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Journal articles on the topic 'Formalin alternative'

Author: Grafiati

Published: 7 June 2025

Last updated: 2 August 2025

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1

Gotur, Suhasini P., Nagaraju Kamarthi, and Vijay Wadhwan. "Sirka: An alternative to the formalin fixative." Indian Journal of Pathology and Microbiology 67, no. 1 (2023): 238–41. http://dx.doi.org/10.4103/ijpm.ijpm_510_23.

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ABSTRACT Formalin fixation is the most essential step of routine histopathology practice. During the last few years, various fixatives have been developed for use in histopathology practice as an alternative to formalin, to overcome its side effects on health. Here we have demonstrated an interesting and novel idea of using sirka or sugar cane vinegar as an alternative to the formalin with the adequate result.

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Hachabizwa, Caswell, Mickey Banda, Joseph Hainza, Sikhanyiso Mutemwa, Krikor Erzingastian, and Elliot Kafumukache. "Cadaveric embalming using a modified Thiel method as an alternative to the formalin method." Anatomy Journal of Africa 9, no. 2 (2020): 1797–806. http://dx.doi.org/10.4314/aja.v9i2.199945.

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Formalin embalming method is cheaper and commonly used. Thiel method is said to be complex, expensive, not commonly used but offers better cadavers for student teaching. The study aimed at exploring the effects of two embalming methods; modified Thiel and formalin methods on cadavers in terms of muscle softness, joint flexibility, tissue colour, formalin smell and the ease with which structures can be identified. This was an experimental study design done over the period of three months where four adult male cadavers were embalmed (two under each method) and the outcome was compared. Using gra

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Satapathy, Bikash Chandra, and Biswa Bandita Kar. "Alternative natural and chemical substances to traditional formalin-based embalming fluid for cadaveric dissection: A review." Indian Journal of Clinical Anatomy and Physiology 10, no. 2 (2023): 66–73. http://dx.doi.org/10.18231/j.ijcap.2023.016.

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Formalin, a widely used embalming fluid in the preservation of cadavers, poses significant health risks to anatomists, medical students, and the environment. This review article evaluates alternative natural and chemical substances for embalming fluid in cadaveric dissection. We conducted a comprehensive literature search on PubMed, Scopus, and Web of Science, focusing on alternative embalming fluids in the past 20 years. The main outcome of interest was the effectiveness of alternative fluids in preserving cadavers for dissection. The review identified several alternatives, including Thiel's

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Sumiati. "PURPLE CABBAGE EXTRACTS (Brasicca oleracea L) AS TOFU'S FORMALIN INDICATORS." Integrated Lab Journal 7 (May 2, 2019): 44–55. https://doi.org/10.5281/zenodo.2656806.

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Tofu is a food that is in great demand by people in Indonesia. In processing tofu, producers usually use formalin as a preservative to maintain storage time. The use of formalin in food can cause poisoning. This study aims to determine the method that can be used to unlock the potential of purple cabbage plants (<em>Brasicca oleracea L</em>) as an alternative natural indicator in the qualitative examination of formalin and to obtain experimental results using purple cabbage extract (<em>Brasicca oleracea L</em>) as an alternative natural indicator of deep formalin examination know. Purple cabb

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Shetty, Jayaprakash Kubalady, Hannah Fathima Babu, and Kishan Prasad Hosapatna Laxminarayana. "Histomorphological Assessment of Formalin versus Nonformalin Fixatives in Diagnostic Surgical Pathology." Journal of Laboratory Physicians 12, no. 04 (2020): 271–75. http://dx.doi.org/10.1055/s-0040-1722546.

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Abstract Introduction Fixation is the critical step in the preservation of tissues in diagnostic pathology. The formalin is an economical and excellent fixative with the inherent property of adequate fixation. The well-established side effects of formalin include mucosal irritation, upper respiratory diseases, and corrosive injury to the gastrointestinal tract. In addition, substantial evidence exists regarding the potential role of formaldehyde as a human carcinogen. The carcinogenic and toxic effects of formalin encourage searching for alternative fixatives for tissue fixation. However, “the

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Sachdev, Sanpreet Singh, Tabita Joy Chettiankandy, Manisha Ahire Sardar, Prajwalit Prakash Kende, Rajesh Prabhakar Gaikwad, and Lynette Custodio Fernandes. "Utilizing Almond Oil with Vitamin E as an Alternative Fixative to Formalin." Annals of Pathology and Laboratory Medicine 9, no. 6 (2022): A121–125. http://dx.doi.org/10.21276/apalm.3169.

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Introduction: The health hazards resulting from exposure to formalin have given rise to the need of developing safer alternative fixatives. Almond oil has been widely used in the aromatherapy and cosmetic industry for its good penetrating and skin-rejuvenating properties, whereas vitamin E is a widely known anti-oxidant. The present study aimed to test the fixative efficacy of almond oil with vitamin E as compared to formalin. Material and Methods: The present study was conducted on 42 tissue specimens obtained by crown lengthening procedure or other minor surgical procedures. The specim

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Ali, Riaz, Justin Bartholomew, Randolph Coffey, Caroline Carrico, Todd Kitten, and Parthasarathy Madurantakam. "Peracetic Acid: A Practical Alternative to Formalin for Disinfection of Extracted Human Teeth." Bioengineering 8, no. 12 (2021): 217. http://dx.doi.org/10.3390/bioengineering8120217.

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Extracted human teeth provide the closest approximation to teeth in situ and play important roles in dental education and materials research. Since extracted teeth are potentially infectious, the Centers for Disease Control recommend their sterilization by autoclaving or disinfection by formalin immersion to ensure safe handling. However, autoclaving is not recommended for teeth with amalgam fillings and formalin is hazardous. The goal of the present study was to investigate the potential of peracetic acid (PA) as an alternative method to achieve reliable disinfection of freshly extracted teet

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K, Pal A., Bhanarkar U. P, and Ray B. "Embalming with Formalin – Benefits and Pitfalls." Scholars International Journal of Anatomy and Physiology 5, no. 3 (2022): 70–77. http://dx.doi.org/10.36348/sijap.2022.v05i03.004.

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Formalin is the most widely used preservative in anatomical embalming. It produces consistent results in terms of the prevention of autolysis and putrefaction at an affordable cost. It is a known carcinogen having several health hazards. Strict adherence to safety protocols is required to minimize its toxic effects. Regular monitoring of formaldehyde concentration in the dissection hall and embalming room will ensure remedial measures at the earliest. In the face of growing health concerns, several alternative preservatives are being studied. Apart from it, methods like cryopreservation, plast

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Anggraini, Citra Dewi, S. Susiana, Putri Yurida Sari, Randi Dwi Anggriawan, and Muhamad Firdaus. "PERFORMA (PAPER TEST KIT FORMALIN) AS THE ALTERNATIVE SELECTION TO IMPROVE THE QUALITY OF FOOD INGREDIENTS." KnE Life Sciences 2, no. 1 (2015): 168. http://dx.doi.org/10.18502/kls.v1i0.101.

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Research on "PERFORMA" or Paper Test Kits Formalin is as an alternative to improve the quality of food selection. It is aiming to get a paper test kit that can detect formaldehyde contented in foodstuffs practically, fast, easy, and inexpensive. The method used in this study is an experimental method which is part of a quantitative method that aims to manipulate and control variables. The data collection is based on the research and the observation of the color change on the paper test kit is generated from Schiff reagent test, in which the paper test kit will give a purple color response when

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Parija, S. C., S. Bhattacharya, P. Padhan, and M. R. Shivaprakash. "Evaluation of Formalin — Acetone Sedimentation in the Concentration of Stool for Intestinal Parasites." Tropical Doctor 33, no. 3 (2003): 163–64. http://dx.doi.org/10.1177/004947550303300315.

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Formalin-acetone sedimentation was compared with the formalin-ether method for the concentration of stool for intestinal parasites. Of 80 stool specimens, 45 (56.25%) were positive for parasites by the formalin-acetone method. The figures for the two methods were formalin-ether 35 (43.75%) and for the direct lacto-phenol cotton blue wet mount method 17 (21.25%). There was no statistically significant difference in the parasite recovery rate between the two methods. Acetone is more stable, safer, and a cheaper fat solvent and promises to be a useful alternative to ether.

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Klopfleisch, R., M. von Deetzen, A. Th Weiss, et al. "Weigners Fixative–An Alternative to Formalin Fixation for Histology With Improved Preservation of Nucleic Acids." Veterinary Pathology 50, no. 1 (2012): 191–99. http://dx.doi.org/10.1177/0300985812441031.

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Formalin fixation and paraffin embedding (FFPE) is the standard method for tissue storage in histopathology. However, FFPE has disadvantages in terms of user health, environment, and nucleic acid integrity. Weigners fixative has been suggested as an alternative for embalming cadavers in human and veterinary anatomy. The present study tested the applicability of Weigners for histology and immunohistochemistry and the preservation of nucleic acids. To this end, a set of organs was fixed for 2 days and up to 6 months in Weigners (WFPE) or formalin. WFPE tissues from the skin, brain, lymphatic tis

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Ghazwan, Muhammad I. "Replacing Formalin with Alcohol and its Effect on Preserved Fish Specimens." IOP Conference Series: Earth and Environmental Science 1259, no. 1 (2023): 012085. http://dx.doi.org/10.1088/1755-1315/1259/1/012085.

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Abstract This study aims to formulate an alternative solution for Formalin for preserving fish as study specimens for long periods. The main reason for finding a solution instead of formalin is to get rid of the negative effects of this solution on those who work with it, as well as to better preserve the bodies of fish. Hence, three new solutions were proposed to replace formalin. Thus, Formalin, in turn, may enter the composition of a small part of these solutions to give better results and for long periods of keeping specimens. All solutions prepared in this study participated in being acid

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Mbiantcha, M., A. Kamanyi, R. B. Teponno, A. L. Tapondjou, P. Watcho, and T. B. Nguelefack. "Analgesic and Anti-Inflammatory Properties of Extracts from the Bulbils ofDioscorea bulbiferaL. varsativa(Dioscoreaceae) in Mice and Rats." Evidence-Based Complementary and Alternative Medicine 2011 (2011): 1–9. http://dx.doi.org/10.1155/2011/912935.

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The aqueous and methanol extracts from the dry bulbils ofDioscorea bulbiferaL. var sativa (Dioscoreaceae)—evaluated orally at the doses of 300 and 600 mg/kg against pain induced by acetic acid, formalin, pressure and against inflammation induced by carrageenan, histamine, serotonin and formalin in mice and rats, showed a dose dependant inhibition of pain and inflammation with a maximum effect of 56.38%, 73.06% and 42.79% produced by the aqueous extract, respectively on pain induced by acetic acid, formalin and pressure while the methanol extract at the same dose respectively inhibited these mo

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Zuo, Chuan-Yi, Peng Lv, Cheng-Shun Zhang, et al. "Ipsi- and Contralateral Moxibustion Generate Similar Analgesic Effect on Inflammatory Pain." Evidence-Based Complementary and Alternative Medicine 2019 (February 5, 2019): 1–7. http://dx.doi.org/10.1155/2019/1807287.

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The aim of this study was to investigate whether contralateral moxibustion would generate a similar analgesic effect with ipsilateral moxibustion. Contra- and ipsilateral moxibustion were separately applied to Zusanli (ST36) acupoints of inflammatory pain mice. The analgesic effect was evaluated, respectively, by licking/biting time (LBT) of formalin-induced inflammatory pain and thermal withdrawal latency (TWL) of complete Freund’s adjuvant- (CFA-) induced inflammatory pain. For formalin-induced pain, compared with formalin group, the total LBT of ipsi- and contralateral moxibustion reduced i

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Benerini Gatta, L., M. Cadei, P. Balzarini, et al. "Application of alternative fixatives to formalin in diagnostic pathology." European Journal of Histochemistry 56, no. 2 (2012): 12. http://dx.doi.org/10.4081/ejh.2012.12.

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Rosyidah, Afifah, Endang Purwanti, Djoko Hartanto, Irmina Kris Murwani, Didik Prasetyoko, and Ratna Ediati. "PENATAAN PKL BEBAS BORAKS DAN FORMALIN MENUJU PRODUK UNGGULAN SEHAT DAN HIGIENIS." QARDHUL HASAN: MEDIA PENGABDIAN KEPADA MASYARAKAT 3, no. 2 (2017): 86. http://dx.doi.org/10.30997/qh.v3i2.944.

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PKL STEPULATION OF FREE STORE BORAKS AND FORMALIN TOWARDS HEALTHY AND HYGIENIC SUPERIOR PRODUCTS ABSTRACT The Community service is focused on getting healthy and hygienic food around the campus. In the early stages, the identification of food sold in street vendors around the campus, whether it contains borax and or formalin. If the results of identification are still found foods containing borax and or formalin, then counseling and guidance and given about alternative materials borax and or formalin, which is safe for health. 40 food samples were random taken from street vendors around the ca

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Buvaneswari V, Deepadevi G, Peter Samidoss A, Muthu Prabha MS, and Vinoth Kumar V. "Qualitative analysis of histochemical and immunohistochemical properties of ethanol, glycerol, and formalin fixative." Asian Journal of Medical Sciences 16, no. 6 (2025): 122–29. https://doi.org/10.71152/ajms.v16i6.4515.

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Background: Fixation is essential in histopathology to preserve tissue integrity before analysis, with neutral buffered formalin being the gold standard for decades because of its effective protein cross-linking properties. Aims and Objectives: This study aimed to evaluate the histochemical and immunohistochemical properties of ethanol, glycerol, and formalin (EGF) fixative and to compare and analyze the impact of fixation using conventional formalin and EGF fixative on special histochemical stains and immunohistochemical studies. Materials and Methods: A minimal formalin containing EGF fixati

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Kim, Jae Hyo, Young Seob Gwak, Inhyung Lee, et al. "Antinociceptive Effects of Heterotopic Electroacupuncture in Formalin-Induced Pain." American Journal of Chinese Medicine 34, no. 04 (2006): 565–74. http://dx.doi.org/10.1142/s0192415x06004107.

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This study examined the antinociceptive effect of electroacupuncture (EA) to heterotopic acupoints on formalin-induced pain in rats. EA (2 ms, 10 Hz, and 3 mA) was delivered to heterotopic acupoints HE7 and PE7, or non-acupoints at the right fore limb, for 30 min and was immediately followed by subcutaneous formalin injection into the left hind paw, respectively. The quantified pain score, electromyogram (EMG) response of the C-fiber reflex, and cFos immunoreactivity were assessed, respectively. EA to heterotopic acupoints significantly reduced both early- and late-phase pain-like behaviors an

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Gullaiya, Jyoti, Naresh Kumar, and Neeharika Srivastava. "Effect of formalin fixation on DNA: A Time-Based Approach." Indian Journal of Forensic Medicine and Pathology 14, no. 4 (2021): 825–30. http://dx.doi.org/10.21088/ijfmp.0974.3383.14421.7.

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Introduction:Tissue preservation is important. From it forensic scientist may extract sufficient DNA for profiling. The most commonly used preservative found in the literature is formalin. However, it causes severe side-effects on its users as well as the environment; we need to find its replacement. Method:This study examined whether formalin could preserve soft tissues (fresh) stored at 4°C for 100 days and how DNA can be extracted from it. Qualification and Quantification of the presevred samples were done. Result:The results revealed that tissues preserved in formalin failed to generate su

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Chauhan, K., A. Sharma, B. K. Mohapatra, R. Kaushik, P. Shukla, and C. Behera. "DNA extraction from formalin fixed tissue using an alternative protocol." Journal of Forensic Medicine and Toxicology 38, no. 2 (2021): 78–80. http://dx.doi.org/10.5958/0974-4568.2021.00039.9.

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Pernak, Agnieszka, Katarzyna Iwanik, Przemysław Majewski, Marian Grzymisławski, and Juliusz Pernak. "Ionic liquids as an alternative to formalin in histopathological diagnosis." Acta Histochemica 107, no. 2 (2005): 149–56. http://dx.doi.org/10.1016/j.acthis.2005.02.003.

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Lee, Seung Jae, Yeoung Su Lyu, Hyung Won Kang, et al. "Antinociception of Heterotopic Electro-Acupuncture Mediated by the Dorsolateral Funiculus." American Journal of Chinese Medicine 35, no. 02 (2007): 251–64. http://dx.doi.org/10.1142/s0192415x07004795.

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We investigated the inhibitory pathways that mediate the antinociceptive effects of heterotopic electro-acupuncture (EA) on formalin injection-induced pain in rats. EA (2 ms, 10 Hz, 3 mA) was delivered to heterotopic acupoints HT7 and PC7 for 30 min; this was followed immediately by subcutaneous injection of formalin into the left hind paw of rats. Naltrexone (10 mg/kg, i.p.), an opioid receptor antagonist, was administered to evaluate the involvement of endogenous opioids. The dorsolateral funiculus (DLF), which is a descending pathway that inhibits pain, was transected at the ipsilateral T10

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Chiwar, Hassan, A. T. Alkali, A. I. Musa, and H. B. Umar. "EXPLORING THE POTENTIAL OF MORINGA OLEIFERA EXTRACT AS A NATURAL TISSUE FIXATIVE FOR HISTOPATHOLOGICAL APPLICATIONS." FUDMA JOURNAL OF SCIENCES 8, no. 1 (2024): 238–49. http://dx.doi.org/10.33003/fjs-2024-0801-2267.

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Histopathology, crucial for disease diagnosis, heavily relies on effective tissue fixation to maintain cellular architecture. However, the carcinogenic nature of formalin has spurred interest in safer alternatives. Moringa oleifera, renowned for its medicinal properties, presents a promising natural fixative candidate. This study aimed to assess Moringa oleifera extract's viability as a fixative for histopathological applications, specifically focusing on its compatibility with subsequent histological analyses. Distilled water and normal saline solutions of Moringa extract were prepared at var

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Bugshan, Amr S., Hawra A. AlJanobi, Raghad A. AlMunif, et al. "Propolis as a Potential Novel Histological Tissue Fixative: A Preliminary Analysis." Applied Sciences 12, no. 19 (2022): 9842. http://dx.doi.org/10.3390/app12199842.

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Background: Fixation of biopsy specimens is a critical step before processing and staining them for histological tissue examination. Formalin is considered the gold standard fixative solution for its attributes. However, it has concerning side effects, such as carcinogenic and potential irritational properties. Owing to its various harmful effects, a safer natural substitute should be explored. In this study, we compared the effectiveness of propolis to 10% formalin and determined its ability as a natural fixative solution. Materials and Methods: Sixty tissue specimens were collected from goat

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Aboc, Tricia S., Micah Angela A. Lopez, and Ericka L. Sabus. "The effectiveness of formalin from sweet orange (Citrus sinensis) peel extract in histopathologic tissue preparation as compared to commercially prepared formalin." SDCA Student Research Journal 11, no. 1 (2022): 49–54. https://doi.org/10.5281/zenodo.14272278.

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This research was centered to compare the formaldehyde content of the sweet orange peel extract to commercially prepared 10% neutral buffered formalin. The chosen two (2) specimens were pig’s heart and liver. As replacement to human tissue, the pig provides specimens closely resembling human tissue. In spite of these advantages in Formalin, the health and safety risks are of concern and the International Agency for Research on Cancer (IARC) emphasized it as Group-A carcinogen. Motivated by this, the researchers made this research to find safer alternatives. The sweet orange peel was grat

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Thamilselvan, Snega, Don K. R, Herald J. Sherlin, Gifrina Jayaraj, and Archana Santhanam. "Honey as a Natural Alternative for Formalin Fixative - A Systematic Review." Journal of Evolution of Medical and Dental Sciences 10, no. 6 (2021): 369–74. http://dx.doi.org/10.14260/jemds/2021/82.

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Andrews, B. A. Kottes. "1,3,-Dioxolane, An Alternative to Formalin as a Standard for Formaldehyde." Textile Research Journal 57, no. 12 (1987): 705–10. http://dx.doi.org/10.1177/004051758705701205.

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Saowakon, Naruwan, Apichart Ngernsoungnern, Piyada Ngernsoungnern, Chompunoot Wangboon, and Oratai Weeranantanapan. "Comparison of modified Thiel formalin-free embalming with traditional Thiel and formalin techniques in a rabbit model for soft cadaver preservation." European Journal of Anatomy 29, no. 4 (2025): 585–97. https://doi.org/10.52083/iyxl4743.

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Cadavers are essential tools for teaching gross anatomy to medical and health science students. Traditional formalin-embalmed cadavers preserve anatomical structures but exhibit rigidity and limited joint mobility, making dissection difficult. While the Thiel embalming method preserves lifelike properties, the presence of formalin/formaldehyde raises occupational health concerns. This study evaluates a modified formalin-free Thiel solution (FF) compared to the original Thiel solution (TS) and conventional formalin solution (CF). Nine rabbits were allocated to three groups and preserved for one

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Barcellos, Jiames Nunes, Márcia da Silva Schmitz, Michele Rorato Sagrillo, and Liana da Silva Fernandes. "Production, characterization and biological evaluation of nanocapsules containing tricresol formalin and their comparison with the free form." International Journal for Innovation Education and Research 9, no. 11 (2021): 218–45. http://dx.doi.org/10.31686/ijier.vol9.iss11.3556.

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Ethnopharmacological relevance: Tricresol formalin is composed of 90% formaldehyde and 10% cresols, highly volatile, has action at a distance, has been used in endodontics since the 20th century, and it remains widely used in Brazil in dental treatments, in necrotic teeth and with periapical lesions. However, there is still controversy regarding the biological compatibility under the conditions of clinical use of this drug, as the studies carried out on this substance and its components are not consistent with its clinical use. Formaldehyde is reported as a potential cytotoxic substance, becau

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IJ, Gosomji, Omirinde JO, Hena SA, Wanmi N, and Azeez IA. "Saturated salt solution an alternative reagent in reducing formaldehyde concentration in embalming." MOJ Anatomy & Physiology 5, no. 3 (2018): 205–7. http://dx.doi.org/10.15406/mojap.2018.05.00192.

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A well embalmed cadaver for a participatory dissection should be able to have long-term structural preservation of organs and tissues with minimal distortion, deterred from hardening or any form of modification. Six (6) goats meant for students’ dissection practical were utilized for this study in the period of six (6) months. They were grouped into A, B and C based on concentration of formaldehyde in relation to saturated salt solution. Stock solution of saturated salt solution (SSS) was used to make 5% and 10% of formalin used in grouped A and B respectively while group C was 20% formalin wi

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Puhar, Jan, Annamaria Vujanović, Peter Awad, and Lidija Čuček. "Reduction of Cost, Energy and Emissions of the Formalin Production Process via Methane Steam Reforming." Systems 9, no. 1 (2021): 5. http://dx.doi.org/10.3390/systems9010005.

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Production of formalin, which is among the highest production volume chemicals, is highly energy-intensive; thus, reduction of energy use is very important in reducing cost and emissions. The aim of this and its larger overall research is to systemically analyze how to improve sustainability of processes producing formalin as an intermediate or final product. In this part of the work, energy consumption requirements are analyzed for the conventional formalin production process via methane steam reforming, where opportunities for energy consumption reduction are identified. This work will serve

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Yamamoto, Shutaro, Yoshitaka Kinoshita, Haruki Kume, Takahiro Kimura, Takashi Yokoo, and Eiji Kobayashi. "Proposal of a Safe Transport Protocol and Its Utility of Antigen-Preserving Tissue for Formalin-Fixed Porcine Renal Samples." Biomedicines 13, no. 4 (2025): 831. https://doi.org/10.3390/biomedicines13040831.

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Background: Formalin is widely used as a standard fixative in histopathological analysis; however, its high toxicity and strict regulatory restrictions create challenges for the safe transport and external evaluation of specimens. In translational research utilizing large animal models, establishing a reliable transport protocol that preserves both tissue structure and antigenicity remains essential. Objective: This study aimed to develop and validate a protocol for the safe transport of formalin-fixed renal specimens while maintaining their histopathological and immunohistochemical integrity.

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Magalhães, Francisco Ernani Alves, Caio Átila Prata Bezerra de Sousa, Sacha Aubrey Alves Rodrigues Santos, et al. "Adult Zebrafish (Danio rerio): An Alternative Behavioral Model of Formalin-Induced Nociception." Zebrafish 14, no. 5 (2017): 422–29. http://dx.doi.org/10.1089/zeb.2017.1436.

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Southwood, M., and D. Rassl. "45 “PAXgene Tissue” – a novel alternative to formalin fixation for diagnostic pathology." Lung Cancer 91 (January 2016): S16. http://dx.doi.org/10.1016/s0169-5002(16)30062-9.

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Kacena, MA, NW Troiano, CE Coady, and MC Horowitz. "HistoChoice® as an alternative to formalin fixation of undecalcified bone specimens." Biotechnic & Histochemistry 79, no. 5-6 (2004): 185–90. http://dx.doi.org/10.1080/10520290400015506.

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RAHMAN, AVE SONIA, TJAHJADI PURWOKO, and ESTU RETNANINGTYAS NUGRAHENI. "The influence of ethanol extracts concentration of mengkudu fruit (Morinda citrifolia) and storage time toward beef quality." Biofarmasi Journal of Natural Product Biochemistry 9, no. 1 (2011): 26–32. http://dx.doi.org/10.13057/biofar/f090105.

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Rahman AS, Purwoko T, Nugraheni ER. 2011. The influence of ethanol extracts concentration of mengkudu fruit (Morinda citrifolia) and storage time toward beef quality. Biofarmasi 9: 26-32. The high protein content of beef makes it easy to get a contamination caused by an activity of bacteria. The use of formalin as a preservative substance especially for beef can make a damage to health, so it is required to find preservative substances as alternative to substitute formalin. Mengkudu (Morinda citrifolia L.) has antibacteria compounds to the decompose bacteria of beef, so mengkudu fruit can be o

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Taha, Safa, Amina Ismaeel, Muna Aljishi, Samvel Selvam, Angeleena Esther, and Khaled Greish. "Silver Nanoparticles as a Novel Tissue Preservative: A Comparative Study with 10% Neutral Buffered Formalin." International Journal of Molecular Sciences 26, no. 11 (2025): 5335. https://doi.org/10.3390/ijms26115335.

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Tissue preservation plays an essential role in biomedical research and histopathological applications. Traditional methods, despite their efficiency, are associated with compromised long-term tissue integrity and probable ecotoxicities. This study explores the application of silver nanoparticles (AgNPs), known for their antimicrobial properties, as a potential tissue preservative. In this work, AgNPs were synthesized via a chemical reduction method. Heart, liver, and kidney tissues were obtained from BALB/c mice and preserved using 10% neutral buffered formalin (NBF) and AgNPs solution for 72

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Nawaz, Shabana, AH Nagi, Ayesha Bajwa, Aneela Amjad, Hafiz Mohammad Dawood, and Maham Ashraf. "Comparison of Honey and Alcoholic Fixatives in Tissues Using Different Types of Stains." Healer Journal of Physiotherapy and Rehabilitation Sciences 3, no. 8 (2023): 776–84. https://doi.org/10.55735/hjprs.v3i8.174.

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Background: Formalin has been used as a standard fixative for various clinical routines globally. In 1987, after formaldehyde was used as a standard, various studies have been conducted to find a substitute for formalin due to its carcinogenic effects. Since then honey has been proven to be a safe alternative to formalin. Objective: Comparing the tissue fixation abilities of honey, Carnoy’s, Bouin’s and Zenker’s solution with the tissue fixation abilities of formalin using eosin, hematoxylin and Masson trichrome periodic acid Schiff is the objective of this study. Methods: This current study i

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Ribeiro, Joice Gabriele Rocha, Alana Ferraz de Melo Santos, Aléxia David Santos Soares, et al. "Glycerination as an alternative technique for preserving Vertebrata (Osteichthyes and Reptilia): a proposal for teaching purposes." Acta Scientiarum. Biological Sciences 46 (December 5, 2024): e71746. https://doi.org/10.4025/actascibiolsci.v46i1.71746.

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Biological specimens are essential for teaching, research, and extension, although they require effective preservation. The glycerination technique stands out for allowing specimens to be preserved dry, which saves costs and facilitates handling due to specimen malleability. We sought to establish an optimal glycerination protocol for fish and reptiles for teaching purposes. Several fixatives were tested, including formalin at different concentrations and Transeau solution. The fixation process involved injecting a 50% fixative and 50% double-distilled glycerin solution into the specimen, foll

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Khoirunnida, Avivi, Tri Wiharti, and Ratna Dewi Eskundari. "Test for Formalin Content in Consumed Fish at Several Traditional Markets in Surakarta Using Natural Extract of Dragon Fruit Peel." Jurnal Biologi Tropis 23, no. 3 (2023): 343–51. http://dx.doi.org/10.29303/jbt.v23i3.5177.

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Food has an expiration date. To make food have a longer shelf life, food manufacturers use preservatives as a solution. There are preservatives that are safe to use and prohibited from being used. One of the chemical preservatives prohibited from being used in food is formalin. Formalin is usually added to processed meat and fish foods. The purpose of this study was to detect the presence of formaldehyde as a preservative in consumption fish sold in several markets in Surakarta. The method used in this study is experimental. As an indicator in this study, natural ingredients were used, namely

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Bribi, Noureddine, Francesca Algieri, Alba Rodriguez-Nogales, et al. "Antinociceptive and Anti-Inflammatory Effects of Total Alkaloid Extract fromFumaria capreolata." Evidence-Based Complementary and Alternative Medicine 2015 (2015): 1–7. http://dx.doi.org/10.1155/2015/736895.

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Fumaria capreolatais used in traditional medicine in North Africa for its gastrointestinal and anti-inflammatory activities. The present study investigates the effects of total alkaloids extracted from the aerial parts ofFumaria capreolata(AFC) on LPS-induced production of proinflammatory mediators (IL-6, IL-1β, iNOS, TNF-α, COX-2, and MIP-2) in RAW264.7 cells. AFC significantly reduced the inflammatory response inhibiting the production of nitric oxide (NO) and IL-6 in a dose-dependent manner, without affecting the viability of cells, and downregulated mRNA expression of proinflammatory key p

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Shetty, Urvashi A., Chethana Dinakar, Audrey M. D’Cruz, Pushparaja Shetty, and Vishnudas Prabhu. "Plastination - A method for preservation of oral hard and soft tissue biopsy specimen v/s the conventional method of preservation with formalin." Journal of Oral and Maxillofacial Pathology 27, no. 3 (2023): 515–19. http://dx.doi.org/10.4103/jomfp.jomfp_132_23.

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Context: Plastination is one of the most advanced method for preserving perishable biological specimen as well as tissue samples as for a longer period of time using polymers. Aims: To evaluate the changes in dimension of hard and soft tissue specimens after plastination procedure and compare it with the conventional method of preservation in formalin. Settings and Design: Institution-based retrospective study. Methods and Material: The study included 20 formalin-fixed soft tissue and 10 formalin-fixed hard tissue specimens. All the specimens were plastinated which involved four basic steps of

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Interesova, Elena Aleksandrovna, Alexander Alekseevich Rostovtsev, and Alexey Sergeevich Knyazev. "Prospects for using the preparation “Aldofix” as a substitute for formalin in studies of juvenile fish." Rybovodstvo i rybnoe hozjajstvo (Fish Breeding and Fisheries), no. 11 (November 1, 2020): 74–81. http://dx.doi.org/10.33920/sel-09-2011-07.

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The study of juvenile fish is a necessary component of fishery research, is of great theoretical interest and is widely used in the study of ontogeny, influence of various environmental factors on the final trophic link of aquatic ecosystems. Traditionally, fry samples are fixed with a 4–5 % formalin solution. However, formalin is a toxic compound, which entails special requirements for the organization of work in laboratory. In order to search for alternative means of fixing biological preparations, an experimental assessment of the possibility of using the drug “Aldofix” as a substitute for

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Choi, Seong-Soo, Eun-Jung Han, Tae-Hee Lee, Ki-Jung Han, Han-Kyu Lee, and Hong-Won Suh. "Antinociceptive Profiles of Platycodin D in the Mouse." American Journal of Chinese Medicine 32, no. 02 (2004): 257–68. http://dx.doi.org/10.1142/s0192415x04001916.

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Platycodin D (PD), one of several triterpene saponins, was isolated from roots of Platycodon grandiflorum. We previously reported that intracerebroventricular (i.c.v.) administration of PD showed an antinociceptive effect as measured by the tail-flick assay. However, its exact role in the regulation of antinociception in the various types of pain models has not yet been characterized. Thus, we attempted to find antinociceptive profiles of PD in various pain models. PD administered intraperitoneally (i.p.), i.c.v. or intrathecally (i.t.) showed antinociceptive effects in dose-dependent manners

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Liao, Chi-Ren, Chun-Pin Kao, Wen-Huang Peng, Yuan-Shiun Chang, Shang-Chih Lai, and Yu-Ling Ho. "Analgesic and Anti-Inflammatory Activities of Methanol Extract ofFicus pumilaL. in Mice." Evidence-Based Complementary and Alternative Medicine 2012 (2012): 1–9. http://dx.doi.org/10.1155/2012/340141.

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This study investigated possible analgesic and anti-inflammatory mechanisms of the methanol extract ofFicus pumila(FPMeOH). Analgesic effects were evaluated in two models including acetic acid-induced writhing response and formalin-induced paw licking. The results showedFPMeOHdecreased writhing response in the acetic acid assay and licking time in the formalin test. The anti-inflammatory effect was evaluated by λ-carrageenan-induced mouse paw edema and histopathological analyses.FPMeOHsignificantly decreased the volume of paw edema induced by λ-carrageenan. Histopathologically,FPMeOHabated the

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AARON GALEA, Laurence, Sue STURROCK, Sree APPU, Daniel LANTSBERG, and Darren KATZ. "Improving Interpretation of Biopsies During Microsurgical Testicular Sperm Exploration in Azoospermic Patients: Use of Davidson’s Fluid." Fertility & Reproduction 04, no. 03n04 (2022): 192. http://dx.doi.org/10.1142/s2661318222741030.

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Background: Histological interpretation of testicular biopsies in the investigation of infertility in men with azoospermia requires adequate tissue fixation to preserve the nuclear and cytoplasmic detail, as well as the architectural organisation of germ cells in different phases of maturation within seminiferous tubules. Research Aims/Objectives: The aim of the study was to assess the histomorphological quality of testicular biopsies using Davidson’s fluid (DF) as fixative and compare it to standard 10% neutral buffered Formalin. Methodology: Concurrent testicular biopsies from the same testi

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Hewitt, Stephen M., Fraser A. Lewis, Yanxiang Cao, et al. "Tissue Handling and Specimen Preparation in Surgical Pathology: Issues Concerning the Recovery of Nucleic Acids From Formalin-Fixed, Paraffin-Embedded Tissue." Archives of Pathology & Laboratory Medicine 132, no. 12 (2008): 1929–35. http://dx.doi.org/10.5858/132.12.1929.

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Abstract Context.—Expression profiling by microarrays and real-time polymerase chain reaction–based assays is a powerful tool for classification and prognostication of disease; however, it remains a research tool, largely reliant on frozen tissue. Limiting the utility of expression profiling is the isolation of quality nucleic acids from formalin-fixed, paraffin-embedded tissue. The collection, handling, and processing of tissue directly impacts the biomolecules that can be recovered from it. High-quality nucleic acids can be obtained from formalin-fixed, paraffin-embedded tissue, but greater

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Nabil, Nabil. "PERBEDAAN HISTOPATOLOGI ANATOMI HEPAR KELINCI SETELAH 24 JAM KEMATIAN MENGGUNAKAN LARUTAN METANOL + TANNIN DAN LARUTAN FORMALIN SEBAGAI BAHAN PENGAWETAN." E-Jurnal SPIRIT PRO PATRIA 7, no. 2 (2021): 77–83. http://dx.doi.org/10.29138/spirit.v7i2.1867.

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Latar belakang Sebagai praktisi dalam pengawetan terhadap jenazah, kesulitan pengadaan larutan formalin dan efek samping paparan terhadap kesehatan manusia merupakan suatu permasalahan serius yang menjadi perhatian khusus dari peneliti, untuk itu peneliti mencoba memakai larutan Methanol ditambah Tannin sebagai alternative yang aman bagi kesehatan dan mudah di dalam pengadaannya. Tujuan penelitian untuk mengetahui perbedaan penggunaan campuran larutan metanol dan Tannin dibandingkan dengan penggunaan larutan formalin sebagai bahan pengawetan kelinci putih jantan (oryctolagus cuniculus) jenis r

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Al-Maaini, Rahma, and Philip Bryant. "Honey as an Alternative to Formalin in the Demonstration of Connective Tissue Components." Journal of Histotechnology 31, no. 2 (2008): 67–72. http://dx.doi.org/10.1179/his.2008.31.2.67.

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Bussolati, Gianni, Luigi Chiusa, Antonio Cimino, and Giuseppe D’Armento. "Tissue transfer to pathology labs: under vacuum is the safe alternative to formalin." Virchows Archiv 452, no. 2 (2007): 229–31. http://dx.doi.org/10.1007/s00428-007-0529-x.

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