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Journal articles on the topic 'Fractionated extracts'

Author: Grafiati

Published: 4 June 2025

Last updated: 15 July 2025

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1

Fadillah, Wendi Nurul, Nampiah Sukarno, Dyah Iswantini, Min Rahminiwati, Novriyandi Hanif, and Mashuri Waite. "In Vitro Pancreatic Lipase Inhibition by Marine Fungi Purpureocillium lilacinum Associated with Stylissa sp. Sponge as Anti-obesity Agent." HAYATI Journal of Biosciences 29, no. 1 (2021): 76–86. http://dx.doi.org/10.4308/hjb.29.1.76-86.

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This study aimed to evaluate the potential of marine fungus Purpureocillium lilacinum isolated from an Indonesian marine sponge Stylissa sp. as an anti-obesity agent through pancreatic lipase inhibition assay. The fungus was identified as P. lilacinum through morphological and molecular characteristics. The fungal extract’s inhibition activity and kinetics were evaluated using spectrophotometry and Lineweaver-Burk plots. Ethyl acetate and butanol were used for extraction. Both extracts showed pancreatic lipase inhibition in a concentration-dependent manner. Both crude extracts were then fracti

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Ojongbede Onose, Naiho Alexender Obidike, Nwogueze Bartholomew Chukwuebuka, et al. "Invitro Membrane Stabilizing Potentials Of Fractionates Of Ethanolic Extract of Carica Papaya Leaf." International Journal of Research in Pharmaceutical Sciences 12, no. 4 (2021): 2335–41. http://dx.doi.org/10.26452/ijrps.v12i4.4865.

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Invitro membrane-stabilizing potentials of fractionates of ethanolic extract of Carica Papaya leaf was investigated in this study. The soxhlet extraction method was used to extract the plant, fractionated with 6 different solvents to give 6 different fractions (hexane, ethyl acetate, chloroform, butanol, methanol benzene). Hbss red blood cells samples were obtained from non-crises state sickle cell patients from Eku Baptist hospital Abraka Delta State, Nigeria. These tests involved the use of positive (p-hydroxy benzoic acid 5ug/ml) and negative controls (normal saline) for membrane stability

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Curtis, Rachael M., Heng-Shan Wang, Xuan Luo, Erika B. Dugo, Jacqueline J. Stevens, and Paul B. Tchounwou. "Fractionated Leaf Extracts of Ocimum gratissimum Inhibit the Proliferation and Induce Apoptosis of A549 Lung Adenocarcinoma Cells." Nutrients 16, no. 16 (2024): 2737. http://dx.doi.org/10.3390/nu16162737.

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Previous in vitro studies in our laboratory demonstrated that ethyl acetate (P2) and water- soluble (PS/PT1) fractionated leaf extracts of Ocimum gratissimum inhibit the proliferation of prostate cancer cells. It has been reported that the crude aqueous extract induces apoptosis in lung adenocarcinoma cells; however, the efficacy of the fractionated extracts against these cells remains unclear. In the present study, we hypothesized that the ability of the fractionated extracts to inhibit proliferation and induce apoptosis is associated with the activation of pro-apoptotic proteins and inductio

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Bakri, Husna Hazirah, Syarifah Nur Syed Abdul Rahman, Zarith Safinaz Dol Bakri, Elly Munadziroh, and Wan Himratul Aznita Wan Harun. "Antimicrobial activity of Ruta angustifolia L. Pers against periodontal pathogen: Porphyromonas gingivalis." PeerJ 12 (December 18, 2024): e18751. https://doi.org/10.7717/peerj.18751.

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Background Porphyromonas gingivalis is widely recognised as a periodontal pathogen. In recent years, there has been growing interest in the use of medicinal plant extracts as alternative treatments for periodontitis to combat the emergence of antibiotic-resistant bacteria. Ruta angustifolia L. Pers has been traditionally used to treat various ailments, including oral bacterial infections. However, the antimicrobial potential of R. angustifolia extracts against the periodontal pathogen P. gingivalis remains unexplored. Hence, the aim of this study was to investigate the antimicrobial activity o

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Widiyanti, Prihartini, Bambang Prajogo, and Ni Putu Ermi Hikmawati. "CYTOTOXICITY OF JUSTICIA GENDARUSSA BURM F. LEAF EXTRACTS ON MOLT-4 CELL." Indonesian Journal of Tropical and Infectious Disease 6, no. 1 (2016): 24. http://dx.doi.org/10.20473/ijtid.v6i1.1207.

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Justicia gendarussa Burm f. (Acanthaceae) is known for its activity as a male contraceptive and anti-HIV properties. The present study was designed to evaluate extracts of J. gendarussa for cytotoxicity activity against MOLT-4 cells. The cytotoxic activity of the fractionated-extract and 70% ethanol extracts of J. gendarussa leaves on MOLT-4 cells were evaluated using a WST-1 assay. The treatment cells, control cells without treatment and control media were also tested in duplicate. The absorbance was measured at a wavelength of 450 nm using a microplate absorbance reader (Bio-Rad). The averag

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Soundararajan, Ramani, Punit Prabha, Umesh Rai, and Aparna Dixit. "Antileukemic Potential ofMomordica charantiaSeed Extracts on Human Myeloid Leukemic HL60 Cells." Evidence-Based Complementary and Alternative Medicine 2012 (2012): 1–10. http://dx.doi.org/10.1155/2012/732404.

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Momordica charantia(bitter gourd) has been used in the traditional system of medicine for the treatment of various diseases. Anticancer activity ofM. charantiaextracts has been demonstrated by numerousin vitroandin vivostudies. In the present study, we investigated the differentiation inducing potential of fractionatedM. charantiaseed extracts in human myeloid HL60 cells. We found that the HL60 cells treated with the fractionated seed extracts differentiated into granulocytic lineage as characterized by NBT staining, CD11b expression, and specific esterase activity. The differentiation inducin

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7

Rodwattanagul, Soraya, Pathomwat Wongrattanakamon, Siripat Chaichit, et al. "Exploring the antioxidant, antiglycation, and anti-inflammatory potential of Oroxylum indicum stem bark extracts." PLOS One 20, no. 6 (2025): e0325795. https://doi.org/10.1371/journal.pone.0325795.

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Degenerative diseases occur when humans suffer from oxidative stress, glycation and prolonged inflammation. This study explores the antioxidant, antiglycation, and anti-inflammatory effects of extracts of Oroxylum indicum, a plant used in traditional medicines in Asia. Several extracts from its stem bark were obtained using hexane, ethyl acetate, and ethanol as extraction solvents. The extracts were analyzed using high-performance liquid chromatography (HPLC). The HPLC chromatograms showed that the different O. indicum extracts contained three major flavonoid compounds, namely baicalein, chrys

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8

Kerdchan, K., and P. Srihanam. "Fractionation of Sugarcane Extracts and Determination of Phenolic Compounds and their Antioxidant Activity." Asian Journal of Chemistry 34, no. 2 (2022): 284–88. http://dx.doi.org/10.14233/ajchem.2022.23474.

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Crude ethanolic extracts of four cultivars of sugarcane (Saccharum officinarum L.) were firstly prepared and then fractionated using silica gel column chromatography before the determination of their oxidative substances. The fractionated extracts were then tested for antioxidant activity by various assays. Finally, high-performance liquid chromatography (HPLC) was applied for the quantitative determination of the individual phenolic compounds. Sub-fraction 2 has the highest phytochemical contents as well as antioxidant activity. All tested phytochemicals had positively correlated to antioxida

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Kubheka, Mbali X., Sizwe Ndlovu, and Nompumelelo P. Mkhwanazi. "Anti-HIV Activity and Immunomodulatory Properties of Fractionated Crude Extracts of Alternaria alternata." Microorganisms 12, no. 6 (2024): 1150. http://dx.doi.org/10.3390/microorganisms12061150.

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Developing new anti-human immunodeficiency virus (HIV) drug candidates that target different sites in HIV-1 replication, with better resistance profiles and lower drug toxicity, is essential to eradicating HIV. This study investigated the potential of fractionated crude extracts of Alternaria alternata as immunomodulatory or anti-HIV drug candidates. Solid-phase extraction (SPE) was used to fractionate A. alternata PO4PR2 using three different columns: MAX (Mixed-mode, strong Anion-eXchange), MCX (Mixed-mode, strong Cation-eXchange), and HLB (Hydrophilic–Lipophilic Balance) with methanol gradi

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Wongkattiya, Nalin, Phanchana Sanguansermsri, Chareeporn Akawatchai, Ian Fraser, and Donruedee Sanguansermsri. "Antibacterial, Antioxidant, and Cytotoxic Properties of Eclipta prostrata Extracts." HAYATI Journal of Biosciences 31, no. 6 (2024): 1165–72. http://dx.doi.org/10.4308/hjb.31.6.1165-1172.

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Eclipta prostrata is traditionally used in Asian medicine to treat skin diseases. Given the side effects and bacterial resistance of conventional treatments, especially in aging populations, alternative therapies are needed. This study assesses the antibacterial, antioxidant, and cytotoxic properties of E. prostrata extracts, focusing on optimizing extraction and fractionation methods for better efficacy. Dried whole plants were extracted with ethanol. Antibacterial activity was tested against Staphylococcus aureus, MRSA, S. epidermidis, and Pseudomonas aeruginosa using broth microdilution to

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Nguyen, Thuan Thi Minh, and Nguyen Thi Thao Le. "Inhibitory effect of medicinal plants on in vitro human peripheral blood mononuclear cell proliferation, interleukin-2 secretion and antioxidant activity." MedPharmRes 5, no. 1 (2021): 22–29. http://dx.doi.org/10.32895/ump.mpr.5.1.5.

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Many plant parts have been widely used in the treatment of immune diseases in Vietnam, yet just few of them are known about their mechanism of action. The goal of this study was to investigate the in vitro inhibitory effects of in vitro inhibitory effect of crude ethanol extracts and fractions of thirteen medicinal plants on proliferation of human peripheral blood mononuclear cells (PBMCs), interleukin-2 secretion and antioxidant activity. PBMCs were extracted from the whole blood of healthy volunteers. The effects of thirteen crude extracts in ethanol and twenty four fractionated extracts in

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Joe, Ga-Hyun, Masafumi Masuoka, Ryosuke Reisen, Seiya Tanaka, and Hiroki Saeki. "Development of a Sequential Fractionation-and-Recovery Method for Multiple Anti-Inflammatory Components Contained in the Dried Red Alga Dulse (Palmaria palmata)." Marine Drugs 21, no. 5 (2023): 276. http://dx.doi.org/10.3390/md21050276.

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A separation process was established to sequentially fractionate and recover three anti-inflammatory components derived from sugars, phycobiliprotein, and chlorophyll from the hot-air-dried thalli of the red alga dulse (Palmaria palmata). The developed process consisted of three steps, without the use of organic solvents. In Step I, the sugars were separated by disrupting the cell wall of the dried thalli with a polysaccharide-degrading enzyme, and a sugar-rich extract (E1) was obtained by precipitating the other components, which were simultaneously eluted by acid precipitation. In Step II, t

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TAKAGI, Yukihiko, Ryouhei KITANI, Yukio KATO, et al. "Mutagenicity of Extracts from Size Fractionated Soils." Journal of Environmental Chemistry 14, no. 2 (2004): 327–33. http://dx.doi.org/10.5985/jec.14.327.

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NAHEED, Neelum, Yamin BIBI, Sehrish IMRAN, et al. "Pharmacological profiling and phytochemical analysis of fractionated extracts of Euphorbia royleana." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 52, no. 4 (2024): 13711. https://doi.org/10.15835/nbha52413711.

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Euphorbia royleana belongs to family Euphorbiaceae, with great therapeutic potential. The present study is aimed to validate its traditional uses. GC-MS analysis of Euphorbia royleana crude and fractionated extracts were performed. Cytotoxicity was evaluated by Brine shrimp lethality (BSL) assay. Plant extract antioxidant activity was performed through in vitro multidimensional assays. Plant elemental analysis was performed through atomic absorption spectroscopy. In addition, the extract antibacterial activity against two gram negative i.e. Staphylococcus aureus, Xanthomonas campestris, and tw

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15

Ikpefan, E. O., J. O. Ikpefan, S. O. Ovbiagele, U. Ejienyafu, and C. B. Ojumah. "Euphorbia Graminea Jacq. (Euphorbiaceae): A Comparative Antimicrobial Evaluations of Stem and Root Extracts." Journal of Applied Sciences and Environmental Management 27, no. 2 (2023): 249–55. http://dx.doi.org/10.4314/jasem.v27i2.10.

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Antimicrobial resistance is a global issue. Euphorbia plants are used locally to treat microbial infections. This study examined the antimicrobial potential of Euphorbia graminea stem and root extracts. The stems and roots extracts of E .graminea were extracted using 80% methanol and tested for antimicrobial activity at concentrations between 4.69-300mg/mL against non-clinical isolates (S. aureus, E. coli, P. aeruginosa, C. albican, A. niger). The active roots extract was fractionated using vacuum liquid chromatographic fractionations (VLC) and the resulting fractions bulked and tested against

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Oyewusi, J. A., O. A. Oridupa, A. B. Saba, and I. K. Oyewusi. "Comparative <i>in-vitro</i> antioxidant studies of crude methanol extract and four solvent fractions of red cultivar <i>Allium cepa</i> l. bulbs." Nigerian Veterinary Journal 43, no. 3 (2023): 42–53. http://dx.doi.org/10.4314/nvj.v43i3.4.

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Oxidative stress-induced disease conditions are on the increase and there is need for natural sources of antioxidant for relieving stress. The crude extract of Allium cepa L. has been well published as a potential natural source of antioxidant. But there is inadequate information on the antioxidant activities of fractionated extracts of the plant. The in-vitro antioxidant capacity of crude extract of red cultivar A. cepa bulbs and its four solvent fractions were studied and compared using Nitric oxide radical inhibitory assay (NORIA), FRAP and DPPH antioxidant models. The results of the three

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Brun, Paola, Anna Piovan, Rosy Caniato, Vanessa Dalla Costa, Anthony Pauletto, and Raffaella Filippini. "Anti-Inflammatory Activities of Euglena gracilis Extracts." Microorganisms 9, no. 10 (2021): 2058. http://dx.doi.org/10.3390/microorganisms9102058.

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Dietary supplementation with nutrients able to control intestinal and systemic inflammation is of marketable interest. Indeed, gastrointestinal homeostasis plays a significant role in maintaining human health. In this setting, E. gracilis may sustain or promote human health, but the effects on the intestinal inflammatory milieu are not clear. In this study, we investigated the anti-inflammatory activity of E. gracilis and inferred possible mechanisms. Paramylon, crude, and fractionated extracts were obtained from E. gracilis grown in vitro. Phytoconstituents of the extracts were characterized

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18

Hartati, Sri, Chie Aoki, Muhammad Hanafi, Marissa Angelina, Pratiwi Soedarmono, and Hak Hotta. "Antiviral effect of Archidendron pauciflorum leaves extract to hepatitis C virus: An in vitro study in JFH-1 strain." Medical Journal of Indonesia 27, no. 1 (2018): 12–8. http://dx.doi.org/10.13181/mji.v27i1.2189.

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Background: Hepatitis C virus (HCV) is a leading cause of chronic liver diseases. Drug resistance to the regimen is also increasing. Hence, there is a need for new anti-HCV agents that are less toxic and more efficacious. The aim of this study is to evaluate the possibility of A. pauciflorum extracts can be a antiviral drug.Methods: Huh-7it cells were infected with the HCV genotype 2a strain JFH-I in the presence of methanol extracts of Archidenron pauciflorum. The methanol extract further partition used n-hexane, ethyl acetate, n-butanol, and water showed in which butanol extracts exerted the

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Ezekiel, Adewole, Yusuf Bashira, Ebitimitula Ogola-Emma, et al. "Phytochemicals profile and in-vitro antidiabetic potentials of fractionated extracts of Entada Africana and Leptadenia Hastata." ScienceRise: Pharmaceutical Science, no. 3(37) (June 30, 2022): 65–73. https://doi.org/10.15587/2519-4852.2022.255744.

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<strong>Aim: </strong>To confirm their use in the management of diabetes and to determine the numerous phytochemicals present that may be connected to the active performance of the plants, the fractionated extracts of Leptadenia hastata and Entada africana were subjected to an in vitro experiment. <strong>Material and methods:</strong> The plant leaves were dried, pulverized with a Sumeet CM/L 2128945 grinder, the particle size was 45.85 μm and extracted with methanol. The crude extracts were fractionated using a 30×8 cm diameter column and 60g of silica gel 60 F254 grade, u

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Chandra, Amar K., Neela De, and Shyamosree Roy Choudhury. "Effect of different doses of un-fractionated green and black tea extracts on thyroid physiology." Human & Experimental Toxicology 30, no. 8 (2010): 884–96. http://dx.doi.org/10.1177/0960327110382563.

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Tea is a rich source of polyphenolic flavonoids including catechins, which are thought to contribute to the health benefits of it. Flavonoids have been reported to have antithyroid and goitrogenic effect. The purpose of this study was to evaluate whether high doses of green and black tea have a harmful effect on thyroid physiology. Un-fractionated green and black tea extracts were administered orally to male rats for 30 days at doses of 1.25 g%, 2.5 g% and 5.0 g%. The results showed that green tea extract at 2.5 g% and 5.0 g% doses and black tea extract only at 5.0 g% dose have the potential t

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Holle, Mukhlish Jamal Musa, Hestri Dyah Puspitasari, Andaru Satryo, Wahyu Dewi Astuti Ningrum, Digdo Sudigyo, and Ardaning Nuriliani. "CYTOTOXIC EFFECT OF AQUADEST, ETHANOLIC, AND CHLOROFORM EXTRACTS OF Spathoglottis plicata Blume ON BREAST CANCER CELLS LINE (T47D CELLS)." KnE Life Sciences 2, no. 1 (2015): 64. http://dx.doi.org/10.18502/kls.v2i1.118.

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Breast cancer is one of cancer with high mortality. This cancer not only attacks women, but also men. Indonesia has many plants which potential as anticancer, such as orchids. Spathoglottis plicata is one of the orchid species that abundant in Indonesia and has a lot of antioxidant compounds which is guessed have anticancer properties. The objectives of this study were to study the cytotoxic activity and IC50 value of aquadest, ethanolic, and chloroform extracts of S. plicata’s pseudobulbs, leaves, and whole plants on T47D cells (breast cancer cells line) as well as cytotoxic activity of the s

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Hussain, Khalid, Zhari Ismail, Amirin Sadikun, Pazilah Ibrahim, and Amin Malik. "IN VITRO ANTIANGIOGENESIS ACTIVITY OF STANDARDIZED EXTRACTS OF Piper sarmentosum Roxb." Jurnal Riset Kimia 1, no. 2 (2015): 146. http://dx.doi.org/10.25077/jrk.v1i2.50.

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ABSTRACT This study was undertaken to investigate the antiangiogenesis activity of standardized extracts/fractions of the leaf of Piper sarmentosum, using rat aorta model. The pulverized leaf was extracted sequentially and methanol extract was further fractionated with hexane, chloroform and ethylacetate. Both extracts and fractions were standardized by reverse phase HPLC with UV detection at 260 nm, using two markers, sarmentine and sarmentosine. Chloroform and methanol extracts have exhibited antiangiogenesis activity of 100% and 20% respectively. Antiangiogenesis activity of hexane and chlo

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Meriö-Talvio, Heidi, Jinze Dou, Tapani Vuorinen, and Leena Pitkänen. "Fast HILIC Method for Separation and Quantification of Non-Volatile Aromatic Compounds and Monosaccharides from Willow (Salix sp.) Bark Extract." Applied Sciences 11, no. 9 (2021): 3808. http://dx.doi.org/10.3390/app11093808.

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Willow bark water extracts contain a mixture of chemically heterogeneous compounds. Fast screening techniques of the extracts are often needed to obtain information on the profile of bioactive and/or other valuable components in the extract. This is, however, a challenging task due to the different chemical structures of the components. Willow bark extract from the hybrid Karin contains several bioactive compounds such as aromatic picein, triandrin, and (+)-catechin. Willow bark extract also contains significant amounts of the monosaccharides fructose and glucose. Here, we demonstrate the appl

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Suwandri, Suwandri, Hartiwi Diastuti, and Purwati Purwati. "ISOLASI DAN IDENTIFIKASI SENYAWA KIMIA SERTA UJI AKTIVITAS ANTICANDIDAISIS SERBUK BATANG SIRIH DUDUK (Piper sarmentosum Roxb. Ex Hunter)." Molekul 2, no. 2 (2007): 53. http://dx.doi.org/10.20884/1.jm.2007.2.2.33.

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Sirih duduk or Piper sarmentosum Roxb. ex Hunter have been long used the people in Indonesia for traditional medicine to cure various diseases. One of them was used to treat fungoid diseases. This research was aimed to examine the anticandidaisis activity from extract of P. sarmentosum bark, isolate and identify anticandidaisis compounds from P. sarmentosum bark. The isolation of bioactive compounds of P. sarmentosum bark was performed by extraction the powder of P. sarmentosum bark using methanol. The methanol extract was then fractionated using n-hexane, chloroform and ethylasetat. The fract

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Ahsan, MQ, MT Alam, MMU Chowdhury, MT Nasim, and SMS Islam. "In Vitro and in Vivo Evaluation Of Pharmacological Activites of Pouzolzia Sanguinea." Journal of Bio-Science 29, no. 2 (2021): 31–42. http://dx.doi.org/10.3329/jbs.v29i2.54952.

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Pouzolzia sanguinea grows in tropical and sub-tropical regions of Bangladesh, used for a variety of purposes including pain, rheumatoid kidney diseases in traditional medicine. The crude ethanolic leaf extract of P. sanguinea with its different fractions (ethanol, n-hexane, and chloroform) was investigated for phytochemical constituents, in vitro antioxidant, anti-inflammatory effects, in-vivo analgesic and antipyretic activities. Preliminary phytochemical constituents were identified by chemical group test. P. sanguinea fractionated extracts contain alkaloids, glycosides, tannins, flavonoids,

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Iwata, T., Y. Morotome, T. Tanabe, M. Fukae, I. Ishikawa, and S. Oida. "Noggin Blocks Osteoinductive Activity of Porcine Enamel Extracts." Journal of Dental Research 81, no. 6 (2002): 387–91. http://dx.doi.org/10.1177/0810387.

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Enamel extracts induce biomineralization such as osteogenesis and cementogenesis, but the molecular component responsible for this activity remains uncertain. We fractionated enamel extracts from developing pig teeth and isolated the osteoinductive fraction. Proteins from pig enamel scrapings were extracted under alkaline conditions (pH 10.8) and fractionated with the use of a Sephadex G-100 (size exclusion) column. The ability of each fraction to enhance alkaline phosphatase (ALP) activity was assayed in ST2 cells, a mouse bone marrow stromal cell line. The osteoinductive fraction of enamel e

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Dimetry, Nadia Zikry, Soad El-Gengaihi, Mahmoud Hafez, and Magdy Hassan Abbass. "Pesticidal activity of certain plant extracts and their isolates against the cowpea beetle Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae: Bruchinae)." Herba Polonica 61, no. 3 (2015): 77–92. http://dx.doi.org/10.1515/hepo-2015-0024.

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SummaryDifferent extracts from seven plant species were assayed against the cowpea beetle (Callosobruchus maculatus) in the laboratory. The plants were extracted sequentially with petroleum ether, chloroform and ethyl alcohol. The petroleum ether extract of each was fractionated into sap and unsap, then identified by GC chromatography. Also compounds isolated from chloroform and alcohol extracts ofCitrullus colocynthisand petroleum ether extract ofNicandra physaloideswere tested for their toxicity against the adult beetle. The sensitivity of the adults to various crude extracts revealed great

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da Silva, Leomara Andrade, Maria Teresa Fachin-Espinar, David Ribeiro da Silva, Nadia Cristina Falcao-Bücker, and Cecilia Veronica Nunez. "Phytochemical study and biological evaluation of Campsiandra comosa hexane and methanol extracts." Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas 24, no. 4 (2025): 585–99. https://doi.org/10.37360/blacpma.25.24.4.41.

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Campsiandra comosa (Fabaceae) is a medicinal Amazonian plant with no previous chemical fractionation. Thus, the objective of the current study was to evaluate extract toxicity on Artemia salina, antioxidant activity (DPPH and Fe3+/Phenanthroline), and antibacterial and antiangiogenic activity of C. comosa, and to perform a chemical fractionation. Leaf hexane and trunk bark methanolic extracts, after phytochemical prospection, were fractionated. Isolated substances were identified by nuclear magnetic resonance (NMR) (1D and 2D)and mass spectrometry (MS). Chromatographic fractionation allowed th

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Ismayati, Maya, Deni Zulfiana, Setiawan Khoirul Himmi, et al. "Antimicrobial Activity of Ten Extractives from Toba, North Sumatra and Mt. Merapi National Park Regions, Indonesia." Jurnal Sylva Lestari 9, no. 1 (2021): 76. http://dx.doi.org/10.23960/jsl1976-85.

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Investigating beneficial chemical compounds of plant extracts is one of the ways to prevent biodiversity loss. This study evaluated the antimicrobial activity of indigenous plant extracts from Toba, North Sumatra, and Mt. Merapi National Park regions against Escherichia coli, Bacillus subtilis, Salmonella typhi, Staphylococcus aureus, Candida albicans, and Candida tropicalis by calculating the zone of microbial growth inhibition. Among the plant extracts, T5 that identified asToona sinensisshowed the highestmicrobial inhibitionto the growth of C. albicans, B. subtilis, S. typhi, and E. coli wi

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Garga, M. A., M. K. Ibrahim, A. Mohammad, et al. "IN VITRO ANTIMALARIAL ACTIVITY OF LEAVE PETROLEUM ETHER EXTRACT OF FICUS SYCOMORUS PLANT." International Journal of Research -GRANTHAALAYAH 8, no. 12 (2021): 279–83. http://dx.doi.org/10.29121/granthaalayah.v8.i12.2020.2677.

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In vitro anti-malarial activity of petroleum ether extracts of Ficus sycomorus leaves was investigated using Candle Jar method. The extractions were carried out through activity guided fractionation. Petroleum ether extract has the highest activity, at the concentration of 1mg and 0.5mg with percentage growth inhibition of (33.12 and 31.9%) respectively. These results suggest that the leaves of Ficus sycomorus plant have significant antimalarial activity and that the antiplasmodial agents might be as a result of the extract fractionated using the petroleum.

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Garga, M. A., M. K. Ibrahim, A. Mohammad, et al. "IN VITRO ANTIMALARIAL ACTIVITY OF LEAVE PETROLEUM ETHER EXTRACT OF FICUS SYCOMORUS PLANT." International Journal of Research -GRANTHAALAYAH 8, no. 12 (2021): 279–83. http://dx.doi.org/10.29121/granthaalayah.v8.i12.2020.2677.

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In vitro anti-malarial activity of petroleum ether extracts of Ficus sycomorus leaves was investigated using Candle Jar method. The extractions were carried out through activity guided fractionation. Petroleum ether extract has the highest activity, at the concentration of 1mg and 0.5mg with percentage growth inhibition of (33.12 and 31.9%) respectively. These results suggest that the leaves of Ficus sycomorus plant have significant antimalarial activity and that the antiplasmodial agents might be as a result of the extract fractionated using the petroleum.

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Safitri, Dewi, Elin Yulinah Sukandar, Irda Fidrianny, and Eriwan Susanto. "THE STUDY OF ANTIFUNGAL ACTIVITY FROM INDIGENOUS PLANTS FROM INDONESIA: AN IN VITRO STUDY." Asian Journal of Pharmaceutical and Clinical Research 10, no. 1 (2016): 196. http://dx.doi.org/10.22159/ajpcr.2017.v10i1.14838.

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Objective: This study aimed to look for new agents from extracted Indonesia herbs which have antifungal activity with better safety profile against Candida albicans, Microsporum gypseum, and Trichophyton mentagrophytes. Methods: Screening of eleven herbs was perform to determine the highest antifungal activity. Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) of selected extracts, rosemary (Rosmarinus officinalis) leaves, Java turmeric (Curcuma xanthorriza) rhizome, and ginger (Zingiber officinalis) rhizome; were determined by using microdilution and agar diffu

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Pham, Uyen To, Y. Thi Nhu Hoang, and Bao Le. "Evaluating antibacterial efficacy of extracts from Ludwigia hyssopifolia against pathogenic bacteria." HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY 14, no. 2 (2024): 39–47. http://dx.doi.org/10.46223/hcmcoujs.tech.en.14.2.3014.2024.

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Ludwigia hyssopifolia extracts have historically been consumed as a healthful drink and rich in biomass. Therefore, the aims of this study were to determine the most effective fractionated extracts of L. hyssopifolia against pathogens. The whole plants of L. hyssopifolia were extracted various fractions with methanol, hexane, chloroform, ethyl acetate, and aqueous. The total phenolics and flavonoids was screened using quantitative approaches. The extracts tested for antibacterial activity against pathogens, including Bacillus cereus, Staphylococcus aureus, Escherichia coli, Salmonella Typhi, a

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Reddy, V. V. Ravichandra, P. Radhika, R. Chidananda Swamy, and D. Raju. "Isolation and Characterization of Anticancer Properties of Compounds from the Leaf Extracts of Vitex negundo." Asian Journal of Chemistry 37, no. 2 (2025): 439–46. https://doi.org/10.14233/ajchem.2025.33147.

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Vitex negundo belongs to the family of Lamiaceae and is popularly known as sambhaloo or Chinese chaste tree. It is widely used as Indian medicinal shrub to prevent a variety of ailments including cancer. The aim of the study was to investigate the anticancer activity of solvent extracts and fractionates of Vitex negundo (VN) leaves against human lung carcinoma epithelial cells A549 by MTT assay. The leaves were extracted with various solvents by ultrasonic-assisted extraction method, in the sequel of non-polar to polar solvents from n-hexane, chloroform, ethyl acetate and ethanol:water (1:1).

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Dojnov, Biljana, N. Loncar, Natasa Bozic, Vera Nenadovic, Jelisaveta Ivanovic та Z. Vujcic. "Comparison of α-amylase isoforms from the midgut of Cerambyx cerdo L. (Coleoptera: Cerambycidae) larvae developed in the wild and on an artificial diet". Archives of Biological Sciences 62, № 3 (2010): 575–84. http://dx.doi.org/10.2298/abs1003575d.

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?-Amylase isoforms of Cerambyx cerdo larvae from the wild (ML and SL) and reared in the laboratory (ADL) were compared. Three amylase isoforms were presented in the SL and ML extracts while two isoforms were presented in the ADL according to zymogram after isoelectric focusing (IEF). All C. cerdo amylase isoforms were acidic proteins (pI < 3.5). Seven amylase isoforms (ACC 1-7) from the midgut of C. cerdo larvae were found in the ML midgut extract, six in the SL extract, and four in the ADL extract according to native PAGE zymogram. The ADL amylase had the highest activity. All crude midgut

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Baskar, Kathirvelu, Chelliah Muthu, and Savarimuthu Ignacimuthu. "Ovicidal Activity ofCouroupita guianensis(Aubl.) againstSpodoptera litura(Fab.)." Psyche: A Journal of Entomology 2014 (2014): 1–5. http://dx.doi.org/10.1155/2014/783803.

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Hexane, chloroform, and ethyl acetate extracts ofCouroupita guianensisleaves were studied for ovicidal activity againstS. litura. All the extracts showed ovicidal activity againstS. litura. Maximum activity was noticed in hexane extract and it showed the least LC50and LC90values; the regression equation was also higher than the other extracts. All the analyzed values showed homogeneity variance. The active hexane extract was fractionated and eight fractions were isolated. The fractions were studied at different concentrations. Among the fractions, fraction 8 showed maximum ovicidal activity wi

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Sanches, Neviton Rogério, Diógenes Aparício Garcia Cortez, Michelle Simone Schiavini, Celso Vataru Nakamura, and Benedito Prado Dias Filho. "An evaluation of antibacterial activities of Psidium guajava (L.)." Brazilian Archives of Biology and Technology 48, no. 3 (2005): 429–36. http://dx.doi.org/10.1590/s1516-89132005000300014.

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The present study was designated to evaluate the antibacterial activities of aqueous and ethanol:water extracts from leaves, roots and stem bark of Psidium guajava L. The antibacterial activities of the extracts against bacteria were tested by using both microdilution assay. The aqueous extracts of P. guajava leaves, roots and stem bark were active against the gram-positive bacteria Staphylococcus aureus (MICs=500, 125 and 250 µg/ml, respectively) and Bacillus subtilis (MICs=500 µg/ml), and virtually inactive against the gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa (MICs

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Lay, Ma Ma, Saiful Anuar Karsani, Behrooz Banisalam, Sadegh Mohajer, and Sri Nurestri Abd Malek. "Antioxidants, Phytochemicals, and Cytotoxicity Studies onPhaleria macrocarpa(Scheff.) Boerl Seeds." BioMed Research International 2014 (2014): 1–13. http://dx.doi.org/10.1155/2014/410184.

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In recent years, the utilization of certain medicinal plants as therapeutic agents has drastically increased.Phaleria macrocarpa(Scheff.) Boerl is frequently used in traditional medicine. The present investigation was undertaken with the purpose of developing pharmacopoeial standards for this species. Nutritional values such as ash, fiber, protein, fat, and carbohydrate contents were investigated, and phytochemical screenings with different reagents showed the presence of flavonoids, glycosides, saponin glycosides, phenolic compounds, steroids, tannins, and terpenoids. Our results also reveale

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Al-abri, Faisal Said Hamed, Salem Said Jaroof Al Touby, and Mohammad Amzad Hossain. "A comparative study of the antioxidant, antibacterial, and cytotoxic activities of different varieties of imported ripe Cavendish banana." International Journal of Secondary Metabolite 11, no. 4 (2024): 623–32. https://doi.org/10.21448/ijsm.1338699.

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Traditionally, Omanis used bananas to treat gastrointestinal disorders, constipation, and diarrhea. The study aims to determine the pharmacological and toxicological activities of two imported banana varieties collected from the local fruit market. Extracts were prepared separately by soaking the bananas in methanol for seventy-two hours. Then, the extracts were fractionated with various solvents with increasing patterns of polarity to give corresponding crude extracts. All extracts were used to determine their antioxidant, antibacterial, and cytotoxic activities using 2,2-diphenyl-1-picrylhyd

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Oke-Altuntas, Feyza, Mehmet Ali Demirci, Ibrahim Demirtas, Ayse Sahin Yaglioglu, and Lutfi Behcet. "Phytochemical Screening, Antiproliferative and Antioxidant Properties of Various Extracts from Endemic Origanum acutidens." Combinatorial Chemistry & High Throughput Screening 21, no. 4 (2018): 281–91. http://dx.doi.org/10.2174/1386207321666180416154404.

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Aim and Objective: Origanum acutidens (Hand.–Mazz.) Ietsw. is an endemic and perennial plant grown mainly in East Anatolia. Recently, natural plant products have attracted interest due to their safety and therapeutic effects. Therefore, the aim of this study was to investigate phytochemical contents and biological effects of Origanum acutidens. Materials and Methods: The aerial parts of O. acutidens were extracted with water, ethyl acetate, nbutanol, and methanol/chloroform solvents. Phenolic compounds and other constituents of the extracts were analyzed by HPLC/TOF-MS. The Ethyl Acetate extra

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Márquez-Rodríguez, Ana Selene, Susana Nevárez-Baca, Julio César Lerma-Hernández, et al. "In Vitro Antibacterial Activity of Hibiscus sabdariffa L. Phenolic Extract and Its In Situ Application on Shelf-Life of Beef Meat." Foods 9, no. 8 (2020): 1080. http://dx.doi.org/10.3390/foods9081080.

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Compounds from spices and herbs extracts are being explored as natural antibacterial additives. A plant extract used in traditional folk medicine is Hibiscus sabdariffa L., also known as Roselle. Therefore, the potential use of a phenolic hibiscus extract as antibacterial or natural food preservative was analyzed in vitro and in situ. A phenolic extract was obtained from hibiscus calyces and fractionated, and then the fractions were tested against foodborne pathogen bacteria. Liquid–liquid extraction and solid-phase extraction were used to fractionate the hibiscus extract, and HPLC was employe

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Shabrina, Riza, Berna Elya, and Arikadia Noviani. "ANTIOXIDANT ACTIVITIES OF FRACTIONS FROM ETHYL ACETATE EXTRACTS OF GARCINIA FRUTICOSA LAUTERB. LEAVES." International Journal of Applied Pharmaceutics 10, no. 1 (2018): 44. http://dx.doi.org/10.22159/ijap.2018.v10s1.10.

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Objective: This study aimed to fractionate the antioxidant activity of the ethyl acetate leaf extract and to characterize the most active fractionsaccording to compound groups.Methods: The ethyl acetate extract was fractionated with column chromatography using a gradient elution system. Fractions were first screenedqualitatively for antioxidant activity before active fractions were quantified with respect to in vitro antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and the ferric-reducing antioxidant power (FRAP) assay. The compound groups were ident

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Kinjal H, Dr Shah. "Evaluation of Antioxidant Activity of Balanites Roxburghii Planch. Fruit Extracts." International Journal of Scientific Research and Management (IJSRM) 7, no. 12 (2019): 295–304. http://dx.doi.org/10.18535/ijsrm/v7i12.mp03.

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Antioxidant potency of fruits of Balanites roxburghii Planch.crude methanol and its fractionated extracts (hexane, ethyl acetate, methanol and water) have been investigated, employing three different established in vitro testing systems, such as scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, reducing power assay and β-carotene method. The methanol extract of fruits of Balanites roxburghii Planch.showed the best DPPH scavenging activity with the lowest EC 50 34 μg/ml, followed by the ethyl acetate (EC 50 39 μg/ml), hexane (EC 50 48 μg/ml) and water extract (EC 50 54 μg/ml

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IMRAN, Sehrish, Yamin BIBI, Abdul QAYYUM, et al. "Biological and phytochemical analysis of fractionated extracts of Ziziphus nummularia against pathogenic bacteria, oxidative stress and cytotoxicity." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 52, no. 3 (2024): 13663. http://dx.doi.org/10.15835/nbha52313663.

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In modern medicine, synthetic drugs are predominant, yet they often pose health hazards. So, there is a need to find natural alternatives. Ziziphus nummularia (Burm. F.) Wight & Arn., belongs to family Rhamnaceae, is well known for its traditional value such as its fruits have been used as natural appetizer and orally consumed around the world. This study investigated the phytochemical composition and bioactivity of crude and fractionated extracts from the stem bark of Ziziphus nummularia. Crude extracts were prepared using cold maceration, followed by partitioning based on polarity into n

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Öztürk, Mehmet, Ufuk Kolak, Mehmet Emin Duru, and Mansur Harmandar. "GC-MS Analysis of the Antioxidant Active Fractions of Micromeria juliana with Anticholinesterase Activity." Natural Product Communications 4, no. 9 (2009): 1934578X0900400. http://dx.doi.org/10.1177/1934578x0900400923.

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The aerial parts of Micromeria juliana (L.) Bentham ex Reichb. were extracted with light petroleum, acetone and methanol, successively. The antioxidant activity of different concentrations of the extracts was evaluated using different antioxidant tests, namely total antioxidant (lipid peroxidation inhibition activity), DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging, ferric reducing power, and metal chelating. Total antioxidant activity was determined using the β-carotene-linoleic acid assay. Unexpectedly, the light petroleum extract exhibited strong lipid peroxidation inhibition activ

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Santos, Klebson, Andriele Barbosa, Victor Freitas, et al. "Antiproliferative Activity of Neem Leaf Extracts Obtained by a Sequential Pressurized Liquid Extraction." Pharmaceuticals 11, no. 3 (2018): 76. http://dx.doi.org/10.3390/ph11030076.

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Azadirachta indica A. Juss (neem) extracts have been used in pharmaceutical applications as antitumor agents, due to their terpenes and phenolic compounds. To obtain extracts from neem leaves with potential antiproliferative effect, a sequential process of pressurized liquid extraction was carried out in a fixed bed extractor at 25 °C and 100 bar, using hexane (SH), ethyl acetate (SEA), and ethanol (SE) as solvents. Extractions using only ethanol (EE) was also conducted to compare the characteristics of the fractionated extracts. The results obtained by liquid chromatography-electrospray ioniz

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Srisupap, Sirinada, and Chanya Chaicharoenpong. "In vitro antioxidant and antityrosinase activities of Manilkara kauki." Acta Pharmaceutica 71, no. 1 (2021): 153–62. http://dx.doi.org/10.2478/acph-2021-0009.

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AbstractManilkara kauki L. Dubard is a tropical plant in the genus Manilkara of family Sapotaceae. This study investigated the total phenolic and flavonoid contents, and antioxidant and antityrosinase activities in different parts of M. kauki (fruits, leaves, seeds, stem barks and woods) and in fractions of stem barks. The total phenolic and flavonoid contents of the methanol and aqueous crude extracts of different parts of M. kauki ranged from 10.87 to 176.56 mg GAE (gallic acid equivalents) per gram of crude extract and 14.33 to 821.67 mg QE (quercetin equivalents) per gram of crude extract,

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Staszowska-Karkut, Monika, Barbara Chilczuk, Małgorzata Materska, Renata Kontek, and Beata Marciniak. "Phenolic Compounds in Fractionated Blackcurrant Leaf Extracts in Relation to the Biological Activity of the Extracts." Molecules 28, no. 22 (2023): 7459. http://dx.doi.org/10.3390/molecules28227459.

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The aim of this study was to determine the relationship between antioxidant and anticancer properties of extracts from blackcurrant (Ribes nigrum L.) leaves and their fractions and chemical contents. Dried ethanolic extract was divided into three fractions using solid phase extraction: aqueous (F1), 40% MeOH (F2), and 70% MeOH (F3). Both the extract and the fractions were analyzed in terms of antiradical activity (DPPH• and ABTS+•), total phenolic compounds, and total flavonoids. The antitumor potential of the fractions was evaluated in vitro on human colorectal (HCT 116) and prostate (PC-3) c

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Kaur, Virender, Kumud Upadhyaya, and Milind Pande. "BIOASSAY-GUIDED EVALUATION OF FICUS SEMICORDATA FOR ANTIDIABETIC ACTIVITY." International Journal of Pharmacy and Pharmaceutical Sciences 9, no. 3 (2017): 71. http://dx.doi.org/10.22159/ijpps.2017v9i3.16441.

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Objective: The early stage of diabetes mellitus type 2 is associated with postprandial hyperglycemia. The therapeutic approach involved in the treatment of type 2 diabetes mellitus is the use of agents that can decrease postprandial hyperglycemia by inhibiting carbohydrate digesting enzymes. In an effort of identifying herbal drugs which may become useful in the prevention or mitigation of diabetes, the antidiabetic activity of Ficus semicordata (FS) and its constituents were studied. The present study was undertaken in part to identify the potent antihyperglycemic fraction from the ethanol ex

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Budiari, Setyani, Ekowati Chasanah, Maggy Thenawidjaja Suhartono, and Nurheni Sri Palupi. "ANGIOTENSIN CONVERTING ENZYME (ACE) INHIBITORY ACTIVITY OF CRUDE AND FRACTIONATED SNAKEHEAD (Channa striata) MEAT EXTRACT." Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology 13, no. 2 (2018): 57. http://dx.doi.org/10.15578/squalen.v13i2.345.

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The existence of endogenous bioactive protein or peptide with angiotensin-converting enzyme (ACE) inhibitory activity in snakehead meat is promising to be investigated. The purposes of this research were to extract ACE inhibitory endogenous bioactive protein or peptide from snakehead meat and to fractionate the active compounds using ultrafiltration. The extraction in this research employs two kinds of solvents, e.i aquadest and 50% ethanol. Then, the extract is fractionated with the ultrafiltration using the 10,000; 5,000 and 3,000 MWCO membranes to separate the protein or peptide of with the

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