Journal articles on the topic 'Freeh, Louis J'

THE NEWS FROM AMERICA IS (WHAT ELSE COULD IT BE?) BILL Clinton – America's first first-name-only-please President, informality and accessibility being hallmarks of democratic populism in the 1990s. It might seem as if this is the roller-coaster presidency: if you do not like Clinton's bad (good) reputation today, just wait a month and you can be sure that things will have turned upside down. When I started this piece in the spring, he was way, way down; today just a few months later, following a successful Japanese trip (his weak rivals in the G-7 group made him look good), his two successful judicial appointments (Ruth Bader Ginsberg to the Supreme Court and Louis J. Freeh to the FBI), his paper-thin but indispensable budget victory in the Congress, and his shepherding of the historic Israeli-Palestinian peace protocol, he's looking good. By the time you read this, however, he's likely to be down again, or perhaps down but once again up. His political career has been on a rollercoaster from the start and the media seem determined to keep him and the country rocking — and rolling.

UV irradiation is an essential factor in natural and artificial climate in modern environmental conditions, which has a constant effect on living systems. Collagenase, bromelain, ficin, papain (Sigma-Aldrich: St. Louis, MO, USA) and trypsin (MP biomedicals: Santa Ana, CA, USA) were the objects of this study. The substrate for hydrolysis was BSA (Sigma-Aldrich: St. Louis, MO, USA), the carriers for immobilization were chitosans (<100, 200 and 350 kDa) and chitosan succinate (Bioprogress: Shchyolkovo, Russia). The protease immobilization was carried out by the adsorption. The determination of the protein amount in samples and their catalytic activity was carried out by the modified Lowry method. UV irradiation of proteases was performed using doses 151–6040 J/m2. By the degree of photosensitivity, hydrolases can be arranged in the next row: collagenase → bromelain → ficin → papain → trypsin. Adsorption on a chitosan and succinate of chitosan leads to an increase in the stability to ultraviolet light of heterogeneous (immobilized) biocatalysts compared to free enzymes. Photoprotective effect of the chitosan may be due to the following reasons: enzyme interact with the chitosan to form photo resistant complexes; сhitosan screens active free-radicals, preventing the photooxidation of a certain number of amino acids, including the active centers of the studied enzymes under the influence of UV irradiation.

Molnár M, Hertelendy F. Signal transduction in rat myometrial cells: comparison of the actions of endothelin-1, oxytocin and prostaglandin F2α. Eur J Endocrinol 1995;133:467–74. ISSN 0804–4643 The objectives of this study were to evaluate and compare the actions of endothelin-1 (ET-1), oxytocin, prostaglandin F2α (PGF2α) and inositol 1,4,5-trisphosphate (IP3) on Ca2+ mobilization in permeabilized rat myometrial cells and to examine the activation of the inositol lipid cycle in intact myocytes. Cells were isolated from late pregnant rat myometrium and used as confluent monolayers after a single passage. All four agonists caused a biphasic release of45Ca2+ from non-mitochondrial pool(s), with the rank order of potency: oxytocin > PGF2α > ET-1 > IP3. Inhibitors of phospholipase C blocked ET-1-and oxytocin-promoted but not PGF2α-promoted 45Ca2+ efflux. Similarly, heparin, an IP3 receptor blocker, failed to inhibit PGF2α-induced Ca2+ release while inhibiting the action of the other agonists. Endothelin-1 and oxytocin stimulated inositol phosphate accumulation at concentrations similar to those that promoted 45Ca2+ efflux, whereas about 100 times higher concentrations of PGF2α were needed to activate this signaling pathway in intact cells. It is concluded that the primary action of PGF2α in myometrial cells is to enhance Ca2+ influx, whereas oxytocin and ET-1 receptors are coupled to phospholipase C, generating IP3 and raising the intracellular concentration of free Ca2+ from intracellular as well as extracellular sources. Frank Hertelendy, Dept. Ob/Gyn, St Loufis University Health Sciences Center, 3635 Vista Ave at Grand Blvd, St Louis, MO 63110-0250, USA

ABSTRACT J. Louis Martyn was not anti-Judaic but a lover of Jews and Judaism. But his passion for Paul, especially for Galatians, transcended his love for Judaism and may have caused him to downplay some of the difficulties in that letter. This article focuses on Martyn’s exegesis of the Sarah/Hagar allegory in Gal 4:21–5:1, which interprets the opposition not as Christianity versus Judaism but as the Torah-free Christian mission to Gentiles versus the Torah-observant mission—an interpretation that this article finds indefensible. The article concludes with some reflections on Martyn’s attitude toward Rom 9–11.

In several studies of Galatians, J. Louis Martyn has argued that in the allegory of Hagar and Sarah (4.1–5.1), the ‘two covenants’ of 4.24b, traditionally identified with Judaism and Christianity respectively, refer, on the one hand, to a Christian Jewish Law-observant Gentile mission, Teachers from whom are disturbing Paul's Galatian converts, and to the Law-free Gentile mission promulgated by Paul, on the other. In the light, particularly, of Paul's overall usage of ‘covenant’, Martyn's interpretation is not sustainable – though this need not imply a return to an anti-Jewish interpretation of the text.

J. Louis Martyn has identified perhaps the central question of Galatians: what time is it? This essay sheds fresh light on that question, however, by asking a corollary one: where are the Galatians? For Paul, it is argued, the Galatians are in the wilderness, somewhere between an Exodus-like redemption and the inheritance of the ‘kingdom of God’ (5.21). Paul utilises this narrative location as part of his rhetorical strategy to redress a developing crisis within his churches. The Galatians are on the verge of a wilderness apostasy, hence Paul colours his rebukes and warnings with language that evokes Israel's own tragic wilderness failings.

Mason instructional faculty have the opportunity to use a Gallup self-discovery assessment, StrengthsFinder, free to Mason faculty, staff and students, that has been proven to enhance student engagement in and outside the classroom (Lopez, S. J., & Louis, M. C., 2009). The strengths-based educational approach and applications can be applied universally across all disciplines and fields, enhancing students’ learning and engagement. Once a student understands their top 5 talents, instructors can create and use a wide variety of activities and assignments to engage the students in reflective learning. This interactive session will feature best practices using strengths-based approaches and the StrengthsFinder assessment from instructional faculty in various academic colleges. Participants will explore the various ways they can integrate strengths in academic courses.

Jean-Louis Loday and María O. Ronco introduced the notion of a trioid and constructed a free trioid of rank 1. In this paper, we give a survey of results on trioids. Different examples of trioids and new trioid theoretical constructions are given, combinatorial properties of operations on trioids are studied and some congruences on trioids are characterized. We also introduce the notion of a triband of subtrioids and in terms of tribands of subtrioids describe the structure of trioids. Moreover, we describe a free trioid of an arbitrary rank and for this trioid give an isomorphic construction. In addition, we introduce and study the notion of a semiretraction of a trioid and give some applications of semiretractions to the study of congruences on trioids. The main references are the papers by J.-L. Loday and M. O. Ronco as well as the author.

Electrode and Electrolyte Materials for Thin Film Microbatteries, Aaron O’Donoghue, Louise McGrath, Ian Povey and James Rohan In this work we assess the electrochemical performance of electrodeposited, binder-free V2O5 thin films with Li metal anodes in liquid and polymer gel electrolytes based on a pyrrolidinium based ionic liquid (C4mpyrTFSI). Coulombic efficiencies >99%, with electrode capacities >120 mAh g-1 are obtained in cyclic voltammetry analysis of crystalline V2O5 with C4mpyr-based Ionic liquid (IL) electrolytes. A polymer gel electrolyte suited to layer by layer microbattery fabrication was synthesised and tested with the crystalline V2O5 and gave large electrode capacities at a relatively high rate (110 mAh g-1 at 2 C) similar to those obtained in liquid electrolytes. Stable long-term cycling (up to 400 cycles) is obtained with Li metal anodes without capacity fade or short circuits developing in either the VC containing IL or the polymer gel analogue. In addition, no V2O5 deterioration is observed with the polymer gel electrolyte as the capacity recovered to ~125 mAh g-1 after cycling at 5 C. Furthermore, we assess surface modification of V2O5 by depositing 3nm Al2O3 via ALD which has been shown to improve the charge-discharge kinetics of oxide electrode materials. Coulombic efficiencies >99%, with peak electrode capacities >120 mAh g-1 are obtained and long-term cycling stabilities up to 625 cycles. 1) L. M. McGrath and J. F. Rohan, Batteries & Supercaps, 4 (2021) 485 – 492, High rate lithium ion cycling in electrodeposited binder-free thin film vanadium oxide cathodes with lithium metal anodes in ionic liquid and polymer gel analogue electrolytes. 2) L. M. McGrath and J. F. Rohan, Molecules, (2020), 25. 6002, Pyrrolidinium containing ionic liquid electrolytes for Li-based batteries. 3) T. Teranishi, Y. Yoshikawa, M. Yoneda, A. Kishimoto, J. Halpin, S. O’Brien, M. Modreanu and I. M. Povey, (2018), ACS Applied Energy Materials, 1(7), pp. 3277-3282, Aluminum Interdiffusion into LiCoO2 Using Atomic Layer Deposition for High Rate Lithium Ion Batteries. Figure 1: Charge/Discharge capacity of 3nm Al2O3 coated V2O5 vs Li foil in 1M LiPF6 in EC:DEC (1:1) Figure 1

-Richard Price, C.G.A. Oldendorp, C.G.A. Oldendorp's history of the Mission of the Evangelical Brethren on the Caribbean Islands of St. Thomas, St. Croix, and St. John. Edited by Johann Jakob Bossard. English edition and translation by Arnold R. Highfield and Vladimir Barac. Ann Arbor MI: Karoma, 1987. xxxv + 737 pp.-Peter J. Wilson, Lawrence E. Fisher, Colonial madness: mental health in the Barbadian social order. New Brunswick, N.J.: Rutgers University Press, 1985. xvi + 215 pp.-George N. Cave, R.B. le Page ,Acts of identity: Creloe-based approaches to language and ethnicity. Cambridge: Cambridge University Press, 1985. x + 275 pp., Andree Tabouret-Keller (eds)-H. Hoetink, Julia G. Crane, Saba silhouettes: life stories from a Caribbean island. Julia G. Crane (ed), New York: Vantage Press, 1987. x + 515 pp.-Sue N. Greene, Anne Walmsley ,Facing the sea: a new anthology from the Caribbean region. London and Kingston: Heinemann, 1986. ix + 151 pp., Nick Caistor, 190 (eds)-Melvin B. Rahming, Mark McWatt, West Indian literature and its social context. Cave Hill, Barbados, Department of English, 1985.-David Barry Gaspar, Rebecca J. Scott, Slave emancipation in Cuba: the transition to free labor, 1860-1899. Princeton, New Jersey: Princeton University Press, 1985. xviii + 319 pp.-Mary Butler, Louis A. Perez Jr., Cuba under the Platt agreement, 1902-1934. Pittsburgh: University of Pittsburgh Press, 1986. xvii + 410 pp.-Ana M. Rodríguez-Ward, Idsa E. Alegria Ortega, La comisión del status de Puerto Rico: su historia y significación. Río Piedras, Puerto Rico: Editorial Universitaria. 1982. ix + 214 pp.-Alain Buffon, Jean Crusol, Changer la Martinique: initiation a l'économie des Antilles. Paris: Editions Caribeennes, 1986. 96 pp.-Klaus de Albuquerque, Bonham C. Richardson, Panama money in Barbados, 1900-1920. Knoxville: University of Tennesse Press, 1985. xiv + 283 pp.-Steven R. Nachman, Marcel Fredericks ,Society and health in Guyana: the sociology of health care in a developing nation. Authors include Janet Fredericks. Durham: Carolina Academic Press, 1986. xv + 173 pp., John Lennon, Paul Mundy (eds)

Abstract Growing mammary epithelial cells as organoids in a three-dimensional (3D) cell culture environment represents a more physiological model system than conventional 2D adherent cell culture systems for studying many different aspects of mammary epithelial cell biology. We have developed MammoCult™ Organoid Growth Medium (Mouse), a serum- and phenol red-free medium for the long-term propagation of organoids from mouse mammary tissue. To initiate the cultures, mouse mammary glands are resected and enzymatically dissociated sequentially in Gentle Collagenase/Hyaluronidase, trypsin, and dispase/DNase I to generate a single-cell suspension, and 4.5 x 103 of the liberated cells are then embedded in Corning® Matrigel® and maintained in MammoCult™ Organoid Growth Medium (Mouse). Alternatively, freshly dissociated mammary cells can be expanded in adherent culture in EpiCult™ Plus Medium prior to seeding into Matrigel®. Approximately 8.4 ± 0.6% (mean ± SEM; n=10) of the freshly dissociated cells seeded in the Corning® Matrigel® will proliferate and generate organoids that are typically &gt; 200 µm in diameter and have a highly branched morphology, although organoids with a cystic morphology are also observed. Immunostaining of these branched organoids reveals that they are composed of a polarized epithelium made up of keratin (K) 8-expressing luminal cells and K5/14-expressing basal cells (n=7). Organoids upregulate transcripts for caseins (Csn1s1, Csn2), whey acidic protein (Wap), and lactoferrin (Ltf) upon stimulation with prolactin, hydrocortisone, and insulin (n=4). These multilineage organoids can be established from multiple inbred mouse strains, including C57Bl6/J, FVB, BALB/c, and CD-1. Organoids can be passaged every 10 - 14 days as small fragments at a 1:3 to 1:4 split ratio, and can be maintained for a minimum of 10 passages while still maintaining multilineage potential and a normal karyotype. These results demonstrate that MammoCult™ Organoid Growth Medium (Mouse) efficiently generates and expands mammary epithelial cell-derived organoids and offers researchers a novel tool for in vitro mammary studies. Citation Format: David Rowbotham, Samantha Rothwell, Allen C. Eaves, Sharon A. Louis, John Stingl. Expansion of mouse mammary epithelial stem cells in serum-free organoid growth medium [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 6018.

In an effort to develop methods to maximize the use of reproductive tissues in our own tissue distribution program (WaNPRC TDP) and to serve as platforms for application of ART to endangered non-human primate (NHP) species, we have examined the effects of collection and cryostorage on epididymal sperm (ES) collected from Macaca nemestrina (Mn). Fresh ES was collected by needle aspiration from the cauda epididymis and prepared by centrifugation in mHTF (Irvine Scientific, Santa Ana, CA, USA)+BSA (3mgmL−1) at 700g for 10min. The resultant pellet was resuspended in fresh mHTF+BSA and held at RT until required. ES subjected to freeze-thaw was collected from the cauda epididymis of testes provided by the WaNPRC TDP by slicing and swim-out into mHTF+BSA. ES was frozen in mTTE medium+5% glycerol (Sankai T et al., 1994 J. Reprod. Fertil. 101, 273). Frozen ES straws were removed from liquid nitrogen and thawed in air at RT for 10min. Thawed ES was centrifuged through an 80% PureSperm gradient (Spectrum Technologies, Healdsburg, CA, USA) at 700g for 15min. The resultant pellet was retrieved and washed in mHTF+BSA at 600g for 5min. For capacitation sperm were incubated in pre-equilibrated HTF+BSA containing 1.0mM caffeine and 0.1mM dbcAMP (Sigma, St. Louis, MO, USA) for 5–10min in a humidified atmosphere of 6% CO2 in air. In vitro-matured Mn oocytes collected from unstimulated ovaries were exposed to fresh or frozen-thawed ES whereas in vivo-matured Mn oocytes collected from superovulated ovaries were exposed to fresh ES in 50-μL Fertilized oocytes were cultured for 24–48h and assessed for cleavage on Day 3 post-insemination. Data were analyzed by single-factor ANOVA or t-test where appropriate and are expressed as mean±SD. Fresh Mn ES (n=4) exhibited very low motility at collection (38±10%) but recovered motility following processing (58±20%). Frozen-thawed ES (n=4) also exhibited low motility post-thaw (53±17%) and recovered after processing (66±28%). The percentage of acrosome-intact sperm was not significantly different (P&gt;0.05) for fresh ES (58.9±8.6%) compared to frozen-thawed ES (70.6±21.9%). Fresh and frozen-thawed ES fertilized in vitro-matured Mn oocytes at similar rates (fresh 68±10.3%, n=170 v. frozen-thawed 71.2±6.7%, n=90; P&gt;0.05). Cleavage rates of fertilized IVM oocytes were not significantly different (fresh 79±7.2% v. frozen-thawed 79.8±5.0%; P&gt;0.05). Fresh ES was also able to fertilize in vivo-matured Mn oocytes collected from superovulated ovaries (95.1±2.5%, n=98) and fertilized oocytes went on to cleave at a high rate (96.1±2.6%). These results suggest that fresh and frozen-thawed ES may be useful for applied ART in endangered species. This work was supported by NIH grant #RR00166 and the WaNPRC TDP.

Pérusse, Louis, Gregory Collier, Jacques Gagnon, Arthur S. Leon, D. C. Rao, James S. Skinner, Jack H. Wilmore, André Nadeau, Paul Z. Zimmet, and Claude Bouchard. Acute and chronic effects of exercise on leptin levels in humans. J. Appl. Physiol. 83(1): 5–10, 1997.—The acute (single bout of exercise) and chronic (exercise training) effects of exercise on plasma leptin were investigated in 97 sedentary adult men ( n = 51) and women ( n = 46) participating in the HERITAGE Family Study. Exercise training consisted of a standardized 20-wk endurance training program performed in the laboratory on a computer-controlled cycle ergometer. Maximal oxygen uptake, body composition assessed by hydrostatic weighing, and fasting insulin level were also measured before and after training. Pre- and posttraining blood samples were obtained before and after completion of a maximal exercise test on the cycle ergometer. Exercise training resulted in significant changes in maximal oxygen uptake (increase in both genders) and body compostion (reduction of fat mass in men and increase in fat-free mass in women). There were considerable interindividual differences in the leptin response to acute and chronic effects of exercise, some individuals showing either increase or reduction in leptin, others showing almost no change. On average, leptin levels were not acutely affected by exercise. After endurance training was completed, leptin levels decreased significantly in men (from 4.6 to 3.9 ng/ml; P = 0.004) but not in women. However, after the training-induced changes in body fat mass were accounted for, the effects of exercise training were no longer significant. Most of the variation observed in leptin levels after acute exercise or endurance training appears to be within the confidence intervals of the leptin assay. We conclude that there are no meaningful acute or chronic effects of exercise, independent of the amount of body fat, on leptin levels in humans.

In 1979, Louis Witten demonstrated that stationary axially symmetric Einstein field equations and those for static axially symmetric self-dual SU (2) gauge fields can both be reduced to the same (Ernst) equation. In this paper, we use this result as point of departure to prove the existence of the mass gap for quantum source-free Yang–Mills (Y–M) fields. The proof is facilitated by results of our recently published paper, J. Geom. Phys.59 (2009) 600–619. Since both pure gravity, the Einstein–Maxwell and pure Y–M fields are described for axially symmetric configurations by the Ernst equation classically, their quantum descriptions are likely to be interrelated. Correctness of this conjecture is successfully checked by reproducing (by different methods) results of Korotkin and Nicolai, Nucl. Phys. B475 (1996) 397–439, on dimensionally reduced quantum gravity. Consequently, numerous new results supporting the Faddeev–Skyrme (F–S)-type models are obtained. We found that the F–S-like model is best suited for description of electroweak interactions while strong interactions require extension of Witten's results to the SU(3) gauge group. Such an extension is nontrivial. It is linked with the symmetry group SU (3) × SU (2) × U (1) of the standard model. This result is quite rigid and should be taken into account in development of all grand unified theories. Also, the alternative (to the F–S-like) model emerges as by-product of such an extension. Both models are related to each other via known symmetry transformation. Both models possess gap in their excitation spectrum and are capable of producing knotted/linked configurations of gauge/gravity fields. In addition, the paper discusses relevance of the obtained results to heterotic strings and to scattering processes involving topology change. It ends with discussion about usefulness of this information for searches of Higgs boson.

Somatic cells in semen are a potential source of nuclei for nuclear transfer to produce genetically identical animals. This is especially important when an animal has died and the only viable genetic material available is frozen semen. In our previous studies, epithelial cells were cultured from fresh ovine and bovine ejaculates and blastocyst stage embryos were produced using these cells (Liu J et al. 2007 Biol. Reprod. special issue, 177; Liu J et al. 2008 Reprod. Fertil. Dev. 20, 102). However, growing cells from frozen semen can be difficult. We hypothesized that nuclei of the somatic cells in frozen semen can be used for nuclear transfer even though the cell membrane and cytoplasm are damaged during the semen freezing process. Electrical fusion or piezo assisted direct injection was applied to introduce nuclei of somatic cells isolated from frozen bovine semen (not cultured) into enucleated bovine oocytes. With electrical fusion, only 5 of the 64 (7.8%) recombined couplets fused, which is lower than our normal fusion rate of approximately 70%. Of the 5 fused embryos, one cleaved and developed to a 4-cell stage embryo. Staining with propidium iodide indicated that less than 10% of somatic cells isolated from frozen bovine semen were viable. These results suggest that it might not be practical to introduce nuclei of the cells in frozen semen into oocytes by fusion due to the high proportions of dead cells. Membranes of the cells in frozen semen were hard and difficult to break by piezo pulses or drawing in and out of the injection pipette. Therefore, whole cell intracytoplasmic injection was applied. After couplet recombination, activation was induced by applying two 0.3 kV cm–1, 55 μs direct-current pulses delivered by an Eppendorf Multiporator (Eppendorf, North America) in activation medium that was composed of 0.28 m Mannitol (Sigma-Aldrich, St. Louis, MO, USA), 0.1 mm CaCl2 (Sigma-Aldrich), and 0.1 mm MgSO4 (Sigma-Aldrich), followed by incubation in 10 μg mL–1 cycloheximide (Sigma-Aldrich) and 5 μg mL–1 cytochalasin B (Sigma-Aldrich) for 5 h in a humidified 5% CO2, 5% O2, and 90% N2 gas mixture at 38.5°C. Of the 299 recombined embryos, 82 (27.4%) either cleaved or were fragmented. Of the 82 embryos, 48 were examined and 42 (87.5%) were determined to be fragmented (contained intact donor cells). Low cleavage rates were observed in embryos produced by direct injection (4.5%), but no further embryonic development occurred. Cells cultured from fresh bovine semen were used as positive controls for whole cell intracytoplasmic injection. Of the 74 recombined embryos, 73 (98.6%) cleaved and 4 developed into blastocysts. These results highlight the difficulty of obtaining viable embryos by employing nuclear transfer and somatic cells obtained from frozen semen samples. Additional research is warranted given the potential value of this approach for recovering lost genetics.

During the 2005 tomato-growing (Lycopersicon esculentum Mill.) season, an apparent bacterial disease with cankers on the stems and bird's eye lesions on the fruit appeared in commercial fields and greenhouses in the San Quintin and San Simon areas (a 60-mile long coastal plain) near the central region of the Baja California Peninsula of Mexico. The disease was found in midseason, especially when plants were flowering, and the mature and ripe stage. Incidence ranged from 4 to 46%, which represented an important loss in field and greenhouse production. Symptomatic plants showed reddish brown cavities in the stem, discoloration, and water soaking of vascular tissue. Diseased tissues were washed with phosphate buffer and placed on semiselective Clavibacter medium (SCM) (1), and a gram-positive, nonmotile, nonspore-forming, aerobic, curved rod bacterium was consistently isolated and morphologically characterized. Twenty-eight isolates were identified as Clavibacter michiganensis subsp. michiganensis by polymerase chain reaction (PCR) technology with primers CMM5/CMM6 to amplify a fragment of approximately 6.2 kb (2). The isolates were also identified by REP-PCR (repetitive extragenic palindromic PCR) genomic fingerprinting techniques (3) with REP and BOX primer sets (4). Pathogenicity tests consisting of three replicates of 4-week-old tomato seedlings (cv. Tequila) were performed by spraying (twice, 2 days apart) inocula at 108 CFU/ml. Control seedlings were sprayed with sterile water. Inoculated plants previously covered in polyethylene bags were incubated in a growth chamber at 25°C for 48 h. Within 3 weeks, symptoms of reddish-brown cavities, water-soaked lesions, and asymmetrical wilting appeared on inoculated plants and were similar to those symptoms observed in the field. No symptoms were observed on control plants. Confirmation of the causal agent was done by culturing the bacteria on SCM and PCR analysis. Occurrence of the disease in San Quintin Valley is relevant because the disease is one of the five most serious tomato diseases in the peninsula. Moreover, the potential spread of the pathogen by tomato seedlings represents a permanent risk to other pathogen-free areas in the peninsula. Although bacterial canker has been observed in Baja California (Punta Colonet, Vicente Guerrero, San Quintin, and San Simon), to our knowledge, this is the first confirmation of C. michiganensis subsp. michiganensis in Baja, Mexico. References: (1). C. Alarcon et al. Phytopathology 88:306, 1998. (2) J. Dreier et al. Phytopathology 85:462, 1995. (3) F. J. Louws et al. Phytopathology 88:862, 1998. (4) J. Versalovic et al. Methods Mol. Cell. Biol. 5:25, 1994.

BackgroundPatients with relapsed or refractory Wilms tumor (WT) have poor prognoses with limited treatment options.1–3 Immunotherapy offers a promising alternative for targeted therapy but has been limited by immune evasion tactics.4–6 Adoptive cell therapy with patient-derived tumor-associated antigen-specific T cells (TAA-T) targeting 3 antigens (WT1, PRAME, and survivin) has the potential to overcome antigen loss. The objective of this phase I clinical trial is to determine the safety of administering TAA-T to patients with high-risk, relapsed/refractory solid tumors. Secondary objectives include determination of clinical efficacy and immunobiology following infusion.MethodsT cells expanded from patient peripheral blood are stimulated weekly with antigen presenting cells expressing an overlapping peptide library spanning the tumor antigens WT1, PRAME, and survivin. Following release testing, patients are infused with TAA-T on a dose escalation study, ranging from a dose of 1 x 107/m2 (dose level 1) to 4 x 107/m2 (dose level 3). Clinical and immunobiological studies performed post-infusion monitor for adverse effects and assess immune and disease responses.ResultsTherapy with TAA-T was shown to be safe and well-tolerated in patients with high-risk solid tumors on this dose-escalation study.7 A total of 18 patients have been infused, with WT as the predominant diagnosis, accounting for 10 patients. We elucidated a dose-response relationship, with a prolonged median time to progression for patients treated on dose level 3 (recommended dose level [RDL]) compared to those on dose level 1 and 2 combined (5.2 vs 2.8 months, respectively) (figure 1). Patients demonstrated prolonged progression-free survival (PFS) compared to therapy received just prior to TAA-T (figure 2). Best response observed was stable disease. A majority of patients demonstrated improved anti-tumor immunity as evidenced by antigen spreading (figure 3).Abstract 414 Figure 1Many patients have a prolonged time to progression after therapy with TAA-T compared to the time to progression with therapy received just prior to TAA-T. Four patients (P4, P10, P12, and P17) continue without disease progression. OS: osteosarcoma; NB: neuroblastoma; WT: Wilms tumor; STS: soft tissue sarcoma; ES: Ewing sarcoma; RMS: RhabdomyosarcomaAbstract 414 Figure 2Time to progression for patients treated on dose level 3 compared to those treated on dose level 1 and 2 combinedAbstract 414 Figure 3Antigen spreading post TAA-T in a patient with Wilms tumor. TAA: combined response to targeted TAA (WT1, PRAME, survivin)ConclusionsIn long-term follow up, the 1-year progression-free survival (PFS) remains improved for patients treated at the recommended dose level compared to the PFS observed with therapy received immediately prior to TAA-T (29% vs 15%, respectively). Three patients are long-term (28–38 months) survivors without disease progression or further therapy. This unique immunotherapeutic has been well-tolerated without life-threatening cytokine release syndrome. To enhance TAA-T activity further in vivo, we will evaluate the safety of prescribed lymphodepletion prior to TAA-T infusion and assess anti-tumor immunity. We expect that lymphodepletion will enhance T cell persistence and expansion and recruit endogenous immune response with altered kinetics.Ethics ApprovalThe study was approved by the Children’s National Hospital Institutional Review Board, approval number NCT02789228.ReferencesDome JS, Graf N, Geller JI, Fernandez CV, Mullen EA, Spreafico F, Van den Heuvel-Eibrink M, Pritchard-Jones K. Advances in Wilms Tumor Treatment and Biology: Progress Through International Collaboration. J Clin Oncol 2015;33(27):2999–3007.Mullen EA, Chi YY, Hibbitts E, Anderson JR, Steacy KJ, Geller JI, Green DM, Khanna G, Malogolowkin MH, Grundy PE and others. Impact of Surveillance Imaging Modality on Survival After Recurrence in Patients With Favorable-Histology Wilms Tumor: A Report From the Children’s Oncology Group. J Clin Oncol 2018:JCO1800076.Malogolowkin MH, Hemmer MT, Le-Rademacher J, Hale GA, Mehta PA, Smith AR, Kitko C, Abraham A, Abdel-Azim H, Dandoy C and others. Outcomes following autologous hematopoietic stem cell transplant for patients with relapsed Wilms’ tumor: a CIBMTR retrospective analysis. Bone Marrow Transplant 2017;52(11):1549–1555.Heczey A, Louis CU. Advances in chimeric antigen receptor immunotherapy for neuroblastoma. Discov Med 2013;16(90):287–94.Park JR, Digiusto DL, Slovak M, Wright C, Naranjo A, Wagner J, Meechoovet HB, Bautista C, Chang WC, Ostberg JR and others. Adoptive transfer of chimeric antigen receptor re-directed cytolytic T lymphocyte clones in patients with neuroblastoma. Mol Ther 2007;15(4):825–33.Lamers CH, Sleijfer S, van Steenbergen S, van Elzakker P, van Krimpen B, Groot C, Vulto A, den Bakker M, Oosterwijk E, Debets R and others. Treatment of metastatic renal cell carcinoma with CAIX CAR-engineered T cells: clinical evaluation and management of on-target toxicity. Mol Ther 2013;21(4):904–12.Hont AB, Cruz CR, Ulrey R, O’Brien B, Stanojevic M, Datar A, Albihani S, Saunders D, Hanajiri R, Panchapakesan K and others. Immunotherapy of relapsed and refractory solid tumors with ex vivo expanded multiantigen-associated specific cytotoxic T lymphocytes: A Phase I Study. J Clin Oncol 2019:JCO1900177.

The African Diaspora: A History Through Culture, by Patrick Manning (reviewed by Joseph C. Miller) Atlas of the Transatlantic Slave Trade, by David Eltis & David Richardson (reviewed by Ted Maris-Wolf) Abolition: A History of Slavery and Antislavery, by Seymour Drescher (reviewed by Gregory E. O’Malley) Paths to Freedom: Manumission in the Atlantic World, edited by Rosemary Brana-Shute & Randy J. Sparks (reviewed by Matthew Mason) You Are All Free: The Haitian Revolution and the Abolition of Slavery, by Jeremy D. Popkin (reviewed by Philippe R. Girard) Fighting for Honor: The History of African Martial Arts in the Atlantic World, by T .J. Desch Obi (reviewed by Flávio Gomes & Antonio Liberac Cardoso Simões Pires) Working the Diaspora: The Impact of African Labor on the Anglo-American World, 1650-1850, by Frederick C. Knight (reviewed by Walter Hawthorne) The Akan Diaspora in the Americas, by Kwasi Konadu (reviewed by Ray Kea) Tradition and the Black Atlantic: Critical Theory in the African Diaspora, by Henry Louis Gates Jr. (reviewed by Deborah A. Thomas) From Africa to Jamaica: The Making of an Atlantic Slave Society, 1775-1807, by Audra A. Diptee (reviewed by D.A. Dunkley) Elections, Violence and the Democratic Process in Jamaica 1944-2007, by Amanda Sives (reviewed by Douglas Midgett) Caciques and Cemi Idols: The Web Spun by Taino Rulers between Hispaniola and Puerto Rico, by José R. Oliver (reviewed by Brian D. Bates) The Latin American Identity and the African Diaspora: Ethnogenesis in Context, by Antonio Olliz Boyd (reviewed by Dawn F. Stinchcomb) Reconstructing Racial Identity and the African Past in the Dominican Republic, by Kimberly Eison Simmons (reviewed by Ginetta E.B. Candelario) Haiti and the Haitian Diaspora in the Wider Caribbean, edited by Philippe Zacaïr (reviewed by Catherine Benoît) Duvalier’s Ghosts: Race, Diaspora, and U.S. Imperialism in Haitian Literatures, by Jana Evans Braziel (reviewed by J. Michael Dash) Mainland Passage: The Cultural Anomaly of Puerto Rico, by Ramón E. Soto-Crespo (reviewed by Guillermo B. Irizarry) Report on the Island and Diocese of Puerto Rico (1647), by Diego de Torres y Vargas (reviewed by David A. Badillo) Land Reform in Puerto Rico: Modernizing the Colonial State, 1941-1969, by Ismael García-Colón (reviewed by Ricardo Pérez) Land: Its Occupation, Management, Use and Conceptualization. The Case of the Akawaio and Arekuna of the Upper Mazaruni District, Guyana, by Audrey J. Butt Colson (reviewed by Christopher Carrico) Caribbean Religious History: An Introduction, by Ennis B. Edmonds & Michelle A . Gonzalez (reviewed by N. Samuel Murrell) The Cross and the Machete: Native Baptists of Jamaica – Identity, Ministry and Legacy, by Devon Dick (reviewed by John W. Pulis) Swimming the Christian Atlantic: Judeoconversos, Afroiberians and Amerindians in the Seventeenth Century, by Jonathan Schorsch (reviewed by Richard L. Kagan) Kosmos und Kommunikation: Weltkonzeptionen in der südamerikanischen Sprachfamilie der Cariben, by Ernst Halbmayer (reviewed by Eithne B. Carlin) That Infernal Little Cuban Republic: The United States and the Cuban Revolution, by Lars Schoultz (reviewed by Antoni Kapcia) Voice of the Leopard: African Secret Societies and Cuba, by Ivor L. Miller (reviewed by Elizabeth Pérez) Guantánamo: A Working-Class History between Empire and Revolution, by Jana K. Lipman (reviewed by Barry Carr) Packaged Vacations: Tourism Development in the Spanish Caribbean, by Evan R. Ward (reviewed by Polly Pattullo) Afro-Greeks: Dialogues Between Anglophone Caribbean Literature and Classics in the Twentieth Century, by Emily Greenwood (reviewed by Gregson Davis) Caribbean Culture: Soundings on Kamau Brathwaite, edited by Annie Paul (reviewed by Paget Henry) Libertad en cadenas: Sacrificio, aporías y perdón en las letras cubanas, by Aída Beaupied (reviewed by Stephen Fay) The Trickster Comes West: Pan-African Influence in Early Black Diasporan Narratives, by Babacar M’baye (reviewed by Olabode Ibironke) Cheddi Jagan and the Politics of Power: British Guiana’s Struggle for Independence, by Colin A. Palmer (reviewed by Jay R. Mandle) A Language of Song: Journeys in the Musical World of the African Diaspora, by Samuel Charters (reviewed by Kenneth Bilby) Man Vibes: Masculinities in Jamaican Dancehall, by Donna P. Hope (reviewed by Eric Bindler)

Many different systems of free radical scavengers have been investigated during the last few years for in vitro culture of mammalian embryos. Melatonin is a potent reactive oxygen species scavenger and has been tested in the promotion of mouse embryo development in vitro (Ishizuka et al. 2000 J. Pin. Res. 28, 48–51). An effect of melatonin on bovine embryo development in vitro is described here. Slaughterhouse-derived oocytes were subjected to standard in vitro maturation and fertilization procedures. Presumptive zygotes randomly allocated to experimental groups were cultured for 3 days (Day 1–Day 3) in CR1aaLA medium (Papis et al. 2000 Theriogenology 54, 651–658) supplemented with two different concentrations of melatonin (10−6 M or 10−4 M; Sigma, St. Louis, MO, USA) or without melatonin (control). Culture was performed under two different gas atmospheres containing 4% CO2 and either normal (7%) or enhanced (20%) oxygen concentration (2 × 3 factorial analysis). At the end of Day 3, embryos from each treatment group, developed to at least the 4-cell stage, were collected and cultured without melatonin until Day 10 at optimum 4% CO2 and 7% O2 atmosphere. The numbers of blastocysts at Day 8 and hatching/hatched blastocysts at Day 10 were recorded. Five replicates of each treatment were performed. Blastocyst formation rates of presumptive zygotes and of Day 3, 4-cell embryos were calculated for each group. Differences between groups were analyzed using chi-square and/or Fisher's exact tests where appropriate. P < 0.05 was considered statistically significant. Out of 100, 100, and 101 presumptive zygotes cultured for the first 3 days in 7% oxygen with 10−4 M, 10−6 M, or no melatonin, 31 (31%), 40 (40%), and 44 (43.5%) developed to blastocyst stage and 25 (25%), 33 (33%), and 36 (36%) to hatching/hatched blastocyst stage, respectively. On the other hand, out of 102, 102, and 100 zygotes cultured in the same concentrations of melatonin, but under 20% of oxygen, an opposite tendency was observed, as 42 (41%), 25 (24.5%), and 32 (32%) blastocysts and 26 (25.5%), 21 (20.6%), and 25 (25%) hatching/hatched blastocysts developed, respectively. No statistical significance was reached here. However, out of 4-cell embryos put into in vitro culture after initial treatments in different melatonin concentrations, a decreased ratio of blastocyst formation was observed in the 10−4 M melatonin group (31/65, 47.7%) compared to that of the control (44/65, 67.7%; P = 0.0327) when the lower oxygen concentration was applied. However, a beneficial effect of melatonin was observed in the presence of 20% oxygen. Out of 61 embryos, 42 (68.9%) developed to the blastocyst stage after treatment in 10−4 M melatonin concentrations, vs. 32/63 (50.8%; P = 0.0458) blastocysts developed in control group. In conclusion, a beneficial or a harmful effect of melatonin on bovine embryo in vitro development was observed depending on the oxygen concentration during the treatment. Results presented seem to confirm a potent free radicals scavenging activity of melatonin in a bovine embryo culture system.

Introduction: Systemic light-chain (AL) amyloidosis results from clonal plasma cells that secrete toxic fibril-forming free light chains. Therapies directed at the plasma cell clone form the backbone of its management. Identification of cell-surface receptors on the clonal cells can provide targets for therapy. BCMA is one such cell-surface glycoprotein; it is principally expressed on plasma cells and supports their long-term survival (J Exp Med. 2004;199:91-98). Anti-BCMA immunotherapies are currently being studied in multiple myeloma (N Engl J Med. 2019;380:1726-1737). Membrane-bound BCMA (mBCMA) is also shed as a soluble form, sBCMA, due to γ-secretase activity that can be inhibited by a small molecule (GSI, LY-411575) (Nat Commun. 2015;6:7333; J Immunol. 2017;198(8):3081-3088). We report on mBCMA on the clonal plasma cells of AL patients and its modulation by GSI in vitro, and on sBCMA in the blood of AL patients and of mice xenografted with an AL cell line, demonstrating its correlations in vivo with free light chain (FLC) levels and plasma cell tumor burden. Methods: We analyzed mBCMA and sBCMA levels in marrow aspirate and peripheral blood samples from AL patients under an IRB approved protocol. We isolated mononuclear cells (MNC) from patient marrow aspirates with anti-CD138 microbeads (Miltenyi Biotec, Auburn, CA), and used the CD138-selected cells in culture with LY-411575 (Sigma Aldrich, St Louis, MO). We analyzed mBCMA expression by flow cytometry using APC conjugated anti-CD269 (BCMA) antibody (Biolegend, San Diego, CA, USA) and CD138 expression by PE-conjugated anti-CD138 antibody (Biolegend, San Diego, CA, USA), along with appropriate isotype controls. We injected 107 ALMC-1 reporter cells in the flanks of NOD scid gamma (NSG) mice to create a xenograft model of AL clonal plasma cell disease (Jackson Laboratories, Bar Harbor, ME). sBCMA in patients and mice and FLC in mice were measured by ELISA (R&D Systems, Minneapolis, MN; Bethyl lab Montgomery, TX respectively). Pearson and Spearman correlation analysis was used to examine associations of sBCMA and clinical disease parameters. Paired t-test was applied to compare BCMA expression before and after treatment with GSI. Results: Marrow and blood were obtained from 20 AL patients, 8 newly diagnosed, 4 with progression of disease, and 8 after treatment with >VGPR. Their median age was 65 years (range, 48-77) and 50% were female. Median plasma cells in the marrow aspirates and involved FLC levels were 5% (1-20%) and 33 mg/L (6.6-2220mg/L) respectively. Median mBCMA expression on CD138+ marrow MNC and sBCMA levels in plasma were 39% (4-83) and 28.5 ng/ml (6.6-100.3) respectively (Figure 1A-B). sBCMA levels correlated with bone marrow plasma cell percentage and iFLC (both p<0.001, Figure 1C-D). In culture with LY-411575, the percentages of CD138 cells positive for mBCMA increased from 85% to 100% with ALMC-1 cells and from 36% to 68% (p < 0.01) with patient CD138-selected cells while the sBCMA levels in culture supernatant decreased by over 50%. In NSG mice with ALMC-1 reporter cell xenografts, medians of luciferin-based bioluminescence FLUX (photons/s), λ FLC and sBCMA were 3.9x1010 (2.02x109-1.2x1011), 949.1 mg/L (868.8-23629.2), and 3.8 ng/ml (0.9-23.6) respectively. sBCMA levels correlated with FLC (Pearson r= 0.99, p<0.0001) and with FLUX (Pearson r=0.61, p=0.07). Conclusions: BCMA is expressed on AL plasma cells and sBCMA is detected in the blood of all AL patients. In this light chain disease, sBCMA may be useful as a marker of disease activity even in patients with low FLC. Furthermore, expression of mBCMA can be manipulated by treatment with a GSI, an approach which may be useful therapeutically in AL. These results provide the basis for applying anti-BCMA immunotherapies in clinical trials in relapsed refractory AL patients. Disclosures Comenzo: Sanofi-Aventis: Membership on an entity's Board of Directors or advisory committees; Unum: Membership on an entity's Board of Directors or advisory committees, Research Funding; Takeda: Research Funding; Caelum: Consultancy, Membership on an entity's Board of Directors or advisory committees; Janssen: Consultancy, Membership on an entity's Board of Directors or advisory committees, Research Funding; Karyopharm: Research Funding; Prothena Biosciences: Consultancy, Membership on an entity's Board of Directors or advisory committees, Research Funding; Myself: Patents & Royalties: Patent 9593332, Pending 20170008966.

8023 Background: Immunochemotherapy is standard of care for patients (pts) with previously untreated advanced stage follicular lymphoma (FL). Four-year data from the Phase III GALLIUM study (NCT01332968) have previously demonstrated an improvement in the primary endpoint of investigator-assessed progression-free survival (PFS) for obinutuzumab (GA101, G) plus chemotherapy (G-chemo) versus rituximab plus chemotherapy (R-chemo) (Townsend et al. ASH 2018). Here, we report efficacy and safety results from an updated analysis. Methods: Eligibility criteria: ≥18 years; advanced stage, previously untreated grade 1–3a FL; requiring treatment according to Groupe d’Etude des Lymphomes Folliculaires criteria. Pts were randomized 1:1 to receive G 1000mg IV (day [D] 1, 8 and 15 of Cycle 1; D1 of each subsequent cycle) or R 375mg/m2 IV (D1 of each cycle) with CHOP, CVP, or bendamustine for 6 or 8 cycles. Responders received maintenance therapy with the same monoclonal antibody every 2 months for 2 years. Results: 1202 pts (median age 59 years) were enrolled (n = 601 per treatment arm). Median duration of follow-up was 76.5 months. Pts receiving G- vs R-chemo demonstrated improved PFS (5-year PFS: hazard ratio [HR] 0.76; 95% CI: 0.62–0.92; p = 0.0043; 70.5% [95% CI: 66.4–74.1] vs 63.2% [95% CI: 59.0–67.1]). There was no notable difference in 5-year overall survival (OS), with few events in either arm (HR 0.87; 95% CI: 0.62–1.22; p = 0.41; G-chemo: 90.2% [95% CI: 87.5–92.4]; R-chemo: 89.4% [95% CI: 86.6–91.6]). Time-to-next-treatment (TTNT) was greater in the G- vs R-chemo arm (5-year TTNT rate: HR 0.72; 95% CI: 0.57–0.90; p = 0.0039; 79.7% [95% CI: 76.1–82.7] vs 72.9% [95% CI: 69.1–76.4]). Incidence of grade 3–5 adverse events was 79.3% in the G-chemo arm and 71.2% in the R-chemo arm, and consistent with those reported in the primary analysis (Marcus et al. N Engl J Med 2017). Conclusions: These data further demonstrate the clinically meaningful and durable benefit of treatment with G-chemo relative to R-chemo in previously untreated FL pts. Acknowledgement: GALLIUM was sponsored by F. Hoffmann-La Roche Ltd. Third-party medical writing assistance, under the direction of William Townsend, was provided by Louise Profit and Stephanie Lacey of Gardiner-Caldwell Communications, and was funded by F. Hoffmann-La Roche Ltd. Clinical trial information: NCT01332968 .

-Stanley L. Engerman, B.W. Higman, Slave populations of the British Caribbean, 1807-1834. Baltimore: The Johns Hopkins University Press, Johns Hopkins Studies in Atlantic History and Culture, 1984. xxxiii + 781 pp.-Susan Lowes, Gad J. Heuman, Between black and white: race, politics, and the free coloureds in Jamaica, 1792-1865. Westport CT: Greenwood Press, Contributions in Comparative Colonial Studies No. 5, 1981. 20 + 321 pp.-Anthony Payne, Lester D. Langley, The banana wars: an inner history of American empire, 1900-1934. Lexington KY: University Press of Kentucky, 1983. VIII + 255 pp.-Roger N. Buckley, David Geggus, Slavery, war and revolution: the British occupation of Saint Domingue, 1793-1798. New York: The Clarendon Press, Oxford University Press, 1982. xli + 492 pp.-Gabriel Debien, George Breathett, The Catholic Church in Haiti (1704-1785): selected letters, memoirs and documents. Chapel Hill NC: Documentary Publications, 1983. xii + 202 pp.-Alex Stepick, Michel S. Laguerre, American Odyssey: Haitians in New York City. Ithaca and London: Cornell University Press, 1984. 198 pp-Andres Serbin, H. Michael Erisman, The Caribbean challenge: U.S. policy in a volatile region. Boulder CO: Westview Press, 1984. xiii + 208 pp.-Andres Serbin, Ransford W. Palmer, Problems of development in beautiful countries: perspectives on the Caribbean. Lanham MD: The North-South Publishing Company, 1984. xvii + 91 pp.-Carl Stone, Anthony Payne, The politics of the Caribbean community 1961-79: regional integration among new states. Oxford: Manchester University Press, 1980. xi + 299 pp.-Evelyne Huber Stephens, Michael Manley, Jamaica: struggle in the periphery. London: Third World Media, in association with Writers and Readers Publishing Cooperative Society, 1982. xi + 259 pp.-Rhoda Reddock, Epica Task Force, Grenada: the peaceful revolution. Washington D.C., 1982. 132 pp.-Rhoda Reddock, W. Richard Jacobs ,Grenada: the route to revolution. Havana: Casa de Las Americas, 1979. 157 pp., Ian Jacobs (eds)-Jacqueline Anne Braveboy-Wagner, Andres Serbin, Geopolitica de las relaciones de Venezuela con el Caribe. Caracas: Fundación Fondo Editorial Acta Cientifica Venezolana, 1983.-Idsa E. Alegria-Ortega, Jorge Heine, Time for decision: the United States and Puerto Rico. Lanham MD: North-South Publishing Co., 1983. xi + 303 pp.-Richard Hart, Edward A. Alpers ,Walter Rodney, revolutionary and scholar: a tribute. Los Angeles: Center for Afro-American Studies and African Studies Center, University of California, 1982. xi + 187 pp., Pierre-Michel Fontaine (eds)-Paul Sutton, Patrick Solomon, Solomon: an autobiography. Trinidad: Inprint Caribbean, 1981. x + 253 pp.-Paul Sutton, Selwyn R. Cudjoe, Movement of the people: essays on independence. Ithaca NY: Calaloux Publications, 1983. xii + 217 pp.-David Barry Gaspar, Richard Price, To slay the Hydra: Dutch colonial perspectives on the Saramaka wars. Ann Arbor MI: Karoma Publishers, 1983. 249 pp.-Gary Brana-Shute, R. van Lier, Bonuman: een studie van zeven religieuze specialisten in Suriname. Leiden: Institute of Cultural and Social Studies, ICA Publication no. 60, 1983. iii + 132 pp.-W. van Wetering, Charles J. Wooding, Evolving culture: a cross-cultural study of Suriname, West Africa and the Caribbean. Washington: University Press of America 1981. 343 pp.-Humphrey E. Lamur, Sergio Diaz-Briquets, The health revolution in Cuba. Austin: University of Texas Press, 1983. xvii + 227 pp.-Forrest D. Colburn, Ramesh F. Ramsaran, The monetary and financial system of the Bahamas: growth, structure and operation. Mona, Jamaica: Institute of Social and Economic Research, University of the West Indies, 1984. xiii + 409 pp.-Wim Statius Muller, A.M.G. Rutten, Leven en werken van de dichter-musicus J.S. Corsen. Assen, The Netherlands: Van Gorcum, 1983. xiv + 340 pp.-Louis Allaire, Ricardo E. Alegria, Ball courts and ceremonial plazas in the West Indies. New Haven: Department of Anthropology of Yale University, Yale University Publications in Anthropology No. 79, 1983. lx + 185 pp.-Kenneth Ramchand, Sandra Paquet, The Novels of George Lamming. London: Heinemann, 1982. 132 pp.

Programmable nucleases (ZFN, Tal Effector Nucleases, and CRISPR) opened a new era for mammal genome editing, in particular for the pigs used for xenotransplantation. Multiple gene editing events are required both for knockout (KO) of xenoantigens and for targeted integration of human protective genes (Perota et al. 2016 J. Genet. Genomics 43, 233–23). The objective of the present work was to edit selected pig lines to KO the enzymes coding for the most relevant xenoantigens (i.e. GGTA1, CMAH, and B4GalNT2), combining Talens and CRISPR/Cas9 technologies to magnetic beads selection (Li et al. 2013 Xenotransplantation 22, 20–31). Primary porcine adult fibroblasts were transfected using Nucleofector (V-024 program). In a single reaction 2 × 106 fibroblasts were co-transfected using 2 different sets of TALENS (4 μg/set) specific for CMAH (Conchon et al., 2013) and GGTA1 (Perota et al., 2015) genes together with B4GalNT2-specific CRISPR/Cas9 expression vector (2 μg; pX330-B4GalNT2; Estrada et al., 2015). Eight days post-transfection (DPT), Gal–/– cells were selected initially using biotin-conjugated IB4 lectin (Sigma, St. Louis, MO, USA) and magnetic beads (Dynabeads M-280, Thermo Fisher Scientific, Waltham, MA, USA). The selected cells were then plated on 150-mm Petri dishes (200 cells/dish) and cultured for 10 days. Selected colonies were expanded for PCR analysis and cryopreserved for somatic cell nuclear transfer (SCNT). All colonies were analysed by PCR for CMAH gene and their resulting products were digested with HindIII (HindIII-RFLP). Colonies that lost wild-type HindIII as a consequence of Talens effected deletion were PCR characterised for GGTA1, selecting those that had detectable Indels after gel electrophoresis and finally analysed by PCR for B4GalNT2. All PCR products were validated by sequencing for all the 3 genes of interest (TopoTA, Thermo Fisher Scientific). Selected colonies were used as nuclear donors for SCNT (Lagutina et al., 2006). Eight DPT we obtained 3.45 ×106 cells. About 6.0 × 103 Gal-negative cells (0.17%) were collected from the supernatant after magnetic beads separation. Eighteen DPT, 120 colonies were picked up and their HindIII-RFLP analyses on CMAH gene revealed that 22 colonies (18.3%) were KO for both CMAH alleles. Of these 22 colonies following electrophoretic analyses of GGTA1-PCR products, 13 colonies had detectable Indels. These 13 colonies were finally PCR analysed and sequenced for B4GalNT2 and sequenced. Final sequencing results confirmed that 2 colonies (1.6%) resulted in KO for the 3 genes. Three different zona-free SCNT experiments were done and 579 reconstructed embryos were obtained. On Day 7, 322 morulae or blastocysts (56%) were transferred in 3 synchronised sows and 2 (66%) became pregnant. In conclusion, after gene editing with programmable nucleases, combining beads-mediated selection with well-designed molecular analyses, we developed a multistep assay that can be used efficiently to detect desired gene edited events in cell colonies suitable for the SCNT. Embryos generated after SCNT were able to establish pregnancies at a high rate. This work is supported by European FP7 grants Translink (n° 603049) and Xenoislet (n° 601827).

Globalization and the Po st-Creole Imagination: Notes on Fleeing the Plantation,by Michaeline A. Crichlow with Patricia Northover (reviewed by Raquel Romberg)Afro-Caribbean Religions: An Introduction to their Historical, Cultural, and Sacred Traditions, by Nathaniel Samuel Murrell (reviewed by James Houk) Africas of the Americas: Beyond the Search for Origins in the Study of Afro-Atlantic Religions, edited by Stephan Palmié (reviewed by Aisha Khan) Òrìṣà Devotion as World Religion: The Globalization of Yorùbá Religious Culture, edited by Jacob K. Olupona & Terry Rey (reviewed by Brian Brazeal) Sacred Spaces and Religious Traditions in Oriente Cuba, by Jualynne E. Dodson (reviewed by Kristina Wirtz) The Coolie Speaks: Chinese Indentured Laborers and African Slaves of Cuba, by Lisa Yun (reviewed by W. Look Lai) Cuba and Western Intellectuals since 1959, by Kepa Artaraz (reviewed by Anthony P. Maingot) Inside El Barrio: A Bottom-Up View of Neighborhood Life in Castro’s Cuba, by Henry Louis Taylor, Jr. (reviewed by Mona Rosendahl) On Location in Cuba: Street Filmmaking During Times of Transition, by Ann Marie Stock (reviewed by Cristina Venegas) Cuba in The Special Period: Culture and Ideology in the 1990s, edited by Ariana Hernandez-Reguant (reviewed by Myrna García-Calderón) The Cubans of Union City: Immigrants and Exiles in a New Jersey Community. Yolanda Prieto (reviewed by Jorge Duany) Target Culebra: How 743 Islanders Took On the Entire U.S. Navy and Won, by Richard D. Copaken (reviewed by Jorge Rodríguez Beruff) The World of the Haitian Revolution, edited by David Patrick Geggus & Norman Fiering (reviewed by Yvonne Fabella) Bon Papa: Haiti’s Golden Years, by Bernard Diederich (reviewed by Robert Fatton, Jr.) 1959: The Year that Inflamed the Caribbean, by Bernard Diederich (reviewed by Landon Yarrington) Dominican Cultures: The Making of a Caribbean Society, edited by Bernardo Vega (reviewed by Anthony R. Stevens-Acevedo) Chanting Down the New Jerusalem: Calypso, Christianity, and Capitalism in the Caribbean, by Francio Guadeloupe (reviewed by Catherine Benoît) Once Jews: Stories of Caribbean Sephardim, by Josette Capriles Goldish (reviewed by Aviva Ben-Ur) Black and White Sands: A Bohemian Life in the Colonial Caribbean, by Elma Napier (reviewed by Peter Hulme) West Indian Slavery and British Abolition, 1783-1807, by David Beck Ryden (reviewed by Justin Roberts) The Children of Africa in the Colonies: Free People of Color in Barbados in the Age of Emancipation, by Melanie J. Newton (reviewed by Olwyn M. Blouet) Friends and Enemies: The Scribal Politics of Post/Colonial Literature, by Chris Bongie (reviewed by Jacqueline Couti) Nationalism and the Formation of Caribbean Literature, by Leah Reade Rosenberg (reviewed by Bénédicte Ledent) Signs of Dissent: Maryse Condé and Postcolonial Criticism, by Dawn Fulton (reviewed by Florence Ramond Jurney) The Archaeology of the Caribbean, by Samuel M. Wilson (reviewed by Frederick H. Smith) Crossing the Borders: New Methods and Techniques in the Study of Archaeological Materials from the Caribbean, edited by Corinne L. Hofman, Menno L.P. Hoogland & Annelou L. van Gijn (reviewed by Mark Kostro)

Abstract Renal cell carcinomas (RCC) are characterized by their heterogenous clinical outcomes, and due to their indeterminate behavior and the absence of routine biomarkers, it is difficult to identify patients who are at high-risk for relapse after curative nephrectomy. To identify genomic biomarkers for clear cell RCC (ccRCC) risk-stratification we interrogated somatic mutation status of 12 RCC-relevant genes using next-generation sequencing (NGS) in tumor-normal pairs from 943 patients with matched follow up data from the Cancer Genomics of the Kidney (CAGEKID) study. We examined associations between genomically-defined patient groups, explained below, and disease-free as well as RCC-specific survival independently in two cohorts of patients (N=469 for cohort 1; 474 for cohort 2). We used the Kaplan-Meier method with log-rank tests to compare survival functions, and Cox proportional hazards models to stratify for patient stage and age to estimate association of each group with survival. RCC-specific survival was assessed with a competing-risks method to include deaths from other causes. Within these cohorts, 76.4% of patients harbored somatic mutations in VHL, the most common driver gene in ccRCC. The most commonly mutated genes within VHL-mutated tumors were PBRM1 (39.7%), SETD2 (19%), BAP1 (14.3%), and KDM5C (8.3%). Less frequently mutated genes included ATM, COL11A1, DMD, TP53, and TRRAP (~3-5%).Among VHL-driven tumors, we identified a new genomic classifier on the basis of the number of mutations in additional RCC driver genes in the panel examined. Patients were classified based on the presence of mutations only in VHL (VHL+0), those with mutations in VHL and one other driver gene (VHL+1), two other driver genes (VHL+2), and 3 or more other driver genes (VHL≥3). We observed within both cohorts that both the risk of disease recurrence as well as RCC-specific death were associated with an increased number of mutations within this classification. When stratified for patient stage and age, the hazard-ratio for 5-year disease-free survival for VHL≥3 patients was 6.69 (p=0.000212), 4.31 for VHL+2 (p=0.000862), and 2.43 for VHL+1 (p=0.035662), compared to patients with only mutations in VHL. These observations were replicated in the second patient cohort, with hazards ratios of 4.55, 2.49, and 1.40, for VHL≥3, VHL+2, and VHL+1 classified patients respectively, indicating that risk of disease recurrence increases with the number of driver mutations. Notably, tumor mutational burden (TMB) was not significantly different between the aforementioned groups, demonstrating that our classifier is independent of TMB. We created a model based on a set of 12 RCC-relevant genes, which can predict risk of relapse for the ~80% of patients with ccRCC that are VHL-driven. This classification can be defined based on a small panel of genes, making it easily applicable to the clinic, in the context of tumor or liquid biopsy analysis. Citation Format: Kate I. Glennon, Naveen S. Vasudev, Ghislaine Scelo, Michelle Wilson, Louis Letourneau, Robert Eveleigh, Nazanin Nourbehesht, Madeleine Arseneault, Antoine Paccard, Lars Egevad, Juris Viksna, Edgars Celms, Sharon M. Jackson, Behnoush Abedi-Ardekani, Anne Y. Warren, Peter J. Selby, Sebastian Trainor, Michael Kimuli, Naeem Soomro, Adebanji Adeyoju, Poulam Patel, Magdalena B. Wozniak, Ivana Holcatova, Antonin Brisuda, Vladimir Janout, Estelle Chanudet, David Zaridze, Anush Moukeria, Oxana Shangina, Lenka Foretova, Marie Navratilova, Dana Mates, Viorel Jinga, Ljiljana Bogdanovic, Bozidar Kovacevic, Anne Cambon-Thomsen, Guillaume Bourque, Alvis Brazma, Jörg Tost, Paul Brennan, Mark Lathrop, Yasser Riazalhosseini, Rosamonde E. Banks. Genomic classification to refine prognosis in clear cell renal cell carcinoma [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr LB113.

During the spring of 2014, a severe leaf spot disease was observed on carrot (Daucus carota), parsley (Petroselinum crispum), and parsnip (Pastinaca sativa) on a 0.5-ha vegetable farm in Vojvodina Province, Serbia. The disease appeared under wet and cool conditions with 5 to 25% of plants infected for each of the three crops. Symptoms were characterized as brown angular leaf spots, ~2 mm in diameter, often limited by veins. Collected symptomatic leaves were rinsed and dried at room temperature, and leaf sections taken from the margin of necrotic tissue were macerated in sterile phosphate buffer and streaked onto nutrient agar with 5% (w/v) sucrose (NAS). After isolation, whitish, circular, dome-shaped, Levan-positive colonies consistently formed. Five strains from each host (carrot, parsley, and parsnip) were used for further study. Strains were gram-negative, aerobic, and positive for catalase and tobacco hypersensitive reaction but negative for oxidase, rot of potato slices, and arginine dihydrolase. These reactions corresponded to LOPAT group Ia, which includes Pseudomonas syringae pathovars (3). Repetitive extragenic palindromic sequence (Rep)-PCR fingerprint profiles using the REP, ERIC, and BOX primers (4) were identical for all strains. Sequence typing of the housekeeping genes gyrB and rpoD (1) was performed for three representative strains (one from each host). Sequences were deposited in the NCBI GenBank database as accessions KM979434 to KM979436 (strains from carrot, parsnip, and parsley, respectively) for the gyrB gene and KM979437 to KM979439 (strains from parsnip, parsley and carrot, respectively) for the rpoD gene. Sequences were compared with pathotype strain Pseudomonas syringae pv. coriandricola ICMP12471 deposited in the Plant Associated and Environmental Microbes Database ( http://genome.ppws.vt.edu/cgi-bin/MLST/home.pl ). BLAST analysis revealed 100% homology for gyrB and 99% homology for rpoD. Pathogenicity was tested with five representative strains from each host on four-week-old plants of carrot (cv. Nantes), parsley (cv. NS Molski), and parsnip (cv. Dugi beli glatki) using two methods: spraying the bacterial suspension (108 CFU ml−1) on the leaves until runoff (5) and injecting the bacterial suspension into leaves with a hypodermic syringe (2). Four plants were used per strain and method. Sterile distilled water was applied as a negative control treatment for each plant species. All plants were kept in a mist room with 100% humidity for 4 h, then transferred to a greenhouse at 25°C and 80% relative humidity and examined for symptom development over a period of three weeks. For all strains, inoculated leaves first developed water-soaked lesions on the leaves 5 to 7 days after inoculation (DAI); 14 DAI lesions became dark brown, often surrounded by haloes. No symptoms were observed on control plants inoculated with sterile distilled water. For fulfillment of Koch's postulates, re-isolations were done onto NAS. Re-isolated bacteria were obtained from each inoculated host and confirmed to be identical to the original isolates using the LOPAT tests and Rep-PCR fingerprinting profiles. Based on the pathogenicity test accompanied by completion of Koch's postulates, sequence analysis, and bacteriological tests, the strains were identified as P. s. pv. coriandricola. To our knowledge, this is the first report of bacterial leaf spot of carrot, parsley, and parsnip in Serbia. It may present a threat to production due to quality requirements for fresh market. References: (1) P. Ferrente and M. Scortichini. Plant Pathol. 59:954, 2010. (2) M. Gupta et al. Plant Dis. 97:418, 2013. (3) R. A. Lelliott et al. J. Appl. Bacteriol. 29:470, 1966. (4) F. J. Louws et al. Appl. Environ. Microb. 60:2286, 1994. (5) X. Xu and S. A. Miller. Plant Dis. 97:988, 2013.

Abstract Introduction: Patients with diffuse large B-cell lymphoma (DLBCL) that is resistant to chimeric antigen receptor T-cell (CAR-T) therapy have poor outcomes (Chow VA, et al. Am J Hematol. 2019;94:E209-E13). The majority of patients with DLBCL who relapse after CAR-T therapy do so with disease that continues to express CD19 surface antigen (Shah NN, Fry TJ. Nat Rev Clin Oncol. 2019;16:372-85); however, it is unknown whether treatment with CD19-targeted agents is an effective strategy for patients with prior failure of anti-CD19 CAR-T therapy. Loncastuximab tesirine (loncastuximab tesirine-lpyl; Lonca) is an FDA-approved CD19-directed antibody-drug conjugate (ADC) which had encouraging phase 1 antitumor activity and acceptable safety in non-Hodgkin lymphoma (Hamadani M, et al. Blood. 2021;137:2634-2645). In the Phase 2 LOTIS-2 trial (NCT03589469) the efficacy and safety of Lonca was evaluated in patients with relapsed or refractory (R/R) DLBCL after ≥2 lines of systemic treatments (Caimi PF, et al. Lancet Oncol. 2021;22:790-800). The overall response rate (ORR) was 48.3%. The aim of this post-hoc analysis of the LOTIS-2 trial was to investigate the antitumor activity of Lonca in patients with DLBCL relapsed or refractory after CAR-T therapy. Methods: The methodology of the LOTIS-2 trial has been published. Briefly, patients were treated with Lonca (0.15 mg/kg for the first 2 cycles then 0.075 mg/kg for subsequent cycles) administered as a single 30-minute infusion, once every 3 weeks for up to 1 year, or until progressive disease or unacceptable toxicity. Patients with previous anti-CD19 CAR-T therapy were required to have persistent CD19 expression, evaluated by local review of immunohistochemistry of a post-CAR-T biopsy. The primary endpoint was ORR, defined as the proportion of patients with best overall response of complete response (CR) or partial response (PR), determined by independent review. Secondary endpoints included overall survival (OS), progression free survival (PFS), and duration of response (DOR). PET/CT imaging was performed 6 and 12 weeks after the first Lonca dose and every 9 weeks thereafter. Response was assessed using the Lugano 2014 criteria. Kaplan Meier survival analysis was performed from initiation of Lonca treatment. Results: The characteristics of 13 patients with DLBCL with disease relapse or progression after anti-CD19 CAR-T therapy are shown in table 1. The median time interval between CAR-T infusion and Lonca treatment was 7 months (range, 45-400 days). Ten (77%) patients received Lonca as the first therapy after CAR-T failure, 3 patients received other treatments prior to Lonca (chemoimmunotherapy [R-GemOx], n = 1; allogenic stem cell transplant, n = 1; chemoimmunotherapy [R-GemOx] followed by venetoclax + bromodomain inhibitor, n =1). The ORR to Lonca was 46.2% (n=6; CR, 15.4% [n = 2]; PR, 30.8% [n = 4]) after a median of 2 cycles (range, 1-9). Of the 6 patients who achieved a response to Lonca, 5 had a previous response to CAR-T and 1 had prolonged, stable disease for &gt;1 year after CAR-T. With a median follow-up of 8 months, the median OS and PFS were 8.2 and 1.4 months, respectively (Figure 1); the 1-year OS estimate was 33.3%. The median DOR to Lonca was 8 months. Conclusions: Lonca achieved a response in 6 out of 13 patients who had failed prior CAR-T therapy. Five out of 6 responding patients had previously presented at least a partial response after CAR-T therapy. These data suggest that in patients without CD19 antigen loss, repeat therapy with another agent targeting this antigen can result in disease control. Prior response to anti-CD19 therapy may be associated with subsequent response to a second anti-CD19 treatment. Further studies are needed to confirm the feasibility and value of repeated anti-CD19 treatments in patients with B-cell non-Hodgkin lymphoma. Funding: This study was funded by ADC Therapeutics; medical writing support was provided by CiTRUS Health Group. Figure 1 Figure 1. Disclosures Caimi: Amgen Therapeutics.: Consultancy; XaTek: Patents & Royalties: Royalties from patents (wife); ADC Theraputics: Consultancy, Research Funding; Genentech: Research Funding; Kite Pharmaceuticals: Consultancy; Verastem: Consultancy; Seattle Genetics: Consultancy; TG Therapeutics: Honoraria. Ardeshna: Gilead, Beigene, Celegene, Novartis and Roche: Honoraria; Norvartis, BMS, Autolus, ADCT, Pharmocyclics and Jansen: Research Funding; Gilead, Beigene, Celegene, Novartis and Roche: Membership on an entity's Board of Directors or advisory committees. Reid: Aptose Biosciences: Other: Serves as Principle Investigator, Research Funding; ADC Therapeutics: Other: Serves as Principle Investigator, Research Funding; Millennium Pharmaceuticals: Other: Serves as Principle Investigator, Research Funding; Xencor: Other: Serves as Principle Investigator, Research Funding. Ai: Kymria, Kite, ADC Therapeutics, BeiGene: Consultancy. Lunning: Myeloid Therapeutics: Consultancy; Spectrum: Consultancy; Daiichi-Sankyo: Consultancy; Verastem: Consultancy; Janssen: Consultancy; AstraZeneca: Consultancy; Morphosys: Consultancy; Beigene: Consultancy; Legend: Consultancy; ADC Therapeutics: Consultancy; Acrotech: Consultancy; Celgene, a Bristol Myers Squibb Co.: Consultancy; AbbVie: Consultancy; Kite, a Gilead Company: Consultancy; TG Therapeutics: Consultancy; Novartis: Consultancy; Kyowa Kirin: Consultancy; Karyopharm: Consultancy. Zain: Secura Bio, DaichiSankyo, Abbvie: Research Funding; Kiyoaw Kirin, Secura Bio, Seattle Genetics: Honoraria; Secura Bio, Ono , Legend, Kiyowa Kirin, Myeloid Therapeutics Verastem Daichi Sankyo: Consultancy. Solh: ADCT Therapeutics: Consultancy, Research Funding; Jazz Pharmaceuticals: Consultancy; BMS: Consultancy; Partner Therapeutics: Research Funding. Kahl: AbbVie, Acerta, ADCT, AstraZeneca, BeiGene, Genentech: Research Funding; AbbVie, Adaptive, ADCT, AstraZeneca, Bayer, BeiGene, Bristol-Myers Squibb, Celgene, Genentech, Incyte, Janssen, Karyopharm, Kite, MEI, Pharmacyclics, Roche, TG Therapeutics, and Teva: Consultancy. Hamadani: Takeda, Spectrum Pharmaceuticals and Astellas Pharma: Research Funding; Janssen, Incyte, ADC Therapeutics, Omeros, Morphosys, Kite: Consultancy; Sanofi, Genzyme, AstraZeneca, BeiGene: Speakers Bureau.

Годы творческого формирования А.-Л.-Т. Водуайе пришлись на 1780-е, период расцвета эпохи Просвещения. Водуайе был весьма успешен и оставался востребованным на протяжении всего сложного и долгого революционного периода. Спектр дошедших до нас проектов Водуайе чрезвычайно разнообразен, и кажется почти невозможным вычленить из них что-то, что можно назвать «творческим методом». Во многом он являлся сыном эпохи Разума, однако свои взгляды на теорию архитектуры он оформил только в 1832 г. В его «Рассуждении об архитектуре» постоянно чередуется просвещенческое и романтическое, и анализ этого текста поможет понять трансформации, происходившие с «новым классицизмом» и преемственность между XVIII и XIX вв. Примирить свои зачастую противоположные представления о зодчестве (например, идею о свободе романтического гения с идеей существования неизменных принципов архитектуры) Водуайе пытался, постоянно обращаясь к сравнению с другими «свободными искусствами». Изучение работы Водуайе показывает, что в XIX в. для поколения его учеников архитектура больше не являлась типом мышления, и Водуайе не мог говорить об архитектуре на языке эпохи Просвещения, который подразумевал, что теории зодчества являются неотъемлемой частью общественной и философской дискуссии. Ему иначе приходилось «популяризировать архитектуру», переводя ее на язык других свободных искусств (музыки, живописи, поэзии), который был понятен молодой аудитории. One of the constant challenges in studying architectural theory of the French Enlightenment is to define what belongs to it and what does not. The approaches range from a specific period in the second half of the 18th century to a more broad definition, which encompasses almost 150 years, from the 1670s until 1820. Thanks to the almost universal acceptance of Emil Kaufmann’s theory, the projects and ideas of Claude Nicolas Ledoux, Étienne-Louis Boullée and Jean-Jacques Lequeu became the main symbol of Enlightenment in architecture. However, to explore the turning point of the architectural theory of that era we need to go beyond the more traditionally studied thinkers and practitioners. Antoine-Laurent-Thomas Vaudoyer was J.-J. Lequeu’s senior by a year, and his formative years fell in the same period, the 1780s, the years when architecture of the age of Reason was at its peak. Unlike many of his contemporaries Vaudoyer managed not to fall out of grace during the long and troublesome historical period of French revolutions. Due in part to this long career, his projects that remain until today are so various in style and approach, and it’s hardly possible to find a consistent modus operandi of this architect. But we could safely argue that he was in many ways one of the sons of the Enlightenment era in his architecture. What makes him especially interesting to study is that he formulated his theoretical views on this art only in 1832, at the time when the new Romantic Movement was powerful enough for him to try and bridge the opposing tendencies of both periods in his writing. He held simultaneously, the views of the age of Reason that essential unchanging principles exist in architecture, and supported new ideas about the free genius of every artist that can easily escape the constraints of reason. The article analyses the writings of Vaudoyer in conjunction with his projects, to uncover and reconstruct his ideas about the theory of architecture. As any theoretician of the Enlightenment era, Vaudoyer thinks that architecture is derived from nature. However, where other theoreticians considered cosmic geometry, natural forms and other ‘objective’ manifestations of nature to be the foundation, Vaudoyer shifts his focus towards the perception of nature’s forms and effects by those with a talent for architecture. According to him, architectural theory helps to enhance the ‘natural talent’, and is essential to foster an understanding of the art of architecture to those who cannot practice it. Vaudoyer draws a clear distinction between the ‘art’ and the ‘science’ of architecture, while their indivisible unity was paramount for other thinkers of the Enlightenment. In this and other aspects of his theory, Vaudoyer tries to combine the approaches to architecture of Romanticism with the ideas of what architecture is and should be, which stem from his formative years during the Enlightenment. He constantly drew upon the examples of other liberal arts, more “romantic” and individualistic in some aspects, to explain his architectural theories. It this he differs greatly from classic Enlightenment architectural writings where architecture was pretty much self-explanatory. The Enlightenment popularized architecture in the sense of architectural theory permeating the social and philosophical discourse and being their intrinsic part. Analyzing Vaudoyer’s writing, we can conclude that he spoke as ‘a relic of the Enlightenment’ at the time when architecture was no longer seen as a way of thinking. Thus, for him popularizing architecture is no longer about deriving all other social, philosophical and artistic discourses from architecture, but rather interpreting architecture through the language of other liberal arts, which was much more familiar to the new generation of the 1830s.

Abstract Abstract 4065 Doxorubicin and pegylated liposomal doxorubicin have shown efficacy for the treatment of multiple myeloma (MM). Most recently, anthracyclines including doxorubicin have inhibited vascular development in tumor tissues by reducing intracellular hypoxia inhibitory factor (HIF)-1α levels. However, the efficacy of these drugs as single agents and in combination therapies is limited by their myelosuppressive, cardiac and dermatological side effects. INNO-206 (CytRx Corporation, Los Angeles, CA) is an albumin-binding prodrug of doxorubicin that is released from albumin under acidic conditions. Extracellularly, tumor tissues including MM are often acidic which allows free doxorubicin to be released from INNO-206. Following cellular uptake, it is released in the acidic endosomal or lysosomal compartments. Thus, this drug offers the possibility to achieve higher levels of active doxorubicin within tumor cells than with conventional doxorubicin. INNO-206 has not been previously evaluated using animal models in any hematologic malignancies including MM. First, we determined the effects of INNO-206 at varying pH levels on MM tumor cell proliferation and apoptosis in vitro. Second, we have developed a novel approach to test the anti-angiogenic activity of new agents in a rapid, accurate and inexpensive way using a combined chorioallantoic membrane (CAM) and feather bud (FB) assay (Chen et al. Int J Oncol 2010) and used this model to evaluate the anti-angiogenic effects of INNO-206 in a concentration- and pH-dependent fashion. Third, we determined the anti-MM effects and toxicity of INNO-206 and conventional doxorubicin administered weekly in vivo using our severe combined immunodeficient (SCID) murine model of human MM LAGκ-1A. We used the MTS cell proliferation and Annexin V assays for determining the number of viable cells and apoptosis, respectively following exposure to INNO-206 at pH 5, 6 and 7. Our CAM/FB model was used to assess the anti-angiogenic effects of this drug at these same pH levels. For the in vivo studies, each SCID mouse received a 20 – 40 mm3 MM tumor piece surgically implanted into the left hind limb superficial gluteal muscle. Seven days post-implantation mice were bled, human IgG levels were measured by ELISA and mice randomized into treatment groups. INNO-206 was diluted in sodium phosphate and administered to SCID mice at 10.8 mg/kg (free doxorubicin equivalent of 7 mg/kg) once weekly via intravenous injection (i.v.). Conventional doxorubicin (Sigma, St Louis, MO) was also diluted in sodium phosphate and mice were treated with the drug at 4 or 8 mg/kg i.v. once weekly. Mice were bled for hIgG levels and the intramuscular tumors were measured using standard calipers on a weekly basis. Data was analyzed as the mean ± SEM. INNO-206 increased MM cell apoptosis and reduced cell proliferation much more markedly at pH 5 compared to pH 7. Using our CAM/FB model to evaluate angiogenesis, INNO-206 inhibited blood vessel and feather formation as well as endothelial gene and protein expression within FB in a concentration- and pH-dependent fashion. Moreover, SCID mice bearing LAGκ-1A treated with INNO-206 at 10.8 mg/kg showed markedly smaller tumor volumes and IgG levels on days 28 (tumor volumes: P=0.0152; hIgG: P=0.0019), 35 (tumor volumes: P=0.0051; hIgG: P=0.0006) and 42 (tumor volumes: P=0.0036; hIgG: P=0.0113) compared to vehicle-treated mice. Overall, 90% of mice were alive for the duration of the study (day 42). In contrast, treatment of tumor-bearing mice with doxorubicin at 4 and 8 mg/kg resulted in significant toxicity and death (4 mg/kg resulted in 3/10 and 9/10 deaths on days 28 and 35, respectively; 8 mg/kg, 5/10, 8/10 and 10/10 deaths occurred on days 21 28 and 35, respectively). We have shown that the novel anthracycline INNO-206 shows marked anti-MM effects both in vitro and in vivo using our SCID-hu MM model LAGκ-1A. Moreover, this albumin-bound drug which is released only under acidic conditions which is commonly present within tumor tissue is able to be administered safely at higher doses than conventional doxorubicin. The drug also shows marked anti-angiogenic effects in a pH-dependent fashion. The results from these studies suggest that this doxorubicin conjugate may provide MM patients with a new anthracycline that may be able to be administered at higher doses safely resulting in superior efficacy compared to the currently available anthracyclines to treat this B-cell malignancy. Disclosures: Berenson: CytRx Corporation: Research Funding.

QUALIDADE DA BERINJELA IRRIGADA COM ÁGUAS SALOBRAS VIA GOTEJAMENTO CONTÍNUO E POR PULSOS LUCAS FARIAS DAMASCENO1; LOUISE ROSA MONTE BELO2; HANS RAJ GHEYI3; ALIDE MITSUE WATANABE COVA4; GEOVANI SOARES DE LIMA5 E LARA DE JESUS MARQUES6 1 Mestrando em Engenharia Agrícola da Universidade Federal do Recôncavo da Bahia, Rui Barbosa, 710, Centro, 45330-000, Cruz das Almas, Bahia, Brasil. E-mail: lucas_farias13@hotmail.com 2 Engenheira Agrônoma, Universidade Federal do Recôncavo da Bahia, Rui Barbosa, 710, Centro, 45330-000, Cruz das Almas, Bahia, Brasil. E-mail: louiserosamonte@gmail.com 3 Professor Visitante da Universidade Federal do Recôncavo da Bahia, Rui Barbosa, 710, Centro, 45330-000, Cruz das Almas, Bahia, Brasil. E-mail: hgheyi@gmail.com. 4 Pós-Doutoranda no Programa de Pós-graduação de Engenharia Agrícola da Universidade Federal do Recôncavo da Bahia, Rui Barbosa, 710, Centro, 45330-000, Cruz das Almas, Bahia, Brasil. E-mail: alidewatanabe@yahoo.com.br 5 Programa de Pós-Graduação em Engenharia Agrícola, Universidade Federal de Campina Grande, Aprígio Veloso, 882, Universitário, 58428-830, Campina Grande, Paraíba, Brasil. E-mail:geovani.soares@pq.cnpq.br 6 Estudante de Agronomia da Universidade Federal do Recôncavo da Bahia, Rui Barbosa, 710, Centro, 45330-000, Cruz das Almas, Bahia, Brasil. E-mail: lara_marques8@hotmail.com 1 RESUMO Devido suas propriedades medicinais e por ser um alimento rico em antioxidantes, o consumo da hortaliça berinjela vem crescendo. Durante o cultivo da berinjela, o manejo da água e do solo são fatores que podem influenciar a qualidade do fruto. Assim, objetivou-se com este trabalho analisar as características físico-químicas do fruto da berinjela ‘Florida Market’ irrigada com águas salobras por gotejamento contínuo e pulsos. O experimento foi conduzido em ambiente protegido com delineamento experimental em blocos casualizados, em esquema fatorial 2 x 4, com cinco repetições. Os tratamentos consistiram de duas formas de aplicação de água: gotejamento contínuo e pulsos, com quatro níveis de condutividade elétrica da água – CEa (0,3 (controle); 1,5; 3,0 e 4,5 dS m-1). Foram avaliadas as variáveis: massa fresca do fruto, teor de sólidos solúveis totais, pH da polpa, acidez total titulável e relação sólidos solúveis totais/acidez total titulável. Com o incremento da salinidade da água de irrigação o teor de sólidos solúveis totais (+3,60%) e acidez total titulável (+9,87%) aumentaram, enquanto a relação de sólidos solúveis totais/acidez total titulável (-4,53%) e a massa fresca do fruto diminuíram. A interação entre a salinidade e a forma de aplicação da água não influenciou as características físico-químicas do fruto. Palavras-chave: Solanum melongena L., salinidade, sólidos solúveis, acidez titulável. DAMASCENO, L. F.; BELO, L. R. M.; GHEYI, H. R.; COVA, A. M. W.; LIMA, G. S. de; MARQUES, L. de J. QUALITY OF EGGPLANT FRUIT IRRIGATED WITH BRACKISH WATER UNDER CONTINUOUS DRIP AND PULSE IRRIGATION 2 ABSTRACT Due to its medicinal properties and to be a functional food rich in antioxidants, the eggplant vegetable consumption has been increasing. During eggplant cultivation, water and soil management are factors that can influence fruit quality. Therefore, this work aimed to analyze the physico-chemical characteristics of the fruit of the eggplant ‘Florida Market’ irrigated with brackish water under continuous drip and pulse irrigation. The experiment was conducted in protected environment in a randomized block design, adopting a 2 x 4 factorial scheme, with five replicates. The treatments consisted of a combination of two forms of application of brackish water: continuous drip and pulses, with four levels of water salinity - ECw (0.3 (control); 1.5; 3.0 and 4.5 dS m-1). The variables evaluated were fresh weight of the fruit, total soluble solids content, pH of the pulp, total titratable acidity and the ratio of total soluble solids/total titratable acidity. With the increase in salinity of irrigation water the content of total soluble solids (+3.60%) and total titratable acidity (+9.87%) increased, while the ratio of total soluble solids/total titratable acidity (-4.53%) and fresh fruit mass decreased. The interaction between salinity and the form of water application did not influence the physico-chemical characteristics of the fruit. Keywords: Solanum melongena L., salinity, soluble solids, titratable acidity.

- Peter Boomgaard, Nancy Lee Peluso, Rich Forests, Poor people; Resource control and resistance in Java. Berkeley, etc.: University of California Press, 1992, 321 pp. - N. A. Bootsma, H.W. Brands, Bound to empire; The United States and the Philippines. New York, Oxford: Oxford University Press, 1992, 356 pp. - Martin van Bruinessen, Jan Schmidt, Through the Legation Window, 1876-1926; Four essays on Dutch, Dutch-Indian and Ottoman history. Istanbul: Nederlands Historisch-Archaeologisch Instituut, 1992, 250 pp. - Freek Colombijn, Manuelle Franck, Quand la rizière recontre l ásphalte; Semis urbain et processus d úrbanisation à Java-est. Paris: École des hautes études en sciences sociales (Études insulindiennes: Archipel 10), 1993, 282 pp. Maps, tables, graphs, bibliography. - Kees Groeneboer, G.M.J.M. Koolen, Een seer bequaem middel; Onderwijs en Kerk onder de 17e eeuwse VOC. Kampen: Kok, 1993, xiii + 287 pp. - R. Hagesteijn, Janice Stargardt, The Ancient Pyu of Burma; Volume I: Early Pyu cities in a man-made landscape. Cambridge: PACSEA, Singapore: ISEAS, 1991. - Barbara Harrisson, Rolf B. Roth, Die ‘Heiligen Töpfe der Ngadju-Dayak (Zentral-Kalimantan, Indonesien); Eine Untersuchung über die rezeption von importkeramik bei einer altindonesischen Ethnie. Bonn (Mundus reihe ethnologie band 51), 1992, xv + 492 pp. - Ernst Heins, Raymond Firth, Tikopia songs; Poetic and musical art of a Polynesian people of the Solomon Islands. Cambridge: Cambridge University Press (Cambridge studies in oral and literate culture no. 20), 1990, 307 pp., Mervyn McLean (eds.) - Ernst Heins, R. Anderson Sutton, Traditions of gamelan music in Java; Musical pluralism and regional identity.Cambridge: Cambridge University Press (Cambridge studies in ethnomusicology), 1991, 291 pp., glossary, biblio- and discography, photographs, tables, music. - H.A.J. Klooster, Jaap Vogel, De opkomst van het indocentrische geschiedbeeld; Leven en werken van B.J.O. Schrieke en J.C. van Leur. Hilversum: Verloren, 1992, 288 pp. - Jane A. Kusin, Brigit Obrist van Eeuwijk, Small but strong; Cultural context of (mal)nutrition among the Northern Kwanga (East Sepik province, Papua New Guinea). Basel: Wepf & Co. AG Verlag, Basler Beiträge zur ethnologie, Band 34, 1992, 283 pp. - J. Thomas Lindblad, Pasuk Phongpaichit, The new wave of Japanese investment in ASEAN. Singapore: Institute of Southeast Asian studies, 1990, 127 pp. - Niels Mulder, Louis Gabaude, Une herméneutique bouddhique contemporaine de Thaïlande; Buddhadasa Bhikku. Paris: École Francaise d’Extrême-Orient, 1988, vii + 692 pp. - Marleen Nolten, Vinson H. Sutlive. Jr., Female and male in Borneo; Contributions and challenges to gender studies. Borneo research council Monograph series, volume 1, not dated but probably published in 1991. - Ton Otto, G.W. Trompf, Melanesian Religion. Cambridge: Cambridge University Press, 1991, xi + 283 pp., including select bibliography and index. - IBM Dharma Palguna, Gordon D. Jensen, The Balinese people; A reinvestigation of character. Singapore-New York: Oxford University Press, 1992, 232 pp., Luh Ketut Suryani (eds.) - Anton Ploeg, Jürg Schmid, Söhne des Krokodils; Männerhausrituale und initiation in Yensan, Zentral-Iatmul, East Sepik province, Papua New Guinea. Basel: ethnologisches seminar der Universitat und Musuem für Völkerkunde (Basler Beiträge zur ethnologie, band 36), 1992, xii + 321 pp., Christine Kocher Schmid (eds.) - Raechelle Rubinstein, W. van der Molen, Javaans Schrift. (Semaian 8). Leiden: Vakgroep talen en culturen van Zuidoost-Azië en Oceanië, Rijksuniversiteit te Leiden, 1993. x + 129 pp. - Tine G. Ruiter, Arthur van Schaik, Colonial control and peasant resources in Java; Agricultural involution reconsidered. Amsterdam: Koninklijk Nederlands Aardrijkskundig Genootschap/Instituut voor Sociale geografie Universiteit van Amsterdam, 1986, 210 pp. - R. Schefold, Andrew Beatty, Society and exchange in Nias. Oxford: Clarendon press, (Oxford studies in social and cultural Anthropology), 1992, xiv + 322 pp., ill. - N.G. Schulte Nordholt, Ingo Wandelt, Der Weg zum Pancasila-Menschen (Die pancasila-Lehre unter dem P4-Beschlusz des Jahres 1978; Entwicklung und struktur der indonesischen staatslehre). Frankfurt am Main-Bern-New York-Paris: Peter Lang, Europäische Hochschulschriften, Reihe XXVII, Asiatische und Afrikaner Studien, 1989, 316 pp. - J.N.B. Tairas, Herman C. Kemp, Annotated bibliography of bibliographies on Indonesia. Leiden: KITLV press (Koninklijk Instituut voor taal-, land-en Volkenkunde, biographical series 17), 1990, xvii + 433 pp. - Brian Z. Tamanaha, Christopher Weeramantry, Nauru; Environmental damage under international trusteeship. Melbourne (etc.): Oxford University Press, 1992, xx+ 448 pp. - Wim F. Wertheim, Hersri Setiawan, Benedict R.O.’G. Anderson, Language and power; Exploring political cultures in Indonesia. Ithaca/London: Cornell University Press, 1930, 305 pp.

Abstract Background Antibiotics elicit intestinal dysbiosis with short and long-term deleterious effects. A colon-targeted adsorbent, DAV132, prevents dysbiosis in healthy humans and may protect antibiotic-treated patients. Methods Hospitalized patients receiving oral/iv fluoroquinolones (FQ) for the treatment of or prophylaxis of febrile neutropenia were randomized to receive DAV132 (7.5g tid orally), or not, during FQ receipt and followed up 51d. Plasma FQ levels were assessed at D4 (LC-MS/MS). Feces were collected during and up to 30d after FQ receipt for assessment of free fecal FQ levels (LC-MS/MS), gut microbiome α/β diversity (16S rRNA), resistance to colonization by C. difficile (Cd; ex-vivo proliferation). Relatedness of adverse events (AEs) to drugs was adjudicated by blinded independent experts. Results 243 patients from 23 sites, median age 71y, ≥1 chronic comorbidity 95%, received levofloxacin (43%), ciprofloxacin (40%) or moxifloxacin (18%) for (79% iv). During receipt, fecal FQ levels were lowered by &gt;97% with DAV132 vs. No DAV132 (p&lt; 0.0001), whilst plasma levels did not change significantly. Microbiome diversity was significantly protected with DAV132 using all metrics, e.g. the change from D1 of Shannon index at End-of-FQ (difference of means at End-of-FQ 0.42, 95% CI: 0.085; 0.752). The proportions of patients with DAV132- and/or FQ-related AEs (primary endpoint) did not differ significantly (14.8 vs. 10.8%, difference of proportions: 3.9%; 95% CI: -4.7; 12.6). No Cd infection occurred. Resistance to colonization by Cd was reduced in stools of patients receiving FQ only, but was maintained in those of patients who also received DAV132 (p=0.035). The acquisition of fecal carriage of vancomycin-resistant enterococci (VRE) was reduced with DAV132 (p=0.019). Figure 1: a. Free fluoroquinolones fecal concentration (mean ± SEM, µg/g) over time per FQ treatment group; b. Change of Shannon Index from baseline (mean ± SEM) over time Conclusion DAV132 was well tolerated in elderly hospitalized patients with comorbidities. It neither altered antibiotic plasma levels nor elicited changes in concomitant drugs regimens. Intestinal microbiota diversity was protected and resistance to colonization by Cd was preserved. DAV132 is a promising, novel product to prevent antibiotic-induced intestinal dysbiosis. Disclosures Annie Ducher, MD, Da Volterra (Employee, Shareholder) Maria J.G.T. Vehreschild, n/a, 3M (Grant/Research Support)Astellas Pharma (Grant/Research Support)Astellas Pharma (Consultant)Astellas Pharma (Speaker's Bureau)Basilea (Speaker's Bureau)Berlin Chemie (Consultant)Da Volterra (Grant/Research Support)Da Volterra (Grant/Research Support)Gilead (Grant/Research Support)Gilead (Speaker's Bureau)Merck/MSD (Speaker's Bureau)Merck/MSD (Grant/Research Support)MSD/Merck (Consultant)Organobalance (Grant/Research Support)Organobalance (Speaker's Bureau)Pfizer (Speaker's Bureau)Seres Therapeutics (Grant/Research Support) Thomas J. Louie, MD, Da Volterra (Consultant) Oliver A. Cornely, MD, Actelion (Consultant, Grant/Research Support, Speaker's Bureau)Al Jazeera Pharmaceuticals (Consultant)Allecra Therapeutics (Consultant, Grant/Research Support, Speaker's Bureau)Amplyx (Consultant, Grant/Research Support, Speaker's Bureau)Astellas (Consultant, Grant/Research Support, Speaker's Bureau)Basilea (Consultant, Grant/Research Support, Speaker's Bureau)Biosys UK Limited (Consultant, Grant/Research Support, Speaker's Bureau)Cidara (Consultant, Grant/Research Support, Speaker's Bureau)Da Volterra (Consultant, Grant/Research Support, Speaker's Bureau)Entasis (Consultant, Grant/Research Support, Speaker's Bureau)European Commission (Grant/Research Support)F2G (Consultant, Grant/Research Support, Speaker's Bureau)German Federal Ministry of Research andEducation (Grant/Research Support)Gilead (Consultant, Grant/Research Support, Speaker's Bureau)Grupo Biotoscana (Consultant, Grant/Research Support, Speaker's Bureau)Janssen Pharmaceuticals (Consultant, Grant/Research Support, Speaker's Bureau)Matinas (Consultant, Grant/Research Support, Speaker's Bureau)MedicinesCompany (Consultant, Grant/Research Support, Speaker's Bureau)MedPace (Consultant, Grant/Research Support, Speaker's Bureau)Melinta Therapeutics (Consultant, Grant/Research Support, Speaker's Bureau)Menarini Ricerche (Consultant, Grant/Research Support, Speaker's Bureau)Merck/MSD (Consultant, Grant/Research Support, Speaker's Bureau)Mylan Pharmaceuticals (Consultant)Nabriva (Consultant)Noxxon (Consultant)Octapharma (Consultant, Grant/Research Support, Speaker's Bureau)Paratek Pharmaceuticals (Consultant, Grant/Research Support, Speaker's Bureau)Pfizer (Consultant, Grant/Research Support, Speaker's Bureau)PSI (Consultant, Grant/Research Support, Speaker's Bureau)Roche Diagnostics (Consultant)Scynexis (Consultant, Grant/Research Support, Speaker's Bureau)Shionogi (Consultant) Céline Féger, PhD, Da Volterra (Consultant) Aaron Dane, MSc, Da Volterra (Consultant)Spero theraputics (Consultant) Aaron Dane, MSc, Spero theraputics (Consultant) Marina Varastet, PhD, Da Volterra (Employee) Jean de Gunzburg, PhD, Da Volterra (Board Member, Consultant, Shareholder) Antoine Andremont, PhD, Bioaster (Consultant)Da Volterra (Board Member, Consultant, Shareholder) France Mentré, MD, Da Volterra (Consultant)

Introduction: Nasopharyngeal carcinoma (NPC) is defined as the cancer of squamous epithelium lining nasopharynx. The single most common culprit of undifferentiated NPC is the Epstein-Barr virus (EBV). Recurrent local-regional or metastatic NPC cannot be treated with repeated chemo-radiotherapy because of poor overall survival and profound effect of these therapies on quality of life. One safer approach is immunotherapy with autologous EBV specific cytotoxic T lymphocytes (EBV-CTLS) targeted to the EBV antigens EBNA1, latent membrane protein LMP1, and LMP2 expressed by most NPC tumors. This study aims to review the efficacy and toxicity of adoptive immunotherapy with EBV-CTLS in patients with EBV induced NPC. Methods: A systematic search of PubMed, Embase, Clinicaltrials.gov, and Web of Science was performed for adoptive immunotherapy in EBV induced NPC patients from inception to May 28, 2020. Out of 604 studies, 07 phase I and II clinical trials were selected for the systematic review. Results: A total of 134 patients (pts) were evaluated out of 157 pts. 56 had a locoregional disease, 63 had distant metastasis, 15 had both locoregional disease as well as distant metastasis, 8 were in remission and disease status was unknown in 5 pts. Li et al. (2015) in their phase I clinical trial on 20 NPC pts with ECOG performance status of &lt;3 after chemoradiotherapy (CCRT) showed overall response rate (ORR) of 95% with complete response (CR) in 19 patients. One patient showed progressive disease (PD). Median progression-free survival (PFS) was observed to be 16 months. Eighteen (90%) pts showed disease-free survival of greater than 12 months after adoptive cell therapy (ACT). Grade (G) ≥3 adverse events (AEs) included leukopenia (5%) and neutropenia (5%). Phase I/II dose-escalation trial by Louis et al. (2010) on 23 pts showed ORR of 48.7% (20% CR, 13.3% undetermined complete response [Cru], 15.4% PR) among pts with active disease. Eight pts remained in remission while 10 had metastatic disease at the time of infusion. PD was 21.7%, Stable Disease (SD) 13%, and 3 pts (13%) had recurrent disease. The median time to progression was 1059 days with PFS of 65% and 52% at 1 and 2 years respectively while the (Overall Survival) OS was 87% and 70% at 1 year and 2 years respectively. There was a higher risk of disease progression (HR: 3.91, P= 0.015) and decreased overall survival (HR: 5.55, P=0.022) in metastatic disease as compared to locoregional disease. Huang J. et al (2017) conducted a phase I/ II trial in 21 pts with a mean waiting period of 71 days after chemotherapy. Two CTL infusions were given 2 weeks apart. Two pts (9.5%) maintained SD but all other pts (85%) showed PD after 8 weeks follow- up. One patient achieved CR (4.8%). Hence, ORR was 4.8% while median PFS and OS were of 2.2 months and 16.7 months respectively. In a phase II trial, 24 patients completed 6 EBV- CTL therapy cycles after receiving chemotherapy cycles of Gemcitabine and Carboplatin. ORR was observed to be 42.9% (CR 5.7%, PR 31.7%). SD was 20% while PD was 31.4%. Median OS was 29.9 months (95% CI 20.8-39.3) with 1, 2, and 3-year rates being 77.1%, 62.9%, and 37.1 % respectively. Median PFS was 7.6 months (95% CI 7.4-8.4). All G≥3 AE occurred during chemotherapy. (Chia et al, 2014) Secondino et al. (2011) conducted a phase I/II study in 11 NPC patients who also received chemotherapy consisting of cyclophosphamide and fludarabine. After a mean follow-up of 4 weeks, ORR was 27% (PR 18%, Minor Response [MR] 9%). PD was reported to be 45% and SD 27%. Median PFS at 6 months was 54% (6/11 pts). Only G≥3 AE reported was neutropenia (36%). Phase I/II trial by Comoli et al. (2005) evaluated 10 EBV-related stage IV NPC in progression after CCRT. After receiving two to twenty-three EBV-specific CTLs infusions, 2 patients showed PR (20%), 40% of pts maintained SD and all others showed evidence of PD (40%) at 1-2 months follow up. Median PFS was 6.5 months. Smith et al (2012) in their phase I trial on 14 patients with locoregional and metastatic NPC reported SD 71.4% and PD 28.6% of patients at a median follow up of 1 month. Median OS and PFS were 17.4 months and 4.5 months respectively. No G≥3 AEs were reported. Conclusion : Adoptive Immunotherapy with EBV-CTLS has shown impressive efficacy with improvement in median PFS and OS and a favorable safety profile. Key Words: Adoptive cell therapy, Cytotoxic T lymphocytes, nasopharyngeal carcinoma, Phase I/II clinical trials, Epstein-Barr virus. Disclosures Anwer: Celgene: Research Funding; AbbVie Pharmaceuticals: Research Funding; Incyte Pharmaceuticals: Consultancy, Honoraria, Other: Travel, Accommodations, Expenses, Speakers Bureau; Astellas Pharma: Research Funding; Acetylon Pharmaceuticals: Research Funding; Seattle Genetics: Consultancy, Honoraria, Other: Travel, Accommodations, Expenses, Speakers Bureau; Millennium Pharmaceuticals: Research Funding.

Abstract Abstract 4275 Introduction: There is interest improving induction chemotherapy in acute myeloid leukemia. The PALG (Polish Adult Leukemia Group) demonstrated that the addition of cladribine to daunorubicin/cytarabine resulted in improved CR rates and overall survival in untreated AML patients <60 years of age (Blood{ASH Annual Meeting Abstracts}, Nov 2009; 114: 2055). Idarubicin has shown superiority over high dose daunorubicin in the Acute Leukemia French Association 9801 Study (J Clin Oncol 2010, 28: 808–814), and here we present results of idarubicin/cytarabine/cladribine (IAC) therapy in untreated AML. Methods: Records of all patients receiving cladribine at Saint Louis University Hospital between December 2008 and July 2011 were analyzed after identification using a pharmacy database search. We began using cladribine for all acute myeloid leukemia patients after the report of improved overall survival in December 2008 (Blood{ASH Annual Meeting Abstracts}, Nov 2008; 112: 133). All patients with untreated acute myeloid leukemia by World Health Organization (WHO) criteria were included who received induction with IAC, which consisted of: idarubicin 12mg/m2 intravenously (IV) daily day 1–3, cytarabine 200mg/m2 IV daily day 1–7 by continuous infusion, and cladribine 5mg/m2 IV daily day 1–5. Results: 34 patients received the IAC regimen. International Working Group criteria were used to assess response and cytogenetic abnormalities were grouped according to SWOG criteria. Median age was 60 years (range 25–81). Age: < 60: 18/34 (53 %), ≥ 60: 16/34 (47 %). 15/34 (44 %) had ECOG performance status 1–2, and 19/34 (56 %) had performance status 3–4. Median WBC count on presentation was 11 K (range 1K–483K). Risk distribution by cytogenetics: favorable: 4/34 (12 %), intermediate: 20/34 (59 %), adverse: 10/34 (29%) and by molecular analysis with NPM1/FLT3-ITD/CEBPA: favorable: 7/34 (21 %), intermediate-1: 12/34 (35 %), intermediate-2: 5/34 (15 %), adverse: 10/34 (29 %). The ORR (CR+CRi): 22/34 (65 %). CR 21, Cri 1. ORR by age: < 60: 14/18 (78 %), ≥ 60: 8/16 (50 %). ORR by cytogenetics: favorable: 4/4 (100%), intermediate: 14/20 (70 %), adverse: 4/10 (40 %). CR/CRi in 1 course: 18/20 (90%). Treatment failure (TF): 12 (37%). Treatment failure was due to persistent leukemia in 6/12 (50 %), death in bone marrow aplasia in 5/12 (42 %), and death due to indeterminate cause in 2/12 (8 %). Further consolidation treatment was heterogenous and typically consisted of either HIDAC or allogeneic transplantation depending on risk features. The median observation time of this patient cohort was 16.7 months (range: 4.1–29.4 months). The median event-free survival and overall survival were 9.5 months (range: 1.4–17.6 months) and 16.7 months (range: 5.1–28.3), respectively. See Figures1 and2. The 30 and 60 day mortality rates were 3/34 (9 %) and 7/34 (21 %), respectively. The median time to hospital discharge in those achieving remission was 26.5 days (range: 21–80 days). The median day to achievement of a neutrophil count >500 was 23 (range 21–43) in those who achieved a CR in 1 course. The median day to achievement of platelet transfusion independence with transfusion threshold of 10K and to platelet count ≥ 50K was 21 (range 19–28) and 23 (range 20–26), respectively, in those who achieved a CR in 1 course. The median number of red blood cell and platelet transfusions in those who achieved CR in 1 course was 8 (range 2–18) and 6 (range 3–18), respectively. Death in aplasia etiologies were (all in 1st IAC induction unless otherwise noted): autopsy-proven Aspergillus infections, one disseminated day 14, one pulmonary day 16/ MDR Pseudomonas sepsis and toxic megacolon day 21/ respiratory failure of undetermined etiology with recent stroke day 16 of 2nd IAC induction. Indeterminate deaths were: shock of undetermined etiology with multiorgan failure day 16 / probable Zygomycete pulmonary infection day 37. 25/34 patients (74%) had documented infection during induction. Conclusion: We found a high complete remission rate of IAC in younger patients with an acceptable 30 day mortality rate in spite of short follow-up. We believe that the addition of cladribine to induction chemotherapy with intensified daunorubicin or idarubicin for acute myeloid leukemia warrants further study. Disclosures: Off Label Use: Cladribine was utilized off-label in this study based on prior reports of efficacy in untreated and relapsed acute myeloid leukemia patients.

The aim of the article is to describe psycholinguistic meanings of the word-stimulus “playfulness” in the linguistic world-image of the Russian-speaking population of Ukraine. The main method of the conducted research was the psycholinguistic experiment. The sample according to the criteria “gender” (males and females – included both age groups) and “age” (18-35 and 36-60) included 1,600 respondents with 400 people in each sub-group of respondents. The overall number of reactions to stimulus “playfulness” comprised 1,600 associative reactions with 475 unique associations including word combinations and sentences, where 159 reactions have frequency over 1,316 individual associations, and 0 refusals. The semantic interpretation of the results of the free association test made it possible to single out 19 psycholinguistic meanings, 12 out of them accounted for more than 1%: 1) “cheerful and joyful state”, 2) “intention to attract the attention of the opposite or one’s own sex”, 3) “child-like spontaneity”, 4) “agility, physical activity of an animal”, 5) “daring and provocative behavior”, 6) “agility, physical behavior of a human being”, 7) “ease”, 8) “changeability”, 9) “behavior during a sexual intercourse”, 10) “carelessness”, 11) “mental activity”, 12) “deliberate deceit”. Taking into account the respondents’ verbal behavior, the following components of playfulness were identified: flirting, impishness, humor, fugue (eccentricity), ease, imagination. The formulated psycholinguistic meanings of playfulness can be fully considered as such that give the most adequate and reliable model of the systemic significance of the studied word and which reflects the reality of linguistic consciousness. References Barnett, L. A. (2007). The nature of playfulness in young adults. Personality and Individual Differences, 43, 949-958. Bowman, J. R. (1987). Making work play. In: Meaningful play, playful meanings. (pp. 61-71). G.A. Fine (Ed.), Champaign, IL: Human Kinetics. Bundy, A. C. (1996). Play and playfulness: what to look for. In: Play in Occupational Therapy for Children, (pp. 52-66). D. L. Parham & L. S. Fazio (Eds.). St. Louis, MO: Mosby. Chandler, B. E. (1997). The essence of play: a child’s occupation. Bethesda, MD: American Occupational Therapy Association, Inc. Glynn, M. A., Webster, J. (1992). The adult playfulness scale: An initial assessment. Psycho­logical Reports, 71(1), 83-103. Gordiienko-Mytrofanova, I. V. (2014a). Leksikograficheskoie znacheniie slova “igrivost” (podgo­to­­­vitelnyi etap psikholingvisticheskogo eksperimenta) [The lexicographic meaning of the word “playfulness” (the preparatory stage of the psycholinguistic experiment)]. Psychological Prospects Journal, 24, 65-77. Gordiienko-Mytrofanova, I. V. (2014b). Psikhologicheskoie soderzhaniie leksikograficheskikh znachenii slova “igrivyi” (podgotovitelnyi etap psikholingvisticheskogo eksperimenta) [The psychological content of the lexicographic meanings of the word “playful” (the preparatory stage of the psycholinguistic experiment)]. Problemy suchasnoi pedahohichnoi osvity – Problems of Modern Pedagogical Education, 45(2), 419-430. Gordiienko-Mytrofanova, I. V. (2014c). Psikhologicheskaia interpretatsiia leksikograficheskogo opisaniia slova “igrivyi” [Psychological interpretation of the lexicographic description of the word “playful”]. Problemy Suchasnoi Psykholohii – Problems of Modern Psychology, 25, 83-98. Gordienko-Mytrofanova, I., Sypko, A. (2015). Playfulness as a relevant lexeme in the bilingual linguistic consciousness of Ukrainian people. East European Journal of Psycholinguistics, 2(1), 43-51. Gordienko-Mytrofanova, I., Sauta, S. (2016). Playfulness as a peculiar expression of sexual relationships (semantic interpretation of the results of the psycholinguistic experi­ment). European Humanities Studies: State and Society, 1, 46-62. Gordienko-Mytrofanova, I., Kobzieva, I. (2017). Humor as a component of ludic competence. Visnyk [Journal] of the Hryhorii Skovoroda Kharkiv National Pedagogical University, 57, 40-56. Gordienko-Mytrofanova, I., Kobzieva, I. (2018). Concept «Holy Fool» in the Linguistic World-Image of the Russian-Speaking Population of Ukraine. Psycholinguistics, 24(1), 118-133. Gordiienko-Mytrofanova, I., Pidchasov, Y., Sauta, S. Kobzieva, I. (2018). The problem of sample representativeness for conducting experimental and broad psychological research. Psycholinguistics, 23(1), 11-46. Guitard, P., Ferland, F., & Dutil, É. (2005). Toward a better understanding of playfulness in adults. OTJR: Occupation, Participation and Health, 25(1), 9-22. Kondakov, N. I. (1975). The Logical Dictionary-Reference. Moscow: Nauka. Proyer, R. T. (2012). A Psycho-linguistic Study on Adult Playfulness: Its Hierarchical Structure and Theoretical Considerations. Journal of Adult Development, 19(3), 141-149. Proyer, R.T. (2014). A Psycho-Linguistic Approach For Studying Adult Playfulness: A Replication and Extension Toward Relations With Humor, The Journal of Psychology, 148(6), 717-735. Proyer, R.T. (2017). A new structural model for the study of adult playfulness: Assessment and exploration of an understudied individual differences variable. Personality and Individual Differences, 108, 113-122. Rudakova, A. V. (2015). On the concept of an integrated lexicographic meaning of a word and the methodology of its description. Culture of Communication and Its Formation: Interuniversity Collection of Scientific Works, 31, 109-115. Schaefer, C. & Greenberg, R. (1997). Measurement of Playfulness: A Neglected Therapist Variable. International Journal of Play Therapy, 6(2), 21-31. Shen, X. (2010). Adult playfulness as a personality trait: Its conceptualization, measurement, and relationship to psychological well-being. Doctoral dissertation. Retrieved from Pennsylva­nia State University Library Catalog (OCLC No. 859524715). Sternin, I.A., & Rudakova, A.V. (2011). Psikholingvisticheskoie znacheniie slova i yego opisaniie [Psycholinguistic meaning of the word and its description]. Voronezh: Lambert. Tsuji, Hit., Tsuji, Hei., Yamada, S., Natsuno, Y., Morita, Y., Mukoyama, Y., Hata, K., Fujishima, Y. (1996). Standardization of the Five Factor Personality Questionnaire: Factor structure. International Journal of Psychology, 31. Proceedings from the XXVI International Congress of Psychology. August 16–21, 1996, Montreal, Canada. (103-217). Ufimtseva, N. V. (2009). Obraz mira russkikh: sistemnost i soderzhaniie [Image of the world of Russians: the systemic characteristics and the content]. Yazyk i kultura – Language and Culture, 98-111. Yarnal, C., & Qian, X. (2011). Older-adult Playfulness: An innovative construct and measure­ment for healthy aging research. American Journal of Play, 4(1), 52-79. Yue, X. D., Leung, C. L., Hiranandani, N. A. (2016). Adult Playfulness, Humor Styles, and Subjective Happiness. Psychological Reports, 119(3), 630-640.

Hamsters were exposed to sodium arsenite (173 mg As/L) in drinking water for 6 days. Equal amounts of proteins from urinary bladder or liver extracts of control and arsenic-treated hamsters were labeled with Cy3 and Cy5 dyes, respectively. After differential in gel electrophoresis and analysis by the DeCyder software, several protein spots were found to be down-regulated and several were up regulated. Our experiments indicated that in the bladder tissues of hamsters exposed to arsenite, transgelin was down-regulated and GST-pi was up-regulated. The loss of transgelin expression has been reported to be an important early event in tumor progression and a diagnostic marker for cancer development [29-32]. Down-regulation of transgelin expression may be associated with the carcinogenicity of inorganic arsenic in the urinary bladder. In the liver of arsenite-treated hamsters, ornithine aminotransferase was up-regulated, and senescence marker protein 30 and fatty acid binding protein were down-regulated. The volume ratio changes of these proteins in the bladder and liver of hamsters exposed to arsenite were significantly different than that of control hamsters. Introduction Chronic exposure to inorganic arsenic can cause cancer of the skin, lungs, urinary bladder, kidneys, and liver [1-6]. The molecular mechanisms of the carcinogenicity and toxicity of inorganic arsenic are not well understood [7-9). Humans chronically exposed to inorganic arsenic excrete MMA(V), DMA(V) and the more toxic +3 oxidation state arsenic biotransformants MMA(III) and DMA (III) in their urine [10, 11], which are carcinogen [12]· After injection of mice with sodium arsenate, the highest concentrations of the very toxic MMA(III) and DMA(III) were in the kidneys and urinary bladder tissue, respectively, as shown by experiments of Chowdhury et al [13]. Many mechanisms of arsenic toxicity and carcinogenicity have been suggested [1, 7, 14] including chromosome abnormalities [15], oxidative stress [16, 17], altered growth factors [18], cell proliferation [19], altered DNA repair [20], altered DNA methylation patterns [21], inhibition of several key enzymes [22], gene amplification [23] etc. Some of these mechanisms result in alterations in protein expression. Methods for analyzing multiple proteins have advanced greatly in the last several years. In particularly, mass spectrometry (MS) and tandem MS (MS/MS) are used to analyze peptides following protein isolation using two-dimensional (2-D) gel electrophoresis and proteolytic digestion [24]. In the present study, Differential In Gel Electrophoresis (DIGE) coupled with Mass Spectrometry (MS) has been used to study some of the proteomic changes in the urinary bladder and liver of hamsters exposed to sodium arsenite in their drinking water. Our results indicated that transgelin was down-regulated and GST-pi was up-regulated in the bladder tissues. In the liver tissues ornithine aminotransferase was up-regulated, and senescence marker protein 30, and fatty acid binding protein were down-regulated. Materials and Methods Chemicals Tris, Urea, IPG strips, IPG buffer, CHAPS, Dry Strip Cover Fluid, Bind Silane, lodoacetamide, Cy3 and Cy5 were from GE Healthcare (formally known as Amersham Biosciences, Uppsala, Sweden). Thiourea, glycerol, SDS, DTT, and APS were from Sigma-Aldrich (St. Louis, MO, USA). Glycine was from USB (Cleveland, OH, USA). Acrylamide Bis 40% was from Bio-Rad (Hercules, CA, USA). All other chemicals and biochemicals used were of analytical grade. All solutions were made with Milli-Q water. Animals Male hamsters (Golden Syrian), 4 weeks of age, were purchased from Harlan Sprague Dawley, USA. Upon arrival, hamsters were acclimated in the University of Arizona animal care facility for at least 1 week and maintained in an environmentally controlled animal facility operating on a 12-h dark/12-h light cycle and at 22-24°C. They were provided with Teklad (Indianapolis, IN) 4% Mouse/Rat Diet # 7001 and water, ad libitum, throughout the acclimation and experimentation periods. Sample preparation and labelling Hamsters were exposed to sodium arsenite (173 mg) in drinking water for 6 days and the control hamsters were given tap water. On the 6th day hamsters were decapitated rapidly by guillotine. Urinary bladder tissues and liver were removed, blotted on tissue papers (Kimtech Science, Precision Wipes), and weighed. Hamster urinary bladder or liver tissues were homogenized in lysis buffer (30mMTris, 2M thiourea, 7M urea, and 4% w/w CHAPS adjusted to pH 8.5 with dilute HCI), at 4°C using a glass homogenizer and a Teflon coated steel pestle; transferred to a 5 ml acid-washed polypropylene tube, placed on ice and sonicated 3 times for 15 seconds. The sonicate was centrifuged at 12,000 rpm for 10 minutes at 4°C. Small aliquots of the supernatants were stored at -80°C until use (generally within one week). Protein concentration was determined by the method of Bradford [25] using bovine serum albumin as a standard. Fifty micrograms of lysate protein was labeled with 400 pmol of Cy3 Dye (for control homogenate sample) and Cy5 Dye (for arsenic-treated urinary bladder or liver homogenate sample). The samples containing proteins and dyes were incubated for 30 min on ice in the dark. To stop the labeling reaction, 1uL of 10 mM lysine was added followed by incubation for 10 min on ice in the dark. To each of the appropriate dye-labeled protein samples, an additional 200 ug of urinary bladderor liver unlabeled protein from control hamster sample or arsenic-treated hamster sample was added to the appropriate sample. Differentially labeled samples were combined into a single Microfuge tube (total protein 500 ug); protein was mixed with an equal volume of 2x sample buffer [2M thiourea, 7M urea, pH 3-10 pharmalyte for isoelectric focusing 2% (v/v), DTT 2% (w/v), CHAPS 4% (w/v)]; and was incubated on ice in the dark for 10 min. The combined samples containing 500 ug of total protein were mixed with rehydration buffer [CHAPS 4% (w/v), 8M urea, 13mM DTT, IPG buffer (3-10) 1% (v/v) and trace amount of bromophenol blue]. The 450 ul sample containing rehydration buffer was slowly pipetted into the slot of the ImmobilinedryStripReswelling Tray and any large bubbles were removed. The IPG strip (linear pH 3-10, 24 cm) was placed (gel side down) into the slot, covered with drystrip cover fluid (Fig. 1), and the lid of the Reswelling Tray was closed. The ImmobillineDryStrip was allowed to rehydrate at room temperature for 24 hours. First dimension Isoelectric focusing (IEF) The labeled sample was loaded using the cup loading method on universal strip holder. IEF was then carried out on EttanIPGphor II using multistep protocol (6 hr @ 500 V, 6 hr @ 1000 V, 8 hr @ 8000 V). The focused IPG strip was equilibrated in two steps (reduction and alkylation) by equilibrating the strip for 10 min first in 10 ml of 50mM Tris (pH 8.8), 6M urea, 30% (v/v) glycerol, 2% (w/v) SDS, and 0.5% (w/v) DTT, followed by another 10 min in 10 ml of 50mM Tris (pH 8.8), 6M urea, 30% (v/v) glycerol, 2% (w/v) SDS, and 4.5% (w/v) iodoacetamide to prepare it for the second dimension electrophoresis. Second dimension SDS-PAGE The equilibrated IPG strip was used for protein separation by 2D-gel electrophoresis (DIGE). The strip was sealed at the top of the acrylamide gel for the second dimension (vertical) (12.5% polyacrylamide gel, 20x25 cm x 1.5 mm) with 0.5% (w/v) agarose in SDS running buffer [25 mMTris, 192 mM Glycine, and 0.1% (w/v) SDS]. Electrophoresis was performed in an Ettan DALT six electrophoresis unit (Amersham Biosciences) at 1.5 watts per gel, until the tracking dye reached the anodic end of the gel. Image analysis and post-staining The gel then was imaged directly between glass plates on the Typhoon 9410 variable mode imager (Sunnyvale, CA, USA) using optimal excitation/emission wavelength for each DIGE fluor: Cy3 (532/580 nm) and Cy5 (633/670 nm). The DIGE images were previewed and checked with Image Quant software (GE Healthcare) where all the two separate gel images could be viewed as a single gel image. DeCyde v.5.02 was used to analyze the DIGE images as described in the Ettan DIGE User Manual (GE Healthcare). The appropriate up-/down regulated spots were filtered based on an average volume ratio of ± over 1.2 fold. After image acquisition, the gel was fixed overnight in a solution containing 40% ethanol and 10% acetic acid. The fixed gel was stained with SyproRuby (BioRad) according to the manufacturer protocol (Bio-Rad Labs., 2000 Alfred Nobel Drive, Hercules, CA 94547). Identification of proteins by MS Protein spot picking and digestion Sypro Ruby stained gels were imaged using an Investigator ProPic and HT Analyzer software, both from Genomic Solutions (Ann Arbor, MI). Protein spots of interest that matched those imaged using the DIGE Cy3/Cy5 labels were picked robotically, digested using trypsin as described previously [24] and saved for mass spectrometry identification. Liquid chromatography (LC)- MS/MS analysis LC-MS/MS analyses were carried out using a 3D quadrupole ion trap massspectrometer (ThermoFinnigan LCQ DECA XP PLUS; ThermoFinnigan, San Jose, CA) equipped with a Michrom Paradigm MS4 HPLC (MichromBiosources, Auburn, CA) and a nanospray source, or with a linear quadrupole ion trap mass spectrometer (ThermoFinnigan LTQ), also equipped with a Michrom MS4 HPLC and a nanospray source. Peptides were eluted from a 15 cm pulled tip capillary column (100 um I.D. x 360 um O.D.; 3-5 um tip opening) packed with 7 cm Vydac C18 (Vydac, Hesperia, CA) material (5 µm, 300 Å pore size), using a gradient of 0-65% solvent B (98% methanol/2% water/0.5% formic acid/0.01% triflouroacetic acid) over a 60 min period at a flow rate of 350 nL/min. The ESI positive mode spray voltage was set at 1.6 kV, and the capillary temperature was set at 200°C. Dependent data scanning was performed by the Xcalibur v 1.3 software on the LCQ DECA XP+ or v 1.4 on the LTQ [27], with a default charge of 2, an isolation width of 1.5 amu, an activation amplitude of 35%, activation time of 50 msec, and a minimal signal of 10,000 ion counts (100 ion counts on the LTQ). Global dependent data settings were as follows: reject mass width of 1.5 amu, dynamic exclusion enabled, exclusion mass width of 1.5 amu, repeat count of 1, repeat duration of a min, and exclusion duration of 5 min. Scan event series were included one full scan with mass range of 350-2000 Da, followed by 3 dependent MS/MS scans of the most intense ion. Database searching Tandem MS spectra of peptides were analyzed with Turbo SEQUEST, version 3.1 (ThermoFinnigan), a program that allows the correlation of experimental tandem MS data with theoretical spectra generated from known protein sequences. All spectra were searched against the latest version of the non redundant protein database from the National Center for Biotechnology Information (NCBI 2006; at that time, the database contained 3,783,042 entries). Statistical analysis The means and standard error were calculated. The Student's t-test was used to analyze the significance of the difference between the control and arsenite exposed hamsters. P values less than 0.05 were considered significant. The reproducibility was confirmed in separate experiments. Results Analysis of proteins expression After DIGE (Fig. 1), the gel was scanned by a Typhoon Scanner and the relative amount of protein from sample 1 (treated hamster) as compared to sample 2 (control hamster) was determined (Figs. 2, 3). A green spot indicates that the amount of protein from sodium arsenite-treated hamster sample was less than that of the control sample. A red spot indicates that the amount of protein from the sodium arsenite-treated hamster sample was greater than that of the control sample. A yellow spot indicates sodium arsenite-treated hamster and control hamster each had the same amount of that protein. Several protein spots were up-regulated (red) or down-regulated (green) in the urinary bladder samples of hamsters exposed to sodium arsenite (173 mg As/L) for 6 days as compared with the urinary bladder of controls (Fig. 2). In the case of liver, several protein spots were also over-expressed (red) or under-expressed (green) for hamsters exposed to sodium arsenite (173 mg As/L) in drinking water for 6 days (Fig. 3). The urinary bladder samples were collected from the first and second experiments in which hamsters were exposed to sodium arsenite (173 mg As/L) in drinking water for 6 days and the controls were given tap water. The urinary bladder samples from the 1st and 2nd experiments were run 5 times in DIGE gels on different days. The protein expression is shown in Figure 2 and Table 1. The liver samples from the 1st and 2nd experiments were also run 3 times in DIGE gels on different days. The proteins expression were shown in Figure 3 and Table 2. The volume ratio changed of the protein spots in the urinary bladder and liver of hamsters exposed to arsenite were significantly differences than that of the control hamsters (Table 1 and 2). Protein spots identified by LC-MS/MS Bladder The spots of interest were removed from the gel, digested, and their identities were determined by LC-MS/MS (Fig. 2 and Table 1). The spots 1, 2, & 3 from the gel were analyzed and were repeated for the confirmation of the results (experiments; 173 mg As/L). The proteins for the spots 1, 2, and 3 were identified as transgelin, transgelin, and glutathione S-transferase Pi, respectively (Fig. 2). Liver We also identified some of the proteins in the liver samples of hamsters exposed to sodium arsenite (173 mg As/L) in drinking water for 6 days (Fig. 3). The spots 4, 5, & 6 from the gels were analyzed and were repeated for the confirmation of the results. The proteins for the spots 4, 5, and 6 were identified as ornithine aminotransferase, senescence marker protein 30, and fatty acid binding protein, respectively (Fig. 3) Discussion The identification and functional assignment of proteins is helpful for understanding the molecular events involved in disease. Weexposed hamsters to sodium arsenite in drinking water. Controls were given tap water. DIGE coupled with LC-MS/MS was then used to study the proteomic change in arsenite-exposed hamsters. After electrophoresis DeCyder software indicated that several protein spots were down-regulated (green) and several were up-regulated (red). Our overall results as to changes and functions of the proteins we have studied are summarized in Table 3. Bladder In the case of the urinary bladder tissue of hamsters exposed to sodium arsenite (173 mg As/L) in drinking water for 6 days, transgelin was down-regulated and GST-pi was up-regulated. This is the first evidence that transgelin is down-regulated in the bladders of animals exposed to sodium arsenite. Transgelin, which is identical to SM22 or WS3-10, is an actin cross linking/gelling protein found in fibroblasts and smooth muscle [28, 29]. It has been suggested that the loss of transgelin expression may be an important early event in tumor progression and a diagnostic marker for cancer development [30-33]. It may function as a tumor suppressor via inhibition of ARA54 (co-regulator of androgen receptor)-enhanced AR (androgen receptor) function. Loss of transgelin and its suppressor function in prostate cancer might contribute to the progression of prostate cancer [30]. Down-regulation of transgelin occurs in the urinary bladders of rats having bladder outlet obstruction [32]. Ras-dependent and Ras-independent mechanisms can cause the down regulation of transgelin in human breast and colon carcinoma cell lines and patient-derived tumorsamples [33]. Transgelin plays a role in contractility, possibly by affecting the actin content of filaments [34]. In our experiments loss of transgelin expression may be associated or preliminary to bladder cancer due to arsenic exposure. Arsenite is a carcinogen [1]. In our experiments, LC-MS/MS analysis showed that two spots (1 and 2) represent transgelin (Fig. 2 and Table 1). In human colonic neoplasms there is a loss of transgelin expression and the appearance of transgelin isoforms (31). GST-pi protein was up-regulated in the bladders of the hamsters exposed to sodium arsenite. GSTs are a large family of multifunctional enzymes involved in the phase II detoxification of foreign compounds [35]. The most abundant GSTS are the classes alpha, mu, and pi classes [36]. They participate in protection against oxidative stress [37]. GST-omega has arsenic reductase activity [38]. Over-expression of GST-pi has been found in colon cancer tissues [39]. Strong expression of GST-pi also has been found in gastric cancer [40], malignant melanoma [41], lung cancer [42], breast cancer [43] and a range of other human tumors [44]. GST-pi has been up-regulated in transitional cell carcinoma of human urinary bladder [45]. Up-regulation of glutathione – related genes and enzyme activities has been found in cultured human cells by sub lethal concentration of inorganic arsenic [46]. There is evidence that arsenic induces DNA damage via the production of ROS (reactive oxygen species) [47]. GST-pi may be over-expressed in the urinary bladder to protect cells against arsenic-induced oxidative stress. Liver In the livers of hamsters exposed to sodium arsenite, ornithine amino transferase was over-expressed, senescence marker protein 30 was under-expressed, and fatty acid binding protein was under-expressed. Ornithine amino transferase has been found in the mitochondria of many different mammalian tissues, especially liver, kidney, and small intestine [48]. Ornithine amino transferase knockdown inhuman cervical carcinoma and osteosarcoma cells by RNA interference blocks cell division and causes cell death [49]. It has been suggested that ornithine amino transferase has a role in regulating mitotic cell division and it is required for proper spindle assembly in human cancer cells [49]. Senescence marker protein-30 (SMP30) is a unique enzyme that hydrolyzes diisopropylphosphorofluoridate. SMP30, which is expressed mostly in the liver, protects cells against various injuries by stimulating membrane calcium-pump activity [50]. SMP30 acts to protect cells from apoptosis [51]. In addition it protects the liver from toxic agents [52]. The livers of SMP30 knockout mice accumulate phosphatidylethanolamine, cardiolipin, phosphatidyl-choline, phosphatidylserine, and sphingomyelin [53]. Liver fatty acid binding protein (L-FABP) also was down- regulated. Decreased liver fatty acid-binding capacity and altered liver lipid distribution hasbeen reported in mice lacking the L-FABP gene [54]. High levels of saturated, branched-chain fatty acids are deleterious to cells and animals, resulting in lipid accumulation and cytotoxicity. The expression of fatty acid binding proteins (including L-FABP) protected cells against branched-chain saturated fatty acid toxicity [55]. Limitations: we preferred to study the pronounced spots seen in DIGE gels. Other spots were visible but not as pronounced. Because of limited funds, we did not identify these others protein spots. In conclusion, urinary bladders of hamsters exposed to sodium arsenite had a decrease in the expression of transgelin and an increase in the expression of GST-pi protein. Under-expression of transgelin has been found in various cancer systems and may be associated with arsenic carcinogenicity [30-33). Inorganic arsenic exposure has resulted in bladder cancer as has been reported in the past [1]. Over-expression of GST-pi may protect cells against oxidative stress caused by arsenite. In the liver OAT was up regulated and SMP-30 and FABP were down regulated. These proteomic results may be of help to investigators studying arsenic carcinogenicity. The Superfund Basic Research Program NIEHS Grant Number ES 04940 from the National Institute of Environmental Health Sciences supported this work. Additional support for the mass spectrometry analyses was provided by grants from NIWHS ES06694, NCI CA023074 and the BIOS Institute of the University of Arizona. Acknowledgement The Author wants to dedicate this paper to the memory of his former supervisor Dr. H. VaskenAposhian who passed away in September 6, 2019. He was an emeritus professor of the Department of Molecular and Cellular Biology at the University of Arizona. This research work was done under his sole supervision and with his great contribution.I also would like to thanks Dr. George Tsapraills, Center of Toxicology, The University of Arizona for identification of proteins by MS. References NRC (National Research Council), Arsenic in Drinking Water, Update to the 1999 Arsenic in Drinking Water Report. National Academy Press, Washington, DC 2001. Hopenhayn-Rich, C.; Biggs, M. L.; Fuchs, A.; Bergoglio, R.; et al. Bladder cancer mortality with arsenic in drinking water in Argentina. Epidemiology 1996, 7, 117-124. Chen, C.J.; Chen, C. W.; Wu, M. M.; Kuo, T. L. Cancer potential in liver, lung, bladder, and kidney due to ingested inorganic arsenic in drinking water. J. Cancer. 1992, 66, 888-892. IARC (International Agency for Research on Cancer), In IARC monograph on the evaluation of carcinogenicity risk to humans? Overall evaluation of carcinogenicity: an update of IARC monographs 1-42 (suppl. 7), International Agency for Research on Cancer, Lyon, France, 1987, pp. 100-106. Rossman, T. G.; Uddin, A. N.; Burns, F. J. Evidence that arsenite acts as a cocarcinogen in skin cancer. Appl. Pharmacol. 2004, 198, 394 404. Smith, A. H.; Hopenhayn-Rich, C.; Bates, M. N.; Goeden, H. M.; et al. Cancer risks from arsenic in drinking water. Health Perspect. 1992, 97, 259-267. Aposhian, H. V.; Aposhian, M. M. Arsenic toxicology: five questions. Res. Toxicol. 2006, 19, 1-15. Goering, P. L.; Aposhian, H. V.; Mass, M. J.; Cebrián, M., et al. The enigma of arsenic carcinogenesis: role of metabolism. Sci. 1999, 49, 5-14. Waalkes, M. P.; Liu, J.; Ward, J. M.; Diwan, B. A. Mechanisms underlying arsenic carcinogenesis: hypersensitivity of mice exposed to inorganic arsenic during gestation. 2004, 198, 31-38. Aposhian, H. V.; Gurzau, E. S.; Le, X. C.; Gurzau, A.; et al. Occurrence of monomethylarsonous acid in urine of humans exposed to inorganic arsenic. Res. Toxicol. 2000, 13, 693-697. Del Razo, L. M.; Styblo, M.; Cullen, W. R.; Thomas, D. J. Determination of trivalent methylated arsenicals in biological matrices. Appl. Pharmacol. 2001, 174, 282-293. Styblo, M.; Drobna, Z.; Jaspers, I.; Lin, S.; Thomas, D. J.; The role of biomethylation in toxicity and carcinogenicity of arsenic: a research update. Environ. Health Perspect. 2002, 5, 767-771. Chowdhury, U. K.; Zakharyan, R. A.; Hernandez, A.; Avram, M. D.; et al. Glutathione-S-transferase-omega [MMA(V) reductase] knockout mice: Enzyme and arsenic species concentrations in tissues after arsenate administration. Appl. Pharmaol. 2006, 216, 446-457. Kitchin, K. T. Recent advances in arsenic carcinogenesis: modes of action, animal model systems, and methylated arsenic metabolites. Appl. Pharmacol. 2001, 172, 249-261. Beckman, G.; Beckman, L.; Nordenson, I. Chromosome aberrations in workers exposed to arsenic. Health Perspect. 1977, 19, 145-146. Yamanaka, K.; Hoshino, M.; Okanoto, M.; Sawamura, R.; et al. Induction of DNA damage by dimethylarsine, a metabolite of inorganic arsenics, is for the major part likely due to its peroxyl radical. Biophys. Res. Commun. 1990, 168, 58-64. Yamanaka, K.; Okada, S. Induction of lung-specific DNA damage by metabolically methylated arsenics via the production of free radicals. Health Perspect. 1994, 102, 37-40. Simeonova, P. P.; Luster, M. I. Mechanisms of arsenic carcinogenicity:Genetic or epigenetic mechanisms? Environ. Pathol. Toxicol. Oncol. 2000, 19, 281-286. Popovicova, J.; Moser, G. J.; Goldsworthy, T. L.; Tice, R. R, Carcinogenicity and co-carcinogenicity of sodium arsenite in p53+/- male mice. 2000, 54, 134. Li, J. H.; Rossman, T. G. Mechanism of co-mutagenesis of sodium arsenite with N-methyl-N-nitrosourea. Trace Elem. 1989, 21, 373-381. Zhao, C. Q.; Young, M. R.; Diwan, B. A.; Coogan, T. P.; et al. Association of arsenic-induced malignant transformation with DNA hypomethylation and aberrant gene expression. Proc. Natl. Acad. Sci. USA, 1997, 94, 10907-10912. Abernathy, C. O.; Lui, Y. P.; Longfellow, D.; Aposhian, H. V.; et al. Arsenic: Health effects, mechanisms of actions and research issues. Health Perspect. 1999, 107, 593-597. Lee, T. C.; Tanaka, N.; Lamb, P. W.; Gilmer, T. M.; et al. Induction of gene amplification by arsenic. 1988, 241, 79-81. Lantz, R. C.; Lynch, B. J.; Boitano, S.; Poplin, G. S.; et al. Pulmonary biomarkers based on alterations in protein expression after exposure to arsenic. Health Perspect. 2007, 115, 586-591. Bradford, M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Biochem. 1976, 72, 248-254. Chowdhury, U. K.; Aposhian, H. V. Protein expression in the livers and urinary bladders of hamsters exposed to sodium arsenite. N. Y. Acad. Sci. 2008, 1140, 325-334. Andon, N. L.; Hollingworth, S.; Koller, A.; Greenland, A. J.; et al. Proteomic characterization of wheat amyloplasts using identification of proteins by Tandem Mass Spectrometry. 2002, 2, 1156-1168. Shapland, C.; Hsuan, J. J.; Totty, N. F.; Lawson, D. Purification and properties of transgelin: a transformation and shape change sensitive actin-gelling protein. Cell Biol. 1993, 121, 1065-1073. Lawson, D.; Harrison, M.; Shapland, C. Fibroblast transgelin and smooth muscle SM22 alpha are the same protein, the expression of which is down-regulated in may cell lines. Cell Motil. Cytoskeleton. 1997, 38, 250-257. Yang, Z.; Chang, Y- J.; Miyamoto, H.; Ni, J.; et al. Transgelin functions as a suppressor via inhibition of ARA54-enhanced androgen receptor transactivation and prostate cancer cell grown. Endocrinol. 2007, 21, 343-358. Yeo, M.; Kim, D- K.; Park, H. J.; Oh, T. Y.; et al. Loss of transgelin in repeated bouts of ulcerative colitis-induced colon carcinogenesis. 2006, 6, 1158-1165. Kim, H- J.; Sohng, I.; Kim, D- H.; Lee, D- C.; et al. Investigation of early protein changes in the urinary bladder following partial bladder outlet obstruction by proteomic approach. Korean Med. Sci. 2005, 20, 1000-1005. Shields, J. M.; Rogers-Graham, K.; Der, C. J. Loss of transgelin in breast and colon tumors and in RIE-1 cells by Ras deregulation of gene expression through Raf-independent pathways. Biol. Chem. 2002, 277, 9790-9799. Zeiden, A.; Sward, K.; Nordstrom, J.; Ekblad, E.; et al. Ablation of SM220c decreases contractility and actin contents of mouse vascular smooth muscle. FEBS Lett. 2004, 562, 141-146. Hoivik, D.; Wilson, C.; Wang, W.; Willett, K.; et al. Studies on the relationship between estrogen receptor content, glutathione S-transferase pi expression, and induction by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin and drug resistance in human breast cancer cells. Biochem. Biophys. 1997, 348, 174-182. Hayes, J. D.; Pulford. D. J. The glutathione S-transferase super gene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Critical Rev. Biochem. Mol. Biol. 1995, 30, 445-600. Zhao, T.; Singhal, S. S.; Piper, J. T.; Cheng, J.; et al. The role of human glutathione S-transferases hGSTA1-1 and hGSTA2-2 in protection against oxidative stress. Biochem. Biophys. 1999, 367, 216-224. Zakharyan, R. A.; Sampayo-Reyes, A.; Healy, S. M.; Tsaprailis, G.; et al. Human monomethylarsonic acid (MMA) reductase is a member of the glutathione-S-transferase superfamily. Res. Toxicol. 2001, 14, 1051-1057. Tsuchida, S.; Sekine, Y.; Shineha, R.; Nishihira, T.; et al. Elevation of the placental glutathione S-transferase form (GST-PI) in tumor tissues and the levels in sera of patients with cancer. Cancer Res. 1989, 43, 5225-5229. Tsutsumi, M.; Sugisaki, T.; Makino, T.; Miyagi, N.; et al. Oncofetal expression of glutathione S-transferase placental form in human stomach carcinomas. Gann. 1987, 78, 631-633. Mannervik, B.; Castro, V. M.; Danielson, U. H.; Tahir, M. K.; et al. Expression of class Pi glutathione transferase in human malignant melanoma cells. Carcinogenesis (Lond.). 1987, 8, 1929-1932. Di llio, C.; Del Boccio, G.; Aceto, A.; Casaccia, R.; et al. Elevation of glutathione transferase activity in human lung tumor. Carcinogenesis (Lond.). 1988, 9, 335-340. Sreenath, A. S.; Ravi, K. K.; Reddy, G. V.; Sreedevi, B.; et al. Evidence for the association of synaptotagmin with glutathione S- transferase: implications for a novel function in human breast cancer. Clinical Biochem. 2005, 38, 436-443. Shea, T. C.; Kelley S. L.; Henner, W. D. Identification of an anionic form ofglutathione transferase present in many human tumors and human tumor cell lines. Cancer Res. 1988, 48, 527-533. Simic, T.; Mimic-Oka, J.; Savic-Radojevic, A.; Opacic, M.; et al. Glutathione S- transferase T1-1 activity upregulated in transitional cell carcinoma of urinary bladder. 2005, 65, 1035-1040. Schuliga, M.; Chouchane, S.; Snow, E. T. Up-regulation of glutathione - related genes and enzyme activities in cultured human cells by sub-lethal concentration of inorganic arsenic. Sci. 2002, 70, 183-192. Matsui, M.; Nishigori, C.; Toyokuni, S.; Takada, J.; et al. The role of oxidative DNA damage in human arsenic carcinogenesis: detection of 8 hydroxy-2'-deoxyguanosine in arsenic-related Bowen's disease. Invest. Dermatol. 1999, 113, 26-31. Sanada, Y.; Suemori, I.; Katunuma, N. Properties of ornithine aminotransferase from rat liver, kidney, and small intestine. Biophys. Acta. 1970, 220, 42-50. Wang, G.; Shang, L.; Burgett, A. W. G.; Harran, P. G.; et al. Diazonamide toxins reveal an unexpected function for ornithine d-amino transferase in mitotic cell division. PNAS, 2007, 104, 2068-2073. Fujita, T.; Inoue, H.; Kitamura, T.; Sato, N.; et al. Senescence marker protein-30 (SMP30) rescues cell death by enhancing plasma membrane Caat-pumping activity in hep G2 cells. Biophys. Res. Commun. 1998, 250, 374-380. Ishigami, A.; Fujita, T.; Handa, S.; Shirasawa, T.; et al. Senescence marker protein-30 knockout mouse liver is highly susceptible to tumors necrosis factor-∞ and fas-mediated apoptosis. J. Pathol. 2002, 161, 1273-1281. Kondo, Y.; Ishigami, A.; Kubo, S.; Handa, S.; et al. Senescence marker protein-30is a unique enzyme that hydrolyzes diisopropylphosphorofluoridate in the liver. FEBS Letters. 2004, 570, 57-62. Ishigami, A.; Kondo, Y.; Nanba, R.; Ohsawa, T.; et al. SMP30 deficiency in mice causes an accumulation of neutral lipids and phospholipids in the liver and shortens the life span. Biophys. Res. Commun. 2004, 315, 575-580. Martin, G. G.; Danneberg, H.; Kumar, L. S.; Atshaves, B. P.; et al. Decreased liver fatty acid binding capacity and altered liver lipid distribution in mice lacking the liver fatty acid binding protein gene. Biol. Chem. 2003, 278, 21429-21438. Atshaves, B. P.; Storey, S. M.; Petrescu, A.; Greenberg, C. C.; et al. Expression of fatty acid binding proteins inhibits lipid accumulation and alters toxicity in L cell fibroblasts. J. Physiol. Cell Physiol. 2002, 283, C688-2703.

Devido a evolução dos sistemas cerâmicos, atualmente, são permitidas inúmeras associações de materiais restauradores. Entretanto, mimetizar as características visuais das próteses livres de metal com próteses metalocerâmica permanece um desafio. Assim, o objetivo deste trabalho foi relatar o caso clínico de substituição de próteses desadaptadas, homogeneizando próteses parciais fixas livres de metal e metalocerâmicas dento e implantossuportadas, após cirurgia periodontal. O paciente de 55 anos, gênero masculino, compareceu à Clínica de Prótese Parcial Fixa, da Faculdade de Odontologia de Araçatuba – FOA/UNESP em 2017 queixando-se dos seus dentes anteriores estarem “feios” e com a “raiz aparecendo”. No exame clínico foi constatada recessão gengival nos elementos 11 e 13, os quais continham uma prótese parcial fixa de três elementos metalocerâmicas desadaptada, prótese sobre implante nos elementos 21 e 22, que apresentavam-se desadaptadas e com estética desfavorável. No exame radiográfico pôde-se observar a presença de um núcleo metálico fundido nos elementos 11 e 13. Foi proposto, então, a confecção de coroas totais livres de metal nos elementos anteriores superiores (13, 12, 11, 21 e 23) e coroa metalocerâmica para o elemento 22. Para isso, foi realizado enceramento diagnóstico inicial e, após o consentimento do paciente, foi efetuada a remoção das próteses antigas. Com a remoção, visualizamos uma depressão na vestibular do rebordo na área do 12 (ausente), solucionado com um enxerto gengival subepitelial. Após a realização dos preparos dentários e moldagem com silicone de adição as peças foram confeccionadas com sistema e.max Ceram (Ivoclar Vivadent). Após a prova estética e ajustes oclusais iniciais, as peças foram preparadas para cimentação resinosa com o sistema Variolink® N (Ivoclar Vivadent). O cimento transparente foi selecionado previamente por meio de provas com o kit Try-In. Conclui-se que, apesar da disparidade dos materiais, é possível mimetizá-los e oferecer resultados reabilitadores suficientemente estéticos e satisfatórios.Descritores: Implantação Dentária; Estética Dentária; Materiais Dentários.ReferênciasCardenas AFM, Mora CAP, Siqueira FSF, Parreiras SO, Gomes JC. Restabelecimento estético de um sorriso envelhecido: Caso clínico. Revista APCD de Estética. 2015;3(1):42-52.Strasding M, Fehmer V, Pjetursson BE, Sailer I. Extending the service life of existing dental restorations with esthetic and functional limitations. J Prosthet Dent. 2018;119(6):893-96.Koidou VP, Rosenstiel SF, Rashid RG. Celebrity smile esthetics assessment: Smile angulation. J Prosthet Dent, 2017;117(5):636-41.Levin, EI. Dental esthetics and the golden proportion. J Prosthet Dent. 1978;40(3):244-52.Flores-Mir C, Silva E, Barriga MI, Lagravere MO, Major PW. Lay person's perception of smile aesthetics in dental and facial views. J Orthod. 2004;31(3):204-9.Cotrim, ER, Vasconcelos Júnior, ÁV, Haddad, ACSS, Reis SAB. Perception of adults' smile esthetics among orthodontists, clinicians and laypeople. Dental Press J. Orthod. 2015;20(1):40-4.Chaudhari A, Bagga DK, Agrawal P, Kalra H, Sirohi D. An assessment of the self-satisfying smile among different professionals. J Int Oral Health. 2018;10(3):111-14.Papaspyridakos P, Chen CJ, Singh M, Weber HP, Gallucci GO. Success criteria in implant dentistry: a systematic review. J Dent Res. 2012;91(3):242-48.Bonfante EA, Suzuki M, Lorenzoni FC, Sena LA, Hirata R, Bonfante G et al. Probability of survival of implant-supported metal ceramic and CAD/CAM resin nanoceramic crowns. Dent Mater J. 2015;31(8):e168-77.Egilmez F, Ergun G, Cekic-Nagas I, Bozkaya S. Implant-supported hybrid prosthesis: conventional treatment method for borderline cases. Eur J Dent. 2015;9(3):442-48.Schweitzer DM, Goldstein GR, Ricci JL, Silva NR, Hittelman EL. Comparison of bond strength of a pressed ceramic fused to metal versus feldspathic porcelain fused to metal. J Prosthodont. 2005;14(4):239-47.Venkatachalam B, Goldstein GR, Pines MS, Hittelman EL. Ceramic pressed to metal versus feldspathic porcelain fused to metal: a comparative study of bond strength. Int J Prosthodont. 2009;22(1):94-100.Holden JE, Goldstein GR, Hittelman EL, Clark EA. Comparison of the marginal fit of pressable ceramic to metal ceramic restorations. J Prosthodont. 2009;18: 645-48.Sinhori BS, de Andrada MAC, Lopes GC, Monteiro Junior S, Baratieri LN. Influence of Teeth Preparation Finishing on the Adaptation of Lithium Disilicate Crowns. Int J Biomater. 2017; ID 2078526.Hoppen LRC, Garbin CA, Rigo L, Schuh C, FederizzI L. Comparação estética entre coroas confeccionadas com os sistemas Cubo e metalocerâmico. Rev Sul-Bras Odontol. 2010;7(2):146-53.Mazur CE, Machado CT, Malheiros Pfau VJ, Augusto Pfau, E. Planejamento multidisciplinar na reconstrução do sorriso. JCDR. 2017; 14(2):62-70.Zuhr O, Bäumer D, Hürzeler M The addition of soft tissue replacement grafts in plastic periodontal and implant surgery: critical elements in design and execution. J Clin Periodontol. 2014;41(Suppl15):S123-42.Consolaro A. Saucerização: um mecanismo natural de adaptação peri-implantar cervical. Dental Press Implantol, 2014;8(4):8-15.Nealon FH. Acrylic restorations by the operative nonpressure procedure. J Prosthet Dent. 1952;2(4):513-27.Newman MG, Takei H, Klokkevold PR, Carranza FA. Periodontia clínica. Rio de Janeiro: Elsevier Brasil; 2007. p.926-35.Raetzke PB. Covering localized areas of root exposure employing the “envelope” technique. J Periodontol. 1985;56(7):397-402.Hannah R, Ramani P, Sherlin HJ, Ranjith G, Ramasubramanian A, Jayaraj G et al. Awareness about the use, ethics and scope of dental photography among undergraduate dental students dentist behind the lens. RJPT. 2018; 11(3):1012-16.Alberton SB, Alberton V, Carvalho RV. Providing a harmonious smile with laminate veneers for a patient with peg-shaped lateral incisors. J Conserv Dent. 2017;20(3):210-13.Vervaeke S, Matthys C, Nassar R, Christiaens V, Cosyn J, De Bruyn H. Adapting the vertical position of implants with a conical connection in relation to soft tissue thickness prevents early implant surface exposure: A 2‐year prospective intra‐subject comparison. J Clin Periodontol. 2018;45(5):605-12.Anusavice K, Shen C, Rawls HR. Dental casting and soldering alloys. In: Anusavice KJ, Phillips’ Science of Dental Material. St. Louis: Elsevier; 2003. p.563–620.Arinc H. Implant-supported fixed partial prostheses with different prosthetic materials: a three-dimensional finite element stress analysis. Implant Dent. 2018;27(3):303-10.Monnet-Corti V, Antezack A, Pignoly M. Comment parfaire l’esthétique du sourire: toujours en rose! Orthod Fr. 2018;89(1):71-80.Tonetti MS, Cortellini P, Graziani F, Cairo F, Lang NP, Abundo R et al. Immediate versus delayed implant placement after anterior single tooth extraction: the timing randomized controlled clinical trial. J Clin Periodontol. 2017;44(2):215-24.Pradeep AR, Karthikeyan BV. Peri-implant papilla reconstruction: Realities and limitations. J Periodontol. 2006; 77(3):534-44.Nariman RH, Pai UY, Soumya MK, Hegde R. A clinical assessment of the volume of interproximal papilla after definitive prosthesis around immediate and delayed loading implants placed in the maxillary esthetic zone: An in vivo study. J Indian Prosthodont Soc. 2018;18(2):168-73.Neves FDD, Silveira-Júnior CD, Coró V, Silva-Neto JP, Simamoto-Júnior PC, Prado CJD. Gingival conditioning in an implant-supported prosthesis: a clinical report. J Oral Implantol. 2013;39(4):483-85.

Background: Reperfusion following ischemia can lead to more injuries than ischemia itself especially in diabetic patients. The aim of this study was to evaluate the effect of dexmedetomidine on ischemia-reperfusion injury (IRI) in rats with have hepatic IRI and diabetes mellitus. Methodology: Twenty-eight Wistar Albino rats were randomised into four groups as control (C), diabetic (DC), diabetic with hepatic ischemia-reperfusion injury (DIR), and diabetic but administered dexmedetomidine followed by hepatic IRI (DIRD) groups. Hepatic tissue samples were evaluated histopathologically by semiquantitative methods. Malondialdehyde (MDA), superoxide dismutase (SOD), glutathion s-transpherase (GST), and catalase (CAT) enzyme levels were investigated in liver and kidney tissues as oxidative state parameters. Results: In Group DIR; hepatocyte degeneration, sinusoidal dilatation, pycnotic nucleus, and necrotic cells were found to be more in rat hepatic tissue; while mononuclear cell infiltration was higher in the parenchyme. MDA levels were significantly lower; but SOD levels were significantly higher in Group DIRD with regard to Group DIR. In the IRI induced diabetic rats’ hepatic and nephrotic tissues MDA levels, showing oxidative injury, were found to be lower. SOD levels, showing early antioxidant activity, were higher. Conclusion: The enzymatic findings of our study together with the hepatic histopathology indicate that dexmedetomidine has a potential role to decrease IRI. Key words: Hepatic ischemia reperfusion injury; Diabetes mellitus; Dexmedetomidine; Rat; MDA; SOD Citation: Sezen SC, Işık B, Bilge M, Arslan M, Çomu FM, Öztürk L, Kesimci E, Kavutçu M. Effect of dexmedetomidine on ischemia-reperfusion injury of liver and kidney tissues in experimental diabetes and hepatic ischemia-reperfusion injury induced rats. Anaesth Pain & Intensive Care 2016;20(2):143-149 Received: 21 November 2015; Reviewed: 10, 24 December 2015, 9, 10 June 2016; Corrected: 12 December 2015; Accepted: 10 June 2016 INTRODUCTİON Perioperative acute tissue injury induced by ischemia-reperfusion is a comman clinical event caused by reduced blood supply to the tissue being compromised during major surgery. Ischemia leads to cellular injury by depleting cellular energy deposits and resulting in accumulation of toxic metabolites. The reperfusion of tissues that have remained in ischemic conditions causes even more damage.1 Furthermore hepatic ischemia-reperfusion injury (IRI) demonstrates a strong relationship with peri-operative acute kidney injury.2 The etiology of diabetic complications is strongly associated with increased oxidative stress (OS). Diabetic patients are known to have a high risk of developing OS or IRI which results with tissue failure.3 The most important role in ischemia and reperfusion is played by free oxygen radicals.1 In diabetes, characterized by hyperglycemia, even more free oxygen radicals are produced due to oxidation of glucose and glycosylation of proteins.3 The structures which are most sensitive to free oxygen radicals in the cells are membrane lipids, proteins, nucleic acids and deoxyribonucleic acids.1 It has been reported that endogenous antioxidant enzymes [superoxide dismutase (SOD), glutathion s-transpherase (GST), catalase (CAT)] play an important role to alleviate IRI.4-8 Also some pharmacological agents have certain effects on IRI.1 The anesthetic agents influence endogenous antioxidant systems and free oxygen radical formation.9-12 Dexmedetomidine is a selective α-2 adrenoceptor agonist agent. It has been described as a useful and safe adjunct in many clinical applications. It has been found that it may increase urine output by considerably redistributing cardiac output, inhibiting vasopressin secretion and maintaining renal blood flow and glomerular filtration. Previous studies demonstrated that dexmedetomidine provides protection against renal, focal cerebral, cardiac, testicular, and tourniquet-induced IRI.13-18 Arslan et al observed that dexmedetomidine protected against lipid peroxidation and cellular membrane alterations in hepatic IRI, when given before induction of ischemia.17 Si et al18 demonstrated that dexmedetomidine treatment results in a partial but significant attenuation of renal demage induced by IRI through the inactivation of JAK/STAT signaling pathway in an in vivo model. The efficacy of the dexmedetomidine for IRI in diabetic patient is not resarched yet. The purpose of this experimental study was to evaluate the biochemical and histological effects of dexmedetomidine on hepatic IRI in diabetic rat’s hepatic and renal tissue. METHODOLOGY Animals and Experimental Protocol: This study was conducted in the Physiology Laboratory of Kirikkale University upon the consent of the Experimental Animals Ethics Committee of Kirikkale University. All of the procedures were performed according to the accepted standards of the Guide for the Care and Use of Laboratory Animals. In the study, 28 male Wistar Albino rats, weighing between 250 and 300 g, raised under the same environmental conditions, were used. The rats were kept under 20-21 oC at cycles of 12-hour daylight and 12-hour darkness and had free access to food until 2 hours before the anesthesia procedure. The animals were randomly separated into four groups, each containing 7 rats. Diabetes was induced by a single intraperitoneal injection of streptozotocin (Sigma Chemical, St. Louis, MO, USA) at a dose of 65 mg/kg body weight. The blood glucose levels were measured at 72 hrs following this injection. Rats were classified as diabetic if their fasting blood glucose (FBG) levels exceeded 250 mg/dl, and only animals with FBGs of > 250 mg/dl were included in the diabetic groups (dia­betes only, diabetes plus ischemia-reperfusion and diabetes plus dexmedetomidine-ischemia-reperfusion). The rats were kept alive 4 weeks after streptozotocin injection to allow development of chronic dia­betes before they were exposed to ischemia-reperfusion.(19) The rats were weighed before the study. Rats were anesthetized with intraperitoneal ketamine 100 mg/kg. The chest and abdomen were shaved and each animal was fixed in a supine position on the operating table. The abdomen was cleaned with 1% polyvinyl iodine and when dry, the operating field was covered with a sterile drape and median laparotomy was performed. There were four experimental groups (Group C (sham-control; n = 7), (Group DC (diabetes-sham-control; n = 7), Group DIR (diabetes-ischemia-reperfusion; n = 7), and Group DIRD (diabetes-ischemia-reperfusion-dexmedetomidine; n = 7). Sham operation was performed on the rats in Group C and Group DC. The sham operation consisted of mobilization of the hepatic pedicle only. The rats in this group were sacrificed 90 min after the procedure. Hepatic I/R injury was induced in Groups DIR and DIRD respectively with hepatic pedicle clamping using a vascular clamp as in the previous study of Arslan et al.(17) After an ischemic period of 45 min, the vascular clamp was removed. A reperfusion period was maintained for 45 min. In Group DIRD, dexmedetomidine hydrochloride 100 μg/kg, (Precedex 100 μg/2 ml, Abbott®, Abbott Laboratory, North Chicago, Illinois, USA) was administrated via intraperitoneal route 30 minutes before surgery. All the rats were given ketamine 100 mg/kg intraperitoneally and intracardiac blood samples were obtained. Preserving the tissue integrity by avoiding trauma, liver and renal biopsy samples were obtained. Biochemical Analysis: The liver and renal tissues were first washed with cold deionized water to discard blood contamination and then homogenized in a homogenizer. Measurements on cell contest require an initial preparation of the tissues. The preparation procedure may involve grinding of the tissue in a ground glass tissue blender using a rotor driven by a simple electric motor. The homogenizer as a tissue blender similar to the typical kitchen blender is used to emulsify and pulverize the tissue (Heidolph Instruments GMBH & CO KGDiax 900 Germany®) at 1000 U for about 3 min. After centrifugation at 10,000 g for about 60 min, the upper clear layer was taken. MDA levels were determined using the method of Van Ye et al,(20) based on the reaction of MDA with thiobarbituric acid (TBA). In the TBA test reaction, MDA and TBA react in acid pH to form a pink pigment with an absorption maximum at 532 nm. Arbitrary values obtained were compared with a series of standard solutions (1,1,3,3-tetraethoxypropane). Results were expressed as nmol/mg.protein. Part of the homogenate was extracted in ethanol/chloroform mixture (5/3 v/v) to discard the lipid fraction, which caused interferences in the activity measurements of T-SOD, CAT and GST activities. After centrifugation at 10.000 x g for 60 min, the upper clear layer was removed and used for the T-SOD, CAT, GST enzyme activity measurement by methods as described by Durak et al21, Aebi22 and Habig et al23 respectively. One unit of SOD activity was defined as the enzyme protein amount causing 50% inhibition in NBTH2 reduction rate and result were expressed in U/mg protein. The CAT activity method is based on the measurement of absorbance decrease due to H2O2 consumption at 240 nm. The GST activity method is based on the measurement of absorbance changes at 340 nm due to formation of GSH-CDNB complex. Histological determinations: Semiquantitative evaluation technique used by Abdel-Wahhab et al(24) was applied for interpreting the structural changes investigated in hepatic tissues of control and research groups. According to this, (-) (negative point) represents no structural change, while (+) (one positive point) represents mild, (++) (two positive points) medium and (+++) (three positive points) represents severe structural changes. Statistical analysis: The Statistical Package for the Social Sciences (SPSS, Chicago, IL, USA) 20.0 softwre was used for the statistical analysis. Variations in oxidative state parameters, and histopathological examination between study groups were assessed using the Kruskal-Wallis test. The Bonferroni-adjusted Mann-Whitney U-test was used after significant Kruskal-Wallis to determine which groups differed from the others. Results were expressed as mean ± standard deviation (Mean ± SD). Statistical significance was set at a p value < 0.05 for all analyses. RESULTS There was statistically significant difference observed between the groups with respect to findings from the histological changes in the rat liver tissue (hepatocyte degeneration, sinüsoidal dilatation, pycnotic nucleus, prenecrotic cell) determined by light microscopy according to semiquantitative evaluation techniques (p < 0.0001). In Group DIR, hepatocyte degeneration was significantly high compared to Group C, Group DC and Group DIRD (p < 0.0001, p < 0.0001, p = 0.002, respectively), (Table 1, Figure 1-4). Similarly, sinüsoidal dilatation was significantly higher in Group DIR (p < 0.0001, p = 0.004, p = 0.015, respectively). Although, pcynotic nucleus was decreased in Group DIRD, it did not make a significant difference in comparison to Group DIR (p = 0.053), (Table 1, Figure 1-4). The prenecrotic cells were significantly increased in Group DIR, with respect to Group C, Group DC and Group DIRD (p < 0.0001, p = 0.004, p < 0.0001, respectively), (Table 1, Figure 1-4). Table 1. The comparison of histological changes in rat hepatic tissue [Mean ± SD)] p**: Statistical significance was set at a p value < 0.05 for Kruskal-Wallis test *p < 0.05: When compared with Group DIR Figure 1: Light microscopic view of hepatic tissue of Group C (control). VC: vena centralis, *: sinusoids. ®: hepatocytes, k: Kupffer cells, G: glycogen granules, mc: minimal cellular changes, Hematoxilen & Eosin x 40 Figure 2: Light-microscopic view of hepatic tissue of Group DC (diabetes mellitus control) (G: Glycogen granules increased in number, (VC: vena centralis, *:sinusoids. ®:hepatocytes, k:Kupffer cells, G: glycogen granules, mc: minimal cellular changes; Hematoxylin & Eosin x 40) Figure 3: Light-microscopic view of hepatic tissue of Group DIR (Diabetes Mellitus and ischemia-reperfusion) (VC: vena centralis, (H) degenerative and hydrophic hepatocytes, (dej) vena centralis degeneration (centrolobar injury) (*): sinusoid dilatation. (←) pycnotic and hyperchromatic nuclei, MNL: mononuclear cell infiltration, (¯) congestion, K: Kupffer cell hyperplasia, (­) vacuolar degeneration (Hematoxylin & Eosin x 40) Figure 4: Light-microscopic view of hepatic tissue of Group DIRD (Diabetes Mellitus and ischemia-reperfusion together with dexmedetomidine applied group) (VC: vena centralis, (MNL) mononuclear cell infiltration, (dej) hydrophilic degeneration in hepatocytes around vena centralis, (conj) congestion, G: glycogen granules, (←) pycnotic and hyperchromatic nuclei, sinusoid dilatation (*) (Hematoxylin & Eosin x 40) Besides, in liver tissue parenchyma, MN cellular infiltration was a light microscopic finding; and showed significant changes among the groups (p < 0.0001). This was significantly higher in Group DIR, compared to Group C, DC, and DIRD (p < 0.0001, p=0.007, p = 0.007, respectively), (Table 1, Figure 1-4). The enzymatic activity of MDA, SOD and GST in hepatic tissues showed significant differences among the groups [(p = 0.019), (p = 0.034). (p = 0.008) respectively]. MDA enzyme activity was significantly incresed in Group DIR, according to Group C and Group DIRD (p = 0.011, p = 0.016, respectively), (Table 2). In Group DIR SOD enzyme activity was lower with respect to Group C and Group DIRD (p = 0.010, p = 0.038, respectively), (Table 2). The GST enzyme activity was significantly higher in Group DIR, when compared to Group C, DC and DIRD (p = 0.007, p = 0.038, p = 0.039, respectively), (Table 2). Table 2. Oxidative state parameters in rat hepatic tissue [Mean ± SD] p**: Statistical significance was set at a p value < 0.05 for Kruskal-Wallis test *p < 0.05: When compared with Group DIR The enzymatic activity of MDA, SOD in renal tissues, showed significant differences among the groups [(p < 0.0001), (p = 0.008) respectively ]. MDA enzyme activity was significantly incresed in Group DIR, according to Group C and Group DIRD (p < 0.0001, p < 0.0001, respectively). Also MDA enzyme activity level was significantly increased in Group DC, in comparison to Group C and Group DIRD (p = 0.003, p = 0.001, respectively), (Table 3). In Group DIR SOD enzyme activity was lower with respect to Group C and Group DIRD (p = 0.032, p = 0.013, respectively), (Table 3). The GST enzyme activity was significantly higher in Group DIR than the other three groups, however; CAT levels were similar among the groups (Table 3). Table 3: Oxidative state parameters in rat nephrotic tissue [Mean ± SD)] p**: Statistical significance was set at a p value < 0.05 for Kruskal-Wallis test *p < 0.05: When compared with Group DIR DISCUSSION In this study, we have reported the protective effect of dexmedetomidine in experimental hepatic and renal IRI model in the rat by investigating the MDA and SOD levels biochemically. Besides, hepatic histopathological findings also supported our report. Ischemic damage may occur with trauma, hemorrhagic shock, and some surgical interventions, mainly hepatic and renal resections. Reperfusion following ischemia results in even more injury than ischemia itself. IRI is an inflammatory response accompanied by free radical formation, leucocyte migration and activation, sinusoidal endothelial cellular damage, deteoriated microcirculation and coagulation and complement system activation.1 We also detected injury in hepatic and renal tissue caused by reperfusion following ischemia in liver. Experimental and clinical evidence indicates that OS is involved in both the pathogenesis and the complications of diabetes mellitus.25,26 Diabetes mellitus is a serious risk factor for the development of renal and cardiovascular disease. It is also related to fatty changes in the liver.27 Diabetes-related organ damage seems to be the result of multiple mechanisms. Diabetes has been associated with increased free radical reactions and oxidant tissue damage in STZ-induced diabetic rats and also in patients.26Oxidative stress has been implicated in the destruction of pancreatic β-cells28 and could largely contribute to the oxidant tissue damage associated with chronic hyperglycemia.29 A number of reports have shown that antioxidants can attenuate the complications of diabetes in patients30 and in experimental models.28,31 This study demonstrated that diabetes causes a tendency to increase the IRI. There is a lot of investigations related to the pharmacological agents or food supplements applied for decreasing OS and IRI. Antioxidant agents paly an important role in IRI by effecting antioxidant system or lessening the formation of ROS. It has been reported that anesthetic agents too, are effective in oxidative stress.1 During surgical interventions, it seems rational to get benefit from anesthetic agents in prevention of OS caused by IRI instead of using other agents. It has been declared that; dexmedetomidine; as an α-2 agonist with sedative, hypnotic properties; is important in prevention of renal, focal, cerebral, cardiac, testicular and tourniquet-induced IRI.13-18 On the other hand Bostankolu et al. concluded that dexmedetomidine did not have an additional protective role for tournique induced IRI during routine general anesthesia.32 In this study; we have shown that dexmedetomidine has a reducing effect in IRI in diabetic rats. Some biochemical tests and histopathological evaluations are applied for bringing up oxidative stress and IRI in the tissues. Reactive oxygen species (ROS) that appear with reperfusion injury damage cellular structures through the process of the lipid peroxidation of cellular membranes and yield toxic metabolites such as MDA.33 As an important intermidiate product in lipid peroxidation, MDA is used as a sensitive marker of IRI.34 ROS-induced tissue injury is triggered by various defense mechanisms.35 The first defence mechanisms include the antioxidant enzymes of SOD, CAT, and GPx. These endogenous antioxidants are the first lines of defence against oxidative stres and act by scavenging potentially damaging free radical moieties.36 There is a balance between ROS and the scavenging capacity of antioxidant enzymes.1-8 In this study, for evaluation of oxidative damage and antioxidant activity, MDS, SOD, GST and CAT levels were determined in liver and kidney tissues. MDA levels in hepatic and renal tissues were higher in Group DIR compared to Group C and Group DIRD. GST levels were higher in Group DIR compared to all the other three groups. When the groups were arranged from highest to lowest order, with respect to CAT levels, the order was; Group DIR, Group DIRD, Group DC and Group C. However, the difference was not significant. The acute phase reactant MDA, as a marker of OS, was found to be high in Group DIR and low in Group DIRD. This could be interpreted as the presence of protective effect of dexmedetomidine in IRI. IRI developing in splanchnic area causes injury also in the other organs.35 Leithead et al showed that clinically significant hepatic IRI demonstrates a strong relationship with peri-operative acute kidney injury.2 In our experimental research that showed correlation to that of research by Leithead et al. After hepatic IRI in diabetic rats renal OS marker MDA levels were significantly more in Group DIR than Group DIRD. In our study, we observed histopathological changes in the ischemic liver tissue and alterations in the level of MDA, SOD, GST and CAT levels which are OS markers. Histopathological changes of the liver tissues are hepatocyt degeneration, sinusoidal dilatation, nuclear picnosis, celluler necrosis, mononuclear cell infiltrationat paranchimal tissue. These histopathological injury scores were significantly lower in the Group DIRD than those in group DIR. LIMITATION Study limitation is there was no negative control group, as this type of surgical intervention is not possible in rats without anesthesia. CONCLUSION The enzymatic findings of our study together with the hepatic histopathology indicate that dexmedetomidine has a potential role to decrease ischemia-reperfusion injury. Conflict of interest and funding: The authors have not received any funding or benefits from industry or elsewhere to conduct this study. Author contribution: ŞCS: Concept, conduction of the study work and manuscript editing; BI: the main author to write the article; MB & MK: biochemical analysis; MA: manuscript writing; FMÇ: helped us with experimental study; LÖ & EK: collection of data REFERENCES Collard CD, Gelman S. Pathophysiology, clinical manifestations, and prevention of ischemia-reperfusion injury. Anesthesiology. 2001;94(6):1133. [PubMed] [Free full text] Leithead JA, Armstrong MJ, Corbett C, Andrew M, Kothari C, Gunson BK, et al. Hepatic ischemia reperfusion injury is associated with acute kidney injury following donation after brain death liver transplantation. Transpl Int. 2013;26(11):1116. doi: 10.1111/tri.12175. [PubMed] [Free full text] Panés J, Kurose I, Rodriguez-Vaca D, Anderson DC, Miyasaka M, Tso P, et al. Diabetes exacerbates inflammatory responses to ischemia-reperfusion. Circulation. 1996;93(1):161. [PubMed] [Free full text] Touyz RM. Reactive oxygen species and angiotensin II signaling in vascular cells-implications in cardiovascular disease. Braz J Med Biol Res. 2004;37:1263. [PubMed] [Free full text] Olivares-Corichi IM, Ceballos G, Ortega-Camarillo C, Guzman-Grenfell AM, Hicks JJ. Reactive oxygen species (ROS) induce chemical and structural changes on human insulin in vitro, including alterations in its immunoreactivity. Front Biosci. 2005;10:834. [PubMed] Witko-Sarsat V, Friedlander M, Capeillere-Blandin C, Nguyen-Khoa T, Nguyen AT, Zingraff J, et al. Advanced oxidation protein products as a novel marker of oxidative stress in uremia. Kidney Int. 1996;49:1304. [PubMed] Harman D. Free radical theory of aging: An update: Increasing the functional life span. Ann N Y Acad Sci. 2006;1067:10. [PubMed] Nita DA, Nita V, Spulber S, Moldovan M, Popa DP, Zagrean AM, Zagrean L. Oxidative damage following cerebral ischemia depends on reperfusion – a biochemical study in rat. J Cell Mol Med. 2001;5:163–170. [PubMed] [Free full text] Annecke T, Kubitz JC, Kahr S, Hilberath JM, Langer K, Kemming GI, et al. Effects of sevoflurane and propofol on ischaemia-reperfusion injury after thoracic-aortic occlusion in pigs. Br J Anaesth. 2007;98(5):581. [PubMed] [Free full text] De Hert SG, Van der Linden PJ, Cromheecke S, Meeus R, Nelis A, Van Reeth V, ten Broecke PW, et al. Cardioprotective properties of sevoflurane in patients undergoing coronary surgery with cardiopulmonary bypass are related to the modalities of its administration. Anesthesiology. 2004;101(2):299. [PubMed] [Free full text] Yuzer H, Yuzbasioglu MF, Ciralik H, Kurutas EB, Ozkan OV, Bulbuloglu E, et al. Effects of intravenous anesthetics on renal ischemia/reperfusion injury. Ren Fail. 2009;31(4):290. [PubMed] [Free full text] Lee HT, Ota-Setlik A, Fu Y, Nasr SH, Emala CW. Differential protective effects of volatile anesthetics against renal ischemia-reperfusion injury in vivo. Anesthesiology. 2004;101(6):1313. [PubMed] [Free full text] Lai YC, Tsai PS, Huang CJ. Effects of dexmedetomidine on regulating endotoxin-induced up-regulation of inflammatory molecules in murine macrophages. J Surg Res. 2009;154(2):212. doi: 10.1016/j.jss.2008.07.010. [PubMed] Yoshitomi O, Cho S, Hara T, Shibata I, Maekawa T, Ureshino H, Sumikawa K. Direct protective effects of dexmedetomidine against myocardial ischemia-reperfusion injury in anesthetized pigs. Shock. 2012;38(1):92. doi: 10.1097/SHK.0b013e318254d3fb. [PubMed] Jolkkonen J, Puurunen K, Koistinaho J, Kauppinen R, Haapalinna A, Nieminen L, et al. Neuroprotection by the alpha2-adrenoceptor agonist, dexmedetomidine, in rat focal cerebral ischemia. Eur J Pharmacol. 1999;372(1):31. [PubMed] Kocoglu H, Ozturk H, Ozturk H, Yilmaz F, Gulcu N. Effect of dexmedetomidine on ischemia-reperfusion injury in rat kidney: a histopathologic study. Ren Fail. 2009;31(1):70. doi: 10.1080/08860220802546487. [PubMed] Arslan M, Çomu FM, Küçük A, Öztürk L, Yaylak F. Dexmedetomidine protects against lipid peroxidation and erythrocyte deformability alterations in experimental hepatic ischemia reperfusion injury. Libyan J Med. 2012;7. doi: 10.3402/ljm.v7i0.18185 [PubMed] [Free full text] Si Y, Bao H, Han L, Shi H, Zhang Y, Xu L, et al. Dexmedetomidine protects against renal ischemia and reperfusion injury by inhibiting the JAK/STAT signaling activation. J Transl Med. 2013;11(1):141. doi: 10.1186/1479-5876-11-141. [PubMed][Free full text] Türeci E, İş M, Üzüm G, Akyüz F, Ulu MO, Döşoğlu M, et al. Alterations in blood-brain barrier after traumatic brain injury in streptozotocin-induced diabetic rats. J Nervous Sys Surgery 2009;2(2):79. [Free full text] Van Ye TM, Roza AM, Pieper GM, Henderson J Jr, Johnson JP, Adams MB. Inhibition of intestinal lipid peroxidation does not minimize morphological damage. J Surg Res 1993;55:553. [PubMed] Durak I, Canbolat O, Kavutcu M, Öztürk HS, Yurtarslanı Z. Activities of total, cytoplasmic and mihochondrial superoxide dismutase enzymes in sera and pleural fluids from patient with lung cancer. J Clin Lab Anal 1996;10:17. [PubMed] Aebi H. Catalase. In: H.U.Bergmeyer (Ed): Methods of Enzymatic Analysis, Academic Press , New York and London, 1974;pp.673-677. Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 1974;249:7130. [PubMed] [Free full text] Abdel-Wahhab MA, Nada SA, Arbid MS. Ochratoxicosis: Prevention of developmental toxicity by L-methionine in rats. J Appl Toxicol 1999;19:7. [PubMed] Wolff SP. Diabetes mellitus and free radicals: free radicals, transition metals and oxidative stress in the aetiology of diabetes mellitus and complications. Br Med Bull. 1993;49:642. [PubMed] [Free full text] West IC. Radicals and oxidative stress in diabetes. Diabet Med. 2000;17:171–180. [PubMed] Wanless IR, Lentz JS. Fatty liver hepatitis (steatohepatitis) and obesity: an autopsy study with analysis risk factors. Hepatology. 1990;12:1106. [PubMed] Hotta M, Tashiro F, Ikegami H, Niwa H, Ogihara T, Yodoi J, Miyazaki J. Pancreatic cell-specific expression of thioredoxin, an antioxidative and antiapoptotic protein, prevents autoimmune and streptozotocin-induced diabetes. J Exp Med. 1998;188:1445. [PubMed] [Free full text] Baynes JW. Role of oxidative stress in the development of complications in diabetes. Diabetes. 1991;40:405. [PubMed] Borcea V, Nourooz-Zadeh J, Wolff SP, Klevesath M, Hofmann M, Urich H, et al. α-Lipoic acid decreases oxidative stress even in diabetic patients with poor glycemic control and albuminuria. Free Radic Biol Med. 1999;26:1495. [PubMed] Fitzl G, Martin R, Dettmer D, Hermsdorf V, Drews H, Welt K. Protective effect of ginkgo biloba extract EGb 761 on myocardium of experimentally diabetic rats, I: ultrastructural and biochemical investigation on cardiomyocytes. Exp Toxicol Pathol. 1999;51:189. [PubMed] Bostankolu E, Ayoglu H, Yurtlu S, Okyay RD, Erdogan G, Deniz Y, et al. Dexmedetomidine did not reduce the effects of tourniquet-induced ischemia-reperfusion injury during general anesthesia. Kaohsiung J Med Sci. 2013;29(2):75. doi: 10.1016/j.kjms.2012.08.013. [PubMed] [Free full text] Wakai A, Wang JH, Winter DC, Street JT, O’Sullivan RG, Redmond HP. Tourniquet-induced systemic inflammatory response in extremity surgery. J Trauma 2001;51:922. [PubMed] Concannon MJ, Kester CG, Welsh CF, Puckett CL. Patterns of free-radical production after tourniquet ischemia implications for the hand surgeon. Plast Reconstr Surg 1992;89:846. [PubMed] Grisham MB, Granger DN. Free radicals: reactive metabolites of oxygen as mediators of postischemic reperfusion injury. In: Martson A, Bulkley GB, Fiddian-Green RG, Haglund U, editors. Splanchnic İschemia and Multiple Organ Failure. St Louis, MO: Mosby;1989. pp. 135–144. Mccard JM. The evolution of free radicals and oxidative stress. Am J Med 2000;108:652. [PubMed]

The purpose of this study is to define and describe the semantic components of the verbalised concept “lightness” as a component of ludic competence in the linguistic consciousness of the Russian-speaking people from Eastern Ukraine. The main method of the research was a psycholinguistic experiment. The sample comprised 426 young people (aged 18-35), males and females being equally represented. Cluster analysis showed that the core of the concept “lightness” is represented by three semantic groups: “the quality being light and insignificant in weight and size …”, “the feeling of happiness and joyful ease”, “the feeling of freedom …, cheerfulness, excitement”. The last two clusters reveal the ambivalent nature of the concept “lightness”. The concept “lightness” is characterized by a large variety of peripheral clusters. The ones that are especially noteworthy are “insight” and “duality”. The former reflects the cognitive component of lightness, which accounts for 3 per cent. The latter reflects the concept’s ambivalent nature. Basically, the semantic content of the core of the word “lightness” does not depend on gender. The comparative analysis of the concept “lightness” in the linguistic consciousness of Ukrainian citizens and people living in Russia reveals its nationally-specific perception in the linguistic consciousness of Ukrainian people, which was reflected in the most frequent reaction “freedom”. Taken together, both samples share a number of common features: wide semantic scope; strong synonymic and weak antonymic connections between stimulus and reactions; positive emotional response to the stimulus. Finally, the results of the free word association test with the stimulus word “lightness” were successfully used to define more precisely and expand our understanding of “lightness” as a component of ludic competence taking into account both core and peripheral clusters. References Barnett, L. (2007). The nature of playfulness in young adults. Personality and Individual Differences, 43, 949-958. https://doi.org/10.1016/j.paid.2007.02.018 Bowman, J. (1987). Making Work Play. In G. A. Fine (Ed.), Meaningful Play, Playful Meanings (pp. 61-71). Champaign, IL: Human Kinetics. Bundy, A. (1996). Play and Playfulness: What to Look for. In D.L. Parham & L. S. Fazio (Eds.), Play in Occupational Therapy for Children (pp. 52−66). St. Louis, MO: Mosby. Chapman, J. (1978). Playfulness and the development of divergent thinking abilities. Child: Care, Health and Development, 4, 371-383. https://doi.org/10.1111/j.1365-2214.1978.tb00096.x Csikszentmihalyi, M. (1975). Play and intrinsic rewards. Journal of Humanistic Psychology, 15, 41-63. https://doi.org/10.1111/j.1365-2214.1978.tb00096.x Dal, V. I. (2011). Tolkovyi Slovar Zhivogo Velikorusskogo Yazyka [Explanatory Dictionary of the Living Great Russian Language]: in four volumes. Moscow: Publishing house: Drofa. Glynn, M., & Webster, J. (1992). The adult playfulness scale: an initial assessment. Psychological Reports, 71(1), 83-103. https://doi.org/10.2466/pr0.1992.71.1.83 Gordienko-Mytrofanova, I., & Kobzieva, Iu. (2017). Playful competence: the access code to the inner resources. Proceedings of the 15th European Congress of Psychology Amsterdam, 11-14 July (19). Gordienko-Mytrofanova, I., Pidchasov, Ye., Sauta, S., & Kobzieva, Iu. (2018). The problem of sample representativeness for conducting experimental and broad psychological research. Psycholinguistics, 23(1), 11-46. doi: 10.5281/zenodo.1212360. Groos, K. (1976). The Play of Man: Teasing and Love-Play. In J. Brunner, A. Jolly, & K. Sylva (Eds.), Play, Development and Evolution (pp. 62–83). Middlesex, United Kingdom: Penguin Books. Guitard, P., Ferland, F. & Dutil, É. (2005). Toward a better understanding of playfulness in adults. OTJR: Occupation, Participation and Health, 25(1), 9-22. https://doi.org/10.1177/153944920502500103. Кобзева, Ю., Гордиенко-Митрофанова, И., Гончаренко-Кулиш, А. (2020a). Определение шаловливости как компонента игровой компетентности через реконструкцию семантических элементов концепта «шаловливость». Проблеми сучасної психології, 47, 118-140. Kobzieva Iu., Gordienko-Mytrofanova I., Sauta S. (2020b). Psycholinguistic Features of Imagination as a Component of Ludic Competence. EUREKA: Social and Humanities. Psychology, 2, 15-23. http://dx.doi.org/10.21303/2504-5571.2020.001128 Kobzieva Iu., Gordienko-Mytrofanova I., Udovenko M., Sauta S. (2020c). Concept “humour” in the linguistic consciousness of the Russian-speaking population of Ukraine. European Journal of Humour Research, 8(1), 29-44. http://dx.doi.org/10.7592/EJHR2020.8.1.kobzieva Караулов Ю. Н., Черкасова Г. А., Уфимцева Н. В., Сорокин Ю. А., Тарасов Е. Ф. Русский ассоциативный словарь. В 2-х т. Т. I. От стимула к реакции: ок.7000 стимулов. М.: ООО «Издательство Астрель»: ООО Издательство АСТ». Караулов Ю. Н., Черкасова Г. А., Уфимцева Н. В., Сорокин Ю. А., Тарасов Е. Ф. Русский ассоциативный словарь. В 2-х т. Т. II. От реакции к стимулу: более 100 000 реакций. М.: ООО «Издательство Астрель»: ООО Издательство АСТ». Ожегов, С. И., Шведова, Н. Ю. (2011). Толковый словарь русского языка. Москва: Мир и образование, Оникс. Попова, З. Д., Стернин, И. А. (2007). Семантико-когнитивный анализ языка. Воронеж: Истоки. Proyer, R. (2012). Development and initial assessment of a short measure for adult playfulness: The SMAP. Personality and Individual Differences, 53(8), 989-994. https://doi.org/10.1016/j.paid.2012.07.018 Proyer, R. (2017). A new structural model for the study of adult playfulness: Assessment and exploration of an understudied individual differences variable. Personality and Individual Differences, 108, 113-122. https://doi.org/10.1016/j.paid.2016.12.011 Raven, J. (2001). The Conceptualisation of Competence. New York: Peter Lang Publishing,Inc. Schaefer, C. & Greenberg, R. (1997). Measurement of playfulness: a neglected therapist variable. International Journal of Play Therapy, 6(2), 21-31. https://doi.org/10.1037/h0089406 Shen, X. (2010). Adult Playfulness as a Personality Trait: Its Conceptualization, Measurement, and Relationship to Psychological Well-Being. (Doctoral dissertation). Retrieved from Pennsylvania State University Library Catalog (OCLC No. 859524715). Shen, X., Chick, G. & Zinn, H. (2014). Playfulness in adulthood as a personality trait: a reconceptualization and a new measurement. Journal of Leisure Research, 46(1), 58-83. https://doi.org/10.1080/00222216.2014.11950313 Стернин, И. А., Рудакова, А. В. (2011). Психолингвистическое значение слова и его описание. Воронеж: Ламберт. Tsuji, Hit., Tsuji, Hei., Yamada, S., Natsuno, Y., Morita, Y., Mukoyama, Y., Hata, K. & Fujishima, Y. (1996). Standardization of the Five Factor Personality Questionnaire: Factor structure. International Journal of Psychology, 31. Proceedings from the XXVI International Congress of Psychology. Montreal, 16-21August. (103-217). Уфимцева, Н. (2009). Образ мира русских: системность и содержание. Язык и культура, 98-111. Ушаков, Д. Н. (1935-1940). Толковый словарь русского языка: в четырех томах. Москва: Сов.энциклопедия: ОГИЗ. Yarnal, C. & Qian, X. (2011). Older-adult playfulness: an innovative construct and measurement for healthy aging research. American Journal of Play, 4(1), 52-79. https://files.eric.ed.gov/fulltext/EJ985548.pdf Ефремова, Т. Ф. (2000). Новый словарь русского языка. Толково-словообразовательный. Москва: Русский язык. Епишкин, Н. И. (2010). Историчесикй словарь галлицизмов русского языка. Москва: Словарное издательство ЭТС. Yue, X., Leung, C. & Hiranandani, N. (2016). Adult playfulness, humor styles, and subjective happiness. Psychological Reports, 119(3), 630-640. https://doi.org/10.1177/0033294116662842. Засекина, Л. В. (2008). Тенденції розвитку вітчизняної психолінгвістики: методологічний огляд проблем та окреслення шляхів їх вирішення. Психолінгвістика, 1. С. 9-20. Retrieved from: http://nbuv.gov.ua/UJRN/psling_2008_1_2. References (translated and transliterated) Barnett, L. (2007). The nature of playfulness in young adults. Personality and Individual Differences, 43, 949-958. https://doi.org/10.1016/j.paid.2007.02.018 Bowman, J. (1987). Making Work Play. In G. A. Fine (Ed.), Meaningful Play, Playful Meanings (pp. 61-71). Champaign, IL: Human Kinetics. Bundy, A. (1996). Play and Playfulness: What to Look for. In D.L. Parham & L. S. Fazio (Eds.), Play in Occupational Therapy for Children (pp. 52−66). St. Louis, MO: Mosby. Chapman, J. (1978). Playfulness and the development of divergent thinking abilities. Child: Care, Health and Development, 4, 371-383. https://doi.org/10.1111/j.1365-2214.1978.tb00096.x Csikszentmihalyi, M. (1975). Play and intrinsic rewards. Journal of Humanistic Psychology, 15, 41-63. https://doi.org/10.1111/j.1365-2214.1978.tb00096.x Dal, V. I. (2011). Tolkovyi Slovar Zhivogo Velikorusskogo Yazyka [Explanatory Dictionary of the Living Great Russian Language]: in four volumes. Moscow: Publishing house: Drofa. Glynn, M., & Webster, J. (1992). The adult playfulness scale: an initial assessment. Psychological Reports, 71(1), 83-103. https://doi.org/10.2466/pr0.1992.71.1.83 Gordienko-Mytrofanova, I., & Kobzieva, Iu. (2017). Playful competence: the access code to the inner resources. Proceedings of the 15th European Congress of Psychology Amsterdam, 11-14 July (19). Gordienko-Mytrofanova, I., Pidchasov, Ye., Sauta, S., & Kobzieva, Iu. (2018). The problem of sample representativeness for conducting experimental and broad psychological research. Psycholinguistics, 23(1), 11-46. doi: 10.5281/zenodo.1212360. Groos, K. (1976). The Play of Man: Teasing and Love-Play. In J. Brunner, A. Jolly, & K. Sylva (Eds.), Play, Development and Evolution (pp. 62–83). Middlesex, United Kingdom: Penguin Books. Guitard, P., Ferland, F. & Dutil, É. (2005). Toward a better understanding of playfulness in adults. OTJR: Occupation, Participation and Health, 25(1), 9-22. https://doi.org/10.1177/153944920502500103. Кобзева, Ю., Гордиенко-Митрофанова, И., Гончаренко-Кулиш, А. (2020a). Определение шаловливости как компонента игровой компетентности через реконструкцию семантических элементов концепта «шаловливость». Проблеми сучасної психології, 47, 118-140. Kobzieva, Iu., Gordienko-Mytrofanova, I., Goncharenko-Kulish, A. (2020a). Opredeleniie shalovlivosti kak komponenta igrovoi kompetentosti cherez rekonstruktsiiu semanticheskikh elementov kontsepta “shalovlivost” [Defining impishness as a component of ludic competence via restructuring semantic elements of the concept “impishness”]. Problemy Suchasnoi Psykholohii − Problems of Modern Psychology, 47, 118-140. https://doi.org/10.32626/2227-6246.2020-47 Kobzieva Iu., Gordienko-Mytrofanova I., Sauta S. (2020b). Psycholinguistic Features of Imagination as a Component of Ludic Competence. EUREKA: Social and Humanities. Psychology, 2, 15-23. http://dx.doi.org/10.21303/2504-5571.2020.001128 Kobzieva Iu., Gordienko-Mytrofanova I., Udovenko M., Sauta S. (2020c). Concept “humour” in the linguistic consciousness of the Russian-speaking population of Ukraine. European Journal of Humour Research, 8(1), 29-44. http://dx.doi.org/10.7592/EJHR2020.8.1.kobzieva Karaulov, Yu. N., Cherkasova, G. A., Ufimtseva, N. V., Sorokin, Yu. A., & Tarasov, Ye. F. (2002a). Russkii Assotsiativnyi Slovar [Russian Associative Vocabulary], Vol. 1. Ot reaktsii k stimulu [From Reaction to Stimulus], ca. 100000 reactions. Мoscow: LLC Astrel Publishers; LLC AST Publishers. Karaulov, Yu. N., Cherkasova, G. A., Ufimtseva, N. V., Sorokin, Yu. A., & Tarasov, Ye. F. (2002b). Russkii Assotsiativnyi Slovar [Russian Associative Vocabulary], Vol. 2. Ot stimula k reaktsii [From Stimulus to Reaction], ca. 7000 stimuli. Мoscow: LLC Astrel Publishers; LLC AST Publishers. Ожегов, С. И., Шведова, Н. Ю. (2011). Толковый словарь русского языка. Москва: Мир и образование, Оникс. Ozhegov, S. I. & Shvedova, N. Yu. (2011). Tolkovyi Slovar Russkogo Yazyka [Dictionary of Russian Language]. Мoscow: Mir i Obrazovaniie, Oniks. Попова, З. Д., Стернин, И. А. (2007). Семантико-когнитивный анализ языка. Воронеж: Истоки. Popova, Z. D. & Sternin, I. A. (2007). Semantiko-Kognitivnyi Analiz Yazyka [Semantic and Cognitive Analysis of Language]. Voronezh: Istoki. Proyer, R. (2012). Development and initial assessment of a short measure for adult playfulness: The SMAP. Personality and Individual Differences, 53(8), 989-994. https://doi.org/10.1016/j.paid.2012.07.018 Proyer, R. (2017). A new structural model for the study of adult playfulness: Assessment and exploration of an understudied individual differences variable. Personality and Individual Differences, 108, 113-122. https://doi.org/10.1016/j.paid.2016.12.011 Raven, J. (2001). The Conceptualisation of Competence. New York: Peter Lang Publishing,Inc. Schaefer, C. & Greenberg, R. (1997). Measurement of playfulness: a neglected therapist variable. International Journal of Play Therapy, 6(2), 21-31. https://doi.org/10.1037/h0089406 Shen, X. (2010). Adult Playfulness as a Personality Trait: Its Conceptualization, Measurement, and Relationship to Psychological Well-Being. (Doctoral dissertation). Retrieved from Pennsylvania State University Library Catalog (OCLC No. 859524715). Shen, X., Chick, G. & Zinn, H. (2014). Playfulness in adulthood as a personality trait: a reconceptualization and a new measurement. Journal of Leisure Research, 46(1), 58-83. https://doi.org/10.1080/00222216.2014.11950313 Sternin, I. A., & Rudakova, A. V. (2011). Psikholingvisticheskoie znacheniie slova i yego opisaniie [Psycholinguistic meaning of the word and its description]. Voronezh: Lambert Tsuji, Hit., Tsuji, Hei., Yamada, S., Natsuno, Y., Morita, Y., Mukoyama, Y., Hata, K. & Fujishima, Y. (1996). Standardization of the Five Factor Personality Questionnaire: Factor structure. International Journal of Psychology, 31. Proceedings from the XXVI International Congress of Psychology. Montreal, 16-21August. (103-217). Ufimtseva, N. (2009). Obraz mira russkikh: sistemnost i soderzhaniie [Image of the world of Russians: the systemic characteristics and the content]. Yazyk i Kultura − Language and Culture, 98-111. Ushakov, D. N. (Ed.). (1935-1940). Tolkovyi Slovar Russkogo Yazyka [Dictionary of Russian Language]: in four volumes. Moscow: Sov. Encyclopedia: OGIZ. http://feb-web.ru/feb/ushakov/ush-abc/0ush.htm Yarnal, C. & Qian, X. (2011). Older-adult playfulness: an innovative construct and measurement for healthy aging research. American Journal of Play, 4(1), 52-79. https://files.eric.ed.gov/fulltext/EJ985548.pdf Yefremova, T. F. (2000). Novyi Slovar Russkogo Yazyka. Tolkovo-Slovoobrazovatelnyi [New Dictionary of the Russian Language. Interpretative and Derivational]. Moscow: Russkii yazyk. https://www.efremova.info/ Yepishkin, N. I. (2010). Istoricheskii slovar gallitsizmov russkogo yazyka [Historical Dictionary of Gallicisms in the Russian Language]. Moscow: ETS Dictionary Publishing House. Retrieved from: http://rus-yaz.niv.ru/doc/gallism-dictionary/index.htm Yue, X., Leung, C. & Hiranandani, N. (2016). Adult playfulness, humor styles, and subjective happiness. Psychological Reports, 119(3), 630-640. https://doi.org/10.1177/0033294116662842. Zasiekina, L. V. (2008). Tendentsiii rozvytku vitchyznianoii psykholingvistyky: metodolohichnyi ohliad problem ta okreslennia shlyakhiv yikh vyrishennia [Trends in the development of national psycholinguistics: a methodological overview of problems and outlining ways to solve them]. Psycholinguistics, 1, 9-20. Retrieved from: http://nbuv.gov.ua/UJRN/psling_2008_1_2.

News and Announcements1) Canadian Children's Book News, Spring 2015 IssueIn recognition of the TD Canadian Children's Book Week and its theme "Hear Our Stories: Celebrating First Nations, Métis and Inuit Literature," this issue explores several facets of this vibrant part of children's literature. It includes a profile of author David Alexander Robertson and a look at the publishers and market for First Nations, Métis, and Inuit stories.2) TD Canadian Children's Book Week (May 2- May 9, 2015) is the single most important national event celebrating Canadian children’s books and the importance of reading. More than 28,000 children, teens, and adults participate in activities held in every province and territory across the country. Hundreds of schools, public libraries, bookstores, and community centres host events as part of this major literary festival. It is organized by the Canadian Children’s Book Centre, in partnership with the Storytellers of Canada/Conteurs du Canada.3) Free Comic Book Day (May 2, 2015) takes place annually on the first Saturday in May. It is a single day when participating comic book specialty shops and public libraries across North America and around the world give away comic books absolutely free to anyone who comes into their shops! For more information: http://www.freecomicbookday.com/Home/1/1/27/9924) Canadian Authors for Indies Day (May 2, 2015)Authors across Canada support independent bookstores by volunteering as guest book sellers. To see who may be in your local indie book store, go to http://www.authorsforindies.com/5) Storytellers of Canada/Conteurs du Canada conference: Where Languages Meet (July 2-5, 2015). This year’s conference is in Lévis, Quebec where a rich storytelling tradition awaits. La Maison Natale Louis Fréchette – birthplace of one of Quebec’s most celebrated poets – hosts the SC-CC conference which proudly brings a range of vibrant programming in both official languages storytellers and listeners. http://www.storytellers-conteurs.ca/en/conference/storytellers-conference-2015.html6) Words in 3 Dimensions Conference 2015: Intersections (May 22 to 24, 2015)Held at the Chateau Lacombe Hotel in Edmonton for this second edition, the conference connects writers, editors, publishers, and agents from across Canada. This weekend focuses on how and where a writer’s work with words intersects with other disciplines. http://www.wordsin3d.com/7) The 2015 Storytelling World Resource Awards (storytellingworld.com/2015/) includes the following Canadian titles :Stories for Pre-Adolescent Listeners: Not My Girl: the True Sotry of a Daughter's Cultural Adjustmentsby Christy Jordan-Fenton and Margaret Pokiak-Fenton (Annick Press)Stories for Adolescent Listeners: Hope Springs: a Story of Complassion and understanding by Eric Walters (Tundra Books)8) IBBY Canada (International Board on Books for Young People, Canadian section). Stop, Thief!, illustrated by Pierre Pratt and written by Heather Tekavec (Kids Can Press, 2014), is the winner of the Elizabeth Mrazik-Cleaver Canadian Picture Book Award. Pierre was also nominated [again] by IBBY Canada for the prestigious Hans Christian Andersen Award.” www.ibby-canada.org/elizabeth-mrazik-cleaver-pratt/And now, a plethora of shortlist announcements:1) The 2015 Alberta Literary Awards ShortlistWinners will be announced and awards presented at the Alberta Literary Awards Gala on Saturday, May 23, 2015. The celebration will take place at the Chateau Lacombe Hotel (10111 Bellamy Hill Road) in Edmonton alongside the 2015 Words in 3 Dimensions Conference: Intersections (see above).A full list of award categories and nominees can be found at http://writersguild.ca/2015-alberta-literary-awards-shortlist/2) R. Ross Annett Award for Children's Literature (www.bookcentre.ca/awards/r_ross_annett_award_childrens_literature) Victor Lethbridge– You're Just Right (Tatanka Books)Leanne Shirtliffe– The Change Your Name Store (Sky Pony Press)Richard Van Camp– Little You (Orca Book Publishers) 3) 2014 Science in Society Book Awards Shortlists. Two annual book awards honour outstanding contributions to science writing. One is for books intended for children ages 8-12; the other for book aimed at the general public. Winners will be announced on Canada Book Day, April 23, 2015. http://sciencewriters.ca/awards/book-awards/Zoobots by Helaine Becker, Kids Can Press.Starting from Scratch by Sarah Elton, Owl Kids Books.It’s Catching by Jennifer Gardy, Owl Kids Books.The Fly by Elise Gravel, Penguin Random House.If by David J. Smith, Kids Can Press.4) 2015 Atlantic Book Awards ShortlistThe full shortlist for the eight different book prizes comprising the 2015 Atlantic Book Awards can be found www.atlanticbookawards.ca. Below are the nominees for the Ann Connor Brimer Award for Children’s Literature and the Lillian Shepherd Award for Excellence in Illustration. Winners will be announced Thursday, May 14, 2015.Ann Connor Brimer Award for Children’s LiteratureJack, the King of Ashes by Andy Jones (Running Goat Books & Broadsides)Flame and Ashes: The Great Fire Diary of Triffie Winsor (Dear Canada series) by Janet McNaughton (Scholastic Canada Ltd.)The End of the Line by Sharon E. McKay (Annick Press Ltd.)Lillian Sheperd Award for Excellence in IllustrationSydney Smith (nominee) Music is for Everyone by Jill Barber (Nimbus Publishing)Michael Pittman (nominee) Wow Wow and Haw Haw by George Murray(Breakwater Books)Nancy Rose (nominee) The Secret Life of Squirrels by Nancy Rose (Penguin Canada)5) Newfoundland and Labrador Book Award shortlist.During even-numbered years, these awards honour fiction and children’s/young adult fiction books; odd-numbered years recognise poetry and non-fiction. The winners will be announced May 27, 2015. This year’s list of finalists for the Newfoundland and Labrador Non-fiction Award are all first-time authors (http://wanl.ca/literary_awards)Alan Doyle for Where I Belong: From Small Town to Great Big Sea (Doubleday Canada)Janet Merlo for No One to Tell: Breaking My Silence on Life in the RCMP (Breakwater Books)Andrew Peacock for Creatures of the Rock (Doubleday Canada)Three acclaimed Newfoundland poets are shortlisted for the E.J. Pratt Poetry Award:Michael Crummey for Under the Keel(House of Anansi Press)Mary Dalton for Hooking (Véhicule Press)Carmelita McGrath for Escape Velocity (Goose Lane Editions)6) 2015 Information Book Award Shortlist announced by the Children’s Literature Roundtables of Canada. Voting Deadline: Saturday October 31, 2015.Any Questions? by Marie-Louise Gay.(Groundwood Books). A Brush Full of Colour: The World of Ted Harrison. by Margriet Ruurs & Katherine Gibson (Pajama Press).Do You Know Komodo Dragons? by Alain M. Bergeron, Michel Quintin, and Sampar. Illustrations by Sampar. Translated by Solange Messier (Fitzhenry & Whiteside).Dreaming in Indian: Contemporary Native American Voices. edited by Lisa Charleyboy and Mary Beth Leatherdale (Annick Press). Not My Girl. by Christy Jordan-Fenton and Margaret Pokiak-Fenton. Illustrated by Gabrielle Grimard (Annick Press). The Rat. by Elise Gravel (Tundra Books). Shapes in Math, Science and Nature: Squares, Triangles and Circles. by Catherine Sheldrick Ross. Illustrated by Bill Slavin (Kids Can Press). Take Shelter: At Home Around the World. by Nikki Tate and Dani Tate-Stratton (Orca Books). Tastes Like Music: 17 Quirks of the Brain and Body. by Maria Birmingham. Illustrated by Monika Melnychuk (Owl Kids). We All Count: A Book of Cree Numbers. by Julie Flett (Native Northwest).For more information about voting and submissions please contact the Information Book Award Chair, Kay Weisman at weismankay@gmail.com7) IBBY Canada (International Board on Books for Young People, Canadian section).Stop, Thief! illustrated by Pierre Pratt and written by Heather Tedavec (Kids Can Press, 2014) is the winner of the Elizabeth Mrazik-Cleaver Canadian Picture Book Award. Pierre was also nominated [again] by IBBY Canada for the prestigious ans Chrisitan Andersen Award. (www.ibby-canada.org/elizabeth-mrazik-cleaver-pratt/)-----Presented by Gail de Vos. Gail is an adjunct professor who teaches courses on Canadian children's literature, young adult literature, and commic books and graphic novels at the School of Library and Information Studies (SLIS) at the University of Alberta and is the author of nine books on storytelling and folklore. She is a professional storyteller and has taught the storytelling course at SLIS for over two decades.

The article is focused on the study of the perception of Lesya Ukrainka, a famous Ukrainian writer, in contemporary Ukrainian society. The research is based on a free word association test held online with 200 respondents aged from 13 to 70. As a result of applying quantitative analysis of the associates and semantic gestalt method the authors singled out productive semantic zones concerning each of the stimuli. These zones presented an anthroponymic triad of personality identification related to the author’s names ‘Larysa Kvitka’, ‘Larysa Kosach’, and the pseudonym ‘Lesya Ukrainka’. The nuclear zones in each associative field manifest a tendency for uniformity. They are related to her professional activities, her works, elements of inner and outer portrayal, as well as of evaluative spectrum. The respondents have shown predominantly high levels of knowledge about Lesya Ukrainka’s personality, which is proven, in particular, by their reverse frequency reactions and peripheral character of zero reactions. A dominant positive evaluative spectrum of perception of Lesya Ukrainka, as well as productivity of individual associates of interpretational character, was also important. References Barnett, L. (2007). The nature of playfulness in young adults. Personality and Individual Differences, 43, 949-958. Bowman, J. (1987). Making Work Play. In G. A. Fine (Ed.), Meaningful Play, Playful Meanings (pp. 61-71). Champaign, IL: Human Kinetics. Bundy, A. (1996). Play and Playfulness: What to Look for. In D.L. Parham & L. S. Fazio (Eds.), Play in Occupational Therapy for Children (pp. 52−66). St. Louis, MO: Mosby. Chapman, J. (1978). Playfulness and the development of divergent thinking abilities. Child: Care, Health and Development, 4, 371-383. Csikszentmihalyi, M. (1975). Play and intrinsic rewards. Journal of Humanistic Psychology, 15, 41-63. Dal, V. I. (2011). Explanatory Dictionary of the Living Great Russian Language: in four volumes. Publishing house: Drofa. Retrieved from: http://slovardalja.net/ Glynn, M., & Webster, J. (1992). The adult playfulness scale: an initial assessment. Psychological Reports, 71 (1), 83-103. https://doi.org/10.2466/pr0.1992.71.1.83 Гордиенко-Митрофанова, И.В. (2014а). Лексикографическое значение слова «игривость» (подготовительный этап психолингвистического эксперимента). Психологічні перспективи, 24, 76-88. Гордиенко-Митрофанова, И.В. (2014b). Психологическое содержание лексикографических значений слова «игривый» (подготовительный этап психолингвистического эксперимента). Проблеми сучасної педагогічної освіти, 46 (3), 298-306. Gordienko-Mytrofanova, I., & Kobzieva, Iu. (2017a). Playful competence: the access code to the inner resources. Proceedings of the 15th European Congress of Psychology. Amsterdam, 11-14 July. (19) Gordienko-Mytrofanova, I., & Kobzieva, Iu. (2017b). Humour as a component of ludic competence. Visnyk of H.S. Skovoroda Kharkiv National Pedagogical University, Psychology, 57, 40-56. Gordienko-Mytrofanova, I. & Kobzieva, Iu. (2018). Concept “holy fool” in the linguistic world-image of the Russian-speaking population of Ukraine. Psycholinguistics-Psiholingvistika, 24(1), 118-133. https://doi.org/10.31470/2309-1797-2018-24-1-118-133 Gordienko-Mytrofanova, I. & Kobzieva, Iu. (2019). Gender- and role-specific differences in the perception of the concept “impishness” (based on the results of a psycholinguistic experiment). Psycholinguistics-Psiholingvistika, 25(1), 33-48. https://doi.org/10.31470/2309-1797-2019-25-1-33-48 Gordienko-Mytrofanova, I., Kobzieva, Iu. & Silina, A. (2018a). Psycholinguistic meanings of the verbalised concept “holy fool” (based on the results of the psycholinguistic experiment). Vіsnyk of H. S. Skovoroda Kharkiv National Pedagogical University, Psychology, 59, 18-34. https://doi.org/10.5281/zenodo.2527863 Gordiienko-Mytrofanova, I., Kobzieva, I. & Sauta, S. (2019). Psycholinguistic meanings of playfulness. East European Journal of Psycholinguistics, 6(1), 19-31. https://doi.org/10.5281/zenodo.3371627 Gordienko-Mytrofanova, I., Pidchasov, Ye., Sauta, S., & Kobzieva, Iu. (2018b). The problem of sample representativeness for conducting experimental and broad psychological research. Psycholinguistics-Psiholingvistika, 23(1), 11-46. https://doi.org/10.5281/zenodo.1212360 Gordiienko-Mytrofanova, I. V., & Sauta, S. L. (2016). Playfulness as a peculiar expression of sexual relationships (semantic interpretation of the results of the psycholinguistic experiment). European Humanities Studies: State and Society, 1, 46-62. Retrieved from: http://ehs-ss.pl/czasopismo/EHS-SS-01-2016.pdf Gordiienko-Mytrofanova, I. & Sypko, A. (2015). Playfulness as a relevant lexeme in the bilingual linguistic consciousness of Ukrainian people. East European Journal of Psycholinguistics, 2(1), 43-51. Retrieved from http://esnuir.eenu.edu.ua/bitstream/ 123456789/9355/1/eejpl_journal_2_1_2015_sypko_hordiyenko_mytrofanova.pdf Groos, K. (1976). The Play of Man: Teasing and Love-Play. In J. Brunner, A. Jolly, & K. Sylva (Eds.), Play, Development and Evolution (pp. 62–83). Middlesex, United Kingdom: Penguin Books. Guitard, P., Ferland, F. & Dutil, É. (2005). Toward a better understanding of playfulness in adults. OTJR: Occupation, Participation and Health, 25 (1), 9-22. https://doi.org/10.1177/153944920502500103 Кобзева, Ю., Гордиенко-Митрофанова, И., Гончаренко-Кулиш, А. (2020a). Определение шаловливости как компонента игровой компетентности через реконструкцию семантических элементов концепта «шаловливость». Проблеми сучасної психології, 47, 118-140. https://doi.org/10.32626/2227-6246.2020-47 Kobzieva Iu., Gordienko-Mytrofanova I., Sauta S. (2020b). Psycholinguistic Features of Imagination as a Component of Ludic Competence. EUREKA: Social and Humanities, 2, 15-23. http://dx.doi.org/10.21303/2504-5571.2020.001128 Kobzieva Iu., Gordienko-Mytrofanova I., Udovenko M., Sauta S. (2020c). Concept “humour” in the linguistic consciousness of the Russian-speaking population of Ukraine. European Journal of Humour Research, 8(1), 29-44. http://dx.doi.org/10.7592/EJHR2020.8.1.kobzieva. Караулов Ю. Н., Черкасова Г. А., Уфимцева Н. В., Сорокин Ю. А., Тарасов Е. Ф. Русский ассоциативный словарь. В 2-х т. Т. I. От стимула к реакции: ок.7000 стимулов. М.: ООО «Издательство Астрель»: ООО Издательство АСТ». Караулов Ю. Н., Черкасова Г. А., Уфимцева Н. В., Сорокин Ю. А., Тарасов Е. Ф. Русский ассоциативный словарь. В 2-х т. Т. II. От реакции к стимулу: более 100 000 реакций. М.: ООО «Издательство Астрель»: ООО Издательство АСТ». Ожегов, С. И., Шведова, Н. Ю. (2011). Толковый словарь русского языка. Москва: Мир и образование, Оникс. Попова, З. Д., Стернин, И. А. (2007). Семантико-когнитивный анализ языка. Воронеж: Истоки. Proyer, R. (2012). Development and initial assessment of a short measure for adult playfulness: The SMAP. Personality and Individual Differences, 53 (8), 989-994. https://doi.org/10.1016/j.paid.2012.07.018 Proyer, R. (2017). A new structural model for the study of adult playfulness: Assessment and exploration of an understudied individual differences variable. Personality and Individual Differences, 108, 113-122. https://doi.org/10.1016/j.paid.2016.12.011 Raven, J. (2001). The Conceptualisation of Competence. New York: Peter Lang. Schaefer, C. & Greenberg, R. (1997). Measurement of playfulness: a neglected therapist variable. International Journal of Play Therapy, 6(2), 21-31. https://doi.org/10.1037/h0089406 Shen, X. (2010). Adult Playfulness as a Personality Trait: Its Conceptualization, Measurement, and Relationship to Psychological Well-Being. (Doctoral dissertation). Retrieved from Pennsylvania State University Library Catalog (OCLC No. 859524715). Shen, X., Chick, G. & Zinn, H. (2014). Playfulness in adulthood as a personality trait: a reconceptualization and a new measurement. Journal of Leisure Research, 46(1), 58-83. https://doi.org/10.1080/00222216.2014.11950313 Стернин, И. А., Рудакова, А. В. (2011). Психолингвистическое значение слова и его описание. Воронеж: Ламберт. Tsuji, Hit., Tsuji, Hei., Yamada, S., Natsuno, Y., Morita, Y., Mukoyama, Y., Hata, K. & Fujishima, Y. (1996). Standardization of the Five Factor Personality Questionnaire: Factor structure. International Journal of Psychology, 31. Proceedings from the XXVI International Congress of Psychology Montreal, 16-21August (103-217). Уфимцева, Н. (2009). Образ мира русских: системность и содержание. Язык и культура, 98-111. Ушаков, Д. Н. (1935-1940). Толковый словарь русского языка: в четырех томах. Москва: Сов.энциклопедия: ОГИЗ. Yarnal, C. & Qian, X. (2011). Older-adult playfulness: an innovative construct and measurement for healthy aging research. American Journal of Play, 4 (1), 52-79. https://files.eric.ed.gov/fulltext/EJ985548.pdf Ефремова, Т. Ф. (2000). Новый словарь русского языка. Толково-словообразовательный. Москва: Русский язык. Епишкин, Н. И. (2010). Историчесикй словарь галлицизмов русского языка. Словарное издательство ЭТС. Yue, X., Leung, C. & Hiranandani, N. (2016). Adult playfulness, humor styles, and subjective happiness. Psychological Reports, 119 (3), 630-640. https://doi.org/10.1177/0033294116662842 Засекина, Л. В. (2008). Тенденції розвитку вітчизняної психолінгвістики: методологічний огляд проблем та окреслення шляхів їх вирішення. Retrieved from: Psycholinguistics-Psiholingvistika, 1. Retrieved from: http://nbuv.gov.ua/UJRN/psling_2008_1_2 References (translated and transliterated) Barnett, L. (2007). The nature of playfulness in young adults. Personality and Individual Differences, 43, 949-958. Bowman, J. (1987). Making Work Play. In G. A. Fine (Ed.), Meaningful Play, Playful Meanings (pp. 61-71). Champaign, IL: Human Kinetics. Bundy, A. (1996). Play and Playfulness: What to Look for. In D.L. Parham & L. S. Fazio (Eds.), Play in Occupational Therapy for Children (pp. 52−66). St. Louis, MO: Mosby. Chapman, J. (1978). Playfulness and the development of divergent thinking abilities. Child: Care, Health and Development, 4, 371-383. Csikszentmihalyi, M. (1975). Play and intrinsic rewards. Journal of Humanistic Psychology, 15, 41-63. Dal, V. I. (2011). Tolkovyi Slovar Zhivogo Velikorusskogo Yazyka [Explanatory Dictionary of the Living Great Russian Language]: in four volumes. Publishing house: Drofa. Retrieved from: http://slovardalja.net/ Glynn, M., & Webster, J. (1992). The adult playfulness scale: an initial assessment. Psychological Reports, 71(1), 83-103. https://doi.org/10.2466/pr0.1992.71.1.83 Gordiienko-Mytrofanova, I.V. (2014a). Leksikograficheskoie znacheniie slova “igrivost” (podgotovitelnyi etap psikholingvisticheskogo eksperimenta) [The lexicographic meaning of the word “playfulness” (preparatory stage of a psycholinguistic experiment)]. Psykholohichni Perspektyvy − Psychological Prospects, 24, 76-88. Gordiienko-Mytrofanova, I.V. (2014b). Psikhologicheskoie soderzhaniie leksikograficheskikh znachenii slova “igrivyi” (podgotovitelnyi etap psikholingvisticheskogo eksperimenta) [The psychological content of lexicographic meanings of the word “playful” (preparatory stage of the psycholinguistic experiment)]. Problemy suchasnoi pedahohichnoi osvity − Problems of Modern Pedagogical Education, 46(3), 298-306. Gordiienko-Mytrofanova, I.V. (2014c). Psikhologicheskaia interpretatsiia leksikograficheskogo opisaniia slova “igrivyi” [Psychological interpretation of the lexicographic description of the word “playful”]. Problemy Suchasnoi Psykholohii − Problems of Modern Psychology, 25, 83-98. Gordienko-Mytrofanova, I., & Kobzieva, Iu. (2017a). Playful competence: the access code to the inner resources. Proceedings of the 15th European Congress of Psychology Amsterdam, 11-14 July. (19). Gordienko-Mytrofanova, I., & Kobzieva, Iu. (2017b). Humour as a component of ludic competence. Visnyk of H.S. Skovoroda Kharkiv National Pedagogical University, Psychology, 57, 40-56. Gordienko-Mytrofanova, I. & Kobzieva, Iu. (2018). Concept “holy fool” in the linguistic world-image of the Russian-speaking population of Ukraine. Psycholinguistics- Psiholingvistika, 24 (1), 118-133. https://doi.org/10.31470/2309-1797-2018-24-1-118-133 Gordienko-Mytrofanova, I. & Kobzieva, Iu. (2019). Gender- and role-specific differences in the perception of the concept “impishness” (based on the results of a psycholinguistic experiment). Psycholinguistics-Psiholingvistika, 25(1), 33-48. https://doi.org/10.31470/2309-1797-2019-25-1-33-48 Gordienko-Mytrofanova, I., Kobzieva, Iu. & Silina, A. (2018a). Psycholinguistic meanings of the verbalised concept “holy fool” (based on the results of the psycholinguistic experiment). Vіsnyk of H. S. Skovoroda Kharkiv National Pedagogical University, Psychology, 59, 18-34. https://doi.org/10.5281/zenodo.2527863 Gordiienko-Mytrofanova, I., Kobzieva, I. & Sauta, S. (2019). Psycholinguistic meanings of playfulness. East European Journal of Psycholinguistics, 6(1), 19-31. https://doi.org/10.5281/zenodo.3371627 Gordienko-Mytrofanova, I., Pidchasov, Ye., Sauta, S., & Kobzieva, Iu. (2018b). The problem of sample representativeness for conducting experimental and broad psychological research. Psycholinguistics-Psiholingvistika, 23(1), 11-46. https://doi.org/10.5281/zenodo.1212360 Gordiienko-Mytrofanova, I. V., & Sauta, S. L. (2016). Playfulness as a peculiar expression of sexual relationships (semantic interpretation of the results of the psycholinguistic experiment). European Humanities Studies: State and Society, 1, 46-62. Retrieved from: http://ehs-ss.pl/czasopismo/EHS-SS-01-2016.pdf Gordiienko-Mytrofanova, I. & Sypko, A. (2015). Playfulness as a relevant lexeme in the bilingual linguistic consciousness of Ukrainian people. East European Journal of Psycholinguistics, 2(1), 43-51. Retrieved from: http://esnuir.eenu.edu.ua/bitstream/ 123456789/9355/1/eejpl_journal_2_1_2015_sypko_hordiyenko_mytrofanova.pdf Groos, K. (1976). The Play of Man: Teasing and Love-Play. In J. Brunner, A. Jolly, & K. Sylva (Eds.), Play, Development and Evolution (pp. 62–83). Middlesex, United Kingdom: Penguin Books. Guitard, P., Ferland, F. & Dutil, É. (2005). Toward a better understanding of playfulness in adults. OTJR: Occupation, Participation and Health, 25 (1), 9-22. https://doi.org/10.1177/153944920502500103 Kobzieva, Iu., Gordienko-Mytrofanova, I., Goncharenko-Kulish, A. (2020a). Opredeleniie shalovlivosti kak komponenta igrovoi kompetentosti cherez rekonstruktsiiu semanticheskikh elementov kontsepta “shalovlivost” [Defining impishness as a component of ludic competence via restructuring semantic elements of the concept “impishness”]. Problemy Suchasnoi Psykholohii − Problems of Modern Psychology, 47, 118-140. https://doi.org/10.32626/2227-6246.2020-47 Kobzieva Iu., Gordienko-Mytrofanova I., Sauta S. (2020b). Psycholinguistic Features of Imagination as a Component of Ludic Competence. EUREKA: Social and Humanities, 2, 15-23. http://dx.doi.org/10.21303/2504-5571.2020.001128 Kobzieva Iu., Gordienko-Mytrofanova I., Udovenko M., Sauta S. (2020c). Concept “humour” in the linguistic consciousness of the Russian-speaking population of Ukraine. European Journal of Humour Research, 8(1), 29-44. http://dx.doi.org/10.7592/EJHR2020.8.1.kobzieva Karaulov, Yu. N., Cherkasova, G. A., Ufimtseva, N. V., Sorokin, Yu. A., & Tarasov, Ye. F. (2002a). Russkii Assotsiativnyi Slovar [Russian Associative Vocabulary], Vol. 1. Ot reaktsii k stimulu [From Reaction to Stimulus], ca. 100000 reactions. Мoscow: LLC Astrel Publishers; LLC AST Publishers. Karaulov, Yu. N., Cherkasova, G. A., Ufimtseva, N. V., Sorokin, Yu. A., & Tarasov, Ye. F. (2002b). Russkii Assotsiativnyi Slovar [Russian Associative Vocabulary], Vol. 2. Ot stimula k reaktsii [From Stimulus to Reaction], ca. 7000 stimuli. Мoscow: LLC Astrel Publishers; LLC AST Publishers. Ozhegov, S. I. & Shvedova, N. Yu. (2011). Tolkovyi Slovar Russkogo Yazyka [Dictionary of Russian Language]. Мoscow: Mir i Obrazovaniie, Oniks. Popova, Z. D. & Sternin, I. A. (2007). Semantiko-Kognitivnyi Analiz Yazyka [Semantic and Cognitive Analysis of Language]. Voronezh: Istoki. Proyer, R. (2012). Development and initial assessment of a short measure for adult playfulness: The SMAP. Personality and Individual Differences, 53(8), 989-994. https://doi.org/10.1016/j.paid.2012.07.018 Proyer, R. (2017). A new structural model for the study of adult playfulness: Assessment and exploration of an understudied individual differences variable. Personality and Individual Differences, 108, 113-122. https://doi.org/10.1016/j.paid.2016.12.011 Raven, J. (2001). The Conceptualisation of Competence. New York: Peter Lang. Schaefer, C. & Greenberg, R. (1997). Measurement of playfulness: a neglected therapist variable. International Journal of Play Therapy, 6 (2), 21-31. https://doi.org/10.1037/h0089406 Shen, X. (2010). Adult Playfulness as a Personality Trait: Its Conceptualization, Measurement, and Relationship to Psychological Well-Being. (Doctoral dissertation). Retrieved from Pennsylvania State University Library Catalog (OCLC No. 859524715). Shen, X., Chick, G. & Zinn, H. (2014). Playfulness in adulthood as a personality trait: a reconceptualization and a new measurement. Journal of Leisure Research, 46 (1), 58-83. https://doi.org/10.1080/00222216.2014.11950313 Sternin, I. A., & Rudakova, A. V. (2011). Psikholingvisticheskoie znacheniie slova i yego opisaniie [Psycholinguistic meaning of the word and its description]. Voronezh: Lambert Tsuji, Hit., Tsuji, Hei., Yamada, S., Natsuno, Y., Morita, Y., Mukoyama, Y., Hata, K. & Fujishima, Y. (1996). Standardization of the Five Factor Personality Questionnaire: Factor structure. International Journal of Psychology, 31. Proceedings from the XXVI International Congress of Psychology Montreal, 16-21August (103-217). Ufimtseva, N. (2009). Obraz mira russkikh: sistemnost i soderzhaniie [Image of the world of Russians: the systemic characteristics and the content]. Yazyk i Kultura − Language and Culture, 98-111. Ushakov, D. N. (Ed.). (1935-1940). Tolkovyi Slovar Russkogo Yazyka [Dictionary of Russian Language]: in four volumes. Moscow: Sov. Encyclopedia: OGIZ. http://feb-web.ru/feb/ushakov/ush-abc/0ush.htm Yarnal, C. & Qian, X. (2011). Older-adult playfulness: an innovative construct and measurement for healthy aging research. American Journal of Play, 4(1), 52-79. Retrieved from: https://files.eric.ed.gov/fulltext/EJ985548.pdf Yefremova, T. F. (2000). Novyi Slovar Russkogo Yazyka. Tolkovo-Slovoobrazovatelnyi [New Dictionary of the Russian Language. Interpretative and Derivational]. Moscow: Russkii yazyk. Retrieved from: https://www.efremova.info/ Yepishkin, N. I. (2010). Istoricheskii slovar gallitsizmov russkogo yazyka [Historical Dictionary of Gallicisms in the Russian Language]. ETS Dictionary Publishing House. Retrieved from: http://rus-yaz.niv.ru/doc/gallism-dictionary/index.htm Yue, X., Leung, C. & Hiranandani, N. (2016). Adult playfulness, humor styles, and subjective happiness. Psychological Reports, 119(3), 630-640. https://doi.org/10.1177/0033294116662842 Zasiekina, L. V. (2008). Tendentsiii rozvytku vitchyznianoii psykholingvistyky: metodolohichnyi ohliad problem ta okreslennia shlyakhiv yikh vyrishennia [Trends in the development of national psycholinguistics: a methodological overview of problems and outlining ways to solve them]. Psycholinguistics-Psiholingvistika, 1. Retrieved from: http://nbuv.gov.ua/UJRN/psling_2008_1_2

Abstract Background Vancomycin (VAN) therapy for C. difficile infection (CDI) is effective with &gt; 80% clinical response (CR) but is associated with 20–30% recurrence rate (rCDI). Secondary bile acids (2° BAs) inhibit C. difficile germination and help prevent rCDI. VAN depletes the gut microbiome decreasing the conversion of primary bile acids to 2° BAs. Ridinilazole (RDZ) is a highly selective anti-CDI, DNA-binding antibiotic in development for the treatment of CDI and prevention of rCDI. Methods A global, double-blinded, randomized Phase 3 trial assessed a 10-day treatment with RDZ 200 mg BID vs VAN 125 mg QID for CDI. The primary endpoint was sustained clinical response (SCR) defined as CR and no rCDI through 30 days post-end of treatment (EOT). Other endpoints included rCDI, microbiome diversity and composition, and microbiome-derived 2° BAs concentration. rCDI was defined as a new episode of diarrhea with confirmed positive free toxin test (FTT), requiring additional CDI therapy. All participants were monitored for treatment emergent adverse events (TEAE). Results Of the 759 patients (pts) enrolled, 745 were included in the mITT population (RDZ n=370, VAN n=375). RDZ achieved a numerically higher SCR rate than VAN (73.0% vs 70.7%) p=0.4672. RDZ resulted in a significant reduction in rCDI rate (8.1% vs 17.3%, p=0.0002) (Fig 1). In a pre-specified subpopulation, this was most notable in pts not receiving other antibiotics (rCDI 6.7% in RDZ vs 16.5% in VAN, p=0.0005). Microbiome alpha diversity was higher for RDZ vs VAN at EOT and EOT+30d (p&lt; 0.0001 and p≤ 0.0007 respectively, Fig 2) as were relative abundance (p&lt; 0.0001 and p=0.0203 respectively), and concentrations of 2° BAs (Fig 3). Higher microbiome diversity and concentrations of 2° BAs at EOT were associated with both lower rCDI and higher SCR rates. RDZ was well tolerated (pts with ≥ 1 TEAE: RDZ 36.4% vs VAN 35.5%, treatment discontinuation due to TEAE: RDZ 0.8% vs. VAN 2.9%). Conclusion RDZ was effective for sustained clinical response and safe for the treatment of patients with CDI. This was most notable in pts not receiving antibiotics. Compared to VAN, RDZ patients had faster recovery of fecal 2° BA, consistent with the preservation of microbiome diversity, resulting in a significantly lower rate of rCDI. Disclosures Pablo C. Okhuysen, MD, AstraZeneca: Stocks/Bonds|Beam Therapeutics: Stocks/Bonds|Biontech: Stocks/Bonds|Deinove: Grant/Research Support|Ferring: Advisor/Consultant|Glaxo Smith Kleine: Stocks/Bonds|Johnson and Johnson: Stocks/Bonds|Melinta: Grant/Research Support|Merck Sharp & Dohme Corp: Grant/Research Support|Moderna: Stocks/Bonds|Napo Pharmaceuticals: Advisor/Consultant|Napo Pharmaceuticals: Grant/Research Support|Novavax: Stocks/Bonds|Pfizer: Stocks/Bonds|Summit: Advisor/Consultant|Summit: Grant/Research Support Kevin W. Garey, PharmD, MS, Acurx: Grant/Research Support|cidara: Advisor/Consultant|cidara: Grant/Research Support|Paratek: Grant/Research Support|Seres Health: Grant/Research Support|Summit: Grant/Research Support Thomas J. Louie, MD, adiso therapeutics: Advisor/Consultant|adiso therapeutics: Grant/Research Support|crestone: Advisor/Consultant|crestone: Grant/Research Support|Finch: Advisor/Consultant|Finch: Grant/Research Support|Seres Therapeutics: Advisor/Consultant|Seres Therapeutics: Grant/Research Support|Seres Therapeutics: Honoraria|summit plc: Grant/Research Support|vedanta biosciences: Advisor/Consultant|vedanta biosciences: Grant/Research Support Jianling LI, MS, Abbott: Stocks/Bonds|Abbvie: Stocks/Bonds|ALX Oncology: Stocks/Bonds|BioNTech: Stocks/Bonds|Bluebird Bio: Stocks/Bonds|Cytokinetics: Stocks/Bonds|I-Mab: Stocks/Bonds|Johnson & Johnson: Stocks/Bonds|Moderna: Stocks/Bonds|TG Therapeutics: Stocks/Bonds Esther Duperchy, PhD, Summit Plc: Employee Jose G. Montoya, MD, Summit: Honoraria|Summit: Stocks/Bonds Lori Styles, MD, Abbvie: Stocks/Bonds|Summit Therapeutics: employee|Summit Therapeutics: Stocks/Bonds Fong Clow, Sc. D, Summit Therapeutics: Employee Danelle James, MD, Summit Therapeutics: Employee.

Image 1: A Little Free Library. Image credit: Nadine Kozak.IntroductionLittle Free Libraries give people a reason to stop and exchange things they love: books. It seemed like a really good way to build a sense of community.Dannette Lank, Little Free Library steward, Whitefish Bay, Wisconsin, 2013 (Rumage)Against a backdrop of stagnant literacy rates and enduring perceptions of urban decay and the decline of communities in cities (NCES, “Average Literacy”; NCES, “Average Prose”; Putnam 25; Skogan 8), legions of Little Free Libraries (LFLs) have sprung up across the United States between 2009 and the present. LFLs are small, often homemade structures housing books and other physical media for passersby to choose a book to take or leave a book to share with others. People have installed the structures in front of homes, schools, libraries, churches, fire and police stations, community gardens, and in public parks. There are currently 50,000 LFLs around the world, most of which are in the continental United States (Aldrich, “Big”). LFLs encompass building in multiple senses of the term; LFLs are literally tiny buildings to house books and people use the structures for building neighbourhood social capital. The organisation behind the movement cites “building community” as one of its three core missions (Little Free Library). Rowan Moore, theorising humans’ reasons for building, argues desire and emotion are central (16). The LFL movement provides evidence for this claim: stewards erect LFLs based on hope for increased literacy and a desire to build community through their altruistic actions. This article investigates how LFLs build urban community and explores barriers to the endeavour, specifically municipal building and right of way ordinances used in attempts to eradicate the structures. It also examines local responses to these municipal actions and potential challenges to traditional public libraries brought about by LFLs, primarily the decrease of visits to public libraries and the use of LFLs to argue for defunding of publicly provided library services. The work argues that LFLs build community in some places but may threaten other community services. This article employs qualitative content analysis of 261 stewards’ comments about their registered LFLs on the organisation’s website drawn from the two largest cities in a Midwestern state and an interview with an LFL steward in a village in the same state to analyse how LFLs build community. The two cities, located in the state where the LFL movement began, provide a cross section of innovators, early adopters, and late adopters of the book exchanges, determined by their registered charter numbers. Press coverage and municipal documents from six cities across the US gathered through a snowball sample provide data about municipal challenges to LFLs. Blog posts penned by practising librarians furnish some opinions about the movement. This research, while not a representative sample, identifies common themes and issues around LFLs and provides a basis for future research.The act of building and curating an LFL is a representation of shared beliefs about literacy, community, and altruism. Establishing an LFL is an act of civic participation. As Nico Carpentier notes, while some civic participation is macro, carried out at the level of the nation, other participation is micro, conducted in “the spheres of school, family, workplace, church, and community” (17). Ruth H. Landman investigates voluntary activities in the city, including community gardening, and community bakeries, and argues that the people associated with these projects find themselves in a “denser web of relations” than previously (2). Gretchen M. Herrmann argues that neighbourhood garage sales, although fleeting events, build an enduring sense of community amongst participants (189). Ray Oldenburg contends that people create associational webs in what he calls “great good places”; third spaces separate from home and work (20-21). Little Free Libraries and Community BuildingEmotion plays a central role in the decision to become an LFL steward, the person who establishes and maintains the LFL. People recount their desire to build a sense of community and share their love of reading with neighbours (Charter 4684; Charter 8212; Charter 9437; Charter 9705; Charter 16561). One steward in the study reported, “I love books and I want to be able to help foster that love in our neighbourhood as well” (Charter 4369). Image 2: A Little Free Library, bench, water fountain, and dog’s water bowl for passersby to enjoy. Image credit: Nadine Kozak.Relationships and emotional ties are central to some people’s decisions to have an LFL. The LFL website catalogues many instances of memorial LFLs, tributes to librarians, teachers, and avid readers. Indeed, the first Little Free Library, built by Todd Bol in 2009, was a tribute to his late mother, a teacher who loved reading (“Our History”). In the two city study area, ten LFLs are memorials, allowing bereaved families to pass on a loved one’s penchant for sharing books and reading (Charter 1235; Charter 1309; Charter 4604; Charter 6219; Charter 6542; Charter 6954; Charter 10326; Charter 16734; Charter 24481; Charter 30369). In some cases, urban neighbours come together to build, erect, and stock LFLs. One steward wrote: “Those of us who live in this friendly neighborhood collaborated to design[,] build and paint a bungalow themed library” to match the houses in the neighbourhood (Charter 2532). Another noted: “Our neighbor across the street is a skilled woodworker, and offered to build the library for us if we would install it in our yard and maintain it. What a deal!” (Charter 18677). Community organisations also install and maintain LFLs, including 21 in the study population (e.g. Charter 31822; Charter 27155).Stewards report increased communication with neighbours due to their LFLs. A steward noted: “We celebrated the library’s launch on a Saturday morning with neighbors of all ages. We love sitting on our front porch and catching up with the people who stop to check out the books” (Charter 9673). Another exclaimed:within 24 hours, before I had time to paint it, my Little Free Library took on a life of its own. All of a sudden there were lots of books in it and people stopping by. I wondered where these books came from as I had not put any in there. Little kids in the neighborhood are all excited about it and I have met neighbors that I had never seen before. This is going to be fun! (Charter 15981)LFLs build community through social interaction and collaboration. This occurs when neighbours come together to build, install, and fill the structures. The structures also open avenues for conversation between neighbours who had no connection previously. Like Herrmann’s neighbourhood garage sales, LFLs create and maintain social ties between neighbours and link them by the books they share. Additionally, when neighbours gather and communicate at the LFL structure, they create a transitory third space for “informal public life”, where people can casually interact at a nearby location (Oldenburg 14, 288).Building Barriers, Creating CommunityThe erection of an LFL in an urban neighbourhood is not, however, always a welcome sight. The news analysis found that LFLs most often come to the attention of municipal authorities via citizen complaints, which lead to investigations and enforcement of ordinances. In Kansas, a neighbour called an LFL an “eyesore” and an “illegal detached structure” (Tapper). In Wisconsin, well-meaning future stewards contacted their village authorities to ask about rules, inadvertently setting off a six-month ban on LFLs (Stingl; Rumage). Resulting from complaints and inquiries, municipalities regulated, and in one case banned, LFLs, thus building barriers to citizens’ desires to foster community and share books with neighbours.Municipal governments use two major areas of established code to remove or prohibit LFLs: ordinances banning unapproved structures in residents’ yards and those concerned with obstructions to right of ways when stewards locate the LFLs between the public sidewalk and street.In the first instance, municipal ordinances prohibit either front yard or detached structures. Controversies over these ordinances and LFLs erupted in Whitefish Bay, Wisconsin, in 2012; Leawood, Kansas, in 2014; Shreveport, Louisiana, in 2015; and Dallas, Texas, in 2015. The Village of Whitefish Bay banned LFLs due to an ordinance prohibiting “front yard structures,” including mailboxes (Sanburn; Stingl). In Leawood, the city council argued that an LFL, owned by a nine-year-old boy, violated an ordinance that forbade the construction of any detached structures without city council permission. In Shreveport, the stewards of an LFL received a cease and desist letter from city council for having an “accessory structure” in the front yard (LaCasse; Burris) and Dallas officials knocked on a steward’s front door, informing her of a similar breach (Kellogg).In the second instance, some urban municipalities argued that LFLs are obstructions that block right of ways. In Lincoln, Nebraska, the public works director noted that the city “uses the area between the sidewalk and the street for snow storage in the winter, light poles, mailboxes, things like that.” The director continued: “And I imagine these little libraries are meant to congregate people like a water cooler, but we don’t want people hanging around near the road by the curb” (Heady). Both Lincoln in 2014 and Los Angeles (LA), California, in 2015, cited LFLs for obstructions. In Lincoln, the city notified the Southminster United Methodist Church that their LFL, located between the public sidewalk and street, violated a municipal ordinance (Sanburn). In LA, the Bureau of Street Services notified actor Peter Cook that his LFL, situated in the right of way, was an “obstruction” that Cook had to remove or the city would levy a fine (Moss). The city agreed at a hearing to consider a “revocable permit” for Cook’s LFL, but later denied its issuance (Condes).Stewards who found themselves in violation of municipal ordinances were able to harness emotion and build outrage over limits to individuals’ ability to erect LFLs. In Kansas, the stewards created a Facebook page, Spencer’s Little Free Library, which received over 31,000 likes and messages of support. One comment left on the page reads: “The public outcry will force those lame city officials to change their minds about it. Leave it to the stupid government to rain on everybody’s parade” (“Good”). Children’s author Daniel Handler sent a letter to the nine-year-old steward, writing as Lemony Snicket, “fighting against librarians is immoral and useless in the face of brave and noble readers such as yourself” (Spencer’s). Indeed, the young steward gave a successful speech to city hall arguing that the body should allow the structures because “‘lots of people in the neighborhood used the library and the books were always changing. I think it’s good for Leawood’” (Bauman). Other local LFL supporters also attended council and spoke in favour of the structures (Harper). In LA, Cook’s neighbours started a petition that gathered over 100 signatures, where people left comments including, “No to bullies!” (Lopez). Additionally, neighbours gathered to discuss the issue (Dana). In Shreveport, neighbours left stacks of books in their front yards, without a structure housing them due to the code banning accessory structures. One noted, “I’m basically telling the [Metropolitan Planning Commission] to go sod off” (Friedersdorf; Moss). LFL proponents reacted with frustration and anger at the perceived over-reach of the government toward harmless LFLs. In addition to the actions of neighbours and supporters, the national and local press commented on the municipal constraints. The LFL movement has benefitted from a significant amount of positive press in its formative years, a press willing to publicise and criticise municipal actions to thwart LFL development. Stewards’ struggles against municipal bureaucracies building barriers to LFLs makes prime fodder for the news media. Herbert J. Gans argues an enduring value in American news is “the preservation of the freedom of the individual against the encroachments of nation and society” (50). The juxtaposition of well-meaning LFL stewards against municipal councils and committees provided a compelling opportunity to illustrate this value.National media outlets, including Time (Sanburn), Christian Science Monitor (LaCasse), and The Atlantic, drew attention to the issue. Writing in The Atlantic, Conor Friedersdorf critically noted:I wish I was writing this to merely extol this trend [of community building via LFLs]. Alas, a subset of Americans are determined to regulate every last aspect of community life. Due to selection bias, they are overrepresented among local politicians and bureaucrats. And so they have power, despite their small-mindedness, inflexibility, and lack of common sense so extreme that they’ve taken to cracking down on Little Free Libraries, of all things. (Friedersdorf, n.p.)Other columnists mirrored this sentiment. Writing in the LA Times, one commentator sarcastically wrote that city officials were “cracking down on one of the country’s biggest problems: small community libraries where residents share books” (Schaub). Journalists argued this was government overreach on non-issues rather than tackling larger community problems, such as income inequality, homelessness, and aging infrastructure (Solomon; Schaub). The protests and negative press coverage led to, in the case of the municipalities with front yard and detached structure ordinances, détente between stewards and councils as the latter passed amendments permitting and regulating LFLs. Whitefish Bay, Leawood, and Shreveport amended ordinances to allow for LFLs, but also to regulate them (Everson; Topil; Siegel). Ordinances about LFLs restricted their number on city blocks, placement on private property, size and height, as well as required registration with the municipality in some cases. Lincoln officials allowed the church to relocate the LFL from the right of way to church property and waived the $500 fine for the obstruction violation (Sanburn). In addition to the amendments, the protests also led to civic participation and community building including presentations to city council, a petition, and symbolic acts of defiance. Through this protest, neighbours create communities—networks of people working toward a common goal. This aspect of community building around LFLs was unintentional but it brought people together nevertheless.Building a Challenge to Traditional Libraries?LFL marketing and communication staff member Margaret Aldrich suggests in The Little Free Library Book that LFLs are successful because they are “gratifyingly doable” projects that can be accomplished by an individual (16). It is this ease of building, erecting, and maintaining LFLs that builds concern as their proliferation could challenge aspects of library service, such as public funding and patron visits. Some professional librarians are in favour of the LFLs and are stewards themselves (Charter 121; Charter 2608; Charter 9702; Charter 41074; Rumage). Others envision great opportunities for collaboration between traditional libraries and LFLs, including the library publicising LFLs and encouraging their construction as well as using LFLs to serve areas without, or far from, a public library (Svehla; Shumaker). While lauding efforts to build community, some professional librarians question the nomenclature used by the movement. They argue the phrase Little Free Libraries is inaccurate as libraries are much more than random collections of books. Instead, critics contend, the LFL structures are closer to book swaps and exchanges than actual libraries, which offer a range of services such as Internet access, digital materials, community meeting spaces, and workshops and programming on a variety of topics (American Library Association; Annoyed Librarian). One university reference and instruction librarian worries about “the general public’s perception and lumping together of little free libraries and actual ‘real’ public libraries” (Hardenbrook). By way of illustration, he imagines someone asking, “‘why do we need our tax money to go to something that can be done for FREE?’” (Hardenbrook). Librarians holding this perspective fear the movement might add to a trend of neoliberalism, limiting or ending public funding for libraries, as politicians believe that the localised, individual solutions can replace publicly funded library services. This is a trend toward what James Ferguson calls “responsibilized” citizens, those “deployed to produce governmentalized results that do not depend on direct state intervention” (172). In other countries, this shift has already begun. In the United Kingdom (UK), governments are devolving formerly public services onto community groups and volunteers. Lindsay Findlay-King, Geoff Nichols, Deborah Forbes, and Gordon Macfadyen trace the impacts of the 2012 Localism Act in the UK, which caused “sport and library asset transfers” (12) to community and volunteer groups who were then responsible for service provision and, potentially, facility maintenance as well. Rather than being in charge of a “doable” LFL, community groups and volunteers become the operators of much larger facilities. Recent efforts in the US to privatise library services as governments attempt to cut budgets and streamline services (Streitfeld) ground this fear. Image 3: “Take a Book, Share a Book,” a Little Free Library motto. Image credit: Nadine Kozak. LFLs might have real consequences for public libraries. Another potential unintended consequence of the LFLs is decreasing visits to public libraries, which could provide officials seeking to defund them with evidence that they are no longer relevant or necessary. One LFL steward and avid reader remarked that she had not used her local public library since 2014 because “I was using the Little Free Libraries” (Steward). Academics and librarians must conduct more research to determine what impact, if any, LFLs are having on visits to traditional public libraries. ConclusionLittle Free Libraries across the United States, and increasingly in other countries, have generated discussion, promoted collaboration between neighbours, and led to sharing. In other words, they have built communities. This was the intended consequence of the LFL movement. There, however, has also been unplanned community building in response to municipal threats to the structures due to right of way, safety, and planning ordinances. The more threatening concern is not the municipal ordinances used to block LFL development, but rather the trend of privatisation of publicly provided services. While people are celebrating the community built by the LFLs, caution must be exercised lest central institutions of the public and community, traditional public libraries, be lost. Academics and communities ought to consider not just impact on their local community at the street level, but also wider structural concerns so that communities can foster many “great good places”—the Little Free Libraries and traditional public libraries as well.ReferencesAldrich, Margaret. “Big Milestone for Little Free Library: 50,000 Libraries Worldwide.” Little Free Library. Little Free Library Organization. 4 Nov. 2016. 25 Feb. 2017 <https://littlefreelibrary.org/big-milestone-for-little-free-library-50000-libraries-worldwide/>.Aldrich, Margaret. The Little Free Library Book: Take a Book, Return a Book. Minneapolis, MN: Coffee House Press, 2015.Annoyed Librarian. “How to Protect Little Free Libraries.” Library Journal Blog 9 Jul. 2015. 26 Mar. 2017 <http://lj.libraryjournal.com/blogs/annoyedlibrarian/2015/07/09/how-to-protect-little-free-libraries/>.American Library Association. “Public Library Use.” State of America’s Libraries: A Report from the American Library Association (2015). 25 Feb. 2017 <http://www.ala.org/tools/libfactsheets/alalibraryfactsheet06>.Bauman, Caroline. “‘Little Free Libraries’ Legal in Leawood Thanks to 9-year-old Spencer Collins.” The Kansas City Star 7 Jul. 2014. 25 Feb. 2017 <http://www.kansascity.com/news/politics-government/article687562.html>.Burris, Alexandria. “First Amendment Issues Surface in Little Free Library Case.” Shreveport Times 5 Feb. 2015. 25 Feb. 2017 <http://www.shreveporttimes.com/story/news/local/2015/02/05/expert-use-zoning-law-clashes-first-amendment/22922371/>.Carpentier, Nico. Media and Participation: A Site of Ideological-Democratic Struggle. Bristol: Intellect, 2011.Charter 121. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 1235. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 1309. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 2532. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 2608. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 4369. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 4604. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 4684. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 6219. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 6542. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 6954. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 8212. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 9437. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 9673. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 9702. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 9705. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 10326. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 15981. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 16561. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 16734. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 18677. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 24481. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 27155. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 30369. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 31822. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Charter 41074. “The World Map.” Little Free Library (2017). 26 Mar. 2017 <https://littlefreelibrary.org/ourmap/>.Condes, Yvonne. “Save the Little Library!” MomsLA 10 Aug. 2015. 25 Feb. 2017 <http://momsla.com/save-the-micro-library/>.Dana. “The Tenn-Mann Library Controversy, Part 3.” Read with Dana (30 Jan. 2015). 25 Feb. 2017 <https://readwithdana.wordpress.com/2015/01/30/the-tenn-mann-library-controversy-part-three/>.Everson, Jeff. “An Ordinance to Amend and Reenact Chapter 106 of the Shreveport Code of Ordinances Relative to Outdoor Book Exchange Boxes, and Otherwise Providing with Respect Thereto.” City of Shreveport, Louisiana 9 Oct. 2015. 25 Feb. 2017 <http://ftpcontent4.worldnow.com/ksla/pdf/LFLordinance.pdf>.Ferguson, James. “The Uses of Neoliberalism.” Antipode 41.S1 (2009): 166-84.Findlay-King, Lindsay, Geoff Nichols, Deborah Forbes, and Gordon Macfadyen. “Localism and the Big Society: The Asset Transfer of Leisure Centres and Libraries—Fighting Closures or Empowering Communities.” Leisure Studies (2017): 1-13.Friedersdorf, Conor. “The Danger of Being Neighborly without a Permit.” The Atlantic 20 Feb. 2015. 25 Feb. 2017 <https://www.theatlantic.com/national/archive/2015/02/little-free-library-crackdown/385531/>.Gans, Herbert J. Deciding What’s News: A Study of CBS Evening News, NBC Nightly News, Newsweek, and Time. Evanston, IL: Northwestern University Press, 2004.“Good Luck Spencer.” Spencer’s Little Free Library Facebook Page 25 Jun. 2014. 26 Mar. 2017 <https://www.facebook.com/Spencerslittlefreelibrary/photos/pcb.527531327376433/527531260709773/?type=3>.Hardenbrook, Joe. “A Little Rant on Little Free Libraries (AKA Probably an Unpopular Post).” Mr. Library Dude (9 Apr. 2014). 25 Feb. 2017 <https://mrlibrarydude.wordpress.com/2014/04/09/a-little-rant-on-little-free-libraries-aka-probably-an-unpopular-post/>.Harper, Deb. “Minutes.” The Leawood City Council 7 Jul. 2014. <http://www.leawood.org/pdf/cc/min/07-07-14.pdf>. Heady, Chris. “City Wants Church to Move Little Library.” Lincoln Journal Star 9 Jul. 2014. 25 Feb. 2017 <http://journalstar.com/news/local/city-wants-church-to-move-little-library/article_7753901a-42cd-5b52-9674-fc54a4d51f47.html>. Herrmann, Gretchen M. “Garage Sales Make Good Neighbors: Building Community through Neighborhood Sales.” Human Organization 62.2 (2006): 181-191.Kellogg, Carolyn. “Officials Threaten to Destroy a Little Free Library in Texas.” Los Angeles Times (1 Oct. 2015). 25 Feb. 2017 <http://www.latimes.com/books/jacketcopy/la-et-jc-little-free-library-texas-20150930-story.html>.LaCasse, Alexander. “Why Are Some Cities Cracking Down on Little Free Libraries.” Christian Science Monitor (5 Feb. 2015). 25 Feb. 2017 <http://www.csmonitor.com/Books/chapter-and-verse/2015/0205/Why-are-some-cities-cracking-down-on-little-free-libraries>.Landman, Ruth H. Creating the Community in the City: Cooperatives and Community Gardens in Washington, DC Westport, CT: Bergin & Garvey, 1993. Little Free Library. Little Free Library Organization (2017). 25 Feb. 2017 <https://littlefreelibrary.org/>.Lopez, Steve. “Actor’s Curbside Libraries Is a Smash—for Most People.” LA Times 3 Feb. 2015. 25 Feb. 2017 <http://www.latimes.com/local/california/la-me-0204-lopez-library-20150204-column.html>.Moore, Rowan. Why We Build: Power and Desire in Architecture. New York: Harper Design, 2013.Moss, Laura. “City Zoning Laws Target Little Free Libraries.” Mother Nature Network 25 Aug. 2015. 25 Feb. 2017 <http://www.mnn.com/lifestyle/arts-culture/stories/city-zoning-laws-target-little-free-libraries>.National Center for Education Statistics (NCES). Average Literacy and Numeracy Scale Scores of 25- to 65-Year Olds, by Sex, Age Group, Highest Level of Educational Attainment, and Country of Other Education System: 2012, table 604.10. 25 Feb. 2017 <https://nces.ed.gov/programs/digest/d15/tables/dt15_604.10.asp?current=yes>.National Center for Education Statistics (NCES). Average Prose, Document, and Quantitative Literacy Scores of Adults: 1992 and 2003. National Assessment of Adult Literacy. 25 Feb. 2017 <https://nces.ed.gov/naal/kf_demographics.asp>.Oldenburg, Ray. The Great Good Place: Cafés, Coffee Shops, Bookstores, Bars, Hair Salons, and Other Hangouts at the Heart of a Community. New York: Marlowe & Company, 1999.“Our History.” Little Free Library. Little Free Library Organization (2017). 25 Feb. 2017 <https://littlefreelibrary.org/ourhistory/>.Putnam, Robert D. Bowling Alone: The Collapse and Revival of American Community. New York: Simon & Schuster, 2001.Rumage, Jeff. “Little Free Libraries Now Allowed in Whitefish Bay.” Whitefish Bay Patch (8 May 2013). 25 Feb. 2017 <http://patch.com/wisconsin/whitefishbay/little-free-libraries-now-allowed-in-whitefish-bay>.Sanburn, Josh. “What Do Kansas and Nebraska Have against Small Libraries?” Time 10 Jul. 2014. 25 Feb. 2017 <http://time.com/2970649/tiny-libraries-violating-city-ordinances/>.Schaub, Michael. “Little Free Libraries on the Wrong Side of the Law.” LA Times 4 Feb. 2015. 25 Feb. 2017 <http://www.latimes.com/books/jacketcopy/la-et-jc-little-free-libraries-on-the-wrong-side-of-the-law-20150204-story.html>.Shumaker, David. “Public Libraries, Little Free Libraries, and Embedded Librarians.” The Embedded Librarian (28 April 2014) 26 Mar. 2017 <https://embeddedlibrarian.com/2014/04/28/public-libraries-little-free-libraries-and-embedded-librarians/>.Siegel, Julie. “An Ordinance to Amend Section 16.13 of the Municipal Code with Regard to Exempt Certain Little Free Libraries from Front Yard Setback Requirements.” Village of Whitefish Bay, Wisconsin (5 Aug. 2013).Skogan, Wesley G. Police and Community in Chicago: A Tale of Three Cities. New York: Oxford University Press, 2006.Solomon, Dan. “Dallas Is Regulating ‘Little Free Libraries’ for Some Reason.” Texas Monthly (14 Sept. 2016). 25 Feb. 2017 <http://www.texasmonthly.com/the-daily-post/dallas-regulating-little-free-libraries-reason/>.“Spencer’s Little Free Library.” Facebook 15 Jul. 2014. 25 Feb. 2017 <https://www.facebook.com/Spencerslittlefreelibrary/photos/pcb.527531327376433/527531260709773/?type=3>.Steward, M. Personal Interview. 7 Feb. 2017.Stingl, Jim. “Village Slaps Endnote on Little Libraries.” Milwaukee Journal Sentinel 11 Nov. 2012: 1B, 7B.Streitfeld, David. “Anger as a Private Company Takes over Libraries.” The New York Times (26 Sept. 2010). 25 Feb. 2017 <http://www.nytimes.com/2010/09/27/business/27libraries.html>.Svehla, Louise. “Little Free Libraries—The Possibilities Are Endless.” Public Libraries Online (8 Mar. 2013). 25 Feb. 2017 <http://publiclibrariesonline.org/2013/03/little-free-libraries-the-possibilities-are-endless/>.Tapper, Jake. “Boy Fights Council to Save His Library.” CNN 4 Jul. 2014. 25 Feb. 2017 <http://thelead.blogs.cnn.com/2014/07/04/boy-fights-to-save-his-library/>.Topil, Greg. “Little Free Libraries in Lincoln.” City of Lincoln, Nebraska (n.d.). 25 Feb. 2017 <http://lincoln.ne.gov/City/pworks/engine/row/little-library.htm>.

In a post September 11 era “the fight”, as a cultural construct, could hardly be more pertinent. We are seemingly forever poised on the edge of controversial U.S. led attacks on wayward Middle Eastern states and unexamined oppositions between the concepts of ‘good’ and ‘evil’ are evoked as valid justifications for battle. Our leaders muster us into wars of vigilance and national cohesion against unseen, unknown and uncomprehended terrorists hiding where communists once lurked under our beds. The articles in this issue examine fights in terms of media strategies and cultural divides in a range of contexts. Our feature article is a work of a fiction, an extract from the sharply beautiful novella Moving by Julienne van Loon, describing a fight between friends, maybe lovers. Set against the harsh backdrop of urban working-class Sydney, the fight here is personal, a spontaneous response to a hurt done, an expression of anger and frustration. Loon’s work explores the nature of physical struggle, the bond of shared physicality between opponents and the potential for frustration and resolution. Perhaps a little akin to Fight Club in its affirmation of the distinctive intensity of violent contact Loon shifts the nuances to a female character living in a male dominated environment. Mark Mullen’s detailed analysis of the politics of death in computer games is a timely intervention into the debates over the relationship between ‘virtual’ and ‘real world’ violence. Contrary to the conservative and neo-Marxist theses that games routinise killing and desensitise us to violence, his work suggests that gamers regularly make conscious choices that are unavailable to people in “real life:” most importantly, gamers can sometimes choose to put death or killing on hold in order to find alternatives. Mullen goes so far as to propose that gaming literacy may even provide a set of ethical tools for avoiding the acute situations that, it seems, “inevitably” result in violence or war. Meanwhile, back in the “real” world, ex- war correspondent Chris Vaughan quotes the news maxim “If it bleeds, it leads” to open his often personal account of the journalistic imperative to get up close to violence. Vaughan’s essay reminds us that the news media’s focus on the acutely violent, explosive events that signify “ war” does not proceed directly from an amorphous “ideology” that simply expresses and safeguards the material interests of dominant groups. Rather, such representations are at the same time selected and shaped according to the conventions and constraints (whether economic or political) of the professions that produce them. “To be nobody-but-yourself—in a world which is doing its best, night and day, to make you everybody else—means to fight the hardest battle which any human being can fight; and never stop fighting.” (e. e. cummings) It is of course important to remember (as our feature story demonstrates) that fights can also be personal, local, part of everyday life—and these ordinary fights require as much rhetorical justification as any war. Paul Scott’s analysis of the fights between surfers at Australian surf breaks—superficially enacted as struggles for territory, but also functioning to police the boundaries of what may seem to participants of surf cultures to be the last vestige of subcultural authenticity—refracts two key problems of postmodern Western culture through the lens of surfing: firstly, he offers insight into how citizens and consumers manage the tensions between localism and (corporate) globalism and, secondly, his discussion of surf rage throws the articulation between normative masculinity and physical aggression into stark relief. “In accordance with our principles of free enterprise and healthy competition, I'm going to ask you two to fight to the death for it.” (Monty Python) Moving from the personal to the political, Louis Kaplan examines John Lennon and Yoko Ono’s astute blend of radicalism, humour, stylistic flair and media negotiation in their battle for peace during the Vietnam War. The fight is depicted as a marketing campaign by the celebrity couple aimed at promoting peace as a desirable ‘product’ to the public and politicians whilst weathering criticism that it was mere self-promotion. Kaplan reveals their fight to be composed of both struggle and promotion of self and stance, hence making the personal political. Looking to a more recent campaign in the music world Axel Bruns scrutinizes the 2002 legal battles over royalty rates between America’s powerful recording industry (RIAA) and the emerging Webcasters of online radio, battling to survive and serve their audiences with alternative music fare. Bruns traces the stages in the campaign and studies the rival hostilities and motivations. This issue has a substantial concentration of articles devoted to the film Fight Club. Our authors have found it instructive to return to that nasty little fable about characters that turn to the fight as a way of assuaging an obscure sense of alienation from contemporary, capitalist society. They were all interested in how the particularly blokey sense of anomie depicted in Fight Club continues the tradition of Falling Down (Dir. Joel Schumacher, 1993) in creating a permanent sense of crisis about a perceived “masculine impotence in the face of a loss suffered but not remembered.” (Gatens 86) Melissa Iocco examines how this sense of crisis, this sense of men being divided from themselves, is somatically represented in spectacular screen displays of suffering, of damage. She looks at how fighting writes protest and resistance on the male—through the scars, the bleeding, and the destruction inflicted by fighting. She also reflects on how analogous crises may be displaying themselves culturally off-screen, as the kind of talented, disaffected young men responsible for flying planes into the Twin Towers seem to be helping remake the world we inhabit in the image of project mayhem. Kate Greenwood pursues the question of how doing things to the male body inflects the construction of masculine identity construction. She discusses Tyler’s assertion that the “real pain,” the total temporary immanence experienced by the men engaged in fighting, is a path back to an “authentic” experience of masculinity. Tracy Caldwell turns from exteriorities to interiorities, to conduct a psychoanalytic reading of how the film exploits grotesque confusion of boundaries and of gender identities to dramatise a contemporary struggle surrounding the construction of masculine subjectivity. She uses Creed’s readings of Kristeva to analyse how the ‘abject’ is used in Fight Club as an urgent warning about the danger of not finding a way to repair masculine identity. Taken together, these three articles illustrate how Fight Club extends a grand old American tradition of using the fight, the exchange of blows, as a way of constructing identity. Faulkner’s Addie lays out its virtues in As I Lay Dying: I would look forward to the times when they faulted, so I could whip them. When the switch fell I could feel it upon my flesh; when it welted and ridged it was my blood that ran, and I would think with each blow of the switch: Now you are aware of me! Now I am something in your secret and selfish life, who have marked your blood with my own for ever and ever. That the ‘fight’ described here is hugely asymmetrical—one party whips and the other is whipped—does not diminish the effectiveness of the assault for the one using it as a device for constructing identity, and asserting a particular relationship between the parties to the ‘fight.’ Perhaps this is why George W. Bush appears to be so eager, at the moment, to find that Iraq may have “faulted”… There is, in this issue, a thunderous silence about this most pressing and obvious fight. We were somewhat surprised, given the number and diversity of submissions to this issue, that none chose to directly discuss the politics of the U.S. led war on terrorism and campaign to attack Iraq. It seems a ready example of the construct of a ‘fight’ involving the framing of an opposition, the build up and exchange of hostilities and the development of a cultural discourse of security, national cohesion and identity. Yet perhaps this fight’s proximity renders it too immediate and disturbing for comfort, accentuating the closeness, the almost inevitable physical and deep emotional resonance of fights themselves. Which is why Julienne van Loon’s direct, credible and evocative prose seems such a good place to begin. Let’s rumble. Works Cited Gatens, Moira. "Corporeal Representation in/and the Body Politic." Cartographies: Poststructuralism and the Mapping of Bodies and Spaces. Rosalyn Diprose and Robyn Ferrell. eds. Sydney: Allen & Unwin, 1991. Citation reference for this article Substitute your date of access for Dn Month Year etc... MLA Style Burgess, Jean; McEntee, Joy and Nelms, Emma. "How to Pick a Fight" M/C: A Journal of Media and Culture 6.1 (2003). Dn Month Year < http://www.media-culture.org.au/0302/01-editorial.php>. APA Style Burgess, J., McEntee, J. & Nelms, E., (2003, Feb 26). How to Pick a Fight. M/C: A Journal of Media and Culture, 6,(1). Retrieved Month Dn, Year, from http://www.media-culture.org.au/0302/01-editorial.html

My title comes from anthropologist Mary Douglas’ assertion that dirt, when understood as “matter out of place” simultaneously implies both the existence and the contravention of an established order or system and that this in turn establishes dirt as symbolic (35). Further, Phyllis Palmer has written that dirt is “a principal means to arrange culture” (139). This paper suggests both that dirt has a function in cultural constructions of gender and that action films featuring a female protagonist provide a fertile site for investigation. Normative white femininity traditionally eschews direct contact with dirt thus bringing into play interactions between work and gender. This article begins to question what it is that the appearance and disappearance of dirt signifies for the understanding of femininities in the particular cultural practice commonly recognised as action films. One need only observe advertisements for cleaning products or compare shelf space taken up by personal hygiene items for men to that taken by similar items for women to understand the continuation of the gendered nature of cleanliness. Women are expected to keep not only the environment but themselves clean as a measure of their femininity. Indeed, obsessive cleanliness formed a part of Friedan’s feminine mystique. Not only must transgressive women such as the eponymous protagonists of Thelma and Louise die, but also they must die unwashed, driving a dirty car into a hole in the ground, being pursued by a dust cloud. Having trespassed on the masculine territory of self-defence and free movement this can be their only end. Linda Williams points out that all they are guilty of is of behaving “in the time-honoured tradition of most American heroes, violently and without reflection” (27). Further, the women who suicide into the Grand Canyon at the end of the film are distanced from the two in the bright, iconic, self portrait from the beginning and it is this shiny vision of femininity that was central to the film’s promotion. They have driven west, away from civilised society, ultimately facing e what “the western still tells us and what we still continue to buy…that reality is blood and dust and death and a cold wind blowing” (Tompkins 99). The signs of the exhausting exertion of sustained non-capitulation and the adherent grime of the road they have travelled are plain on their faces. Like Ripley, once they are truly fighting alone they begin to accumulate layers of dirt on their skin. Unlike Ripley they can neither return from their nightmare nor separate themselves from their actions. Relentlessly pursued, they cannot stop to wash off the dirt just as they cannot eschew responsibility for what they have done. They have become dressed in the dust of road, no longer on it but of it. Another discourse is in operation in conjunction with the gender skewing discourse of dirt. Whenever one addresses a change in the colour of skin one engages with the discourse of race. Audiences are conditioned by Hollywood cinema to view non-Anglos in film as a potential threat. The darkening of the hero’s skin by dirt renders him or her as a perpetrator of violent acts. When the necessity for violence is over the hero cleans up (whitens up) to return to society. I am thinking here of the heroes of Stallone’s Rambo and Willis’s Die Hard series who followed Ripley. As their actions became increasingly violent they become progressively filthier. Rambo in particular, it is suggested, becomes more ‘primal’. I am not suggesting that filth is an issue involving gender, race or class in a simple way. Rather, these three issues are, as in most cases, intricately intertwined here too. By the time Sigourney Weaver’s Ripley traversed her first revenge narrative, Alien (1979) through the frontier of space, I would suggest that dirt had become a marker of the gendered action hero. The character of Ellen Ripley was originally written as a man, writer and director Ridley Scott asserting in the DVD commentary that the script was left unchanged when Weaver was cast. In Alien, Ripley plays the part of film theorist Carol Clover’s Final Girl. Clover writes that in slasher/horror films the survivor begins as the pursued, feminised victim of a male oppressor but in her ultimate triumph occupies the position of the traditional masculine avenger (35-37, 158-59). That is, she saves herself. It was new, too, that this ground breaking female hero battled against the monstrous femininities of the alien and ‘Mother’, the ship’s computer, to survive. Marking Ripley’s transgression into the strictly gendered territory of the solitarily courageous, increasingly frequent close-ups of her grimy hands and sweat-drenched face fill the screen. Alien begins in the pristine white of the sleep chamber reflecting the mainstream science fiction convention that contrasts the absence of dirt in the artificial space environment of the space ship with planet-based presence of dirt. It is Ripley who refuses to let the scouting party back onto the ship citing contamination procedure, underlining cleanliness and purity as a cultural ideal with women as its gatekeepers. The film ends in the white escape pod where Ripley immediately strips to her underwear, discarding the soiled outer layer of clothing. After one last ‘unexpected’ confrontation Ripley is shown clean and further feminised by the silk robe she is wearing and the soothing motion of stroking the cat (the only other survivor) on her lap. As she records her report she reverts to the role of Chorus, setting her apart from the action. Finally she is seen once more inside a sleep chamber as she was in the beginning. Returning to the gendered confines of ‘civilisation’ she must wash off the (masculine) signs of her struggle and return to sleep as if it were all a nightmare. Through Rambo, Die Hard’s McClane and other male action heroes the begrimed body has arguably become a signifier not only of survival but also of persistence and courage in the face of tremendous odds. Persistence and courage are gendered values. Thus, I would argue, its signifier, the dirty body, is similarly gendered. In Alien, Ellen Ripley, having triumphed through survival, reinstates her femininity (signified by cleanliness) and returns home. Ripley’s actions have been unobserved, her battlefield contained and her enemy (she thinks) proven tangible and finite. Isolated in space and relieved of being the object of the male gaze within her narrative by the deaths of her colleagues Ripley is free to begin a new discourse (and to found a new, female action tradition). Instead she takes a shower. References Alien. Dir. Ridley Scott. Perf. Sigourney Weaver. 20th Century Fox, 1979. DVD. 20th Century Fox Home Video, 1999. Clover, Carol J. Men, Women, and Chain Saws: Gender in the Modern Horror Film. Princeton, N.J.: Princeton UP, 1992. Douglas, Mary. Purity and Danger: An Analysis of the Concepts of Pollution and Taboo. London: Ark Paperbacks, 1966. Palmer, Phyllis. Domesticity and Dirt: Housewives and Domestic Servants in the United States, 1920-1945. Philadelphia: Temple UP, 1989. Tompkins, Jane. “Language and Landscape: An Ontology for the Western.” Artforum 28.6 (1990): 94-9. Williams, Linda. “What Makes a Woman Wander.” Film Quarterly 45.2 (1991-2): 27-28. Citation reference for this article MLA Style Wical, Carol. "Matter Out of Place: Reading Dirty Women." M/C Journal 9.5 (2006). echo date('d M. Y'); ?> <http://journal.media-culture.org.au/0610/10-wical.php>. APA Style Wical, C. (Nov. 2006) "Matter Out of Place: Reading Dirty Women," M/C Journal, 9(5). Retrieved echo date('d M. Y'); ?> from <http://journal.media-culture.org.au/0610/10-wical.php>.

IntroductionCulinary education in Ireland has long been influenced by culinary education being delivered in catering colleges in the United Kingdom (UK). Institutionalised culinary education started in Britain through the sponsorship of guild conglomerates (Lawson and Silver). The City & Guilds of London Institute for the Advancement of Technical Education opened its central institution in 1884. Culinary education in Ireland began in Kevin Street Technical School in the late 1880s. This consisted of evening courses in plain cookery. Dublin’s leading chefs and waiters of the time participated in developing courses in French culinary classics and these courses ran in Parnell Square Vocational School from 1926 (Mac Con Iomaire “The Changing”). St Mary’s College of Domestic Science was purpose built and opened in 1941 in Cathal Brugha Street. This was renamed the Dublin College of Catering in the 1950s. The Council for Education, Recruitment and Training for the Hotel Industry (CERT) was set up in 1963 and ran cookery courses using the City & Guilds of London examinations as its benchmark. In 1982, when the National Craft Curriculum Certification Board (NCCCB) was established, CERT began carrying out their own examinations. This allowed Irish catering education to set its own standards, establish its own criteria and award its own certificates, roles which were previously carried out by City & Guilds of London (Corr). CERT awarded its first certificates in professional cookery in 1989. The training role of CERT was taken over by Fáilte Ireland, the State tourism board, in 2003. Changing Trends in Cookery and Culinary Textbooks at DIT The Dublin College of Catering which became part of the Dublin Institute of Technology (DIT) is the flagship of catering education in Ireland (Mac Con Iomaire “The Changing”). The first DIT culinary award, was introduced in 1984 Certificate in Diet Cookery, later renamed Higher Certificate in Health and Nutrition for the Culinary Arts. On the 19th of July 1992 the Dublin Institute of Technology Act was enacted into law. This Act enabled DIT to provide vocational and technical education and training for the economic, technological, scientific, commercial, industrial, social and cultural development of the State (Ireland 1992). In 1998, DIT was granted degree awarding powers by the Irish state, enabling it to make major awards at Higher Certificate, Ordinary Bachelor Degree, Honors Bachelor Degree, Masters and PhD levels (Levels six to ten in the National Framework of Qualifications), as well as a range of minor, special purpose and supplemental awards (National NQAI). It was not until 1999, when a primary degree in Culinary Arts was sanctioned by the Department of Education in Ireland (Duff, The Story), that a more diverse range of textbooks was recommended based on a new liberal/vocational educational philosophy. DITs School of Culinary Arts currently offers: Higher Certificates Health and Nutrition for the Culinary Arts; Higher Certificate in Culinary Arts (Professional Culinary Practice); BSc (Ord) in Baking and Pastry Arts Management; BA (Hons) in Culinary Arts; BSc (Hons) Bar Management and Entrepreneurship; BSc (Hons) in Culinary Entrepreneurship; and, MSc in Culinary Innovation and Food Product Development. From 1942 to 1970, haute cuisine, or classical French cuisine was the most influential cooking trend in Irish cuisine and this is reflected in the culinary textbooks of that era. Haute cuisine has been influenced by many influential writers/chefs such as Francois La Varenne, Antoine Carême, Auguste Escoffier, Ferand Point, Paul Bocuse, Anton Mosiman, Albert and Michel Roux to name but a few. The period from 1947 to 1974 can be viewed as a “golden age” of haute cuisine in Ireland, as more award-winning world-class restaurants traded in Dublin during this period than at any other time in history (Mac Con Iomaire “The Changing”). Hotels and restaurants were run in the Escoffier partie system style which is a system of hierarchy among kitchen staff and areas of the kitchens specialising in cooking particular parts of the menu i.e sauces (saucier), fish (poissonnier), larder (garde manger), vegetable (legumier) and pastry (patissier). In the late 1960s, Escoffier-styled restaurants were considered overstaffed and were no longer financially viable. Restaurants began to be run by chef-proprietors, using plate rather than silver service. Nouvelle cuisine began in the 1970s and this became a modern form of haute cuisine (Gillespie). The rise in chef-proprietor run restaurants in Ireland reflected the same characteristics of the nouvelle cuisine movement. Culinary textbooks such as Practical Professional Cookery, La Technique, The Complete Guide to Modern Cooking, The Art of the Garde Mange and Patisserie interpreted nouvelle cuisine techniques and plated dishes. In 1977, the DIT began delivering courses in City & Guilds Advanced Kitchen & Larder 706/3 and Pastry 706/3, the only college in Ireland to do so at the time. Many graduates from these courses became the future Irish culinary lecturers, chef-proprietors, and culinary leaders. The next two decades saw a rise in fusion cooking, nouvelle cuisine, and a return to French classical cooking. Numerous Irish chefs were returning to Ireland having worked with Michelin starred chefs and opening new restaurants in the vein of classical French cooking, such as Kevin Thornton (Wine Epergne & Thorntons). These chefs were, in turn, influencing culinary training in DIT with a return to classical French cooking. New Classical French culinary textbooks such as New Classical Cuisine, The Modern Patisserie, The French Professional Pastry Series and Advanced Practical Cookery were being used in DIT In the last 15 years, science in cooking has become the current trend in culinary education in DIT. This is acknowledged by the increased number of culinary science textbooks and modules in molecular gastronomy offered in DIT. This also coincided with the launch of the BA (Hons) in Culinary Arts in DIT moving culinary education from a technical to a liberal education. Books such as The Science of Cooking, On Food and Cooking, The Fat Duck Cookbook and Modern Gastronomy now appear on recommended textbooks for culinary students.For the purpose of this article, practical classes held at DIT will be broken down as follows: hot kitchen class, larder classes, and pastry classes. These classes had recommended textbooks for each area. These can be broken down into three sections: hot kitche, larder, and pastry. This table identifies that the textbooks used in culinary education at DIT reflected the trends in cookery at the time they were being used. Hot Kitchen Larder Pastry Le Guide Culinaire. 1921. Le Guide Culinaire. 1921. The International Confectioner. 1968. Le Repertoire De La Cuisine. 1914. The Larder Chef, Classical Food Preparation and Presentation. 1969. Patisserie. 1971. All in the Cooking, Books 1&2. 1943 The Art of the Garde Manger. 1973. The Modern Patissier. 1986 Larousse Gastronomique. 1961. New Classic Cuisine. 1989. Professional French Pastry Series. 1987. Practical Cookery. 1962. The Curious Cook. 1990. Complete Pastrywork Techniques. 1991. Practical Professional Cookery. 1972. On Food and Cooking. The Science and Lore of the Kitchen. 1991. On Food and Cooking: The Science and Lore of the Kitchen. 1991 La Technique. 1976. Advanced Practical Cookery. 1995. Desserts: A Lifelong Passion. 1994. Escoffier: The Complete Guide to the Art of Modern Cookery. 1979. The Science of Cooking. 2000. Culinary Artistry. Dornenburg, 1996. Professional Cookery: The Process Approach. 1985. Garde Manger, The Art and Craft of the Cold Kitchen. 2004. Grande Finales: The Art of the Plated Dessert. 1997. On Food and Cooking: The Science and Lore of the Kitchen. 1991. The Science of Cooking. 2000. Fat Duck Cookbook. 2009. Modern Gastronomy. 2010. Tab.1. DIT Culinary Textbooks.1942–1960 During the first half of the 20th century, senior staff working in Dublin hotels, restaurants and clubs were predominately foreign born and trained. The two decades following World War II could be viewed as the “golden age” of haute cuisine in Dublin as many award-wining restaurants traded in the city at this time (Mac Con Iomaire “The Emergence”). Culinary education in DIT in 1942 saw the use of Escoffier’s Le Guide Culinaire as the defining textbook (Bowe). This was first published in 1903 and translated into English in 1907. In 1979 Cracknell and Kaufmann published a more comprehensive and update edited version under the title The Complete Guide to the Art of Modern Cookery by Escoffier for use in culinary colleges. This demonstrated that Escoffier’s work had withstood the test of the decades and was still relevant. Le Repertoire de La Cuisine by Louis Saulnier, a student of Escoffier, presented the fundamentals of French classical cookery. Le Repertoire was inspired by the work of Escoffier and contains thousands of classical recipes presented in a brief format that can be clearly understood by chefs and cooks. Le Repertoire remains an important part of any DIT culinary student’s textbook list. All in the Cooking by Josephine Marnell, Nora Breathnach, Ann Mairtin and Mor Murnaghan (1946) was one of the first cookbooks to be published in Ireland (Cashmann). This book was a domestic science cooking book written by lecturers in the Cathal Brugha Street College. There is a combination of classical French recipes and Irish recipes throughout the book. 1960s It was not until the 1960s that reference book Larousse Gastronomique and new textbooks such as Practical Cookery, The Larder Chef and International Confectionary made their way into DIT culinary education. These books still focused on classical French cooking but used lighter sauces and reflected more modern cooking equipment and techniques. Also, this period was the first time that specific books for larder and pastry work were introduced into the DIT culinary education system (Bowe). Larousse Gastronomique, which used Le Guide Culinaire as a basis (James), was first published in 1938 and translated into English in 1961. Practical Cookery, which is still used in DIT culinary education, is now in its 12th edition. Each edition has built on the previous, however, there is now criticism that some of the content is dated (Richards). Practical Cookery has established itself as a key textbook in culinary education both in Ireland and England. Practical Cookery recipes were laid out in easy to follow steps and food commodities were discussed briefly. The Larder Chef was first published in 1969 and is currently in its 4th edition. This book focuses on classical French larder techniques, butchery and fishmongery but recognises current trends and fashions in food presentation. The International Confectioner is no longer in print but is still used as a reference for basic recipes in pastry classes (Campbell). The Modern Patissier demonstrated more updated techniques and methods than were used in The International Confectioner. The Modern Patissier is still used as a reference book in DIT. 1970s The 1970s saw the decline in haute cuisine in Ireland, as it was in the process of being replaced by nouvelle cuisine. Irish chefs were being influenced by the works of chefs such as Paul Boucuse, Roger Verge, Michel Guerard, Raymond Olivier, Jean & Pierre Troisgros, Alain Senderens, Jacques Maniere, Jean Delaveine and Michel Guerard who advanced the uncomplicated natural presentation in food. Henri Gault claims that it was his manifesto published in October 1973 in Gault-Millau magazine which unleashed the movement called La Nouvelle Cuisine Française (Gault). In nouvelle cuisine, dishes in Carème and Escoffier’s style were rejected as over-rich and complicated. The principles underpinning this new movement focused on the freshness of ingredients, and lightness and harmony in all components and accompaniments, as well as basic and simple cooking methods and types of presentation. This was not, however, a complete overthrowing of the past, but a moving forward in the long-term process of cuisine development, utilising the very best from each evolution (Cousins). Books such as Practical Professional Cookery, The Art of the Garde Manger and Patisserie reflected this new lighter approach to cookery. Patisserie was first published in 1971, is now in its second edition, and continues to be used in DIT culinary education. This book became an essential textbook in pastrywork, and covers the entire syllabus of City & Guilds and CERT (now Fáilte Ireland). Patisserie covered all basic pastry recipes and techniques, while the second edition (in 1993) included new modern recipes, modern pastry equipment, commodities, and food hygiene regulations reflecting the changing catering environment. The Art of the Garde Manger is an American book highlighting the artistry, creativity, and cooking sensitivity need to be a successful Garde Manger (the larder chef who prepares cold preparation in a partie system kitchen). It reflected the dynamic changes occurring in the culinary world but recognised the importance of understanding basic French culinary principles. It is no longer used in DIT culinary education. La Technique is a guide to classical French preparation (Escoffier’s methods and techniques) using detailed pictures and notes. This book remains a very useful guide and reference for culinary students. Practical Professional Cookery also became an important textbook as it was written with the student and chef/lecturer in mind, as it provides a wider range of recipes and detailed information to assist in understanding the tasks at hand. It is based on classical French cooking and compliments Practical Cookery as a textbook, however, its recipes are for ten portions as opposed to four portions in Practical Cookery. Again this book was written with the City & Guilds examinations in mind. 1980s During the mid-1980s, many young Irish chefs and waiters emigrated. They returned in the late-1980s and early-1990s having gained vast experience of nouvelle and fusion cuisine in London, Paris, New York, California and elsewhere (Mac Con Iomaire, “The Changing”). These energetic, well-trained professionals began opening chef-proprietor restaurants around Dublin, providing invaluable training and positions for up-and-coming young chefs, waiters and culinary college graduates. The 1980s saw a return to French classical cookery textbook such as Professional Cookery: The Process Approach, New Classic Cuisine and the Professional French Pastry series, because educators saw the need for students to learn the basics of French cookery. Professional Cookery: The Process Approach was written by Daniel Stevenson who was, at the time, a senior lecturer in Food and Beverage Operations at Oxford Polytechnic in England. Again, this book was written for students with an emphasis on the cookery techniques and the practices of professional cookery. The Complete Guide to Modern Cooking by Escoffier continued to be used. This book is used by cooks and chefs as a reference for ingredients in dishes rather than a recipe book, as it does not go into detail in the methods as it is assumed the cook/chef would have the required experience to know the method of production. Le Guide Culinaire was only used on advanced City & Guilds courses in DIT during this decade (Bowe). New Classic Cuisine by the classically French trained chefs, Albert and Michel Roux (Gayot), is a classical French cuisine cookbook used as a reference by DIT culinary educators at the time because of the influence the Roux brothers were having over the English fine dining scene. The Professional French Pastry Series is a range of four volumes of pastry books: Vol. 1 Doughs, Batters and Meringues; Vol. 2 Creams, Confections and Finished Desserts; Vol. 3 Petit Four, Chocolate, Frozen Desserts and Sugar Work; and Vol. 4 Decorations, Borders and Letters, Marzipan, Modern Desserts. These books about classical French pastry making were used on the advanced pastry courses at DIT as learners needed a basic knowledge of pastry making to use them. 1990s Ireland in the late 1990s became a very prosperous and thriving European nation; the phenomena that became known as the “celtic tiger” was in full swing (Mac Con Iomaire “The Changing”). The Irish dining public were being treated to a resurgence of traditional Irish cuisine using fresh wholesome food (Hughes). The Irish population was considered more well-educated and well travelled than previous generations and culinary students were now becoming interested in the science of cooking. In 1996, the BA (Hons) in Culinary Arts program at DIT was first mooted (Hegarty). Finally, in 1999, a primary degree in Culinary Arts was sanctioned by the Department of Education underpinned by a new liberal/vocational philosophy in education (Duff). Teaching culinary arts in the past had been through a vocational education focus whereby students were taught skills for industry which were narrow, restrictive, and constraining, without the necessary knowledge to articulate the acquired skill. The reading list for culinary students reflected this new liberal education in culinary arts as Harold McGee’s books The Curious Cook and On Food and Cooking: The Science and Lore of the Kitchen explored and explained the science of cooking. On Food and Cooking: The Science and Lore of the Kitchen proposed that “science can make cooking more interesting by connecting it with the basic workings of the natural world” (Vega 373). Advanced Practical Cookery was written for City & Guilds students. In DIT this book was used by advanced culinary students sitting Fáilte Ireland examinations, and the second year of the new BA (Hons) in Culinary Arts. Culinary Artistry encouraged chefs to explore the creative process of culinary composition as it explored the intersection of food, imagination, and taste (Dornenburg). This book encouraged chefs to develop their own style of cuisine using fresh seasonal ingredients, and was used for advanced students but is no longer a set text. Chefs were being encouraged to show their artistic traits, and none more so than pastry chefs. Grande Finale: The Art of Plated Desserts encouraged advanced students to identify different “schools” of pastry in relation to the world of art and design. The concept of the recipes used in this book were built on the original spectacular pieces montées created by Antoine Carême. 2000–2013 After nouvelle cuisine, recent developments have included interest in various fusion cuisines, such as Asia-Pacific, and in molecular gastronomy. Molecular gastronomists strive to find perfect recipes using scientific methods of investigation (Blanck). Hervè This experimentation with recipes and his introduction to Nicholos Kurti led them to create a food discipline they called “molecular gastronomy”. In 1998, a number of creative chefs began experimenting with the incorporation of ingredients and techniques normally used in mass food production in order to arrive at previously unattainable culinary creations. This “new cooking” (Vega 373) required a knowledge of chemical reactions and physico-chemical phenomena in relation to food, as well as specialist tools, which were created by these early explorers. It has been suggested that molecular gastronomy is “science-based cooking” (Vega 375) and that this concept refers to conscious application of the principles and tools from food science and other disciplines for the development of new dishes particularly in the context of classical cuisine (Vega). The Science of Cooking assists students in understanding the chemistry and physics of cooking. This book takes traditional French techniques and recipes and refutes some of the claims and methods used in traditional recipes. Garde Manger: The Art and Craft of the Cold Kitchen is used for the advanced larder modules at DIT. This book builds on basic skills in the Larder Chef book. Molecular gastronomy as a subject area was developed in 2009 in DIT, the first of its kind in Ireland. The Fat Duck Cookbook and Modern Gastronomy underpin the theoretical aspects of the module. This module is taught to 4th year BA (Hons) in Culinary Arts students who already have three years experience in culinary education and the culinary industry, and also to MSc Culinary Innovation and Food Product Development students. Conclusion Escoffier, the master of French classical cuisine, still influences culinary textbooks to this day. His basic approach to cooking is considered essential to teaching culinary students, allowing them to embrace the core skills and competencies required to work in the professional environment. Teaching of culinary arts at DIT has moved vocational education to a more liberal basis, and it is imperative that the chosen textbooks reflect this development. This liberal education gives the students a broader understanding of cooking, hospitality management, food science, gastronomy, health and safety, oenology, and food product development. To date there is no practical culinary textbook written specifically for Irish culinary education, particularly within this new liberal/vocational paradigm. There is clearly a need for a new textbook which combines the best of Escoffier’s classical French techniques with the more modern molecular gastronomy techniques popularised by Ferran Adria. References Adria, Ferran. Modern Gastronomy A to Z: A Scientific and Gastronomic Lexicon. London: CRC P, 2010. Barker, William. The Modern Patissier. London: Hutchinson, 1974. Barham, Peter. The Science of Cooking. Berlin: Springer-Verlag, 2000. Bilheux, Roland, Alain Escoffier, Daniel Herve, and Jean-Maire Pouradier. Special and Decorative Breads. New York: Van Nostrand Reinhold, 1987. Blanck, J. "Molecular Gastronomy: Overview of a Controversial Food Science Discipline." Journal of Agricultural and Food Information 8.3 (2007): 77-85. Blumenthal, Heston. The Fat Duck Cookbook. London: Bloomsbury, 2001. Bode, Willi, and M.J. Leto. The Larder Chef. Oxford: Butter-Heinemann, 1969. Bowe, James. Personal Communication with Author. Dublin. 7 Apr. 2013. Boyle, Tish, and Timothy Moriarty. Grand Finales, The Art of the Plated Dessert. New York: John Wiley, 1997. Campbell, Anthony. Personal Communication with Author. Dublin, 10 Apr. 2013. Cashman, Dorothy. "An Exploratory Study of Irish Cookbooks." Unpublished M.Sc Thesis. Dublin: Dublin Institute of Technology, 2009. Ceserani, Victor, Ronald Kinton, and David Foskett. Practical Cookery. London: Hodder & Stoughton Educational, 1962. Ceserani, Victor, and David Foskett. Advanced Practical Cookery. London: Hodder & Stoughton Educational, 1995. Corr, Frank. Hotels in Ireland. Dublin: Jemma, 1987. Cousins, John, Kevin Gorman, and Marc Stierand. "Molecular Gastronomy: Cuisine Innovation or Modern Day Alchemy?" International Journal of Hospitality Management 22.3 (2009): 399–415. Cracknell, Harry Louis, and Ronald Kaufmann. Practical Professional Cookery. London: MacMillan, 1972. Cracknell, Harry Louis, and Ronald Kaufmann. Escoffier: The Complete Guide to the Art of Modern Cookery. New York: John Wiley, 1979. Dornenburg, Andrew, and Karen Page. Culinary Artistry. New York: John Wiley, 1996. Duff, Tom, Joseph Hegarty, and Matt Hussey. The Story of the Dublin Institute of Technology. Dublin: Blackhall, 2000. Escoffier, Auguste. Le Guide Culinaire. France: Flammarion, 1921. Escoffier, Auguste. The Complete Guide to the Art of Modern Cookery. Ed. Crachnell, Harry, and Ronald Kaufmann. New York: John Wiley, 1986. Gault, Henri. Nouvelle Cuisine, Cooks and Other People: Proceedings of the Oxford Symposium on Food and Cookery 1995. Devon: Prospect, 1996. 123-7. Gayot, Andre, and Mary, Evans. "The Best of London." Gault Millau (1996): 379. Gillespie, Cailein. "Gastrosophy and Nouvelle Cuisine: Entrepreneurial Fashion and Fiction." British Food Journal 96.10 (1994): 19-23. Gisslen, Wayne. Professional Cooking. Hoboken: John Wiley, 2011. Hanneman, Leonard. Patisserie. Oxford: Butterworth-Heinemann, 1971. Hegarty, Joseph. Standing the Heat. New York: Haworth P, 2004. Hsu, Kathy. "Global Tourism Higher Education Past, Present and Future." Journal of Teaching in Travel and Tourism 5.1/2/3 (2006): 251-267 Hughes, Mairtin. Ireland. Victoria: Lonely Planet, 2000. Ireland. Irish Statute Book: Dublin Institute of Technology Act 1992. Dublin: Stationery Office, 1992. James, Ken. Escoffier: The King of Chefs. Hambledon: Cambridge UP, 2002. Lawson, John, and Harold, Silver. Social History of Education in England. London: Methuen, 1973. Lehmann, Gilly. "English Cookery Books in the 18th Century." The Oxford Companion to Food. Oxford: Oxford UP, 1999. 227-9. Marnell, Josephine, Nora Breathnach, Ann Martin, and Mor Murnaghan. All in the Cooking Book 1 & 2. Dublin: Educational Company of Ireland, 1946. Mac Con Iomaire, Máirtín. "The Changing Geography and Fortunes of Dublin's Haute Cuisine Restaurants, 1958-2008." Food, Culture and Society: An International Journal of Multidisiplinary Research 14.4 (2011): 525-45. ---. "Chef Liam Kavanagh (1926-2011)." Gastronomica: The Journal of Food and Culture 12.2 (2012): 4-6. ---. "The Emergence, Development and Influence of French Haute Cuisine on Public Dining in Dublin Restaurants 1900-2000: An Oral History". PhD. Thesis. Dublin: Dublin Institute of Technology, 2009. McGee, Harold. The Curious Cook: More Kitchen Science and Lore. New York: Hungry Minds, 1990. ---. On Food and Cooking the Science and Lore of the Kitchen. London: Harper Collins, 1991. Montague, Prosper. Larousse Gastronomique. New York: Crown, 1961. National Qualification Authority of Ireland. "Review by the National Qualifications Authority of Ireland (NQAI) of the Effectiveness of the Quality Assurance Procedures of the Dublin Institute of Technology." 2010. 18 Feb. 2012 ‹http://www.dit.ie/media/documents/services/qualityassurance/terms_of_ref.doc› Nicolello, Ildo. Complete Pastrywork Techniques. London: Hodder & Stoughton, 1991. Pepin, Jacques. La Technique. New York: Black Dog & Leventhal, 1976. Richards, Peter. "Practical Cookery." 9th Ed. Caterer and Hotelkeeper (2001). 18 Feb. 2012 ‹http://www.catererandhotelkeeper.co.uk/Articles/30/7/2001/31923/practical-cookery-ninth-edition-victor-ceserani-ronald-kinton-and-david-foskett.htm›. Roux, Albert, and Michel Roux. New Classic Cuisine. New York: Little, Brown, 1989. Roux, Michel. Desserts: A Lifelong Passion. London: Conran Octopus, 1994. Saulnier, Louis. Le Repertoire De La Cuisine. London: Leon Jaeggi, 1914. Sonnenschmidt, Fredric, and John Nicholas. The Art of the Garde Manger. New York: Van Nostrand Reinhold, 1973. Spang, Rebecca. The Invention of the Restaurant: Paris and Modern Gastronomic Culture. Cambridge: Harvard UP, 2000. Stevenson, Daniel. Professional Cookery the Process Approach. London: Hutchinson, 1985. The Culinary Institute of America. Garde Manger: The Art and Craft of the Cold Kitchen. Hoboken: New Jersey, 2004. Vega, Cesar, and Job, Ubbink. "Molecular Gastronomy: A Food Fad or Science Supporting Innovation Cuisine?". Trends in Food Science & Technology 19 (2008): 372-82. Wilfred, Fance, and Michael Small. The New International Confectioner: Confectionary, Cakes, Pastries, Desserts, Ices and Savouries. 1968.