Relevant bibliographies by topics / Fluorometric techniques

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Academic literature on the topic 'Fluorometric techniques'

Author: Grafiati
Published: 4 June 2021
Last updated: 10 February 2022

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Journal articles on the topic "Fluorometric techniques"

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Spierto, F. W., T. L. Hearn, F. H. Gardner, and W. H. Hannon. "Phenylalanine analyses of blood-spot control materials: preparation of samples and evaluation of interlaboratory performance." Clinical Chemistry 31, no. 2 (February 1, 1985): 235–38. http://dx.doi.org/10.1093/clinchem/31.2.235.

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Abstract Aliquots (0.1 mL) of whole-blood pools prepared to contain various concentrations of phenylalanine were applied to filter-paper collection cards, dried, and stored in sealed bags. We measured the phenylalanine content of the dried blood spots by bioassay, fluorometry, and "high-performance" liquid chromatography, and found that the concentrations remained constant for two years when samples were kept at -20 degrees C or lower. Intra- and interlaboratory studies showed that results for phenylalanine were greater for laboratories using bioassay procedures than for those using fluorometric procedures. Further, CVs (both among- and within-laboratory) obtained with fluorometric procedures were nearly half as great as the CVs obtained by laboratories using bioassay techniques.
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Wolszczak, Marian, and Jacek Miller. "Characterization of non-ionic surfactant aggregates by fluorometric techniques." Journal of Photochemistry and Photobiology A: Chemistry 147, no. 1 (February 2002): 45–54. http://dx.doi.org/10.1016/s1010-6030(01)00611-6.

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Cook, Sarah, Hui-Ling Chan, Soroush Abolfathi, Gary D. Bending, Hendrik Schäfer, and Jonathan M. Pearson. "Longitudinal dispersion of microplastics in aquatic flows using fluorometric techniques." Water Research 170 (March 2020): 115337. http://dx.doi.org/10.1016/j.watres.2019.115337.

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Wink, David A., Sungmee Kim, Allen Miles, David Jourd'heuil, and Matthew B. Grisham. "Fluorometric Techniques for the Detection of Nitric Oxide and Metabolites." Current Protocols in Toxicology 00, no. 1 (May 1999): 10.4.1–10.4.12. http://dx.doi.org/10.1002/0471140856.tx1004s00.

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Gelb, Michael H., C. Ronald Scott, and Frantisek Turecek. "Newborn Screening for Lysosomal Storage Diseases." Clinical Chemistry 61, no. 2 (February 1, 2015): 335–46. http://dx.doi.org/10.1373/clinchem.2014.225771.

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Abstract BACKGROUND There is worldwide interest in newborn screening for lysosomal storage diseases because of the development of treatment options that give better results when carried out early in life. Screens with high differentiation between affected and nonaffected individuals are critical because of the large number of potential false positives. CONTENT This review summarizes 3 screening methods: (a) direct assay of enzymatic activities using tandem mass spectrometry or fluorometry, (b) immunocapture-based measurement of lysosomal enzyme abundance, and (c) measurement of biomarkers. Assay performance is compared on the basis of small-scale studies as well as on large-scale pilot studies of mass spectrometric and fluorometric screens. SUMMARY Tandem mass spectrometry and fluorometry techniques for direct assay of lysosomal enzymatic activity in dried blood spots have emerged as the most studied approaches. Comparative mass spectrometry vs fluorometry studies show that the former better differentiates between nonaffected vs affected individuals. This in turn leads to a manageable number of screen positives that can be further evaluated with second-tier methods.
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Cicerelli, Rejane Ennes, Maria de Lourdes B. Trindade Galo, and Henrique Llacer Roig. "Multisource data for seasonal variability analysis of cyanobacteria in a tropical inland aquatic environment." Marine and Freshwater Research 68, no. 12 (2017): 2344. http://dx.doi.org/10.1071/mf16259.

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Cyanobacterial blooms are related to eutrophic conditions that compromise the many uses of reservoirs. Thus, quick and effective methods for detecting the abundance of cyanobacteria in waterbodies are needed to complement conventional laboratory methods. In addition, inadequate control techniques that are applied at times of high cyanobacterial concentrations can cause the cells to lyse and release toxins into the water. In the present study we investigated the behaviour of cyanobacteria by determining phycocyanin and chlorophyll concentrations, using spectroradiometric and fluorometric techniques, in three field campaigns performed at the Nova Avanhandava Reservoir, Brazil. The sampling rate and favourable season for data collected had been determined previously by remote sensing analysis. Seasonal estimates of cyanobacteria were made because fluorometric sensors were able to record low concentrations, whereas the spectral analyses only detected phycocyanin at higher concentrations. Results of spectral analyses highlighted the subtle spectral characteristics indicating the presence of phycocyanin, even without a clear definition of the diagnostic features in the reflectance curve. Therefore, multiscale remote sensing complemented by fluorometric analysis and relevant environmental variables is an effective approach for monitoring cyanobacteria in Brazilian inland waters.
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Ghosh, Saptarshi, and Nitin Chattopadhyay. "Modification of the photophysics of 3-hydroxyflavone in aqueous solutions of imidazolium-based room temperature ionic liquids: a comparison between micelle-forming and non micelle-forming ionic liquids." RSC Advances 5, no. 61 (2015): 49054–61. http://dx.doi.org/10.1039/c5ra03833b.

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Fluorometric techniques have been exploited to study the photophysical behaviour of an ESIPT probe, 3HF, in two imidazolium-based room temperature ionic liquids, one micelle-forming and the other non micelle-forming.
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Gooijer, Cees. "Fluorometric Analysis in Biomedical Chemistry—Trends and Techniques including HPLC Applications." Analytica Chimica Acta 264, no. 2 (July 1992): 369. http://dx.doi.org/10.1016/0003-2670(92)87027-i.

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Baeyens, WillyR G. "Fluorometric analysis in biomedical chemistry -Trends and techniques including HPLC applications." Journal of Chromatography A 590, no. 2 (January 1992): 373–74. http://dx.doi.org/10.1016/0021-9673(92)85402-f.

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Petersen, OH. "Control of Insulin Secretion in Pancreatic Beta-Cells." Physiology 5, no. 6 (December 1, 1990): 254–58. http://dx.doi.org/10.1152/physiologyonline.1990.5.6.254.

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Recent studies on single cells employing both fluorometric and electrophysiological techniques reveal new aspects of metabolic regulation of cellular Ca2+ handling. Glucose-evoked increase in metabolism precedes Ca2+ influx through voltage-gated Ca2+ channels, and metabolism controls Ca2+ channel opening, not only via membrane potential changes but also more directly.
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Dissertations / Theses on the topic "Fluorometric techniques"

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Hojati, Ashkhan. "Pharmacologic profiling of novel compounds via fluorometric analyses of monoamine transporter responses." VCU Scholars Compass, 2019. https://scholarscompass.vcu.edu/etd/5983.

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In humans and other organisms, monoaminergic systems are crucial in neuronal function and behavior. The monoamine transporters (MATs), which can be found on the presynaptic plasma membrane of neurons in the central nervous system (CNS), are crucial in the regulation of neurotransmitter concentration in the synaptic cleft. As the duration and concentration of neurotransmitters in the cleft affect further downstream signaling responses, these proteins are important targets for both understanding neuronal physiology and compounds of interest. Multiple theories exist proponing the contribution of MATs to a variety of mental and neurological disorders, including depression. This theory establishes that depression is caused by imbalances in monoamine neurotransmitters. Compounds such as Fluoxetine (FLX) are classified as selective serotonin reuptake inhibitors (SSRIs), these drugs selectively block the reuptake of neurotransmitters at the serotonin transporter (SERT). Since differences in MAT selectivity of inhibitory compounds are influential to selecting efficacious antidepressant treatments, we utilized a unique fluorescent analysis technique to explore three therapeutic compounds of interest (in-vitro) which contain structural similarity to FLX. Our results confirm the selectivity of FLX at SERT, and classify the novel compounds studied into different potential categories of reuptake inhibitors. We hope these compounds will be studied further to elucidate their potentially therapeutic roles and mitigation of undesired side effects seen in other medications.
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Seyitmuhammedov, Kyyas. "An Assessment of Fluorometric Techniques for Tracking the Transport of Polycyclic Aromatic Hydrocarbons from Groundwater into Surface Water Bodies." Digital WPI, 2014. https://digitalcommons.wpi.edu/etd-theses/915.

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"A number of fluorometric techniques have been applied to characterize contamination associated with oil discharges and spills in the environment. While these techniques provide quick and lower cost alternatives to the many of the advanced techniques for characterizing oil-related constituents, their applicability still isn’t fully understood. The objectives of this research were to understand the characteristics of organic transport in a linked surface-water/ground-water system, and develop some practical approaches using fluorometry to characterize the pathways of organic transport. The approach included modeling, field sampling and comparisons of laboratory analyses to assess basic field fluorometry techniques for characterizing sources and distributions of polycyclic aromatic hydrocarbons (PAHs) associated with oil discharges. The primary field site included a canal and nearby river, which resulted in generally uniform hydraulic gradient, such that petroleum and PAH contamination at the site could be characterized. Historical data provided general information on the distribution of contamination. Modeling using the Modflow groundwater flow package provided basic information on groundwater flow pathways and rates. Samples were collected from the canal, groundwater, the river and a treatment facility. Additional samples were collected from Bayou Corne sinkhole in Lousiana and the Deepwater Horizon crude oil spill in the Gulf of Mexico. The samples were analyzed for fluorometric absorbance using a 10AU field fluorometer, a Shimadzu absorbance spectrometer and a LS5 luminescence spectrometer (which provided fluorescence over a spectrum of frequencies). Additional analyses were completed using a gas chromatograph with a flame ionization detector (GC-FID) to provide a more complete qualitative description of the oil composition. Analysis of the results from the 10-AU field fluorometer confirmed the capability of the field fluorometer to detect organic contamination resulting from crude and refined oil spills. Absorbance spectrometer results demonstrated possibility of using the PAH absorbance spectra to distinguish between the different types of oil, although more detailed analyses using various types of oil is recommended. The results using the luminescence spectrometer were consistent with GC FID results, and provided useful comparisons indicating the characteristics of fresh and weathered oil. The comparisons provide insight into the applicability of fluorometric approaches for characterizing transport pathways and concentrations of organic constituents associated with discharges of oil and other PAHs."
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Prayoonpokarach, Sanchai. "Development and evaluation of sampling techniques, instrumentation, and pyridine derivative reagents for fluorometric determination of chloroform and TCE in water with a portable fluorometer." Thesis, 2003. http://hdl.handle.net/1957/30845.

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A novel, portable, filter fluorometer was developed for the determination of chloroform and TCE at environmentally-relevant levels when coupled with improved sampling techniques and reagents. Reagents selective for the TCE or chloroform convert these toxic species into fluorescent species that can be monitored. The fluorometer is based on LED excitation light sources, a battery-operated photomultiplier tube as a radiation detector, and appropriate excitation and emission filters. A unique low-power, miniature heater inside the cell holder of the fluorometer provides control of the temperature of the reagent solution above ambient temperature. The fluorometer and the sampling systems, including a miniature air pump, are portable and can be operated from a small lead battery over an entire day. Sparging, passive transfer, and membrane sampling techniques were used to transfer TCE or chloroform from the sample solution as a vapor into the appropriate reagent and to provide preconcentration. The apparatus for membrane sampling was improved to be applicable for continuous sampling of water in the field situations with minimal sample manipulation. Each of the three sampling techniques provides a transfer rate of the analyte of ~1 ng/min per ng/mL of analyte in the sample. The optimized reagent based on 1-(3-pyridylmethyl)urea provides high selectivity to chloroform and the reagent based on isonicotinamide has excellent selectivity to TCE. These two reagents serve as an alternative to the more common pyridine reagent for the determination of chloroform or TCE in water and eliminate the exposure of the user to toxic pyridine vapor. The developed filter fluorometer, the optimized reagents, and the membrane sampling technique provide a detection limit for chloroform of 0.2 and 10 ng/mL, respectively, with the pyridine and 1-(3-pyridylmethyl)urea reagent. The detection limit for TCE is 0.3 ng/mL with the isonicotinamide reagent. For TCE, the detection limit is almost two orders of magnitude better than obtained previously with a fluorometric technique. Analysis times vary from 15 to 30 min.
Graduation date: 2003
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Books on the topic "Fluorometric techniques"

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Norio, Ichinose, ed. Fluorometric analysis in biomedical chemistry: Trends and techniques including HPLC applications. New York: Wiley, 1991.

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Ichinose, Norio, George Schwedt, Frank Michael Schnepel, and Kyoko Adachi. Fluorometric Analysis in Biomedical Chemistry: Trends and Techniques Including HPLC Applications (Chemical Analysis: A Series of Monographs on Analytical Chemistry and Its Applications). Wiley-Interscience, 1991.

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Bultynck, Geert, Jan B. Parys, Martin Bootman, and David I. Yule. Calcium Techniques. Cold Spring Harbor Laboratory Press, 2013.

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F, Wilkinson M. H., and Schut F, eds. Digital image analysis of microbes: Imaging, morphometry, fluorometry, and motility techniques and applications. Chichester: Wiley, 1998.

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M. H. F. Wilkinson (Editor) and F. Schut (Editor), eds. Digital Image Analysis of Microbes: Imaging, Morphometry, Fluorometry and Motility Techniques and Applications. Wiley, 1998.

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Schut, F., and M. H. F. Wilkinson. Digital Image Analysis of Microbes: Imaging, Morphometry, Fluorometry and Motility Techniques and Applications. Wiley & Sons, Incorporated, John, 2008.

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Bultynck, Geert, Jan B. Parys, Martin Bootman, and David I. Yule. Calcium Techniques: A Laboratory Manual. Cold Spring Harbor Laboratory Press, 2013.

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Book chapters on the topic "Fluorometric techniques"

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Masters, Barry R. "In Vivo Corneal Redox Fluorometry." In Noninvasive Diagnostic Techniques in Ophthalmology, 223–47. New York, NY: Springer New York, 1990. http://dx.doi.org/10.1007/978-1-4613-8896-8_14.

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Brubaker, Richard F., David M. Maurice, and Jay W. McLaren. "Fluorometry of the Anterior Segment." In Noninvasive Diagnostic Techniques in Ophthalmology, 248–80. New York, NY: Springer New York, 1990. http://dx.doi.org/10.1007/978-1-4613-8896-8_15.

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Gandhi, Chris S., and Riccardo Olcese. "The Voltage-Clamp Fluorometry Technique." In Methods in Molecular Biology, 213–31. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-526-8_17.

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Suárez-Delgado, Esteban, and León D. Islas. "Patch-Clamp Fluorometry and Its Applications to the Study of Ion Channels." In Basic Neurobiology Techniques, 155–83. New York, NY: Springer US, 2019. http://dx.doi.org/10.1007/978-1-4939-9944-6_7.

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Greenberg, J. H., D. Uematsu, and M. Reivich. "A Fluorometric Technique for the In Vivo Measurement of Intracellular Calcium." In Cerebral Ischemia and Calcium, 155–61. Berlin, Heidelberg: Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-85863-5_20.

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Hausmann, M., C. Cremer, P. Popescu, and D. Celeda. "Application of Flow Cytometry and Slit-Scan Fluorometry in Mammalian Chromosomes Analysis and Sorting." In Techniques in Animal Cytogenetics, 157–86. Berlin, Heidelberg: Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-59711-4_8.

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Phillips, R. E., J. Stefanavage, M. F. Moulthrop, and C. M. Johnson. "Fluorometry and Nephelometry: Techniques and Uses in Soil, Plant, and Water Analysis." In Instrumental Methods for Analysis of Soils and Plant Tissue, 113–27. Madison, WI, USA: Soil Science Society of America, 2015. http://dx.doi.org/10.2136/1971.instrumentalmethods.c7.

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"High-Performance Liquid Chromatographic Mapping of Oligosaccharides Using High pH Anion-Exchange Chromatography: Improvements from Sample Preparation, Reduction, and Fluorometric Detection." In Techniques in Glycobiology, 467–80. CRC Press, 1997. http://dx.doi.org/10.1201/b15312-34.

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Lakowicz, J. R., J. Kuśba, I. Gryczynski, H. Szmacinski, W. Wiczk, and M. L. Johnson. "Macromolecular Dynamics Observed by Intramolecular Energy Transfer Using Frequency-Domain Fluorometry." In Techniques in Protein Chemistry III, 429–36. Elsevier, 1992. http://dx.doi.org/10.1016/b978-0-12-058756-8.50048-1.

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Conference papers on the topic "Fluorometric techniques"

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Kandlakunta, Sahithi, and Mahesh Panchagnula. "Laser Induced Fluorometry and Velocimetry." In ASME 2006 International Mechanical Engineering Congress and Exposition. ASMEDC, 2006. http://dx.doi.org/10.1115/imece2006-14980.

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The characteristics of fuel atomizers have been studied by using a fluorescence optical patternator via establishing a laser sheet illumination of the spray and an image capturing system. Line laser Mie-scattering and fluorescence imaging technique is used to study the fuel mass distribution, geometrical properties, angle and symmetry in sprays. The proposed experimental setup employs Rhodamine 6G as the fluorophore. A set of filters have been used to reduce the signature from the combustion fire while being able to image the nanoparticles. Experimental results are obtained under the conditions of the fuel with and without being seeded with quantum dots and under both non-combusting and combusting spray conditions. The results from the study are validated against existing volume flux distribution measurements by conventional techniques. Owing to the high luminescence properties of quantum dots, the liquid volume distribution can accurately be determined in an evaporating as well as a non-evaporating spray using this technique. Quantum dots are semiconductor nanoparticles whose emission wavelength can be tuned by the choice of their size. Also their, high luminescence properties are advantageous in a spectrally "noisy" combustion environment.
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Shanshan Zhang, Rongguo Su, Yali Duan, Lihong Yan, Cui Zhang, and Xiulin Wang. "A fluorometric differentiation technique of phytoplankton assemblage based on Coif2 wavelet and fourth-derivative." In 2011 International Conference on Remote Sensing, Environment and Transportation Engineering (RSETE). IEEE, 2011. http://dx.doi.org/10.1109/rsete.2011.5966055.

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Bottiroli, Giovanni F., Roberta Ramponi, Anna C. Croce, Giuseppe Rescigno, Stefano Nazari, and Aris Zonta. "High-frequency perfusion fluorometry: a new technique to assess bronchial mucosa blood perfusion." In OE/LASE '92, edited by Thomas S. Mang. SPIE, 1992. http://dx.doi.org/10.1117/12.59366.

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Al-Naema, Nayla, Suhur Saeed, Nandita Deb, and Jennifer Dupont. "Pulse Amplitude Modulation Fluorometry Technique & Laboratory Cultivation of Qatari Corals: Studying Coral Growth and Photosynthetic Efficiency." In SPE Middle East Health, Safety, Environment & Sustainable Development Conference and Exhibition. Society of Petroleum Engineers, 2014. http://dx.doi.org/10.2118/170369-ms.

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Jofre, M., J. M. Perez, P. Martinez, Z. Moubarak, C. Hurth, MA Yanez, V. Catalan, A. Parker, M. Veldhuis, and V. Prunei. "CMOS-based Image Cytometry for Detection of Phytoplankton in Ballast Water." In IMarEST Ballast Water Technology Conference. IMarEST, 2017. http://dx.doi.org/10.24868/bwtc6.2017.005.

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An image cytometer (CYT) for the analysis of phytoplankton in fresh and marine water environments is introduced. A linear quantification of the number of cells over several orders of magnitude of concentrations was observed using cultures of Tetraselmis and Nannochloropsis measured by autofluorescence of the chlorophyll in a laboratory environment. The functionality of the system outside the laboratory was analysed by phytoplankton quantification of samples taken from marine water environment (Dutch Wadden Sea, The Netherlands) and fresh water environment (Lake Ijssel, The Netherlands). The CYT was also employed to study the effects of two ballast water treatment systems (BWTS), based on chlorine electrolysis and UV sterilisation by determining the vitality of the phytoplankton. In order to ensure the detection limit, a large volume (1l) of samples was collected and concentrated to 3 ml using CelltrapTM filters. The results were compared to benchmarked flow cytometer and PAM Fluorometry at Marine Eco-Analytics (MEA-NL). The image cytometer reached a 10 cells/ml limit of detection (LoD) with an accuracy between 0.7 and 0.5 log, and a correlation of 88.29% in quantification and 96.21% in vitality, when compared to benchmarked monitoring techniques.
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Reports on the topic "Fluorometric techniques"

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Yinfa, Ma. Indirect fluorometric detection techniques on thin layer chromatography and effect of ultrasound on gel electrophoresis. Office of Scientific and Technical Information (OSTI), December 1990. http://dx.doi.org/10.2172/6045672.

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