Relevant bibliographies by topics / Fluorescents probes

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Fluorescents probes'

Author: Grafiati
Published: 4 June 2021
Last updated: 10 February 2022

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Journal articles on the topic "Fluorescents probes"

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Zhang, Yujin, and Wei Hu. "Sensing Performance and Efficiency of Two Energy Transfer-Based Two-Photon Fluorescent Probes for pH." Sensors 18, no. 12 (December 13, 2018): 4407. http://dx.doi.org/10.3390/s18124407.

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The design and synthesis of fluorescent probes for monitoring pH values inside living cells have attracted great attention, due to the important role pH plays in many biological processes. In this study, the optical properties of two different two-photon fluorescent probes for pH are studied. The ratiometric sensing of the probes are theoretically illustrated. Meanwhile, the recognitional mechanisms of the probes are investigated, which shows the energy transfer process when react with H+. Specially, the calculated results demonstrate that Probe1 possesses a higher energy transfer efficiency and a larger two-photon absorption cross-section than Probe2, indicating it to be a preferable pH fluorescent probe. Therefore, the influence of connection between the donor and the acceptor on the sensing performances of the probe is demonstrated. Our results help to understand the experimental observations and provide a theoretical basis to synthesize efficient two-photon fluorescent probes for monitoring pH changes.
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Yan, Yuling, M. Emma Marriott, Chutima Petchprayoon, and Gerard Marriott. "Optical switch probes and optical lock-in detection (OLID) imaging microscopy: high-contrast fluorescence imaging within living systems." Biochemical Journal 433, no. 3 (January 14, 2011): 411–22. http://dx.doi.org/10.1042/bj20100992.

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Few to single molecule imaging of fluorescent probe molecules can provide information on the distribution, dynamics, interactions and activity of specific fluorescently tagged proteins during cellular processes. Unfortunately, these imaging studies are made challenging in living cells because of fluorescence signals from endogenous cofactors. Moreover, related background signals within multi-cell systems and intact tissue are even higher and reduce signal contrast even for ensemble populations of probe molecules. High-contrast optical imaging within high-background environments will therefore require new ideas on the design of fluorescence probes, and the way their fluorescence signals are generated and analysed to form an image. To this end, in the present review we describe recent studies on a new family of fluorescent probe called optical switches, with descriptions of the mechanisms that underlie their ability to undergo rapid and reversible transitions between two distinct states. Optical manipulation of the fluorescent and non-fluorescent states of an optical switch probe generates a modulated fluorescence signal that can be isolated from a larger unmodulated background by using OLID (optical lock-in detection) techniques. The present review concludes with a discussion on select applications of synthetic and genetically encoded optical switch probes and OLID microscopy for high-contrast imaging of specific proteins and membrane structures within living systems.
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Mei, Qunbo, Ruqiang Tian, Yujie Shi, Qingfang Hua, Chen Chen, and Bihai Tong. "A series of selective and sensitive fluorescent sensors based on a thiophen-2-yl-benzothiazole unit for Hg2+." New Journal of Chemistry 40, no. 3 (2016): 2333–42. http://dx.doi.org/10.1039/c5nj02259b.

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Different types of fluorescent probes for Hg2+ based on the 5-thiophen-2-yl-benzothiazole derivatives (TBT, CTBT, DTBT and NTBT) were realized by changing the subsituents, including the fluorescence quenching probe, the fluorescence enhancement probe and the ratiometric fluorescent probe.
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Zhou, Dan, Lei Zhou, Xiaoyan Hu, Yan Hu, and Ping Hu. "Detection and Imaging Application of miRNA in Cells and Living Organisms with Nano-Fluorescent Probes Made by Novel Synthesis Materials." Science of Advanced Materials 11, no. 12 (December 1, 2019): 1806–15. http://dx.doi.org/10.1166/sam.2019.3724.

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As a kind of rare earth fluorescent material, the rare earth upconversion nanomaterial can be applied in various fields such as biological detection and imaging, solar cells, and safe positioning, which has attracted wide concerns. In this study, the novel material is applied to the preparation of biological nano-fluorescent probes. Due to its broad UV absorption spectrum, cobalt oxyhydroxide is selected and used as a quencher for upconversion nanomaterials. Once the cobalt oxyhydroxide is placed on upconversion nanomaterials, the surface reaction can effectively remove the fluorescence reaction of the upconversion nanomaterial. In terms of the molecular miRNA tests for cells and living organisms, the nano-fluorescent probe can reduce the fluorescence intensity of miRNA, while the control group can finish the normal fluorescence reaction. The designed fluorescent probe can adjust the contents of cobalt oxyhydroxides and cells to regulate the fluorescence intensity. In terms of the miRNA sensitivity tests, the fluorescence intensity detected by the nano-fluorescent probe is significantly lower than that in the control group, which can be observed through the fluorescence recovery tests of the chemical system. After the addition of miRNA obtained from cells or living organisms, the fluorescent probe has apparently changed the fluorescence intensity of miRNA in cells/living organisms. Also, the detection range of miRNA is effectively expanded, i.e., the different concentrations of miRNA can be detected by adjusting the ratio of the components of the fluorescent probes, which indicates the excellent sensitivity of the fluorescent probe in detecting miRNA in cells and living organisms. In terms of the miRNA tests for cells, different degrees of cancer cells are selected. The fluorescent probe can discriminate the concentration of cancer cells according to fluorescence imaging of cancer cells, thereby further explaining that the fluorescent probe has high-sensitivity in bio-detection.
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Le, X. Chris, Victor Pavski, and Hailin Wang. "2002 W.A.E. McBryde Award Lecture — Affinity recognition, capillary electrophoresis, and laser-induced fluorescence polarization for ultrasensitive bioanalysis." Canadian Journal of Chemistry 83, no. 3 (March 1, 2005): 185–94. http://dx.doi.org/10.1139/v04-175.

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The combination of affinity recognition, capillary electrophoresis (CE), laser-induced fluorescence (LIF), and fluorescence polarization for the ultrasensitive determination of compounds of biological interest is described. Competitive immunoassays using CE–LIF eliminate the need for fluorescently labeling trace analytes of interest and are particularly useful for determination of small molecules, such as cyclosporine, gentamicin, vancomycin, and digoxin. Fluorescence polarization allows for differentiation of the antibody-bound from the unbound small molecules. Noncompetitive affinity CE–LIF assays are shown to be highly effective in the determination of biomarkers for DNA damage and HIV-1 infection. An antibody (or aptamer) is used as a fluorescent probe to bind with a target DNA adduct (or the reverse transcriptase of the HIV-1 virus), with the fluorescent reaction products being separated by CE and detected by LIF. Aptamers are attractive affinity probes for protein analysis because of high affinity, high specificity, and the potential for a wide range of target proteins. Fluorescence polarization provides unique information for studying molecular interactions. Innovative integrations of these technologies will have broad applications ranging from cancer research, to biomedical diagnosis, to pharmaceutical and environmental analyses.Key words: capillary electrophoresis, laser-induced fluorescence, fluorescence polarization, immunoassay, affinity probes, antibodies, aptamers, DNA damage, toxins, therapeutic drugs.
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Qin, Qiu Yan, Yi Ping Qian, Zi Yu Wang, and Xiao Lin Fan. "Design and Synthesis of Fluorescent Betahistine Conjugates with Unique Imaging Property." Advanced Materials Research 557-559 (July 2012): 712–15. http://dx.doi.org/10.4028/www.scientific.net/amr.557-559.712.

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Labeling cardiovascular drugs probes with a fluorescent tag is an alternative method of measuring drugs activities and distributions in vivo, and further using of advanced tools to diagnose or detect cardiovascular diseases. Using this approach, a fluorescent probe (betahistine-Flu, 1) of Betahistine-based was synthesized and characterized by 1H NMR, 13C NMR and LC-MS, and its UV-Vis absorption spectral and fluorescence spectral, and fluorescence imaging in cell model were investigated. It was found that the fluorescent probe display strong green fluorescence, and have good optical effect in cell. This study reveals a good and interesting results of betahistine-directed fluorescent probe, and its may be a possible candidate for cardiovascular disease diagnosis and analysis in vivo.
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Kircher, Moritz F., Lee Josephson, and Ralph Weissleder. "Ratio Imaging of Enzyme Activity Using Dual Wavelength Optical Reporters." Molecular Imaging 1, no. 2 (April 1, 2002): 153535002002011. http://dx.doi.org/10.1162/15353500200201124.

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The design of near-infrared fluorescent (NIRF) probes that are activated by specific proteases has, for the first time, allowed enzyme activity to be imaged in vivo. In the current study, we report on a method of imaging enzyme activity using two fluorescent probes that, together, provide improved quantitation of enzymatic activity. The method employs two chemically similar probes that differ in their degradability by cathepsin B. One probe consists of the NIRF dye Cy5.5 attached to a particulate carrier, a crosslinked iron oxide nanoparticle (CLIO), through cathepsin B cleavable l-arginyl peptides. A second probe consists of Cy3.5 attached to a CLIO through proteolytically resistant d-arginyl peptides. Using mixtures of the two probes, we have shown that the ratio of Cy5.5 to Cy3.5 fluorescence can be used to determine levels of cathepsin B in the environment of nanoparticles with macrophages in suspension. After intravenous injection, tissue fluorescence from the nondegradable Cy3.5–d-arginyl probe reflected nanoparticle accumulation, while fluorescence of the Cy5.5–l-arginyl probe was dependent on both accumulation and activation by cathepsin B. Dual wavelength ratio imaging can be used for the quantitative imaging of a variety of enzymes in clinically important settings, while the magnetic properties of the probes allow their detection by MR imaging.
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Song, Meng Meng, Wen Juan Guo, Zhao Dai, Kai Li Qiu, and Jun Fu Wei. "Fluorescent DNA Probes Based on Quantum Dots and Core/Shell Quantum Dots." Applied Mechanics and Materials 372 (August 2013): 115–18. http://dx.doi.org/10.4028/www.scientific.net/amm.372.115.

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Fluorescent DNA probes based on the fluorescence resonance energy transfer (FRET) were presented in this paper when AuNPs were as the energy acceptors and CdTe quantum dots and CdTe/SiO2 core/shell nanoparticles were as the energy acceptors, respectively. The DNA probes were prepared when energy donors and acceptors were conjugated with two single-stranded complementary oligonucleotides and hybridized with each other and the fluorescent intensity of probes could be decreased. The quenching efficiency of DNA probe was about 67 % when CdTe QDs were as the energy donors, while that of DNA probe was about 75 % when CdTe/SiO2 fluorescent core/shell nanoparticles were as the energy acceptors, which indicated that CdTe/SiO2 core/shell nanoparticles were suitable donors compared with CdTe QDs in DNA probe field.
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Wagner, Brian D., Amy E. Arnold, Spencer T. Gallant, Carmen R. Grinton, Julia K. Locke, Natasha D. Mills, Carrie A. Snow, Timara B. Uhlig, and Christen N. Vessey. "The polarity sensitivity factor of some fluorescent probe molecules used for studying supramolecular systems and other heterogeneous environments." Canadian Journal of Chemistry 96, no. 7 (July 2018): 629–35. http://dx.doi.org/10.1139/cjc-2017-0727.

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Fluorescence spectroscopy provides an excellent technique for investigating heterogeneous systems, due to its high sensitivity and the large effect of the local environment on molecular emission. In addition, the use of polarity-sensitive fluorescent probes as guests in supramolecular host–guest inclusion complexes can be exploited in fluorescent sensors. This paper identifies, tabulates, and quantifies a series of useful polarity-sensitive fluorescent probes, with a wide range of polarity-dependent fluorescence responses. The degree of polarity sensitivity is quantified using the polarity sensitivity factor (PSF), developed in our laboratory. In most cases, such polarity-sensitive probes show increased emission as the local polarity is decreased (PSF > 1); 10 such probes are described. However, less commonly, “reverse polarity dependence” can occur in which probe emission decreases with decreasing polarity (PSF < 1); four such probes are described. The mechanism for the observed polarity-induced fluorescence changes will also be discussed in selected representative cases. The purpose of this paper is to present details on a broad arsenal of polarity-sensitive fluorescence probes with varying properties, with potentially useful applications in the study of heterogeneous systems, including inclusion phenomena, and in practical applications such as fluorescent sensors, which will be useful to researchers studying supramolecular and other heterogeneous systems using fluorescence spectroscopy.
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Park, Sun Young, Eugeine Jung, Jong Seung Kim, Sung-Gil Chi, and Min Hee Lee. "Cancer-Specific hNQO1-Responsive Biocompatible Naphthalimides Providing a Rapid Fluorescent Turn-On with an Enhanced Enzyme Affinity." Sensors 20, no. 1 (December 20, 2019): 53. http://dx.doi.org/10.3390/s20010053.

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Human NAD(P)H:quinone oxidoreductase 1 (hNQO1) is overexpressed in cancer cells and associated with the drug resistance factor of cancer. The objective of this work is the development of fluorescent probes for the efficient detection of hNQO1 activity in cancer cells, which can be employed for the cancer diagnosis and therapeutic agent development. Herein, we report naphthalimide-based fluorescent probes 1 and 2 that can detect hNQO1. For hNQO1 activity, the probes showed a significant fluorescence increase at 540 nm. In addition, probe 1, the naphthalimide containing a triphenylphosphonium salt, showed an enhanced enzyme efficiency and rapid detection under a physiological condition. The detection ability of probe 1 was superior to that of other previously reported probes. Moreover, probe 1 was less cytotoxic during the cancer cell imaging and readily provided a strong fluorescence in hNQO1-overexpressed cancer cells (A549). We proposed that probe 1 can be used to detect hNQO1 expression in live cells and it will be applied to develop the diagnosis and customized treatment of hNQO1-related disease.
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Dissertations / Theses on the topic "Fluorescents probes"

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Ardizzone, Antonio. "New fluorescent nanovesicles, by self-assembly of organic fluorophores, sterols and surfactants, as probes for bioimaging." Doctoral thesis, Universitat Autònoma de Barcelona, 2017. http://hdl.handle.net/10803/403924.

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El estudio de nuevas nanopartículas orgánicas fluorescentes (FONs) que puedan superar los límites de las comunes sondas fluorescentes como fluoróforos moleculares, proteínas fluorescentes y nanopartículas inorgánicas es un tema de gran interés para los científicos de materiales que desarrollan nuevas sondas para microscopía de fluorescencia y teranóstica. En los últimos años, se han desarrollado nuevas nanovesículas no liposómicas, basadas en el autoensamblaje de tensioactivos y esteroles, denominados Quatsomes (QSs), que constituyen unos prometedores vehículos de fármacos multifuncionales. Dentro de este escenario, el objetivo principal de esta Tesis (realizada en el marco del proyecto Marie Skłodowska-Curie ITN “Nano2Fun”) es explorar la posibilidad de utilizar los Quatsomes como vehículos para la nano-estructuración en medios acuosos de varias moléculas fluorescentes, independientemente de sus propiedades físico-químicas y ópticas, con el fin de obtener nuevas nanopartículas orgánicas fluorescentes (FONs) con elevada estabilidad coloidal y características fluorescentes optimales, especialmente en relación al brillo. El auto-ensamblaje de fluoróforos orgánicos moleculares, esteroles y tensioactivos de amonio cuaternario en Quatsomes fluorescentes se logró mediante el método DELOS-SUSP, un proceso a base de CO2 comprimido, que garantiza una composición de membrana y una disposición supramolecular altamente homogéneas y, como consecuencia, nanovesículas con elevadas propiedades ópticas. Se han explorado diferentes estrategias para la nano-estructuración en medios acuosos, por medio de QSs, de moléculas fluorescentes con diferentes propiedades fisicoquímicas, incluyendo aquellas solubles y no solubles en agua, analizando el impacto de la nano-estructuración sobre las propiedades ópticas de las FONs obtenidas. De esta manera, los fluoroforos aniónicos solubles en agua, como la fluoresceína, fueron nano-estructurados encima de los QSs. Por otra parte, los fluoróforos lipófilos y no solubles en agua, modificados con largas cadenas alquílicas pueden incorporarse de forma estable en la membrana de los QSs, como se muestra en el caso de varias familias de colorantes, por ejemplo, las cianinas, los diketopirrolopirroles (DPP) y los derivados del fluorene. Los QSs fluorescentes mostraron una estabilidad óptica coloidal excepcional (hasta varios meses), un alto grado de homogeneidad estructural y altas eficiencias de fluorescencia, mostrando mejores prestaciones en comparación con otras nanoestructuras de los mismos fluoroforos. Además, con el objetivo de obtener nanopartículas multicolores, los Quatsomes permitieron cargar simultáneamente diferentes fluoroforos dentro de sus membranas, mostrando un mecanismo de transferencia de energía de resonancia de fluorescencia (FRET) altamente eficiente, una interesante herramienta para monitorear la integridad del carrier durante la administración del fármaco y para la adquisición de imágenes multicolores. En conclusión, los Quatsomes fluorescentes se probaron como nano-sondas para la obtención de imágenes de células in vitro. Se ha demostrado que los Quatsomes que incorporan derivados del fluorene (denominados LysoQS) constituyen una sonda lisosómica altamente específica, ideal para la adquisición de imágenes en tiempos largos. Además, los Quatsomes cargados de cianinas se utilizaron como sondas para técnicas de microscopía superresolución (STORM), que permitió la visualización y resolución de Quatsomes individuales tras la internalización en las células. Los resultados de esta tesis muestran que los Quatsomes fluorescentes, gracias a las ventajas ofrecidas en comparación con otros marcadores fluorescentes comúnmente empleados, son unas nanosondas altamente prometedoras, con posibles aplicaciones futuras en bioimagen, teragnostica y, en general, nanomedicina.
Finding new fluorescent organic nanoparticles (FONs) with the potential to overcome the limits of common fluorescent probes as molecular fluorophores, fluorescent proteins and inorganic nanoparticles is a subject of strong interest for materials scientists developing new probes for fluorescence microscopy and theranostics. In the recent years, innovative non-liposomal nanovesicles, based on the self-assembly of quaternary ammonium surfactants and sterols, named Quatsomes (QSs), have been developed as promising candidates for applications as multifunctional drug carriers. Within this scenario, the main objective of this Thesis (conducted in the framework of the Marie Skłodowska-Curie ITN “Nano2Fun”) is to explore the possibility of using Quatsomes as a vehicle for nanostructuring in aqueous media several dye molecules, irrespective of their physicochemical and optical properties, in order to obtain new fluorescent organic nanoparticles (FONs) with superior colloidal stability and enhanced fluorescent features, especially with high brightness, in relation to single molecule flurofores and other type of FONS. The self-assembly of molecular organic fluorophores, sterols and quaternary ammonium surfactants into fluorescent Quatsomes was achieved by the DELOS-SUSP method, a compressed CO2 –based process which guarantees a highly homogeneous membrane composition and supramolecular arrangement, which have impact on the optical properties of the obtained FONs. Different strategies have been explored to nanostructurate in aqueous media, by mean of QSs, molecular dyes with different physicochemical properties, including those water- and non-water soluble, analyzing the impact of their nanostructuration on the optical properties of the obtained FONs. Thus, anionic water-soluble dyes, such as fluorescein, were nanostructured over QSs surface, taking advantage of anionic/cationic interaction among dye and vesicles surface. On the other hand, lipophilic and non-water soluble dyes modified with long alkyl chains can be stably incorporated into QSs membrane, as shown in the case of several dyes families, including cyanine, diketopyroolopyrrole (DPPs) and fluorene derivatives. The fluorescent QSs showed superior colloidal and optical stability (up to several months), a high degree of structural homogeneity and high fluorescence performances, overcoming those of other nanostructures of the same dyes. Furthermore, aiming to obtain multicolor nanoparticles, Quatsomes allowed the simultaneous loading within their membrane of different dyes, which showed a highly efficient fluorescence resonance energy transfer (FRET) mechanism, an interesting tool for monitoring the carrier integrity during the drug delivery and for multiplexed imaging applications. Finally, fluorescent Quatsomes were tested as nanoprobes for in vitro cells imaging. It has been demonstrated that fluorene-based Quatsomes (named LysoQS) constitute a strongly specific lysosomal probe ideal for long-term imaging. Furthermore, cyanines-loaded Quatsomes were used as probes for super-resolution microscopy technique (STORM) which allowed visualizing and resolving single Quatsomes structures upon internalization in cells. The results of this Thesis showed that fluorescent Quatsomes, thanks to the advantages offered in comparison with other commonly employed fluorescent labels, constitute a promising fluorescent nanoprobes with possible future applications in bioimaging, theranostics and, generally, nanomedicine.
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Celeghini, Eneiva Carla Carvalho. "Efeitos da criopreservação do sêmen bovino sobre as membranas plasmática, acrossomal e mitocondrial e estrutura da cromatina dos espermatozóides utilizando sondas fluorescentes." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/10/10131/tde-21092006-111358/.

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A criopreservação, como já conhecido, causa danos da função celular podendo, com isso, apresentar redução da fertilidade. O desenvolvimento de técnicas para avaliar a viabilidade espermática, que visam aperfeiçoar o processo de avaliação seminal tem sido meta de muitas pesquisas. Este trabalho foi dividido em dois experimentos. O experimento 1 foi realizado para o desenvolvimento e validação de técnicas práticas e de alta repetibilidade para a avaliação simultânea da integridade das membranas plasmática e acrossomal e função mitocondrial de espermatozóides bovinos pela associação de sondas fluorescentes. O experimento 2 foi realizado para avaliar os efeitos que a criopreservação pode causar sobre a motilidade espermática, membranas plasmática, acrossomal e mitocondrial (pela técnica desenvolvida no experimento 1) e estrutura da cromatina de espermatozóides bovinos frente a dois diluidores diferentes, bem como verificar a existência de correlações entre alterações nas membranas e características de motilidade em espermatozóides bovinos criopreservados. No experimento 1 as associações das sondas iodeto de propídio (PI, para avaliar integridade da membrana plasmática) e aglutinina de Pisum sativum conjugada a isotiocionato de fluoresceína (FITC-PSA, para avaliar a integridade acrossomal), foram divididas em quatro protocolos, nos quais foram testadas diferentes sondas para avaliar a função mitocondrial. Rodamina 123 (R123, protocolo 1), MitoTracker Green FM (MITO, protocolo 2), CMXRos ou MitoTracker red (protocolo 3) e no: iodeto de 5,5’,6,6’-tetracloro-1,1’,3,3’-tetraetilbenzimidazolilcarbocianina (JC-1, protocolo 4). Estes protocolos foram testados e validados. Os resultados obtidos foram submetidos à análise de variância, teste de Fisher e à análise de regressão linear pelo programa Stat-View® (SAS Institute, Inc. 1998). O protocolo 1 não apresentou resultados satisfatórios, não sendo possível validar, enquanto, os protocolos 2, 3 e 4 foram validados e apresentaram resultados bastante consistentes. O protocolo 4 foi escolhido para a utilização no experimento 2. Para o experimento 2 foram realizadas sete colheitas de sêmen de oito touros puros da raça Simental. Após a colheita, o sêmen foi avaliado quanto ao volume, concentração, motilidade e vigor visualmente, motilidade por sistema computadorizado (CASA), características morfológicas por microscopia de interferência diferencial, integridade das membranas plasmática e acrossomal, função mitocondrial, pela associação de FITC-PSA, PI e JC-1, e integridade da estrutura da cromatina, pela técnica da acridina laranja. Após as análises, o sêmen foi dividido em duas alíquotas, sendo uma das alíquotas diluída com o diluidor Bioxcell® e a outra com o diluidor Botu-Bov®, envasado em palhetas e submetido a congelação com um sistema automatizado. Duas palhetas cada tratamento foram descongeladas e o sêmen foi avaliado quanto a motilidade e vigor visualmente, motilidade pelo CASA, características morfológicas, integridade das membranas plasmática e acrossomal, função mitocondrial e estrutura da cromatina. A análise estatística foi realizada utilizando-se o programa Statistical Analysis System (SAS Institute Inc., 1985), os dados foram submetidos à análise de variância e teste de Tukey. Foram calculadas correlações lineares de Pearson. O nível de significância foi fixado em 5%. Foram observados efeitos da criopreservação e do diluidor sobre motilidade e vigor visual, motilidade pelo CASA, características morfológicas, integridade das membranas plasmática e acrossomal e função mitocondrial. De forma que, após a criopreservação houve aumento dos danos nas estruturas espermática e o diluidor Botu-Bov® apresentou a maioria das características melhores quando comparado ao diluidor Bioxcell®
The process of cryopreservation causes damages in the sperm cellular function which may lead to a reduction in fertility. New techniques to evaluate sperm viability may improve the process of semen evaluation. The present work was divided in two experiments. Experiment 1 aimed the development and validation of practical techniques with high repetibility for simultaneous evaluation of integrity of plasmatic and acrosomal membrane and mitochondrial function of bovine spermatozoa by the strategic association of fluorescent probes. Objective of experiment 2 was to validate the effects of cryopreservation using two differents diluents on sperm motility, plasmatic, acrosomal, mitochondrial membranes integrity and chromatin structure of bovine spermatozoa. A second objective was to verify the correlations between alterations in membranes and motility characteristics in cryopreserved bovine spermatozoa. In experiment 1 propidium iodide (PI, to evaluate plasmatic membrane integrity) and the Pisum sativum agglutinin conjugated with fluorescein isothicyonate- (FITC-PSA, to evaluate acrosomal integrity). Probes were associated to evaluate mitochondrial function in four protocols: Rhodamine 123 (R123, Protocol 1), MitoTracker Green FM (MITO, protocol 2), CMXRos or MitoTracker red (protocol 3), and 5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzimidazolyl carbocyanine iodide (JC-1, protocol 4). Results were submitted to analysis of variance, Fisher’s test and linear regression analysis by the program Stat-View® ( SAS Institute, Inc.1998). It was not possible to validate protocol 1, while the protocols 2, 3 and 4 were validated and showed consistent results. Protocol 4 was used in experiment 2. For experiment 2 seven ejaculates were collected from eight Simental bulls. Volume, concentration, motility and vigour of semen were evaluated subjectively, motility by computer system (CASA), morphological characteristics by differential interference microscopy, plasmatic and acrosomal membranes integrity and, mitochondrial function with FITC-PSA, PI and JC-1 association, and chromatin integrity by acridine orange technique. Then, semen was divided in two aliquots. One was diluted with Bioxcell® and the other with Botu-Bov® diluent, packaged in 0.5 mL straws and frozen using na automated system. Two straws of sêmen from each treatment were thawed and the semen parameter evaluated, as described above. The statistical analysis was performed using the Statistical Analysis System program (SAS Institute Inc., 1985). Data were submitted to analysis of variance and Tukey’s test. Pearson’s linear correlations were calculated. Cryopreservation increased damages in spermatic structures. Botu-Bov® diluent was better in preserve the motility and membrane integrity (P⁢0,05) when compared to Bioxcell® diluent
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Peyrard, Lisa. "Cyclotrivératrylènes fluorescents pour la détection d’ammoniums d’intérêt biologique." Thesis, Bordeaux 1, 2012. http://www.theses.fr/2012BOR14700/document.

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Le développement de sondes moléculaires fluorescentes pour le suivi ex vivo de phénomènes biologiques (communication nerveuse, par exemple) est en plein essor. Dans cette optique, des dérivés fluorescents de cyclotrivératrylènes (CTV), cavitands propices à la complexation de petites molécules organiques, ont été synthétisés. La stratégie utilisée consiste à introduire des groupements électro-donneurs et électro-attracteurs conjugués sur chaque unité aromatique du CTV afin d’avoir des systèmes de type « push-pull ». L’extension de la conjugaison entre les groupements électro-attracteurs et électro-donneurs a été envisagée afin d’améliorer les propriétés spectroscopiques des sondes. Des réactions de couplage organométallique de Sonogashira, ainsi que des cycloadditions alcynes-azotures catalysées par le cuivre (CuAAc), ont été conduites sur le squelette CTV dans ce but. La caractérisation spectroscopique des différentes sondes fluorescentes développées a été réalisée en milieu organique et en conditions physiologiques, pour celles dont la solubilité le permettait. L’étude des propriétés de reconnaissance de ces dernières, pour des ammoniums d’intérêt biologique (tels que les neurotransmetteurs, acétylcholine, dopamine et dérivés) en conditions physiologiques, a également été menée par spectrofluorimétrie, ainsi que par d’autres techniques (RMN, calorimétrie). La formation d’assemblages moléculaires en milieu aqueux a également été mise en évidence (expériences de diffusion dynamique de la lumière, microscopie électronique en transmission), pour certaines sondes lors de ce travail
The development of fluorescent probes for the ex vivo detection of biological phenomena (neuronal communication, for example) presents a growing interest. In this context, fluorescent cyclotriveratrylene (CTV) derivatives have been developed, as these cavitands are known to complex small organic molecules. The strategy used to get interesting spectroscopic properties, was to introduce conjugated electron-donating and electron-withdrawing groups on each aromatic unit of the CTV (leading to “push-pull” systems). To improve the spectroscopic properties of the probes, the conjugation between the electron-donating and the electron-withdrawing groups was extended. Hence, the Sonogashira organometalic coupling reaction and the copper catalyzed cycloaddition directly on CTV skeleton were used. The spectroscopic characterization of the new fluorescent probes synthesized was done, in organic solvent but also in physiological conditions when the solubility permits it. The recognition studies for biological ammoniums, such as neurotransmitters (acetylcholine, dopamine, and derivatives) were performed in physiological conditions by spectrofluorimetry but also other methods (like MNR or micro-calorimetry). The formation of molecular assemblies was also observed (by dynamic light scattering and transmission electron microscopy) during this work with some of the probes
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Oger, Samuel. "Analogues fluorescents de l'epicocconone et sondes pour le piégeage de produits naturels azaphiles." Thesis, Normandie, 2017. http://www.theses.fr/2017NORMR035/document.

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L’étude des phénomènes biologiques et notamment du rôle des protéines au sein d’un mécanisme cellulaire est un défi pour les biologistes. L’avènement de la microscopie de fluorescence à excitation biphotonique et des techniques « super-résolutives » a permis l’amélioration des performances des techniques de microscopie classiques et l’application l’imagerie in vivo pour l’analyse des tissus biologiques. Ces techniques requièrent cependant l’emploi de sondes aux propriétés photophysiques optimisées en complément de la spécificité vis-à-vis de la ou des cible(s) biologique(s). L’epicocconone, une molécule naturelle profluorescente de la famille des azaphilones, est employée en protéomique pour la détection des protéines sur gel d’électrophorèse. Ce composé a la faculté de réagir avec les amines des résidus lysine des protéines pour former un adduit covalent énaminone hautement fluorescent dans le proche infrarouge (610 nm) sous irradiation UV (395 nm) ou visible (520 nm). Différents analogues synthétisés au sein du laboratoire ont permis d’étudier la relation structure-fluorescence de ces composés capables de détecter non spécifiquement les protéines du milieu étudié. Afin d’améliorer la spécificité de ces molécules en vue d’applications en imagerie, la synthèse de sondes polyfonctionnelles, via une réaction de cycloaddition 1,3 dipolaire azoture-alcyne catalysée au cuivre, associant un analogue de l’epicocconone à un agent de reconnaissance possédant une affinité particulière pour une cible biologique a été étudiée. La synthèse d’analogues de l’epicocconone optimisés pour l’absorption biphotonique a également été réalisée lors de cette thèse. Les propriétés optiques linéaires et non linéaires de ces composés ont été étudiées afin de sélectionner le meilleur composé pour des applications en imagerie par microscopie de fluorescence à excitation biphotonique. Enfin, la réactivité particulière des azaphilones a servi de point de départ au développement d’une stratégie d’identification et d’isolement de nouvelles molécules naturelles azaphiles grâce à l’utilisation de sondes à produits naturels capables de cibler spécifiquement ce type de composés
Understanding biological processes that involve proteins is a challenge for biologists. Two-photon excitation and super-resolution microscopy have improved drastically bioimaging techniques allowing in vivo deep tissue analysis. However those techniques require the use of optimized and selective fluorescent probes. Epicocconone is a natural profluorescent azaphilone widely used in proteomics for detecting proteins on electrophoresis gels. This compound can react reversibly with primary amines from lysines forming a covalent enaminone adduct that emits near infrared fluorescence light (610 nm) upon UV (395 nm) or visible (520 nm) excitation. Different analogues, that non-selectively bind to proteins, were previously synthesized in order to understand the structure-fluorescence relationship. The synthesis of polyfunctionnal probes was studied using copper-catalyzed azide-alkyne cycloaddition to connect the epicocconone scaffold to a recognition moiety, which can specifically recognise one biological target. New analogues optimized for two-photon absorption were synthesized. Their linear and non-linear optical properties were determined to select the most suitable molecule for two-photon excitation microscopy.In a last part, the particular reactivity of azaphilones was also regarded as a useful strategy for designing probes which could react specifically with azaphilic natural products in order to identify and isolate new ones
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Nina, Diogo Anthony. "Hétérocycles fluorescents pour la détection des protéines carbonylées associées au vieillissement et à l’inflammation." Thesis, Sorbonne université, 2018. http://www.theses.fr/2018SORUS355.

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Les travaux présentés dans ce manuscrit décrivent la synthèse de nouvelles sondes fluorescentes pour le marquage des protéines carbonylées. Dans une première partie, la synthèse de nouvelles molécules fluorescentes à base 1,2,4-triazoles 1,3,5-trisubstitués est décrite à partir des 4H-pyrido[e][1,3]oxazin-4-ones. L’étude photophysique des 1,2,4- triazoles dans plusieurs solvants a permis de mettre en évidence un mécanisme de fluorescence de type ESIPT induisant un large déplacement de Stokes (jusqu’à 250 nm). La deuxième partie de ce manuscrit est consacrée à la synthèse de nouveaux BODIPY avec une fonction hydrazide. Enfin, la dernière partie décrit les applications de ces nouveaux BODIPY en tant que marqueurs des protéines carbonylées, utilisable avec la 2D-Oxi DIGE, la microscopie à fluorescence et la cytométrie en flux
The works presented in this manuscript describe the synthesis of new fluorescent probes for protein labelling. In a first part, the synthesis of new fluorescent molecules 1,2,4-triazoles based 1,3,5-trisubstituted is described from 4H-pyrido[e][1,3]oxazin-4-ones. The photophysical study of 1,2,4-triazoles in several solvents allowed to highlight a fluorescence ESIPT type mechanism leadingto a wide Stokes’shift (close to 250 nm). The second part of this manuscript is dedicated to the synthesis of new BODIPY with a hydrazide function. Finally, the last part describes the applications of these new BODIPY as fluorescent probes for carbonylated proteins labelling, used in 2D - Oxi DIGE, fluorescence microscopy and flow cytometry
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Zhou, Xiaobo. "Design, synthesis and sensing properties of chiral amine-based fluorescent probes." HKBU Institutional Repository, 2012. https://repository.hkbu.edu.hk/etd_ra/1442.

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Brookner, Carrie Kazinoff. "Biological basis of cervical tissue autofluorescence /." Digital version accessible at:, 1999. http://wwwlib.umi.com/cr/utexas/main.

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Karpenko, Iuliia. "Conception, synthesis and evaluation of fluorescent probes and PET radioligands for the oxytocin and vasopressin receptors." Thesis, Strasbourg, 2014. http://www.theses.fr/2014STRAF045/document.

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Les récepteurs de l’ocytocine (OTR) et de la vasopressine (AVPR) sont connus pour être impliqués dans la modulation d’effets centraux complexes. Récemment l’OTR a été proposé comme une cible thérapeutique pour le traitement des troubles du spectre autistique (TSA).Afin de mieux comprendre le rôle de l’OTR et des AVPR dans les TSA, d’éclaircir des nouveaux traits de sa pharmacologie et d’établir des méthodes du criblage sur les récepteurs sauvages, nous avons développé des traceurs pour la tomographie par émission des positons ainsi que des sondes fluorescentes pour la famille OT/AVP des RCPG. Les ligands fluorescents ont été utilisés pour établir un test de liaison TR-FRET pour l’OTR et pour initier le développement du test alternatif sur les récepteurs sauvages. Les radiotraceurs TEP seront bientôt testés chez la souris et chez le singe pour évaluer leurs performances pour la détection des récepteurs de l’ocytocine centraux avant d’envisager des études chez l’Homme
In order to better understand the role of OTR and AVPR in ASD, to reveal new features in its pharmacology and signaling and to establish high-throughput screening method on wild-type G protein-coupled receptors, we developed imaging probes for the oxytocin-vasopressin receptors family, namely radiotracers for positron emission tomography and optical probes for fluorescence detection and imaging. The fluorescent ligands have been used to establish TR-FRET binding assay for OTR and to initiate the development the screening assay for the wild-type oxytocin receptor. The PET radiotracers will be shortly tested in mice and monkeys to evaluate their potency in detecting the central oxytocin receptors
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Meyer, Yves. "Conception et développement de bras réactifs auto-immolables pour la synthèse de sondes pro-fluorescentes : applications à la détection de peptidases dans un contexte in-vivo." Thesis, Rouen, INSA, 2010. http://www.theses.fr/2010ISAM0018.

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L'objectif de cette thèse est la conception et le développement de bras espaceurs auto-immolables originaux situés entre le substrat peptidique et un fluorophore à phénol. Une première partie de ces travaux porte sur le développement de bras réactifs auto-immolables adaptés à la détection d'exopeptidases et à leur application pour la synthèse de sondes destinées à l'imagerie in-vivo de la caspase 3, une enzime impliquée dans le processus apoptotique. La seconde partie de ce travail relate les efforts effectués afin d'étendre l'utilisation des bras réactifs auto-immolables à la détection des endopeptidases, notamment les MMPs, une famille d'enzimes fortement impliquée dans la progression cancéreuse
The aim of this PhD work is the design and development of novel self-immolative species linking a peptide susbstrate to a phenolic fluorophore. A first part was dedicated to the development of self-immolative linkers for exopeptidases detection and their incorporation in caspase 3 probes to stain the apoptotic process. A second part was devoted to the extension of the strategy to endopeptidases, especially MMPs, an enzyme family mainly involved in cancer progression
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Best, Quinn Adams. "XANTHENE AND SILICON ANALOGS OF XANTHENE FLUOROPHORES AS CHEMICAL SENSORS FOR pH AND HYPOCHLOROUS ACID." OpenSIUC, 2013. https://opensiuc.lib.siu.edu/dissertations/662.

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Chemical sensors capable of detecting a specific atom or molecule under various conditions have been utilized in biological and environmental analyses. Fluorescence based sensors are particularly advantageous in these studies because of their high sensitivity, relative ease in handling, and low technical costs. This dissertation focuses on the detection of two analytes, H+ and hypochlorous acid, which are of interest in biology because the presence of abnormal quantities of these analytes may be indicative of disease. We have established a new platform for which sensitive changes in various regions of pH can be detected using fluorescence. The aminomethylrhodamine (AMR) scaffold is highly versatile, i.e. the pH range in which the sensor is active can be tuned by introducing different substituents on the amine moiety. Overall this systematic approach to the design of pH sensitive fluorophores has allowed for a library of compounds that are responsive over a broad range of pH (pH 3 - 10) by simply changing the substituent on the amino group. We report the synthesis and characterization of a silicon analog of rhodamine for the fluorescence based detection of hypochlorous acid. This fluorophore exhibits a 90 nm bathochromic shift in its absorption and emission, relative to its oxygen counterpart. Hypochlorous acid is a biological agent linked to certain diseases. Therefore, the longer wavelength properties of the this far-red fluorescent sensor will be of significant benefit to imaging experiments of this analyte in biological media and tissue due to its spectral proximity of the so called NIR optical window. Furthermore, the novel synthetic methodology of this sensor possesses a key intermediate, which could potentially lead to future fluorescence based sensors. The characterization of a fluorescent probe designed for the detection of hypochlorous acid (HOCl) using a silicon analog of fluorescein (SiF) was also reported. Over a range of pHs, the probe reacts with a stoichiometric amount of HOCl resulting in a mixture of two pH dependent fluorescent species, a SiF disulfide product and a SiF sulfonate product. The unique colorometric properties of the individual SiF fluorophores were utilized to perform simultaneous detection of HOCl and pH. When an excess of HOCl is present, the SiF fluorophores become chlorinated, via an intermediate halohydrin, resulting in a more pH independent and red-shifted fluorophore. Finally, an attempt was made at developing a pH responsive photodynamic therapy agent. This system was designed to target the relatively low extracellular pH found around tumors. A di-bromohydroxymethylrhodamine system was synthesized and the photophysical properties were characterized. This system absorbs weakly under acidic conditions (ca. pH 3), however was shown to be a moderate photosensitizer under acidic conditions.
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Books on the topic "Fluorescents probes"

1

Slavík, Jan, ed. Fluorescence Microscopy and Fluorescent Probes. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6.

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S, Wolfbeis Otto, ed. Fluorescence methods and applications: Spectroscopy, imaging, and probes. Malden, Mass: Blackwell Pub., 2008.

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Wessels, Jurina Margarete. Fluoreszenzspektroskopische Untersuchungen zur Aufnahmekinetik und Lokalisation von Photosensibilisatoren in vitro. Neuherberg: GSF-Forschungszentrum für Umwelt und Gesundheit, 1993.

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Kraayenhof, Ruud, Antonie J. W. G. Visser, and Hans C. Gerritsen, eds. Fluorescence Spectroscopy, Imaging and Probes. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-642-56067-5.

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Sabnis, R. W. Handbook of Fluorescent Dyes and Probes. Hoboken, NJ, USA: John Wiley & Sons, Inc, 2015. http://dx.doi.org/10.1002/9781119007104.

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Vladimir, Didenko V. Fluorescent Energy Transfer Nucleic Acid Probes. New Jersey: Humana Press, 2006. http://dx.doi.org/10.1385/1597450693.

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Johnson, Iain D., and Michelle T. Z. Spence. The molecular probes handbook: A guide to fluorescent probes and labeling technologies. [Carlsbad, CA]: Live Technologies Corporation, 2010.

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Jan, Slavík. Fluorescent probes in cellular and molecular biology. Boca Raton: CRC Press, 1994.

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Z, Spence Michelle T., and Johnson Iain D, eds. Handbook of fluorescent probes and research chemicals. 6th ed. Eugene, OR, USA (4849 Pitchford Ave., Eugene 97402): Molecular Probes, 1996.

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Haugland, Richard P. Handbook of fluorescent probes and research chemicals. Eugene, OR, USA (4849 Pitchford Ave., Eugene 97402): Molecular Probes, 1989.

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Book chapters on the topic "Fluorescents probes"

1

Slavík, Jan. "Fluorescent Probes." In Fluorescence Microscopy and Fluorescent Probes, 57–60. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_5.

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Herman, Brian. "Fluorescence Microscopy: State of the Art." In Fluorescence Microscopy and Fluorescent Probes, 1–14. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_1.

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Tengholm, Anders, and Eva Grapengiesser. "Disappearance of Cytoplasmic Ca2+ Oscillations is a Sensitive Indicator of Photodamage in Pancreatic β-Cells." In Fluorescence Microscopy and Fluorescent Probes, 85–89. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_10.

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Cimprich, Petr, and Jan Slavík. "Distribution of Individual Cytoplasmic pH Values in a Cell Suspension." In Fluorescence Microscopy and Fluorescent Probes, 91–93. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_11.

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Gregor, Martin, Jan Tachezy, and Jan Slavík. "The Effect of Lysosomal pH on Lactoferrin-Dependent Iron Uptake in Tritrichomonas foetus." In Fluorescence Microscopy and Fluorescent Probes, 95–99. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_12.

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Opitz, N., T. Porwol, E. Merten, and H. Acker. "On the Protein-Error of the Calcium-Sensitive Fluorescent Indicator Fura-Red." In Fluorescence Microscopy and Fluorescent Probes, 101–6. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_13.

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Opitz, N., T. Porwol, E. Merten, and H. Acker. "Cytoplasmic Ion Imaging: Evidence for Intracellular Calibration Heterogeneities of Ion-Sensitive Fluoroprobes." In Fluorescence Microscopy and Fluorescent Probes, 107–12. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_14.

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Plášek, Jaromír, Jan Jaap ter Horst, Marcel Ameloot, and Paul Steels. "The Effect of Protein Binding on the Calibration Curve of the pH Indicator BCECF." In Fluorescence Microscopy and Fluorescent Probes, 113–18. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_15.

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Albrechtová, Jolana T. P., Jan Slavík, and Edgar Wagner. "Use of Fluorescent Probes and CLSM for pH-Monitoring in the Whole Plant Tissue. pH-Changes in the Shoot Apex of Chenopodium rubrum Related to Organogenesis." In Fluorescence Microscopy and Fluorescent Probes, 125–32. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_17.

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Anderson, Robert E., Frederic B. Meyer, and T. M. Sundt. "Spatial Resolution of Cortical Cerebral Blood Flow and Brain Intracellular pH as Measured by In Vivo Fluorescence Imaging." In Fluorescence Microscopy and Fluorescent Probes, 133–38. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_18.

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Conference papers on the topic "Fluorescents probes"

1

Sayers, Michael B., and Tara M. Dalton. "A Real-Time Continuous Flow Polymerase Chain Reactor for DNA Expression Quantification." In ASME 2007 International Mechanical Engineering Congress and Exposition. ASMEDC, 2007. http://dx.doi.org/10.1115/imece2007-43058.

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Real-time quantitative Polymerase Chain Reaction (PCR) is an extremely sensitive and reliable method for quantifying gene expression, allowing subtle shifts in gene expression to be easily monitored. Currently, stationary real-time PCR is readily achieved using fluorescent labels which increase in fluorescence as the DNA is exponentially amplified. Quantitative PCR is used in a myriad of applications. However currently most commercial real-time PCR devices are batch process stationary well based systems, limiting their throughput. Continuous flow microfluidic PCR devices have allowed for advancement in terms of improved PCR throughput and reduced reagent usage. As part of an overall total analysis system a device integrating all the functional steps of continuous flow realtime quantitative PCR has been designed and fabricated. Initially the PCR reaction mixture is segmented into nano-litre PCR reactors which are then thermally cycled on a two temperature fifty cycle flow-through PCR device, which allows laser induced fluorescent imaging of the nanoreactors. Previous studies into continuous flow PCR have demonstrated endpoint fluorescent measurements, however this research allows PCR nanoreactors to be fluorescently monitored after every PCR thermal cycle. Fluorescent optical monitoring is achieved through laser excitation of the nanoreactors while a Charged Coupled Device (CCD) camera is used to record the fluorescent emissions from the nanoreactors. Intensity analysis of the recorded images is then preformed using MATLAB to accurately determine the fluorescence intensity level, thereby allowing real-time quantitative amplification curves to be generated. This has major advantages over existing continuous flow PCR devices which use endpoint fluorescence and capillary electrophoresis, as the amplification curves allow far more information to be gleaned and allow the initial DNA template concentration to be accurately determined.
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Maruoka, Shoji, Yohei Mitsui, Shinpei Okawa, Yoko Hoshi, and Yukio Yamada. "Measurement of Fluorescence Properties in Light Scattering Medium." In ASME/JSME 2011 8th Thermal Engineering Joint Conference. ASMEDC, 2011. http://dx.doi.org/10.1115/ajtec2011-44458.

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The purpose of this study is to measure the fluorescence properties of Indocyaninegreen (ICG) which is a fluorescence dye to be used as a fluorescence probe for the use of fluorescence imaging in biomedical applications. The fluorescence molecular imaging is expected to solve the issues in preclinical studies which require a lot of time, labors and sacrificed animals. Information of living body can be obtained by measuring the fluorescent properties of the probe in biological media. The absorption and emission spectra and the lifetime of ICG in non-scattering and scattering media were measured in this study. ICG was dissolved in water, in plasma, in Intralipid, and in a mixture of plasma and Intralipid to simulate the environment in living tissues. The absorption and emission spectra were measured using a fluorescence spectrophotometer. The fluorescence lifetimes were measured using a time-resolved measurement method. Results suggest that the fluorescent properties are affected by the reaction between ICG and biological tissues.
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Fiedor, Pawel, Joanna Ortyl, and Mariusz Galek. "MICROWAVE-ASSISTED SYNTHESIS AND SPECTROSCOPIC PROPERTIES OF NOVEL PYRIDINE-BASED FLUORESCENT MOLECULAR PROBES." In Ampere 2019. Valencia: Universitat Politècnica de València, 2019. http://dx.doi.org/10.4995/ampere2019.2019.9829.

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Fluorescent molecular probes become interesting analytical tools in determination and labeling of chemical compounds and physical properties such as viscosity and polarity. Currently known fluorescent molecular probes can selectively and regardless of the environment detect only few molecules, and applicability in determination of micro- viscosity and micro-polarity are limited to narrow range and specific condition, therefore design and synthesis of novel molecular probes with extended range of operation are highly needed [1]. Traditional synthesis of 2-amino-4,6-diphenyl-pyridine-3-carbonitrile’s requires two step reaction with long heating time or and toxic solvent. By application of microwave irradiation, reaction time can be firmly shortened with the same or higher efficiency [2]. Derivatives of 2-amino-4,6-diphenyl-pyridine-3-carbonitrile can find application in different fields of science. Depending on the structure of fluorophore, those compounds exhibit high sensitivity to changes in polarity and viscosity of environment, also concentration of specific cations, and pH can be determined by measuring of fluorescence spectrum.
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Drobizhev, Mikhail, J. Nathan Scott, Patrik R. Callis, Rosana S. Molina, and Thomas E. Hughes. "Role of Local Electric Field in Controlling Fluorescence Quantum Yield of Red Fluorescent Proteins." In Optical Molecular Probes, Imaging and Drug Delivery. Washington, D.C.: OSA, 2019. http://dx.doi.org/10.1364/omp.2019.ot2d.5.

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Fron, Damien J., Alptekin Aksan, and John J. McGrath. "Ratiometric Laser-Induced Fluorescence (LIF) Thermography of Radiofrequency Heated Tissue Phantoms." In ASME 2002 International Mechanical Engineering Congress and Exposition. ASMEDC, 2002. http://dx.doi.org/10.1115/imece2002-32353.

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A ratiometric laser-induced fluorescence (LIF) thermographic method has been developed to quantify the temperature fields associated with radiofrequency (RF) heating probes used to treat joint instabilities associated with the shoulder, knee, and ankle. Accurate temperature field measurements in tissue phantoms represent invaluable data for the purpose of developing and verifying computer models of tissue heating. Measuring temperature fields associated with RF probe heating in physiological environments poses problems for traditional methods. Thermocouples, infrared thermometry and fluoroptic methods are limited due to inability of thermocouples to operate in an RF environment, the opaque nature of physiological solutions to IR wavelengths and the relatively large size of optical fibers used in fluoroptic thermometry respectively. Whole field temperature mapping and heat flux measurements around monopolar and bipolar radiofrequency (RF) probes have been performed in tissue phantoms using two-color laser induced fluorescence (LIF) and the ratio of the fluorescent emission intensities of Rhodamine B and Rhodamine 110. The method is described, the operating characteristics are defined and examples of the application of the method to radiofrequency (RF) heating in phantom tissues are included.
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Kushnir, A. V., Pjotr M. Larionov, E. E. Litasova, Alexey N. Malov, M. M. Mandrik, Nikolai A. Maslov, Anatoliy M. Orishich, and M. B. Vasilieva. "Study of heart tissue viability using fluorescent probes and native laser-induced fluorescence." In Seventh International Symposium on Laser Metrology Applied to Science, Industry, and Everyday Life, edited by Yuri V. Chugui, Sergei N. Bagayev, Albert Weckenmann, and P. Herbert Osanna. SPIE, 2002. http://dx.doi.org/10.1117/12.484500.

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Lin, T. I., M. V. Jovanovic, and R. M. Dowben. "Nine New Fluorescent Probes." In OE/LASE '89, edited by Gary C. Salzman. SPIE, 1989. http://dx.doi.org/10.1117/12.951899.

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Casay, Guillermo A., Tibor Czuppon, Jacek Lipowski, and Gabor Patonay. "Near-infrared fluorescent probes." In OE/LASE'93: Optics, Electro-Optics, & Laser Applications in Science& Engineering, edited by Joseph R. Lakowicz and Richard B. Thompson. SPIE, 1993. http://dx.doi.org/10.1117/12.144722.

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Belfield, Kevin D., Sheng Yao, Bosung Kim, and Xiling Yue. "Fluorenyl benzothiadiazole and benzoselenadiazole near-IR fluorescent probes for two-photon fluorescence imaging (Conference Presentation)." In Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VIII, edited by Samuel Achilefu and Ramesh Raghavachari. SPIE, 2016. http://dx.doi.org/10.1117/12.2211199.

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Kilin, Vasyl, Zeinab Darwich, Ludovic Richert, Pascal Didier, Andrey Klymchenko, and Yves Mély. "Two photon fluorescence imaging of lipid membrane domains and potentials using advanced fluorescent probes." In SPIE BiOS, edited by Ammasi Periasamy, Karsten König, and Peter T. C. So. SPIE, 2013. http://dx.doi.org/10.1117/12.2001492.

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Reports on the topic "Fluorescents probes"

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Marrone, B. L., L. L. Deaven, D. J. Chen, Min S. Park, M. A. MacInnes, G. C. Salzman, and T. M. Yoshida. Directly labeled fluorescent DNA probes for chromosome mapping. Office of Scientific and Technical Information (OSTI), December 1995. http://dx.doi.org/10.2172/205135.

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Chen, Yuxiang. Pathogen Light: Fluorescent Probe for Rapid Foodborne Bacteria Detection. Office of Scientific and Technical Information (OSTI), April 2019. http://dx.doi.org/10.2172/1507303.

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Kool, Eric T. Sensitive, Selective Fluorescent Probes for the Detection and Identification of Nucleic Acids. Fort Belvoir, VA: Defense Technical Information Center, March 2001. http://dx.doi.org/10.21236/ada394269.

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Suttle, Curtis. Development and Use of Fluorescent Probes for Detection, Enumeration and Identification of Naturally-Occurring Marine Viruses. Fort Belvoir, VA: Defense Technical Information Center, May 1992. http://dx.doi.org/10.21236/ada256531.

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Isailovic, Dragan. Subunits of highly Fluorescent Protein R-Phycoerythrin as Probes for Cell Imaging and Single-Molecule Detection. Office of Scientific and Technical Information (OSTI), January 2005. http://dx.doi.org/10.2172/861609.

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Mazel, Charles. A Multispectral Fluorescence and Reflectance Probe for In Situ Characterization of Benthic Environments. Fort Belvoir, VA: Defense Technical Information Center, September 1999. http://dx.doi.org/10.21236/ada630464.

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Paczkowski, Jerzy, Bozena Paczkowska, and D. C. Neckers. Studies of the Kinetics of Polyolmethacrylate Polymerization by Fluorescence Probes. Part 3. Polymeric Photinitiators and Coinitiators. Fort Belvoir, VA: Defense Technical Information Center, June 1995. http://dx.doi.org/10.21236/ada296546.

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Dittmer, Joy. Directly incorporating fluorochromes into DNA probes by PCR increases the efficience of fluorescence in situ hybridization. Office of Scientific and Technical Information (OSTI), May 1996. http://dx.doi.org/10.2172/576746.

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Rathbone, R. F., J. C. Hower, and F. J. Derbyshire. Coal liquefaction process streams characterization and evaluation. Novel analytical techniques for coal liquefaction: Fluorescence microscopy. Office of Scientific and Technical Information (OSTI), October 1991. http://dx.doi.org/10.2172/10123118.

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Syed, Aleem. Spatial and temporal dynamics of receptor for advanced glycation endproducts, integrins, and actin cytoskeleton as probed with fluorescence-based imaging techniques. Office of Scientific and Technical Information (OSTI), January 2016. http://dx.doi.org/10.2172/1342583.

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