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Journal articles on the topic 'Fluorescens'

Author: Grafiati

Published: 4 June 2021

Last updated: 26 July 2025

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1

Etebarian, Hassan-Reza, Peter L. Sholberg, Kenneth C. Eastwell, and Ronald J. Sayler. "Biological control of apple blue mold withPseudomonas fluorescens." Canadian Journal of Microbiology 51, no. 7 (2005): 591–98. http://dx.doi.org/10.1139/w05-039.

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Pseudomonas fluorescens isolate 1100-6 was evaluated as a potential biological control agent for apple blue mold caused by Penicillium expansum or Penicillium solitum. Both the wild-type isolate 1100-6 and a genetically modified derivative labeled with the gene encoding the green fluorescent protein (GFP) were compared. The P. fluorescens isolates with or without GFP equally reduced the growth of Penicillium spp. and produced large zones of inhibition in dual culture plate assays. Cell-free metabolites produced by the bacterial antagonists reduced the colony area of Penicillium isolates by 17.

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2

Gopal, Surendra, Reshma Francis, and A. K. Sreelatha. "Impact of soil temperature, pH and carbon dioxide on the population and efficiency of fluorescent pseudomonad in the rhizosphere soil of Pokkali rice." Environment Conservation Journal 24, no. 1 (2023): 163–70. http://dx.doi.org/10.36953/ecj.10262239.

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The present study was aimed at the evaluation of soil temperature, pH and carbon dioxide evolution on the number and efficiency of fluorescent pseudomonads around the root system of Pokkali rice at Vytilla in Ernakulam district of Kerala. Two plots (40 m2) comprising control (without application of Pseudomonas fluorescens) and P. fluorescens treated plants were used for the field experiment. The isolates of fluorescent Pseudomonads or Pseudomonas fluorescence were counted and their efficiency was assessed for IAA, ammonia, HCN and siderophore production. Simultaneously, soil temperature, pH, a

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3

Silva, Gildo Almeida da, and Erik Amazonas de Almeida. "Production of yellow-green fluorescent pigment by Pseudomonas fluorescens." Brazilian Archives of Biology and Technology 49, no. 3 (2006): 411–19. http://dx.doi.org/10.1590/s1516-89132006000400009.

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A medium was prepared from brewery waste yeast with and without mineral salts to study growth and yellow-green fluorescent pigment production (YGFP) by Pseudomonas fluorescens. The King's medium used for detection of siderophore production were expressively weaker inductors of YGFP formation when compared to FYE medium. Although FYE and CYE could be used for growth of P. fluorescens, only FYE was an attractive medium for detection of YGFP strain producers.

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4

Aloysius Ng. Lende, Laurensius Lehar, and Heny MC Sine. "Application of organic fertilizer and Pseudomonas fluorescens on the growth and yield of shallot cultivar Sabu Raijua (Allium ascalonicum L .) in dry land." GSC Advanced Research and Reviews 5, no. 2 (2020): 123–30. http://dx.doi.org/10.30574/gscarr.2020.5.2.0105.

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The specific objectives of this study were 1 ) knowing certain types of organic fertilizers on the growth of shallots 2 ) knowing the concentrations of Pseudomonas fluorescenss certain the growth of shallots, 3 ) knowing the types of organic fertilizers and the concentrations Pseudomonas fluorescens specificity increase the optimal yield of shallots. To achieve this goal, this research was conducted using factorial experiments with a split Plot Design with 10 treatments and 3 replications. So that there are 10 treatment combinations of a total number of 30 experimental plots. There were 2 fact

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5

Shu, Huizhen, Haiming Chen, Xiaolong Wang, et al. "Antimicrobial Activity and Proposed Action Mechanism of 3-Carene against Brochothrix thermosphacta and Pseudomonas fluorescens." Molecules 24, no. 18 (2019): 3246. http://dx.doi.org/10.3390/molecules24183246.

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3-Carene is an antimicrobial monoterpene that occurs naturally in a variety of plants and has an ambiguous antibacterial mechanism against food-borne germs. The antibacterial effects and action mechanism of 3-carene against Gram-positive Brochothrix thermosphacta ACCC 03870 and Gram-negative Pseudomonas fluorescens ATCC 13525 were studied. Scanning electron microscopy (SEM) examination and leakage of alkaline phosphatase (AKP) verified that 3-carene caused more obvious damage to the morphology and wall structure of B. thermosphacta than P. fluorescens. The release of potassium ions and protein

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6

Dynes, James J., John R. Lawrence, Darren R. Korber, George D. W. Swerhone, Gary G. Leppard, and Adam P. Hitchcock. "Morphological and biochemical changes inPseudomonas fluorescensbiofilms induced by sub-inhibitory exposure to antimicrobial agents." Canadian Journal of Microbiology 55, no. 2 (2009): 163–78. http://dx.doi.org/10.1139/w08-109.

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Confocal laser scanning microscopy (CLSM) and scanning transmission X-ray microscopy (STXM) were used to examine the morphological and biochemical changes in Pseudomonas fluorescens biofilms grown in the presence of subinhibitory concentrations of 4 antimicrobial agents: triclosan, benzalkonium chloride, chlorhexidine dihydrochloride, and trisodium phosphate. CLSM analyses using the stains SYTO9 and propidium iodide indicated that the antimicrobial agents affected cell membrane integrity and cellular density to differing degrees. However, fluorescein diacetate assays and plate counts demonstra

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7

Downing, Katrina J., Graeme Leslie, and Jennifer A. Thomson. "Biocontrol of the Sugarcane Borer Eldana saccharina by Expression of the Bacillus thuringiensis cry1Ac7 andSerratia marcescens chiA Genes in Sugarcane-Associated Bacteria." Applied and Environmental Microbiology 66, no. 7 (2000): 2804–10. http://dx.doi.org/10.1128/aem.66.7.2804-2810.2000.

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ABSTRACT The cry1Ac7 gene of Bacillus thuringiensisstrain 234, showing activity against the sugarcane borer Eldana saccharina, was cloned under the control of the tacpromoter. The fusion was introduced into the broad-host-range plasmid pKT240 and the integration vector pJFF350 and without thetac promoter into the broad-host-range plasmids pML122 and pKmM0. These plasmids were introduced into a Pseudomonas fluorescens strain isolated from the phylloplane of sugarcane and the endophytic bacterium Herbaspirillum seropedicae found in sugarcane. The ptac-cry1Ac7 construct was introduced into the ch

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8

Armarkar, Sarika A., R. M. Gade, and Mina D. Koche. "Growth Promotion Activity And Growth Pattern Of Pseudomonas Fluorescens On Different Solid Media." Journal of Plant Disease Sciences 17, no. 1 (2022): 22–27. http://dx.doi.org/10.48165/jpds.2022.1706.

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Study was conducted in vitro to study the growth promotion activity and growth pattern of Pseudomonas fluorescens on different solid media. Soil samples were collected randomly from rhizosphere of citrus plants for isolation of Pseudomonas. Twenty six isolates was isolated, out of twenty six isolates eight isolates showed abiliity of siderophore production, thirteen isolates showed positiveness for IAA production, nine isolates showed phosphate solubilization and seven isolates were positive for HCN production. For growth pattern study of P. fluorescens three different media i.e. King’s B, Pse

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9

Velusamy, Palaniyandi, J. Ebenezar Immanuel, Samuel S. Gnanamanickam, and Linda Thomashow. "Biological control of rice bacterial blight by plant-associated bacteria producing 2,4-diacetylphloroglucinol." Canadian Journal of Microbiology 52, no. 1 (2006): 56–65. http://dx.doi.org/10.1139/w05-106.

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Certain plant-associated strains of fluorescent Pseudomonas spp. are known to produce the antimicrobial antibiotic 2,4-diacetylphloroglucinol (DAPG). It has antibacterial, antifungal, antiviral, and antihelminthic properties and has played a significant role in the biological control of tobacco, wheat, and sugar beet diseases. It has never been reported from India and has not been implicated in the biological suppression of a major disease of the rice crop. Here, we report that a subpopulation of 27 strains of plant-associated Pseudomonas fluorescens screened in a batch of 278 strains of fluor

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10

TAKEUCHI, KAZUE, CLAUDIA M. MATUTE, ASHRAF N. HASSAN, and JOSEPH F. FRANK. "Comparison of the Attachment of Escherichia coli O157:H7, Listeria monocytogenes, Salmonella Typhimurium, and Pseudomonas fluorescens to Lettuce Leaves." Journal of Food Protection 63, no. 10 (2000): 1433–37. http://dx.doi.org/10.4315/0362-028x-63.10.1433.

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Attachment of Escherichia coli O157:H7, Listeria monocytogenes, Salmonella Typhimurium, and Pseudomonas fluorescens on iceberg lettuce was evaluated by plate count and confocal scanning laser microscopy (CSLM). Attachment of each microorganism (∼108 CFU/ml) on the surface and the cut edge of lettuce leaves was determined. E. coli O157:H7 and L. monocytogenes attached preferentially to cut edges, while P. fluorescens attached preferentially to the intact surfaces. Differences in attachment at the two sites were greatest with L. monocytogenes. Salmonella Typhimurium attached equally to the two s

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11

Hunjan, Mandeep Singh, Anjali Thakur, and Pushpinder Paul Singh. "Identification and characterization of Pseudomonas fluorescensstrains effective against Xanthomonasoryzaepv. oryzae causing bacterial blight of rice in Punjab, India." Journal of Applied and Natural Science 9, no. 1 (2017): 253–61. http://dx.doi.org/10.31018/jans.v9i1.1181.

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For the control of bacterial blight of rice caused by Xanthomonasoryzaepv. oryzae, sixty four Pseudomonas fluorescens strains were recovered from rice and wheat rhizosphere. These strains were identified on the basis of internal transcribed spacer (ITS) region. It was observed that the strains showing fluorescence in the selective media showed the amplification of the targeted P. fluorescens specific ITS region. The strains were also characterized for the production of the antibiotic 2, 4-diacetylphloroglucinol (DAPG) using phlDlocus. The characteristic 750bp region was amplified in all the DA

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12

Yee, Dennis C., Jennifer A. Maynard, and Thomas K. Wood. "Rhizoremediation of Trichloroethylene by a Recombinant, Root-Colonizing Pseudomonas fluorescensStrain Expressing Toluene ortho-Monooxygenase Constitutively." Applied and Environmental Microbiology 64, no. 1 (1998): 112–18. http://dx.doi.org/10.1128/aem.64.1.112-118.1998.

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ABSTRACT Trichloroethylene (TCE) was removed from soils by using a wheat rhizosphere established by coating seeds with a recombinant, TCE-degrading Pseudomonas fluorescens strain that expresses the tomA + (tolueneo-monooxygenase) genes from Burkholderia cepacia PR123(TOM23C). A transposon integration vector was used to insert tomA +into the chromosome of P. fluorescens 2-79, producing a stable strain that expressed constitutively the monooxygenase at a level of 1.1 nmol/min · mg of protein (initial TCE concentration, 10 μM, assuming that all of the TCE was in the liquid) for more than 280 cell

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13

Nielsen, T. H., D. Sørensen, C. Tobiasen, et al. "Antibiotic and Biosurfactant Properties of Cyclic Lipopeptides Produced by Fluorescent Pseudomonas spp. from the Sugar Beet Rhizosphere." Applied and Environmental Microbiology 68, no. 7 (2002): 3416–23. http://dx.doi.org/10.1128/aem.68.7.3416-3423.2002.

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ABSTRACT Cyclic lipopeptides (CLPs) with antibiotic and biosurfactant properties are produced by a number of soil bacteria, including fluorescent Pseudomonas spp. To provide new and efficient strains for the biological control of root-pathogenic fungi in agricultural crops, we isolated approximately 600 fluorescent Pseudomonas spp. from two different agricultural soils by using three different growth media. CLP production was observed in a large proportion of the strains (approximately 60%) inhabiting the sandy soil, compared to a low proportion (approximately 6%) in the loamy soil. Chemical s

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14

Lee, Spencer, and Robert Jonas. "Genomic Evolution in Pseudomonas fluorescens as a Result of Gradual Temperature Changes." Fine Focus 9, no. 1 (2023): 84–96. http://dx.doi.org/10.33043/ff.9.1.84-96.

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As climate change continues to affect global temperatures, organisms will need to not only adapt but evolve to survive the changing climate conditions. Temperature selection experiments were performed on Pseudomonas fluorescens to select for growth at lower temperatures. The P. fluorescens temperature selection experiment selected for cells that can grow at a new minimum temperature which is over 20˚C lower than the optimal growth temperature (25-30˚C). Previous experiments established the low end of P. fluorescens’s growth temperature as 4˚C. The genomes of the newly selected and reference st

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15

Dalterio, R. A., W. H. Nelson, D. Brut, J. F. Sperry, J. F. Tanguay, and S. L. Suib. "The Steady-State and Decay Characteristics of Primary Fluorescence from Live Bacteria." Applied Spectroscopy 41, no. 2 (1987): 234–41. http://dx.doi.org/10.1366/000370287774986804.

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The intrinsic steady-state fluorescence and fluorescence decay of Staphylococcus epidermidis, Pseudomonas fluorescens, Enterobacter cloacae, Escherichia coli, and Bacillus subtilis have been observed. Excitation spectra were obtained while emission at 430, 455, 487 and 514 nm was being monitored. Emission spectra were obtained with the use of excitation wavelengths of 340, 365, 405, 430 and 460 nm. Fluorescence lifetimes were measured at 430, 487, and 514 nm while selective excitation was caused at 340, 405, and 430 nm. The complex nature of the excitation and emission spectra reflects the pre

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16

Mohan, Vathsala, Reginald Wibisono, Saili Chalke, Graham Fletcher, and Françoise Leroi. "The Anti-Listeria Activity of Pseudomonas fluorescens Isolated from the Horticultural Environment in New Zealand." Pathogens 12, no. 2 (2023): 349. http://dx.doi.org/10.3390/pathogens12020349.

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Beneficial bacteria with antibacterial properties are attractive alternatives to chemical-based antibacterial or bactericidal agents. Our study sourced such bacteria from horticultural produce and environments to explore the mechanisms of their antimicrobial properties. Five strains of Pseudomonas fluorescens were studied that possessed antibacterial activity against the pathogen Listeria monocytogenes. The vegetative culture of these strains (Pseudomonas fluorescens-PFR46I06, Pseudomonas fluorescens-PFR46H06, Pseudomonas fluorescens-PFR46H07, Pseudomonas fluorescens-PFR46H08 and Pseudomonas f

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17

Hasanuddin, Hasanuddin. "UJI AKTIVITAS ANTIBIOSIS PSEUDOMONADS PENDARFLUOR TERHADAP Rigidoporus lignosus (Klotszch) Imazeki PENYEBAB PENYAKIT AKAR PUTIH." Jurnal Hama dan Penyakit Tumbuhan Tropika 11, no. 1 (2011): 87–94. http://dx.doi.org/10.23960/j.hptt.11187-94.

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The potential of fluorescent bacteria as biological control agents for white root disease caused by Rigidoporus lignosus has been investigated. Isolation of bacteria from the soil using S1 media produced two fluorescent bacteria isolates. Using the Microbact 12A+12B method, both bacteria were identified as Pseudomonas fluorescens and P. aeruginosa. These two species of bacteria were then used as antibiosis activity test against R. lignosus. Four series antibiosis activity tests were done, that were antibiosis test of media culture bacteria growth to R. lignosus colony, antibiosis test of dry f

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18

Istiqomah, Istiqomah, and Dian Eka Kusumawati. "Pemanfaatan Bacillus subtilis dan Pseudomonas fluorescens dalam pengendalian hayati Ralstonia solanacearum penyebab penyakit layu bakteri pada tomat." Jurnal Agro 5, no. 1 (2018): 1–12. http://dx.doi.org/10.15575/2305.

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Salah satu penyakit penting pada produksi tomat di Indonesia adalah layu bakteri yang disebabkan oleh Ralstonia solanacearum. Alternatif untuk mengendalikan penyakit layu bakteri adalah dengan menggunakan Bacillus subtilis dan Pseudomonas fluorescens. Tujuan penelitian ini untuk mengetahui kemampuan B. subtilis dan P. fluorescens dalam mengendalikan penyakit layu bakteri yang disebabkan R. solanacearum serta mekanisme penghambatannya. Penelitian ini terdiri dari 5 tahap, yaitu perbanyakan inokulum R. solanacearum, uji virulensi dan uji hipersensitif R. solanacearum, uji antagonis B. subtilis d

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19

Pradhipta, Hatyanta Nuha, Irianti Kurniasari, and Ugik Romadi. "EFEKTIVITAS PLANT GROWTH PROMOTING RHIZOBACTERIA Pseudomonas fluorescens DALAM PENGENDALIAN HAYATI PENYAKIT BULAI PADA TANAMAN JAGUNG (Zea mays L.)." Agrin 23, no. 1 (2019): 45. http://dx.doi.org/10.20884/1.agrin.2019.23.1.427.

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Bulai merupakan penyakit utama pada tanaman jagung yang disebabkan cendawan patogen Peronosclerospora maydis. P. maydis menyebabkan potensi kehilangan hasil mencapai 100%. Berbagai pengendalian yang dilakukan belum memberikan hasil yang optimal dan bahkan menimbulkan dampak negatif terhadap lingkungan dan kesehatan manusia. PGPR Pseudomonas fluorescens diketahui berpotensi sebagai pengendali penyakit bulai yang ramah lingkungan. Penelitian ini bertujuan untuk mengetahui efektivitas P. fluorescens terhadap penekanan penyakit bulai pada tanaman jagung. Penelitian ini menggunakan Rancangan Acak K

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20

Kjærgaard, Kristian, Mark A. Schembri, Henrik Hasman, and Per Klemm. "Antigen 43 from Escherichia coli Induces Inter- and Intraspecies Cell Aggregation and Changes in Colony Morphology of Pseudomonas fluorescens." Journal of Bacteriology 182, no. 17 (2000): 4789–96. http://dx.doi.org/10.1128/jb.182.17.4789-4796.2000.

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ABSTRACT Antigen 43 (Ag43) is a surface-displayed autotransporter protein ofEscherichia coli. By virtue of its self-association characteristics, this protein is able to mediate autoaggregation and flocculation of E. coli cells in static cultures. Additionally, surface display of Ag43 is associated with a distinct frizzy colony morphology in E. coli. Here we show that Ag43 can be expressed in a functional form on the surface of the environmentally important Pseudomonas fluorescens strain SBW25 with ensuing cell aggregation and frizzy colony types. Using green fluorescence protein-tagged cells,

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21

Sruthy M, Sruthy M., and Shivangi S. Kansara. "Evaluation of Bioagents as Seed Treatment on Dominant Seed Mycoflora of Chilli Var. Gvc 111 In Vitro." Current Agriculture Research Journal 10, no. 1 (2022): 20–27. http://dx.doi.org/10.12944/carj.10.1.04.

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Study to check the efficacy of seed treatment by bioagents on the seed germination and vigour by inhibiting the most dominant seed mycoflora (A. niger, Colletotrichum sp. and Fusarium sp.) of chilli variety GVC 111 was carried out by paper towel method in vitro. Treatment of seeds with T. harzianum+P. fluorescens (5+6g/kg seeds) and P. fluorescens (6g/kg seeds) found effective in seeds pretreated with A. niger, while T. harzianum+P. fluorescens (5+6g/kg seeds) and P. fluorescens+B. subtilis (6+6g/kg seeds) found effective in seeds pretreated with Colletotrichum sp. and P. fluorescens+B. subtil

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22

Cui, Xiaohui, and Rob Harling. "Evaluation of Bacterial Antagonists for Biological Control of Broccoli Head Rot Caused by Pseudomonas fluorescens." Phytopathology® 96, no. 4 (2006): 408–16. http://dx.doi.org/10.1094/phyto-96-0408.

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Pectolytic strains of Pseudomonas fluorescens are opportunistic pathogens of broccoli, causing head rot in temperate regions of the world. In this study, we investigated the potential of two bacterial isolates, P. fluorescens m6418 and Bacillus sp. A24, for biological control of broccoli head rot caused by P. fluorescens 5064, isolated from diseased broccoli in Scotland, UK. P. fluorescens m6418, a Tn5 mutant of wild-type 5064, is nonpathogenic and overproduces an extracellular metabolite with strong antimicrobial activity. In this study, we identified the anti-microbial metabolite produced by

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23

Muarifah, Mizar, Loekas Soesanto, Murti Wisnu Ragil Sastyawan, Endang Mugiastuti, and Noor Farid. "Secondary Metabolites Application of Two Pseudomonas fluorescens isolates and Two Trichoderma Harzianum Isolates in Combination Against Postharvest Anthracnose in Papaya." PLANTA TROPIKA 11, no. 2 (2023): 150–59. http://dx.doi.org/10.18196/pt.v11i2.14594.

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The occurrence of papaya anthracnose is a significant post-harvest ailment, necessitating the effective disease management. The aim was to determine the ability of secondary metabolites combination of Pseudomonas fluorescens and Trichoderma harzianum isolates against the disease. A completely randomized design was used for in vitro experiments and a randomized block design for in vivo experiments. The treatments consisted of P. fluorescens P60 and T. harzianum T10, P. fluorescens P60 and T. harzianum T213, P. fluorescens P32 and T. harzianum T10, P. fluorescens P32 and T. harzianum T213 second

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24

Tomer, Ajay, Ramji Singh, Durga Prasad, and Saurabh Kumar Singh. "INFLUENCE OF SEED BIOPRIMING WITH DIFFERENT ISOLATES OF PSEUDOMONAS FLUORESCENS ON THE GROWTH OF PADDY." Journal of Biopesticides 13, no. 02 (2020): 103–9. http://dx.doi.org/10.57182/jbiopestic.13.2.103-109.

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ABSTRACT Many strains of the Pseudomonas fluorescens have been characterized as plant growth promoting rhizobacteria (PGPR) and they enhance the growth and yield of economical important paddy crops. The effect of bio-priming on paddy paddy seed growth was assessed at Crop Research Centre S.V.B.P.A.&T Meerut India by studying the effect of fluorescent Pseudomonas strains for their growth stimulatory effect on paddy plants in Randomized Block Design (RBD) in pot conditions. A total of nineteen isolates of the rhizobacteria Pseudomonas fluorescens were selected from the fields of paddy, wheat

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25

Kristoffersen, Arne Skodvin. "Fotosyntese og fluorescens." Naturen 142, no. 04 (2018): 157–62. http://dx.doi.org/10.18261/issn.1504-3118-2018-04-04.

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26

Gottlieb, Tom, Glenn Funnell, and Iain Gosbell. "Pseudomonas fluorescens pseudobacteraemia." Medical Journal of Australia 155, no. 11-12 (1991): 854–55. http://dx.doi.org/10.5694/j.1326-5377.1991.tb94085.x.

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27

Fabia, Benedict-Uy, Joshua Bingwa, Jiyeon Park, Nguyen-Mihn Hieu, and Jung-Hoon Ahn. "Utilizing the ABC Transporter for Growth Factor Production by fleQ Deletion Mutant of Pseudomonas fluorescens." Biomedicines 9, no. 6 (2021): 679. http://dx.doi.org/10.3390/biomedicines9060679.

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Pseudomonas fluorescens, a gram-negative bacterium, has been proven to be a capable protein manufacturing factory (PMF). Utilizing its ATP-binding cassette (ABC) transporter, a type I secretion system, P. fluorescens has successfully produced recombinant proteins. However, besides the target proteins, P. fluorescens also secretes unnecessary background proteins that complicate protein purification and other downstream processes. One of the background proteins produced in large amounts is FliC, a flagellin protein. In this study, the master regulator of flagella gene expression, fleQ, was delet

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Idei, Akiko, Eri Kawai, Hiroyuki Akatsuka, and Kenji Omori. "Cloning and Characterization of thePseudomonas fluorescens ATP-Binding Cassette Exporter, HasDEF, for the Heme Acquisition Protein HasA." Journal of Bacteriology 181, no. 24 (1999): 7545–51. http://dx.doi.org/10.1128/jb.181.24.7545-7551.1999.

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ABSTRACT Two ATP-binding cassette (ABC) exporters are present inPseudomonas fluorescens no. 33; one is the recently reported AprDEF system and the other is HasDEF, which exports a heme acquisition protein, HasA. The hasDEF genes were cloned by DNA hybridization with a DNA probe coding for the LipB protein, one of the components of the Serratia marcescens ABC exporter Lip system. P. fluorescens HasA showed sequence identity of 40 to 49% with HasA proteins from Pseudomonas aeruginosa andSerratia marcescens. The P. fluorescens Has exporter secreted HasA proteins from P. fluorescens andP. aerugino

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29

Srivastava, Alok K., Tanuja Singh, T. K. Jana, and Dilip K. Arora. "Induced resistance and control of charcoal rot in Cicer arietinum (chickpea) by Pseudomonas fluorescens." Canadian Journal of Botany 79, no. 7 (2001): 787–95. http://dx.doi.org/10.1139/b01-054.

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Pseudomonas fluorescens isolate 4-92 induced systemic resistance against charcoal rot disease in chickpea (Cicer arietinum L.) caused by Macrophomina phaseolina (Tassi) Goidanich. Time-course accumulation of pathogenesis-related (PR) proteins (chitinases and glucanases) in chickpea plants inoculated with P. fluorescens was significantly (P = 0.05) higher than in control plants. The level of chitinases and glucanases increased by 6.6- to 7-fold up to 4 days postinoculation; thereafter, little decrease in the activity of PR proteins was observed. Root-colonizing populations of P. fluorescens wer

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Wang, Zhirong, Mengyao Jiang, Kewei Chen, et al. "Biocontrol of Penicillium digitatum on Postharvest Citrus Fruits by Pseudomonas fluorescens." Journal of Food Quality 2018 (November 14, 2018): 1–10. http://dx.doi.org/10.1155/2018/2910481.

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The effectiveness of the bacteria antagonist Pseudomonas fluorescens to control green mold caused by Penicillium digitatum on oranges (Citrus sinensis Osbeck, cv. Jincheng) and the possible modes of action were evaluated. Whether in vitro or in vivo, treatments with cell-free autoclaved cultures or culture filtrate had limited capacity to suppress P. digitatum, while P. digitatum was significantly inhibited by bacterial fluid (P. fluorescens in the nutrient broth liquid medium) and bacterial suspension (P. fluorescens in sterile distilled water) with living cells. There was a positive relation

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HU, LIANXIA, SHUFEI ZHANG, YULING XUE, et al. "Rapid Identification of Pseudomonas fluorescens Harboring Thermostable Alkaline Protease by Real-Time Loop-Mediated Isothermal Amplification." Journal of Food Protection 85, no. 3 (2021): 414–23. http://dx.doi.org/10.4315/jfp-21-272.

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ABSTRACT Thermostable alkaline protease (TAP) harbored by Pseudomonas fluorescens decomposes protein in milk and dairy products, leading to milk and dairy product spoilage during storage. Thus, a specific, sensitive, rapid, and simple method is required to detect TAP-harboring P. fluorescens. Two sets of primers targeting the aprX and gyrB genes of P. fluorescens were designed. The detection system and conditions were optimized, and a real-time loop-mediated isothermal amplification (real-time LAMP) method was developed for the simultaneous detection of TAP-harboring P. fluorescens in two sepa

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Liu, Xuemei, Kieran J. Germaine, David Ryan, and David N. Dowling. "DEVELOPMENT OF A GFP‐BASED BIOSENSOR FOR DETECTING THE BIOAVAILABILITY AND BIODEGRADATION OF POLYCHLORINATED BIPHENYLS (PCBS)." JOURNAL OF ENVIRONMENTAL ENGINEERING AND LANDSCAPE MANAGEMENT 15, no. 4 (2007): 261–68. http://dx.doi.org/10.3846/16486897.2007.9636939.

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Two whole-cell biosensors were constructed to detect the in situ biodegradation of polychlorinated biphenyl by chromosomal insertion of a mini‐Tn5‐Kmr‐Pm::gfp[mut3]‐T0‐T1 construct into P. fluorescens. In vitro tests showed that the expression of the Pm promoter depended on the growth phase of the biosensors and the concentration of chemical inducers; chlorinated benzoic acid derivatives. A linear relationship between the fluorescent intensity and the log10 concentration of the inducer was observed. One biosensor (F113L::1180gfp) had the ability to degrade PCBs to relevant chlorobenzoic acid d

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FETT, WILLIAM F. "Inhibition of Salmonella enterica by Plant-Associated Pseudomonads In Vitro and on Sprouting Alfalfa Seed†." Journal of Food Protection 69, no. 4 (2006): 719–28. http://dx.doi.org/10.4315/0362-028x-69.4.719.

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Foodborne illness due to the consumption of contaminated raw or lightly cooked sprouts is a continuing food safety concern. In this study, we tested several plant-associated pseudomonads for their ability to inhibit the growth of Salmonella enterica both in vitro and in situ. An agar spot bioassay method was used with three different media. Only Pseudomonas fluorescens 2-79 produced clear zones of inhibition when tested against five serovars of S. enterica, and activity was dependent on media type and serovar. The antibiosis by derivative strains of P. fluorescens 2-79 defective in the product

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Lowder, M., A. Unge, N. Maraha, J. K. Jansson, J. Swiggett, and J. D. Oliver. "Effect of Starvation and the Viable-but-Nonculturable State on Green Fluorescent Protein (GFP) Fluorescence in GFP-TaggedPseudomonas fluorescens A506." Applied and Environmental Microbiology 66, no. 8 (2000): 3160–65. http://dx.doi.org/10.1128/aem.66.8.3160-3165.2000.

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ABSTRACT The green fluorescent protein (GFP) gene, gfp, of the jellyfish Aequorea victoria is being used as a reporter system for gene expression and as a marker for tracking prokaryotes and eukaryotes. Cells that have been genetically altered with thegfp gene produce a protein that fluoresces when it is excited by UV light. This unique phenotype allowsgfp-tagged cells to be specifically monitored by nondestructive means. In this study we determined whether agfp-tagged strain of Pseudomonas fluorescenscontinued to fluoresce under conditions under which the cells were starved, viable but noncul

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YOON, Hong Mook, Sung Hoon MOON, and Young Hwan SONG. "Characterization of Biosurfactant Produced by Pseudomonas fluorescens PD101." Korean Journal of Fisheries and Aquatic Sciences 36, no. 3 (2003): 230–38. http://dx.doi.org/10.5657/kfas.2003.36.3.230.

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Moynihan, Jennifer A., John P. Morrissey, Eric R. Coppoolse, Willem J. Stiekema, Fergal O'Gara, and E. Fidelma Boyd. "Evolutionary History of the phl Gene Cluster in the Plant-Associated Bacterium Pseudomonas fluorescens." Applied and Environmental Microbiology 75, no. 7 (2009): 2122–31. http://dx.doi.org/10.1128/aem.02052-08.

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ABSTRACT Pseudomonas fluorescens is of agricultural and economic importance as a biological control agent largely because of its plant association and production of secondary metabolites, in particular 2,4-diacetylphloroglucinol (2,4-DAPG). This polyketide, which is encoded by the eight-gene phl cluster, has antimicrobial effects on phytopathogens, promotes amino acid exudation from plant roots, and induces systemic resistance in plants. Despite its importance, 2,4-DAPG production is limited to a subset of P. fluorescens strains. Determination of the evolution of the phl cluster and understand

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Matondang, Christina Oktora, Muklasin, and Loekas Soesanto. "Potensi ekstrak kasar metabolit sekunder yang dihasilkan Trichoderma asperellum dan Pseudomonas fluorescens untuk pengendalian antraknosa pada buah kakao." E-Journal Menara Perkebunan 91, no. 1 (2023): 87–95. http://dx.doi.org/10.22302/iribb.jur.mp.v91i1.524.

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Cocoa pod anthracnose is an important disease of cocoa and can reduce yields. Many attempts have been made to control anthracnose rot disease on cocoa pods but have not been successful yet. This study aimed to examine the potency of secondary metabolite crude extracts produced by Trichoderma asperellum and Pseudomonas fluorescens solely or in combination in controlling anthracnose rot disease of cocoa pods in the field at Silo Bonto Village, Asahan Regency, North Sumatera Province. The secondary metabolite crude extracts was prepared by the form of conidia or T. asperellum and P. fluorescens c

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R, Vimala, and Suriachandraselvan M. "INFLUENCE OF ANTAGONISTIC AGENT, PLANT PRODUCTS AND CHEMICAL AGENTS ON THE POWDERY MILDEW DISEASE OF BHENDI AND ITS PRODUCTION." Journal of Biopesticides 1, no. 2 (2008): 130–33. http://dx.doi.org/10.57182/jbiopestic.1.2.130-133.

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Field investigations were made to study the influence of integrated disease management involving plant products and biological control agents of powdery mildew of Bhendi (Erysiphe cichoracearum DC) with ten treatments viz.,Pseudomonas fluorescens I18 (0.2%), P.fluorescens 1(0.2%), Ocimum sanctum 10%, Neem Seed Kernel Extract 5%, K2 HPO4 50 mM, Salicylic acid 1mM, O. sanctum 5% + P. fluorescens I18 (0.2%),Neem Seed Kernel Extract 5 % + P.fluorescens I18 (0.2%), Carbendazim 0.1% and Control. Two sprays were given; first one on 30 days after sowing and the second one on 60 days after sowing. Powd

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Sona, B. Warrier and Prashant S. Kharkar*. "DESIGN, SYNTHESIS AND CHARACTERIZATION OF FLUORESCENT PROBES BASED ON SCHIFF BASES." Indo American Journal of Pharmaceutical Sciences 04, no. 06 (2017): 1505–10. https://doi.org/10.5281/zenodo.815365.

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Abstract: Schiff bases of fluorescein were synthesized from fluorescein hydrazide using different aromatic aldehydes. These compounds show bright fluorescence different from that of starting material, fluorescein. The compounds were characterised by spectroscopic methods. Absorption and fluorescence spectra of the compounds were also recorded. Keywords: Fluorescein, Schiff bases, Fluorescein hydrazide, fluorescent probes, fluorescence

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Ibrar Wali, Syeda Asma Bano, Sobia Nisa, et al. "Isolation and Identification of Bacillus Subtilis and Pseudomonas Fluorescens from Wheat Rhizosphere and Their Use as Biocontrol Agents." Indus Journal of Bioscience Research 2, no. 2 (2024): 918–31. https://doi.org/10.70749/ijbr.v2i02.295.

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Some bacteria may be used as biocontrol agents against fungal pathogens. Biocontrol agents are environment friendly and cost effective for controlling different plant pathogens. Fungal plant pathogens cause detrimental effects on plants causing diseases and yield loss. The bacterial strains Pseudomonas fluorescens and Bacillus subtilis live abundantly in rhizospheric soil and have antagonistic activity against other organisms. The objective of present study was to isolate and identify the Pseudomonas fluorescens and Bacillus subtilis from rhizospheric soil of Triticum aestivum and their use as

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FARRAG, SEHAM A., and ELMER H. MARTH. "Growth of Listeria monocytogenes in the Presence of Pseudomonas fluorescens at 7 or 13°C in Skim Milk." Journal of Food Protection 52, no. 12 (1989): 852–55. http://dx.doi.org/10.4315/0362-028x-52.12.852.

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Autoclaved samples of skim milk were inoculated with Listeria monocytogenes (strain Scott A, California or V7), Pseudomonas fluorescens (strain P26 or B52), or a combination of L. monocytogenes plus P. fluorescens, and incubated at 7 or 13°C for 8 weeks. McBride Listeria Agar was used to determine populations of L. monocytogenes (at 0, 7, 14, 28, 42, or 56 d), and Pseudomonas isolation agar to enumerate P. fluorescens. Growth of L. monocytogenes was somewhat enhanced after 7 d of incubation at 7 but not at 13°C in the presence of pseudomonads. However, after 14 d and until the end of the incub

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Ulhaq, Muhamad Aditia, and Rachmi Masnilah. "Pengaruh Penggunaan Beberapa Varietas dan Aplikasi Pseudomonas fluorescens untuk Mengendalikan Penyakit Bulai (Peronosclerospora maydis) pada Tanaman Jagung (Zea mays L.)." Jurnal Pengendalian Hayati 2, no. 1 (2019): 1. http://dx.doi.org/10.19184/jph.v2i1.17131.

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Corn is one of the commodities that have high economic value and important role in meeting food needs in Indonesia. Unfavorable conditions on the rate of the higher demand for corn. Pests to be one limiting factor that causes a decrease in the production of corn. Peronosclerospora maydis is a pathogen that causes downy mildew on corn. P. maydis infects corn plants at the age of 2-3 weeks, with the level of damage reaches 80-100%. Control efforts against this disease one of them using antagonistic microbes such as bacteria Pseudomonas fluorescens. P. fluorescens has the potential to control dow

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Gandraburova, N. I., E. I. Kharina, and A. G. Gadzhiahmedova. "EFFECTS OF PSEUDOMONAS FLUORESCENS ON SOME SOIL MICROORGANISMS." Nauka v sovremennom mire 37, no. 4 (2019): 13–17. http://dx.doi.org/10.31618/2524-0935-2019-37-4-13-17.

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Bellassi, Paolo, Gabriele Rocchetti, Lorenzo Morelli, Biancamaria Senizza, Luigi Lucini, and Fabrizio Cappa. "A Milk Foodomics Investigation into the Effect of Pseudomonas fluorescens Growth under Cold Chain Conditions." Foods 10, no. 6 (2021): 1173. http://dx.doi.org/10.3390/foods10061173.

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Pseudomonas fluorescens is a psychrotrophic species associated with milk spoilage because of its lipolytic and proteolytic activities. Consequently, monitoring P. fluorescens or its antecedent activity in milk is critical to preventing quality defects of the product and minimizing food waste. Therefore, in this study, untargeted metabolomics and peptidomics were used to identify the changes in milk related to P. fluorescens activity by simulating the low-temperature conditions usually found in milk during the cold chain. Both unsupervised and supervised multivariate statistical approaches show

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CHAN, WENDY K. M., SEON-TEA JOO, CAMERON FAUSTMAN, QUN SUN, and ROBERT VIETH. "Effect of Pseudomonas fluorescens on Beef Discoloration and Oxymyoglobin Oxidation In Vitro†." Journal of Food Protection 61, no. 10 (1998): 1341–46. http://dx.doi.org/10.4315/0362-028x-61.10.1341.

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The relationship between bacterial growth and oxymyoglobin oxidation in vitro and in meat was studied. In the in vitro study, oxymyoglobin was combined with Pseudomonas fluorescens or sterile nutrient broth (control) in an airtight vessel. P. fluorescens samples showed greater metmyoglobin formation and oxygen consumption than controls. The P. fluorescens population in the reaction vessels was correlated with metmyoglobin formation (r = 0.85, P &lt; 0.05) and oxygen consumption (r = 0.91, P &lt; 0.05). When P. fluorescens and oxymyoglobin were combined in an airtight vessel, reducing t

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Mudi, La, Andi Bahrun, and Gusti Ayu Kade Sutariati. "Bio-Priming Benih Menggunakan Campuran Rizobakter Indigenous untuk meningkatkan Kualitas Fisiologis Benih Kedelai (Glycine max L. Merril)." Berkala Penelitian Agronomi 5, no. 2 (2019): 1. http://dx.doi.org/10.33772/bpa.v6i1.7508.

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Penelitian ini bertujuan untuk mempelajari efektivitas bio-priming benih menggunakan campuaran rizobakter indigenous untuk meningkatkan kualitas fisiologis benih kedelai. Penelitian telah dilakukan pada Bulan November 2014 sampai dengan Januari 2015 di Laboratorium Agroteknologi Fakultas Pertanian Universitas Halu Oleo. Penelitian ini dilaksanakan menggunakan Rancangan Acak Lengkap (RAL) factor tunggal yang terdiri dari delapan perlakuan yaitu: control (tanpa perlakuan rizobakter), bio-priming menggunakan Bacillus sp. CKD061, bio-priming menggunakan P. fluorescens PG01, bio-priming menggunakan

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Gangwar, Gokil Prasad, and A. P. Sinha. "Effect of fungal and bacterial bioagent application on total phenolic content in rice leaves pre-inoculated with Xanthomonas oryzae pv. oryzae (Uyeda and Ishiyama) Dowson." Journal of Applied and Natural Science 6, no. 1 (2014): 254–57. http://dx.doi.org/10.31018/jans.v6i1.410.

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Present study was carried out to observe the effect of fungal and bacterial bioagents on total phenolic content in rice leaves pre-inoculated with Xanthomonas oryzae pv. oryzae and on disease severity of bacterial leaf blight of rice. Two commercial formulations of Trichoderma harzianum (PBA-1) and Pseudomonas fluorescens (PBA-2) and four formulations of fluorescent pseudomonads and Trichoderma spp. viz, P. fluorescens (Pf 83, rice leaf isolate), fluorescent pseudomonad (FLP 88, rice leaf isolate), T. harzianum (rice leaf isolate), Trichoderma spp. (isolate 40, isolated from rice field soil) w

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Kumar Maurya, Manoj, Ramji Singh, and Ajay Tomer. "IN VITRO EVALUATION OF ANTAGONISTIC ACTIVITY OF PSEUDOMONAS FLUORESCENSAGAINST FUNGAL PATHOGEN." Journal of Biopesticides 07, no. 01 (2014): 43–46. http://dx.doi.org/10.57182/jbiopestic.7.1.43-46.

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ABSTRACT The present investigation was undertaken to isolate different strains of Pseudomonas fluorescens from various agroecological zones or crop’s rhizosphere like moong, brinjal, rice, chilli, mustard, chirchida and tomato. Totally eight micro flora resembling Pseudomonas fluorescens were isolatedand three isolates were confirmed as P. fluorescens (strain P.f.01, strain P.f.05 and strain P.f.07). Pseudomonas fluorescens strains P.f 07 were found most effective with the highest antagonisticactivity against three fungal pathogen and show maximum inhibition of mycelial growth of Fusariummonil

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Elita, Nelson, Rita Erlinda, Yefriwati Yefriwati, Deliana Andam Sari, and Ayu Kurnia Illahi. "Peningkatan Kualitas Pupuk Hayati Diperkaya dengan Bakteri Pelarut Kalium, Fosfor dan Penambat Nitrogen Indigenous dari Berbagai Rizosfer Tanaman Padi Terhadap Kandungan Hara dan Jumlah Populasi Mikroba." Agroteknika 7, no. 4 (2024): 655–71. https://doi.org/10.55043/agroteknika.v7i4.418.

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Pupuk hayati merupakan pupuk organik mengandung sekelompok mikroorganisme yang beragam, berperan mendorong pertumbuhan tanaman dan menjaga kesehatan tanah. Penelitian sebelumnya pupuk hayati dengan penambahan bakteri Azotobacter sp, Pseudomonas fluorescens, Bacillus thuringiensis. Kebaharuan penelitian ini adalah pupuk hayati diperkaya dengan bakteri pelarut kalium berasal dari berbagai rizosfer tanaman padi. Tujuan penelitian memperoleh jenis bakteri pelarut kalium efektif meningkatkan kandungan hara pupuk hayati. Penelitian dilaksanakan dengan menggunakan rancangan acak lengkap dengan 8 perl

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Hameed, Hussein M., Faiq H. Ali, and Maytham A. Makki. "The Effect of Pseudomonas fluorescens and Salicylic Acid, and their Synergistic Effect on Protein, Carbohydrates Content and Leaf Area of Two Varieties of Triticum aestivum L. Plant." INTERNATIONAL JOURNAL OF DRUG DELIVERY TECHNOLOGY 12, no. 02 (2022): 662–65. http://dx.doi.org/10.25258/ijddt.12.2.34.

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The study aims to show the effect of salicylic acid at concertation (50–100 and 150 ppm) and Pseudomonas fluorescens at (105,106,107 cfu/mL) on protein, carbohydrates content and leaf area for two varieties (Abu-Ghraib, Abaa 99) of Triticum aestivum L. The results are indicated the significant effect of P. fluorescens and salicylic acid (SA) at the combination of concentration of P. fluorescens (FP2) and SA (SA2) for variety (Abu-Ghraib) was more impact than (Abaa 99) on the physiological aspect like protein, carbohydrates content and leaf area (7.427, 55.55, 26.42) mg/g dry weight respectivel

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