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Nicolle, Paméla, Kyle A. Williams, Paul Angers, and Karine Pedneault. "Changes in the flavan-3-ol and polysaccharide content during the fermentation of Vitis vinifera Cabernet-Sauvignon and cold-hardy Vitis varieties Frontenac and Frontenac blanc." OENO One 55, no. 1 (March 22, 2021): 337–47. http://dx.doi.org/10.20870/oeno-one.2021.55.1.3695.
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Grape variety has a significant impact on wine flavan-3-ol and polysaccharide profile. The main objective of this work was to study differences in flavan-3-ol and polysaccharide diffusion from grape to wine during the fermentative alcoholic maceration of three Vitis sp. varieties: the cold-hardy hybrid varieties Frontenac and Frontenac blanc, and the V. vinifera variety Cabernet-Sauvignon. Polysaccharides from must and wine were precipitated by ethanol and quantified using the phenol-sulfuric method of Dubois. Flavan-3-ol concentration and profile were analysed by HPLC-FLD. Results showed that wines from Frontenac and Frontenac blanc had less oligomeric and polymeric flavan-3-ols than those from V. vinifera Cabernet-Sauvignon. Wines made from Frontenac also had a higher concentration in total polysaccharides. Preliminary results from GPC/SEC analyses suggested that Frontenac wine had a higher content in mannoproteins and rhamnogalacturonan-2 polysaccharides compared to the other studied varieties. Overall, wines of Frontenac showed the highest content in total polysaccharides, and the lowest content in condensed tannins. As polysaccharides are known to negatively impact wine perceived astringency, these results suggest that significant attention should be given to the polysaccharide composition of cold-hardy cultivars in the context of cold climate wine production. Such knowledge may help winemakers from cold climate areas to improve the winemaking processes and final wine composition when working with cold-hardy Vitis sp. varieties.Knowledge on interspecific hybrid polysaccharide and flavan-3-ol kinetic during the alcoholic fermentative maceration may help the winemakers from cold climate areas to improve winemaking processes and final wine composition.
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Archana, Ganesan, and Muthusamy Sukumar. "Preparation and Characterisation of Starchy Foods by Plant Polymers from Abelmoscus esculentus Agro Waste and Gracilaria corticata - for Commercial Food Applications." Current Nutrition & Food Science 16, no. 9 (November 29, 2020): 1411–20. http://dx.doi.org/10.2174/1573401316666200319140530.
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Objective: The surface modification of high temperature processed potato chips by structurally stable carbohydrate polymer from Abelmoscus esculentus and Gracilaria corticata were studied. The 1% A. esculentus carbohydrate polymer surface coated chips had less coating pick up than the chips coated with a combination of polysaccharide 1% A. esculentus + Gracilaria corticata polysaccharide. Methods: The mucilage polysaccharides were extracted from the A. esculentus and Gracilaria corticate by hot solvent extraction. The mucilage coating solutions were prepared at 1% concentration individually and in combination for coating the potato strips. The proximate analysis and characterisation of edible coated potato chips were investigated. Results and Discussion: The proximate analysis of carbohydrate polysaccharide coated potato chips showed a reduction in terms of fiber, ash, reducing sugar with a corresponding increase in protein content than control snacks without any coating treatment. The instrumental textural analysis revealed that maximum force required puncturing the surface structure of 1% A. esculentus and 1% A. esculentus + Gracilaria corticata was 16.3-14.04N and 16.95-14.17N which was higher than the force required for puncturing the surface structure of control chips (15.4-13.3N). The instrumental color analysis was performed by image analysis technique, and the effect of the color values of the structural polysaccharide on snack surface were expressed as L*a*b* values. Conclusion: The structural stability of both polysaccharides obtained by fracture force was well in agreement with all of the sensory attributes which showed that they could act as structurally stable polymers that could yield the highest crispiness value for the potato chips with better consumer acceptance.
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Li, Shou Hai, Jian Ling Xia, Chun Peng Wang, Mei Li, Kun Huang, Fu Xiang Chu, and Yu Zhi Xu. "The Physical and Chemical Analysis of Carbohydrate Content in Acorn Kernel." Advanced Materials Research 554-556 (July 2012): 2006–12. http://dx.doi.org/10.4028/www.scientific.net/amr.554-556.2006.
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Carbohydrates are the main source which required to maintain energy equilibrium and normal life activities. In acorn kernel, soluble polysaccharides and starch are the main carbohydrate. In order to accurately measure the carbohydrate content of the acorn kernel in Nanjing Area. Dual-wavelength spectrophotometry and anthrone-sulfuric methods were applied to measure the content of amylose, amylopeetin and soluble polysaccharides,respectively.The other two samples were simultaneously measured for comparative analysis.Testing results show that acorn kernel contain about 79.40% of starch, amylopectin content was up to 59.01%, polysaccharide content is 6.53%.The results indicate that acorn kernel was an important raw material for preparing food, feed and starch-based composites.
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Sánchez-Castillo, Claudia P., Hans N. Englyst, Geoffrey J. Hudson, Jose J. Lara, Marı́a de Lourdes Solano, José L. Munguı́a, and W. Philip T. James. "The Non-Starch Polysaccharide Content of Mexican Foods." Journal of Food Composition and Analysis 12, no. 4 (December 1999): 293–314. http://dx.doi.org/10.1006/jfca.1999.0831.
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Anaya-Esparza, Luis Miguel, Zuamí Villagrán-de la Mora, José Martín Ruvalcaba-Gómez, Rafael Romero-Toledo, Teresa Sandoval-Contreras, Selene Aguilera-Aguirre, Efigenia Montalvo-González, and Alejandro Pérez-Larios. "Use of Titanium Dioxide (TiO2) Nanoparticles as Reinforcement Agent of Polysaccharide-Based Materials." Processes 8, no. 11 (November 1, 2020): 1395. http://dx.doi.org/10.3390/pr8111395.
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In recent years, a strong interest has emerged in polysaccharide-hybrid composites and their potential applications, which have interesting functional and technological properties. This review summarizes and discusses the reported advantages and limitations of the functionalization of conventional and nonconventional polysaccharides by adding TiO2 nanoparticles as a reinforcement agent. Their effects on the mechanical, thermal, and UV-barrier properties as well as their water-resistance are discussed. In general, the polysaccharide–TiO2 hybrid materials showed improved physicochemical properties in a TiO2 content-dependent response. It showed antimicrobial activity against bacteria (gram-negative and gram-positive), yeasts, and molds with enhanced UV-protective effects for food and non-food packaging purposes. The reported applications of functionalized polysaccharide–TiO2 composites include photocatalysts (dye removal from aqueous media and water purification), biomedical (wound-healing material, drug delivery systems, biosensor, and tissue engineering), food preservation (fruits and meat), cosmetics (sunscreen and bleaching tooth treatment), textile (cotton fabric self-cleaning), and dye-sensitized solar cells. Furthermore, the polysaccharide–TiO2 showed high biocompatibility without adverse effects on different cell lines, indicating that their use in food, pharmaceutical, and biomedical applications is safe. However, it is necessary to evaluate the structural changes promoted by the storage conditions (time and temperature) on the physicochemical properties of polysaccharide–TiO2 hybrid composites to guarantee their stability during a determined time.
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Nguyễn, Mạnh Khắc, Hòa Từ Nguyễn, Khuê Ngọc Nguyễn, Diễm My Lâm Huỳnh, Du Huy Nguyễn, and Mai Ánh Nguyễn. "Development of an analytical method for determination of carbohydrates in food by gc - fid using chemical derivatization with anhydride acetic acid." Science and Technology Development Journal - Natural Sciences 4, no. 2 (May 18, 2020): First. http://dx.doi.org/10.32508/stdjns.v4i2.874.
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The present research describes a simple and inexpensive derivatization method that uses acetylation to address the challenges associated with the quantification of the ten most common carbohydrates. The derivatization reaction has two periods : (1) The oxime formation of carbohydrates was carried out at 15 minutes, 500 µL of NH2OH 2.5% and 60 ºC and (2) acetylation of carbohydrates was carried out at 45 minutes, 600 µL of AAA and 80ºC. Most of the carbohydrates generate single peaks via chromatographic separation, except fructose, which generates a double peak. The procedure was successfully applied to analyze carbohydrates in some samples including honey, fresh milk, and polysaccharide hydrolyzate. The method validation results had the linear concentration range of carbohydrates at 50-4000 mg/g, the LODs at 20-50 µg/g, the relative standard deviations (% RSDs) of peak area under 5.0 % and the accuracy at 95–115% of recoveries. The method was applied to determine carbohydrate content in raw milk, honey, and hydrolysis polysaccharide extract. The results showed that the honey sample has fructose and glucose content of 65.8% and 33.4%, respectively, while sucrose makes up 0.74% of the total carbohydrate content. The raw milk sample has lactose content of 47.6% of the total carbohydrates. Some rare polysaccharides such as arabinose and xylose were found in the hydrolysis polysaccharide extract from the mushroom sample.
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Marshall, Valerie M., Eoin N. Cowie, and Rod S. Moreton. "Analysis and production of two exopolysaccharides fromLactococcus lactissubsp.cremorisLC330." Journal of Dairy Research 62, no. 4 (November 1995): 621–28. http://dx.doi.org/10.1017/s0022029900031356.
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SUMMARYTwo polysaccharides produced concurrently byLactococcus lactissubsp.cremorisstrain LC330 have been identified. One had a high molecular mass (> 1 × 106Da) and was neutral. The second was smaller (∼ 10000 Da), charged and had a high phosphorus content. Sugar composition also differed. In chemostat culture the neutral polysaccharide was influenced by temperature and by nitrogen limitation. This polysaccharide was branched with terminal galactose moieties and contained galactose, glucose and glucosamine. The phosphopolysaccharide was more complex with glucose, rhamnose, galactose and glucosamine in an approximate ratio of 6:5:4:1.
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Khan, Bilal Muhammad, Li-Xin Zheng, Wajid Khan, Aftab Ali Shah, Yang Liu, and Kit-Leong Cheong. "Antioxidant Potential of Physicochemically Characterized Gracilaria blodgettii Sulfated Polysaccharides." Polymers 13, no. 3 (January 30, 2021): 442. http://dx.doi.org/10.3390/polym13030442.
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Marine rhodophyte polysaccharides have a wide range of described biological properties with nontoxic characteristics, and show great potential in prebiotics and the functional foods industries. However, there is a virtual lack of Gracilaria blodgettii polysaccharides (GBP) profiling and their bioactivities. This study was designed while keeping in view the lack of physical and chemical characterization of GBP. This polysaccharide was also not previously tested for any bioactivities. A linear random coil conformation was observed for GBP, which was found to be a polysaccharide. A significant sulfate (w/w, 9.16%) and 3,6-anhydrogalactose (AHG, w/w, 17.97%) content was found in GBP. The significant difference in its setting (27.33 °C) and melting (64.33 °C) points makes it resistant to increasing heat. This, in turn, points to its utility in industrial scale processing and in enhancing the shelf-life of products under high temperatures. A radical scavenging activity of 19.80%, 25.42% and 8.80% was noted for GBP (3 mg/mL) in 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis (ABTS) and hydroxyl radical (HO) scavenging assays, respectively. Therefore, the findings suggest that Gracilaria blodgettii polysaccharides display a good antioxidant potential and may have potential applications in the functional food industry.
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Pu, Xiu Ying, Heng Rui Wang, Wen Bo Fan, and Shuang Yu. "Preparation of Guiqi Polysaccharide and Antioxidant Activity In Vitro." Advanced Materials Research 834-836 (October 2013): 539–42. http://dx.doi.org/10.4028/www.scientific.net/amr.834-836.539.
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Polysaccharide extracts (ASP, AMP and GQP) were obtained by water extraction and ethyl alcohol deposition method from Angelica sinensis, Astragalus membranaceus and the mixture of Angelicasinensis and Astragalus membranaceus. And the total carbohydrate content of AMP, ASP and GQP was respectively determined to be 63.1%, 48.7% and 76.7% by phenol-sulfuric acid colorimetric method using glucose as the standard. The in vitro antioxidant activities of 3 polysaccharides were evaluated by free radical, superoxide radicals and DPPH free radical scavenging assay, respectively. Among 3 polysaccharides, GQP showed the highest antioxidant activity in vitro at 100-300 μg/mL. These results indicated that the GQP might be useful for developing natural safe antiaging drugs or health food.
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Lieu, M. D., T. T. L. Nguyen, T. H. Nguyen, T. K. T. Dang, and D. G. Do. "Influence of extraction methods on bioactive compounds from Ngoc Linh ginseng callus." Food Research 5, no. 3 (June 20, 2021): 334–41. http://dx.doi.org/10.26656/fr.2017.5(3).590.
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Panax vietnamensis Ha et Grushv or Vietnamese ginseng is a valuable medicinal herb with high economic value in the world. In this study, three target compounds were surveyed as polyphenols, polysaccharides, and saponins to evaluate the effects of factors including microwave, ultrasound, enzyme amylase, and cellulase for the first time. The results of the study showed that with different extraction conditions, the recovery efficiency of polyphenol, polysaccharide, and saponin was also different. With the addition of 1.2% of the enzyme amylase (v/v) in 8 hrs of incubation, the highest total content of polyphenol and polysaccharide was obtained, equivalent to 6.48 mg GAE/g sample and 312.48 mg Glu/g sample. While the total saponin content reached the highest value of 4.60 mg/g sample at 4 hrs of incubation using 0.8% of cellulase enzyme (v/v). The effect of microwave and ultrasound also showed a significant recovery efficiency for the three analyzed compounds. But compared to the use of two enzymes (amylase, cellulase), the efficiency was lower. The use of these extraction techniques was based on the advantages of environmental friendliness, simple operation, lower investment costs, and power saving. At the same time, it can improve the extraction and recovery efficiency as well as the activity of bioactive compounds.
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Karchava, M., I. Berulava, N. Kintsurashvili, and T. Ergemlidze. "Natural fiber - polysaccharide food supplement of antiotoxic action." Bulletin of Science and Practice 5, no. 3 (March 15, 2019): 154–62. http://dx.doi.org/10.33619/2414-2948/40/20.
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The toxicological assessment of meat raw materials on the consumer market in Imereti region was conducted in Georgia. In 45% of the samples, the content of the lead has been estimated at 2.5–3 times more than 0.1 mg/kg. In modern ecological conditions, the natural fibrous polysaccharide composition of antioxidant action has been worked out to increase the toxicological safety of food raw materials in the modern ecological conditions — the ratio of active wheat bran, high molecular pectin and low molecular pectin 15:3:2. The lead sorption ability has been studied by the composition. The technological properties of the supplement are studied. Based on the obtained results, techniques for functional purpose meat products have been developed for children and diabetes patients using these additives.
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Dobrinčić, Ana, Sandra Pedisić, Zoran Zorić, Mladenka Jurin, Marin Roje, Rozelindra Čož-Rakovac, and Verica Dragović-Uzelac. "Microwave Assisted Extraction and Pressurized Liquid Extraction of Sulfated Polysaccharides from Fucus virsoides and Cystoseira barbata." Foods 10, no. 7 (June 25, 2021): 1481. http://dx.doi.org/10.3390/foods10071481.
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Sulfated polysaccharide fucoidan isolated from brown algae shows a wide range of biological activities that are significantly dependent on its chemical composition, which is closely related to the applied technique and extraction parameters. Therefore, the objective of this study was to evaluate the influence of microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) parameters (solvent, temperature, time, and number of cycles) on the Fucus virsoides and Cystoseira barbata polysaccharide yield (%PS) and chemical composition (total sugar, fucose, and sulfate group). The optimal MAE parameters that resulted in the highest polysaccharide extraction from F. virsoides and C. barbata were 0.1 M H2SO4 for 10 min at 80 °C, while the optimal PLE parameters were 0.1 M H2SO4, for two cycles of 15 min at 140 °C. Furthermore, the %PS, chemical structure, molecular properties, and antioxidant activity of the F. virsoides and C. barbata polysaccharide extracts obtained with MAE, PLE, and conventional extraction (CE) performed under previously determinate optimal conditions were compared. PLE resulted in a significantly higher %PS from F. virsoides, while for C. barbata, a similar yield was achieved with CE and PLE, as well as CE and MAE, for both algae. Furthermore, the polysaccharides obtained using PLE had the highest polydispersity index, fucose, and sulfate group content, and the lowest uronic acid content; however their antioxidant activity was lower.
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Zhu, Jiangxiong, Hui Zhou, Junyao Zhang, Fanglan Li, Kang Wei, Xinlin Wei, and Yuanfeng Wang. "Valorization of Polysaccharides Obtained from Dark Tea: Preparation, Physicochemical, Antioxidant, and Hypoglycemic Properties." Foods 10, no. 10 (September 26, 2021): 2276. http://dx.doi.org/10.3390/foods10102276.
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The structure and hypoglycemic activity of tea polysaccharides has been extensively studied, while there are few reports on the characterization and hypoglycemic activity of dark tea polysaccharides. The crude dark tea polysaccharide (CDTPS) was optimally extracted from Fuzhuan dark tea. Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed. The results revealed that the compositions, structural characteristics, and biological properties of the six DTPSs were different. Therein, DTPS-4 and DTPS-6 had looser morphology, faster solubility, and a more stable structure. Additionally, DTPS-4 had the optimum in vitro antioxidant capabilities, and DTPS-6 had the strongest in vitro hypoglycemic capabilities. In addition, a correlation analysis revealed that the molecular weight and uronic acid content were significantly related to their antioxidant and hypoglycemic activities. Our results indicated that DTPS-4 and DTPS-6 could be further developed into functional foods or additives, respectively.
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Schmid, Vera, Antje Trabert, Judith Schäfer, Mirko Bunzel, Heike P. Karbstein, and M. Azad Emin. "Modification of Apple Pomace by Extrusion Processing: Studies on the Composition, Polymer Structures, and Functional Properties." Foods 9, no. 10 (October 1, 2020): 1385. http://dx.doi.org/10.3390/foods9101385.
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By-products of fruit and vegetable processing are an inexpensive and sustainable source of dietary fiber, potentially offering valuable functional properties such as water binding and thickening. Due to these favorable properties, they can be utilized to reformulate widely-consumed foods, e.g., bakery products or beverages. In this study, apple pomace was used as a model system to study whether extrusion technology affects food by-product functionality and thus has the potential to broaden the application of by-products in foods. The effect of the process parameters and the extent of thermo-mechanical treatment on the structural and functional properties of apple pomace were analyzed after extrusion trials using various screw speeds, water contents, and barrel temperatures. Compared to the raw material, apple pomace extruded at Tbarrel = 100 °C, n = 700 min−1 and mH2O = 17% showed an increased water solubility up to 33%. The water absorption increased from 5 to 19 Pa·s and the paste viscosity from 5 to 339 Pa·s by extrusion processing. Analyses of dietary fiber contents and fiber polysaccharide structures revealed that thermo-mechanical stress (n = 700 min−1, mH2O = 22%) increased the content of soluble dietary fiber from 12.5 to 16.7 g/100 g dry matter, and that the harshest conditions even enabled the formation of low-molecular-weight dietary fiber. Arabinans (as neutral rhamnogalacturonan I side chains) appeared to be most sensitive to thermo-mechanical stress, whereas xylans (i.e., a group of minor polysaccharides) were an example of a more stable fiber polysaccharide. Also, the degree of methylation of the pectic polysaccharides was strongly reduced from 50% to 15% when thermo-mechanical stress was applied. Imaging and pore size analysis showed that extrusion processing could disrupt the rigid cell wall macromolecular structure.
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Zhang, Chi, and Xing Ping Liu. "Antioxidant Activity In Vitro of Selenium Polysaccharide of Thlaspi caerulescens L. Rich in Selenium." Advanced Materials Research 518-523 (May 2012): 5518–22. http://dx.doi.org/10.4028/www.scientific.net/amr.518-523.5518.
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The antioxidant capacity of the human body is closely related to its ability of disease resistance and anti-aging. It is a current research focus to find effective anti-oxidants used in medicine, food, health care, beauty make-up from natural plants. In this paper, we studied the content of selenium in selenium polysaccharide and its antioxidant activity in vitro of Thlaspi caerulescens L. produced from Yutangba of Enshi selenium mining area. The double tracts atomic fluorescence spectrometry is used to detect the total selenium content in the sample and that of selenium polysaccharide in the active ingredient of the sample. The salicylic acid method and pyrogallol autoxidation method are used to detect the scavenging activity of selenium polysaccharide on hydroxyl radicals and superoxide anion. Experiments show that the total amount of selenium in Thlaspi caerulescens L. is 708ug.g-1, while the amount of selenium in selenium polysaccharide is 98.1ug.g-1, accounting 13.86% of the total selenium content. Meanwhile, selenium polysaccharide in samples is with significant antioxidant capacity, and it shows a certain dose-effect relationship. Selenium polysaccharide has stronger anti-oxidation ability than pure polysaccharide compared with non-selenium polysaccharide. Therefore, plants rich in selenium are better natural antioxidants. This study will provide the necessary experimental basis for the effective and rational development and utilization of Thlaspi caerulescens L. rich in selenium
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Arenas, Inma, Miguel Ribeiro, Luís Filipe-Ribeiro, Rafael Vilamarim, Elisa Costa, João Siopa, Fernanda Cosme, and Fernando M. Nunes. "Effect of Pre-Fermentative Maceration and Fining Agents on Protein Stability, Macromolecular, and Phenolic Composition of Albariño White Wines: Comparative Efficiency of Chitosan, k-Carrageenan and Bentonite as Heat Stabilisers." Foods 10, no. 3 (March 12, 2021): 608. http://dx.doi.org/10.3390/foods10030608.
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In this work, the effect of pre-fermentative skin maceration (PFSM) on the chemical composition of the macromolecular fraction, polysaccharides and proteins, phenolic compounds, chromatic characteristics, and protein stability of Albariño monovarietal white wines was studied. PFSM increased the extraction of phenolic compounds and polysaccharides and reduced the extraction of pathogenesis-related proteins (PRPs). PFSM wine showed significantly higher protein instability. Sodium and calcium bentonites were used for protein stabilisation of wines obtained with PFSM (+PFSM) and without PFSM (−PFSM), and their efficiencies compared to fungal chitosan (FCH) and k-carrageenan. k-Carrageenan reduced the content of PRPs and the protein instability in both wines, and it was more efficient than sodium and calcium bentonites. FCH was unable to heat stabilise both wines, and PRPs levels remained unaltered. On the other hand, FCH decreased the levels of wine polysaccharides by 60%. Sodium and calcium bentonite also decreased the levels of wine polysaccharides although to a lower extent (16% to 59%). k-Carrageenan did not affect the wine polysaccharide levels. Overall, k-carrageenan is suitable for white wine protein stabilisation, having a more desirable impact on the wine macromolecular fraction than the other fining agents, reducing the levels of the wine PRPs without impacting polysaccharide composition.
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Xu, Ling, Feng Wang, Zhicai Zhang, and Norman Terry. "Optimization of Polysaccharide Production from Cordyceps militaris by Solid-State Fermentation on Rice and Its Antioxidant Activities." Foods 8, no. 11 (November 19, 2019): 590. http://dx.doi.org/10.3390/foods8110590.
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Polysaccharides are an important class of bioactive components of medical mushroom and herbs and are now used as natural drugs or dietary supplements on a global scale. In this paper, we aimed to increase the polysaccharide production of Cordyceps militaris and the antioxidant activities of fermented rice by solid-state fermentation. The media components and culture condition were optimized by orthogonal design and mono-factor tests using rice as the raw material. The optimal media consisted of (g/L): rice (50), fructose (7), glycerin (7), peptone (1), MgCl2 (0.11), VB1 (0.05), VB2 (0.05), CaCl2 (1.5), corn bran (6), and a water–materials ratio of 100%. The fermentation condition was as follows: inoculum volume of 5.5% (v/w), rice weight of 50 g in one bowl with a diameter of 120 mm and a depth of 90 mm, incubation temperature of 26 °C, and incubation time of seven days. Under the optimized condition, the maximal C. militaris polysaccharide content and free radical scavenging ratio were 68.3 mg/g dry substrate and 98.9%, respectively. This study provides a new strategy for the production of healthy food from traditional food.
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Coles, G. D., S. M. Hartunian-Sowa, P. D. Jamieson, A. J. Hay, W. A. Atwell, and R. G. Fulcher. "Environmentally-Induced Variation in Starch and Non-starch Polysaccharide Content in Wheat." Journal of Cereal Science 26, no. 1 (July 1997): 47–54. http://dx.doi.org/10.1006/jcrs.1996.0102.
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Yu, Qiannan, Meijuan Guo, Bin Zhang, Hao Wu, Yan Zhang, and Lantian Zhang. "Analysis of Nutritional Composition in 23 Kinds of Edible Fungi." Journal of Food Quality 2020 (October 26, 2020): 1–9. http://dx.doi.org/10.1155/2020/8821315.
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To explore the differences in the main nutritional composition of 23 kinds of common edible fungi in the market, the moisture, ash, protein, fat, dietary fiber, carbohydrates, polysaccharides, and energy were analyzed using national standard methods. The results showed that the 23 kinds of edible fungi varied greatly in nutritional composition. Based on dry weight, the moisture content was 6.9∼15.5 g/100 g, the ash content ranged from 1.3 to 10.1 g/100 g, the protein content ranged from 8.5 to 36.9 g/100 g, the fat content was 0.5∼3.9 g/100 g, the dietary fiber content was between 14.4∼70.2 g/100 g, the carbohydrate content is 0.5∼37.3 g/100 g, the polysaccharide content was 2.1∼8.3 g/100 g, and the energy is about 751∼1322 100 g/kJ. All the 23 kinds of edible fungi can be regarded as high-protein low-fat foods, which have their own advantages in terms of nutritional value. This study provides reference for people to use edible fungi in a more scientific and reasonable way.
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Li, Haifeng, Ruona Shi, Fei Ding, Hongyu Wang, Wenjing Han, Fangli Ma, Minghua Hu, Chung Wah Ma, and Zebo Huang. "Astragalus Polysaccharide Suppresses 6-Hydroxydopamine-Induced Neurotoxicity in Caenorhabditis elegans." Oxidative Medicine and Cellular Longevity 2016 (2016): 1–10. http://dx.doi.org/10.1155/2016/4856761.
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Astragalus membranaceus is a medicinal plant traditionally used in China for a variety of conditions, including inflammatory and neural diseases. Astragalus polysaccharides are shown to reduce the adverse effect of levodopa which is used to treat Parkinson’s disease (PD). However, the neuroprotective effect of Astragalus polysaccharides per se in PD is lacking. Using Caenorhabditis elegans models, we investigated the protective effect of astragalan, an acidic polysaccharide isolated from A. membranaceus, against the neurotoxicity of 6-hydroxydopamine (6-OHDA), a neurotoxin that can induce parkinsonism. We show that 6-OHDA is able to degenerate dopaminergic neurons and lead to the deficiency of food-sensing behavior and a shorter lifespan in C. elegans. Interestingly, these degenerative symptoms can be attenuated by astragalan treatment. Astragalan is also shown to alleviate oxidative stress through reducing reactive oxygen species level and malondialdehyde content and increasing superoxide dismutase and glutathione peroxidase activities and reduce the expression of proapoptotic gene egl-1 in 6-OHDA-intoxicated nematodes. Further studies reveal that astragalan is capable of elevating the decreased acetylcholinesterase activity induced by 6-OHDA. Together, our results demonstrate that the protective effect of astragalan against 6-OHDA neurotoxicity is likely due to the alleviation of oxidative stress and regulation of apoptosis pathway and cholinergic system and thus provide an important insight into the therapeutic potential of Astragalus polysaccharide in neurodegeneration.
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Kruchinin, Alexandr G., Olga S. Savinova, Olga A. Glazunova, Konstantin V. Moiseenko, Evgeniya Yu Agarkova, and Tatyana V. Fedorova. "Hypotensive and Hepatoprotective Properties of the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate of Whey Proteins." Nutrients 13, no. 3 (March 23, 2021): 1031. http://dx.doi.org/10.3390/nu13031031.
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Whey protein hydrolysates (WPHs) are one of the most promising sources of biofunctional peptides with such beneficial properties as antioxidant, antihypertensive, anti-inflammatory and others. WPHs also could be used as foaming agents for aerated products (e.g., milk shake type drinks). However, WPH alone has a bitter taste and foamed WPH should be stabilized by additional ingredients. Here, we present a composition including WPH and three polysaccharides—pumpkin pectin, sodium alginate and ι-carrageenan—used as foam stabilizers. Polysaccharide content was selected according to foaming, organoleptic antioxidant and angiotensin-I-converting enzyme inhibitory characteristics of the resulted composition. Further, the hypotensive, antioxidant and hepatoprotective properties of the composition were proved by in vivo tests performed in spontaneously hypertensive rats and Wistar rats with CCl4-induced hepatic injury.
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Gerile, Wu Lan, Naranchimeg Dorjpalam, Wanyuan Gui, Liang Xu, and Jinglin Liu. "The study on the antioxidant activity of polysaccharides isolated from Polygonatum odoratum (Mill.) Druce." Mongolian Journal of Chemistry 21, no. 47 (December 31, 2020): 12–18. http://dx.doi.org/10.5564/mjc.v21i47.1154.
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The polysaccharides isolated from Polygonatum odoratum (Mill.) Druce (POPs) by water extraction, after precipitation with ethanol were purified through deproteinization, decolorization, dialysis, and column chromatography leading to a purified polysaccharide (POPs-I) content of 90.7 %. The scavenging of oxygen free radicals and inhibition of lipid peroxidation (LPO) by POPs-I were analyzed using a colorimetric method. The results showed that the hydroxyl radical scavenging ability of the polysaccharides was weaker than that of benzoic acid, but stronger than those of ascorbic acid and mannitol, and that the superoxide anion radical scavenging ability was inferior to those of all three. When the concentration was higher than 1.0 mg/mL, the POPs-I could inhibit LPO by superoxide anion radicals to a certain degree. Therefore, this work suggests that POPs-I are potential antioxidant agents in medicine and functional food.
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Hristov, A. N., T. A. McAllister, and K. J. Cheng. "Effect of diet, digesta processing, freezing and extraction procedure on some polysaccharide-degrading activities of ruminal contents." Canadian Journal of Animal Science 79, no. 1 (March 1, 1999): 73–81. http://dx.doi.org/10.4141/a98-056.
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Three procedures for extracting polysaccharide-degrading enzymes (PDE) from ruminal contents were compared, and the effects of diet, inoculum preparation and freezing of samples on recovery of enzyme activities were evaluated. Four nonlactating cows and four heifers were fed either high-forage (83%, dry matter [DM] basis) or high-grain (91%, DM basis) diets. Three sample types, (1) enriched ruminal fluid; (2) blended enriched ruminal fluid; and (3) whole ruminal content, were analyzed fresh or after having been frozen (−40 °C) for 48 h. Four cell-free preparations of each sample type were assayed: (1) control (no enzyme extraction); and enzymes extracted by (2) sonication; (3) lysozyme; and (4) lysozyme + EDTA. Animals fed a high-grain diet had lower carboxymethylcellulase andxylanase but higher amylase activities in the rumen. Blending ruminal solids with buffer and combining the filtrate with cheese-cloth-filtered ruminal fluid produced higher polysaccharide-degrading activities per unit of DM compared with whole ruminal contents. In lysozyme treatments, freezing inactivated from 22 (amylase) to 52% (xylanase) of the enzyme activities measured in fresh ruminal fluid. Lysozyme treatment resulted in the highest enzyme recovery (from fresh ruminal fluid) of the extraction methods tested. With frozen samples, sonication and lysozyme gave similar results. If a large number of ruminal content samples are to be processed for PDE activities, freezing and subsequent sonication appear to be a practical alternative to extraction by lysozyme. Key words: Rumen, polysaccharide-degrading enzymes, extraction methods
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Zhang, Yuehong, Zhichao Bao, Xiaoyan Ye, Zhaoyang Xie, Kan He, Bill Mergens, Wenjie Li, Mike Yatcilla, and Qunyi Zheng. "Chemical Investigation of Major Constituents in Aloe vera Leaves and Several Commercial Aloe Juice Powders." Journal of AOAC INTERNATIONAL 101, no. 6 (November 1, 2018): 1741–51. http://dx.doi.org/10.5740/jaoacint.18-0122.
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Abstract Background: There are a substantial number of papers in the scientific literature reporting on the chemical composition of the Aloe vera plant. None of these investigations are truly comprehensive nor address the differences in composition that occur through processing variations in fresh leaves and commercially available product forms. Objectives: This work was to analytically examine a range of these forms and compile the findings. Methods: Fresh A. vera leaves and a number of commercial aloe juice powders were investigated for their major chemical constituents. Samples included fresh leaves from China and Mexico, plus commercial powders from different manufacturers made from different plant parts and/or manufacturing processes. The test results include moisture, ash, fiber, protein, lipids, minerals, organic acids, free sugars, and polysaccharides. The analytical methods employed comprise inductively coupled plasma-optical emission spectroscopy for minerals, high-performance anion-exchange chromatography equipped with pulsed amperometric detection for free sugars, HPLC for organic acids, and size exclusion chromatography (SEC)–multi-angle laser light scattering (MALS)–differential refractive index (dRI) for polysaccharide analyses. The absolute MW and MW distribution were determined using MALS measurement. Results: The major constituents of A. vera fresh leaf are fibers, proteins, organic acids, minerals, monosaccharides, and polysaccharides, which accounted for 85–95% of the total composition determined. In the commercial powdered aloe juice samples, four major components—organic acids, minerals, monosaccharides, and polysaccharides—accounted for 78–84% of the total composition. Apart from the four major components, products manufactured by ethanol precipitation contained high amounts of fiber and protein, while the free sugars were removed. In ethanol-precipitated products, the polysaccharide MW was less affected by manufacturing conditions and the concentration of aloe polysaccharides was higher than in products made in the nonethanol manufacturing processes. The overall chemical profiles were found to be consistent, except for the MW and content of polysaccharides in the commercial aloe samples analyzed, which were largely dependent on the types of manufacturing processes employed. Conclusions: This present study provides a comprehensive investigation of the major chemical composition of A. vera leaf and commercially derived products. The use of the SEC combined with MALS and differential RI detectors has proved to be an improved tool for the accurate determination of polysaccharide MW and contents of the various commercially available A. vera products in this study.
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Osano, Joenalyn P., Seyed H. Hosseini-Parvar, Lara Matia-Merino, and Matt Golding. "Emulsifying properties of a novel polysaccharide extracted from basil seed (Ocimum bacilicum L.): Effect of polysaccharide and protein content." Food Hydrocolloids 37 (June 2014): 40–48. http://dx.doi.org/10.1016/j.foodhyd.2013.09.008.
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Coreta-Gomes, Filipe Manuel, Guido R. Lopes, Cláudia P. Passos, Inês M. Vaz, Fernanda Machado, Carlos F. G. C. Geraldes, Maria João Moreno, Laura Nyström, and Manuel A. Coimbra. "In Vitro Hypocholesterolemic Effect of Coffee Compounds." Nutrients 12, no. 2 (February 9, 2020): 437. http://dx.doi.org/10.3390/nu12020437.
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(1) Background: Cholesterol bioaccessibility is an indicator of cholesterol that is available for absorption and therefore can be a measure of hypocholesterolemic potential. In this work, the effect of commercial espresso coffee and coffee extracts on cholesterol solubility are studied in an in vitro model composed by glycodeoxycholic bile salt, as a measure of its bioaccessibility. (2) Methods: Polysaccharide extracts from coffees obtained with different extraction conditions were purified by selective precipitation with ethanol, and their sugars content were characterized by GC-FID. Hexane extraction allowed us to obtain the coffee lipids. Espresso coffee samples and extracts were tested regarding their concentration dependence on the solubility of labeled 13C-4 cholesterol by bile salt micelles, using quantitative 13C NMR. (3) Results and Discussion: Espresso coffee and coffee extracts were rich in polysaccharides, mainly arabinogalactans and galactomannans. These polysaccharides decrease cholesterol solubility and, simultaneously, the bile salts’ concentration. Coffee lipid extracts were also found to decrease cholesterol solubility, although not affecting bile salt concentration. (4) Conclusions: Coffee soluble fiber, composed by the arabinogalactans and galactomannans, showed to sequester bile salts from the solution, leading to a decrease in cholesterol bioaccessibility. Coffee lipids also decrease cholesterol bioaccessibility, although the mechanism of action identified is the co-solubilization in the bile salt micelles. The effect of both polysaccharides and lipids showed to be additive, representing the overall effect observed in a typical espresso coffee. The effect of polysaccharides and lipids on cholesterol bioaccessibility should be accounted on the formulation of hypocholesterolemic food ingredients.
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GOLUBOWSKA, G. "Changes of polysaccharide content and texture of potato during French fries production." Food Chemistry 90, no. 4 (May 2005): 847–51. http://dx.doi.org/10.1016/j.foodchem.2004.05.032.
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Suwita, I. Komang, and Juin Hadisuyitno. "Mutu gizi dan daya terima es krim indeks glikemik rendah berbahan polisakarida larut air umbi gembili (Dioscorea esculenta) dan tepung ubi jalar ungu (Ipomoea Batatas L. Poir)." Teknologi Pangan : Media Informasi dan Komunikasi Ilmiah Teknologi Pertanian 12, no. 1 (February 27, 2021): 79–91. http://dx.doi.org/10.35891/tp.v12i1.2226.
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Background: The development of ice cream products by adding certain ingredients such as fiber can improve the quality of the product as a healthy food. Research pur¬poses: This study aims to analyze the effect of the water-soluble polysaccharide substi¬tution of gembili tubers with purple sweet potato flour on nutritional quality, physico-chemical properties, and organoleptic quality of ice cream. Method: This study used a completely randomized design (CRD), consists of four levels, namely control (P0) and water-soluble polysaccharide substitution treatment of gembili tubers with purple sweet potato flour; P1, P2, P3 with three replications each. Results and Discussion: The results of the research on ice cream substitution of water-soluble polysaccharide of gembili tubers with purple sweet potato flour showed a significant effect (P <0.05) on ash, protein, and carbohydrate content, and there was no significant effect on mois¬ture content and energy value. Caused increase in the volume of ice cream products at all levels of substitution treatment (P0, P1, P2, and P3). P0 was the ice cream prod¬uct with the highest increase (2,057 times, and then P3 (1,933 times). In the treatment of ice cream, water-soluble polysaccharide substitution of gembili tubers with purple sweet potato flour, there was a change in the ash, protein, fat, carbohydrate content. energy and increased fiber content. Conclusion: The best level of treatment based on the effectiveness index is P3.
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Ghazihoseini, Seyedehzahra, Niloofar Alipoormazandarani, and Abdorreza Mohammadi Nafchi. "The Effects of Nano-SiO2 on Mechanical, Barrier, and Moisture Sorption Isotherm Models of Novel Soluble Soybean Polysaccharide Films." International Journal of Food Engineering 11, no. 6 (December 1, 2015): 833–40. http://dx.doi.org/10.1515/ijfe-2015-0148.
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In this research, a novel polysaccharide-based bionanocomposite film was prepared via dispersion casting method. Nano-silicon dioxide (SiO2-N) was incorporated into soluble soybean polysaccharide (SSPS) at different concentrations (i.e., 0%, 1%, 3%, and 5% w/w dried SSPS). Mechanical (tensile strength, elongation at break, and Young’s modulus), physical (moisture content, water solubility, and moisture uptake), and barrier properties of bionanocomposite films were evaluated. Incorporation of 5% SiO2-N to SSPS matrix decreased water vapor permeability (WVP) from 7.96×10−11 to 4.75×10−11 g m−1 s−1 Pa−1 and oxygen permeability from 215 to 96 cm3 µm m−2 day−1 atm−1. Heat seal strength and mechanical properties of SSPS films were improved. Moisture sorption isotherm of SSPS films supported by SiO2-N was shifted to lower moisture content, and monolayer moisture content of the films decreased significantly (p<0.05). In summary, SiO2-N is a potential filler in SSPS-based films for packaging materials.
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Lin, Li-mei, Ling-jia Zhao, Jing Deng, Su-hui Xiong, Jie Tang, Ya-mei Li, Bo-hou Xia, and Duan-fang Liao. "Enzymatic Extraction, Purification, and Characterization of Polysaccharides from Penthorum chinense Pursh: Natural Antioxidant and Anti-Inflammatory." BioMed Research International 2018 (November 27, 2018): 1–13. http://dx.doi.org/10.1155/2018/3486864.
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Penthorum chinense Pursh (PCP) is a kind of functional food or medicine for liver protection. In the present work, Plackett-Burman design, steepest ascent method, and response surface methodology (RSM) were employed to obtain maximum total sugar yield. The experimental yield of 6.91% indicated a close agreement with the predicted yield of 7.00% of the model under optimized conditions. The major polysaccharide fraction (PCPP-1a) from PCPP was purified and identified as acidic polysaccharides with a high content of uronic acid (FT-IR, UV, HPGPC). PCPP had similar monosaccharide profile with PCPP-1a but was rich in galacturonic acid (HPLC). Both of PCPP and PCPP-1a possessed strong hydroxyl radical scavenging, DPPH radical scavenging, and Fe2+ chelating activities. Moreover, they were revealed to show strong anti-inflammatory activities by inhibiting NO, TNF-α, and IL-1β release compared to LPS treatment in RAW264.7 cells. These data suggest that the polysaccharides from PCP could be potential natural products for treating ROS and inflammatory-related diseases.
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Yan, Xun. "Short-Chain Polysaccharide Analysis in Ethanol–Water Solutions." Journal of AOAC INTERNATIONAL 100, no. 4 (July 1, 2017): 1134–36. http://dx.doi.org/10.5740/jaoacint.16-0426.
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Abstract This study demonstrates that short-chain polysaccharides, or oligosaccharides, could be sufficiently separated with hydrophilic interaction LC (HILIC) conditions and quantified by evaporative light-scattering detection (ELSD). The multianalyte calibration approach improved the efficiency of calibrating the nonlinear detector response. The method allowed easy quantification of short-chain carbohydrates. Using the HILIC method, the oligosaccharide solubility and its profile in water/alcohol solutions at room temperature were able to be quantified. The results showed that the polysaccharide solubility in ethanol–water solutions decreased as ethanol content increased. The results also showed oligosaccharides to have minimal solubility in pure ethanol. In a saturated maltodextrin ethanol (80%) solution, oligosaccharide components with a degree of polymerization >12 were practically insoluble and contributed less than 0.2% to the total solute dry weight. The HILIC–ELSD method allows for the identification and quantification of low-MW carbohydrates individually and served as an alternative method to current gel permeation chromatography procedures.
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Freitas, Mírian Luisa Faria, Ana Paula Badan Ribeiro, and Vânia Regina Nicoletti. "Buriti Oil Emulsions as Affected by Soy Protein Isolate/High-Methoxyl Pectin Ratio, Oil Content and Homogenization Pressure." Food technology and biotechnology 58, no. 2 (July 31, 2020): 159–72. http://dx.doi.org/10.17113/ftb.58.02.20.6210.
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Research background. Emulsion technology is a suitable way of encapsulating, protecting and releasing hydrophobic bioactive compounds for application in food industries, but they are thermodynamically unstable systems. Good results have been achieved for emulsions stabilized by protein-polysaccharide complexes subjected to high-pressure homogenization. Improved stabilization of oil-in-water emulsions results from electrostatic complexes formed between proteins and polysaccharides at pH lower than the protein isoelectric point, which adsorb at the oil-water interface. In addition, polysaccharides contribute to emulsion stability by increasing viscosity of the continuous phase. The aim of this work is to investigate the production of carotenoid-rich buriti oil emulsions using soy protein isolate and high-methoxyl pectin as stabilizers. Experimental approach. Using a rotatable central composite experimental design, we assessed the effects of oil content, soy protein isolate/high-methoxyl pectin ratio and homogenization pressure on the stability, droplet size, electrical conductivity, electrical charge, microstructure and rheological behaviour of the emulsions. Results and conclusions. An optimized emulsion was produced with 28 % buriti oil, 55 % soy protein isolate, and homogenization pressure of 380·105 Pa. This emulsion was stable for at least seven days, presenting reduced average droplet size, low electrical conductivity and high modulus of negative charges. The mechanical spectra showed that the emulsion behaved as a viscoelastic gel under oscillatory, non-destructive shearing, whereas shearthinning behaviour took place under steady shear conditions. Novelty and scientific contribution. The optimized buriti oil emulsions stabilized by soy protein isolate and high-methoxyl pectin could be suitable for fat substitution, energy reduction and carotenoid enrichment in food products, such as dairy and bakery products, ice cream, salad sauces and vegetable-based cream.
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Zhou, Mingming, Yuan Lin, Shengzuo Fang, Yang Liu, and Xulan Shang. "Phytochemical content and antioxidant activity in aqueous extracts of Cyclocarya paliurus leaves collected from different populations." PeerJ 7 (February 19, 2019): e6492. http://dx.doi.org/10.7717/peerj.6492.
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Cyclocarya paliurus (Batal) Iljinskaja is a multiple function tree species, but its main utilization is for the harvesting of its leaves as materials for tea production and recently as ingredients for the food industry. In this study aqueous extracts of C. paliurus leaves collected from 21 natural populations were evaluated for their phytochemical content and antioxidant activity. The content of water-soluble polysaccharide, total flavonoid and total polyphenol varied from 66.05 to 153.32 mg/g, 9.01 to 19.65 mg/g and 20.80 to 52.69 mg/g, respectively. Quercetin-3-O-glucuronide, aemferol-3-O-glucuronide and 3-O-caffeoylquinic acid were the major phenolic components in aqueous extracts of C. paliurus leaves. Both redundancy analysis and Pearson's correlation analysis indicated that water-soluble polysaccharide, total polyphenol, total flavonoid, quercetin-3-O-glucuronide, 3-O-caffeoylquinic acid and 4-O-caffeoylquinic acid were significantly correlated with antioxidant activity, but total polyphenol showed the greatest contribution to antioxidant capacity. The antioxidant activity of the 21 populations was classified into six distinct groups based on the squared Euclidean distance. These results would provide a theoretical basis for obtaining the greatest yield of targeted antioxidant phytochemicals of C. paliurus leaves for tea and food ingredient production.
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Zijlstra, R. T., C. F. M. De Lange, and J. F. Patience. "Nutritional value of wheat for growing pigs: chemical composition and digestible energy content." Canadian Journal of Animal Science 79, no. 2 (June 1, 1999): 187–94. http://dx.doi.org/10.4141/a98-103.
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Nutritional value of cereal grains varies greatly, and is currently estimated foremost by measuring density, whereas prediction based on chemical composition might be more useful. Thus, 16 samples of wheat were evaluated for density, chemical composition, and digestibility in growing pigs. The chemical evaluation included analyses for crude protein (CP), amino acids, neutral detergent fibre (NDF), acid detergent fibre (ADF), starch, and monomer sugars in non-starch polysaccharides (NSP). Digestible energy (DE) content was determined with growing pigs (barrows; 40.8 kg ± 5.3). Density ranged from 57.8 to 77.6 kg hL−1, CP concentration ranged from 13.0 to 18.1%, NDF concentration ranged from 12.9 to 25.0%, NSP concentration ranged from 10.5 to 16.6%, xylose concentration ranged from 4.3 to 6.5%, and DE contentranged from 3701 to 4050 kcal kg−1 Results of chemical analyses were expressed on a dry matter basis. Of the characteristics, xylose had the highest correlation with DE (r = −0.78; P < 0.001), and thus was the best single indicator for DE content (R2 = 0.61; P < 0.001), followed by total NSP (R2 = 0.54), and CP (R2 = 0.49). Density did not predict DE content as well (R2 = 0.43). Using two chemical characteristics, CP and NDF together resulted in the best prediction of DE content (R2 = 0.75; P < 0.001). The results indicate that prediction of nutritional value is more accurate based on chemical characteristics than based on density. Key words: Wheat, digestible energy, growing pig, non-starch polysaccharide
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Zduńczyk, Zenon, Jan Jankowski, Jerzy Juśkiewicz, Dariusz Mikulski, and Bogdan A. Slominski. "Effect of different dietary levels of low-glucosinolate rapeseed (canola) meal and non-starch polysaccharide-degrading enzymes on growth performance and gut physiology of growing turkeys." Canadian Journal of Animal Science 93, no. 3 (September 2013): 353–62. http://dx.doi.org/10.4141/cjas2012-085.
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Zduńczyk, Z., Jankowski, J., Juśkiewicz, J., Mikulski, D. and Slominski, B. A. 2013. Effect of different dietary levels of low-glucosinolate rapeseed (canola) meal and non-starch polysaccharide-degrading enzymes on growth performance and gut physiology of growing turkeys. Can. J. Anim. Sci. 93: 353–362. One-day-old male turkey poults were randomly assigned to eight dietary treatments and were fed diets containing 0, 60, 120, or 180 g kg−1of low-glucosinolate rapeseed meal (RSM) from 1 to 56 d of age without or with a commercial non-starch polysaccharide-degrading enzyme product containing pectinase, cellulase, xylanase, glucanase, mannanase and galactanase activities. There were no significant differences (P>0.05) in body weight and feed conversion ratio between the Control soybean meal-based diet and the diets containing RSM. Overall, the inclusion of up to 180 g kg−1of RSM did not affect the growth performance of turkeys. When compared with the Control treatment, the diet with the lowest RSM content increased the pH of the small intestinal contents and the amounts of cecal digesta. The moderate level of RSM also increased the pH of the small intestinal contents although no increase in the cecal digesta contents was noted. In comparison with other treatments, the highest RSM content increased the content of the small intestinal digesta considerably and decreased the pH of the small intestinal contents to that of the Control treatment. All three dietary levels of RSM significantly reduced the activity of bacterial β-glucuronidase in the ceca. Enzyme supplementation tended to reduce ileal viscosity (P=0.079), decreased ammonia concentration, increased the glycolytic activities of the intestinal microflora enzymes α-glucosidase, α-galactosidase, and β-galactosidase, decreased the activity of β-glucuronidase and increased the growth rate of turkeys.
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Petkova, Nadezhda. "CHARACTERIZATION OF INULIN FROM BLACK SALSIFY (SCORZONERA HISPANICA L.) FOR FOOD AND PHARMACEUTICAL PURPOSES." Asian Journal of Pharmaceutical and Clinical Research 11, no. 12 (December 7, 2018): 221. http://dx.doi.org/10.22159/ajpcr.2018.v11i12.28262.
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Objective: The aim of the current study was to evaluate the black salsify (Scorzonera hispanica L.) as a potential source of inulin and to characterize the physicochemical properties of isolated polysaccharide.Methods: The carbohydrate content in its roots and leaves was analyzed by high-performance liquid chromatography with refractive index detection (HPLC-RID) method. Microwave-assisted extraction was performed for isolation of inulin from black salsify roots. The obtained polysaccharide was characterized by HPLC-RID method, HPLC size-exclusion chromatography, and Fourier transformed-infrared spectroscopy. Functional properties as swelling capacity, solubility, and water-holding and oil-holding capacities (OHCs) were also evaluated.Results: Black salsify (S. hispanica L.) roots were evaluated as a rich source of inulin (22% dw) and 1-kestose (6.25 g/100 g dw). The isolated inulin (yield 20%) was characterized with average degree of polymerization 17, with polydispersity index (1.04) that was near to medium-chained inulin. This polysaccharide showed better OHC than water-holding capacity, and it was characterized with swelling capacity 0.5 ml/g sample.Conclusion: For the first time, inulin was isolated from black salsify roots. The chemical characterization of inulin reveals the potential of this plant to be used as a valuable source of this polysaccharide for future application in food technology and pharmaceutical industry for dietary fibers, stabilizer, and coating agent.
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Birhanie, Ziggiju Mesenbet, Aiping Xiao, Dawei Yang, Siqi Huang, Chao Zhang, Lining Zhao, Liangliang Liu, et al. "Polysaccharides, Total Phenolic, and Flavonoid Content from Different Kenaf (Hibiscus cannabinus L.) Genotypes and Their Antioxidants and Antibacterial Properties." Plants 10, no. 9 (September 14, 2021): 1900. http://dx.doi.org/10.3390/plants10091900.
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Kenaf (Hibiscus cannabinus L.) is a valuable plant with a potential health benefit because of its extensive bioactive compounds. Leaf extracts of 33 kenaf genotypes were investigated for their polysaccharide, total phenolic, and flavonoid content. The antioxidant properties were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and ferric reducing antioxidant potential (FRAP) assays. Antimicrobial capacity was also assessed against Staphylococcus aureus and Escherichia coli using a disc diffusion assay. The polysaccharide content varied from 6.45–16.12 mg glucose per g DW. Total phenolic and flavonoid content ranged from 6.03–21.15 mg GAE/g DW and 1.55–9.24 mg RE/g DW, respectively. Similarly, varied values in the range 20.55–79.99% of inhibition by DPPH, 56.28–88.30% of inhibition by ABTS and 1.26–5.08 mmol Fe2+/g DW by FRAP assays were obtained for antioxidants of the genotype extracts. Extracts from CS4 and CS2 genotypes had the highest antioxidant activities. Kenaf leaves exhibited antibacterial activity against Staphylococcus aureus and Escherichia coli. Strong correlation was found between antioxidant activity with polysaccharide (DPPH, r = 0.893; ABTS, r = 0.819; FRAP, r = 0.864) and total phenolic content (DPPH, r = 0.850; ABTS, r = 0.959; FRAP, r = 0.953). The results suggested that the kenaf leaves could be used as a natural antioxidants and antimicrobial in food industries.
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Fialho, Arsénio M., Lígia O. Martins, Marie-Lucie Donval, Jorge H. Leitão, Michael J. Ridout, Andrew J. Jay, Victor J. Morris, and Isabel Sá-Correia. "Structures and Properties of Gellan Polymers Produced bySphingomonas paucimobilis ATCC 31461 from Lactose Compared with Those Produced from Glucose and from Cheese Whey." Applied and Environmental Microbiology 65, no. 6 (June 1, 1999): 2485–91. http://dx.doi.org/10.1128/aem.65.6.2485-2491.1999.
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ABSTRACT The dairy industry produces large quantities of whey as a by-product of cheese production and is increasingly looking for new ways to utilize this waste product. Gellan gum is reliably produced bySphingomonas paucimobilis in growth media containing lactose, a significant component of cheese whey, as a carbon source. We studied and compared polysaccharide biosynthesis by S. paucimobilis ATCC 31461 in media containing glucose, lactose (5 to 30 g/liter), and sweet cheese whey. We found that altering the growth medium can markedly affect the polysaccharide yield, acyl substitution level, polymer rheological properties, and susceptibility to degradation. Depression of gellan production from lactose compared with gellan production from glucose (approximately 30%) did not appear to occur at the level of synthesis of sugar nucleotides, which are the donors of monomers used for biosynthesis of the repetitive tetrasaccharide unit of gellan. The lactose-derived biopolymer had the highest total acyl content; the glucose- and whey-derived gellans had similar total acyl contents but differed markedly in their acetate and glycerate levels. Rheological studies revealed how the functionality of a gellan polysaccharide is affected by changes in the acyl substitution.
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Seo, Yu-Ri, Dinesh K. Patel, Woo-Chul Shin, Wan-Sup Sim, Ok-Hwan Lee, and Ki-Taek Lim. "Structural Elucidation and Immune-Enhancing Effects of Novel Polysaccharide from Grifola frondosa." BioMed Research International 2019 (April 16, 2019): 1–7. http://dx.doi.org/10.1155/2019/7528609.
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Beta-glucan (β-glucan) is a macromolecule structure where glucose unit has bonded through β-glycosidic bond at 1 and 3 positions. It is well known as a natural immunomodulator without exhibiting any side effects via enhancing immunity. Mushroom contains a large amount of β-glucan and it has anticancerous and antioxidant efficacy. Structure and physical properties of β-glucan are highly influenced by the types of mushroom. In particular, Grifola frondosa has β-1, 3 and β-1, 6 bonds in their structure. It has been noted that β-glucan content also depends upon the size of mushroom particles. The exact content of β-glucan and their immunological activity by a particle size of G. frondosa have yet to be fully elucidated. Herein, β-glucan contents were analyzed according to the particle size of leaf mushroom followed by cell activation and immunoactivity analysis. The highest β-glucan content was observed at a particle size of 20-30 μm (27.65 ± 0.30 w/w). All samples showed ~ 103% cell activation compared to the control and greater cell activity was observed at higher concentration. The significant increase in cytokines secretion was observed in the presence of 20-30 μm particle size of G. frondosa compared to the control. This study suggested that 20-30 μm size is the suitable size of G. frondosa that can be used as a health supplement and food additive to act as an immune booster, hypotensive agent, and hypoglycemic agent.
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Reverberi, M., S. Zjalic, A. Ricelli, C. Di Meo, M. Scarpari, C. Fanelli, and A. Fabbri. "Mushrooms versus fungi: natural compounds from Lentinula edodes inhibit aflatoxin biosynthesis by Aspergillus parasiticus." World Mycotoxin Journal 4, no. 3 (August 1, 2011): 217–24. http://dx.doi.org/10.3920/wmj2010.1270.
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Although the strategies routinely adopted to limit production of carcinogenic aflatoxins by Aspergillus parasiticus and Aspergillus flavus in foods and feeds can be quite effective, they are often neither environmentally friendly, nor non-toxic for end users. Polysaccharides and glycoproteins, particularly β-glucans from the basidiomycete Lentinula edodes, already known for their health-promoting effects on animals and humans, have previously demonstrated the ability to inhibit aflatoxin biosynthesis by stimulating the antioxidant defences of the toxigenic fungus. Here the results of a study regarding the influence on polysaccharide production by L. edodes of oxidative stress induced using paraquat (PQ) treatment are reported. Paraquat 0.5 and 1 mM resulted in an enhancement of the expression of the β-glucan synthase gene Lefks1 and a consequent stimulating effect (about 30-35%) on β-glucans production. Moreover, oxidative-stress (PQ) induced polysaccharides have a higher aflatoxin inhibiting capacity in two different strains of A. parasiticus in comparison with non-induced polysaccharides. This more efficient inhibition might be explained by a higher content of β-glucans because 1H-NMR analysis revealed no obvious qualitative differences between PQ-induced and non-induced polysaccharides. The results obtained show promise for improving the quantity and efficiency of L. edodes extracts in order to achieve enhanced control over aflatoxin biosynthesis in foods and feeds using health-promoting and environmentally-friendly compounds.
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NANASOMBAT, Suree, Kanokwan YANSODTHEE, and Ilada JONGJAITED. "Evaluation of Antidiabetic, Antioxidant and Other Phytochemical Properties of Thai Fruits, Vegetables and Some Local Food Plants." Walailak Journal of Science and Technology (WJST) 16, no. 11 (March 1, 2019): 851–66. http://dx.doi.org/10.48048/wjst.2019.3731.
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Antidiabetic, antioxidant, anti-acetylcholinesterase and prebiotic activities, total phenolics and flavonoids of 33 crude ethanolic extracts of Thai local fruits, vegetables and some local food plants were determined. Mangosteen (Garcinia mangostana) fruit peel and Indian gooseberry (Phyllanthus emblica) fruit extracts had highest antioxidant activity. Bamboo grass (Tiliacora triandra) leaf extract had strongest α-amylase inhibitory activity (78.28 % inhibition), while mulberry (Morus alba) fruit extract had strongest α-glucosidase inhibitory activity (59.63 % inhibition). Star cactus (Aloe vera) leaf pulp extract had strongest anti-acetylcholinesteraseVactivity (31.55 % inhibition). Indian gooseberry fruit and mangosteen fruit peel extracts had highest content of total phenolics and flavonoids, respectively. The extract with highest indigestible polysaccharide content was the extract of mangosteen fruit peels (188.62 mg/g extract), while those with relatively high indigestible polysaccharides were the extracts of pineapple fruits (Ananas comosus), lotus seeds (Nelumbo nucifera), black rice grains (Oryza sativa) and pisang mas banana fruits (Musa sapientum). Based on these properties, 5 plant extracts were selected to study for their prebiotic effect on growth and fermentation of Lactobacillus acidophilus, Lactobacillus bulgaricus and Streptococcus thermophilus in yogurt at 42 °C. Addition of lotus seed extract resulted in highest proliferation of these bacteria with 2.24 logCFU/g increase of total lactic acid bacterial counts in yogurt after 24 h fermentation, while addition of black rice grain, pisang mas banana fruit and pineapple fruit extracts caused good growth of these bacteria with 2.12 - 2.19 logCFU/g increase of total counts.
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McSwain, B. S., R. L. Irvine, M. Hausner, and P. A. Wilderer. "Composition and Distribution of Extracellular Polymeric Substances in Aerobic Flocs and Granular Sludge." Applied and Environmental Microbiology 71, no. 2 (February 2005): 1051–57. http://dx.doi.org/10.1128/aem.71.2.1051-1057.2005.
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ABSTRACT Extracellular polymeric substances (EPS) were quantified in flocculent and aerobic granular sludge developed in two sequencing batch reactors with the same shear force but different settling times. Several EPS extraction methods were compared to investigate how different methods affect EPS chemical characterization, and fluorescent stains were used to visualize EPS in intact samples and 20-μm cryosections. Reactor 1 (operated with a 10-min settle) enriched predominantly flocculent sludge with a sludge volume index (SVI) of 120 ± 12 ml g−1, and reactor 2 (2-min settle time) formed compact aerobic granules with an SVI of 50 ± 2 ml g−1. EPS extraction by using a cation-exchange resin showed that proteins were more dominant than polysaccharides in all samples, and the protein content was 50% more in granular EPS than flocculent EPS. NaOH and heat extraction produced a higher protein and polysaccharide content from cell lysis. In situ EPS staining of granules showed that cells and polysaccharides were localized to the outer edge of granules, whereas the center was comprised mostly of proteins. These observations confirm the chemical extraction data and indicate that granule formation and stability are dependent on a noncellular, protein core. The comparison of EPS methods explains how significant cell lysis and contamination by dead biomass leads to different and opposing conclusions.
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Múzquiz de la Garza, Ana Rocío, Mireya Tapia-Salazar, Maribel Maldonado-Muñiz, Julián de la Rosa-Millán, Janet Alejandra Gutiérrez-Uribe, Liliana Santos-Zea, Bertha Alicia Barba-Dávila, Denis Ricque-Marie, and Lucía Elizabeth Cruz-Suárez. "Nutraceutical Potential of Five Mexican Brown Seaweeds." BioMed Research International 2019 (December 19, 2019): 1–15. http://dx.doi.org/10.1155/2019/3795160.
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In search of pharmaceutically active products to control type 2 diabetes, five brown seaweeds (Silvetia compressa, Cystoseira osmundacea, Ecklonia arborea, Pterygophora californica, and Egregia menziesii) from the Northwest Mexican Pacific coast were investigated. Proximate composition and total polyphenol content (TPC) as phloroglucinol equivalents (PGE) were determined for the five seaweed powders and their respective hydroethanolic (1 : 1) extracts. Extracts were screened for their radical scavenging activity (DPPH and ORAC) and glycosidase inhibitory activity. HPLC-DAD, HPLC-MS-TOF, and ATR-FT-IR methodologies were used to identify the most abundant phlorotannins and sulfated polysaccharides in the extracts. Hydroethanolic extracts contained minerals (17 to 59% of the dry matter), proteins (4 to 9%), ethanol-insoluble polysaccharides (5.4 to 53%), nitrogen-free extract (NFE) (24.4 to 70.1%), lipids (5 to 12%), and TPC (2.6 to 47.7 g PGE per 100 g dry extract). S. compressa and E. arborea dry extracts presented the lowest ash content (26 and 17%, respectively) and had some of the highest phenolic (47.7 and 15.2 g PGE per 100 g extract), NFE (57.3 and 70.1%), and soluble polysaccharide (19.7 and 53%) contents. S. compressa and E. arborea extracts had the highest antioxidant activity (IC50 DPPH 1.7 and 3.7 mg mL−1; ORAC 0.817 and 0.801 mmol Trolox equivalent/g extract) and the highest α-amylase and α-glucosidase inhibitory capacities (IC50 940 and 1152 μg mL−1 against α-amylase and 194 and 647 μg mL−1 against α-glucosidase). The most abundant phlorotannins identified in the extracts were phloretol, fucophloroethol, and two- and three-phloroglucinol unit (PGU) phlorotannins. Laminarin, fucoidan, and alginate were among the sulfated polysaccharides identified in the extracts. The bioactivities of S. compressa and E. arborea extracts were mainly related with their contents of three PGU phlorotannins and sulfated polysaccharides (e.g., fucoidan, laminarin, and alginate). These results suggest S. compressa and E. arborea are potential candidates for food products and nutraceutical and pharmaceutical preparations, and as additives for diabetes management.
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Teng, Cong, Zhenxing Shi, Yang Yao, and Guixing Ren. "Structural Characterization of Quinoa Polysaccharide and Its Inhibitory Effects on 3T3-L1 Adipocyte Differentiation." Foods 9, no. 10 (October 21, 2020): 1511. http://dx.doi.org/10.3390/foods9101511.
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Quinoa is a kind of nutritious food crop with anti-obesity activity, however, the mechanism is not unclear. In this study, we separated and purified bioactive polysaccharide from quinoa (denoted SQWP-2). The chemical structural was characterized and its effect on 3T3-L1 pre-adipocyte differentiation was evaluated. The molecular weight of SQWP-2 was found to be 7.49 × 103 Da, and the polysaccharide consisted of fructose and glucose. The Glc-(1→, Fru-(2→, →4)-Glcp-(1→, and →4,6)-Glcp-(1→ glycosidic linkages were identified in SQWP-2 through gas chromatography-mass spectrometry. Nuclear magnetic resonance confirmed the monosaccharide composition and glycosidic linkage content, and a suggestion of the structural formula is provided. In Western Blotting and RT-PCR assays, treatment with SQWP-2 significantly inhibited 3T3-L1 differentiation by suppressing PPARγ, C/EBPα, C/EBPβ, C/EBPδ, SREBP1C and AP2 expression. Quinoa polysaccharide isolated here could represent an anti-obesity agent once the structures and differentiation inhibition are definitively characterized.
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Balli, Diletta, Francesca Tozzi, Mohamad Khatib, Alessandra Adessi, Pablo Melgarejo, Grazia Masciandaro, Edgardo Giordani, Marzia Innocenti, and Nadia Mulinacci. "Purple Queen® fruits of Punica granatum L.: Nutraceutical properties and unconventional growing substrates." Journal of Berry Research 10, no. 4 (December 15, 2020): 637–50. http://dx.doi.org/10.3233/jbr-200546.
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BACKGROUND: Purple Queen® is an early ripening pomegranate cultivar growing well in soilless cultivation. Substrates have direct effects on plant development and, due to progressive peatland exhaustion, the request for new materials for plant growth is high. OBJECTIVE: The objective of this work was to verify the effects of a new potential substrate obtained from the remediation of marine port sediments on the nutraceutical profile of Purple Queen® fruits, using 50% and 100% of reclaimed sediment. METHODS: The study was carried out determining ellagitannins and polysaccharides obtained from peel after decoction, and anthocyanins in aril juice on fruits from two agronomic seasons. RESULTS: The presence of 100% of the sediment had a partial detrimental effect on fruits size and weight. Compared to a peat-based commercial substrate (control), remediated sediment preserved ellagitannin content and increased the anthocyanin amount (up to 40% higher) and polysaccharide content up to 34% on dry fruit. High molecular weight polysaccharides (>2000 kDa) were identified in all the fruits with glucose and galacturonic acid as the major sugars. CONCLUSIONS: Fruits from plants cultivated only on sediment or in a mixture with 50% of peat showed significant increases of bioactive compounds as stress response in two consecutive seasons.
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Theander, Olof, Per Åman, Eric Westerlund, Roger Andersson, and Dan Pettersson. "Total Dietary Fiber Determined as Neutral Sugar Residues, Uronic Acid Residues, and Klason Lignin (The Uppsala Method): Collaborative Study." Journal of AOAC INTERNATIONAL 78, no. 4 (July 1, 1995): 1030–44. http://dx.doi.org/10.1093/jaoac/78.4.1030.
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Abstract A joint AOAC/American Association of Cereal Chemists (AACC) collaborative study was conducted to determine by the Uppsala method the dietary fiber content and its composition in various foods. The method includes preparation of a residue by treatment with thermostable α-amylase and amyloglucosidase and then ethanol precipitation of solubilized dietary fiber components while leaving low-molecular weight carbohydrates in solution. After acid hydrolysis of residue, neutral polysaccharide residues are determined as alditol acetates by gas-liquid chromatography, uronic acid residues are determined by colorimetry, and ash-free acid-insoluble residue (Klason lignin) is determined gravimetrically. Total dietary fiber, including enzyme-resistant starch, is calculated as the sum of nonstarch polysaccharide residues and Klason lignin. Nine laboratories completed the study, analyzing in duplicate 8 unknown dried products that included 4 cereal products, green peas, potato fiber, carrots, and apples. Total dietary fiber contents of products tested ranged from 4.6 to 84.3%, with an average RSDR value of 8.4% (range, 4.8–11.1%). Total neutral polysaccharide residues ranged from 3.8 to 64.1%, with an average RSDR value of 7.5% (range, 5.4–10.5%). Individual neutral sugars (rhamnose, arabinose, xylose, mannose, galactose, and glucose) and uronic acid residues present at more than 1% generally had good RSDR values (3.3– 22.8%), whereas, as expected for Klason lignin, only the wheat bran sample with a high content (16%) had an excellent RSDR value (5.0%). The gas chromatographic-colorimetric-gravimetric method (Uppsala method) for determination of total dietary fiber (as neutral sugar residues, uronic acid residues, and Klason lignin) has been adopted first action by AOAC INTERNATIONAL.
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He, Zongxing, Yulian Zhu, Xingyu Bao, Liuxin Zhang, Nan Li, Gonglingxia Jiang, and Qiang Peng. "Optimization of Alkali Extraction and Properties of Polysaccharides from Ziziphus jujuba cv. Residue." Molecules 24, no. 12 (June 14, 2019): 2221. http://dx.doi.org/10.3390/molecules24122221.
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Ziziphus jujuba cv. Muzao is a plant widely cultivated in the Yellow River Basin of China. It has nutritional and healthcare functions, in which polysaccharides are the main components of its bio-functions. In order to make effective use of Ziziphus jujuba cv. Muzao residue resources and explore new functional food ingredients, the polysaccharide (ZJRP) from Ziziphus jujuba cv. Muzao residues were extracted by sodium hydroxide, and the optimal extraction conditions of ZJRP were obtained by the response surface method. The basic composition and antioxidant effects of ZJRP were determined. The results showed that ZJRP has significant antioxidant activity, mainly reflected in the high DPPH radical scavenging rate, which may be related to their high content of galacturonic acid and the extraction method. In addition, the rheological and thermal properties of ZJRP were respectively determined by a rheometer and differential scanning calorimetry (DSC), indicating that they have shear thinning properties and good thermal stability. Results showed that the alkaline extraction method can be used as a potential technique for extracting ZJRP with high antioxidant activity, and ZJRP can be further explored as a functional food ingredient.
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Cagnin, Caroline, Geovana Rocha Placido, Maisa Dias Cavalcante, Bheatriz Silva Morais de Freitas, Daniel Emanuel Cabral de Oliveira, and Tainara Leal de Sousa. "Utilization of the co-product of jaboticaba (Myrciaria jaboticaba Berg.) for pectin extraction." Research, Society and Development 9, no. 6 (April 10, 2020): e25963453. http://dx.doi.org/10.33448/rsd-v9i6.3453.
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Pectin is a polysaccharide that can be applied to various food industry products to modify the viscosity of food. The objective of this work was to optimize the extraction of pectin of jabuticaba peel by modificating the yield, galacturonic acid content (AUA), esterification content (DE), pectin content (P), and neutral sugars (N). The experiment was performed using the technique of factorial planning of complete experiments and surface response analysis. The extraction can be reported through factorial planning with coefficients of determination (R²) of 0.92, 0.91, 0.89, 0.88 and 0.90 for the yield extraction, AUA, DE, P, and N, respectively. The content of AUA ranged from 22.82 to 65.35%, the DE varied from 26.72 to 77.50%, the percentage of P found in the extraction 25.50 to 66.12%. The best performance was verified utilizing the following parameters: extraction time less than 75 min, temperature of 70°C, and citric acid concentration of 0.75 mol.L-1.
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Thuy, Tran Thi, Nguyen Thi Thu Hoai, and Tong Thi Mo. "Assessment of some nutrient contents and enzyme activity in early germinating bean of two soybean varieties (Glycine max) DT84 and DT2008." TAP CHI SINH HOC 40, no. 1 (January 25, 2018): 76–83. http://dx.doi.org/10.15625/0866-7160/v40n1.10865.
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Soybeans have become widly important and popular due to a high demand of plant protein and fiber. Besides, grains of soybean contain abundance of vitamines, minerals and bioactive compounds such as isoflavone, gama- aminobutyric acid... However, some antinutrient compounds such as tripsin inhibitor and phytate are available in these grains. In this paper, nutrient compounds (protein, lipid, polysaccharide and dissolved minerals) and enzymes of early germinating seeds of soybean varieties (DT84 and DT2008) were evaluated and compared to those of non-germinating beans in order to determine a suitable germinating period for food processing. Experimental results showed that: Activities of enzymes (protease, amylase and phytase) in early germinating soybeans were higher than those in dry beans. Those enzymes have made major changes in nutrient compounds of soybeans: proteins, polysaccharides and lipids reduced. Especially, the content of phytate, an antinutrient factor, reduced signicantly (17.0-48.9% in DT84 variety and 28.0-60.7% in DT2008 variety) during early germinating period of soybeans. However, easy uptake minerals (calcium, iron and zinc ions), reduced sugars and peptides were significantly increased. In detail, the increase in dissolve iron cation was 10.31-12.13%, calcium cation was 31.56-35.75% and zinc cation was 31.38-43.32%. These results confirmed the positive effect of germinating soybeans in food processing.
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Chu, Jiunn-Nan, Chiu-Chung Young, Chen-Chung Tan, Szu-Pei Wu, and Li-Sen Young. "Improvement of productivity and polysaccharide-protein complex in Agaricus blazei." Pesquisa Agropecuária Brasileira 47, no. 1 (January 2012): 96–102. http://dx.doi.org/10.1590/s0100-204x2012000100013.
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The objective of this work was to assess the productivity and polysaccharide-protein complex content of Agaricus blazei on rice straw medium, in comparison to conventional sawdust, using four casing soils. The A. blazei strain used was BCRC36814T, purchased from the Food Industry Research and Development Institute, Hsin-Chu, Taiwan. The two media were evaluated as to A. blazei productivity, harvesting time, and production costs. The experimental design used was a randomized complete block, with four replicates. Three local casing soils - Typic Paleudult (CCe), Typic Udorthent (Tq) and Oxyaquic Paleudult (TSp) - were compared to imported peat soil (PS, Saprists, Histosols), used as the control. The productivity of A. blazei using Tq and TSp soil was significantly higher. The TSp casing treatment resulted in earlier harvest by at least 14 to 27 days, when compared to the other treatments. The polysaccharide content in CCe (13.2%) and Tq soils (13.2%) did not differ significantly from the PS (13.4%) and TSp (10.6%) treatments. Local casing soils decreased the production costs of A. blazei cultivation. Composted rice straw can substitute sawdust as the culture medium for A. blazei production with increased yield.