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Journal articles on the topic 'FM dye'

Author: Grafiati

Published: 4 June 2021

Last updated: 4 February 2022

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1

Kane, D. M., S. R. Bramwell, and A. I. Ferguson. "FM dye lasers." Applied Physics B Photophysics and Laser Chemistry 39, no. 3 (1986): 171–78. http://dx.doi.org/10.1007/bf00697415.

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2

Hoopmann, P., S. O. Rizzoli, and W. J. Betz. "FM Dye Photoconversion for Visualizing Synaptic Vesicles by Electron Microscopy." Cold Spring Harbor Protocols 2012, no. 1 (2011): pdb.prot067611. http://dx.doi.org/10.1101/pdb.prot067611.

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3

Kane, D. M., S. R. Bramwell, and A. I. Ferguson. "Comparison of FM dye lasers in standing-wave and ring configuration." Applied Physics B Photophysics and Laser Chemistry 40, no. 3 (1986): 147–51. http://dx.doi.org/10.1007/bf00697244.

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4

Vida, T. A., and S. D. Emr. "A new vital stain for visualizing vacuolar membrane dynamics and endocytosis in yeast." Journal of Cell Biology 128, no. 5 (1995): 779–92. http://dx.doi.org/10.1083/jcb.128.5.779.

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We have used a lipophilic styryl dye, N-(3-triethylammoniumpropyl)-4- (p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM 4-64), as a vital stain to follow bulk membrane-internalization and transport to the vacuole in yeast. After treatment for 60 min at 30 degrees C, FM 4-64 stained the vacuole membrane (ring staining pattern). FM 4-64 did not appear to reach the vacuole by passive diffusion because at 0 degree C it exclusively stained the plasma membrane (PM). The PM staining decreased after warming cells to 25 degrees C and small punctate structures became apparent in the cytoplasm

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5

Hansen, Gert H., Karina Rasmussen, Lise-Lotte Niels-Christiansen, and E. Michael Danielsen. "Endocytic trafficking from the small intestinal brush border probed with FM dye." American Journal of Physiology-Gastrointestinal and Liver Physiology 297, no. 4 (2009): G708—G715. http://dx.doi.org/10.1152/ajpgi.00192.2009.

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The small intestinal brush border functions as the body's main portal for uptake of dietary nutrients and simultaneously acts as the largest permeability barrier against pathogens. To enable this, the digestive enzymes of the brush border are organized in lipid raft microdomains stabilized by cross-linking galectins and intelectin, but little is known about the dynamic properties of this highly specialized membrane. Here, we probed the endocytic membrane trafficking from the brush border of organ-cultured pig intestinal mucosal explants by use of a fixable, lipophilic FM dye. The fluorescent d

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Abdrakhmanov, M. M., A. M. Petrov, P. N. Grigoryev, and A. L. Zefirov. "Depolarization-Induced Calcium-Independent Synaptic Vesicle Exo- and Endocytosis at Frog Motor Nerve Terminals." Acta Naturae 5, no. 4 (2013): 77–82. http://dx.doi.org/10.32607/20758251-2013-5-4-77-82.

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The transmitter release and synaptic vesicle exo- and endocytosis induced by constant current depolarization of nerve terminals were studied by microelectode extracellular recording of miniature endplate currents and fluorescent microscopy (FM 1-43 styryl dye). Depolarization of the plasma membrane of nerve terminals in the control specimen was shown to significantly increase the MEPC frequency (quantal transmitter release) and exocytotic rate (FM 1-43 unloading from the synaptic vesicles preliminarily stained with the dye), which was caused by a rise in the intracellular Ca 2+ concentration d

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7

Niles, Walter. "Changes in Fluorescence Spectra of the Dye FM 1-43 in Different Environments." Microscopy Today 7, no. 7 (1999): 45. http://dx.doi.org/10.1017/s1551929500064944.

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8

Van Oosterhout, M. F., H. M. Willigers, R. S. Reneman, and F. W. Prinzen. "Fluorescent microspheres to measure organ perfusion: validation of a simplified sample processing technique." American Journal of Physiology-Heart and Circulatory Physiology 269, no. 2 (1995): H725—H733. http://dx.doi.org/10.1152/ajpheart.1995.269.2.h725.

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A disadvantage of nonradioactive microsphere techniques is that the processing of samples is time-consuming and complex. We developed and validated a simplified processing method for the fluorescent microsphere (FM) technique. In seven anesthetized dogs with coronary artery stenosis up to six different FM and five different radioactivity labeled microspheres (RM) were injected. Two FM and two RM labels were injected simultaneously to enable inter- and intramethod comparison. After gamma-counting samples of blood, myocardium (n = 168), and other organs (n = 59) were digested in test tubes with

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9

Westling, L. A., and M. G. Raymer. "Intensity autocorrelation measurements and spontaneous FM phase locking in a multimode pulsed dye laser." Journal of the Optical Society of America B 3, no. 6 (1986): 911. http://dx.doi.org/10.1364/josab.3.000911.

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10

Kamin, Dirk, Natalia H. Revelo, and Silvio O. Rizzoli. "FM Dye Photo-Oxidation as a Tool for Monitoring Membrane Recycling in Inner Hair Cells." PLoS ONE 9, no. 2 (2014): e88353. http://dx.doi.org/10.1371/journal.pone.0088353.

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11

Kim, Hyun Jong, JooHan Woo, Yu Ran Nam, Joo Hyun Nam, and Woo Kyung Kim. "Flos Magnoliae and its Constituent Linoleic Acid Suppress T Lymphocyte Activation via Store-Operated Calcium Entry." American Journal of Chinese Medicine 47, no. 07 (2019): 1627–41. http://dx.doi.org/10.1142/s0192415x19500836.

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Intracellular calcium signaling is crucial for type 2 helper T cell and mast cell activation, which is essential for allergic inflammation. It is initiated by antigen-mediated receptor stimulation that triggers store-operated calcium entry (SOCE) via ORAI1 calcium channel. Flos Magnoliae (FM) is widely used to treat allergic diseases such as allergic rhinitis and asthma. Although many studies have reported that FM regulates intracellular calcium signaling, research on the exact type of calcium channel modulated by FM is scarce. Therefore, we hypothesized that the anti-allergic effects of FM mi

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12

Namin, Shabnam M., Sara Nofallah, Mahesh S. Joshi, Konstantinos Kavallieratos, and Nikolaos M. Tsoukias. "Kinetic analysis of DAF-FM activation by NO: Toward calibration of a NO-sensitive fluorescent dye." Nitric Oxide 28 (January 2013): 39–46. http://dx.doi.org/10.1016/j.niox.2012.10.001.

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13

RIBEIRO, F., P. M. Z. COELHO, L. Q. VIEIRA, K. POWELL, and J. R. KUSEL. "Membrane internalization processes in different stages of Schistosoma mansoni as shown by a styryl dye (Frei Mao 1–43)." Parasitology 116, no. 1 (1998): 51–59. http://dx.doi.org/10.1017/s0031182097001960.

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We have used a styryl dye FM 1–43 that cannot cross membranes passively to evaluate membrane vesicle internalization processes from the heptalaminate surface membrane in schistosomula and adult Schistosoma mansoni. The process of dye internalization was faster in adult worms than in 3 h skin or 24-h-old mechanically transformed schistosomula. Dye internalization and vesicle formation were inhibited at low temperature which suggests that the process is energy dependent. Dye internalization was observed to be a result of vesicle formation. This process is impaired by primaquine independently of

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14

Gaffield, Michael A., Christin F. Romberg, and William J. Betz. "Live imaging of bulk endocytosis in frog motor nerve terminals using FM dyes." Journal of Neurophysiology 106, no. 2 (2011): 599–607. http://dx.doi.org/10.1152/jn.00123.2011.

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We observed endocytosis in real time in stimulated frog motor nerve terminals by imaging the growth of large membrane infoldings labeled with a low concentration of FM dye. The spatial and temporal information made available by these experiments allowed us to image several new aspects of this synaptic vesicle recycling pathway. Membrane infoldings appeared near synaptic vesicle clusters and grew rapidly during long-duration, high-frequency stimulation. In some cases, we observed large, elongated infoldings growing laterally into the terminal. We used these observations to calculate infolding g

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15

Mair, Norbert, Thomas Haller, and Paul Dietl. "Exocytosis in alveolar type II cells revealed by cell capacitance and fluorescence measurements." American Journal of Physiology-Lung Cellular and Molecular Physiology 276, no. 2 (1999): L376—L382. http://dx.doi.org/10.1152/ajplung.1999.276.2.l376.

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Measurement of lamellar body (LB) exocytosis at high spatial and temporal resolution was recently enabled by fluorescence of the dye FM 1-43 (FFM1-43). Here, the capabilities of this method were further examined and extended by simultaneous measurement of the cell membrane capacitance ( C m) and laser-scanning confocal microscopy. Step increases in C m were evoked by extracellular ATP (20 μM) or an elevated pipette Ca2+ concentration (≥3 μM). The delay between the first C m step and the increase in FFM1-43 was <1 s, indicating ready access of FM 1-43 to exocytosed LB contents. A specific C

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16

Marra, Vincenzo, Jemima J. Burden, Freya Crawford, and Kevin Staras. "Ultrastructural readout of functional synaptic vesicle pools in hippocampal slices based on FM dye labeling and photoconversion." Nature Protocols 9, no. 6 (2014): 1337–47. http://dx.doi.org/10.1038/nprot.2014.088.

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17

Sweetser, J., T. J. Dunn, I. A. Walmsley, C. Radzewicz, S. Palese, and R. J. D. Miller. "Characterization of an FM mode-locked Nd: YLF laser synchronized with a passively mode-locked dye laser." Optics Communications 97, no. 5-6 (1993): 379–87. http://dx.doi.org/10.1016/0030-4018(93)90507-2.

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18

Buchwalder, Lynn F., Michelle Lin, Thomas J. McDonald, and Peter W. Nathanielsz. "Fetal sheep adrenal blood flow responses to hypoxemia after splanchnicotomy using fluorescent microspheres." Journal of Applied Physiology 84, no. 1 (1998): 82–89. http://dx.doi.org/10.1152/jappl.1998.84.1.82.

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Buchwalder, Lynn F., Michelle Lin, Thomas J. McDonald, and Peter W. Nathanielsz. Fetal sheep adrenal blood flow responses to hypoxemia after splanchnicotomy using fluorescent microspheres. J. Appl. Physiol. 84(1): 82–89, 1998.—Adrenal gland blood flow (ABF) increases during hypoxemia in fetal sheep, but regulation of ABF is poorly understood. The purpose of this study was to determine the effects of splanchnic nerve section on fetal ABF responses to hypoxemia using the fluorescent microsphere (FM) technique. At 125 days of gestation, 14 unanesthetized fetal sheep [bilateral splanchnicotomy (Sp

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19

Nimmervoll, B., B. E. Flucher, and G. J. Obermair. "Dominance of P/Q-type calcium channels in depolarization-induced presynaptic fm dye release in cultured hippocampal neurons." Neuroscience 253 (December 2013): 330–40. http://dx.doi.org/10.1016/j.neuroscience.2013.08.052.

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20

Minshall, Richard D., Chinnaswamy Tiruppathi, Stephen M. Vogel, et al. "Endothelial Cell-Surface Gp60 Activates Vesicle Formation and Trafficking via Gi-Coupled Src Kinase Signaling Pathway." Journal of Cell Biology 150, no. 5 (2000): 1057–70. http://dx.doi.org/10.1083/jcb.150.5.1057.

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We tested the hypothesis that the albumin-docking protein gp60, which is localized in caveolae, couples to the heterotrimeric GTP binding protein Gi, and thereby activates plasmalemmal vesicle formation and the directed migration of vesicles in endothelial cells (ECs). We used the water-soluble styryl pyridinium dye N-(3-triethylaminopropyl)-4-(p-dibutylaminostyryl) pyridinium dibromide (FM 1-43) to quantify vesicle trafficking by confocal and digital fluorescence microscopy. FM 1-43 and fluorescently labeled anti-gp60 antibody (Ab) were colocalized in endocytic vesicles within 5 min of gp60 a

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21

Zheng, Liang-Hong, Chang-He Wang, Shu-Jiang Shang, et al. "Real-time endocytosis imaging as a rapid assay of ligand-GPCR binding in single cells." American Journal of Physiology-Cell Physiology 305, no. 7 (2013): C751—C760. http://dx.doi.org/10.1152/ajpcell.00335.2012.

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Most G protein-coupled receptors (GPCRs) do not generate membrane currents in response to ligand-receptor binding (LRB). Here, we describe a novel technique using endocytosis as a bioassay that can detect activation of a GPCR in a way analogous to patch-clamp recording of an ion channel in a living cell. The confocal imaging technique, termed FM endocytosis imaging (FEI), can record ligand-GPCR binding with high temporal (second) and spatial (micrometer) resolution. LRB leads to internalization of an endocytic vesicle, which can be labeled by a styryl FM dye and visualized as a fluorescent spo

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22

Gautam, Namrata, Shvetha Sankaran, John A. Yason, Kevin S. W. Tan, and Nicholas R. J. Gascoigne. "A high content imaging flow cytometry approach to study mitochondria in T cells: MitoTracker Green FM dye concentration optimization." Methods 134-135 (February 2018): 11–19. http://dx.doi.org/10.1016/j.ymeth.2017.11.015.

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23

Grigoryev, P. N., G. A. Khisamieva, and A. L. Zefirov. "Septin Polymerization Slows Synaptic Vesicle Recycling in Motor Nerve Endings." Acta Naturae 11, no. 2 (2019): 54–62. http://dx.doi.org/10.32607/20758251-2019-11-2-54-62.

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Septins are GTP-binding proteins recognized as a component of the cytoskeleton. Despite the fact that septins are highly expressed by neurons and can interact with the proteins that participate in synaptic vesicle exocytosis and endocytosis, the role of septins in synaptic transmission and the synaptic vesicle recycling mechanisms is poorly understood. In this study, neurotransmitter release and synaptic vesicle exocytosis and endocytosis were investigated by microelectrode intracellular recording of end-plate potentials and fluorescent confocal microscopy in mouse diaphragm motor nerve ending

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24

Roeder, Amy D., and Janet M. Shaw. "Vacuole Partitioning During Meiotic Division in Yeast." Genetics 144, no. 2 (1996): 445–58. http://dx.doi.org/10.1093/genetics/144.2.445.

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Abstract We have examined the partitioning of the yeast vacuole during meiotic division. In pulse-chase experiments, vacuoles labeled with the lumenal ade2 fluorophore or the membrane-specific dye FM 4-64 were not inherited by haploid spores. Instead, these fluorescent markers were excluded from spores and trapped between the spore cell walls and the ascus. Serial optical sections using a confocal microscope confirmed that spores did not inherit detectable amounts of fluorescently labeled vacuoles. Moreover, indirect immunofluorescence studies established that an endogenous vacuolar membrane p

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25

Somaweera, Himali, Zachary Estlack, Jasmine Pramila Devadhasan, Jungtae Kim, and Jungkyu Kim. "Characterization and Optimization of Isotachophoresis Parameters for Pacific Blue Succinimidyl Ester Dye on a PDMS Microfluidic Chip." Micromachines 11, no. 11 (2020): 951. http://dx.doi.org/10.3390/mi11110951.

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Isotachophoresis (ITP) for Pacific Blue (PB) dye using a polydimethylsiloxane (PDMS) microfluidic chip is developed and characterized by determining the types and concentrations of electrolytes, the ITP duration, and the electric field density. Among candidate buffers for the trailing electrolyte (TE) and leading electrolyte (LE), 40 mM borate buffer (pH 9) and 200 mM trisaminomethane hydrochloride (Tris-HCl) (pH 8) were selected to obtain the maximum preconcentration and resolution of the PB bands, respectively. With the selected TE and LE buffers, further optimization was performed to determ

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Parra, Elisa, Lara H. Moleiro, Ivan López-Montero, Antonio Cruz, Francisco Monroy, and Jesús Pérez-Gil. "A combined action of pulmonary surfactant proteins SP-B and SP-C modulates permeability and dynamics of phospholipid membranes." Biochemical Journal 438, no. 3 (2011): 555–64. http://dx.doi.org/10.1042/bj20110681.

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Proteins SP-B and SP-C are essential to promote formation of surface-active films at the respiratory interface, but their mechanism of action is still under investigation. In the present study we have analysed the effect of the proteins on the accessibility of native, quasi-native and model surfactant membranes to incorporation of the fluorescent probes Nile Red (permeable) and FM 1-43 (impermeable) into membranes. We have also analysed the effect of single or combined proteins on membrane permeation using the soluble fluorescent dye calcein. The fluorescence of FM 1-43 was always higher in me

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27

Basavalingaiah, K. R. "NiO and Ag@NiO Nanomaterials for Enhanced Photocatalytic and Photoluminescence Studies: Green Synthesis Using Lycopodium Linn." Asian Journal of Engineering and Applied Technology 8, no. 2 (2019): 79–85. http://dx.doi.org/10.51983/ajeat-2019.8.2.1133.

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NiO and Ag-NiO nano particles were prepared using Lycopodium extract as a fuel via solution combustion synthesis (SCS) at 500 ºC. From the PXRD, FTIR, UV-DRS studies the synthesized NPs were characterized. The morphologies of the prepared NPs were studied by SEM and TEM analysis. The synthesized NPs were tested for photocatalytic and photoluminescence studies. The PXRD data indicated that the synthesized nanoparticles belong to cubic phase structure and space group Fm-3m. The FTIR spectrum of NiO exhibited the absorption bond at 427 cm-1 is associated to Ni-O vibration band. The SEM data revea

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28

Yip, Kay-Pong. "Flash photolysis of caged nitric oxide inhibits proximal tubular fluid reabsorption in free-flow nephron." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 289, no. 2 (2005): R620—R626. http://dx.doi.org/10.1152/ajpregu.00610.2004.

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A nonobstructing optical method was developed to measure proximal tubular fluid reabsorption in rat nephron at 0.25 Hz. The effects of uncaging luminal nitric oxide (NO) on proximal tubular reabsorption were investigated with this method. Proximal fluid reabsorption rate was calculated as the difference of tubular flow measured simultaneously at two locations (0.8–1.8 mm apart) along a convoluted proximal tubule. Tubular flow was estimated on the basis of the propagating velocity of fluorescent dextran pulses in the lumen. Changes in local tubular flow induced by intratubular perfusion were de

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Matthias, Jessica, Thines Kanagasundaram, Klaus Kopka, and Carsten S. Kramer. "Synthesis of a dihalogenated pyridinyl silicon rhodamine for mitochondrial imaging by a halogen dance rearrangement." Beilstein Journal of Organic Chemistry 15 (October 1, 2019): 2333–43. http://dx.doi.org/10.3762/bjoc.15.226.

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Background: Since their first synthesis, silicon xanthenes and the subsequently developed silicon rhodamines (SiR) gained a lot of attention as attractive fluorescence dyes offering a broad field of application. We aimed for the synthesis of a fluorinable pyridinyl silicon rhodamine for the use in multimodal (PET/OI) medical imaging of mitochondria in cancerous cells. Results: A dihalogenated fluorinatable pyridinyl rhodamine could be successfully synthesized with the high yield of 85% by application of a halogen dance (HD) rearrangement. The near-infrared dye shows a quantum yield of 0.34, co

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30

DE JESUS JEREMIAS, WANDER, JOSE RENAN DA CUNHA MELO, ELIO HIDEO BABA, PAULO MARCOS ZECH COELHO, and JOHN ROBERT KUSEL. "The skin migratory stage of the schistosomulum ofSchistosoma mansonihas a surface showing greater permeability and activity in membrane internalisation than other forms of skin or mechanical schistosomula." Parasitology 142, no. 9 (2015): 1143–51. http://dx.doi.org/10.1017/s0031182015000335.

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SUMMARYSkin schistosomula can be prepared by collecting them after isolated mouse skin have been penetrated by cercariaein vitro. The schistosomula can also migrate out of isolated mouse skin penetrated by cercariaein vitroand from mouse skin penetrated by cercariaein vivo. Schistosomula can also be produced from cercariae applied through a syringe or in a vortex. When certain surface properties of the different forms of schistosomula were compared, those migrating from mouse skin penetrated by cercariaein vivoorin vitrohad greatly increased permeability to membrane impermeant molecules such a

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31

Miyake, K., and P. L. McNeil. "Vesicle accumulation and exocytosis at sites of plasma membrane disruption." Journal of Cell Biology 131, no. 6 (1995): 1737–45. http://dx.doi.org/10.1083/jcb.131.6.1737.

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Plasma membrane disruptions are resealed by an active molecular mechanism thought to be composed, in part, of kinesin, CaM kinase, snap-25, and synaptobrevin. We have used HRP to mark the cytoplasmic site of a mechanically induced plasma membrane disruption. Transmission electron microscopy revealed that vesicles of a variety of sizes rapidly (s) accumulate in large numbers within the cytoplasm surrounding the disruption site and that microvilli-like surface projections overlie this region. Scanning electron microscopy confirmed that tufts of microvilli rapidly appear on wounded cells. Three a

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32

Awin, Labib A., Mahmoud A. El-Rais, Abdunnaser M Etorki, and Moda M. Ezrgane. "Removal of Methyl Violet from Aqueous Solutions Using the A Site Doped Perovskite +-Oxides BaxSr3-xNbO5.5 (x=0, 1 and 2)." Journal of New Developments in Chemistry 3, no. 1 (2020): 23–31. http://dx.doi.org/10.14302/issn.2377-2549.jndc-20-3486.

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Three members of the A- site doped Nb perovskites with general formula Sr3NbO5.5, BaSr2NbO5.5 and Ba2SrNbO5.5 were synthesised by solid-state methods and their removal efficiency of Methyl violet from aqueous solutions investigated. The X-ray diffraction measurements demonstrated that the three samples have a faced cubic perovskite-type structure in space group Fm m. The addition of Ba2+ into the A-site of Sr3NbO5.5 has influenced the cell volume, crystal size and density. Subsequently, the removal capacity was also impacted. The crystallite size of the oxides was determined to be less than 82

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33

Peters, Christian G., and Robert Flaumenhaft. "Localization of VAMP Isoforms In Platelets Reveals Separate Granule Populations with Distinct Functions." Blood 116, no. 21 (2010): 2015. http://dx.doi.org/10.1182/blood.v116.21.2015.2015.

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Abstract Abstract 2015 Secretion of granules from platelets is essential for normal platelet activity in hemostasis and thrombosis. Platelet granules have different morphologies and different granule subpopulations contain distinct cargos. Platelet granules not only participate in the release of granule contents, but also appear to function to increase plasma membrane surface area during platelet spreading, morphogenesis, tether formation, and membrane repair. Whether different granule subpopulations mediate these distinct functions of the granule is currently not known. To judge whether speci

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Cummins, James M., Teruhiko Wakayama, and Ryuzo Yanagimachi. "Fate of microinjected sperm components in the mouse oocyte and embryo." Zygote 5, no. 4 (1997): 301–8. http://dx.doi.org/10.1017/s0967199400003889.

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SummaryIntact mouse sperm or mouse sperm tails alone, labelled with MitoTracker Green FM® fluorochrome, were injected into mouse oocytes and the cells cultured in vitro for up to 5 days. The dye stained midpiece mitochondria, the sperm tail coarse fibres and the sperm perforatorium. Intact sperm (or tails injected with separated heads) induced normal embryonic development. The mitochondria could be identified in embryos up to the 4-cell stage, remaining associated with the sperm tail. They largely disappeared by the 8-cell stage, when only a minority of embryos (6/43) could be found with small

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Ryan, Timothy A. "Optical Mesurements of Presynaptic Function: What Keeps Vesicle Traffic Going." Microscopy and Microanalysis 4, S2 (1998): 1020–21. http://dx.doi.org/10.1017/s1431927600025228.

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The nervous system has evolved to make use of a variety of mechanisms that allow information to flow and be processed among a large collection of individual cells. The communication between individual brain cells occurs largely at chemical synapses. In these compartments, chemical messengers are packaged into small vesicles that fuse with the cell membrane upon stimulation, releasing neurotransmitter.. The average total number of synaptic vesicles in a typical central nervous system synapse is only a few hundred and as a result an efficient local recycling mechanism operates in order to replen

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36

Grigoryev, P. N., and A. L. Zefirov. "The Same Synaptic Vesicles Originate Synchronous and Asynchronous Transmitter Release." Acta Naturae 7, no. 3 (2015): 81–88. http://dx.doi.org/10.32607/20758251-2015-7-3-81-88.

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Transmitter release and synaptic vesicle exo- and endocytosis during high-frequency stimulation (20 pulses/s) in the extracellular presence of different bivalent cations (Ca2+, Sr2+ or Ba2+) were studied in frog cutaneous pectoris nerve-muscle preparations. It was shown in electrophysiological experiments that almost only synchronous transmitter release was registered in a Ca2+-containing solution; a high intensity of both synchronous and asynchronous transmitter release was registered in a Sr2+-containing solution, and asynchronous transmitter release almost only was observed in a Ba2+-contai

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37

Alyamani, Baraa J., Omar A. Alsager, and Mohammed Zourob. "Label-Free Fluorescent Aptasensor for Small Targets via Displacement of Groove Bound Curcumin Molecules." Sensors 19, no. 19 (2019): 4181. http://dx.doi.org/10.3390/s19194181.

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Signal transduction based on fluorescence is one of the most common optical aptasensors for small molecules. Sensors with a number of unique features including high sensitivity, low cost, and simple operation can be constructed easily. However, the label-free fluorescent approach is limited to synthetic dyes that bind strongly to the aptamer sequence and result in a diminished sensor operation with high detection limits. In this study, we report the use of curcumin as a fluorescent probe to signal aptamer/small target binding events. A substantial enhancement in curcumin’s fluorescent emission

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38

Kuznetsov, Andrey V., Oleg Mayboroda, Dagmar Kunz, Kirstin Winkler, Walter Schubert, and Wolfram S. Kunz. "Functional Imaging of Mitochondria in Saponin-permeabilized Mice Muscle Fibers." Journal of Cell Biology 140, no. 5 (1998): 1091–99. http://dx.doi.org/10.1083/jcb.140.5.1091.

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Confocal laser-scanning and digital fluorescence imaging microscopy were used to quantify the mitochondrial autofluorescence changes of NAD(P)H and flavoproteins in unfixed saponin-permeabilized myofibers from mice quadriceps muscle tissue. Addition of mitochondrial substrates, ADP, or cyanide led to redox state changes of the mitochondrial NAD system. These changes were detected by ratio imaging of the autofluorescence intensities of fluorescent flavoproteins and NAD(P)H, showing inverse fluorescence behavior. The flavoprotein signal was colocalized with the potentiometric mitochondria-specif

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39

Bhattacharyya, Bula J., Scott M. Wilson, Hosung Jung, and Richard J. Miller. "Altered neurotransmitter release machinery in mice deficient for the deubiquitinating enzyme Usp14." American Journal of Physiology-Cell Physiology 302, no. 4 (2012): C698—C708. http://dx.doi.org/10.1152/ajpcell.00326.2010.

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Homozygous ataxic mice ( ax J) express reduced levels of the deubiquitinating enzyme Usp14. They develop severe tremors by 2–3 wk of age, followed by hindlimb paralysis, and death by 6–8 wk. While changes in the ubiquitin proteasome system often result in the accumulation of ubiquitin protein aggregates and neuronal loss, these pathological markers are not observed in the ax J mice. Instead, defects in neurotransmission were observed in both the central and peripheral nervous systems of ax J mice. We have now identified several new alterations in peripheral neurotransmission in the ax J mice.

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Haller, Thomas, Paul Dietl, Kristian Pfaller, et al. "Fusion pore expansion is a slow, discontinuous, and Ca2+-dependent process regulating secretion from alveolar type II cells." Journal of Cell Biology 155, no. 2 (2001): 279–90. http://dx.doi.org/10.1083/jcb.200102106.

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In alveolar type II cells, the release of surfactant is considerably delayed after the formation of exocytotic fusion pores, suggesting that content dispersal may be limited by fusion pore diameter and subject to regulation at a postfusion level. To address this issue, we used confocal FRAP and N-(3-triethylammoniumpropyl)-4-(4-[dibutylamino]styryl) pyridinium dibromide (FM 1-43), a dye yielding intense localized fluorescence of surfactant when entering the vesicle lumen through the fusion pore (Haller, T., J. Ortmayr, F. Friedrich, H. Volkl, and P. Dietl. 1998. Proc. Natl. Acad. Sci. USA. 95:

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Haller, Thomas, Klaus Auktor, Manfred Frick, Norbert Mair, and Paul Dietl. "Threshold calcium levels for lamellar body exocytosis in type II pneumocytes." American Journal of Physiology-Lung Cellular and Molecular Physiology 277, no. 5 (1999): L893—L900. http://dx.doi.org/10.1152/ajplung.1999.277.5.l893.

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Pulmonary surfactant is secreted via exocytosis of lamellar bodies (LBs) by alveolar type II cells. Here we analyzed the dependence of LB exocytosis on intracellular Ca2+concentration ([Ca2+]i). In fura 2-loaded cells, [Ca2+]iwas selectively elevated by flash photolysis of a cell-permeant caged Ca2+ compound ( o-nitrophenyl EGTA-AM) or by gradually enhancing cellular Ca2+influx. Simultaneously, surfactant secretion by single cells was analyzed with the fluorescent dye FM 1-43, enabling detection of exocytotic events with a high temporal resolution (T. Haller, J. Ortmayr, F. Friedrich, H. Volkl

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Conboy, Michael J., and Martha S. Cyert. "Luv1p/Rki1p/Tcs3p/Vps54p, a Yeast Protein That Localizes to the Late Golgi and Early Endosome, Is Required for Normal Vacuolar Morphology." Molecular Biology of the Cell 11, no. 7 (2000): 2429–43. http://dx.doi.org/10.1091/mbc.11.7.2429.

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We have characterized LUV1/RKI1/TCS3/VPS54, a novel yeast gene required to maintain normal vacuolar morphology. Theluv1 mutant was identified in a genetic screen for mutants requiring the phosphatase calcineurin for vegetative growth.luv1 mutants lack a morphologically intact vacuole and instead accumulate small vesicles that are acidified and contain the vacuolar proteins alkaline phosphatase and carboxypeptidase Y and the vacuolar membrane H+-ATPase. Endocytosis appears qualitatively normal in luv1 mutants, but some portion (28%) of carboxypeptidase Y is secreted. luv1 mutants are sensitive

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Moreau, V., J. M. Galan, G. Devilliers, R. Haguenauer-Tsapis, and B. Winsor. "The yeast actin-related protein Arp2p is required for the internalization step of endocytosis." Molecular Biology of the Cell 8, no. 7 (1997): 1361–75. http://dx.doi.org/10.1091/mbc.8.7.1361.

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The Saccharomyces cerevisiae actin-related protein Arp2p is an essential component of the actin cytoskeleton. We have tested its potential role in the endocytic and exocytic pathways by using a temperature-sensitive allele, arp2-1. The fate of the plasma membrane transporter uracil permease was followed to determine whether Arp2p plays a role in the endocytic pathway. Inhibition of normal endocytosis as revealed by maintenance of active uracil permease at the plasma membrane and strong protection against subsequent vacuolar degradation of the protein were observed in the mutant at the restrict

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Cabral, Pablo D., Nancy J. Hong, and Jeffrey L. Garvin. "ATP mediates flow-induced NO production in thick ascending limbs." American Journal of Physiology-Renal Physiology 303, no. 2 (2012): F194—F200. http://dx.doi.org/10.1152/ajprenal.00504.2011.

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Mechanical stimulation caused by increasing flow induces nucleotide release from many cells. Luminal flow and extracellular ATP stimulate production of nitric oxide (NO) in thick ascending limbs. However, the factors that mediate flow-induced NO production are unknown. We hypothesized that luminal flow stimulates thick ascending limb NO production via ATP. We measured NO in isolated, perfused rat thick ascending limbs using the fluorescent dye DAF FM. The rate of increase in dye fluorescence reflects NO accumulation. Increasing luminal flow from 0 to 20 nl/min stimulated NO production from 17

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Hilgemann, Donald W., and Michael Fine. "Mechanistic analysis of massive endocytosis in relation to functionally defined surface membrane domains." Journal of General Physiology 137, no. 2 (2011): 155–72. http://dx.doi.org/10.1085/jgp.201010470.

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A large fraction of endocytosis in eukaryotic cells occurs without adaptors or dynamins. Here, we present evidence for the involvement of lipid domains in massive endocytosis (MEND) activated by both large Ca transients and amphipathic compounds in baby hamster kidney and HEK293 cells. First, we demonstrate functional coupling of the two MEND types. Ca transients can strongly facilitate detergent-activated MEND. Conversely, an amphipath with dual alkyl chains, ditridecylphthalate, is without effect in the absence of Ca transients but induces MEND to occur within seconds during Ca transients. C

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Meade, Patricia, Robert S. Hoover, Consuelo Plata, et al. "cAMP-dependent activation of the renal-specific Na+-K+-2Cl− cotransporter is mediated by regulation of cotransporter trafficking." American Journal of Physiology-Renal Physiology 284, no. 6 (2003): F1145—F1154. http://dx.doi.org/10.1152/ajprenal.00421.2002.

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The murine apical bumetanide-sensitive Na+-K+-2Cl− cotransporter gene (mBSC1) exhibits two spliced isoform products that differ at the COOH-terminal domain. A long COOH-terminal isoform (L-mBSC1) encodes the Na+-K+-2Cl− cotransporter, and a short isoform (S-mBSC1) exerts a dominant-negative effect on L-mBSC1 cotransporter activity that is abrogated by cAMP. However, the mechanism of this dominant-negative effect was not clear. In this study, we used confocal microscopic analysis of an enhanced green fluorescent protein (EGFP) fusion construct (L-mBSC1-EGFP) expressed to characterize the surfac

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Xiong, Qiu-Ju, Zhuang-Li Hu, Peng-Fei Wu, et al. "Acid-sensing ion channels contribute to the increase in vesicular release from SH-SY5Y cells stimulated by extracellular protons." American Journal of Physiology-Cell Physiology 303, no. 4 (2012): C376—C384. http://dx.doi.org/10.1152/ajpcell.00067.2012.

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Acid-sensing ion channels (ASICs) have been reported to play a role in the neuronal dopamine pathway, but the exact role in neurotransmitter release remains elusive. Human neuroblastoma SH-SY5Y is a dopaminergic neuronal cell line, which can release monoamine neurotransmitters. In this study, the expression of ASICs was identified in SH-SY5Y cells to further explore the role of ASICs in vesicular release stimulated by acid. We gathered evidence that ASICs could be detected in SH-SY5Y cells. In whole cell patch-clamp recording, a rapid decrease in extracellular pH evoked inward currents, which

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Monteith, Gregory R., and Mordecai P. Blaustein. "Heterogeneity of mitochondrial matrix free Ca2+: resolution of Ca2+ dynamics in individual mitochondria in situ." American Journal of Physiology-Cell Physiology 276, no. 5 (1999): C1193—C1204. http://dx.doi.org/10.1152/ajpcell.1999.276.5.c1193.

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The role of mitochondria in Ca2+ homeostasis is controversial. We employed the Ca2+-sensitive dye rhod 2 with novel, high temporal and spatial resolution imaging to evaluate changes in the matrix free Ca2+ concentration of individual mitochondria ([Ca2+]m) in agonist-stimulated, primary cultured aortic myocytes. Stimulation with 10 μM serotonin (5-HT) evoked modest cytosolic Ca2+ transients [cytosolic free Ca2+ concentration ([Ca2+]cyt) <500 nM; measured with fura 2] and triggered contractions in short-term cultured myocytes. However, 5-HT triggered a large mitochondrial rhod 2 signal (indi

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Kucera, Martin. "Play FM." kma - Klinik Management aktuell 17, no. 10 (2012): 56–59. http://dx.doi.org/10.1055/s-0036-1576652.

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Die Aus- und Fortbildung im Facility Management ist oft Learning by Doing statt systematischer Didaktik. Die Berliner Hochschule für Technik und Wirtschaft entwickelt deshalb ein ernsthaftes Computerspiel für angehende Facility-Manager.

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Yablokov, Arthur Gagikovich, Olga Aleksandrovna Bogoslovskaya, Irina Pavlovna Olkhovskaya, and Natalya Nikolaevna Glushchenko. "Application of metal nanoparticles for pre-sowing treatment of spring barley seeds." JULY 2020, no. 14(7):2020 (July 20, 2020): 1140–49. http://dx.doi.org/10.21475/ajcs.20.14.07.p2366.

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This paper presents data on the pre-sowing treatment of spring barley (Hordeum vulgare L.) seeds by polymer coating with metal nanoparticles (NPs) affecting on seed germination and morphometric parameters of seedlings. Metal NPs [Fe (56.0 ± 0.9 nm, phase composition: Fe0 -27.9 ± 2.1%, Fe3O4 - 72.1 ± 3.6%); Zn (60.6 ± 3.7 nm, phase composition: Zn0 - 100%); Cu (65.0 ± 1.2 nm, phase composition: Cu0 - 100%)] were incorporated into a polymer coating about 10 microns thick, consisting of Na-carboxymethyl cellulose (Na-CMC) and polyethylene glycol-400 (PEG-400), with a dye rhodamine 6G (Rh6G). We d

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