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1

Nawrot-Chorabik, Katarzyna, Małgorzata Sułkowska, Małgorzata Osmenda, Vasyl Mohytych, Ewa Surówka, and Dariusz Latowski. "The Impact of Biotic and Abiotic Stress Factors on Development of European Ash Tissue Cultures." Forests 13, no. 1 (2022): 59. http://dx.doi.org/10.3390/f13010059.

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Fraxinus excelsior L. is threatened by a variety of environmental factors causing a decline of the species. The most important biotic factors negatively affecting the condition of the F. excelsior population are fungi such as the pathogen Hymenoscyphus fraxineus. Abiotic factors with potentially harmful effect to the F. excelsior population are the accumulation of heavy metals and salinity in soils. Thus, the aim of this study was to investigate the impact of selected biotic and abiotic stress factors to determine which of them pose a threat to European ash. The study was conducted using in vi
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2

Milenkovi´c, Ivan, Nenad Keča, Dragan Karadži´c, et al. "Interaction between Hymenoscyphus fraxineus and Phytophthora species on young Fraxinus excelsior seedlings." Forestry Chronicle 94, no. 02 (2018): 135–39. http://dx.doi.org/10.5558/tfc2018-020.

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Rehanek, Marius, Rim Al Kubrusli, Kira Köpke, Susanne von Bargen, and Carmen Büttner. "Detection of Viruses in Special Stands of Common Ash Reveals Insights into the Virome of Fraxinus excelsior." Forests 15, no. 8 (2024): 1379. http://dx.doi.org/10.3390/f15081379.

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Plant diseases are mostly multicausal with several factors influencing the health status of affected hosts. Common ash (Fraxinus excelsior), a significant tree species of European forests, is currently mostly endangered by ash dieback, caused by the invasive fungus Hymenoscyphus fraxineus. However, contributing factors, including pathogenic viruses, are poorly understood. Here, we report the results of a virus screening conducted on selected special stands of F. excelsior. Over three consecutive years, ash trees from different origins were tested, including leaf material from mature seed trees
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4

Selahvarzi, Bahareh, and Seyed Mohsen Hosseini. "Survival, growth and mineral accumulation in ash Fraxinus excelsior L. seedlings irrigated with water treatment effluent." Folia Forestalia Polonica, Series A - Forestry 54(3) (September 1, 2012): 159–68. https://doi.org/10.5281/zenodo.30841.

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A pot experiment was carried out to study the effect of irrigation with water treatment effluent on the growth and chemical constituents of ash seedlings Fraxinus excelsior L. according as soil chemical properties. This research was conducted at the site near to the Eastern Tehran water treatment plant. Ash seedling were planted in pots in three score rows After measuring their primary dimensions, the seedlings were irrigated with water treatment effluent, well water and water mix (50% well water + 50% water treatment effluent) for the period of April–October 2010. Three replications wer
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Keča, Nenad, Milosz Tkaczyk, Anna Żółciak, et al. "Survival of European Ash Seedlings Treated with Phosphite after Infection with the Hymenoscyphus fraxineus and Phytophthora Species." Forests 9, no. 8 (2018): 442. http://dx.doi.org/10.3390/f9080442.

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The European Fraxinus species are threatened by the alien invasive pathogen Hymenoscyphus fraxineus, which was introduced into Poland in the 1990s and has spread throughout the European continent, causing a large-scale decline of ash. There are no effective treatments to protect ash trees against ash dieback, which is caused by this pathogen, showing high variations in susceptibility at the individual level. Earlier studies have shown that the application of phosphites could improve the health of treated seedlings after artificial inoculation with H. fraxineus. Three-year-old F. excelsior seed
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Pacia, Artur, Stanisław Drozdowski, Tadeusz Malewski, et al. "Use of Phosphite Preparations to Protect Ash Seedlings Fraxinus excelsior L. against Phytophthora spp. and Hymenoscyphus fraxineus Pathogens." Forests 15, no. 5 (2024): 829. http://dx.doi.org/10.3390/f15050829.

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In this study, greenhouse tests were conducted on 240 Fraxinus excelsior seedlings to investigate the simultaneous damage caused by thea pathogenic fungus and oomycetes. The experiment was performed under controlled conditions in the greenhouse of the Institute of Forest Research in Sękocin Stary (Poland). Three species of oomycetes were used for the experiment: Phytophthora plurivora, Phytophthora taxon hungarica, Phytophthora megasperma, and the fungus Hymenoscyphus fraxineus. Inoculations using the fungus were carried out on shoots and in plant pots in which the soil was mixed with the thre
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7

Kowalski, Tadeusz, and Agata Łukomska. "The studies on ash dying (Fraxinus excelsior L.) in the Włoszczowa Forest Unit stands." Acta Agrobotanica 58, no. 2 (2012): 429–40. http://dx.doi.org/10.5586/aa.2005.068.

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The studies were carried out in the Włoszczowa Forest Unit, in 9 ash stands differing in respect of age, origin (natural, artificial), site and in the nursery on 3 quarters differing due to a silvicultural method (transplanted and not transplanted) and seedlings age. In each stand an analysis of disease symptoms was carried out on 100 trees (2 - 20 years old stands) or 50 trees (21 - 80 years old stands) growing side by side in central part of the stand, while in the nursery in each block 200 seedlings were analyzed (4 sectors with 50 seedlings each). From the infected seedlings and trees 120
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8

Bulíř, P. "Effects of varying doses of Frisol on European ash (Fraxinus excelsior L.) planted on spoil banks." Journal of Forest Science 53, No. 1 (2008): 35–40. http://dx.doi.org/10.17221/2080-jfs.

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Frisol forte (FR F) and Frisol active (FR A) are slow-acting organic fertilisers recommended for reclamation and revitalisation of undeveloped soils and degraded areas. Tentative results of experiments carried out with these products on spoil banks in the CR showed controversial reactions of plants - on the one hand a high mortality of seedlings and a very good growth on the other. Reasons for these disproportions projecting themselves into the welfare of woody plants probably lie in an incorrect dosage of products. Thus, a special long-term experiment was established with the objective to fin
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9

Vemić, Aleksandar, and Zlatan Radulović. "The effect of temperature on the Fusarium sambucinum growth from the one-year-old Fraxinus excelsior seedlings in Montenegro." REFORESTA, no. 11 (June 30, 2021): 36–44. http://dx.doi.org/10.21750/refor.11.04.92.

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Species complex Fusarium sambucinum was frequently isolated from ash stands in Montenegro. Previous researches revealed that it is dangerous pathogen for one-year-old seedlings. The aim of this research was to investigate the influence of temeperature on growth and morphological characteristics of morphotype isolated from one-year-old common ash (Fraxinus excelsior L.) seedlings in Montenegro. Growth of Fusarium sambucinum pure cultures on different temperatures was the fastest on 25°C while the absence of growth occurred on 32.5°C. Cultures on optimum temperature were white reddish, aerial an
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10

Striganavičiūtė, Greta, Jonas Žiauka, Vaida Sirgedaitė-Šėžienė, and Dorotėja Vaitiekūnaitė. "Priming of Resistance-Related Phenolics: A Study of Plant-Associated Bacteria and Hymenoscyphus fraxineus." Microorganisms 9, no. 12 (2021): 2504. http://dx.doi.org/10.3390/microorganisms9122504.

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European ash (Fraxinus excelsior) is highly affected by the pathogenic fungus Hymenoscyphus fraxineus in all of Europe. Increases in plant’s secondary metabolite (SM) production is often linked tol enhanced resistance to stress, both biotic and abiotic. Moreover, plant-associated bacteria have been shown to enhance SM production in inoculated plants. Thus, our hypothesis is that bacteria may boost ash SM production, hence priming the tree’s metabolism and facilitating higher levels of resilience to H. fraxineus. We tested three different ash genotypes and used Paenibacillus sp. and Pseudomonas
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11

Bloor, Juliette M. G., Paul W. Leadley, and Laure Barthes. "Responses of Fraxinus excelsior seedlings to grass-induced above- and below-ground competition." Plant Ecology 194, no. 2 (2007): 293–304. http://dx.doi.org/10.1007/s11258-007-9292-y.

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12

Nawrot-Chorabik, Katarzyna, Natalia Marcol-Rumak, and Dariusz Latowski. "Investigation of the Biocontrol Potential of Two Ash Endophytes against Hymenoscyphus fraxineus Using In Vitro Plant–Fungus Dual Cultures." Forests 12, no. 12 (2021): 1750. http://dx.doi.org/10.3390/f12121750.

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Development of effective biocontrol procedures using ash endophytes to combat an ash pathogen Hymenoscyphus fraxineus would be an appropriate contribution to the ongoing effort to protect European ash stands against ash decline. In this study we investigated the biocontrol potential of two ash endophytes, Thielavia basicola and Minimidochium sp., against H. fraxineus using in vitro plant-fungus and fungus-fungus dual cultures approach in three biocontrol models. The tests aimed to determine whether the endophytes show antagonism toward Fraxinus excelsior and F. pennsylvanica, to assess the lev
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13

Linaldeddu, Benedetto T., Francesco Bottecchia, Carlo Bregant, Lucia Maddau, and Lucio Montecchio. "Diplodia fraxini and Diplodia subglobosa: The Main Species Associated with Cankers and Dieback of Fraxinus excelsior in North-Eastern Italy." Forests 11, no. 8 (2020): 883. http://dx.doi.org/10.3390/f11080883.

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In Italy, after the first report in Friuli-Venezia Giulia along the border with Slovenia in 2009, ash dieback has successively been reported in Veneto, Tuscany and Trentino-Alto Adige. Given its alarming expansion in European ash formations along the sub-montane belt of north-eastern Italy and the limited information about the associated fungal microorganisms; since 2017, a study has been conducted in order to isolate and characterize the fungal species involved in the aetiology of the disease. The surveys were conducted in six ash–maple forests distributed along the Veneto and Friuli-Venezia
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14

Collin, P. "Growth Responses of Common Ash Seedlings (Fraxinus excelsior L.) to Total and Partial Defoliation." Annals of Botany 85, no. 3 (2000): 317–23. http://dx.doi.org/10.1006/anbo.1999.1045.

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15

Willoughby, Ian, Fiona L. Dixon, and David V. Clay. "Dormant season vegetation management in broadleaved transplants and direct sown ash (Fraxinus excelsior L.) seedlings." Forest Ecology and Management 222, no. 1-3 (2006): 418–26. http://dx.doi.org/10.1016/j.foreco.2005.10.048.

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16

Coello, Jaime, Míriam Piqué, Pere Rovira, Carla Fuentes, and Aitor Ameztegui. "Combining innovative mulches and soil conditioners in mountain afforestation with ash (Fraxinus excelsior L.) in the Pyrenees (NE Spain)." Forest Systems 27, no. 3 (2018): e017. http://dx.doi.org/10.5424/fs/2018273-13540.

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Aim of study: To assess the effectiveness for improving early seedling performance of the individual and combined application of (i) various doses of an innovative soil conditioner including polyacrylamide-free super-absorbent polymers, fertilizers, root precursors and humic acids; and (ii) innovative mulches based on renewable-biodegradable or recycled raw materials. The assessment was carried out in comparison with reference (commercial) soil conditioners and mulches.Area of study: Upper montane afforestation site located at 1,430 m altitude in the southern Pyrenees (NE Spain).Material and m
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17

Salek, Harmacek, Jerabkova, Topacoglu, and Machar. "Thorny Shrubs Limit the Browsing Pressure of Large Herbivores on Tree Regeneration in Temperate Lowland Forested Landscapes." Sustainability 11, no. 13 (2019): 3578. http://dx.doi.org/10.3390/su11133578.

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Thorny shrubs are considered as an important driver in the natural development of temperate forest structures, particularly in European lowland forests. We assessed the current role of thorny shrubs in the regeneration of deciduous tree species under heavy browsing pressure in a central European temperate forested landscape. The study’s military training area offered a unique opportunity to investigate the processes in which deciduous tree seedlings grew under thorny shrubs and in the close vicinity of thorny shrubs in a landscape with a high density of large herbivores (red deer and sika deer
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18

Iusypiva, Tetiana, and Galyna Miasoid. "The Impact of Industrial Pollution with Toxic Gases on Stem Histological Parameters of Woody Plant Undergrowth under Conditions of the Southern Industrial Zone of the City of Dnipro, Ukraine." International Letters of Natural Sciences 59 (October 7, 2016): 62–71. http://dx.doi.org/10.56431/p-930grq.

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The paper examines the influence of industrial emissions of sulphur (IV) and nitrogen (IV) oxides on stem histological parameters of the autochthonous woody plant species undergrowth of Acer platanoides L. and Fraxinus excelsior L., which are natural forest edificators in the steppe zone of Ukraine. It shows that stem anatomical characteristics undergo quantitative changes in the annual undergrowth shoots of the two tree species under anthropogenic burden, with the most significant changes in the area of heavy pollution. Results indicate the decrease of such stem parameters as stem and stele d
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19

Wohlmuth, Alexandra, Franz Essl, and Berthold Heinze. "Genetic analysis of inherited reduced susceptibility of Fraxinus excelsior L. seedlings in Austria to ash dieback." Forestry: An International Journal of Forest Research 91, no. 4 (2018): 514–25. http://dx.doi.org/10.1093/forestry/cpy012.

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20

Becker, Regina, Kristina Ulrich, Undine Behrendt, Volker Schneck, and Andreas Ulrich. "Genomic Characterization of Aureimonas altamirensis C2P003—A Specific Member of the Microbiome of Fraxinus excelsior Trees Tolerant to Ash Dieback." Plants 11, no. 24 (2022): 3487. http://dx.doi.org/10.3390/plants11243487.

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Some European ash trees show tolerance towards dieback caused by the invasive pathogen Hymenoscyphus fraxineus. The microbiome of these trees harbours a range of specific bacterial groups. One of these groups belonging to the species Aureimonas altamirensis was studied in detail by genome analysis and a plant inoculation trial. The strain group was shown to be phylogenetically distinct from clinical isolates by 16S rRNA analysis and phylogenomics. Genome analysis of a representative strain C2P003 resulted in a large number of unique gene sequences in comparison to other well-studied strains of
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21

Vemić, A., V. Popović, Z. Miletić, Z. Radulović, L. Rakonjac, and A. Lučić. "Effect of cadmium (Cd) and lead (Pb) soil contamination on the development of Hymenoscyphus fraxineus on Fraxinus excelsior and F. angustifolia seedlings." iForest - Biogeosciences and Forestry 16, no. 6 (2023): 307–13. http://dx.doi.org/10.3832/ifor4322-016.

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22

Abbasi, H., MR Pourmajidian, SM Hodjati, A. Fallah, and S. Nath. "Effect of soil-applied lead on mineral contents and biomass in Acer cappadocicum, Fraxinus excelsior and Platycladus orientalis seedlings." iForest - Biogeosciences and Forestry 10, no. 4 (2017): 722–28. http://dx.doi.org/10.3832/ifor2251-010.

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23

Bergqvist, Göran, Roger Bergström, and Märtha Wallgren. "Browsing by large herbivores on Scots pine (Pinus sylvestris) seedlings in mixture with ash (Fraxinus excelsior) or silver birch (Betula pendula)." Scandinavian Journal of Forest Research 27, no. 4 (2012): 372–78. http://dx.doi.org/10.1080/02827581.2011.635155.

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24

Parvanova, Petya, Nikolina Tzvetkova, Svetla Bratanova-Doncheva, Nesho Chipev, Radka Fikova, and Evgeni Donev. "Responses of Fraxinus excelsior L. seedlings to ambient ozone exposure in urban and mountain areas based on physiological characteristics and antioxidant activity." Environmental Science: Processes & Impacts 15, no. 7 (2013): 1452. http://dx.doi.org/10.1039/c3em30614c.

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25

Hebel, I., R. Haas, and Aikaterini Dounavi. "Genetic Variation of Common Ash (Fraxinus excelsior L.) Populations From Provenance Regions in Southern Germany by Using Nuclear and Chloroplast Microsatellites." Silvae Genetica 55, no. 1-6 (2006): 38–44. http://dx.doi.org/10.1515/sg-2006-0006.

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Abstract The genetic structure of 14 populations from three ash provenance regions (Fraxinus excelsior L.) in southern Germany (Aid Infodienst, 2003) is described by analysing the variation of four nuclear and five cpDNA microsatellites. The study of the nuclear microsatellites revealed high levels of genetic diversity but low levels of genetic differentiation, suggesting a high degree of gene flow among regions and/or human interference by introducing plant material coming from different provenances. The distributions of the allele frequencies and the genetic structures at these four microsat
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26

Jochner-Oette, Susanne, Tanja Rohrer, Anna-Katharina Eisen, Selina Tönnes, and Barbara Stammel. "Influence of Forest Stand Structure and Competing Understory Vegetation on Ash Regeneration—Potential Effects of Ash Dieback." Forests 12, no. 2 (2021): 128. http://dx.doi.org/10.3390/f12020128.

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Background and Objectives: The existence of common ash (Fraxinus excelsior) in Europe is severely endangered by ash dieback. To support its future sustainability, it is essential to improve the natural ash regeneration. The main aim of this study was to investigate the influence of light conditions, conceivably influenced by stand structure/ash dieback, on ash regeneration and the competition between ash seedlings and species growing in the understory. Materials and Methods: We selected 40 plots in a riparian forest located in Bavaria, Germany. Light-related variables (Leaf Area Index, gap fra
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Madsen, Palle, and Katrine Hahn. "Natural regeneration in a beech-dominated forest managed by close-to-nature principles — a gap cutting based experiment." Canadian Journal of Forest Research 38, no. 7 (2008): 1716–29. http://dx.doi.org/10.1139/x08-026.

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European beech ( Fagus sylvatica L.) is increasingly managed by close-to-nature principles, mimicking the gap dynamics of seminatural forests. The prime aim of this study was to analyse natural regeneration reliability under favourable conditions in newly formed gaps. A total of 12 gaps were created by felling three canopy trees for each gap: six gaps in each of the two winters 1996–1997 and 1997–1998. One-half of the gaps were fenced against deer. We recorded advance regeneration density (1997), regeneration density and height (1997–2002), relative light intensity (1997–2002), and volumetric
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28

Ogris, N., T. Hauptman, D. Jurc, V. Floreancig, F. Marsich, and L. Montecchio. "First Report of Chalara fraxinea on Common Ash in Italy." Plant Disease 94, no. 1 (2010): 133. http://dx.doi.org/10.1094/pdis-94-1-0133a.

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In many European countries, the anamorphic Chalara fraxinea Kowalski (teleomorph Hymenoscyphus albidus [Roberge ex Desm.] Phillips; 1–3) is responsible for a severe and rapidly spreading dieback of common ash (Fraxinus excelsior L.) since it was first reported in Poland. Recently, this disease was added to the EPPO Alert List and the NAPPO Phytosanitary Alert System. Symptomatic trees were observed in a 1.8-ha ash-maple forest in northeastern Italy (Fusine, UD; 46°30′N, 13°37′E; 782 m above sea level) along the Italo-Slovenian border in July 2009. Symptoms were found on approximately 10% of ma
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Raquininsta, Christian, Bernard Jung-Muller, Jean Dufour, and Nathalie Frascaria-Lacoste. "Rapid seedling obtaining from European ash species Fraxinus excelsior (L.) and Fraxinus angustifolia (Vahl.)." Annals of Forest Science 59, no. 2 (2002): 219–24. http://dx.doi.org/10.1051/forest:2002009.

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30

Bulíř, P. "Impact of soil conditioners on the growth of European ash (Fraxinus excelsiorL.) on dumps." Journal of Forest Science 51, No. 9 (2012): 392–402. http://dx.doi.org/10.17221/4574-jfs.

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The effects of soil conditioners TerraCottem, Frisol, Bio-algeen and fertilisers Silvamix Forte and Cererit on the survival rate and growth of European ash seedlings until the stage called established plantation were observed on two experimental plots with different anthropogenic substrates. Eight-year experiments showed that under the given conditions the tested agents fulfilled the declared effects only partially. The application of TerraCottem was statistically insignificant both in the survival rate and in the growth. In the applied dose, Frisol manifested itself as an agent that may cause
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Koveshnikov, Alexey, Nina Shiryaeva, Vladimir Naumkin, Karina Bulgakova, and Zhanna Silaeva. "The art of arboplastics in the landscape architecture." E3S Web of Conferences 175 (2020): 06004. http://dx.doi.org/10.1051/e3sconf/202017506004.

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Arbosculpture in Russia is a developing art form. Nowadays there is a small number of works devoted to this topic in our country. In this regard, the aim of the following work is to study the technology of creating arbosculptures in the conditions of the central region of Russia. The material for creating arbosculptures are woody plants of the following species: Fraxinus excelsior L., Acer platanoides L., Prunus cerasifera Ehrh, Prunus cerasus L. The formation of sculptures takes 5 years or more. The process of the creation includes such stages as – making a sketch design, selection of a breed
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Abbasi, Hooman, Mohammad Reza Pourmajidian, Seyed mohammad hodjati, and Asghar Fallah. "Comparison of Lead Uptake by Four Seedling Species (Acer cappadocicum, Fraxinus excelsior, Thuja orientalis and Cupressus arizonica)." Ecopersia 4, no. 4 (2016): 1617–29. http://dx.doi.org/10.18869/modares.ecopersia.4.4.1617.

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Frye, Jonathan, and Wolfgang Grosse. "Growth Responses to Flooding and Recovery of Deciduous Trees." Zeitschrift für Naturforschung C 47, no. 9-10 (1992): 683–89. http://dx.doi.org/10.1515/znc-1992-9-1008.

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Tree seedling flooding tolerance for 22 species was assessed under controlled field conditions. Initial heights under control (freely draining soil, n = 20 per species) and flooded (standing water, depth = 10 cm, n = 20 per species) conditions were measured in March 1990. Survival, height and diameter growth were determined after 120 days. Recovery from flooding effects was assessed in the following growing season from March to August, 1991. Taxodium distichum (L.) L. C. Rich, exhibited enhanced growth when flooded. Acer saccharinum L., Fraxinus excelsior L., and Quercus robur L. increased dia
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Mwase, Weston F., Peter S. Savill, and Gabriel Hemery. "Genetic parameter estimates for growth and form traits in common ash (Fraxinus excelsior, L.) in a breeding seedling orchard at Little Wittenham in England." New Forests 36, no. 3 (2008): 225–38. http://dx.doi.org/10.1007/s11056-008-9095-6.

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35

Jinks, Richard L., Ian Willoughby, and Corinne Baker. "Direct seeding of ash (Fraxinus excelsior L.) and sycamore (Acer pseudoplatanus L.): The effects of sowing date, pre-emergent herbicides, cultivation, and protection on seedling emergence and survival." Forest Ecology and Management 237, no. 1-3 (2006): 373–86. http://dx.doi.org/10.1016/j.foreco.2006.09.060.

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36

Mrazkova, M., K. Cerny, M. Tomsovsky, et al. "First Report of Root Rot of Pedunculate Oak and Other Forest Tree Species Caused by Phytophthora plurivora in the Czech Republic." Plant Disease 94, no. 2 (2010): 272. http://dx.doi.org/10.1094/pdis-94-2-0272b.

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From 2006 to 2008, several similar Phytophthora isolates were obtained from roots of mature Quercus robur and other tree species (Acer platanoides, Fraxinus excelsior, Q. rubra, and Tilia cordata) in forests and parks in several areas in the Czech Republic. The trees were characterized by chlorotic and reduced foliage, crown dieback, and reduced root hairs. Several isolates of Phytophthora were obtained from necrotic roots of these trees and identified as Phytophthora plurivora Jung & Burgess (1). Isolated colonies grown on V8A medium were radiate to slightly chrysanthemum shaped with limi
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Vemić, Aleksandar, Sanja Jovanović, Tanja Berić, et al. "The Potential of Pseudomonas koreensis R4.45P to Suppress Hymenoscyphus fraxineus Development in Fraxinus excelsior Leaves." Forest Pathology 55, no. 2 (2025). https://doi.org/10.1111/efp.70019.

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ABSTRACTDue to the intense ash dieback in Europe, which begins with the appearance of leaf infection, this study presents the results of the research on the impact of a selected isolate Pseudomonas koreensis R4.45P on the development of Hymenoscyphus fraxineus in the rachises of Fraxinus excelsior. Preliminary in vitro testing of P. koreensis R4.45P showed a statistically significantly lower growth of H. fraxineus compared to control cultures that were not exposed to this bacterium. The results of the in planta test on F. excelsior seedlings showed a statistically significant decrease in dieba
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38

Nawrot-Chorabik, Katarzyna, Bartosz Pluciński, and Dariusz Latowski. "Indirect auxiliary organogenesis of Fraxinus excelsior L. as a tool for ash dieback control." New Forests, June 3, 2023. http://dx.doi.org/10.1007/s11056-023-09981-x.

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AbstractThe existence of European ash (Fraxinus excelsior L.) is threatened by fungus-induced ash dieback. It is essential to find effective methods to multiply ash genotypes resistant to ash dieback while preserving the genetic diversity of these tree populations. In this paper the efficient method for production of European ash seedlings using indirect auxiliary organogenesis with multi-factor analysis of its effectiveness is presented. Procedures for a dormancy breaking treatment of seeds and effective disinfection of F. excelsior primary explants, as well as appropriate composition of the
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39

Burghard, Valentin, Sonja Wende, Volker Schneck, and Andreas Ulrich. "Inoculation of Fraxinus excelsior seedlings with bacterial strains to enhance the tolerance against ash dieback." Journal of Plant Diseases and Protection 132, no. 1 (2024). https://doi.org/10.1007/s41348-024-01014-9.

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AbstractSince ash dieback has been recognized as a major threat to Fraxinus excelsior, multiple lines of research have focused on potential management to mitigate this disease. One area of focus has been the microbiome of the trees and the application of biocontrol agents (BCAs). In this study, we aimed to find suitable BCAs that can help control Hymenoscyphus fraxineus and the resulting ash dieback. Therefore, we studied the health of ash seedlings under nursery conditions via inoculation with beneficial bacterial isolates and consortia to suppress the pathogen. By using offspring of two tole
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Lust, M., and G. Zahedi. "Growth and development of ash (Fraxinus excelsior L.) seedlings on different humus types under a closed forest canopy." Silva Gandavensis 64 (October 3, 1999). http://dx.doi.org/10.21825/sg.v64i0.828.

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In Sep. 1995, in stands with ash and oak - beech, 116 two to four-year-old ash (Fraxinus excelsior L.) seedlings were selected in 12 plots in order to assess collar diameter growth, number of leaves, branches and growth flushes (episodes) per seedling, produced during the next two years. At the same time, 30 seedlings were selected to measure the root length and the root: shoot ratio. Seedlings were growing on different levels of soil fertility (humus types of typical mull, acid mull and mull moder), under a dense canopy (2.5% light intensity). The investigation reveals that, under the high sh
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Keča, Nenad, Milosz Tkaczyk, Anna Żółciak, et al. "Survival of European Ash Seedlings Treated with Phosphite after Infection with the Hymenoscyphus fraxineus and Phytophthora Species." September 4, 2018. https://doi.org/10.3390/f9080442.

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The European <em>Fraxinus</em> species are threatened by the alien invasive pathogen <em>Hymenoscyphus fraxineus,</em> which was introduced into Poland in the 1990s and has spread throughout the European continent, causing a large-scale decline of ash. There are no effective treatments to protect ash trees against ash dieback, which is caused by this pathogen, showing high variations in susceptibility at the individual level. Earlier studies have shown that the application of phosphites could improve the health of treated seedlings after artificial inoculation with <em>H. fraxineus</em>. Three
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Tabari, M., M. Lust, and J. Neirynck. "Effect of light and humus on survival and height growth of ash (Fraxinus excelsior L.) seedlings." Silva Gandavensis 63 (October 3, 1998). http://dx.doi.org/10.21825/sg.v63i0.834.

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In 1997, 300 3-month-old ash (Fraxinus excelsior L.) seedlings were removed out of the three different humus types (100 seedlings from each humus type) in the experimental forest of Aelmoeseneie (University of Ghent) with a closed canopy, in north of Belgium. Humus types were described as active mull with pH of 5.3-6.5, acid mull with pH of 4.4-5.2 and moder mull with pH of 3.8-4.3. Seedlings were transplanted in plastic pots, filled by the original soil, and placed under five levels of light intensity (2.5, 10, 18,28 and 92%), inside and outside the forest. Only with 92% light intensity seedl
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Semizer-Cuming, Devrim, Igor Jerzy Chybicki, Reiner Finkeldey, and Erik Dahl Kjær. "Gene flow and reproductive success in ash (Fraxinus excelsior L.) in the face of ash dieback: restoration and conservation." Annals of Forest Science 78, no. 1 (2021). http://dx.doi.org/10.1007/s13595-020-01025-0.

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Abstract • Key message Ash dieback decreases individual reproductive success of ash trees leaving healthy ash overrepresented as seed and pollen parents for next-generation seedlings. Substantial gene flow over hundreds of meters combined with superior fertility of healthy trees creates optimism for the species’ future in European forests. • Context Hymenoscyphus fraxineus is causing high mortality in European ash (Fraxinus excelsior L.). Due to inheritable resistance to the pathogen, natural selection is likely to act in favour of improved resistance in ash forests following natural regenerat
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Hoenicka, Hans, Susanne Bein, Marta Starczak, and Daniel Gackowski. "Exploring the interaction between aminobutyric acid and epigenetics in modulating ash dieback response in european ash (Fraxinus excelsior)." Journal of Plant Diseases and Protection, September 14, 2024. http://dx.doi.org/10.1007/s41348-024-00989-9.

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AbstractEuropean ash populations face a significant threat from the invasive fungus Hymenoscyphus fraxineus, leading to ash dieback disease. The absence of resistant individuals and restrictions on biotechnological solutions hinder the ability to combat this widespread illness in the near term. Priming strategies offer an alternative approach to boost stress tolerance not only in this scenario but also in other endangered tree species by triggering plant defense mechanisms. The non-proteinogenic amino acid β-aminobutyric acid (BABA) has demonstrated potential in enhancing resistance to diverse
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Demir, Özge, Barbara Schulz, Laura Rabsch, Michael Steinert, and Frank Surup. "Strong antagonism of an endophyte of Fraxinus excelsior towards the ash dieback pathogen, Hymenoscyphus fraxineus , is mediated by the antifungal secondary metabolite PF1140." Applied and Environmental Microbiology, May 30, 2024. http://dx.doi.org/10.1128/aem.00665-24.

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ABSTRACT Ash dieback, caused by the fungal pathogen Hymenoscyphus fraxineus (Helotiales, Ascomycota) , is threatening the existence of the European ash, Fraxineus excelsior . During our search for biological control agents for this devastating disease, endophytic fungi were isolated from healthy plant tissues and co-cultivated with H. fraxineus to assess their antagonistic potential. Among the strains screened, Penicillium cf. manginii DSM 104493 most strongly inhibited the pathogen. Initially , DSM 104493 showed promise in planta as a biocontrol agent. Inoculation of DSM 104493 into axenicall
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Haupt, Katharina S., Katharina Mausolf, Jane Lassen, et al. "Ash seedlings in a reciprocal transplant experiment—the extent of damage of mature forest stands affects ash offspring performance." Frontiers in Forests and Global Change 7 (April 22, 2024). http://dx.doi.org/10.3389/ffgc.2024.1355098.

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In past decades, ash dieback has caused a rapid decline of European ash (Fraxinus excelsior) in temperate forests of Europe. Numerous studies focus on mitigating the negative impacts of ash dieback to forest ecosystems or identifying resistant genotypes. The role of natural selection toward genotypes withstanding ash dieback for ash regeneration has been less frequently studied with experimental means to date. This is, however, necessary in times of global change, because the preservation of ash in Europe’s forests will depend, above all, on the adaptability of the future generations of ash tr
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Dorado, F. Javier, Iryna Matsiakh, Álvaro Camisón, et al. "Methyl jasmonate spray for the protection of broad-leaf trees against oomycete and fungal pathogens." Journal of Plant Diseases and Protection 132, no. 2 (2025). https://doi.org/10.1007/s41348-025-01061-w.

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Abstract Methyl jasmonate (MeJA) is a volatile hormone produced by plants in response to stress. Exogenous application of MeJA enhances resistance to pathogens in conifers, but little is known if resistance is also enhanced in broad-leaf trees. This article reports on five independent experiments carried out to determine the preventive and curative effects of MeJA spray in broad-leaf trees in response to relevant pathogens. In Castanea sativa seedlings, preventive MeJA spray at 1 mM was the most effective dose against Phytophthora cinnamomi, and protection lasted one year. For Quercus ilex and
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A., Rigueiro-Rodríguez, and Ferreiro-Domínguez and M. R. Mosquera-Losada N. "The effects of fertilization with anaerobic, composted and pelletized sewage sludge on soil, tree growth, pasture production and biodiversity in a silvopastoral system under ash (Fraxinus excelsior L.)." March 2, 2010. https://doi.org/10.5281/zenodo.2629952.

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In silvopastoral systems, tree growth and the composition and productivity of pasture can be modified by management practices such as initial fertilization when tree seedlings are more sensitive to understorey competition. The aim of this study was to compare the effects of fertilization with different types of sewage sludge (anaerobic sludge, composted sludge and pelletized sludge), using different rates of incorporation and mineralization with traditional treatments (with and without mineral fertilizers) on the growth of newly established ash (Fraxinus excelsior L.) and on pasture developmen
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Tabari, M., N. Lust, and L. Nachtergale. "Regeneration dynamics in an alluvial dense ash (Fraxinus excelsior L.) stand." Silva Gandavensis 66 (October 3, 2001). http://dx.doi.org/10.21825/sg.v66i0.818.

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Broadleaves regeneration dynamics and the succession mechanism were studied within a transect of 14 m x 56 m in a dense 80-year-old ash stand situated on an alluvial soil. For this purpose, abundance and height of all naturally regenerated species at different development stages were analyzed and their distribution over the juvenile and older growth stages determined. The study reveals that from the main broadleaved tree species, Quercus robur and Fagus sylvatica regeneration scarcely occur at any growth stages. No Fraxinus excelsior regeneration, except 1 unit, taller than sapling (1.5-4 m) c
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Long, Feng, James M. Doonan, Lene R. Nielsen, Erik D. Kjær, and Chatchai Kosawang. "Mother trees of common ash (Fraxinus excelsior) disperse different sets of mycobiome through their seed wings." BMC Research Notes 17, no. 1 (2024). http://dx.doi.org/10.1186/s13104-024-06863-z.

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Abstract Objective The endophytic mycobiome is present in all studied plant compartments, including fruits and seeds. Here, we studied the mycobiome of seed wings as they are transferred with seeds in common ash and tested whether the mycobiome differs among trees. To achieve this, we used ITS1-based amplicon sequencing and two genotypes of F. excelsior as a model to compare the mycobiome of mother trees and their wings. Results We compared the mycobiome of 57 seed wings to the seed stalks (57) collected from two genotypes of F. excelsior using three ramets of each genotype. Alpha diversity in
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