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Journal articles on the topic 'Formes recombinées'

Author: Grafiati

Published: 4 June 2021

Last updated: 16 February 2022

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1

SITHARAM, MEERA. "WELL-FORMED SYSTEMS OF POINT INCIDENCES FOR RESOLVING COLLECTIONS OF RIGID BODIES." International Journal of Computational Geometry & Applications 16, no. 05n06 (2006): 591–615. http://dx.doi.org/10.1142/s0218195906002208.

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For tractability, many modern geometric constraint solvers recursively decompose an input geometric constraint system into standard collections of smaller, generically rigid subsystems or clusters. These are recursively solved and their solutions or realizations are recombined to give the solution or realization of the input constraint system. Even for generically wellconstrained systems in 3D, and even when the shared objects between clusters in the decomposition are restricted to be points, it is a significant hurdle to find a wellformed system of shared object incidences that recombines a c

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2

Goyon, C., and M. Lichten. "Timing of molecular events in meiosis in Saccharomyces cerevisiae: stable heteroduplex DNA is formed late in meiotic prophase." Molecular and Cellular Biology 13, no. 1 (1993): 373–82. http://dx.doi.org/10.1128/mcb.13.1.373.

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To better understand the means by which chromosomes pair and recombine during meiosis, we have determined the time of appearance of heteroduplex DNA relative to the times of appearance of double-strand DNA breaks and of mature recombined molecules. Site-specific double-strand breaks appeared early in meiosis and were formed and repaired with a timing consistent with a role for breaks as initiators of recombination. Heteroduplex-containing molecules appeared about 1 h after double-strand breaks and were followed shortly by crossover products and the first meiotic nuclear division. We conclude t

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3

Goyon, C., and M. Lichten. "Timing of molecular events in meiosis in Saccharomyces cerevisiae: stable heteroduplex DNA is formed late in meiotic prophase." Molecular and Cellular Biology 13, no. 1 (1993): 373–82. http://dx.doi.org/10.1128/mcb.13.1.373-382.1993.

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To better understand the means by which chromosomes pair and recombine during meiosis, we have determined the time of appearance of heteroduplex DNA relative to the times of appearance of double-strand DNA breaks and of mature recombined molecules. Site-specific double-strand breaks appeared early in meiosis and were formed and repaired with a timing consistent with a role for breaks as initiators of recombination. Heteroduplex-containing molecules appeared about 1 h after double-strand breaks and were followed shortly by crossover products and the first meiotic nuclear division. We conclude t

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4

Sauvageau, Synthia, Alicja Z. Stasiak, Isabelle Banville, Mickaël Ploquin, Andrzej Stasiak, and Jean-Yves Masson. "Fission Yeast Rad51 and Dmc1, Two Efficient DNA Recombinases Forming Helical Nucleoprotein Filaments." Molecular and Cellular Biology 25, no. 11 (2005): 4377–87. http://dx.doi.org/10.1128/mcb.25.11.4377-4387.2005.

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ABSTRACT Homologous recombination is important for the repair of double-strand breaks during meiosis. Eukaryotic cells require two homologs of Escherichia coli RecA protein, Rad51 and Dmc1, for meiotic recombination. To date, it is not clear, at the biochemical level, why two homologs of RecA are necessary during meiosis. To gain insight into this, we purified Schizosaccharomyces pombe Rad51 and Dmc1 to homogeneity. Purified Rad51 and Dmc1 form homo-oligomers, bind single-stranded DNA preferentially, and exhibit DNA-stimulated ATPase activity. Both Rad51 and Dmc1 promote the renaturation of co

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5

Nastasi, Francesco, Fausto Puntoriero, Mirco Natali, et al. "Photoinduced intercomponent excited-state decays in a molecular dyad made of a dinuclear rhenium(i) chromophore and a fullerene electron acceptor unit." Photochemical & Photobiological Sciences 14, no. 5 (2015): 909–18. http://dx.doi.org/10.1039/c4pp00301b.

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6

Sorokin, V. V. "Аnalysis Catalytic Hydrogen Recombiner Capacity Calculation Taking into Account Conditions Inside Sealed Enclosure of Containment Safety System of Nuclear Power Plants with Water-Water Energetic Reactor". ENERGETIKA. Proceedings of CIS higher education institutions and power engineering associations 64, № 2 (2021): 178–86. http://dx.doi.org/10.21122/1029-7448-2021-64-2-178-186.

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Localizing safety systems are provided to contain radioactive substances in an accident and attenuate ionizing radiation at a modern nuclear power plant. Together with radioactive substances, hydrogen is also retained, which is formed during the decomposition of the primary coolant. The accumulation of hydrogen in the presence of oxygen from the atmosphere in the accident localization zone carries the danger of the formation of flammable and explosive concentrations of these components. Nuclear power plant (NPP) deigns with water-water energetic reactor (WWER) provides for a hydrogen removal s

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7

Manil-Ségalen, Marion, Małgorzata Łuksza, Joanne Kanaan, et al. "Chromosome structural anomalies due to aberrant spindle forces exerted at gene editing sites in meiosis." Journal of Cell Biology 217, no. 10 (2018): 3416–30. http://dx.doi.org/10.1083/jcb.201806072.

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Mouse female meiotic spindles assemble from acentriolar microtubule-organizing centers (aMTOCs) that fragment into discrete foci. These are further sorted and clustered to form spindle poles, thus providing balanced forces for faithful chromosome segregation. To assess the impact of aMTOC biogenesis on spindle assembly, we genetically induced their precocious fragmentation in mouse oocytes using conditional overexpression of Plk4, a master microtubule-organizing center regulator. Excessive microtubule nucleation from these fragmented aMTOCs accelerated spindle assembly dynamics. Prematurely fo

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8

Bailey, S. N., and N. Rosenberg. "Assessing the pathogenic potential of the V(D)J recombinase by interlocus immunoglobulin light-chain gene rearrangement." Molecular and Cellular Biology 17, no. 2 (1997): 887–94. http://dx.doi.org/10.1128/mcb.17.2.887.

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Chromosomal translocations involving antigen receptor genes and oncogenes have been observed in several forms of lymphoid malignancy. Observations of their lymphocyte-restricted occurrence and a molecular analysis of some translocation breakpoints have suggested that some of these rearrangements are generated by V(D)J recombinase activity. However, a direct correlation between this activity and the generation of such rearrangements has never been established. In addition, because these aberrant rearrangements are usually detected only after a tumor has been formed, the frequency with which the

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9

Sciochetti, Stephen A., Patrick J. Piggot, and Garry W. Blakely. "Identification and Characterization of thedif Site from Bacillus subtilis." Journal of Bacteriology 183, no. 3 (2001): 1058–68. http://dx.doi.org/10.1128/jb.183.3.1058-1068.2001.

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ABSTRACT Bacteria with circular chromosomes have evolved systems that ensure multimeric chromosomes, formed by homologous recombination between sister chromosomes during DNA replication, are resolved to monomers prior to cell division. The chromosome dimer resolution process inEscherichia coli is mediated by two tyrosine family site-specific recombinases, XerC and XerD, and requires septal localization of the division protein FtsK. The Xer recombinases act near the terminus of chromosome replication at a site known asdif (Ecdif). In Bacillus subtilisthe RipX and CodV site-specific recombinases

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10

Hayashi, K., S. Yasugi, and T. Mizuno. "Pepsinogen gene transcription induced in heterologous epithelial-mesenchymal recombinations of chicken endoderms and glandular stomach mesenchyme." Development 103, no. 4 (1988): 725–31. http://dx.doi.org/10.1242/dev.103.4.725.

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Proventricular (glandular stomach) mesenchyme of chicken embryos can induce endoderms of some parts of embryonic digestive tract to produce embryonic chicken pepsinogen (ECPg), a marker protein for the differentiation of embryonic proventricular epithelium. In the present study, we investigated the production of ECPg mRNA in the course of epithelial-mesenchymal interactions between endoderms of digestive tract and proventricular mesenchyme. ECPg mRNA was detected by Northern hybridization with ECPg cDNA as a probe. In normal development of the proventriculus, ECPg mRNA was first detected at da

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11

Suzuki, Masaaki, Saburo Neya, and Yutaka Nishigaichi. "Unusual C–C bond formation between recombined N-fused pentaphyrin and amine nucleophiles." Journal of Porphyrins and Phthalocyanines 20, no. 06 (2016): 738–43. http://dx.doi.org/10.1142/s1088424616500632.

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Skeletally recombined [Formula: see text]-fused pentaphyrin bromide underwent aromatic nucleophilic substitution-like reactions when treated with several amine reagents. As a result, an irregular bond such as a vinylene or phenylene linkage was formed between the inside of the macrocycle and the amine nitrogen. This linkage extended the conjugation network and created a “switchback [Formula: see text]-plane” in the product, achieving a new kind of three-dimensional zigzag-like delocalization of [Formula: see text]-electrons.

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Stokes, Russell H., Jonathan S. Oakhill, Christopher L. Joannou, Andrew R. Gorringe, and Robert W. Evans. "Meningococcal Transferrin-Binding Proteins A and B Show Cooperation in Their Binding Kinetics for Human Transferrin." Infection and Immunity 73, no. 2 (2005): 944–52. http://dx.doi.org/10.1128/iai.73.2.944-952.2005.

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ABSTRACT Neisseria meningitidis, a causative agent of bacterial meningitis and septicemia, obtains transferrin-bound iron by expressing two outer membrane-located transferrin-binding proteins, TbpA and TbpB. A novel system was developed to investigate the interaction between Tbps and human transferrin. Copurified TbpA-TbpB, recombined TbpA-TbpB, and individual TbpA and TbpB were reconstituted into liposomes and fused onto an HPA chip (BIAcore). All preparations formed stable monolayers, which, with the exception of TbpB, could be regenerated by removing bound transferrin. The ligand binding pr

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13

Kong, Qingyu, Jae Hyuk Lee, Manuela Lo Russo, et al. "Photolysis of Br2in CCl4studied by time-resolved X-ray scattering." Acta Crystallographica Section A Foundations of Crystallography 66, no. 2 (2010): 252–60. http://dx.doi.org/10.1107/s0108767309054993.

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A time-resolved X-ray solution scattering study of bromine molecules in CCl4is presented as an example of how to track atomic motions in a simple chemical reaction. The structures of the photoproducts are tracked during the recombination process, geminate and non-geminate, from 100 ps to 10 µs after dissociation. The relaxation of hot Br2*molecules heats the solvent. At early times, from 0.1 to 10 ns, an adiabatic temperature rise is observed, which leads to a pressure gradient that forces the sample to expand. The expansion starts after about 10 ns with the laser beam sizes used here. When th

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14

Frahsek, Madeleine, Kevin Schulte, Arnaldo Chia-Gil, et al. "Cre recombinase toxicity in podocytes: a novel genetic model for FSGS in adolescent mice." American Journal of Physiology-Renal Physiology 317, no. 5 (2019): F1375—F1382. http://dx.doi.org/10.1152/ajprenal.00573.2018.

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Here, we show that inducible overexpression of Cre recombinase in glomerular podocytes but not in parietal epithelial cells may trigger focal segmental glomerulosclerosis (FSGS) in juvenile transgenic homocygous Pod-rtTA/LC1 mice. Administration of doxycycline shortly after birth, but not at any other time point later in life, resulted in podocyte injury and development of classical FSGS lesions in these mice. Sclerotic lesions were formed as soon as 3 wk of age, and FSGS progressed with low variability until 13 wk of age. In addition, our experiments identified Cre toxicity as a potentially r

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15

Marko, John F., and Michael G. Poirier. "Micromechanics of chromatin and chromosomes." Biochemistry and Cell Biology 81, no. 3 (2003): 209–20. http://dx.doi.org/10.1139/o03-047.

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The enzymes that transcribe, recombine, package, and duplicate the eukaryotic genome all are highly processive and capable of generating large forces. Understanding chromosome function therefore will require analysis of mechanics as well as biochemistry. Here we review development of new biophysical-biochemical techniques for studying the mechanical properties of isolated chromatin fibers and chromosomes. We also discuss microscopy-based experiments on cells that visualize chromosome structure and dynamics. Experiments on chromatin tell us about its flexibility and fluctuation, as well as quan

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16

Pezza, Roberto J., Oleg N. Voloshin, Alexander A. Volodin, et al. "The dual role of HOP2 in mammalian meiotic homologous recombination." Nucleic Acids Research 42, no. 4 (2013): 2346–57. http://dx.doi.org/10.1093/nar/gkt1234.

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Abstract Deletion of Hop2 in mice eliminates homologous chromosome synapsis and disrupts double-strand break (DSB) repair through homologous recombination. HOP2 in vitro shows two distinctive activities: when it is incorporated into a HOP2–MND1 complex it stimulates DMC1 and RAD51 recombination activities and the purified HOP2 alone is proficient in promoting strand invasion. We observed that a fraction of Mnd1−/− spermatocytes, which express HOP2 but apparently have inactive DMC1 and RAD51 due to lack of the HOP2–MND1 complex, exhibits a high level of chromosome synapsis and that most DSBs in

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17

Litter, Marta I., and Miguel A. Blesa. "Photodissolution of iron oxides II: The lack of efficiency of thiocyanate." Canadian Journal of Chemistry 68, no. 5 (1990): 728–30. http://dx.doi.org/10.1139/v90-114.

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Thiocyanate does not enhance, and even inhibits, the photochemical dissolution of iron oxides at 254 nm. At low SCN− concentrations, surface complexes [Formula: see text] are formed only to a small extent due to the low affinity for the surface by the monovalent anion, and electron-hole pairs essentially recombine. At higher ligand concentrations, only the homogeneous photolysis of SCN− is observed. At 450 nm, there is no appreciable light absorption by the oxide but homogeneous photolysis of FeIII—SCN− complexes produces FeII, which dissolves the oxide by a reductive mechanism. Keywords: iron

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18

Rosen, David A., Jerome S. Pinkner, Jennifer M. Jones, Jennifer N. Walker, Steven Clegg, and Scott J. Hultgren. "Utilization of an Intracellular Bacterial Community Pathway in Klebsiella pneumoniae Urinary Tract Infection and the Effects of FimK on Type 1 Pilus Expression." Infection and Immunity 76, no. 7 (2008): 3337–45. http://dx.doi.org/10.1128/iai.00090-08.

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ABSTRACT Klebsiella pneumoniae is an important cause of urinary tract infection (UTI), but little is known about its pathogenesis in vivo. The pathogenesis of the K. pneumoniae cystitis isolate TOP52 was compared to that of the uropathogenic Escherichia coli (UPEC) isolate UTI89 in a murine cystitis model. Bladder and kidney titers of TOP52 were lower than those of UTI89 at early time points but similar at later time points. TOP52, like UTI89, formed biofilm-like intracellular bacterial communities (IBCs) within the murine bladder, albeit at significantly lower levels than UTI89. Additionally,

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19

A, Acero-Lopez, Schell P, Corredig M, and Alexander M. "Characterization of lactoferrin oil-in-water emulsions and their stability in recombined milk." Journal of Dairy Research 77, no. 4 (2010): 445–51. http://dx.doi.org/10.1017/s0022029910000622.

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Emulsions were prepared with 20% soy oil and different concentrations of lactoferrin, and tested at pH values from 3 to 7·5. The stability of the emulsions decreased as the pH got closer to the isoelectric point of the protein. A concentration of 1% lactoferrin was determined to be sufficient to provide full coverage of the emulsion droplets. Lactoferrin-stabilized emulsions were then prepared in water at pH 6·6 and their behaviour when added to reconstituted milk was studied. It was observed that lactoferrin emulsions were stable when reconstituted in milk, but they showed aggregation when di

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20

Slupianek, Artur, Yashodhara Dasgupta, Shu-yue Ren, Kimberly Cramer, and Tomasz Skorski. "Targeting Phosphotyrosine-315 In RAD51 Recombinase to Prevent BCR-ABL1 - Mediated Unfaithful Homeologous Recombination Repair." Blood 116, no. 21 (2010): 1190. http://dx.doi.org/10.1182/blood.v116.21.1190.1190.

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Abstract Abstract 1190 Background: CD34+ chronic myeloid leukemia (CML) stem/progenitor cells from chronic phase (CML-CP) and blast phase (CML-BP) and cell lines transformed by non-mutated BCR-ABL1 kinase or tyrosine kinase inhibitor (TKI)-resistant mutants contain numerous DNA double-strand breaks (DSBs) induced by reactive oxygen species (ROS) (Nowicki et al., Blood, 2006; Cramer et al., Cancer Res., 2008). DSBs may cause apoptosis if not repaired or chromosomal aberrations if repaired unfaithfully. We reported that numerous ROS and radiation induced DSBs generated chromosomal aberrations in

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21

Cao, Xing Zhong, Er Yang Lu, Shuo Xue Jin, et al. "Irradiation Induced Defects by Fe-Ion in 316L Stainless Steel Studied by Positron Annihilation Spectroscopy." Defect and Diffusion Forum 373 (March 2017): 155–61. http://dx.doi.org/10.4028/www.scientific.net/ddf.373.155.

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Solution annealed type 316L austenitic stainless steels were irradiated using 2 MeV Fe ions at room temperature. The implanted fluences were 2×1012 ions/cm2 and 1×1013 ions/cm2, respectively. Variable mono-energetic positron beam was performed to characterize the evolution of microstructure and irradiation induced defects. Results show that large amount of vacancy defects formed after heavy ion irradiation. In which, some of mono-vacancies might migrate to form small-sized clusters at room temperature. After irradiation, implanted Fe atoms mainly be interstitials atoms, but some Fe atoms might

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22

Leroux, Killian, Jean-Claude Guillemin, and Lahouari Krim. "Organic residues in astrophysical ice analogues: Thermal processing of hydrogenated glyoxal ices under interstellar conditions." Monthly Notices of the Royal Astronomical Society 504, no. 2 (2021): 2181–89. http://dx.doi.org/10.1093/mnras/stab951.

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ABSTRACT Organic residues are considered as part of the chemical composition of the interstellar dust grains. They are formed under the extreme conditions of the interstellar medium and play an important role in exobiology. They may contain pre-biotic organic species such as amino acids, constituents of proteins and building blocks of DNA and RNA, key elements of life. By investigating the formation of organic residues in an astrophysical context, many groups have been focusing in the UV irradiation and subsequent warm-up of astrophysical ice analogues. This aims to suggest that organic residu

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23

Chen, Wenliang, and Sue Jinks-Robertson. "The Role of the Mismatch Repair Machinery in Regulating Mitotic and Meiotic Recombination Between Diverged Sequences in Yeast." Genetics 151, no. 4 (1999): 1299–313. http://dx.doi.org/10.1093/genetics/151.4.1299.

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Abstract Nonidentical recombination substrates recombine less efficiently than do identical substrates in yeast, and much of this inhibition can be attributed to action of the mismatch repair (MMR) machinery. In this study an intron-based inverted repeat assay system has been used to directly compare the rates of mitotic and meiotic recombination between pairs of 350-bp substrates varying from 82% to 100% in sequence identity. The recombination rate data indicate that sequence divergence impacts mitotic and meiotic recombination similarly, although subtle differences are evident. In addition t

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24

Schmidt, Katherine J., Kristen E. Beck, and Dennis W. Grogan. "UV Stimulation of Chromosomal Marker Exchange in Sulfolobus acidocaldarius: Implications for DNA Repair, Conjugation and Homologous Recombination at Extremely High Temperatures." Genetics 152, no. 4 (1999): 1407–15. http://dx.doi.org/10.1093/genetics/152.4.1407.

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Abstract The hyperthermophilic archaeon Sulfolobus acidocaldarius exchanges and recombines chromosomal markers by a conjugational mechanism, and the overall yield of recombinants is greatly increased by previous exposure to UV light. This stimulation was studied in an effort to clarify its mechanism and that of marker exchange itself. A variety of experiments failed to identify a significant effect of UV irradiation on the frequency of cell pairing, indicating that subsequent steps are primarily affected, i.e., transfer of DNA between cells or homologous recombination. The UV-induced stimulati

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Tkachenko, Nikolai V., Visa Vehmanen, Alexander Efimov, Alexander S. Alekseev, and Helge Lemmetyinen. "Vectorial photoinduced electron transfer of phytochlorin-fullerene systems in Langmuir-Blodgett films." Journal of Porphyrins and Phthalocyanines 07, no. 04 (2003): 255–63. http://dx.doi.org/10.1142/s1088424603000343.

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Molecular systems capable of photoinduced vectorial electron transfer (ET) were assembled using the Langmuir-Blodgett technique. The active part of the systems consisted of phytochlorin-fullerene donor-acceptor layers and polythiophene secondary donor layers. Such structures can promote multi-step inter-layer ET in a direction determined by the film architecture. Transient photovoltage and photocurrent were studied and ET rates and efficiencies were determined. The primary intramolecular charge separated state was formed upon photoexcitation in less than 1 ns and it recombined in approximately

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26

Shintani, Yoshizumi, Hiroshi Yotsuyanagi, Kyoji Moriya, et al. "Induction of apoptosis after switch-on of the hepatitis B virus X gene mediated by the Cre/loxP recombination system." Journal of General Virology 80, no. 12 (1999): 3257–65. http://dx.doi.org/10.1099/0022-1317-80-12-3257.

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The HBx protein of hepatitis B virus is a multifunctional protein that is implicated in the pathogenesis of hepatocellular carcinoma by regulating gene transcription, causing cell proliferation and, as shown recently, inducing cell death. However, analysis of the effects of HBx in stable cultured cell clones has been hampered because only cell lines that adapted to the effects of HBx were selected during the establishment of cell clones. Here, we describe a system in which transcription of the X gene of hepatitis B virus is switched on by the use of the site-specific Cre recombinase. Two human

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Martï¿½nez-Granero, Francisco, Rafael Rivilla, and Marta Martï¿½n. "Rhizosphere Selection of Highly Motile Phenotypic Variants of Pseudomonas fluorescens with Enhanced Competitive Colonization Ability." Applied and Environmental Microbiology 72, no. 5 (2006): 3429–34. http://dx.doi.org/10.1128/aem.72.5.3429-3434.2006.

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ABSTRACT Phenotypic variants of Pseudomonas fluorescens F113 showing a translucent and diffuse colony morphology show enhanced colonization of the alfalfa rhizosphere. We have previously shown that in the biocontrol agent P. fluorescens F113, phenotypic variation is mediated by the activity of two site-specific recombinases, Sss and XerD. By overexpressing the genes encoding either of the recombinases, we have now generated a large number of variants (mutants) after selection either by prolonged laboratory cultivation or by rhizosphere passage. All the isolated variants were more motile than t

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Király, Lorant, June E. Bourque, and James E. Schoelz. "Temporal and Spatial Appearance of Recombinant Viruses Formed Between Cauliflower Mosaic Virus (CaMV) and CaMV Sequences Present in Transgenic Nicotiana bigelovii." Molecular Plant-Microbe Interactions® 11, no. 4 (1998): 309–16. http://dx.doi.org/10.1094/mpmi.1998.11.4.309.

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Cauliflower mosaic virus (CaMV) strain CM1841 is able to recombine with a CaMV transgene sequence present in Nicotiana bigelovii. In the present study we have characterized the temporal and spatial appearance of recombinant viruses formed between CM1841 and the transgene within individual transgenic plants. CM1841 infections were initiated by mechanical inoculation and by agro-inoculation to nontransformed N. bigelovii and transgenic N. bigelovii that expressed the gene VI product of CaMV strain D4. In agroinoculated transgenic plants, inoculated leaf tissue turned necrotic around the point of

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Somwan, Siripong, Khem Chirapatpimol, Apichart Limpichaipanit, Komsanti Chokethawai, and Athipong Ngamjarurojana. "Optical Interferometric Technique for Induced Strain Ferroelectric Loop Study at Low Frequency." Advanced Materials Research 936 (June 2014): 110–14. http://dx.doi.org/10.4028/www.scientific.net/amr.936.110.

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Michelson interferometric technique was modified in the setup and used to measure very small changes in optical path length for electric field induced-strain phenomena. Induced-strain is established in the sample when the beam of light propagating through the sample surface undergoes a shift in phase, which in turn leads to a change in the intensity of the interference pattern formed where the reference and probing beams recombine. In this work the electric field induced-strain was measured as a function of electric field (s-E loop) and polarization (s-P loop) of PbMg1/3Nb2/3O3 (PMN) and 0.7Pb

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Andersen, Heidi Gade, and Curt Wentrup. "Pyrido[1,2-a]pyrimidinones and Thiazolo[3,2-a]pyrimidinones: Precursors for Pyridyl- and Thiazolyliminopropadienones, R - N=C=C=C=O." Australian Journal of Chemistry 65, no. 2 (2012): 105. http://dx.doi.org/10.1071/ch11414.

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2-Pyridyliminopropadienone 6 is formed together with 2-aminopyridine on flash vacuum thermolysis (FVT) of 2-pyridylamino-pyridopyrimidinone 16a and observed by Ar matrix IR spectroscopy, but the two products recombine on warming to room temperature to regenerate the starting material. FVT of the picolinylamino-pyridopyrimidinones 16b and 16c generated mixtures of pyridyliminopropadienone 6 and picolinyliminopropadienones 19b and 19c, respectively. These reactions can be understood in terms of fragmentation of the open-chain bis(2-pyridylamino)methyleneketene intermediates 20 or the thermal int

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Lambert, Nicholas A. "On Standards: A Reply to Christopher Bell." War in History 19, no. 2 (2012): 217–40. http://dx.doi.org/10.1177/0968344511432977.

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During his first term as first sea lord (1904–10), Admiral Sir John Fisher set in motion the intellectual, organizational, and technological forces that, in very rapid order, combined and recombined to generate new approaches to British naval policy. Like most if not all ‘revolutions’ in technology and warfare, this one did not start and end neatly under one man’s control, but took on a life of its own. Not all elements of his original vision advanced as far as others. Yet, however variable the direction and extent of the revolution at particular times, in retrospect we can say indubitably tha

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Radman, Miroslav. "Mismatch repair and the fidelity of genetic recombination." Genome 31, no. 1 (1989): 68–73. http://dx.doi.org/10.1139/g89-014.

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Two modes of mismatch repair are known to operate in bacteria: long-patch mismatch repair and very short patch mismatch repair. Very short patch mismatch repair systems act on a specific mismatch by conserving only one base pair. Therefore, when very short patch mismatch repair acts on heteroduplex recombination intermediates, it hyper-recombines specific markers by creating patchwork sequences, i.e., apparent multiple exchange events, on the repaired strand. Long-patch mismatch repair is antirecombinagenic, apparently by decomposing heteroduplex DNA or aborting its formation whenever well-rec

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Giesen, Klaus-Gerd. "L'Europe, l'évaluation technologique et la régulation fractale mondiale." Études internationales 26, no. 3 (2005): 479–502. http://dx.doi.org/10.7202/703488ar.

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The study attempts to make a theoretically informed analysis of technology assessment (TA) as part of postfordist global governance. It focuses first on the FAST programme of the EC, designed to regulate the relationship between producers of new technologies (industry, states) and civil society. The author shows that this regulation, based on the expertise of the social sciences, is largely asymmetrical in favour of the former and an attempt to engineer social consensus at the supranational level. The focus shifts then downwards to the numerous national and regional TA institutions in Western

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Tylenda, R. "Resonance Line Profiles from Radial Accretion Flows." International Astronomical Union Colloquium 103 (1988): 127–28. http://dx.doi.org/10.1017/s0252921100103276.

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A compact star in a detached binary system can accrete the matter from the stellar wind of the companion. In this case a more or less radial accretion flow is formed. By analogy to stellar winds from early type stars it is usually believed that the accretion of this sort should produce inverse P-Cygni profiles in resonance lines. However, there are physical differences between the wind and the radial accretion which can alter the outgoing profile significantly.The matter outflowing from an early type star cools off very fast due to the adiabatic expansion. However, it remains highly ionized as

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Skamaki, Kalliopi, Stephane Emond, Matthieu Chodorge, et al. "In vitro evolution of antibody affinity via insertional scanning mutagenesis of an entire antibody variable region." Proceedings of the National Academy of Sciences 117, no. 44 (2020): 27307–18. http://dx.doi.org/10.1073/pnas.2002954117.

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We report a systematic combinatorial exploration of affinity enhancement of antibodies by insertions and deletions (InDels). Transposon-based introduction of InDels via the method TRIAD (transposition-based random insertion and deletion mutagenesis) was used to generate large libraries with random in-frame InDels across the entire single-chain variable fragment gene that were further recombined and screened by ribosome display. Knowledge of potential insertion points from TRIAD libraries formed the basis of exploration of length and sequence diversity of novel insertions by insertional-scannin

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Sciochetti, Stephen A., Patrick J. Piggot, David J. Sherratt, and Garry Blakely. "The ripX Locus of Bacillus subtilis Encodes a Site-Specific Recombinase Involved in Proper Chromosome Partitioning." Journal of Bacteriology 181, no. 19 (1999): 6053–62. http://dx.doi.org/10.1128/jb.181.19.6053-6062.1999.

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ABSTRACT The Bacillus subtilis ripX gene encodes a protein that has 37 and 44% identity with the XerC and XerD site-specific recombinases of Escherichia coli. XerC and XerD are hypothesized to act in concert at the dif site to resolve dimeric chromosomes formed by recombination during replication. Cultures of ripX mutants contained a subpopulation of unequal-size cells held together in long chains. The chains included anucleate cells and cells with aberrantly dense or diffuse nucleoids, indicating a chromosome partitioning failure. This result is consistent with RipX having a role in the resol

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Amin, A., H. Roca, K. Luetke, and P. D. Sadowski. "Synapsis, strand scission, and strand exchange induced by the FLP recombinase: analysis with half-FRT sites." Molecular and Cellular Biology 11, no. 9 (1991): 4497–508. http://dx.doi.org/10.1128/mcb.11.9.4497.

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We have used a previously described cross-linking assay and half-FRT site substrates to examine the requirements for synapsis, strand exchange, and strand scission. The cross-linking assay showed that the minimum functional FRT site needed for synapsis contains two inverted FLP-binding elements surrounding an 8-bp core. This indicates that four FLP molecules interact with four binding elements in a synaptic complex. The analysis using half-sites showed that the enzyme can catalyze efficient strand exchange between a half-site and the intact FRT site. The reaction occurred only if the half-site

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Amin, A., H. Roca, K. Luetke, and P. D. Sadowski. "Synapsis, strand scission, and strand exchange induced by the FLP recombinase: analysis with half-FRT sites." Molecular and Cellular Biology 11, no. 9 (1991): 4497–508. http://dx.doi.org/10.1128/mcb.11.9.4497-4508.1991.

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We have used a previously described cross-linking assay and half-FRT site substrates to examine the requirements for synapsis, strand exchange, and strand scission. The cross-linking assay showed that the minimum functional FRT site needed for synapsis contains two inverted FLP-binding elements surrounding an 8-bp core. This indicates that four FLP molecules interact with four binding elements in a synaptic complex. The analysis using half-sites showed that the enzyme can catalyze efficient strand exchange between a half-site and the intact FRT site. The reaction occurred only if the half-site

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Fan, Hsiu-Fang, Bo-Yu Su, Chien-Hui Ma, Paul A. Rowley, and Makkuni Jayaram. "A bipartite thermodynamic-kinetic contribution by an activating mutation to RDF-independent excision by a phage serine integrase." Nucleic Acids Research 48, no. 12 (2020): 6413–30. http://dx.doi.org/10.1093/nar/gkaa401.

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Abstract Streptomyces phage ϕC31 integrase (Int)—a large serine site-specific recombinase—is autonomous for phage integration (attP x attB recombination) but is dependent on the phage coded gp3, a recombination directionality factor (RDF), for prophage excision (attL x attR recombination). A previously described activating mutation, E449K, induces Int to perform attL x attR recombination in the absence of gp3, albeit with lower efficiency. E449K has no adverse effect on the competence of Int for attP x attB recombination. Int(E449K) resembles Int in gp3 mediated stimulation of attL x attR reco

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Segall, A. M., and J. R. Roth. "Recombination between homologies in direct and inverse orientation in the chromosome of Salmonella: intervals which are nonpermissive for inversion formation." Genetics 122, no. 4 (1989): 737–47. http://dx.doi.org/10.1093/genetics/122.4.737.

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Abstract Sequences placed in inverse order at particular chromosome sites (permissive) recombine to generate an inversion; the same sequences, placed at other sites (nonpermissive) interact recombinationally but do not form the expected inversion recombinants. We have investigated the events that occur between sequences at nonpermissive sites. Genetically marked lac operons in inverse order were placed at nonpermissive sites in a single chromosome and Lac+ recombinants were selected. No inversions were formed. The Lac+ recombinants recovered include double-recombinant types in which informatio

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Berry, M. V. "Wave dislocation lines threading interferometers." Proceedings of the Royal Society A: Mathematical, Physical and Engineering Sciences 463, no. 2083 (2007): 1697–711. http://dx.doi.org/10.1098/rspa.2007.1842.

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In interferometers where a wave is divided into two beams that propagate along separate branches before being recombined, the closed circuit formed by the two branches must be threaded by wave dislocation lines. For a large class of interferometers, it is shown that the (signed) dislocation number, defined in a suitable asymptotic sense, jumps by +1 as the phase difference between the beams increases by 2 π . The argument is based on the single-valuedness of the wave function in the branches and leaking between them. In some cases, the jumps occur when the phase difference is an odd multiple o

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Mohan, William S., Elisabeth Scheer, Olivia Wendling, Daniel Metzger, and Làszlò Tora. "TAF10 (TAFII30) Is Necessary for TFIID Stability and Early Embryogenesis in Mice." Molecular and Cellular Biology 23, no. 12 (2003): 4307–18. http://dx.doi.org/10.1128/mcb.23.12.4307-4318.2003.

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ABSTRACT TAF10 (formerly TAFII30), is a component of TFIID and the TATA box-binding protein (TBP)-free TAF-containing complexes (TFTC/PCAF/STAGA). To investigate the physiological function of TAF10, we disrupted its gene in mice by using a Cre recombinase/LoxP strategy. Interestingly, no TAF10−/− animals were born from intercrosses of TAF10+/− mice, indicating that TAF10 is required for embryogenesis. TAF10−/− embryos developed to the blastocyst stage, implanted, but died shortly after ca. 5.5 days postcoitus. Surprisingly, trophoblast cells from TAF10−/− blastocysts were viable, whereas inner

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Cheah, Pike-See, Shilpa Prabhakar, David Yellen, et al. "Gene therapy for tuberous sclerosis complex type 2 in a mouse model by delivery of AAV9 encoding a condensed form of tuberin." Science Advances 7, no. 2 (2021): eabb1703. http://dx.doi.org/10.1126/sciadv.abb1703.

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Tuberous sclerosis complex (TSC) results from loss of a tumor suppressor gene - TSC1 or TSC2, encoding hamartin and tuberin, respectively. These proteins formed a complex to inhibit mTORC1-mediated cell growth and proliferation. Loss of either protein leads to overgrowth lesions in many vital organs. Gene therapy was evaluated in a mouse model of TSC2 using an adeno-associated virus (AAV) vector carrying the complementary for a “condensed” form of human tuberin (cTuberin). Functionality of cTuberin was verified in culture. A mouse model of TSC2 was generated by AAV-Cre recombinase disruption o

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Kuan, C. T., S. K. Liu, and I. Tessman. "Excision and transposition of Tn5 as an SOS activity in Escherichia coli." Genetics 128, no. 1 (1991): 45–57. http://dx.doi.org/10.1093/genetics/128.1.45.

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Abstract Excision and transposition of the Tn5 element in Escherichia coli ordinarily appear to occur by recA-independent mechanisms. However, recA(Prtc) genes, which encode RecA proteins that are constitutively activated to the protease state, greatly enhanced excision and transposition; both events appeared to occur concomitantly and without destruction of the donor DNA. The recombinase function of the RecA protein was not required. Transposition was accompanied by partial, and occasionally full, restoration of the functional integrity of the gene vacated by the excised Tn5. The stimulation

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Kung, Stephanie H., and Rodrigo P. P. Almeida. "Natural Competence and Recombination in the Plant Pathogen Xylella fastidiosa." Applied and Environmental Microbiology 77, no. 15 (2011): 5278–84. http://dx.doi.org/10.1128/aem.00730-11.

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ABSTRACTHomologous recombination is one of many forces contributing to the diversity, adaptation, and emergence of pathogens. For naturally competent bacteria, transformation is one possible route for the acquisition of novel genetic material. This study demonstrates thatXylella fastidiosa, a generalist bacterial plant pathogen responsible for many emerging plant diseases, is naturally competent and able to homologously recombine exogenous DNA into its genome. Several factors that affect transformation and recombination efficiencies, such as nutrient availability, growth stage, and methylation

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Henry, R. B. C., and H. L. Shipman. "The Origin of the Zanstra Discrepancy: UV Excess in the Central Star Continuum?" Symposium - International Astronomical Union 131 (1989): 305. http://dx.doi.org/10.1017/s0074180900138537.

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The temperature of a planetary nebula central star (CPN) may be determined by observing the nebular flux in an H I or He II recombination line and the stellar flux in a continuum band. The former measures the integrated UV stellar continuum blueward of the ionization edge of the recombined ion. By assuming a continuum shape (usually a blackbody), the ratio of these two fluxes yields an effective temperature for the CPN. This particular method, first introduced by Zanstra (1931), has an advantage over others in that the observables are relatively straightforward to obtain. However, this method

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KLIMOV, PAVEL B., BARRY C. OCONNOR, and ALEXANDER A. KHAUSTOV. "Naiadacarus sidorchukae sp. n. (Acariformes: Acaridae) from Western Siberia closely related to a North American species, with a new generic synonymy." Zootaxa 4647, no. 1 (2019): 441–56. http://dx.doi.org/10.11646/zootaxa.4647.1.28.

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A new species of acarid mite, Naiadacarus sidorchukae sp. n., is described from phoretic deutonymphs collected from a European spruce bark beetle, Ips typographus, pheromone trap in Western Siberia, Russia. Morphologically, the new species is very similar to the North American species, Naiadacarus fashingi, with the only diagnostic character being the shorter tibial solenidion φ II in the former species. Unlike some Naiadacarus species, which are truly aquatic, Naiadacarus sidorchukae probably does not inhabit fully aquatic habitats. This species and other presumably non-aquatic taxa, may repr

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Tickle, C., A. Crawley, and J. Farrar. "Retinoic acid application to chick wing buds leads to a dose-dependent reorganization of the apical ectodermal ridge that is mediated by the mesenchyme." Development 106, no. 4 (1989): 691–705. http://dx.doi.org/10.1242/dev.106.4.691.

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Local application of retinoic acid to wing buds of chick embryos leads to dose- and position-dependent changes in the pattern of cellular differentiation. Early effects of retinoid treatment on the apical ectodermal ridge coordinate pattern changes and morphogenesis. The length of the apical ridge increases when additional digits will form but decreases when digits are lost. These changes in length can be understood in terms of a threshold response to the local retinoid concentration that results in either disappearance or maintenance of the ridge (Lee & Tickle, J. Embryol. exp. Morph. 90,

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Xiong, Ling, Xiaole L. Chen, Hannah R. Silver, Noreen T. Ahmed та Erica S. Johnson. "Deficient SUMO Attachment to Flp Recombinase Leads to Homologous Recombination-dependent Hyperamplification of the Yeast 2 μm Circle Plasmid". Molecular Biology of the Cell 20, № 4 (2009): 1241–51. http://dx.doi.org/10.1091/mbc.e08-06-0659.

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Many Saccharomyces cerevisiae mutants defective in the SUMO pathway accumulate elevated levels of the native 2 μm circle plasmid (2 μm). Here we show that accumulation of 2 μm in the SUMO pathway mutants siz1Δ siz2Δ, slx5Δ, and slx8Δ is associated with formation of an aberrant high-molecular-weight (HMW) form of 2 μm. Characterization of this species from siz1Δ siz2Δ showed that it contains tandem copies of the 2 μm sequence as well as single-stranded DNA. Accumulation of this species requires both the 2 μm–encoded Flp recombinase and the cellular homologous recombination repair (HRR) pathway.

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Ono, Jasmine, and Duncan Greig. "A Saccharomyces paradox: chromosomes from different species are incompatible because of anti-recombination, not because of differences in number or arrangement." Current Genetics 66, no. 3 (2019): 469–74. http://dx.doi.org/10.1007/s00294-019-01038-x.

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AbstractMany species are able to hybridize, but the sterility of these hybrids effectively prevents gene flow between the species, reproductively isolating them and allowing them to evolve independently. Yeast hybrids formed by Saccharomyces cerevisiae and Saccharomyces paradoxus parents are viable and able to grow by mitosis, but they are sexually sterile because most of the gametes they make by meiosis are inviable. The genomes of these two species are so diverged that they cannot recombine properly during meiosis, so they fail to segregate efficiently. Thus most hybrid gametes are inviable

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