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Academic literature on the topic 'Fluorescens'

Author: Grafiati

Published: 4 June 2021

Last updated: 6 September 2023

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Journal articles on the topic "Fluorescens"

1

Etebarian, Hassan-Reza, Peter L. Sholberg, Kenneth C. Eastwell, and Ronald J. Sayler. "Biological control of apple blue mold withPseudomonas fluorescens." Canadian Journal of Microbiology 51, no. 7 (July 1, 2005): 591–98. http://dx.doi.org/10.1139/w05-039.

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Pseudomonas fluorescens isolate 1100-6 was evaluated as a potential biological control agent for apple blue mold caused by Penicillium expansum or Penicillium solitum. Both the wild-type isolate 1100-6 and a genetically modified derivative labeled with the gene encoding the green fluorescent protein (GFP) were compared. The P. fluorescens isolates with or without GFP equally reduced the growth of Penicillium spp. and produced large zones of inhibition in dual culture plate assays. Cell-free metabolites produced by the bacterial antagonists reduced the colony area of Penicillium isolates by 17.3% to 78.5%. The effect of iron chelate on the antagonistic potential of P. fluorescens was also studied. The use of iron chelate did not have a major effect on the antagonistic activity of P. fluorescens. With or without GFP, P. fluorescens significantly reduced the severity and incidence of apple decay by 2 P. expansum isolates after 11 d at 20 °C and by P. expansum and P. solitum after 25 d at 5 °C when the biocontrol agents were applied in wounds 24 or 48 h before challenging with Penicillium spp. Populations of P. fluorescens labeled with the GFP were determined 1, 9, 14, and 20 d after inoculation at 5 °C. The log CFU/mL per wound increased from 6.95 at the time of inoculation to 9.12 CFU/mL (P < 0.05) 25 d after inoculation at 5 °C. The GFP strain did not appear to penetrate deeply into wounds based on digital photographs taken with an inverted fluorescence microscope. These results indicate that P. fluorescens isolate 1100-6 could be an important new biological control for apple blue mold.Key words: Penicillium expansum, P. solitum, postharvest disease, Malus, GFP.

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Gopal, Surendra, Reshma Francis, and A. K. Sreelatha. "Impact of soil temperature, pH and carbon dioxide on the population and efficiency of fluorescent pseudomonad in the rhizosphere soil of Pokkali rice." Environment Conservation Journal 24, no. 1 (January 8, 2023): 163–70. http://dx.doi.org/10.36953/ecj.10262239.

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The present study was aimed at the evaluation of soil temperature, pH and carbon dioxide evolution on the number and efficiency of fluorescent pseudomonads around the root system of Pokkali rice at Vytilla in Ernakulam district of Kerala. Two plots (40 m2) comprising control (without application of Pseudomonas fluorescens) and P. fluorescens treated plants were used for the field experiment. The isolates of fluorescent Pseudomonads or Pseudomonas fluorescence were counted and their efficiency was assessed for IAA, ammonia, HCN and siderophore production. Simultaneously, soil temperature, pH, and carbon dioxide evolution were also recorded. A total of 6 fluorescent pseudomonads (VPJU, VPJL, VPAU1, VPAU2, VPAU3 and VPAU4) were found during the crop period. All the isolates produced IAA and ammonia with varying degrees of intensity. Three isolates (VPAU1, VPAU3 and VPAU4) produced HCN, and no microbial isolates produced siderophore. The effect of soil temperature, pH, EC and carbon dioxide evolution was correlated with the number of fluorescent pseudomonads in the soil. The bacteria were significantly afflicted by pH and EC, whereas soil temperature and CO2 evolution did not show any effect on the number of fluorescent pseudomonads. There was no significant influence of soil temperature, pH, EC and carbon dioxide evolution on indole acetic acid production, ammonia, and HCN production. Inoculated Pseudomonas fluorescence did not survive in Pokkali rice fields. However, further studies are needed for at least three seasons in Pokkali soils to confirm the results of the present study.

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Silva, Gildo Almeida da, and Erik Amazonas de Almeida. "Production of yellow-green fluorescent pigment by Pseudomonas fluorescens." Brazilian Archives of Biology and Technology 49, no. 3 (May 2006): 411–19. http://dx.doi.org/10.1590/s1516-89132006000400009.

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A medium was prepared from brewery waste yeast with and without mineral salts to study growth and yellow-green fluorescent pigment production (YGFP) by Pseudomonas fluorescens. The King's medium used for detection of siderophore production were expressively weaker inductors of YGFP formation when compared to FYE medium. Although FYE and CYE could be used for growth of P. fluorescens, only FYE was an attractive medium for detection of YGFP strain producers.

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4

Shu, Huizhen, Haiming Chen, Xiaolong Wang, Yueying Hu, Yonghuan Yun, Qiuping Zhong, Weijun Chen, and Wenxue Chen. "Antimicrobial Activity and Proposed Action Mechanism of 3-Carene against Brochothrix thermosphacta and Pseudomonas fluorescens." Molecules 24, no. 18 (September 6, 2019): 3246. http://dx.doi.org/10.3390/molecules24183246.

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3-Carene is an antimicrobial monoterpene that occurs naturally in a variety of plants and has an ambiguous antibacterial mechanism against food-borne germs. The antibacterial effects and action mechanism of 3-carene against Gram-positive Brochothrix thermosphacta ACCC 03870 and Gram-negative Pseudomonas fluorescens ATCC 13525 were studied. Scanning electron microscopy (SEM) examination and leakage of alkaline phosphatase (AKP) verified that 3-carene caused more obvious damage to the morphology and wall structure of B. thermosphacta than P. fluorescens. The release of potassium ions and proteins, the reduction in membrane potential (MP), and fluorescein diacetate (FDA) staining further confirmed that the loss of the barrier function of the cell membrane and the leakage of cytoplasmic contents were due to the 3-carene treatment. Furthermore, the disorder of succinate dehydrogenase (SDH), malate dehydrogenase (MDH), pyruvate kinase (PK), and ATP content indicated that 3-carene could lead to metabolic dysfunction and inhibit energy synthesis. In addition, the results from the fluorescence analysis revealed that 3-carene could probably bind to bacterial DNA and affect the conformation and structure of genomic DNA. These results revealed that 3-carene had strong antibacterial activity against B. thermosphacta and P. fluorescens via membrane damage, bacterial metabolic perturbations, and genomic DNA structure disruption, interfering in cellular functions and even causing cell death.

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5

Aloysius Ng. Lende, Laurensius Lehar, and Heny MC Sine. "Application of organic fertilizer and Pseudomonas fluorescens on the growth and yield of shallot cultivar Sabu Raijua (Allium ascalonicum L .) in dry land." GSC Advanced Research and Reviews 5, no. 2 (November 30, 2020): 123–30. http://dx.doi.org/10.30574/gscarr.2020.5.2.0105.

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The specific objectives of this study were 1 ) knowing certain types of organic fertilizers on the growth of shallots 2 ) knowing the concentrations of Pseudomonas fluorescenss certain the growth of shallots, 3 ) knowing the types of organic fertilizers and the concentrations Pseudomonas fluorescens specificity increase the optimal yield of shallots. To achieve this goal, this research was conducted using factorial experiments with a split Plot Design with 10 treatments and 3 replications. So that there are 10 treatment combinations of a total number of 30 experimental plots. There were 2 factors that were tried, namely the first factor of Organic Fertilizer as the main plot, namely: cow manure 10 ton ha-1 (K1), chicken manure 10 ton ha-1 (K2). While the second factor as the subplot is the concentration of Pseudomonas fluorescens: Watering with water (as a control) 100 ml (P0), Watering with a concentration of Pseudomonas fluorescens 5 ml + normal water 95 ml (P1), Watering with a concentration of Pseudomonas fluorescens 10 + ordinary water 90 ml (P2), sprinkling with a concentration of Pseudomonas fluorescens 15 ml + 85 ml plain water (P3), Flushing with a concentration of Pseudomonas fluorescens 20 + ordinary water 80 ml (P4). The shallot cultivar of Sabu Raijua which was given organic fertilizer of 10 tonnes of chicken manure. Ha-1 and a concentration of Pseudomonas fluorescens 20 + 80 ml of plain water gave the highest growth component at the age of 10 WAP, namely at the age of 10 WAP, namely plant height (37.667cm). Leaves (34, 800 trees), number of tillers (10, 533 trees). The results of shallot bulbs of Sabu Raijua cultivar from organic fertilizer treatment of 10 ton ha-chicken manure1s with a concentration of Pseudomonas fluorescens 20 ml + 80 ml water resulted in components, namely tuber weight per plot (276.70 g ), number of tubers per plot (291, 70 tubers ).

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6

Dynes, James J., John R. Lawrence, Darren R. Korber, George D. W. Swerhone, Gary G. Leppard, and Adam P. Hitchcock. "Morphological and biochemical changes inPseudomonas fluorescensbiofilms induced by sub-inhibitory exposure to antimicrobial agents." Canadian Journal of Microbiology 55, no. 2 (February 2009): 163–78. http://dx.doi.org/10.1139/w08-109.

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Confocal laser scanning microscopy (CLSM) and scanning transmission X-ray microscopy (STXM) were used to examine the morphological and biochemical changes in Pseudomonas fluorescens biofilms grown in the presence of subinhibitory concentrations of 4 antimicrobial agents: triclosan, benzalkonium chloride, chlorhexidine dihydrochloride, and trisodium phosphate. CLSM analyses using the stains SYTO9 and propidium iodide indicated that the antimicrobial agents affected cell membrane integrity and cellular density to differing degrees. However, fluorescein diacetate assays and plate counts demonstrated that the cells remained metabolically active. Fluorescent lectin binding assays showed that changes in the arrangement and composition of the exopolymer matrix of the biofilms also occurred and that these changes depended on the antimicrobial agent. Detailed single cell analyses using STXM provided evidence that the cell morphology, and the spatial distribution and relative amounts of protein, lipids and polysaccharides in the biofilms and within the cells were different for each antimicrobial. The distribution of chlorhexidine in the biofilm, determined from its distinct spectral signature, was localized mainly inside the bacterial cells. Each antimicrobial agent elicited a unique response; P. fluorescens cells and biofilms changed their morphology and architecture, as well as the distribution and abundance of biomacromolecules, in particular the exopolymer matrix. Pseudomonas fluorescens also exhibited adaptation to benzalkonium chloride at 10 µg/mL. Our observations point to the importance of changes in the quantity and chemistry of the exopolymeric matrix in the response to antimicrobial agents and suggest their importance as targets for control.

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7

Armarkar, Sarika A., R. M. Gade, and Mina D. Koche. "Growth Promotion Activity And Growth Pattern Of Pseudomonas Fluorescens On Different Solid Media." Journal of Plant Disease Sciences 17, no. 1 (August 9, 2022): 22–27. http://dx.doi.org/10.48165/jpds.2022.1706.

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Study was conducted in vitro to study the growth promotion activity and growth pattern of Pseudomonas fluorescens on different solid media. Soil samples were collected randomly from rhizosphere of citrus plants for isolation of Pseudomonas. Twenty six isolates was isolated, out of twenty six isolates eight isolates showed abiliity of siderophore production, thirteen isolates showed positiveness for IAA production, nine isolates showed phosphate solubilization and seven isolates were positive for HCN production. For growth pattern study of P. fluorescens three different media i.e. King’s B, Pseudomonas agar and nutrient agar used. All the isolates showed fast growth on King’s B followed by Pseudomonas agar and nutrient agar. Mostly Pseudomonas fluorescens produced greenish yellow coloured colonies on King’s B and Pseudomonas agar and dull yellowish coloured colony and creamy white coloured colony on nutrient agar. Fluorescent pigmentation was observed on King’B and Pseudomonas agar medium.

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Downing, Katrina J., Graeme Leslie, and Jennifer A. Thomson. "Biocontrol of the Sugarcane Borer Eldana saccharina by Expression of the Bacillus thuringiensis cry1Ac7 andSerratia marcescens chiA Genes in Sugarcane-Associated Bacteria." Applied and Environmental Microbiology 66, no. 7 (July 1, 2000): 2804–10. http://dx.doi.org/10.1128/aem.66.7.2804-2810.2000.

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ABSTRACT The cry1Ac7 gene of Bacillus thuringiensisstrain 234, showing activity against the sugarcane borer Eldana saccharina, was cloned under the control of the tacpromoter. The fusion was introduced into the broad-host-range plasmid pKT240 and the integration vector pJFF350 and without thetac promoter into the broad-host-range plasmids pML122 and pKmM0. These plasmids were introduced into a Pseudomonas fluorescens strain isolated from the phylloplane of sugarcane and the endophytic bacterium Herbaspirillum seropedicae found in sugarcane. The ptac-cry1Ac7 construct was introduced into the chromosome of P. fluorescens using the integration vector pJFF350 carrying the artificial interposon Omegon-Km. Western blot analysis showed that the expression levels of the integratedcry1Ac7 gene were much higher under the control of thetac promoter than under the control of its endogenous promoter. It was also determined that multicopy expression in P. fluorescens and H. seropedicae of ptac-cry1Ac7 carried on pKT240 caused plasmid instability with no detectable protein expression. In H. seropedicae, more Cry1Ac7 toxin was produced when the gene was cloned under the control of the Nmr promoter on pML122 than in the opposite orientation and bioassays showed that the former resulted in higher mortality of E. saccharina larvae than the latter. P. fluorescens 14::ptac-tox resulted in higher mortality of larvae than did P. fluorescens14::tox. An increased toxic effect was observed when P. fluorescens 14::ptac-tox was combined with P. fluorescens carrying the Serratia marcescens chitinase gene chiA, under the control of the tac promoter, integrated into the chromosome.

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Velusamy, Palaniyandi, J. Ebenezar Immanuel, Samuel S. Gnanamanickam, and Linda Thomashow. "Biological control of rice bacterial blight by plant-associated bacteria producing 2,4-diacetylphloroglucinol." Canadian Journal of Microbiology 52, no. 1 (January 1, 2006): 56–65. http://dx.doi.org/10.1139/w05-106.

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Certain plant-associated strains of fluorescent Pseudomonas spp. are known to produce the antimicrobial antibiotic 2,4-diacetylphloroglucinol (DAPG). It has antibacterial, antifungal, antiviral, and antihelminthic properties and has played a significant role in the biological control of tobacco, wheat, and sugar beet diseases. It has never been reported from India and has not been implicated in the biological suppression of a major disease of the rice crop. Here, we report that a subpopulation of 27 strains of plant-associated Pseudomonas fluorescens screened in a batch of 278 strains of fluorescent pseudomonads produced DAPG. The DAPG production was detected by a PCR-based screening method that used primers Phl2a and Phl2b and amplified a 745-bp fragment characteristic of DAPG. HPLC,1H NMR, and IR analyses provided further evidence for its production. We report also that this compound inhibited the growth of the devastating rice bacterial blight pathogen Xanthomonas oryzae pv. oryzae in laboratory assays and suppressed rice bacterial blight up to 59%–64% in net-house and field experiments. Tn5 mutants defective in DAPG production (Phl–) of P. fluorescens PTB 9 were much less effective in their suppression of rice bacterial blight.Key words: biocontrol, 2,4-diacetylphloroglucinol, Pseudomonas fluorescens, rice, Xanthomonas oryzae pv. oryzae.

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10

TAKEUCHI, KAZUE, CLAUDIA M. MATUTE, ASHRAF N. HASSAN, and JOSEPH F. FRANK. "Comparison of the Attachment of Escherichia coli O157:H7, Listeria monocytogenes, Salmonella Typhimurium, and Pseudomonas fluorescens to Lettuce Leaves." Journal of Food Protection 63, no. 10 (October 1, 2000): 1433–37. http://dx.doi.org/10.4315/0362-028x-63.10.1433.

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Attachment of Escherichia coli O157:H7, Listeria monocytogenes, Salmonella Typhimurium, and Pseudomonas fluorescens on iceberg lettuce was evaluated by plate count and confocal scanning laser microscopy (CSLM). Attachment of each microorganism (∼108 CFU/ml) on the surface and the cut edge of lettuce leaves was determined. E. coli O157:H7 and L. monocytogenes attached preferentially to cut edges, while P. fluorescens attached preferentially to the intact surfaces. Differences in attachment at the two sites were greatest with L. monocytogenes. Salmonella Typhimurium attached equally to the two sites. At the surface, P. fluorescens attached in greatest number, followed by E. coli O157:H7, L. monocytogenes, and Salmonella Typhimurium. Attached microorganisms on lettuce were stained with fluorescein isothiocyanate and visualized by CSLM. Images at the surface and the cut edge of lettuce confirmed the plate count data. In addition, microcolony formation by P. fluorescens was observed on the lettuce surface. Some cells of each microorganism at the cut edge were located within the lettuce tissues, indicating that penetration occurred from the cut edge surface. The results of this study indicate that different species of microorganisms attach differently to lettuce structures, and CSLM can be successfully used to detect these differences.

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Dissertations / Theses on the topic "Fluorescens"

1

Blankemeier, Andrew R. "Characterization of Pseudomonas fluorescens Biofilm." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1307731184.

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2

Hamel, Robert D. "Aluminum detoxification mechanisms in Pseudomonas fluorescens." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0025/MQ31433.pdf.

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3

Clements, Richard Steven. "The serological homology of Pseudomonas fluorescens proteases." Thesis, Queensland University of Technology, 1987. https://eprints.qut.edu.au/36720/1/36720_Clements_1987.pdf.

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This investigation evaluated the serological homology of proteases from a number of psychotrophic bacteria consisting largely of Pseudomonas_fluorescens strains. Proteases from 4 reference strains of P.fluorescens were purified and rabbit antiserum generated against each. Preliminary investigations revealed the serological heterology of protease OM82 to proteases N73A, M143A and OMl 86. A total of 54, presumably P.fluorescens strains, were grown and semi-pure protease preparations obtained from each. The immunological cross reactivity of 54 semi-pure protease preparations with each of the 4 antisera was evaluated using Ouchterlony Double Diffusion (ODDT), Single Radial Immunodiffusion (SRID) and an Inhibition Enzyme-Linked Immunosorbent Assay (IELISA). The ODDT and IELISA were qualitative tests however, densitometric analysis of SRID precipitin rings enabled cross reaction quantitation in this assay. Each protease was placed into one of 3 enzymo-serogroups according to. the results from each immunological assay. Proteases which did not cross react with any antisera could not be classified. A recent taxonomic study (O'Connor ~t _ gl., 1986) indicated that investigation ~.fluorescens. produced by non several of the enzymes used in this were not produced by strains of It was generally found that proteases P.fluorescens species did not cross react with reference P.fluorescens proteases. Further development of the IELISA increased sensitivity thus, enabling the detection of low nanogram levels of specific proteases in ultra high temperature treated milk. A number of monoclonal antibodies were produced and used to determine whether a common antigenic determinant existed amongst these proteolytic enzymes . Evi dence presented in this study suggests the existence of such an epitope however, the distribution of this determinant amongst the secreted proteases of P . flyore~.Qens is not known .

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Levasseur, Rémi. "Aluminum citrate transport and metabolism in Pseudomonas fluorescens." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0018/MQ46489.pdf.

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Wan, Dagang Wan Rosmiza Zana. "Understanding adhesion of Pseudomonas fluorescens on household surfaces." Thesis, University of Birmingham, 2012. http://etheses.bham.ac.uk//id/eprint/3821/.

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In this study, three different methods have been used to investigate the bacterial interaction with the substratum, i.e. atomic force microscopy (AFM), spinning disc and micromanipulation. Pseudomonas fluorescens NCIMB 9046 was chosen as a model microorganism to study the cell-substrate adhesion. By having three different colloidal particles: stainless steel (Grade 304), glass and cellulose, the force measurements were performed in growth medium and ambient air using AFM. The results demonstrated that the adhesive forces were influenced by the surface hydrophobicity, electrostatic, van der Waals and steric interactions. In ambient air, the capillary force played an important role. The effect of shear forces on the bacterial adhesion was further examined. By using an apparatus of spinning disc, the cell removal was strongly influenced by the spinning time, angular velocity and surface hydrophobicity. Finally, the adhesive and cohesive strengths of biofilms were examined via a micromanipulation technique. Results indicate that with pH7 and low initial glucose concentration (0.25% (w/v)) the biofilm adhesion was the greatest among the conditions investigated. The cohesive strength of biofilm was found to depend on on the distance between the force probe and the substrate surface.

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Macioszek, Malgorzata. "Biosynthesis of mupirocin by Pseudomonas fluorescens NCIMB 10586." Thesis, University of Birmingham, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.510237.

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Mupirocin, a polyketide antibiotic active against Gram-positive bacteria is used clinically for treatment of bacterial skin infections, to clear Stapylococcus aureus ftom nasal passages and as a surgical scrub to inhibit bacterial growth, particularly that of MRSA. Mupirocin is synthesised by polyketide synthases (PKS) in a series of reactions involving many enzymes encoded by genes from the mupirocin cluster. The mupirocin cluster consists of six larger ORFs (mmpA-F. ) encoding multifunctional proteins, and twenty nine individual genes (mupA-X and macpA-E) all of which have been shown to be required for normal mupirocin biosynthesis and presumably create a biosynthetic assembly line. Sub-groups of mutants produce identical novel metabolites implying the presence of multi-protein complexes. To determine the interactions between proteins of the Mup assembly line Bacterial and Yeast Two Hybrid Systems were used but no evidence has been provided that tested proteins are partners. Although no interaction has been revealed, complementation studies suggest that MupE protein requires coexpression of MupD protein to be functional. Furthermore, inactivation of MupD by amino acid substitution suggests that MupD is not essential on its own for any step in mupirocin biosynthesis except for the proper function of MupE protein. BPLC analysis of wild type P. fluorescens overexpressing mupOlmacpElUIVICIF in trans showed that this did not abolish PA-B production as had been hypothesised but did increase production of total antibiotic (PA-A and PA-B) up to more than two-fold indicating a need to modify our model for the production of PA-B. Results using fluorescence microscopy demonstrate that small Mup proteins are not localized within the bacterial cell and that they are spread evenly in the cells of P. fluorescens.

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Frey-Klett, Pascale. "Ecologie d'un pseudomonas fluorescens auxiliaire de la mycorhization." Paris 11, 1996. http://www.theses.fr/1996PA112480.

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La souche de bacterie auxiliaire de la mycorhization pseudomonas fluorescens bbc6, isolee d'un carpophore de laccaria laccata, stimule l'etablissement de la symbiose entre le douglas et ce champignon ectomycorhizien. Dans le but de comprendre son mode d'action mais aussi pour integrer de facon raisonnee son inoculation aux programmes de mycorhization controlee du douglas, nous avons etudie l'ecologie de la souche bbc6 en serre et en pepiniere. Nous avons compare les caracteristiques phenotypiques et genotypiques de bbc6 a celles de 300 souches de pseudomonas fluorescents isolees du sol nu, de la mycorhizosphere et des mycorhizes de douglas-l. Laccata dans un essai en pepiniere. Bbc6 appartient au biovar i des p. Fluorescens et partage des caracteristiques phenotypiques communes a toutes les souches de p. Fluorescens biovar i isolees de la mycorhizosphere et des mycorhizes. Elle est capable d'utiliser le trehalose, sucre majoritairement accumule dans le mycelium de l. Laccata in vitro. Nous avons d'autre part etudie l'ecologie de bbc6 grace a l'utilisation d'un mutant spontane resistant a la rifampicine. La population bacterienne decroit dans le sol et la rhizosphere des semis de douglas apres l'inoculation. La bacterie n'est donc ni tellurique, ni rhizospherique. Elle n'est pas non plus preferentiellement associee aux mycorhizes ni aux carpophores de l. Laccata. Pourtant, la bacterie survit mieux en presence de l. Laccata. La bacterie pourrait donc etre associee au mycelium du champignon dans le sol et profiter des exsudats fongiques, comme le trehalose par exemple. L'effet auxiliaire de la bacterie se manifeste trois a six semaines apres la formation des premieres mycorhizes, lorsque la densite bacterienne a deja chute d'au moins 1000 fois. Il se manifeste egalement pour des doses d'inoculum bacterien aussi faibles que 10 ufc cm#-#3 de sol et permet de diminuer la dose d'inoculum fongique utilisee sans diminuer l'indice de mycorhization des plants

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Koza, Anna. "Adaptation and niche construction by Pseudomonas fluorescens SBW25." Thesis, Abertay University, 2011. https://rke.abertay.ac.uk/en/studentTheses/7888a5ac-f562-4518-8124-36d2e394994d.

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The mechanisms underlying adaptive radiation or evolution have been extensively investigated using experimental bacterial populations in liquid cultures, referred to as microcosms. Evolving populations of Pseudomonas fluorescens SBW25 in static microcosms reproducibly lead to the emergence of the Wrinkly Spreader (WS) genotype. These produce a cellulose-matrix-based biofilm to colonise the airliquid (A-L) interface with significant fitness advantage over non-biofilm-forming competitors. In this work, the first SBW25 colonists in static microcosms were shown to establish O₂ gradients, thereby modifying the original environment to establish two new niches corresponding to a high-O₂ region at the A-L interface and a low-O₂ region lower down the liquid column. Greater O₂ availability at the A-L interface was found to provide the selective pressure driving the emergence of the WS and underlay the fitness advantage WS have over non-biofilm-forming strains in this environment. A second biofilm-forming mutant of SBW25, known as the CBFS (Complementary Biofilm Forming Strain), had been previously characterised. Here, wild-type SBW25 was shown to produce a third biofilm-type induced non-specifically by exogenous Fe, and referred to as the viscous mass (VM)-class biofilm. The CBFS, VM and WS biofilm-types could be differentiated by in situ measurements of biofilm attachment and strength, rheometry of biofilm samples, and strain characteristics. However, despite these differences, each provided similar levels of fitness in static microcosms subjected to increasing levels of physical disturbance. This suggests that each of the biofilm-types might arise independently in evolving SBW25 populations as the result of convergent evolution, in which the key ecological constraints are O₂ availability and physical disturbance. However, invasion-from-rare experiments indicated that the WS was ecologically more successful, perhaps explaining why only WS-like genotypes have been isolated from evolving SBW25 populations in static microcosms. Other pseudomonads had previously been shown to produce cellulose-based A-L interface biofilms in static microcosms. Here, a survey of New Zealand brown blotch-causing pseudomonads (BCP) recovered from white mushrooms (Agaricus bisporus) identified similar biofilm-producing, cellulose-expressing isolates. The ability to express cellulose by key BCP isolates was shown to be a fitness advantage in static microcosms, as well as on A. bisporus mushroom caps themselves. Cellulose-expression was also found to be of fitness advantage under water-limiting conditions, suggesting that cellulose may provide some resistance to water-stress in the natural environment. The research undertaken for this Thesis comprise several significant advances in our understanding of the adaptive radiation of P. fluorescens SBW25 and the emergence of A-L interface biofilms in static microcosms. This work has identified O₂ gradients and physical disturbance as the dominant environmental factors that guide the convergent evolution of biofilms in this environment. A consequence of convergent evolution in static microcosms is a variety of physically-different biofilms, all of which provide a fitness advantage over non-biofilm-forming competitors. It is also enlightening to realise that a common structural component of biofilms, cellulose, may also be used in another role to provide a fitness advantage in an ecologically more relevant and natural environment.

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Kulkarni, N. "Studies on lipase enzyme from pseudomonas fluorescens NS2W." Thesis(Ph.D.), CSIR-National Chemical Laboratory, Pune, 2002. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/2333.

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10

Wang, Chien-Sao. "Cell-Free Recovery and Isotopic Identification of Cyanide Degrading Enzymes from Pseudomonas Fluorescens." Thesis, University of North Texas, 1995. https://digital.library.unt.edu/ark:/67531/metadc278363/.

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Cell-free extracts from Pseudomonas fluorescens NCIMB 11764 catalyzed the degradation of cyanide into products that included C02, formic acid, formamide and ammonia. Cyanide-degrading activity was localized to cytosolic cell fractions and was observed at substrate concentrations as high as 100 mM. Two cyanide degrading activities were identified by: (i) the determination of reaction products stoichiometries, (ii) requirements for NADH and oxygen, and (iii) kinetic analysis. The first activity produced CO2 and NH3 as reaction products, was dependent on oxygen and NADH for activity, and displayed an apparent Km for cyanide of 1.2 mM. The second activity generated formic acid (and NH3) pfus formamide as reaction products, was oxygen independent, and had an apparent Km of 12 mM for cyanide. The first enzymatic activity was identified as cyanide oxygenase whereas the second activity consists of two enzymes, a cyanide nitrilase (dihydratase) and putative cyanide hydratase. In addition to these enzymes, cyanide-grown cells were also induced for formate dehydrogenase (FDH), providing a means of recycling NADH utilized by cyanide oxygenase.

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Books on the topic "Fluorescens"

1

Yohannes, Berhane. Aluminum efflux in Pseudomonas fluorescens. Sudbury, Ont: Laurentian University, 1997.

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Zarghooni, Maryam. Aluminum malate metabolism in Pseudomonas fluorescens. Sudbury, Ont: Laurentian University, 1999.

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Yonis, Abdoulkader. Interaction entre Sélénium et Pseudomonas fluorescens. Sudbury, Ont: Université Laurentienne, 2002.

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Hamel, Robert D. Aluminum detoxification mechanisms in Pseudomonas fluorescens. Sudbury, Ont: Laurentian University, Chemistry and Biochemistry Department, 1997.

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Levasseur, Rémi. Uptake mechanism of aluminum in "Pseudomonas fluorescens". Sudbury, Ont: Laurentian University, 1997.

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Bédard, Kimberley-Ann. Une pompe active d'aluminium chez Pseudomonas fluorescens. Sudbury, Ont: Université Laurentienne, 1995.

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Anderson, Shawna. Calcium metabolism in pseudomonas fluorescens ATCC 13525. Sudbury, Ont: Laurentian University, 1991.

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Brewer, Guy. Oxidative stress and valine metabolism in pseudomonas fluorescens. Sudbury, Ont: Laurentian University, 2006.

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Anderson, Shawna. Biochemical adaptation to calcium stress in Pseudomonas fluorescens. Sudbury, Ont: Laurentian University, 1995.

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Mader, Rene. Aluminum tolerance in "Pseudmonas fluorescens" in plasmid-mediated. Sudbury, Ont: Laurentian University, 1999.

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Book chapters on the topic "Fluorescens"

1

Chew, Lawrence C., Tom M. Ramseier, Diane M. Retallack, Jane C. Schneider, Charles H. Squires, and Henry W. Talbot. "Pseudomonas fluorescens." In Production of Recombinant Proteins, 45–66. Weinheim, FRG: Wiley-VCH Verlag GmbH & Co. KGaA, 2005. http://dx.doi.org/10.1002/3527603670.ch3.

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Zdorovenko, G. M., S. N. Veremeychenko, I. Ya Zakharova, and Yu A. Knirel. "Endotoxins of Pseudomonas fluorescens." In Endotoxin, 131–35. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4757-5140-6_9.

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Kaur, K., T. R. Bott, and B. S. C. Leadbeater. "Effect of Ozone on Pseudomonas Fluorescens." In Biofilms — Science and Technology, 589–94. Dordrecht: Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-1824-8_52.

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Mavrodi, Dmitri V., Ian T. Paulsen, Qinghu Ren, and Joyce E. Loper. "Genomics of Pseudomonas fluorescens Pf-5." In Pseudomonas, 3–30. Dordrecht: Springer Netherlands, 2007. http://dx.doi.org/10.1007/978-1-4020-6097-7_1.

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Quicke, Peter, Carmel L. Howe, and Amanda J. Foust. "Balancing the Fluorescence Imaging Budget for All-Optical Neurophysiology Experiments." In Neuromethods, 49–74. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2764-8_2.

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AbstractThe goal of this chapter is to establish a framework to evaluate imaging methodologies for all-optical neurophysiology experiments. This is not an exhaustive review of fluorescent indicators and imaging modalities but rather aims to distill the functional imaging principles driving the choice of both. Scientific priorities determine whether the imaging strategy is based on an “optimal fluorescent indicator” or “optimal imaging modality.” The choice of the first constrains the choice of the second due to each’s contributions to the fluorescence budget and signal-to-noise ratio of time-varying fluorescence changes.

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Kalita, Prakash Jyoti, and Ratul Moni Ram. "Industrial Applications of Pseudomonas fluorescens: A Patent Survey." In Intellectual Property Issues in Microbiology, 383–402. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-7466-1_21.

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Seaton, Sarah Craven, and Mark W. Silby. "Genetics and Functional Genomics of the Pseudomonas fluorescens Group." In Genomics of Plant-Associated Bacteria, 99–125. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-55378-3_5.

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Troppens, Danielle M., Jennifer A. Moynihan, Mathieu Barret, Fergal O'Gara, and John P. Morrissey. "Genetics and Evolution of 2, 4-Diacetylphloroglucinol Synthesis inPseudomonas Fluorescens." In Molecular Microbial Ecology of the Rhizosphere, 593–605. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2013. http://dx.doi.org/10.1002/9781118297674.ch56.

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Westphal, A. H., K. Eschrich, W. M. A. M. van Dongen, J. A. E. Benen, A. de Kok, and W. J. H. van Berkel. "SITE-DIRECTED MUTAGENESIS OF PARA-HYDROXYBENZOATE HYDROXYLASE FROM PSEUDOMONAS FLUORESCENS." In Flavins and Flavoproteins 1990, edited by B. Curti, S. Ronchi, and G. Zanetti, 231–34. Berlin, Boston: De Gruyter, 1991. http://dx.doi.org/10.1515/9783110855425-044.

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Herman, Brian. "Fluorescence Microscopy: State of the Art." In Fluorescence Microscopy and Fluorescent Probes, 1–14. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_1.

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Conference papers on the topic "Fluorescens"

1

Armstrong, R. L., J. G. Xie, T. E. Ruskgauer, and R. G. Pinnick. "Energy transfer lasing from dye-doped microdroplets seeded with fluorescent sol." In OSA Annual Meeting. Washington, D.C.: Optica Publishing Group, 1992. http://dx.doi.org/10.1364/oam.1992.tug2.

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We report observations of lasing and fluorescence emission from liquid microdroplets of fluorescein 548 dye in ethanol, seeded with submicrometer sized fluorescent sol. An incident pump laser generates droplet fluorescein lasing or fluorescene, which in turn excites either fluorescene or lasing in the sol. All emissions are at wavelengths corresponding to morphology dependent resonances (MDR's) of the droplet. Studies of the dependence of these emissions on pump laser intensity and sol concentration suggest they are driven by enhanced radiative energy transfer that occurs when fluorescein lasing or fluorescence emission couples to MDR's of the droplet microcavity. Other noteworthy findings include the absence of sol emission for larger sol and the presence of sol emission, even without any observable fluorescein emission, for smaller sol.

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Ananyeva, I. N., Z. M. Aleschenkova, P. V. Rybaltovskaya, and M. A. Chindareva. "Effect of soybean (Glycine max (L.) Merill) treatments on the introduction capacity of endophytic bacteria." In CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-2020-5-9-10-103.

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The goal of the work was to obtain antibiotic-resistant forms of endophytic Glycine max L. (Merill) bacteria and to study their introduction potential affected by different seed treatment methods. Rifampicin-resistant variants of endophytic soybean bacteria Rhizobium radiobacter 27c and Pseudomonas fluorescens 11E preserving valuable properties were derived. Soybean seed treatment with Bradyrhizobium japonicum BIM V-501D and endophytic nitrogen-fixing Rh. radiobacter 27c, phosphate-mobilizing Ps. fluorescens 11E bacteria under model conditions promoted accumulation of nitrogen-fixing bacteria in the root, stem and leaves. The number of nodules rose by 70% compared with the mono-inoculated control; plant height increased by 19%.

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Ferreira, Irlon M., and André L. M. Porto. "Chemoenzymatic synthesis of derivatives azoles by lipase from Pseudomonas fluorescens." In 15th Brazilian Meeting on Organic Synthesis. São Paulo: Editora Edgard Blücher, 2013. http://dx.doi.org/10.5151/chempro-15bmos-bmos2013_2013914154139.

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Shaposhnikov, A. I., N. A. Vishnevskaya, V. Yu Shakhnazarova, D. S. Syrova, E. V. Borodina, O. N. Kovaleva, and O. K. Strunnikova. "The effect of Fusarium culmorum and Pseudomonas fluorescens 2137 on the content of abscisic acid in the roots and shoots of barley seedlings." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.220.

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Inoculation of barley with a F. culmorum not accompanied by an increase in the number of abscisic acid in plants, but under P. fluorescens 2137 inoculation, the accumulation of abscisic acid in the roots occurred.

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Shaposhnikov, A. I., N. A. Vishnevskaya, V. Yu Shakhnazarova, D. S. Syrova, E. V. Borodina, O. N. Kovaleva, and O. K. Strunnikova. "Features of the initial stages of the relationship between barley, phytopathogenic fungus Fusarium culmorum and rhizobacteria Pseudomonas fluorescens 2137." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.219.

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It was shown that enhanced colonization of barley's roots by Fusarium culmorum in the presence of Pseudomonas fluorescens 2137 may be due to the composition of root exudates. Strain 2137 can enhance expression of plant defence gene PAL.

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Соломийчук, М., О. Панимарчук, В. Кушнир, and М. Никорюк. "Сочетание биологического препарата на основе бактерий Pseudomonas Fluorescens и стимулирующих веществ." In International Scientific Symposium "Plant Protection – Achievements and Prospects". Institute of Genetics, Physiology and Plant Protection, Republic of Moldova, 2020. http://dx.doi.org/10.53040/9789975347204.34.

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Derivatives of ammonium salts of dihydropyrimidine did not show a toxic effect on reducing the concentration of viable cells of the bacterium strain AR-33 Pseudomonas fluorescens. The best indicators of the weight of 100 seeds and the number of formed beans in soybeans were shown by the combination Planriz - 5 l / ha + 0.1% solution of xymedon + 0.2% solution of succinic acid + 2 ml of DMAE + 2 ml of DMSO. The use of all combinations of biocomplexes showed the effectiveness of drugs against diseases in the range of 59.31-69.63%. As a result of the use of biocomplexes, their fungicidal, immunoprotective and stimulating action, an increase in yield of 1.15 - 1.7 times relative to control was recorded. The best yield on potatoes showed a combination of Planriz, v.s. (bacteria of strain AP-33 Pseudomonas fluorescens, 3x109 CFU / cm3) - 5 l / ha + 0.1% solution of ximedon + 0.2% solution of succinic acid + 2 ml of DMAE + 2 ml of DMSO, which was 3 , 4 t / ha. The effectiveness of the drug against late blight was 79.1%.

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Aleschenkova, Z. M., P. V. Rybaltovskaya, and I. N. Ananyeva. "Application of nitrogen-fixing and phosphate-mobilizing bacteria to improve the growth-promoting effect of liquid biohumus." In CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-2020-5-9-10-102.

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The goal of the work was to study the effect of introducing nitrogenfixing and phosphate-mobilizing bacteria into liquid biohumus on its growth-promoting properties. The group of ammonifying microorganisms dominates in the structure of microbocenosis of liquid biohumus, constituting 98.46 %. The introduction of nitrogenfixing bacterial strains B. aryabhattai Cp-1 and phosphate-mobilizing Ps. Fluorescens Pr-2 at 10 % concentration in 1 % liquid biohumus (initial pH 9.5) changes pH to 7.8. Winter wheat seed treatment with liquid biohumus enriched with Ps. Fluorescens Pr-2 increases length and dry weight of seedlings by 40.0 and 80.2%; with B. aryabhattai Ср-1 – by 16.3 and 2.0% compared to the control (1% liquid biohumus). Application of liquid biohumus enriched with pseudomonades and bacilli for the treatment of cress-lettuce seeds provides increase of length and crude weight of seedlings by 25.7 and 20.0; 5.0 and 10.0%, respectively.

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CHRISNAWATI, CHRISNAWATI. "Evaluasi antagonis Pseudomonas fluorescens dalam mengendalikan penyakit layu fusarium pada tomat." In Seminar Nasional Masyarakat Biodiversitas Indonesia. Masyarakat Biodiversitas Indonesia, 2017. http://dx.doi.org/10.13057/psnmbi/m030219.

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Zheleznyakov, S. V., V. K. Lebedeva, T. V. Kalinina, A. P. Kozhemyakov, and L. M. Jacobi. "Analysis of Pseudomonas fluorescens inoculation effect on the work of mycorrhiza formed on black medic by arbuscular fungi differing in symbiotic activity." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.288.

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The influence of rhizobacteria Pseudomonas fluorescens, as well as four species of fungi from the Glomeromycota phylum on the productivity of black medic in mono - and double (fungus + bacteria) inoculation was studied. A high dependence of the results on the symbiotic activity of mycosymbiont was established.

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Shaposhnikov, A. I., N. A. Vishnevskaya, V. Yu Shakhnazarova, D. S. Syrova, E. V. Borodina, O. N. Kovaleva, and O. K. Strunnikova. "Activation of protective reactions in barley plants during colonization of roots with the phytopathogenic fungus Fusarium culmorum in the presence of Pseudomonas fluorescens 2137." In CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-2020-5-9-10-118.

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The expression of the PAL gene, one of the host protection genes, in sterile barley plants and colonized F. culmorum and P. fluorescens 2137 were assessed. The obtained results indicate that strain 2137 may cause a more active protective response (1.5-2.1 fold) in barley than a phytopathogenic fungus.

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Reports on the topic "Fluorescens"

1

Daniel P. Molloy. Factors Affecting Zebra Mussel Kill by the Bacterium Pseudomonas fluorescens. Office of Scientific and Technical Information (OSTI), February 2004. http://dx.doi.org/10.2172/876491.

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Frost, John W. Green Synthesis of Phloroglucinol: Exploiting Pseudomonas fluorescens and Scale-Up. Fort Belvoir, VA: Defense Technical Information Center, January 2008. http://dx.doi.org/10.21236/ada593488.

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Frost, John W. Green Synthesis of Phloroglucinol: Exploiting Pseudomonas fluorescens and Scale-Up. Fort Belvoir, VA: Defense Technical Information Center, January 2010. http://dx.doi.org/10.21236/ada548823.

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Frost, John W. Green Synthesis of Phloroglucinol: Exploiting Pseudomonas fluorescens and Scale-Up. Fort Belvoir, VA: Defense Technical Information Center, January 2009. http://dx.doi.org/10.21236/ada548824.

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Frost, John W. Green Synthesis of Phloroglucinol: Exploiting Pseudomonas fluorescens and Scale-Up. Fort Belvoir, VA: Defense Technical Information Center, October 2009. http://dx.doi.org/10.21236/ada548825.

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Daniel Molloy. IMPACT OF SIPHONING ACTIVITY AND NATURALLY SUSPENDED PARTICLE LOAD ON MUSSEL KILL by PSEUDOMONAS FLUORESCENS. Office of Scientific and Technical Information (OSTI), August 2003. http://dx.doi.org/10.2172/822038.

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Daniel P. Molloy. LETHALITY OF PSEUDOMONAS FLUORESCENS STRAIN CLO145A TO THE 2 ZEBRA MUSSEL SPECIES PRESENT IN NORTH AMERICA. Office of Scientific and Technical Information (OSTI), October 2001. http://dx.doi.org/10.2172/811381.

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Thomashow, Linda, Leonid Chernin, Ilan Chet, David M. Weller, and Dmitri Mavrodi. Genetically Engineered Microbial Agents for Biocontrol of Plant Fungal Diseases. United States Department of Agriculture, 2005. http://dx.doi.org/10.32747/2005.7696521.bard.

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The objectives of the project were: a) to construct the site-specific integrative expression cassettes carrying: (i) the chiA gene for a 58-kDa endochitinase, (ii) the pyrrolnitrin biosynthesis operon, and (iii) the acdS gene encoding ACC deaminase; b) to employ these constructs to engineer stable recombinant strains with an expanded repertoire of beneficial activities; c) to evaluate the rhizosphere competence and antifungal activity of the WT and modified strains against pathogenic fungi under laboratory and greenhouse conditions; and d) to monitor the persistence and impact of the introduced strains on culturable and nonculturable rhizosphere microbial populations in the greenhouse and the field. The research generally support our concepts that combining strategically selected genes conferring diverse modes of action against plant pathogens into one organism can improve the efficacy of biological control agents. We hypothesized that biocontrol agents (BCAs) engineered to expand their repertoire of beneficial activities will more effectively control soilborne plant pathogens. In this work, we demonstrated that biocontrol activity of Pseudomonas fluorescens Q8r1-96 and Q2-87, both producing the antibiotic 2,4-diacetylphloroglucinol (2,4-DAPG) effective against the plant pathogenic fungus Rhizoctonia solani, can be improved significantly by introducing and expressing either the 1.6-kb gene chiA, encoding the 58-kDa endochitinase ChiA from the rhizosphere strain SerratiaplymuthicaIC1270, or the 5.8-kb prnABCDoperon encoding the broad-range antibiotic pyrrolnitrin (Prn) from another rhizosphere strain, P. fluorescens Pf-5. The PₜₐcchiAandPₜₐcprnABCDcassettes were cloned into the integrative pBK-miniTn7-ΩGm plasmid, and inserted into the genomic DNA of the recipient bacteria. Recombinant derivatives of strains Q8r1-96 and Q2-87 expressing the PₜₐcchiA or PₜₐcprnABCD cassettes produced endochitinase ChiA, or Prn, respectively, in addition to 2,4-DAPG, and the recombinants gave significantly better biocontrol of R. solani on beans under greenhouse conditions. The disease reduction index increased in comparison to the parental strains Q8r1-96 and Q2-87 to 17.5 and 39.0% from 3.2 and 12.4%, respectively, in the case of derivatives carrying the PₜₐcchiAcassette and to 63.1 and 70% vs. 2.8 and 12,4%, respectively, in the case of derivatives carrying the PₜₐcprnABCDcassette. The genetically modified strains exhibited persistence and non-target effects comparable to those of the parental strains in greenhouse soil. Three integrative cassettes carrying the acdS gene encoding ACC deaminase cloned under the control of different promoters were constructed and tested for enhancement of plant growth promotion by biocontrol strains of P. fluorescens and S. plymuthica. The integrative cassettes constructed in this work are already being used as a simple and efficient tool to improve biocontrol activity of various PGPR bacteria against fungi containing chitin in the cell walls or highly sensitive to Prn. Some parts of the work (e. g., construction of integrative cassettes) was collaborative while other parts e.g., (enzyme and antibiotic activity analyses) were fully synergistic. The US partners isolated and provided to the Israeli collaborators the original biocontrol strains P. fluorescens strains Q8r1-96 and Q2-87 and their mutants deficient in 2,4-DAPG production, which were used to evaluate the relative importance of introduction of Prn, chitinase or ACC deaminase genes for improvement of the biocontrol activity of the parental strains. The recombinant strains obtained at HUJI were supplied to the US collaborators for further analysis.

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Deng, Chun, Zhenyu Zhang, Zhi Guo, Hengduo Qi, Yang Liu, Haimin Xiao, and Xiaojun Li. Assessment of intraoperative use of indocyanine green fluorescence imaging on the number of lymph node dissection during minimally invasive gastrectomy: a systematic review and meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, November 2021. http://dx.doi.org/10.37766/inplasy2021.11.0062.

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Review question / Objective: Whether is indocyanine green fluorescence imaging-guided lymphadenectomy feasible to improve the number of lymph node dissections during radical gastrectomy in patients with gastric cancer undergoing curative resection? Condition being studied: Gastric cancer was the sixth most common malignant tumor and the fourth leading cause of cancer-related death in the world. Radical lymphadenectomy was a standard procedure in radical gastrectomy for gastric cancer. The retrieval of more lymph nodes was beneficial for improving the accuracy of tumor staging and the long-term survival of patients with gastric cancer. Indocyanine green(ICG) near-infrared fluorescent imaging has been found to provide surgeons with effective visualization of the lymphatic anatomy. As a new surgical navigation technique, ICG near-infrared fluorescent imaging was a hot spot and had already demonstrated promising results in the localization of lymph nodes during surgery in patients with breast cancer, non–small cell lung cancer, and gastric cancer. In addition, ICG had increasingly been reported in the localization of tumor, lymph node dissection, and the evaluation of anastomotic blood supply during radical gastrectomy for gastric cancer. However, it remained unclear whether ICG fluorescence imaging would assist surgeons in performing safe and sufficient lymphadenectomy.

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Lindow, Steven E., Shulamit Manulis, Dan Zutra, and Dan Gaash. Evaluation of Strategies and Implementation of Biological Control of Fire Blight. United States Department of Agriculture, July 1993. http://dx.doi.org/10.32747/1993.7568106.bard.

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The main objective of this study was to develop data that would facilitate a consistently effective method of biological control of fire blight disease to be developed and to enable its implementation for disease control by ensuring its compatibility with variations in the biological, environmental, and chemical conditions present in pear orchards. As considerable information on the pathogen and biological control of fire blight was already gathered from studies in California and elsewhere, an emphasis was placed on investigating the genetics and ecology of Erwinia amylovora, the causal agent of fire blight in Israel. Studies of plasmid profile, virulence on several host, serological characteristics, as well as DNA fingerprints with selected primers all revealed E. amylovora strains in Israel to be homogeneous. Strains did vary in their resistance to streptomycin, with those from more northern locations being resistant while those in the southern costal plain were all sensitive to streptomycin. Resistance appeared to be conferred by chromosomal mutations as in streptomycin-resistant strains in California. The biological control agent Pseudomonas fluorescens strain A506 colonized flowers of both the Costia and Spodona pear cultivars in Israel as well as Bartlett pear in California. Flowers that were open at the time of spray inoculation of trees subsequently harbored from 105 to 107 cells of strain A506 per flower, while those that opened subsequent to spraying developed population sizes of about 105 cells/flower within 5 days. The incidence of fire blight infections were reduced about 3-fold in several trials in which moderate amounts of disease occurred in the plot areas; this degree of biological control is similar to that observed in California and elsewhere. On two occasions warm and moist weather that favored disease led to epidemics in which nearly all flowers became infected and which was so severe that neither P. fluorescens strain A506 nor chemical bactericides reduced disease incidence. A novel method for identifying antagonistic microorganisms for biological control of fire blight and other diseases was developed. A bacterial ice nucleation gene was introduced into E. amylovora to confer an Ice+ phenotype and the population sizes of this modified pathogen on flowers that had been pre-treated with potential control agents was estimated by measuring the freezing temperature of colonized flowers. Antagonistic strains that prevented the growth of E. amylovora in flowers were readily detected as those in which flowers froze at a low temperature. The method is both rapid and unbiased and several bacterial strains with substantial biological control potential have been identified using this method.

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