/10.1515/znc-1997-7-806.

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Abstract Dieldrin is an organochlorine insecticide highly toxic for human beings. Although its exact mechanism of action is not well known, there is evidence that it acts at the cell membrane level. In fact, the lipophilicity of the pesticide as well as that of the phospholipid bilayer present in biological membranes makes the latter a most likely target for the interaction of dieldrin with living organisms. In order to evaluate its perturbing effect upon cell membranes the pesticide was made to interact with human erythrocytes and molecular models. These studies were performed by scanning ele

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Suwara, Justyna, Beata Lukasik, Remigiusz Zurawinski, Roza Pawlowska, and Arkadiusz Chworos. "Highly Fluorescent Distyrylnaphthalene Derivatives as a Tool for Visualization of Cellular Membranes." Materials 13, no. 4 (2020): 951. http://dx.doi.org/10.3390/ma13040951.

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Fluorescent imaging, which is an important interdisciplinary field bridging research from organic chemistry, biochemistry and cell biology has been applied for multi-dimensional detection, visualization and characterization of biological structures and processes. Especially valuable is the possibility to monitor cellular processes in real time using fluorescent probes. In this work, conjugated oligoelectrolytes and neutral derivatives with the distyrylnaphthalene core (SN-COEs) were designed, synthetized and tested for biological properties as membrane-specific fluorescent dyes for the visuali

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Ripanti, Francesca, Almerinda Di Venere, Mariangela Cestelli Guidi, et al. "The Puzzling Problem of Cardiolipin Membrane-Cytochrome c Interactions: A Combined Infrared and Fluorescence Study." International Journal of Molecular Sciences 22, no. 3 (2021): 1334. http://dx.doi.org/10.3390/ijms22031334.

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The interaction of cytochrome c (cyt c) with natural and synthetic membranes is known to be a complex phenomenon, involving both protein and lipid conformational changes. In this paper, we combined infrared and fluorescence spectroscopy to study the structural transformation occurring to the lipid network of cardiolipin-containing large unilamellar vesicles (LUVs). The data, collected at increasing protein/lipid ratio, demonstrate the existence of a multi-phase process, which is characterized by: (i) the interaction of cyt c with the lipid polar heads; (ii) the lipid anchorage of the protein o

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Charbonneau, Sophie, Henry Peng, Pang N. Shek, and Mark Blostein. "Use of Amphipathic Helical Peptides as An Anticoagulant." Blood 112, no. 11 (2008): 4100. http://dx.doi.org/10.1182/blood.v112.11.4100.4100.

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Abstract We have previously shown that an ideal amphipathic helical peptide of K7L15 composition (IAP) accelerates factor IXa-mediated factor X turnover and factor Xamediated prothrombin turnover in a phospholipid free system (Biochem J., 2008, 412:545). Under these conditions, IAP behaves as a phospholipid membrane allowing coagulation factors to bind and exert their actions. However, when IAP is used with in vitro assays that employ phospholipids such as an active partial thromboplastin time (aPTT), IAP paradoxically behaves as an anticoagulant by prolonging clotting times. We hypothesize th

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Yu, Lanlan, Jeak Ling Ding, Bow Ho, and Thorsten Wohland. "Investigation of a novel artificial antimicrobial peptide by fluorescence correlation spectroscopy: An amphipathic cationic pattern is sufficient for selective binding to bacterial type membranes and antimicrobial activity." Biochimica et Biophysica Acta (BBA) - Biomembranes 1716, no. 1 (2005): 29–39. http://dx.doi.org/10.1016/j.bbamem.2005.08.005.

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Williams, Thomas L., Benjamin R. G. Johnson, Brigita Urbanc, A. Toby A. Jenkins, Simon D. A. Connell та Louise C. Serpell. "Aβ42 oligomers, but not fibrils, simultaneously bind to and cause damage to ganglioside-containing lipid membranes". Biochemical Journal 439, № 1 (2011): 67–77. http://dx.doi.org/10.1042/bj20110750.

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Aβ (amyloid-β peptide) assembles to form amyloid fibres that accumulate in senile plaques associated with AD (Alzheimer's disease). The major constituent, a 42-residue Aβ, has the propensity to assemble and form soluble and potentially cytotoxic oligomers, as well as ordered stable amyloid fibres. It is widely believed that the cytotoxicity is a result of the formation of transient soluble oligomers. This observed toxicity may be associated with the ability of oligomers to associate with and cause permeation of lipid membranes. In the present study, we have investigated the ability of oligomer

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Tkachenko, Anton, Anatolii Onishchenko, Vladimir Klochkov, et al. "The impact of orally administered gadolinium orthovanadate GdVO4:Eu3+ nanoparticles on the state of phospholipid bilayer of erythrocytes." Turkish Journal of Biochemistry 45, no. 4 (2020): 389–95. http://dx.doi.org/10.1515/tjb-2019-0427.

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AbstractObjectivesTo assess the state of phospholipid bilayer of red blood cells (RBCs) in rats orally exposed to gadolinium orthovanadate GdVO4:Eu3+ nanoparticles (VNPs) during two weeks using fluorescent probes − ortho-hydroxy derivatives of 2,5-diaryl-1,3-oxazole.MethodsSteady-state fluorescence spectroscopy: a study by the environment-sensitive fluorescent probes − 2-(2′-OH-phenyl)-5-phenyl-1,3-oxazole (probe O1O) and 2-(2′-OH-phenyl)-phenanthro[9,10]-1,3-oxazole (probe PH7).ResultsNo significant changes are detected in the spectra of the fluorescent probes bound to the RBCs from the rats

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Graf, Josef A., Karin Witzan, and Reto J. Strasser. "Cerulenin-Induced Modifications in the Fatty Acid Composition Affect Excitation Energy Transfer in Thylakoids of Petunia hybrida Leaves." Zeitschrift für Naturforschung C 43, no. 5-6 (1988): 431–37. http://dx.doi.org/10.1515/znc-1988-5-618.

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Cerulenin-induced modifications in the fatty acid composition have been used to investigate the influence of acyl lipids on excitation energy distribution in thylakoid membranes of Petunia hybrida by means of 77 K fluorescence spectroscopy. Although cerulenin has no effect on relative contents of chlorophyll and acyl lipids, changes in the fatty acid composition of all thylakoid acyl lipids have been observed. The main cerulenin effect seems to be an increase in linoleic acid at the expense of saturated and monounsaturated C16- and C18-fatty acids resulting most likely in an increase in acyl l

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Zimmermann, Ralf, David Küttner, Lars Renner, et al. "Charging and structure of zwitterionic supported bilayer lipid membranes studied by streaming current measurements, fluorescence microscopy, and attenuated total reflection Fourier transform infrared spectroscopy." Biointerphases 4, no. 1 (2009): 1–6. http://dx.doi.org/10.1116/1.3082042.

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Suwalsky, Mario, Carlos Schneider, Fernando Villena, et al. "Dibucaine-Induced Modification of Sodium Transport in Toad Skin and of Model Membrane Structures." Zeitschrift für Naturforschung C 56, no. 7-8 (2001): 614–22. http://dx.doi.org/10.1515/znc-2001-7-822.

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The interaction of the local anesthetic dibucaine with the isolated toad skin and membrane models is described. The latter consisted of human erythrocytes, isolated unsealed human erythrocyte membranes (IUM), large unilamellar vesicles (LUV) of dimyristoylphosphati-dylcholine (DMPC) and phospholipid multilayers built-up of DMPC and dimyristoylphos-phatidylethanolamine (DMPE), representative of phospholipid classes located in the outer and inner monolayers of the human erythrocyte membrane, respectively. Results indicate a significant decrease in the potential difference (PD) and in the short-c

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Killi, Dilek, Antonio Raschi, and Filippo Bussotti. "Lipid Peroxidation and Chlorophyll Fluorescence of Photosystem II Performance during Drought and Heat Stress is Associated with the Antioxidant Capacities of C3 Sunflower and C4 Maize Varieties." International Journal of Molecular Sciences 21, no. 14 (2020): 4846. http://dx.doi.org/10.3390/ijms21144846.

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Agricultural production is predicted to be adversely affected by an increase in drought and heatwaves. Drought and heat damage cellular membranes, such as the thylakoid membranes where photosystem II occurs (PSII). We investigated the chlorophyll fluorescence (ChlF) of PSII, photosynthetic pigments, membrane damage, and the activity of protective antioxidants in drought-tolerant and -sensitive varieties of C3 sunflower and C4 maize grown at 20/25 and 30/35 °C. Drought-tolerant varieties retained PSII electron transport at lower levels of water availability at both temperatures. Drought and hea

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Schneckenburger, Herbert, Petra Weber, Michael Wagner, et al. "Combining TIR and FRET in Molecular Test Systems." International Journal of Molecular Sciences 20, no. 3 (2019): 648. http://dx.doi.org/10.3390/ijms20030648.

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Pharmaceutical agents or drugs often have a pronounced impact on protein-protein interactions in cells, and in particular, cell membranes. Changes of molecular conformations as well as of intermolecular interactions may affect dipole-dipole interaction between chromophoric groups, which can be proven by measuring the Förster resonance energy transfer (FRET). If these chromophores are located within or in close proximity to the plasma membrane, they are excited preferentially by an evanescent electromagnetic wave upon total internal reflection (TIR) of an incident laser beam. For the TIR-FRET s

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Húmpola, María Verónica, María Carolina Rey, Pablo Gabriel Spontón, Arturo Carlos Simonetta, and Georgina Guadalupe Tonarelli. "A Comparative Study of the Antimicrobial and Structural Properties of Short Peptides and Lipopeptides Containing a Repetitive Motif KLFK." Protein & Peptide Letters 26, no. 3 (2019): 192–203. http://dx.doi.org/10.2174/0929866526666181208144629.

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Background:In the last years, Antimicrobial Peptides (AMPs) and lipopeptides have received attention as promising candidates to treat infections caused by resistant microorganisms. </P><P> Objective: The main objective of this study was to investigate the effect of repetitive KLFK motifs and the attachment of aliphatic acids to the N-terminus of (KLFK)n peptides on therapeutic properties.Methods:Minimal inhibitory concentration against Gram (+) and (-) bacteria and yeast of synthetic compounds were determined by broth microtiter dilution method, and the toxicity was evaluated by he

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