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Journal articles on the topic 'Fragments of standards'

Author: Grafiati

Published: 28 May 2022

Last updated: 31 July 2025

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1

Zhao, Minrui, Hongni Gao, Qi Han, Jiaang Ge, Wei Wang, and Jue Qu. "Development of a Driving Cycle for Fuzhou Using K-Means and AMPSO." Journal of Advanced Transportation 2021 (February 24, 2021): 1–15. http://dx.doi.org/10.1155/2021/5430137.

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The driving cycle is a speed-to-time curve, a fundamental technique in the automotive industry, and also a basis to set standards for fuel consumption and emissions of vehicles. A driving cycle is developed based on firsthand driving data collected from fieldwork. First, bad data in the original dataset are preprocessed, the time-series standard smoothing algorithm is used to smoothen the data, and Lagrange’s interpolation is used to realize data interpolation. Next, the rules for kinematic fragment extraction are set to divide the data into kinematic fragments. Last, an evaluation system of k

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Nardulli, A. M., and D. J. Shapiro. "Binding of the estrogen receptor DNA-binding domain to the estrogen response element induces DNA bending." Molecular and Cellular Biology 12, no. 5 (1992): 2037–42. http://dx.doi.org/10.1128/mcb.12.5.2037-2042.1992.

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We have used circular permutation analysis to determine whether binding of purified Xenopus laevis estrogen receptor DNA-binding domain (DBD) to a DNA fragment containing an estrogen response element (ERE) causes the DNA to bend. Gel mobility shift assays showed that DBD-DNA complexes formed with fragments containing more centrally located EREs migrated more slowly than complexes formed with fragments containing EREs near the ends of the DNA. DNA bending standards were used to determine that the degree of bending induced by binding of the DBD to an ERE was approximately 34 degrees. A 1.55-fold

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Nardulli, A. M., and D. J. Shapiro. "Binding of the estrogen receptor DNA-binding domain to the estrogen response element induces DNA bending." Molecular and Cellular Biology 12, no. 5 (1992): 2037–42. http://dx.doi.org/10.1128/mcb.12.5.2037.

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We have used circular permutation analysis to determine whether binding of purified Xenopus laevis estrogen receptor DNA-binding domain (DBD) to a DNA fragment containing an estrogen response element (ERE) causes the DNA to bend. Gel mobility shift assays showed that DBD-DNA complexes formed with fragments containing more centrally located EREs migrated more slowly than complexes formed with fragments containing EREs near the ends of the DNA. DNA bending standards were used to determine that the degree of bending induced by binding of the DBD to an ERE was approximately 34 degrees. A 1.55-fold

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Shoukry, Mira, Andrea Lopez-Ruiz, Dharmendra Patel, and Alexander Shusko. "Retained lens fragment causing anterior uveitis four years after successful phacoemulsification." Journal of Cataract & Refractive Surgery Online Case Reports 13 (January 2025): e00146. https://doi.org/10.1097/j.jcro.0000000000000146.

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Introduction: Existing literature has detailed occurrences of retained cataract lens fragments identified days to weeks after cataract surgery. However, lens fragments in the anterior chamber remaining asymptomatic for several years are extremely rare, thus remaining a diagnostic challenge. A complex case of a retained lens fragment causing anterior uveitis 4 years after phacoemulsification is presented. Patient and Clinical Findings: A 76-year-old woman presented with a chief concern of right eye pain, photosensitivity, and blurred vision after head trauma. Diagnosis, Intervention, and Outcom

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Andryukhin, Michail I., Sergey A. Golovanov, Anastasia M. Polikarpova, et al. "Staghorn stones’ composition analysis features." Pediatrician (St. Petersburg) 8, no. 5 (2017): 61–66. http://dx.doi.org/10.17816/ped8561-66.

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The lack of standards in the analysis of the chemical composition of the staghorn stones leads to a decrease in the effectiveness of metaphylaxis, and especially in those cases where the volume of the stone is much larger than the volume of the stone fragment being studied. The aim of this study was to develop standards in order to determine the composition of the staghorn calculi. In the Institute of urology from 2015 to 2016, we identified patients with urolithiasis, staghorn-stone nephrolithiasis who were eventually hospitalized. All patients underwent percutaneous nephrolithotripsy, and fr

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Miller, Spencer, Carlo Caruso, and Crawford Drury. "Validating the Precision and Accuracy of Coral Fragment Photogrammetry." Remote Sensing 16, no. 22 (2024): 4274. http://dx.doi.org/10.3390/rs16224274.

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Photogrammetry is a common tool for evaluating ecosystem-scale questions on coral reefs due to the ability to measure complex structures in situ. This technique is also increasingly being used at smaller scales to collect growth and morphometric data about individual coral fragments in manipulative experiments. However, there are substantial uncertainties in data quality and interpretation and limited reporting of the parameters useful for standardization across studies. There is a need to characterize the capabilities of photogrammetry as applied to coral fragments, to establish validation me

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Bhure, Ujwal, Christoph Agten, Dirk Lehnick, et al. "Value of SPECT/CT in the assessment of necrotic bone fragments in patients with delayed bone healing or non-union after traumatic fractures." British Journal of Radiology 93, no. 1114 (2020): 20200300. http://dx.doi.org/10.1259/bjr.20200300.

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Objective: To evaluate the value of single-photon emission computed tomography / computed tomography (SPECT/CT) compared with planar bone scintigraphy (BS) in the assessment of necrotic bone fragments in patients with delayed bone healing or non-union after traumatic fractures. Methods: Retrospective evaluation of patients with traumatic fractures and suspected delayed healing or non-union and/or persistent pain or suspected infection who had undergone planar late phase BS and SPECT/CT between 2011 and 2018. On the BS and SPECT/CT images, a necrotic fragment was considered if there was an area

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Bhure, Ujwal, Christoph Agten, Dirk Lehnick, et al. "Value of SPECT/CT in the assessment of necrotic bone fragments in patients with delayed bone healing or non-union after traumatic fractures." British journal of radiology 93, no. 1114 (2020): 1, 7. https://doi.org/10.5281/zenodo.4626969.

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OBJECTIVE To evaluate the value of single-photon emission computed tomography / computed tomography (SPECT/CT) compared with planar bone scintigraphy (BS) in the assessment of necrotic bone fragments in patients with delayed bone healing or non-union after traumatic fractures. METHODS Retrospective evaluation of patients with traumatic fractures and suspected delayed healing or non-union and/or persistent pain or suspected infection who had undergone planar late phase BS and SPECT/CT between 2011 and 2018. On the BS and SPECT/CT images, a necrotic fragment was considered if there was an area o

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Garcés-Garcés, José, Marta Redrado, Ángela Sastre-Santos, María Concepción Gimeno, and Fernando Fernández-Lázaro. "Synthesis of Dipyridylaminoperylenediimide–Metal Complexes and Their Cytotoxicity Studies." Pharmaceutics 14, no. 12 (2022): 2616. http://dx.doi.org/10.3390/pharmaceutics14122616.

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A new family of perylenediimide (PDI) silver and copper complexes has been successfully synthesized by reacting ortho- and bay-substituted (dipyrid-2′,2″-ylamino)perylenediimide ligands with metal phosphine fragments. The coordination of the metal center did not reveal a significant effect on the photophysical properties, which are mainly due to the PDI ligands, and in some cases quenching of the luminescence was observed. The antiproliferative effect of the free perylenediimide ligands and the metalloPDI complexes against the cervix cancer cell line HeLa was determined by MTT assay. The free

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Gerner-Smidt, P., L. M. Graves, Susan Hunter, and B. Swaminathan. "Computerized Analysis of Restriction Fragment Length Polymorphism Patterns: Comparative Evaluation of Two Commercial Software Packages." Journal of Clinical Microbiology 36, no. 5 (1998): 1318–23. http://dx.doi.org/10.1128/jcm.36.5.1318-1323.1998.

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Two computerized restriction fragment length polymorphism pattern analysis systems, the BioImage system and the GelCompar system (Molecular Analyst Fingerprinting Plus in the United States), were compared. The two systems use different approaches to compare patterns from different gels. In GelCompar, a standard reference pattern in one gel is used to normalize subsequent gels containing lanes with the same reference pattern. In BioImage, the molecular sizes of the fragments are calculated from size standards present in each gel. The molecular size estimates obtained with the two systems for 12

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Maksimyuk, Elena, and Elena Tsoupikova. "Male Behaviour Standards in Female Perception." SHS Web of Conferences 50 (2018): 01101. http://dx.doi.org/10.1051/shsconf/20185001101.

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The paper is devoted to the analysis of male behaviour standards in female perception. Speech fragments taken from female dialogs explicate the presence of male behaviour standards in female consciousness. Cognitive and semantic analysis of female speech behavior peculiarities allowed us to reconstruct and describe female perception determined by social and cultural reality of linguistic and cultural society they live in. It was discovered that female perception was determined by social and cultural stereotypes and roles. This determination is realized in a strong need to correspond to these s

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Naumann, A., A. K. Scherger, J. Neuwirth, et al. "Selection and characterisation of FVIII-specific single chain variable fragments." Hämostaseologie 33, S 01 (2013): S39—S45. http://dx.doi.org/10.1055/s-0037-1619801.

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SummaryThe development of inhibitory anti-FVIII antibodies is currently the most severe complication in the treatment of haemophilia A patients. Inhibitor eradication can be achieved by immune tolerance induction (ITI). Recent findings suggest a correlation between the FVIII-specific IgG subclass distribution and the duration or outcome of ITI. To quantify FVIII-specific IgG subclasses in patients’ plasma FVIII-specific IgG standards are required. Here, the isolation of FVIII-specific single chain variable fragments (scFvs) from synthetic phage display libraries and the characterisation of the

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Yang, Kun, Giovanni Stracquadanio, Jingchuan Luo, Jef D. Boeke, and Joel S. Bader. "BioPartsBuilder: a synthetic biology tool for combinatorial assembly of biological parts." Bioinformatics 32, no. 6 (2015): 937–39. http://dx.doi.org/10.1093/bioinformatics/btv664.

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Abstract Summary: Combinatorial assembly of DNA elements is an efficient method for building large-scale synthetic pathways from standardized, reusable components. These methods are particularly useful because they enable assembly of multiple DNA fragments in one reaction, at the cost of requiring that each fragment satisfies design constraints. We developed BioPartsBuilder as a biologist-friendly web tool to design biological parts that are compatible with DNA combinatorial assembly methods, such as Golden Gate and related methods. It retrieves biological sequences, enforces compliance with a

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Gong, Kenan, Keqin Gan, and Aixian Li. "Method and practice of comparing indicators from standards." MATEC Web of Conferences 232 (2018): 03033. http://dx.doi.org/10.1051/matecconf/201823203033.

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Chinese standards and foreign standards differ in technical indicators and requirements, which result in the poor recognition of products that comply with Chinese standards. It is crucial to set up a universal method to compare standard indicators, so that the inconsistency between standards could be eliminated, and ultimately improve Chinese products competitiveness. This paper proposed a method for indicator comparison from standards. Based on Semantic Web technology, the method introduced fragmentation of standard documents in a manner of ‘product stylistic rule indicator’ triplet. The proc

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Stafford, Prachi, Cedric Ghevaert, Kate Campbell та ін. "Recombinant Mini-β3 Integrin Fragments for the Detection of HPA-1 Antibodies." Blood 106, № 11 (2005): 218. http://dx.doi.org/10.1182/blood.v106.11.218.218.

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Abstract The αIIbβ3 integrin (GPIIbIIIa, CD61) is the platelet receptor for fibrinogen, fibronectin and vitronectin. A single nucleotide polymorphism (dbSNP Id= rs5918) in the ITGB3 gene defines the Human Platelet Antigen (HPA)-1 system encoding either a Leucine (1a) or Proline (1b) at position 33. HPA-1a immunisation is of clinical relevance in neonatal alloimmune thrombocytopenia (NAIT), post transfusion purpura (PTP) and platelet refractoriness. Currently, the detection of HPA-1 antibodies is based on a cumbersome sandwich ELISA requiring HPA genotyped platelets as source of antigen. We set

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Khmeleva, S. A., L. K. Kurbatov, K. G. Ptitsyn, et al. "Identification of Ribosomal Footprints on the Electrophoretic Gel in Translatome Profiling: on the Use of DNA Size Standards." Biomedical Chemistry: Research and Methods 6, no. 3 (2023): e00195. http://dx.doi.org/10.18097/bmcrm00195.

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The commercial DNA ladder was tested as a substitute for RNA size standards to identify ribosomal footprints (RNA fragments of about 30 nucleotides long) on an electrophoretic polyacrylamide gel for the purposes of translatome profiling. It has been found that 25 and 35 nucleotides long synthetic RNA oligonucleotides do migrate slower than the synthetic DNA oligonucleotides of the matching length and sequences and their positions on the gel coincide with those of 30 and 40 nucleotides long DNA oligonucleotides, correspondingly, of the commercial IDT 20/100 DNA oligo length standards. By using

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KHALAF, RANIA, OLIVER KOPP, and FRANK LEYMANN. "MAINTAINING DATA DEPENDENCIES ACROSS BPEL PROCESS FRAGMENTS." International Journal of Cooperative Information Systems 17, no. 03 (2008): 259–82. http://dx.doi.org/10.1142/s0218843008001828.

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Continuous process improvement (CPI) may require a BPEL process to be split amongst different participants. In this paper, we enable splitting standard BPEL — without requiring any new middleware for the case of flat flows. The solution also supports splitting loops and scopes that have compensation and/or fault handlers. When splitting loops and scopes, we extend existing Web services standards and frameworks in a standard compliant manner in order to support the resulting split control (not data) between the fragments. Data dependencies, however, are handled directly using BPEL constructs pl

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Gillespie, John, and Ha H Do. "THEORISING THE LOCAL ADAPTATION OF TRANSNATIONAL CERTIFICATION STANDARDS." International and Comparative Law Quarterly 71, no. 1 (2021): 37–71. http://dx.doi.org/10.1017/s0020589321000439.

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AbstractOver the last three decades, transnational certification standards have proliferated to fill perceived ‘governance gaps’ in developing countries. Transnational non-governmental organisations and private standards-setting agencies have developed standards that cover a vast range of areas such as labour rights, social justice and environmental protection. As a form of private transnational regulation, certification standards travel through transnational production networks that link lead firms in developed countries with supplier firms in developing countries. This article draws on a cas

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Mayrand, P. E., L. B. Hoff, L. J. McBride, et al. "Automation of specific human gene detection." Clinical Chemistry 36, no. 12 (1990): 2063–71. http://dx.doi.org/10.1093/clinchem/36.12.2063.

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Abstract An instrument/chemistry system is described that automates a new chemical procedure functionally equivalent to Southern blotting. A fluorescence gel scanner that detects migrating DNA fragments in real-time analyzes the samples produced by a prototype liquid-handling instrument that automates a solution-phase hybridization/solid-phase capture chemistry for DNA analysis. The combination of this chemistry, the gel scanner, and robotic automation eliminates the tedium encountered in traditional manual methods for specific gene detection and reduces analysis time from days to hours. Restr

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Wehmann, R. E., D. L. Blithe, A. H. Akar та B. C. Nisula. "β-Core fragments are contaminants of the World Health Organization Reference Preparations of human choriogonadotrophin and its α-subunit". Journal of Endocrinology 117, № 1 (1988): 147–52. http://dx.doi.org/10.1677/joe.0.1170147.

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ABSTRACT Highly purified preparations of human choriogonadotrophin (hCG), hCGα and hCGβ, including those preparations which are being distributed by the World Health Organization as International Standards, cross-reacted in a new radioimmunoassay with increased relative specificity for the β-core fragment of hCG. A major portion of the β-core immunoreactivity in the hCG and hCGα preparations eluted from Sephadex G-100 in a position (approximate apparent molecular size 15 000–18 000) corresponding to that of purified β-core fragment prepared from pregnancy urine. However, in the case of hCGβ-su

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Alkaff, Ahmad Husein, Mutiara Saragih, Shabrina Noor Imana, Mochammad Arfin Fardiansyah Nasution, and Usman Sumo Friend Tambunan. "Identification of DNA Methyltransferase-1 Inhibitor for Breast Cancer Therapy through Computational Fragment-Based Drug Design." Molecules 26, no. 2 (2021): 375. http://dx.doi.org/10.3390/molecules26020375.

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Epimutation by DNA Methyltransferase 1 (DNMT1), an epigenetic regulator enzyme, may lead to the proliferation of breast cancer. In this report, 168,686 natural products from the PubChem database were screened and modified by in silico method to acquire the potential inhibitor of DNMT1. The initial screening of PubChem natural products using Lipinski’s and Veber’s rules of three and toxic properties have resulted in 2601 fragment candidates. Four fragments from pharmacophore-based molecular docking simulation were modified by utilizing FragFP and the Lipinski’s and Veber’s rules of five, and re

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Ueno, Saneyoshi, Yoshihiko Tsumura, and Izumi Washitani. "Cost-Effective Method to Synthesize Fluorescently Labeled DNA Size Standards Using Cloned AFLP Fragments." BioTechniques 34, no. 6 (2003): 1146–48. http://dx.doi.org/10.2144/03346bm05.

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Jin, C. F., M. Mata, and D. J. Fink. "Rapid construction of deleted DNA fragments for use as internal standards in competitive PCR." Genome Research 3, no. 4 (1994): 252–55. http://dx.doi.org/10.1101/gr.3.4.252.

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Peri, Saketh R., Forhad Akhter, Robert A. De Lorenzo, and R. Lyle Hood. "Portable Medical Suction and Aspirator Devices: Are the Design and Performance Standards Relevant?" Sensors 22, no. 7 (2022): 2515. http://dx.doi.org/10.3390/s22072515.

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Airway clearance refers to the clearing of any airway blockage caused due to foreign objects such as mud, gravel, and biomaterials such as blood, vomit, or teeth fragments using the technology of choice, portable suction devices. Currently available devices are either too heavy and bulky to be carried, or insufficiently powered to be useful despite being in accordance with the ISO 10079-1 standards. When applied to portable suction, the design and testing standards lack clinical relevancy, which is evidenced by how available portable suction devices are sparingly used in pre-hospital situation

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Valdes, Roland, Saeed A. Jortani, and Mihai Gheorghiade. "Standards of laboratory practice: cardiac drug monitoring." Clinical Chemistry 44, no. 5 (1998): 1096–109. http://dx.doi.org/10.1093/clinchem/44.5.1096.

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Abstract In this Standard of Laboratory Practice we recommend guidelines for therapeutic monitoring of cardiac drugs. Cardiac drugs are primarily used for treatment of angina, arrhythmias, and congestive heart failure. Digoxin, used in congestive heart failure, is widely prescribed and therapeutically monitored. Monitoring and use of antiarrhythmics such as disopyramide and lidocaine have been steadily declining. Immunoassay techniques are currently the most popular methods for measuring cardiac drugs. Several reasons make measurement of cardiac drugs in serum important: their narrow therapeut

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Settanni, Luca, Sara Valmorri, Douwe van Sinderen, Giovanna Suzzi, Antonello Paparella, and Aldo Corsetti. "Combination of Multiplex PCR and PCR-Denaturing Gradient Gel Electrophoresis for Monitoring Common Sourdough-Associated Lactobacillus Species." Applied and Environmental Microbiology 72, no. 5 (2006): 3793–96. http://dx.doi.org/10.1128/aem.72.5.3793-3796.2006.

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ABSTRACT A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtained from Lactobacillus type strains acted as standards to analyze lactobacilli from four regional Abruzzo (central Italy) sourdoughs.

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Dzhumaev, Sh Sh, Yu G. Borisova, G. Z. Raskil’dina, U. Sh Kuzmina, R. R. Daminev, and S. S. Zlotskii. "Synthesis of ethers containing 1,3-dioxolane and gem-dichlorocyclopropane fragments." Fine Chemical Technologies 16, no. 2 (2021): 156–66. http://dx.doi.org/10.32362/2410-6593-2021-16-2-156-166.

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Objectives. This study aimed to obtain ethers containing gem-dichlorocyclopropane and 1,3-dioxolane fragments and evaluate their cytotoxic properties against HEK293, SH-SY5Y, MCF-7, and A549 cell lines.Methods. The qualitative and quantitative compositions of the reaction masses were determined using mass spectrometry (using a Chromatek-Kristall 5000M device with the 2012 National Institute of Standards and Technology, USA database) and nuclear magnetic resonance spectroscopy (using a Bruker AM-500 device with operating frequencies of 500 and 125 MHz).Results. Ethers containing gem-dichlorocyc

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Sesay, Manso, Jamal Rostami, Syd Slouka, Hugh Miller, Rennie Kaunda, and Anshuman Mohanty. "The Influence of Conical Pick Cutter Wear Conditions on Physical Characteristics and Particle Size Distribution of Coal: Health and Safety Considerations with a Focus on Silica." Minerals 15, no. 2 (2025): 182. https://doi.org/10.3390/min15020182.

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This study investigates the correlations between the wear conditions of conical pick cutters and key variables such as the physical properties (shape, aspect ratio, roughness), explosive potential, health and safety implications, and particle size distribution of coal dust and larger fragments using the linear cutting machine (LCM). This research was conducted within the framework of recent regulatory developments, notably implementing the new silica rule in the mining and construction sectors and climate change consideration. This study reveals critical insights into optimizing operational pr

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Novokhatko, Anna. "Epicharmus’ Comedy and Early Sicilian Scholarship." Scripta Classica Israelica 34 (February 15, 2020): 69–84. https://doi.org/10.71043/sci.v34i.2343.

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Some fragments of Epicharmus are examined in the context of contemporary scholarly discourse, especially with regard to textual and literary criticism, grammar and stylistics as they developed in the Sicily of his time. The interaction of the comedy of Epicharmus with scholarship is complex and involves a reflection and revision of contemporary ideas, but also a process of literary differentiation. This new genre was created through a critical interaction with other genres as part of a process of reinterpretation and literary exegesis. Furthermore, the fragments discussed offer the possibility

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Pratama, Akbar Adjie, Benjamin Bolduc, Ahmed A. Zayed, et al. "Expanding standards in viromics: in silico evaluation of dsDNA viral genome identification, classification, and auxiliary metabolic gene curation." PeerJ 9 (June 14, 2021): e11447. http://dx.doi.org/10.7717/peerj.11447.

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Background Viruses influence global patterns of microbial diversity and nutrient cycles. Though viral metagenomics (viromics), specifically targeting dsDNA viruses, has been critical for revealing viral roles across diverse ecosystems, its analyses differ in many ways from those used for microbes. To date, viromics benchmarking has covered read pre-processing, assembly, relative abundance, read mapping thresholds and diversity estimation, but other steps would benefit from benchmarking and standardization. Here we use in silico-generated datasets and an extensive literature survey to evaluate

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Kaur, Manpreet, and Hayat M. Mukhtar. "PHARMACOGNOSTICAL STANDARDIZATION OF RHIZOMES OF NUPHAR LUTEUM." Asian Journal of Pharmaceutical and Clinical Research 10, no. 8 (2017): 267. http://dx.doi.org/10.22159/ajpcr.2017.v10i8.19030.

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Objective: This study provides the detailed summary of pharmacognostical characters of rhizomes to give clear standards for the identification ofdrug.Methods: Important details such as macroscopy, microscopy (transverse section [TS] and powder microscopy) along with physico-chemicalparameters (such as loss on drying, ash values, and extractive values), and quantitative studies (i.e foreign matter, length and width of xylem vesselsand calcium oxalate crystals, and diameter of starch grains) were performed by standard procedure.Results: The findings of macroscopic studies revealed that the rhizo

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McCoy, Matthew S., Karin Rolanda Jongsma, Phoebe Friesen, et al. "National Standards for Public Involvement in Research: missing the forest for the trees." Journal of Medical Ethics 44, no. 12 (2018): 801–4. http://dx.doi.org/10.1136/medethics-2018-105088.

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Biomedical research funding bodies across Europe and North America increasingly encourage—and, in some cases, require—investigators to involve members of the public in funded research. Yet there remains a striking lack of clarity about what ‘good’ or ‘successful’ public involvement looks like. In an effort to provide guidance to investigators and research organisations, representatives of several key research funding bodies in the UK recently came together to develop the National Standards for Public Involvement in Research. The Standards have critical implications for the future of biomedical

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Fey, Axel, Stefan Eichler, SÃ¯Â¿Â½bastien Flavier, Richard Christen, Manfred G. HÃ¯Â¿Â½fle, and Carlos A. GuzmÃ¯Â¿Â½n. "Establishment of a Real-Time PCR-Based Approach for Accurate Quantification of Bacterial RNA Targets in Water, Using Salmonella as a Model Organism." Applied and Environmental Microbiology 70, no. 6 (2004): 3618–23. http://dx.doi.org/10.1128/aem.70.6.3618-3623.2004.

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ABSTRACT Quantitative PCR (Q-PCR) is a fast and efficient tool to quantify target genes. In eukaryotic cells, quantitative reverse transcription-PCR (Q-RT-PCR) is also used to quantify gene expression, with stably expressed housekeeping genes as standards. In bacteria, such stable expression of housekeeping genes does not occur, and the use of DNA standards leads to a broad underestimation. Therefore, an accurate quantification of RNA is feasible only by using appropriate RNA standards. We established and validated a Q-PCR method which enables the quantification of not only the number of copie

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Lim, Jongguk, Ahyeong Lee, Jungsook Kang, et al. "Non-Destructive Detection of Bone Fragments Embedded in Meat Using Hyperspectral Reflectance Imaging Technique." Sensors 20, no. 14 (2020): 4038. http://dx.doi.org/10.3390/s20144038.

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Meat consumption has shifted from a quantitative to a qualitative growth stage due to improved living standards and economic development. Recently, consumers have paid attention to quality and safety in their decision to purchase meat. However, foreign substances which are not normal food ingredients are unintentionally incorporated into meat. These should be eliminated as a hazard to quality or safety. It is important to find a fast, non-destructive, and accurate detection technique of foreign substance in the meat processing industry. Hyperspectral imaging technology has been regarded as a n

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Szleszyński, Bartłomiej. "Bolesław Prus i pozytywistyczne "porno z Murzynkami" [Bolesław Prus and a positivist "porn with Negro women"]." Napis XVIII (2012) (December 31, 2012): 155–73. https://doi.org/10.18318/napis.2012.1.10.

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The article begins with stating a fact that in Bolesław Prus’s Kroniki there are many fragments (especially those, in which black women occur), which sound so obscene that, according to contemporary standards, they could be considered pornographic. This lead to a conclusion that the conviction about an absolute prudery of the contemporary official circles and a current image of Bolesław Prus has to be modified. The next part of the article is devoted to an attempt to find the reasons why exactly this topic of African women provoked the author of Kroniki to come up with erotic fantasies (

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Li, Y., C. Y. S. Ku, J. Xu, D. Saxena, and P. W. Caufield. "Survey of Oral Microbial Diversity using PCR-based Denaturing Gradient Gel Electrophoresis." Journal of Dental Research 84, no. 6 (2005): 559–64. http://dx.doi.org/10.1177/154405910508400614.

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Polymicrobial biofilms in the human oral cavity exhibit marked diversity. PCR-based denaturing gradient gel electrophoresis (PCR-DGGE) surveys microbial diversity by displaying PCR-generated 16S rDNA fragments that migrate at different distances, reflecting the differences in the base-pair ( i.e., % G+C) composition of the fragment. This study examined DGGE-generated diversity profiles of cultivable bacteria from individuals with different caries status. Initially, we developed a set of PCR-DGGE running conditions appropriate to oral bacteria. Next, we assessed migration standards from known o

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Dellero, Younès, Olivier Filangi, and Alain Bouchereau. "Evaluation of GC/MS-Based 13C-Positional Approaches for TMS Derivatives of Organic and Amino Acids and Application to Plant 13C-Labeled Experiments." Metabolites 13, no. 4 (2023): 466. http://dx.doi.org/10.3390/metabo13040466.

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Analysis of plant metabolite 13C-enrichments with gas-chromatography mass spectrometry (GC/MS) has gained interest recently. By combining multiple fragments of a trimethylsilyl (TMS) derivative, 13C-positional enrichments can be calculated. However, this new approach may suffer from analytical biases depending on the fragments selected for calculation leading to significant errors in the final results. The goal of this study was to provide a framework for the validation of 13C-positional approaches and their application to plants based on some key metabolites (glycine, serine, glutamate, proli

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Sitarz, Mirosław. "Warsaw Mayor’s Position Regarding the Christian Cross." Teka Komisji Prawniczej PAN Oddział w Lublinie 17, no. 2 (2024): 409–14. https://doi.org/10.32084/tkp.9247.

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The article examines the position taken by the Mayor of Warsaw, Poland’s capital city, regarding the symbol of the Cross. First, the Ordinance of 8 May 2024 on the introduction of the Standards for Equal Treatment in the Warsaw City Hall is described. Second, the author cites relevant fragments of Sejm and Senate resolutions that informed the debate in defence of the Cross in the wake of the 2009 ruling of the European Court of Human Rights – resolutions that are still relevant given the current sociopolitical circumstances.

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Vereschaka, T. V., and A. A. Gorevalova. "Navigational nautical charts: a retrospective view at information potential." Geodesy and Cartography 971, no. 5 (2021): 39–50. http://dx.doi.org/10.22389/0016-7126-2021-971-5-39-50.

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The article explore more than a century of nautical charts’ content evolution based on the symbols, published in 1910, 1937, 1961, 1971, 1985 and 2016. This paper presents an analysis of the structure and the “general remarks“ to the publications and describes trends of the symbols correspondence to the standards of the International Hydrographic Organization. The ways of optimizing the structure and the content of charts based on the examples of the coastline and the bottom sediment are proposed . The information on electronic navigation charts, visualization features of mapping objects on th

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Štaffenová, Daniela, Pavol Durica, Peter Juras, and Ján Rybárik. "Evaluative Case Study in Lightweight Wooden Wall Research." Applied Mechanics and Materials 887 (January 2019): 56–63. http://dx.doi.org/10.4028/www.scientific.net/amm.887.56.

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This presented article deals with two newly designed experimental walls with different measuring sections (5 for each lightweight wooden wall). All fragments meet the standards required for zero and energy-plus buildings, which would be mandatory in Slovakia from the year 2020. The experimental walls are located in central Europe (town Zilina, Slovakia). Description of individual test sections, the measuring technology, data collection, interior technical equipment and outdoor boundary conditions monitoring are introduced. In the future, the experimental research should progressively combine t

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Xu, Like, Hannah Chen, Melisa Canales, and Lena Ciric. "Use of synthesized double-stranded gene fragments as qPCR standards for the quantification of antibiotic resistance genes." Journal of Microbiological Methods 164 (September 2019): 105670. http://dx.doi.org/10.1016/j.mimet.2019.105670.

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Patron, Nicola J. "Blueprints for green biotech: development and application of standards for plant synthetic biology." Biochemical Society Transactions 44, no. 3 (2016): 702–8. http://dx.doi.org/10.1042/bst20160044.

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Synthetic biology aims to apply engineering principles to the design and modification of biological systems and to the construction of biological parts and devices. The ability to programme cells by providing new instructions written in DNA is a foundational technology of the field. Large-scale de novo DNA synthesis has accelerated synthetic biology by offering custom-made molecules at ever decreasing costs. However, for large fragments and for experiments in which libraries of DNA sequences are assembled in different combinations, assembly in the laboratory is still desirable. Biological asse

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Li, Yin, and Guoqiang Deng. "Assessment of the lethal effect of a 500 kg deep-earth penetrating warhead striking underground confined space." Journal of Physics: Conference Series 2891, no. 6 (2024): 062006. https://doi.org/10.1088/1742-6596/2891/6/062006.

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Abstract As a priority military facility, underground constructions are high-value targets for earth-penetrating warheads. The lethal effect is different from that of open areas due to the confined internal space. In this study, numerical simulation and theoretical calculation are used to quantitatively and comparatively evaluate the performance of a 500-kg deep earth penetrating warhead charged with TNT, PBXN-109, and AFX-757 in a specific underground fortification. This evaluation considers the lethality of the shock wave, thermal effects, and case fragments, and the experimental data verify

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Ivanova, Yulia B., Olga A. Dmitrieva, Nugzar Zh Mamardashvili, Alexander S. Semeykin, Svetlana G. Pukhovskaya, and Mikolay M. Kruk. "FLUORESCENT PROPERTIES OF PHENYL - CONTAINING ISOMERS OF PALLADIUM COMPLEXES OF OCTAETHYLPORPHIN IN ACETONITRILE." IZVESTIYA VYSSHIKH UCHEBNYKH ZAVEDENII KHIMIYA KHIMICHESKAYA TEKHNOLOGIYA 63, no. 1 (2019): 71–77. http://dx.doi.org/10.6060/ivkkt.20206301.6125.

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The fluorimetric measurements of the acetonitrile solutions of palladium complexes of the octaethylporphyrin, 5,10- biphenyl octaethylporphyrin and 5,15-biphenyl octaethylporphyrin at 293 K were performed. Tetraphenylporfin was used in toluene as standards. It was determined that the studied compounds differ in the architecture of peripheral substitution, and as a result in molecular conformation and symmetry, which determine the rates of intramolecular processes of deactivation of electronic excitation energy. It was established that the introduction of two phenyl fragments into the meso-posi

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Huo, Yanghui, Kailin Li, Suyu Yang, et al. "A Systematic Methodology for the Identification of the Chemical Composition of the Mongolian Drug Erdun-Uril Compound Utilizing UHPLC-Q-Exactive Orbitrap Mass Spectrometry." Molecules 29, no. 18 (2024): 4349. http://dx.doi.org/10.3390/molecules29184349.

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The traditional Mongolian medicine Erdun-Uril is a conventional combination of 29 herbs commonly used for the treatment of cerebrovascular ailments. It has the effects of reducing inflammation, counteracting oxidative stress, and averting strokes caused by persistent cerebral hypoperfusion. Prior research on Erdun-Uril has predominantly concentrated on its pharmacodynamics and mechanism of action; however, there has been a lack of systematic and comprehensive investigation into its chemical constituents. Therefore, it is crucial to establish an efficient and rapid method for evaluating the che

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Lamboy, Warren F., Christopher A. Alpha, and David V. Peterson. "Unknown Cultivars of Cold-hardy Grape Can Be Successfully Identified by Their Simples Sequence Repeat (SSR) Fingerprints." HortScience 33, no. 3 (1998): 516d—517. http://dx.doi.org/10.21273/hortsci.33.3.516d.

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Simple sequence repeat DNA fragments (SSRs) have been suggested as the method of choice for DNA fingerprinting of grape cultivars. Nevertheless, the use of SSRs as a practical fingerprinting method is not without its pitfalls. For example, when the polymerase chain reaction is used to amplify SSR sequences, potentially confusing “stutter” bands may occur, or there may be non-template directed addition of an “A” to the end of synthesized fragments, or other artifactual amplification products may be produced. Since we would like to fingerprint our entire cold-hardy grape collection of ≈1300 cult

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Dinh, Hang Thi Thu, Mai Ngoc Nguyen, and Su Xuan Hoang. "In-vitro synthesis of RNA fragments specifying envelope protein gene and RdRp gene of SARS-CoV-2 as positive standards for molecular diagnosis tests." Ministry of Science and Technology, Vietnam 64, no. 4 (2022): 60–63. http://dx.doi.org/10.31276/vjste.64(4).60-63.

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of Coronavirus Disease 2019 (COVID-19), has rapidly spread through the entire world and has become the worst pandemic from December 2019 until now. The establishment of positive standards for molecular diagnostic testing for SARS-CoV-2 plays a critical role in development and assessment of diagnostic assays associated with the shortage of positive specimens and viral culture fluids. This study aims to establish a novel assay for in-vitro RNA fragment synthesis based on amplicons of self-priming PCR targeting enve

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Doherty, P. J., S. X. Yang, R. M. Laxer, E. D. Silverman, R. Inman, and S. Pan. "Evidence for clonal expansion of T cell receptor V gamma II+ T cells in the synovial fluid of patients with arthritis." Journal of Immunology 149, no. 1 (1992): 295–99. http://dx.doi.org/10.4049/jimmunol.149.1.295.

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Abstract We have demonstrated among synovial fluid T cells a unique profile of V gamma II sequences likely arising from clonally expanded T cells. We have determined the junctional diversity associated with each expressed V gamma family by resolving amplified fragments of cDNA into component parts on large denaturing gels. Among synovial fluid T cells we frequently find dominant fragments of a unique size clearly smaller than the dominant band observed with peripheral blood T lymphocytes. In some cases the dominant bands are 12 or 15 nucleotides smaller than the corresponding most abundant ban

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Wright, Carola, Fadia Haddad, Anqi X. Qin, and Kenneth M. Baldwin. "Analysis of myosin heavy chain mRNA expression by RT-PCR." Journal of Applied Physiology 83, no. 4 (1997): 1389–96. http://dx.doi.org/10.1152/jappl.1997.83.4.1389.

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Wright, Carola, Fadia Haddad, Anqi X. Qin, and Kenneth M. Baldwin. Analysis of myosin heavy chain mRNA expression by RT-PCR. J. Appl. Physiol. 83(4): 1389–1396, 1997.—An assay was developed for rapid and sensitive analysis of myosin heavy chain (MHC) mRNA expression in rodent skeletal muscle. Only 2 μg of total RNA were necessary for the simultaneous analysis of relative mRNA expression of six different MHC genes. We designed synthetic DNA fragments as internal standards, which contained the relevant primer sequences for the adult MHC mRNAs type I, IIa, IIx, IIb as well as the embryonic and ne

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Stermer, Angela R., Gerardo Reyes, Susan J. Hall, and Kim Boekelheide. "Small RNAs in Rat Sperm Are a Predictive and Sensitive Biomarker of Exposure to the Testicular Toxicant Ethylene Glycol Monomethyl Ether." Toxicological Sciences 169, no. 2 (2019): 399–408. http://dx.doi.org/10.1093/toxsci/kfz041.

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Abstract Testicular histology and semen parameters are considered the gold standards when determining male reproductive toxicity. Ethylene glycol monomethyl ether (EGME) is a testicular toxicant with well-described effects on histopathology and sperm parameters. To compare the predictivity and sensitivity of molecular biomarkers of testicular toxicity to the traditional endpoints, small RNAs in the sperm were analyzed by next generation RNA-sequencing (RNA-seq). Adult rats were exposed to 0, 50, 60, or 75 mg/kg EGME by oral gavage for 5 consecutive days. Testis histology, epididymal sperm moti

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