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Journal articles on the topic 'Folin-Ciocalteu reagent'

Author: Grafiati

Published: 4 June 2025

Last updated: 15 July 2025

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1

Nikolaeva, Tat'yana Nikolayevna, Petr Vladimirovich Lapshin, and Natal'ya Viktorovna Zagoskina. "METHOD FOR DETERMINING THE TOTAL CONTENT OF PHENOLIC COMPOUNDS IN PLANT EXTRACTS WITH FOLIN-DENIS REAGENT AND FOLIN-CHOCALTEU REAGENT: MODIFICATION AND COMPARISON." chemistry of plant raw material, no. 2 (June 10, 2021): 291–99. http://dx.doi.org/10.14258/jcprm.2021028250.

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A modification of the method for determining the total content of phenolic compounds in plant tissue extracts with the Folin-Denis reagent and the Folin-Ciocalteu reagent has been carried out, allowing to establish the correspondence of the results obtained when using them. The method using the Folin-Denis reagent is adapted for conducting determinations in microvolumes. For the method using the Folin-Ciocalteu reagent, the concentration of the latter (0.4 N, a 5-fold dilution of the standard reagent) and the composition of the reaction mixture were selected, using which the optical densities

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2

Teixeira da Silva, Jaime A. "IS A FAMED BIOCHEMICAL REAGENT NAMED FOLIN-CIOCALTEAU OR FOLIN-CIOCALTEU?" Central Asian Journal of Medical Hypotheses and Ethics 4, no. 4 (2023): 236–40. http://dx.doi.org/10.47316/cajmhe.2023.4.4.07.

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B, Kulkarni Manasi, and Joshi Anagha M. "AN EXPERIMENTAL DESIGN APPROACH FOR OPTIMIZATION OF MODIFIED COLORIMETRIC FIRST-ORDER DERIVATIVE METHOD FOR ESTIMATION OF SERRALYSIN IN BULK AND PHARMACEUTICAL FORMULATION." Asian Journal of Pharmaceutical and Clinical Research 11, no. 9 (2018): 293. http://dx.doi.org/10.22159/ajpcr.2018.v11i9.26999.

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Objective: The aim of the present work is to use experimental design to screen and optimize experimental variables for developing a colorimetric first-order derivative method for determining content of serralysin (SER) using biuret and Folin–Ciocalteu phenol reagent for stable color development. The method is based on the reaction of peptide bond in the protein with Biuret reagent in alkaline medium and further reaction of remaining tryptophan and tyrosine residues with Folin–Ciocalteu Phenol reagent to form a stable blue-colored complex (first-order derivative λmax 620 nm).Materials and Metho

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4

Hong, Jung-Il, Hyun-Jung Kim, and Ji-Yun Kim. "Factors Affecting Reactivity of Various Phenolic Compounds with the Folin-Ciocalteu Reagent." Journal of the Korean Society of Food Science and Nutrition 40, no. 2 (2011): 205–13. http://dx.doi.org/10.3746/jkfn.2011.40.2.205.

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5

Raza, Asad, and Tariq Mahmood Ansari. "Spectrophotometric Determination of Citalopram in Pharmaceuticals Using Folin-Ciocalteu Reagent." Asian Journal of Chemistry 25, no. 16 (2013): 8913–16. http://dx.doi.org/10.14233/ajchem.2013.14907.

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6

Park, Kyung A., Yoo-mi Choi, Smee Kang, Mi-Ri Kim, and Jungil Hong. "Effects of Proteins on the Reactivity of Various Phenolic Compounds with the Folin-Ciocalteu Reagent." Korean Journal of Food Science and Technology 47, no. 3 (2015): 299–305. http://dx.doi.org/10.9721/kjfst.2015.47.3.299.

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7

Hapsari, Annisa Mulia, Masfria Masfria, and Aminah Dalimunthe. "Pengujian Kandungan Total Fenol Ekstrak Etanol Tempuyung (Shoncus arvensis L.)." Talenta Conference Series: Tropical Medicine (TM) 1, no. 1 (2018): 284–90. http://dx.doi.org/10.32734/tm.v1i1.75.

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Daun tempuyung merupakan salah satu tumbuhan obat tradisional Indonesia yang banyak dimanfaatkan untuk pengobatan batu ginjal, anti inflamasi, disentri, radang dan rematik. Tempuyung banyak mengandung senyawa flavonoid yang merupakan golongan fenol terbesar. Penelitian ini dimulai dengan pengumpulan dan persiapan sampel, skrining fitokimia, pembuatan ekstrak etanol, dan pengujian kandungan total fenol dengan metode kolorimetri pereaksi Folin-Ciocalteu menggunakan spektrofotometer UV-Vis dengan asam galat sebagai baku pembanding. Hasil skrining fitokimia menunjukkan daun tempuyung mengandung se

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8

Jabbar Ilaybi, Hakeem, and Mustafa Abdulkadhim Hussien. "Estimating the concentration of total phenols in wastewater using ultrasonic flow injection analysis system." Al-Kufa University Journal for Biology 15, no. 1 (2023): 27–34. http://dx.doi.org/10.36320/ajb/v15.i1.11726.

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Flow injection analysis is can analyze multiple samples, high accuracy, a very fast response time , modern technical devices that are small. This research intended to study the efficiency of ultrasound waves were determined total phenols in wastewater using Folin-Ciocalteu reagent in basic media .The sample or standard phenol solution were injected into a carrier stream to react with Folin-Ciocalteu reagent then expose it to ultrasound waves (46 kHz) which was detected by spectrophotometer at 765 nm. The calibration curve for this method was in the range of (250-2000 ppm ) .The average correla

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9

Ramesh, Pavagada, Kanakapura Basavaiah, and Nagaraju Rajendraprasad. "Sensitive and selective spectrophotometric assay of doxycycline hyclate in pharmaceuticals using Folin-Ciocalteu reagent." Acta Pharmaceutica 60, no. 4 (2010): 445–54. http://dx.doi.org/10.2478/v10007-010-0032-9.

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Sensitive and selective spectrophotometric assay of doxycycline hyclate in pharmaceuticals using Folin-Ciocalteu reagentA spectrophotometric method for the determination of doxycycline (DOX) is described. The method is based on the formation of blue colored chromogen due to reduction of tungstate and/or molybdate in Folin-Ciocalteu (F-C) reagent by DOX in alkaline medium. The colored species has an absorption maximum at 770 nm and the system obeys Beer's law over the concentration range 0.75-12.0 μg mL-1DOX. The apparent molar absorptivity is 2.78 × 104L mol-1cm-1. The limit of quantification

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10

Dóka, Ottó, Gitta Ficzek, Gergely Simon, Zsolt Zsófi, Szabolcs Villangó, and György Végvári. "Quantification of total polyphenol content in wine lees by conventional optical and photoacoustic spectroscopy." OENO One 57, no. 2 (2023): 257–64. http://dx.doi.org/10.20870/oeno-one.2023.57.2.7178.

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The He-Ne laser (632.8 nm) and photoacoustic spectroscopy (PAS), a variant of the photothermal methods, were combined with the Folin-Ciocalteu colorimetry method to determine total phenolics in wine lees. Utilising this method we found that the total polyphenol content of the nine selected wine lees varied from 1305 to 3907 mg/L. The gallic acid equivalent was determined by means of spectrophotometry using 765 nm as the analytical wavelength. The original Folin-Ciocalteu colorimetry assay was modified for the PAS measurements. Since the PAS does not need dilution, the filtration steps and the

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11

Gabriel, A. Agbor, A. Vinson Joe, and E. Donnelly Patrick. "Folin-Ciocalteau Reagent for Polyphenolic Assay." International Journal of Food Science, Nutrition and Dietetics 3, no. 8 (2014): 147–56. https://doi.org/10.19070/2326-3350- 1400028.

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The chemistry of the Folin-Ciocalteu is described and two Folin assays (single and dual reagent) are described for the assay of phenols and polyphenols with respect to experimental detail and critically evaluated for pure compounds and for mixtures (plant extracts). The single reagent method was found to be more precise and sensitive. The problem of interferences in the Folin assay was evaluated for both methods. Interferences for the dual reagent methodology can be eliminated by a solid phase removal of phenols using a commercial resin (Oasys HLB) or polyvinylpyrrolid

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12

Dumitru, Mihaela Gabriela, and Anca Gănescu. "Antioxidant activity of alcoholic extract of Chelidonium Majus L. Flowers." Annals of the University of Craiova Series Chemistry 28, no. 1 (2022): 63–69. http://dx.doi.org/10.52846/aucchem.2022.1.07.

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The aim of this study is to investigate the total phenolic content and antioxidant activity in extract of the plant Chelidonium majus L. The concentration of total phenolic content was determined using the Folin-Ciocalteu´s reagent and obtained value were 56.5 mg Gallic acid equivalents (GAE) g-1. The antioxidant activity was determined in vitro using DPPH reagent and obtained value were 68.7%.

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13

Denisenko, T. A., A. B. Vishnikin, and L. P. Tsiganok. "REACTION FEATURES OF 18-MOLIBDODIPHOSPHATE AND FOLIN-CIOCALTEU REAGENT WITH PHENOLIC COMPOUNDS." Аналитика и контроль 19, no. 3 (2015): 242–51. http://dx.doi.org/10.15826/analitika.2015.19.3.001.

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14

Shafique Ahmad, Abdullah, Nafisur Rahman, and Fakhrul Islam. "Spectrophotometric Determination of Ampicillin, Amoxycillin, and Carbenicillin Using Folin-Ciocalteu Phenol Reagent." Journal of Analytical Chemistry 59, no. 2 (2004): 119–23. http://dx.doi.org/10.1023/b:janc.0000014736.59554.5c.

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15

Pailla, Naveen Reddy, and Bhaskar Kuthati. "Spectrophotometric determination of Clinidipine, Efavirenz, Prazosin drugs by using Folin-Ciocalteu Reagent." International Journal of ChemTech Research 13, no. 3 (2020): 210–17. http://dx.doi.org/10.20902/ijctr.2019.130318.

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For the determination of three drugs namely Cilnidipine (CDP), Efavirenz (EVZ), Prazosin (PRZ), a simple, fast, selective and accurate spectrophotometric method has been outlined. This method is based on the development of blue coloured chromogen due to reduction of tungstate and/ or molybdate in Folin-Ciocalteu reagent by Drug in alkaline medium. The coloured species has an absorption maximum at 730 nm, 745 nm and 770 nm and the Beer’s law obeys over the concentration range of 12-72 μg/mL, 10-70 μg/mL, 3-21 μg/mL respectively for the drugs CDP, EVZ, PRZ. This method has been validated accordi

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16

Jacangelo, Joseph G., V. P. Olivieri, and Kazuyoshi Kawata. "Thiosulfate-dechlorination interference with the folin-ciocalteu reagent method for protein determination." Water Research 21, no. 9 (1987): 1143–44. http://dx.doi.org/10.1016/0043-1354(87)90036-4.

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17

Attard, Everaldo. "A rapid microtitre plate Folin-Ciocalteu method for the assessment of polyphenols." Open Life Sciences 8, no. 1 (2013): 48–53. http://dx.doi.org/10.2478/s11535-012-0107-3.

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AbstractSeveral methods have been described for the determination of phenolic compounds in animal and plant products using the Folin-Ciocalteu (FC) assay. Most of these methods describe the use of this reagent and sodium carbonate in spectrophotometric methods. The macro FC assay was compared with two micro FC assays carried out on a microplate reader. Excellent correlation was obtained among the three assays with a molar extinction coefficient of 5.228±0.187x103 M−1 cm−1. The micro assay may serve as a high throughput method for the rapid determination of polyphenols in various samples.

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18

Singhvi, I., and Anju Goyal. "Visible spectrophotometric estimation of aceclofenac and indapamide from tablets using folin-ciocalteu reagent." Indian Journal of Pharmaceutical Sciences 69, no. 1 (2007): 164. http://dx.doi.org/10.4103/0250-474x.32140.

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19

Ikawa, Miyoshi, Timothy D. Schaper, Catherine A. Dollard, and John J. Sasner. "Utilization of Folin−Ciocalteu Phenol Reagent for the Detection of Certain Nitrogen Compounds." Journal of Agricultural and Food Chemistry 51, no. 7 (2003): 1811–15. http://dx.doi.org/10.1021/jf021099r.

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20

Everette, Jace D., Quinton M. Bryant, Ashlee M. Green, Yvonne A. Abbey, Grant W. Wangila, and Richard B. Walker. "Thorough Study of Reactivity of Various Compound Classes toward the Folin−Ciocalteu Reagent." Journal of Agricultural and Food Chemistry 58, no. 14 (2010): 8139–44. http://dx.doi.org/10.1021/jf1005935.

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21

Singh, Dhruv K., and Gunjan Maheshwari. "Spectrophotometric determination of penicillins in pure and pharmaceutical formulations using Folin-Ciocalteu reagent." Drug Testing and Analysis 2, no. 10 (2010): 503–6. http://dx.doi.org/10.1002/dta.157.

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22

Nofita, Sisminnitah Dewi, Khoirul Ngibad, and Achmad Fathoni Rodli. "Determination of percentage yield and total phenolic content of ethanol extract from purple passion (Passiflora edulis f. edulis Sims) fruit peel." Jurnal Pijar Mipa 17, no. 3 (2022): 309–13. http://dx.doi.org/10.29303/jpm.v17i3.3461.

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Free radicals can trigger various diseases, such as cancer, cardiovascular, arthritis, diabetes, neurological disorders, and aging. Thus, antioxidants are needed to reduce these free radicals. The peel of the purple passion fruit (Passiflora edulis f. edulis Sims) has many phenolic compounds that indicate antioxidant capacity. This study aims to determine the percentage yield and total phenolic contents in the ethanol extract of purple passion fruit peel. Extraction of purple passion fruit peel using the method of maceration with ethanol solvent. Measurement of total phenolic levels in ethanol

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23

Tariq, Mahmood Ansari, and Raza Asad. "Application of Folin-Ciocalteu reagent for the spectrophotometric determination of zolmitriptan in pharmaceutical preparations." Journal of Indian Chemical Society Vol. 90, Dec 2013 (2013): 2181–86. https://doi.org/10.5281/zenodo.5793689.

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Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, 60800, Pakistan <em>E-mail</em> : asadbzu@yahoo.com <em>Manuscript received online 21 December 2012, revised 06 January 2013, accepted 08 January 2013</em> A fast and selective spectrophotometric method for the determination of zolmitriptan in pharmaceutical bulk and dosage forms is described. The method is based on the formation of blue colored chromogen due to reduction of tungstate in Folin-Ciocalteu reagent by zolmitriptan in alkaline medium. The colored species bas an absorption ·maximum at 750 nm and the Beer&#

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24

Pradeep, Mishra, Gupta Alka, and Shah Kamal. "Spectrophotometric determination of ezetimibe in pharmaceutical formulations." Journal of Indian Chemical Society Vol. 84, Sep 2007 (2007): 945–47. https://doi.org/10.5281/zenodo.5827391.

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Department of Pharmaceutical Sciences, Dr. H. S. Gour University, Sagar-470 003, Madhya Pradesh, India IPS College of Pharmacy, Gwalior, Madhya Pradesh, India GLA Institute of Pharmaceutical Research, Mathura, Uttar Pradesh, India <em>Manuscript received 5 June 2007, accepted 14 June 2007</em> Ezetimibe is chemically 1-( 4-fluorophenyi)-3(<em>R</em>)-[3-(4-fluorophenyi)-3(S)-hydroxypropyi)-4(S)-( 4- ydroxyphenyl)- 2-azetidinone. It is a selective cholesterol absorption inhibitor. A simple, sensitive, accurate, rapid and economical spectrophotometric method is developed, which is based on the f

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Nikolaeva, T. N., P. V. Lapshin, and N. V. Zagoskina. "Method for Determining the Total Content of Phenolic Compounds in Plant Extracts with Folin–Denis Reagent and Folin–Ciocalteu Reagent: Modification and Comparison." Russian Journal of Bioorganic Chemistry 48, no. 7 (2022): 1519–25. http://dx.doi.org/10.1134/s1068162022070214.

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26

Nazrul Islam, Sheikh. "Therapeutic Use Of Vegetable Oils As Functional Food In Alleviation Of Experimental Depression." Food Science and Nutrition 6, no. 5 (2020): 1–7. http://dx.doi.org/10.24966/fsn-1076/100082.

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This article reports the analysis of phenol content, antioxidant capacity of the oil of black cumin, flax, sesame, mustard and fenugreek seeds, and garlic extract. The oils with strong antioxidant activity were tested for alleviation of experiment depression. Folin-Ciocalteu reagent was used to determine the phenol content and DPPH (2,2-diphenyl-1-picrylhydrazyl) was employed for the antioxidant activity

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27

Ilaybi, Hakeem Jabbar, and Mustafa Abdulkadhim Hussien. "Flow injection analysis system with smart phone for the determination of total phenolic compounds in wastewater using Folin Ciocalteu reagent." Journal of Kufa for Chemical Sciences 3, no. 1 (2023): 1–10. http://dx.doi.org/10.36329/jkcm/2023/v3.i1.11114.

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Smart phones are playing a big role in daily life and are being employed as supporting hardware in a variety of chemical analysis applications. flow injection analysis method for the rapid, simple, and straightforward determination of total phenols using Folin-Cioclteu reagent .The sample or standard solutions were injection into a carrier stream to react with reagent and detected by spectrophotometer at 765 nm . The experimental conditions such as flow rate of reagent and carrier ,reagent volume ,length of reaction coil and concentration of reagent were optimized .A good linear calibration cu

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Carmona-Hernandez, Juan C., Gonzalo Taborda-Ocampo, and Clara H. González-Correa. "Folin-Ciocalteu Reaction Alternatives for Higher Polyphenol Quantitation in Colombian Passion Fruits." International Journal of Food Science 2021 (January 14, 2021): 1–10. http://dx.doi.org/10.1155/2021/8871301.

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Passiflora edulis Flavicarpa, Passiflora edulis Sims, and Passiflora ligularis Juss are Colombian fruits (passion fruits) of important exportation value. They act efficiently as antioxidants, antifungal, and antimicrobial compounds due to their high polyphenol content. Polyphenols can be quantified by the Folin-Ciocalteu (F-C) reaction. Food matrices, solvent polarity, and several different reacting conditions are critical for the optimum extraction and quantification of polyphenols. Chromatographic identification and quantitation are satisfactory with access to a vast number of reference stan

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Gurning, Kasta, Sifikal Lumbangaol, Risanti F. R. Situmorang, and Saronom Silaban. "Determination of phenolic contents and antioxidant activity test of ethanol extract of Sirih merah (Piper crocatum Ruiz & Pav.) leaves using the DPPH method." Jurnal Pendidikan Kimia 13, no. 2 (2021): 137–42. http://dx.doi.org/10.24114/jpkim.v13i2.26984.

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The research objectives were to identify the secondary metabolite components, total phenolic content and determine the antioxidant activity of the ethanol extract of red betel leaf (Piper crocatum Ruiz & Pav.). The extraction process was carried out by materation using ethanol as a solvent. Determination of total phenolic content was carried out colorimetrically with Folin-Ciocalteu reagent measured at a maximum wavelength of 765 nm. Determination of antioxidant activity using the DPPH method measured by spectrophotometry at a maximum wavelength of 517 nm. The results of phytochemical scre

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Padda, M. S., and D. H. Picha. "ASSESSMENT OF PHENOLIC ACID QUANTITATION METHODOLOGIES FOR SWEETPOTATOES." HortScience 40, no. 3 (2005): 881c—881. http://dx.doi.org/10.21273/hortsci.40.3.881c.

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Phenolic acids are one of several classes of naturally occurring antioxidant compounds found in sweetpotato. Simplified but reliable methodologies were developed to quantitate total and individual phenolic acids in sweetpotato roots. Total phenolic acid content was measured using both Folin-Denis and Folin-Ciocalteu reagents. The Folin-Ciocalteu reagent gave an overestimation of total phenolic acids due to the absorbance of interfering compounds (i.e., reducing sugars and ascorbic acid). The average total phenolic acid content in `Beauregard' sweetpotatoes was 60.9 mg/100 g fresh weight. Indiv

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Sastry, C. S. P., and A. Rama Mohana Rao. "Application of Folin-Ciocalteu reagent for the spectrophotometric determination of some nonsteroidal antiinflammatory agents." Journal of Pharmacological Methods 19, no. 2 (1988): 117–25. http://dx.doi.org/10.1016/0160-5402(88)90032-0.

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Kabir, Mahfuzul, Md Fazlul Karim Tipu, Mohammad Ashraful Islam, et al. "In vitro Study of Antioxidant Potentials, and Thrombolytic Activity of the Leaves of Delonix regia (Family: Fabaceae)." Bangladesh Pharmaceutical Journal 26, no. 1 (2023): 73–78. http://dx.doi.org/10.3329/bpj.v26i1.64221.

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This study was conducted to assess the antioxidant potential and thrombolytic activity of the methanolic extract of the Delonix regia leaves and its partitionates. The methanolic extract was partitioned into four fractions (pet ether, dichloromethane, ethyl acetate, and aqueous) according to polarity. These fractions were subjected to further investigation. The antioxidant potential was assessed by two methods one was by using the Folin-Ciocalteu reagent as oxidant and gallic acid as a standard; and another method was the DPPH assay. In Folin-Ciocalteu reagent using method, all fractions showe

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Komorowski, James, Devon Bernsley, Sarah Sylla, and Sara Perez Ojalvo. "Enhanced Antioxidant Activity of Phycocyanin Oligopeptides." Current Developments in Nutrition 5, Supplement_2 (2021): 336. http://dx.doi.org/10.1093/cdn/nzab037_046.

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Abstract Objectives Phycocyanin, a phytonutrient found in blue-green algae, has various beneficial properties, including potent antioxidant effects. The goal of this study was to investigate the antioxidant potency of a novel phycocyanin oligopeptide (PC-O) compared to phycocyanin (PC). Methods Antioxidant potency of PC-O and PC was measured using two different methods: 1) the Folin-Ciocalteu reaction assay, and 2) the Oxygen Radical Absorbance Capacity (ORAC) 6.0 assay. The Folin-Ciocalteu reaction assay (or total phenolics assay) was run using Folin-Ciocalteu's phenol reagent and sodium carb

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Abramovič, Helena, Blaž Grobin, Nataša Poklar Ulrih, and Blaž Cigić. "Relevance and Standardization of In Vitro Antioxidant Assays: ABTS, DPPH, and Folin–Ciocalteu." Journal of Chemistry 2018 (October 29, 2018): 1–9. http://dx.doi.org/10.1155/2018/4608405.

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Trolox, gallic acid, chlorogenic acid, caffeic acid, catechin, epigallocatechin gallate, and ascorbic acid are antioxidants used as standards for reaction with chromogenic radicals, 2,2-diphenyl-1-picrylhydrazyl (DPPH⋅) and 2,2′-azino-bis-3-ethylbenzotiazolin-6-sulfonic acid (ABTS⋅+), and Folin–Ciocalteu (FC) reagent. The number of exchanged electrons has been analyzed as function of method and solvent. A majority of compounds exchange more electrons in FC assay than in ABTS and DPPH assays. In reaction with chromogenic radicals, the largest number of electrons was exchanged in buffer (pH 7.4)

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Singh, Dhruv, Bhavana Srivastava, and Archana Sahu. "Spectrophotometric determination of ajmaline and brucine by Folin Ciocalteu’s reagent." Journal of the Serbian Chemical Society 68, no. 8-9 (2003): 685–90. http://dx.doi.org/10.2298/jsc0309685s.

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Arapid and simple spectrophotometric procedure is described for the determination of ajmaline and brucine. The method is based on the development of blue colored product due to reduction of tungstate and/or molybdate in Folin Ciocalteu?s reagent (FCR) by ajmaline and brucine in alkaline medium. The color is stable for more than 48 h. The chromogenic reaction has ?max at 540 nm with molar absorptivity 1.64 x 104 and 2.37 x 103 l mol-1 cm-1 in the Beer?s law range 1?8 ?g ml-1 and 10?100 ?g ml-1 for ajmaline and brucine, respectively.

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Ghalib Syukrillah Syahputra, Delladari Mayefis, Tommy Julianto, and Lyna Elvira. "Penetapan Kadar Fenolik Total Ekstrak Daun Jotang (Sphagneticola Trilobata) Dengan Spektrofotometri UV-Vis." JURNAL RISET RUMPUN ILMU KESEHATAN 2, no. 1 (2023): 277–86. http://dx.doi.org/10.55606/jurrikes.v2i1.2669.

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Phenolic compounds are the largest group of compounds that act as natural antioxidants in plants. One of the plants that has the potential as a natural antioxidant is Jotang (Sphagneticola trilobata). This study aims to determine whether jotang leaf extract (Sphagneticola trilobata) has phenolic content and to determine the total phenolic content in jotang leaf extract (Sphagneticola trilobata). Jotang leaves were extracted by maceration method using methanol solvent. The method of determining total phenolic content was carried out using Folin-ciocaltaeu reagent because phenolic compounds can

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MAK, William Chi Keung. "Comparisons between Hydro and Steam Distillation Processes to Extract Prunella Vulgaris Volatile Compounds, and their Anti-oxidative Activities." Journal of Drug Delivery and Therapeutics 13, no. 3 (2023): 51–57. http://dx.doi.org/10.22270/jddt.v13i3.5955.

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Objective The aim is to verify our earlier suggestion which accounted for the dynamics of volatile compounds extraction from the herb Prunella vulgaris (PV) using steam distillation. Then, the anti-oxidative property of PV is explored. Methods Because we suggested earlier that the inefficient extraction using steam distillation was due to the obstruction of a mass of herb in the steam flow path, we used hydro distillation which tried to eliminate this obstacle. We used GC-MS to characterize the volatile compounds extracted, and thus compare the extraction efficiency. Then, treating the c

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38

Ainsworth, Elizabeth A., and Kelly M. Gillespie. "Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent." Nature Protocols 2, no. 4 (2007): 875–77. http://dx.doi.org/10.1038/nprot.2007.102.

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39

Celeste, Mercedes, Consuelo Tomás, Andreu Cladera, José Manuel Estela, and Victor Cerdà. "Enhanced automatic flow-injection determination of the total polyphenol index in wines using Folin-Ciocalteu reagent." Analytica Chimica Acta 269, no. 1 (1992): 21–28. http://dx.doi.org/10.1016/0003-2670(92)85128-s.

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Hudz, Nataliia, Oksana Yezerska, Mariia Shanaida, Sedláčková Vladimira Horčinová, and Piotr P. Wieczorek. "Application of the Folin-Ciocalteu method to the evaluation of Salvia sclarea extracts." Pharmacia 66, no. (4) (2019): 209–15. https://doi.org/10.3897/pharmacia.66.e38976.

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Polyphenols are valuable group of phytoconstituents due to their high antioxidant activity and healing properties. Antioxidant properties of sages are attributed mainly to a high level of phenolic compounds. The aim of the present study was to elaborate an analytical procedure for the evaluation of the content of secondary metabolites of the polyphenol nature in the herb of Clary Sage (Salvia sclarea L.). Four crude extracts of Salvia sclarea herb obtained with different technologies were used to develop an analytical procedure for the total phenolic content (TPC) assay by spectrophotometric m

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Knez, Eliza, Kornelia Kadac-Czapska, and Małgorzata Grembecka. "Evaluation of Spectrophotometric Methods for Assessing Antioxidant Potential in Plant Food Samples—A Critical Approach." Applied Sciences 15, no. 11 (2025): 5925. https://doi.org/10.3390/app15115925.

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Spectrophotometric antioxidant assays can generally be divided into two fundamental categories: single electron transfer (SET)-based assays and hydrogen atom transfer (HAT)-based methods. In SET-based assays, the progression of the electron exchange reaction is determined by the redox potential of the substrates. In contrast, HAT-based methods assess the antioxidant’s ability to transfer a hydrogen atom to a radical, thereby stabilizing it. The objective of this article is to provide a critical evaluation of antioxidant spectrophotometric assays. Assessing the antioxidant potential of food sho

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Qarah, Nagib A. S., Kanakapura Basavaiah, and Sameer A. M. Abdulrahman. "Spectrophotometric determination of ethionamide in pharmaceuticals using Folin–Ciocalteu reagent and iron(III)-ferricyanide as chromogenic agents." Journal of Taibah University for Science 11, no. 5 (2017): 718–28. http://dx.doi.org/10.1016/j.jtusci.2016.07.002.

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Matejić, Jelena, Ana Džamić, Tatjana Mihajilov-Krstev, Vladimir Ranđelović, Zoran Krivošej, and Petar Marin. "Total phenolic content, flavonoid concentration, antioxidant and antimicrobial activity of methanol extracts from three Seseli L. taxa." Open Life Sciences 7, no. 6 (2012): 1116–22. http://dx.doi.org/10.2478/s11535-012-0094-4.

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AbstractThe present study describes the total phenolic content, concentrations of flavonoids and in vitro antioxidant and antimicrobial activity of methanol extracts from Seseli pallasii Besser, S. libanotis (L.) Koch ssp. libanotis and S. libanotis (L.) Koch ssp. intermedium (Rupr.) P. W. Ball, growing wild in Serbia. The total phenolic content in the extracts was determined using Folin-Ciocalteu reagent and their amounts ranged between 84.04 to 87.52 mg GA (gallic acid)/g. The concentrations of flavonoids in the extracts varied from 4.75 to 19.37 mg Qu (quercetin)/g. Antioxidant activity was

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Pratiwi, Rimadani, Shelvy Elizabeth Suherman, Rajkannah A. L. Poongan, Mutakin Mutakin, and Aliya Nur Hasanah. "Design of Optical Sensor Membrane Based on Polymer Poly(methyl methacrylate) for Paracetamol Detection in Traditional Herbal Medicine." International Journal of Analytical Chemistry 2018 (June 6, 2018): 1–8. http://dx.doi.org/10.1155/2018/8918329.

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Generally, regulation states that herbal medicines are remedies containing plants or preparation of plants as active ingredients only. Paracetamol is one of the drugs that is frequently added in herbal medicine to enhance the effect as an analgesic. The government regulation disallows chemical drugs contained in herbal medicine due to the toxic effect of uncontrolled consumption. On this study, the optical sensor membrane from polymer poly(methyl methacrylate) (PMMA) was synthesized by phase inversion method and was used to detect paracetamol in herbal medicine. PMMA was made in three differen

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Stojanović, Gordana, Igor Stojanović, Vesna Stankov-Jovanović, Violeta Mitić, and Danijela Kostić. "Reducing power and radical scavenging activity of four Parmeliaceae species." Open Life Sciences 5, no. 6 (2010): 808–13. http://dx.doi.org/10.2478/s11535-010-0090-5.

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AbstractThe methanol extracts of four Parmeliaceae lichens (Hypogymnia physodes, Evernia prunastri, Flavoparmelia caperata and Parmelia sulcata) were screened for antioxidant properties and total phenol content. The H. physodes extract was the most effective at reducing iron(III) and scavenging 1,1-diphenyl-2-picrylhydrazyl radicals, while the P. sulcata extract was the most effective in reducing molybdenum(VI) in an acidic medium. The E. prunastri and H. physodes extracts contained more Folin-Ciocalteu reagent reactive substances than the F. caperata and P. sulcata extracts. Significant activ

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Rege, A. A., P. R. Juvekar, and A. R. Juvekar. "IN VITRO LIPID PEROXIDATION INHIBITORY AND ANTI-ARTHRITIC ACTIVITIES OF SOME INDIAN MEDICINAL PLANTS." INDIAN DRUGS 49, no. 06 (2012): 31–35. http://dx.doi.org/10.53879/id.49.06.p0031.

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Anti-lipid peroxidation effect of aqueous extracts of Ocimum sanctum, Tinospora cordifolia and Withania somnifera was evaluated against Fe2+-ascorbic acid-induced lipid peroxidation using rat liver mitochondria as model system, whereas, anti-arthritic activity was evaluated by proteinase inhibitory assay. O. sanctum showed potent anti-lipid peroxidation and anti-arthritic activities. T. cordifolia exhibited moderate anti-lipid peroxidation activity, but considerable anti-arthritic activity, whereas, W. somnifera revealed least lipid peroxidation inhibitory activity and considerable anti-arthri

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Mošovská, Silvia, Dominika Nováková, and Michal Kaliňák. "Antioxidant activity of ginger extract and identification of its active components." Acta Chimica Slovaca 8, no. 2 (2015): 115–19. http://dx.doi.org/10.1515/acs-2015-0020.

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Abstract The object of presented study was the evaluation of antioxidant activity of extract from Zingiber officinale. A method for the preparation of the extract was chosen, which enabled to isolate mostly phenolic compounds. According to the measurements with Folin-Ciocalteu reagent, obtained extract comprised total polyphenols (181.41 mgGAE/g of extract) from which flavonoids contributed to 7.8 % (14.15 mgquercetin/g of extract). Nuclear magnetic resonance (NMR) analysis showed that the phenolic ketones were predominant in the extracts of whole phenolic compounds. According to results of tw

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Qonitah, Fadilah, Reni Ariastuti, and Jihan Aulia Kusumasari. "Penentuan Kandungan Fenolik Total Kombinasi Ekstrak Etanol Daun Jeruk Purut (Citrus hystrix) Dan Daun Kelor (Moringa oleifera L.)." Jurnal Sains dan Kesehatan 5, no. 5 (2023): 823–28. http://dx.doi.org/10.25026/jsk.v5i5.2046.

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Kaffir lime leaves and moringa leaves are known to contain phenolic compounds that can have potential as antioxidants. The purpose of this study was to determine the total phenolic content of the combination of ethanol extracts of kaffir lime leaves and moringa leaves. Preparation of extracts was done by maceration method using 95% ethanol. Determination of total phenolic content by colorimetric method using Folin-Ciocalteu reagent and gallic acid as standard. The results showed that the largest total phenolic content in the combination of J:K extract (1:0) was 3.58 ± 0.09 %EAG while the small

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Kosewski, Grzegorz, Justyna Chanaj-Kaczmarek, Krzysztof Dziedzic, et al. "Changes in Polyphenolic Profile and Antioxidant Properties of Selected Raw and Processed Vegetables Under Different Cooking Methods." Applied Sciences 15, no. 9 (2025): 4677. https://doi.org/10.3390/app15094677.

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The polyphenol profile and antioxidant potential in five raw vegetables (beetroot, red cabbage, red pepper, green pepper, kale) were determined after steaming and sous-vide (SV) at different temperatures (80 °C, 85 °C and 90 °C). The total polyphenol content was determined by spectrophotometry using the Folin–Ciocalteu reagent, the antioxidant properties using the DPPH radical, and the polyphenol profile by HPLC–UV–VIS. The sous-vide method at 85 °C resulted in the greatest, smallest losses or the greatest increase in total polyphenol content, whereas the sous-vide method at 80 °C and 85 °C ha

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Mudura, Elena, Teodora Coldea, Carmen Socaciu, et al. "Brown beer vinegar: A potentially functional product based on its phenolic profile and antioxidant activity." Journal of the Serbian Chemical Society 83, no. 1 (2018): 19–30. http://dx.doi.org/10.2298/jsc170803107m.

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The aim of the present study was to create a functional, enriched in polyphenols and free of alcohol product obtained by acetic fermentation of beer. Beer and vinegar were tested first for their phenolic content and antioxidant activity, by the Folin Ciocalteu and the free radical scavenging activity by the 1,1-diphenyl-2-picrylhydrazyl free-radical scavenging assay, respectively. Then, the separation and identification of the 30 phenolic compounds was realized by high-performance liquid chromatography coupled with positive electrospray ionisation and diode array detection (HPLC-DAD?ESI(+)-MS)

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