Relevant bibliographies by topics / Fragment restriction

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
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Academic literature on the topic 'Fragment restriction'

Author: Grafiati
Published: 4 June 2021
Last updated: 9 February 2022

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Journal articles on the topic "Fragment restriction"

1

WU, Z., I. NAGANO, E. POZIO, and Y. TAKAHASHI. "Polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) for the identification of Trichinella isolates." Parasitology 118, no. 2 (February 1999): 211–18. http://dx.doi.org/10.1017/s0031182098003679.

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In the present study, polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) analysis was developed to identify 5 species (Trichinella spiralis, Trichinella britovi, Trichinella nativa, Trichinella nelsoni and Trichinella pseudospiralis) and 3 phenotypes of uncertain taxonomic status (Trichinella T5, T6, and T8). Eleven restriction endonucleases were used to restrict 3 DNA fragments (1) a 2800 bp fragment of the 43 kDa excretory–secretory (E–S) protein gene, (2) a 1250 bp fragment amplified with the primer pair SB147A and (3) a 372 bp fragment amplified with the primer pair SB372A. This RFLP method allows the identification of the 8 Trichinella phenotypes as follows: T. spiralis by the HinfI or DdeI endonuclease restriction of the 2800 bp fragment; T. nativa by the RsaI restriction of the 2800 bp fragment, or by the AluI restriction of the 1250 bp fragment; T. britovi and Trichinella T8 by the AluI restriction of the 1250 bp fragments, and can be discriminated between them by the SspI restriction of the 2800 bp fragment; T. pseudospiralis by the MspI restriction of the 372 bp fragment; T. nelsoni by the HhaI or AluI restriction of the 2800 bp fragment; Trichinella T5 by the HhaI restriction of the 2800 bp fragment; Trichinella T6 by the AluI restriction of the 1250 bp fragment; and Trichinella T8 by the SspI or RsaI restriction of the 2800 bp fragment. This study reveals also an intraspecifies polymorphism in the 2800 bp and 1250 bp fragments for T. britovi, Trichinella T5 and T6.
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Hall, Lucinda M. C., and Brigid Duke. "Conservation of Restriction Sites in Isolates ofStreptococcus pneumoniae with Diverse Restriction Fragment Patterns." Journal of Clinical Microbiology 36, no. 6 (1998): 1805–7. http://dx.doi.org/10.1128/jcm.36.6.1805-1807.1998.

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Separation of large restriction fragments by pulsed-field gel electrophoresis is a commonly used method for epidemiological typing ofStreptococcus pneumoniae and many other bacterial species. Information on the genetic changes underlying the restriction fragment polymorphisms that allow discrimination between isolates is scarce. In this study fragments adjacent to ApaI sites in a clinical isolate of S. pneumoniae were cloned and used to probeHindIII and HindIII-plus-ApaI genomic DNA digests from other isolates with very differentApaI fragment patterns. If for a given isolate theHindIII fragment detected by the probe was reduced in size on digestion with ApaI, it was deduced that theApaI site was conserved in that isolate. The results demonstrate that of six ApaI sites in PN93/908 examined, five were retained in 11 genetically different isolates and one was retained in 2 isolates but lost in 9 others. It was concluded that point mutations at restriction sites are unlikely to account for the restriction fragment length polymorphism observed and that much of the polymorphism may be due to DNA rearrangements, possibly resulting from the insertion or deletion of mobile DNA elements.
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Bobola, Michael S., Robert T. Eckert, and Anita S. Klein. "Restriction fragment variation in the nuclear ribosomal DNA repeat unit within and between Picearubens and Piceamariana." Canadian Journal of Forest Research 22, no. 2 (February 1, 1992): 255–63. http://dx.doi.org/10.1139/x92-033.

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The frequencies of polymorphic restriction fragments for the nuclear ribosomal DNA repeat were compared for 12 provenances of red spruce (Picearubens Sarg.) and 34 provenances of black spruce (Piceamariana (Mill.) B.S.P.). Within an individual as many as five distinct ribosomal DNA repeat unit types could be distinguished. Canonical correlation analysis revealed significant variation of restriction fragment frequencies with a geographic variate comprising latitude and longitude of provenances. Geographic origins accounted for 24.7% of the variation in polymorphic restriction fragments in black spruce and 31.8% of the variation in polymorphic restriction fragments in red spruce. Discriminant analysis, using the restriction fragment frequencies for the ribosomal DNA, was used to develop a classification model for the two species. Tenfold verification of the model produced an average correct classification of 99% for black spruce and 96% for red spruce. Plots of canonical scores for the first and second canonical variâtes clearly separated red spruce from black spruce. This study presents a novel combination of restriction fragment frequency data and multivariate analysis to distinguish species that may not always be differentiated using morphological traits.
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Moore, Patrick P. "CHLOROPLAST RESTRICTION FRAGMENT VARIABILITY IN RASPBERRY." HortScience 25, no. 9 (September 1990): 1159f—1159. http://dx.doi.org/10.21273/hortsci.25.9.1159f.

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Cultivated raspberries may include North American red raspberry (Rubus idaeus strigosus Michx), European red raspberry (R. idaeus vulgatus Arrhen.) or black raspberry (R. occidentalis in their pedigrees. Twenty-one raspberry clones were investigated using chloroplast restriction fragment length polymorphisms to determine the cytoplasm type and the amount of cytoplasmic diversity among these selected clones. The raspberry clones were selected representing North American red raspberry, European red raspberry, black raspberry and cultivars with divergent maternal lineages. Total cellular DNA was probed with two 32P-labelled fragments of tomato chloroplast DNA. Probe-restriction enzyme combinations were selected which discriminated between representatives of the two red raspberry subspecies. Raspberry clones were grouped according to the chloroplast restriction fragment patterns. The composition of the groups was compared with their pedigrees.
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Fani, Renato, Marco Bazzicalupo, Enzo Gallori, Luciana Giovannetti, Stefano Ventura, and Mario Polsinelli. "Restriction fragment length polymorphism ofAzospirillumstrains." FEMS Microbiology Letters 83, no. 2 (October 1991): 225–29. http://dx.doi.org/10.1111/j.1574-6968.1991.tb04444.x-i1.

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Williams, Robert C. "Restriction fragment length polymorphism (RFLP)." American Journal of Physical Anthropology 32, S10 (1989): 159–84. http://dx.doi.org/10.1002/ajpa.1330320508.

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Jarcho, John. "Restriction Fragment Length Polymorphism Analysis." Current Protocols in Human Genetics 1, no. 1 (May 1994): 2.7.1–2.7.15. http://dx.doi.org/10.1002/0471142905.hg0207s01.

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Chowdhury, SMZH, AR Omar, A. Ideris, H. Bejo, and AA Jamaluddin. "Restriction endonuclease analysis of the genomes of different isolates of chicken anemia virus amplified by polymerase chain reaction." SAARC Journal of Agriculture 15, no. 2 (January 25, 2018): 1–18. http://dx.doi.org/10.3329/sja.v15i2.35155.

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Four DNA fragments (fragments A, B, C and D) covering the whole genome of chicken anemia virus (CAV) were amplified enzymatically by polymerase chain reaction (PCR) using four pairs of oligonucleotide primers. The DNA fragments were amplified from each of nine CAV isolates including eight Malaysian isolates and one European isolate (Cux-1). For all nine CAV isolates, fragment A (1518 bp) was digested with one restriction enzyme, Eco130I (StyI); fragment B (926 bp) with three enzymes, Eco130I (StyI), HpaII and MboI separately; fragment C (675 bp) with also three enzymes, BsuRI (HaeIII), HinfI, and HpaII separately; and the fragment D (552 bp) with one enzyme, EcoRI. Enzyme digested products of different fragments were separated by agarose gel or polyacrylamide gel electrophoresis. Each of the eightenzymatic reactions differentiated at least two isolates except the HpaII digestion of fragment C where no isolate was distinguished. The overall restriction endonuclease (RE) analysis separated four isolates (BL-1, BL- 2, BL-4 and BL-5) in one group and the rest five isolates (SMSC-1, SMSC-2, 3-1, BL-3 and Cux-1) were differentiated from each other and also from the group of four isolates, based on the number of restriction site differences and the fragments generated by different enzymatic digestions. The study revealed that RE analysis could be used to identify and differentiate CAV isolates based on the number of restriction site differences. The study showed that more isolates, even the isolates from the same poultry farm, could be differentiated with proper genomic diversity after RE analysis of more genome fragments compared to that of single genome fragment.SAARC J. Agri., 15(2): 1-18 (2017)
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Curran, J., D. L. Baillie, and J. M. Webster. "Use of genomic DNA restriction fragment length differences to identify nematode species." Parasitology 90, no. 1 (February 1985): 137–44. http://dx.doi.org/10.1017/s0031182000049088.

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Restriction endonuclease digestion of genomic DNA generates DNA fragments of unique size, dependent upon the particular base sequence. Following fractionation by agarose gel electrophoresis, repetitive DNA can be visualized as distinct bands in stained gels and the restriction fragment length of such bands used as diagnostic characters. Restriction fragment length differences were detected between species within the genera Trichinella, Caenorhabditis, Romanomermis, Steinernema (syn. Neoaplectana) and Meloidogyne. This technique provides a new tool for the taxonomist, which is independent of phenotypic variation and it enables the rapid and reliable separation of closely related species.
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Traut, W. "Hypervariable Bkm DNA Loci in a Moth, Ephestia kuehniella : Does Transposition Cause Restriction Fragment Length Polymorphism?" Genetics 115, no. 3 (March 1, 1987): 493–98. http://dx.doi.org/10.1093/genetics/115.3.493.

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ABSTRACT Bkm sequences, originally isolated from snake satellite DNA, are a component of eukaryote genomes with a preferential location on sex chromosomes. In the Ephestia genome, owing to the presence of only a few Bkm-positive BamHI restriction fragments and to extensive restriction fragment length polymorphisms between and within inbred strains, a genetic crossbreeding analysis was feasible. No sex linkage of Bkm was detected. Instead-depending on the strain-two or three autosomal Bkm DNA loci were identified. All three loci were located on different chromosomes. Fragment length and transmission of fragments was stable in some crosses. In others, changes in fragment length or loss of the Bkm component were observed, probably depending on the source strain of the fragment. The anomalous genetic behaviour is best accounted for by the assumption that Bkm sequences are included in mobile genetic elements.
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Dissertations / Theses on the topic "Fragment restriction"

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Liu, Ni. "Detection of trait-associated restriction fragment length polymorphisms in chicken." Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=55509.

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The gene encoding chicken growth hormone (GH) was isolated from a chicken genomic library. The size of the gene was 4 kb. It was digested with PstI and subcloned into pUC18. Three of the PstI fragments were used for restriction fragment length polymorphisms (RFLPs) analysis at the GH locus in two chicken strains (fat and lean line). Four polymorphic sites were detected using a PstI fragment (PII) as a probe. One polymorphism was located at a SacI restriction site (PS1), and three at MspI sites (PM1, PM2 and PM3). A method based on polymerase chain reaction (PCR) was developed for detecting polymorphisms at PM3 site. A fragment of 823 base pairs which contained the PM3 polymorphic site was amplified. Three genotypes (+/+,$-$/$-$ and +/$-$) were distinguished by examining the MspI digested PCR products in either agarose or polyacrylamide gel.
Ten anonymous cDNA clones were also isolated from a chicken liver cDNA library and used for RFLPs analysis. Three of these clones were found to be able to detected RFLPs at MspI sites in chicken strains (strain 7, 8, 9, 8R, S and K) indicating that a high frequency of genes are polymorphic and can be used as markers in mapping experiments. One of the three clones was present on a haploid genetic element. Segregation analysis showed that the inheritance of this haploid gene was determined by the genotype of the female parent.
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Chui, Hon-kit. "Restriction fragment length polymorphism analysis of a hospital outbreak of tuberculosis." Click to view the E-thesis via HKUTO, 2001. http://sunzi.lib.hku.hk/hkuto/record/B31970266.

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Chui, Hon-kit, and 徐漢傑. "Restriction fragment length polymorphism analysis of a hospital outbreak of tuberculosis." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B31970266.

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Cicek, Mine II. "Genetic Analysis of Quantitative Trait Loci Associated with Seed Sucrose Content Using Molecular Markers in an Interspecific Glycine Cross." Thesis, Virginia Tech, 1997. http://hdl.handle.net/10919/36506.

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Sucrose content is one of the important seed quality traits in soybean, especially for oriental soyfood production. However, little genetic information is available on this quantitative trait yet. A previous study was conducted on seed sucrose content of soybean using a population of F2-derived lines from an interspecific cross between an adapted high-sucrose (8.3%) G. max breeding line (V71-370) and a low sucrose (1.6%) G. soja plant introduction (PI407162). Nineteen marker loci, mapping to seven linkage groups (A1, A2, E, F, L1, I, and M), were significantly associated with seed sucrose content after screening 178 polymorphic genetic markers, including RFLPs, SSRs, RAPDs and morphological markers. The replicated field experiments were planted in 1993 and 1995. The objective of my study was to evaluate QTLs associated with seed sucrose content utilizing an additional 153 F2:3 families from the same cross. DNA samples from the additional families were analyzed with the nineteen genetic markers associated with sucrose in the previous study. Sucrose data were obtained from seeds harvested from a field experiment conducted in 1995. Single factor analysis of variance results for the sucrose data obtained from the 153 F2:3 families were compared to the 1995 data for the 144 F2:3 families of the previous study. Of the nineteen genetic markers significantly associated with seed sucrose content in the previous study, seven were also significantly associated in this study. These genetic markers include sgA458a on linkage group A2, NBS61 on linkage group E, sgB164, R-B4a and sgB162 on linkage group L1, and R-B4b and sgA144 on linkage group I. The percent phenotypic variation explained by significant individual markers varied from 2.9 to 6.8% in the 153 F2:3 families. This study shows that seed sucrose content, a quantitative trait, may be improved using the molecular marker technology. Further research is necessary in different genetic backgrounds of G. max in order to implement these markers in a breeding program for selection.
Master of Science
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Gysling, Kevin. "Polymerase chain reaction restriction fragment length polymorphism identification of trebouxia lichen photosymbionts." Honors in the Major Thesis, University of Central Florida, 2009. http://digital.library.ucf.edu/cdm/ref/collection/ETH/id/1269.

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This item is only available in print in the UCF Libraries. If this is your Honors Thesis, you can help us make it available online for use by researchers around the world by following the instructions on the distribution consent form at http://library.ucf.edu/Systems/DigitalInitiatives/DigitalCollections/InternetDistributionConsentAgreementForm.pdf You may also contact the project coordinator, Kerri Bottorff, at kerri.bottorff@ucf.edu for more information.
Bachelors
Medicine
Molecular Biology and Microbiology
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Gray, Stephen James. "The genotyping of Neisseria meningitidis by restriction fragment length polymorphism (RFLP) analysis." Thesis, Manchester Metropolitan University, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360085.

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Stevens, Tracy Alison. "Analysis of mitochondrial DNA restriction fragment patterns in killer whales, Orcinus orca." PDXScholar, 1989. https://pdxscholar.library.pdx.edu/open_access_etds/3928.

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The mitochondrial DNA restriction fragment patterns of killer whales (Orcinus orca) were investigated in order to determine the level of genetic differentiation that exists between killer whales from various geographic locations. Twenty one killer whales were examined, seventeen of which were captive killer whales that originated from the North Atlantic and Northeast Pacific Oceans. Two were captive-born animals and two were killer whales that stranded along the Northeast Pacific coast. DNA was extracted from blood and/or tissue samples, cleaved with a variety of restriction endonucleases and the DNA fragments were separated by horizontal agarose gel electrophoresis. The DNA was then transferred to nylon membranes and the killer whale mitochondrial DNA was visualized by hybridization to the complete mitochondrial DNA genome of Commerson's dolphin (Cephalorhynchus commersonii). The resultant restriction fragment patterns were analyzed to determine whether mitochondrial DNA variation was present between killer whales from different geographic regions or between communities and pods of killer whales from the same geographic location.
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Ogawa, Osamu. "Molecular Genetic Analysis of Human Renal Tumors by Using Restriction Fragment Length Polymorphisms." Kyoto University, 1994. http://hdl.handle.net/2433/160715.

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本文データは平成22年度国立国会図書館の学位論文(博士)のデジタル化実施により作成された画像ファイルを基にpdf変換したものである
Kyoto University (京都大学)
0048
新制・課程博士
博士(医学)
甲第5794号
医博第1587号
新制||医||590(附属図書館)
UT51-94-R318
京都大学大学院医学研究科外科系専攻
(主査)教授 野田 亮, 教授 佐々木 正夫, 教授 吉田 修
学位規則第4条第1項該当
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Zhang, Jianhua. "Restriction fragment length polymorphism analysis of chloroplast DNA, mitochondrial DNA, and ribosomal DNA in turfgrasses." Diss., This resource online, 1994. http://scholar.lib.vt.edu/theses/available/etd-06062008-170748/.

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Guo, Haibin. "Combining Conventional Tests and Terminal Restriction Fragment Analysis to Evaluate Microbial Quality of Raw Milk." DigitalCommons@CalPoly, 2011. https://digitalcommons.calpoly.edu/theses/468.

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p.p1 {margin: 12.0px 0.0px 3.0px 0.0px; text-align: center; font: 16.0px 'Times New Roman'} p.p2 {margin: 0.0px 0.0px 10.0px 0.0px; text-align: center; font: 12.0px 'Times New Roman'} p.p3 {margin: 0.0px 0.0px 10.0px 0.0px; font: 12.0px 'Times New Roman'} Abstract Combining Conventional Tests and Terminal Restriction Fragment Analysis to Evaluate Microbial Quality of Raw Milk Haibin Guo The dairy industry is an important part in the domestic economy in the U.S. and the quality of dairy products hinges on raw milk quality. Microorganisms play a critical role in raw milk quality and they are currently tested and monitored by conventional microbiological tests. Some of the most common conventional tests include somatic cell count (SCC), standard plate count (SPC), coliform count (CC), lab pasteurized count (LPC) and proteolytic strain count (PSC). However, these methods do not correlate with each other or with the quality of milk and milk products. One factor that contributes to this lack of correlation is the insufficient knowledge of microbial communities in raw milk. In this work we aimed to evaluate modern molecular methods to complement traditional quality procedures that may eventually complement conventional tests and improve milk quality evaluation. Therefore, a molecular method, Terminal Restriction Fragment (TRF) analysis was introduced. TRF analysis has been widely used as a tool to investigate the microbial communities in environmental samples. In this study, it was applied to investigate the microbial communities in raw milk and evaluate raw milk quality. Milk samples were collected for over six months in the Cal Poly dairy farm and evaluated by conventional tests and TRF analysis. Samples were defined as “high quality” milk and “low quality” milk according to each conventional test first. The cutoffs applied were: 50,000 cfu/ml for SPC, 70,000 cells/ml for SCC, 100 cfu/ml for CC and 250 cfu/ml for LPC. TRF analysis was conducted on raw milk samples subsequently. DNA extraction was optimized. Non-Parametric Multivariate Analysis of Variance (NPMANOVA) was applied to TRF profiles from low and high quality milk. The analysis of Similarity of Percentage (SIMPER) was used to determine each TRF peak’s contribution to the dissimilarity between the profiles of high and low quality milk. The genus/species represented by TRF peaks were estimated via database matching. In addition, conventional tests and TRF analysis were also used to analyze the factors causing low quality milk. Rain event and cow’s apparent health were the two factors investigated since raw milk samples were collected from cows in different apparent health status on wet days and dry days. Conventional tests revealed strong correlations between the results of SPC and PSC, and SPC and CC (coefficients of correlation > 0.8). It implied that the results of conventional tests might not be independent, so the statistics based on the assumption of independence of variables were not suitable to analyze the data. SCC showed no strong correlation with any other conventional tests. Raw milk samples were grouped as high quality and low quality according to SPC, CC, SCC and LPC. Using TRF analysis, it was found that there was a significant difference between TRF profiles from low and high quality milk when the quality was defined by SPC or LPC. A TRF peak at 268 bp generated by DpnII was predominant in the TRF profiles and had high abundance in the profiles of low quality milk. Hence, Pseudomonas spp. represented by TRF peak at 268 bp was likely the predominant bacteria in the microbial community associated with raw milk. TRF peaks at 61 bp, 81 bp, 104 bp, 104 bp, 201 bp, 242 bp, 268 bp, and 270 bp contributed the most to the dissimilarity between TRF profiles from different groups of samples. In addition, the presence of DNA derived from viable but non-culturable species that were associated with raw milk quality was detected. Rain event was the most important factor affecting the microbial quality of raw milk in this study. Both the conventional tests and TRF analysis showed that there was a significant difference between raw milk samples collected on wet days versus dry days. Samples collected on wet days harbored high bacterial counts and had high abundance of the predominant TRF peaks. In addition, the same TRF peaks contributing the most to the dissimilarity between groups separated by rain event were found to be among those contributing the most to the dissimilarity between groups of high and low quality milk defined by conventional tests. During wet days, the low quality milk was likely caused by the increased dirtiness of cow’s teats. Soil microbes are often associated with microorganisms in raw milk such as psychrotrophic bacteria, coliform groups and spore-formers. Cow’s apparent health status showed no significant influence on the microbial quality of raw milk. Overall, the combination of conventional tests and TRF analysis can yield a comprehensive understanding of microbial community in raw milk and improve the evaluation of raw milk quality. TRF analysis was demonstrated as a useful tool and a complement to conventional tests for milk quality evaluation by providing more information on the microbial community associated with raw milk. Findings in this study can offer a basis for further study and may help the dairy industry improve raw milk quality evaluation system.
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Books on the topic "Fragment restriction"

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Bhattacherjee, Vasker. Variation in the restriction fragment length in the DNA of species of 'Aspergillus' and 'Penicillium'. Birmingham: University of Birmingham, 1989.

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Oelbaum, Raymond Stuart. An analysis of four candidate genes for non-insulin-dependent diabetes using restriction fragment length polymorphism markers. Manchester: University of Manchester, 1994.

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Lefranc, Marie-Paule, and G. Lefranc. Restriction Fragment Length Polymorphism (Pediatrician). S. Karger AG (Switzerland), 1989.

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Liou, Pan-Chi. A molecular study of the citrus genome through restriction fragment length polymorphism and isozyme mapping. 1990.

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Gray, Stephen James. The genotyping of neisseria meningitidis by restriction fragment lengthpolymorphism (RFLP) analysis. 1996.

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Smith, Myron Lloyd *. Mitochondrial DNA restriction fragment length polymorphisms in the North American biological species "Armillaria mellea". 1988.

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Caramello, Olivia. Some Applications. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198758914.003.0012.

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This chapter describes some applications of the theory developed in the previous chapters in a variety of different mathematical contexts. The main methodology used to generate such applications is the ‘bridge technique’ presented in Chapter 2. The discussed topics include restrictions of Morita equivalences to quotients of the two theories involved, give a solution to a prozblem of Lawvere concerning the boundary operator on subtoposes, establish syntax-semantics ‘bridges’ for quotients of theories of presheaf type, present topos-theoretic interpretations and generalizations of Fraïssé’s theorem in model theory on countably categorical theories and of topological Galois theory, develop a notion of maximal spectrum of a commutative ring with unit and investigate compactness conditions for geometric theories allowing one to identify theories lying in smaller fragments of geometric logic.
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Convention on Prohibitions or Restrictions on the Use of Certain Conventional Weapons: Message from the President of the United States transmitting the convention on prohibitions or restrictions on the use of certain conventional weapons which may be deemed to be excessively injurious or to have indiscriminate effects, and two accompanying protocols on non-detectable fragments (Protocol I) and on prohibitions or restrictions on the use of mines, booby-traps and other devices (Protocol II). Washington: U.S. G.P.O., 1994.

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Convention on Prohibitions or Restrictions on the Use of Certain Conventional Weapons: Message from the President of the United States transmitting the Convention on Prohibitions or Restrictions on the Use of Certain Conventional Weapons Which May Be Deemed To Be Excessively Injurious or To Have Indiscriminate Effects, and two accompanying protocols on non-detectable fragments (Protocol I) and on prohibitions or restrictions on the use of mines, booby-traps and other devices (Protocol II). Washington: U.S. G.P.O., 1995.

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Book chapters on the topic "Fragment restriction"

1

Rolfs, Arndt, Irmela Schuller, Ulrich Finckh, and Ines Weber-Rolfs. "Restriction Fragment Analysis." In PCR: Clinical Diagnostics and Research, 136–42. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-77492-8_11.

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Bernatzky, Robert. "Restriction fragment length polymorphism." In Plant Molecular Biology Manual, 383–400. Dordrecht: Springer Netherlands, 1988. http://dx.doi.org/10.1007/978-94-017-5294-7_18.

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Chaudhary, Reema, and Ganesh Kumar Maurya. "Restriction Fragment Length Polymorphism." In Encyclopedia of Animal Cognition and Behavior, 1–3. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-319-47829-6_175-1.

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Green, Elaine K. "Restriction Fragment-Length Polymorphisms." In Springer Protocols Handbooks, 271–79. Totowa, NJ: Humana Press, 1998. http://dx.doi.org/10.1007/978-1-59259-642-3_22.

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Bernatzky, Robert. "Restriction fragment length polymorphism." In Plant Molecular Biology Manual, 467–84. Dordrecht: Springer Netherlands, 1989. http://dx.doi.org/10.1007/978-94-009-0951-9_24.

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Potter, Robert H. "Restriction fragment analysis of somaclones." In Plant Tissue Culture Manual, 517–33. Dordrecht: Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-009-0103-2_29.

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Gooch, Jan W. "Restriction Fragment Length Polymophism (RFLP)." In Encyclopedic Dictionary of Polymers, 921. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_14694.

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Leppla, Norman C., Bastiaan M. Drees, Allan T. Showler, John L. Capinera, Jorge E. Peña, Catharine M. Mannion, F. William Howard, et al. "Restriction Fragment Length Polymorphism (RFLP)." In Encyclopedia of Entomology, 3174. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6359-6_3373.

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Nelson, William, and Carol Soderlund. "Software for Restriction Fragment Physical Maps." In The Handbook of Plant Genome Mapping, 285–305. Weinheim, FRG: Wiley-VCH Verlag GmbH & Co. KGaA, 2005. http://dx.doi.org/10.1002/3527603514.ch12.

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Demezas, David H. "Fingerprinting Bacterial Genomes using Restriction Fragment Length Polymorphisms." In Bacterial Genomes, 383–98. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-6369-3_31.

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Conference papers on the topic "Fragment restriction"

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Matthews, R. J., I. R. Peake, and A. L. Bloom. "POINT-MUTATION OF FACTOR VIII CODING SEQUENCES IN HAEMOPHILIA A." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644013.

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In order to study the molecular basis of haemophilia A, DNA from 26 haemophilia A patients (8 severe with inhibitors, 13 severe noninhibitors and 5 mild/moderate) was screened by the Southern blotting method with FVIII cDNA probe A (a i.7kb Kpnl cDNA fragment that spans exons 1 to 12) probe B (a 4.7kb EcoRI cDNA fragment that contains exons 14 to 25 and part of exon 26) probe C (a 1.8kb EcoRI cDNA fragment that contains the remainder of exon 26 and probe D (an Apal/EcoRI 783bp cDNA fragment that includes all of exons 22 to 25 and parts of exons 21 and 26). All cDNA probes were kindly provided by Genetics Institutes Inc.No large structural alterations of the FVIII gene were detected in any of the patients. However altered TaqI restriction sites within the coding regions of 3 patients were observed. DNA from patient 1 with severe haemophilia (VIIIAg < 0.1 u/dl, inhibitor negative) when probed with probe C showed a substitution of the normal 2.6kb TaqI and 4.5kb EcoRI fragments with novel 12kb TaqI and 11.5kb EcoRI fragments respectively. In addition he showed the normal 4kb Bglll fragment with probe C. A point mutation or small deletion (50bp is suspected to be present within exon 26.Patient 2 had severe haemophilia and a FVIII inhibitor of 12 units (Bethesda). DNA from patient 2 when probed with probe B revealed a novel 5.0kb TaqI fragment instead of the normal 2.2kb and 2.8kb fragments.The location of the altered Taq I restriction site within the coding region of exon 18 was confirmed with intragenomic probe pi 14.12 that includes exons 17and 18 (kindly provided by Genentech Inc.) A family study with this mutation specific fragment showed the patients sister and mother to be carriers.DNA from Patient 3 (severe haemophilia, factor VIII inhibitor 33 units) when probed independently with probes B and D revealed the absence of the normal 2.4kb and 1.4kb TaqI fragments and the generation of a novel 3.8kb TaqI fragment suggesting an alteration of the TaqI site within the coding region of exon 23.The detection of altered TaqI restriction sites in 3of our patients is further evidence that 'CG' dinucleotide sequences might be relative hot-spots for mutation when occurring within coding sequences of genes.
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Creignou, Nadia, Adrian Haret, Odile Papini, and Stefan Woltran. "Belief Update in the Horn Fragment." In Twenty-Seventh International Joint Conference on Artificial Intelligence {IJCAI-18}. California: International Joint Conferences on Artificial Intelligence Organization, 2018. http://dx.doi.org/10.24963/ijcai.2018/246.

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In line with recent work on belief change in fragments of propositional logic, we study belief update in the Horn fragment. We start from the standard KM postulates used to axiomatize belief update operators; these postulates lend themselves to semantic characterizations in terms of partial (resp. total) preorders on possible worlds. Since the Horn fragment is not closed under disjunction, the standard postulates have to be adapted for the Horn fragment. Moreover, a restriction on the preorders (i.e., Horn compliance) and additional postulates are needed to obtain sensible characterizations for the Horn fragment, and this leads to our main contribution: a representation result which shows that the class of update operators captured by Horn compliant partial (resp. total) preorders over possible worlds is precisely that given by the adapted and augmented Horn update postulates. With these results at hand, we provide concrete Horn update operators and are able to shed light on Horn revision operators based on partial preorders.
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Santana, M., and J. González. "Desulfovibrio vulgaris Hildenborough transcriptomic analysis by Restriction fragment functional display (RFFD)." In Proceedings of the II International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2007). WORLD SCIENTIFIC, 2009. http://dx.doi.org/10.1142/9789812837554_0119.

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Drury, Heather A., David G. Politte, John M. Ollinger, Philip Green, and Lewis J. Thomas, Jr. "Maximum-likelihood estimation of restriction-fragment mobilities from 1-D electrophoretic agarose gels." In SPIE/IS&T 1992 Symposium on Electronic Imaging: Science and Technology, edited by Raj S. Acharya, Carol J. Cogswell, and Dmitry B. Goldgof. SPIE, 1992. http://dx.doi.org/10.1117/12.59585.

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Zakharkina, TI, C. Herr, OA Yildirim, M. Friedrich, and R. Bals. "Terminal Restriction Fragment Length Polymorphism Is a Representative Tool To Study Pulmonary Microbial Communities." In American Thoracic Society 2009 International Conference, May 15-20, 2009 • San Diego, California. American Thoracic Society, 2009. http://dx.doi.org/10.1164/ajrccm-conference.2009.179.1_meetingabstracts.a3249.

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Standen, G., P. Moodie, H. Pannekoek, C. L. Verweij, and I. R. Peake. "ANALYSIS OF THE VON WILLEBRAND FACTOR (vWF) GENE IN 6 PATIENTS WITH SEVERE TYPE III VON WILLEBRANDS DISEASE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644641.

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DNA from 6 unrelated patients with severe type III von Willebrands disease (vWF antigen < 0.01u/dl) was studied with a cDNA probe for the 3' end of the vWF gene. DNA was extracted from peripheral blood leucocytes using standard techniques and was digested with a range of restriction enzymes. DNA fragments were separated by electrophoresis in 0.7% agarose and were southern blotted onto hybond-N (Amersham). The probe used was pvWF1100, a 1.1kb PstI fragment derived from the 2.28kb vWFcDNA insert of pvWF2280 isolated from a human endothelial cell cDNA expression library (Verweij et al, Nucleic Acids Res 13 (1985) 4699-4717). The probe corresponds to nucleotides 7083 to 8191 of the vWF cDNA (first nucleotide of initiator methionine as 1).When digested with Bglll and probed with pvWF11000, normal DNA showed two invariant bands (13 and 4.9kb) and polymorphic bands of 9 and/or 7.4kb. This pattern was also seen in 5 of the 6 severe vWD patients DNA suggesting that in this 3' area of the gene they had no major deletions or rearrangements. In the 6th case however the band of 4.9kb was not seen and did not appear to be replaced by any novel fragments, suggesting a partial deletion including some of the 3' end of the gene. This patient had the clinically severest form of the condition in that the patient had developed, some 10 years ago, an antibody (inhibitor) to vWF as detected by the ability of the patients plasma to inhibit restocetin cofactor activity in normal plasma. His parents were related (his mother was his father's second cousin) and had levels of vWFAg, considerably lower than those of factor VIII activity. This situation has been previously reported in carriers of recessive severe vWD. vWD was also present in a second family member, but in a less severe form (vWFAg 3u/dl). This patient and all other members of the family have, to date, given normal restriction fragment patterns with the vWF probe and several enzymes, including BgIII.
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Liddell, M. B., D. S. Anson, D. P. Lillicrap, and I. R. Peake. "SEARCH FOR AND USE OF RESTRICTION FRAGMENT LENGTH POLYMORPHISMS (RFLPs) IN AND AROUND THE HUMAN FACTOR IX GENE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644078.

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5 previously described RFLPs within the factor IX gene have been used for family studies (carrier detection) in 10 haemophilia B kindred. In all DNA from 91 individuals, including 25 obligate or possible carriers, was analysed by digestion with TaqI and XmnI and probing with the intragenomic probe VIII (all probes were provided by Professor G. G. Brownlee, Oxford). When noninformative, additional RFLPs (DdeI;probe XIII and MspI;probe II) were used. Of 12 possible carriers, 11 were diagnosed (6 as carriers, 5 normal). Of the confirmed carriers (6 diagnosed, 13 obligate) 15 were informative (heterozygous and phase known), and the overall incidence of heterozygosity was 72%. The recently reported BamHI RFLP was not found to be useful ( <1.0% frequency).Further RFLPs in and flanking the factor IX gene were sought by two procedures. Firstly cosmid pCHIXα, containing a 40kb insert including the 3' end of the factor IX gene and stretching some 35kb 3' to the gene was used as a large probe, with repetitive sequences being blocked by preannealing the probe with an excess of sonicated, denatured human DNA (Litt and White, PNAS 82, 6206). Results with 25 restriction enzymes (covering an estimated 1038 nucleotides) and DNA from 7 unrelated females were obtained, but only one low frequency PvuII RFLP (frequency about 1%) was identified. Similar experiments with further cosmid probes 3' to the gene are underway. The second technique was developed to analyse small DNA fragments (<1.0kb) generated by frequently cutting restriction enzymes. These fragments were separated on 3.5% polyacrylamide/0.5% agarose composite gels and then electroblotted onto hybond-N. Fragments of 150bp were readily visualised by this procedure. 3 frequently cutting enzymes have been used (Hinfl, Rsal and Mbol), and the blots probed with a factor IX c-DNA probe, or a unique sequence subclone of cosmid pCHIXα. To date no RFLPs have been identified. This search for further useful RFLP has illustrated the paucity of detectable sequence variation within this region of the X-chromosome.
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Ploos van Amstel, J. K., A. L. van der Zanden, P. H. Reitsma, and R. M. Bertina. "RESTRICTION ANALYSIS AND SOUTHERN BLOTTING OF TOTAL HUMAN DNA REVEALS THE EXISTENCE OF MORE THAN ONE GENE HOMOLOGOUS WITH PROTEIN S cDNA." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644639.

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A deficiency in protein S, the cofactor of activated protein C, is associated with an increased risk for the development of venous thrombosis. It is inherited as an autosomal dominant disorder. To improve the detection of heterozygotes in affected families, we have started to search for restriction fragment length polymorphism (RFLP) in the protein S gene. This study revealed the existence of two genes containing sequences homologous to protein S cDNA.Three non-overlapping fragments of clone pSUL5, which codes for the carboxy-terminal part of protein S and contains the complete 3' untranslated region, were isolated and used as probes in search for RFLP of the protein S gene.Surprisingly the non-overlapping probes shared more than one hybridizing band. The hybridization took place under stringent assay conditions.This observation is contradictory to the intron-exon organization of a gene and suggests the existence of two genes, containing sequences homologous with pSUL5. Both genes could be assigned to chromosome 3 by mapping through somatic cell hybrids. Whether two functional protein S genes are present in the human genome remains to be established.
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Chelucci, C., H. J. Hassan, R. Guerriero, A. Leonardi, G. Mattia, and C. Peschle. "POLYMORPHIC SITES IN FACTOR X GENE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643836.

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The structure of factor X gene has been analyzed by Southern blot in 5 subjects with factor X deficiency.Genomic DNA was digested with 8 different endonucleases and hybridized with a cDNA probe. The congenital deficiency observed in these patients is not apparently due to a major deletion or rearrangement. Since the gene locus is grossly intact, the disease presumably results from point mutation(s) not identified by the utilized endonucleases.Our study was also focused on the presence of polymorphic site(s) in the factor X gene locus. Analysis of 50 normal subjects allowed to identify several polymorphic restriction sites after digestion with EcoRI, Hind III and Pvu II.The restriction pattern obtained after Hind III digestion showed two bands of 7.3 and 6.0 Kb, while in two families an additional 7.6 Kb band was observed. Genomic DNA digested with EcoRI showed a 7.1 and 5.1 Kb fragments, and also a 6.6 Kb band with a 10% f requency.After DNA digestion with Pvu II 5.6, 2.7 and ∼1.0 Kb bands were observed. In three unrelated subjects we observed an additional 3.0 Kb fragment, in two other subjects a 3.5 kb band. Interestingly hybridization with a 178 bp cDNA subclone allowed to map the polymorphic sites in a the 3’ region of the gene locus.
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Haiying, Cheng, and Wang Weidong. "16S rRNA: Genes Comparative Analysis of Microbial Communities in Oil Reservoir With Nutrients Injection by Terminal Restriction Fragment Length Polymorphism Method." In International Symposium on Oilfield Chemistry. Society of Petroleum Engineers, 2007. http://dx.doi.org/10.2118/106398-ms.

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Reports on the topic "Fragment restriction"

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Stevens, Tracy. Analysis of mitochondrial DNA restriction fragment patterns in killer whales, Orcinus orca. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.5812.

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Braun, B., W. Blanch, and J. M. Prausnitz. Capillary electrophoretic separation of DNA restriction fragments using dilute polymer solutions. Office of Scientific and Technical Information (OSTI), February 1997. http://dx.doi.org/10.2172/453768.

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Hamann, Franz, Cesar Anzola, Oscar Avila-Montealegre, Juan Carlos Castro-Fernandez, Anderson Grajales-Olarte, Alexander Guarín, Juan C. Mendez-Vizcaino, Juan J. Ospina-Tejeiro, and Mario A. Ramos-Veloza. Monetary Policy Response to a Migration Shock: An Analysis for a Small Open Economy. Banco de la República de Colombia, January 2021. http://dx.doi.org/10.32468/be.1153.

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We develop a small open economy model with nominal rigidities and fragmented labor markets to study the response of the monetary policy to a migration shock. Migrants are characterized by their productivity levels, their restrictions to accumulate capital, as well as by the flexibility of their labor income. Our results show that the monetary policy response depends on the characteristics of migrants and the local labor market. An inflow of low(high)-productivity workers reduces(increases) marginal costs, lowers(raises) inflation expectations and pushes the Central Bank to reduce(increase) the interest rate. The model is calibrated to the Colombian economy and used to analyze a migratory inflow of financially constraint workers from Venezuela into a sector with flexible and low wages.
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