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1

Kennedy, Jessica R., Christopher D. G. Harley, and Katie E. Marshall. "Drivers of plasticity in freeze tolerance in the intertidal mussel Mytilus trossulus." Journal of Experimental Biology 223, no. 24 (2020): jeb233478. http://dx.doi.org/10.1242/jeb.233478.

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ABSTRACTFreezing is an extreme stress to living cells, and so freeze-tolerant animals often accumulate protective molecules (termed cryoprotectants) to prevent the cellular damage caused by freezing. The bay mussel, Mytilus trossulus, is an ecologically important intertidal invertebrate that can survive freezing. Although much is known about the biochemical correlates of freeze tolerance in insects and vertebrates, the cryoprotectants that are used by intertidal invertebrates are not well characterized. Previous work has proposed two possible groups of low-molecular weight cryoprotectants in i
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Anderson, Jeff, Charles Taliaferro, and Dennis Martin. "Freeze tolerance of bermudagrasses." Crop Science 42, no. 3 (2002): 975. http://dx.doi.org/10.2135/cropsci2002.0975.

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Anderson, Jeff, Charles Taliaferro, and Dennis Martin. "Freeze Tolerance of bermudagrasses." Crop Science 42, no. 3 (2002): 975–77. http://dx.doi.org/10.2135/cropsci2002.9750.

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4

Storey, K. B., and J. M. Storey. "Freeze tolerance in animals." Physiological Reviews 68, no. 1 (1988): 27–84. http://dx.doi.org/10.1152/physrev.1988.68.1.27.

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5

Chu, Ye, Josh Clevenger, Kendall Lee, Jing Zhang, and Changying Li. "Genetic Breeding to Improve Freeze Tolerance in Blueberries, a Review." Horticulturae 11, no. 6 (2025): 614. https://doi.org/10.3390/horticulturae11060614.

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The abiotic stresses associated with spring/fall freezes and extreme winter cold cause significant economic losses in blueberry production. These problems are exacerbated by climate change and increasingly erratic weather patterns. Developing freeze-tolerant blueberry cultivars with optimized cold hardiness, chilling requirement, and flowering and fruiting phenology holds promise for mitigating the risk of these weather-related damages. These weather-resilient cultivars will ensure the long-term productivity and sustainability of the blueberry industry. The focus of this review is to present t
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6

Tanghe, An, Patrick Van Dijck, Françoise Dumortier, Aloys Teunissen, Stefan Hohmann, and Johan M. Thevelein. "Aquaporin Expression Correlates with Freeze Tolerance in Baker's Yeast, and Overexpression Improves Freeze Tolerance in Industrial Strains." Applied and Environmental Microbiology 68, no. 12 (2002): 5981–89. http://dx.doi.org/10.1128/aem.68.12.5981-5989.2002.

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ABSTRACT Little information is available about the precise mechanisms and determinants of freeze resistance in baker's yeast, Saccharomyces cerevisiae. Genomewide gene expression analysis and Northern analysis of different freeze-resistant and freeze-sensitive strains have now revealed a correlation between freeze resistance and the aquaporin genes AQY1 and AQY2. Deletion of these genes in a laboratory strain rendered yeast cells more sensitive to freezing, while overexpression of the respective genes, as well as heterologous expression of the human aquaporin gene hAQP1, improved freeze tolera
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7

Toxopeus, Jantina, Vladimír Koštál, and Brent J. Sinclair. "Evidence for non-colligative function of small cryoprotectants in a freeze-tolerant insect." Proceedings of the Royal Society B: Biological Sciences 286, no. 1899 (2019): 20190050. http://dx.doi.org/10.1098/rspb.2019.0050.

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Freeze tolerance, the ability to survive internal ice formation, facilitates survival of some insects in cold habitats. Low-molecular-weight cryoprotectants such as sugars, polyols and amino acids are hypothesized to facilitate freeze tolerance, but their in vivo function is poorly understood. Here, we use a combination of metabolomics and manipulative experiments in vivo and ex vivo to examine the function of multiple cryoprotectants in the spring field cricket Gryllus veletis . Cold-acclimated G. veletis are freeze-tolerant and accumulate myo -inositol, proline and trehalose in their haemoly
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8

Caglar, Ufuk, John Mark Lawton, Juan Carlos Melgar, and Ksenija Gasic. "Fruitlet Freeze Tolerance in Peach Germplasm." Agronomy 14, no. 2 (2024): 302. http://dx.doi.org/10.3390/agronomy14020302.

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Climate change is affecting the production of temperate fruit crops. Freeze damage, particularly in spring, has resulted in significant economic losses in peach production in the southeastern United States. Research efforts in peach and other Prunus species have primarily focused on dormancy-related traits associated with bloom time, such as chill and heat requirement, with fruitlet freeze tolerance not equally represented. This study reports fruitlet freeze tolerance in 75 peach and nectarine accessions at six freezing temperatures (0 to −10 °C) using electrolyte leakage method over two seaso
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9

Dinkelacker, S. A., J. P. Costanzo, and R. E. Lee. "Anoxia tolerance and freeze tolerance in hatchling turtles." Journal of Comparative Physiology B 175, no. 3 (2005): 209–17. http://dx.doi.org/10.1007/s00360-005-0478-0.

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10

Anderson, J. A., and Y. Q. Wu. "Freeze tolerance of forage bermudagrasses." Grass and Forage Science 66, no. 3 (2011): 449–52. http://dx.doi.org/10.1111/j.1365-2494.2011.00802.x.

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11

Overgaard, J. "WATER CHANNELS DRIVE FREEZE TOLERANCE." Journal of Experimental Biology 209, no. 15 (2006): v—vi. http://dx.doi.org/10.1242/jeb.02408.

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12

Costanzo, J. P., D. L. Claussen, and R. E. Lee. "Freeze tolerance in a reptile." Cryobiology 25, no. 6 (1988): 519. http://dx.doi.org/10.1016/0011-2240(88)90329-x.

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13

Toxopeus, Jantina, and Brent J. Sinclair. "Mechanisms underlying insect freeze tolerance." Biological Reviews 93, no. 4 (2018): 1891–914. http://dx.doi.org/10.1111/brv.12425.

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14

Storey, KB. "What Contributes to Freeze Tolerance?" Physiology 2, no. 5 (1987): 157–60. http://dx.doi.org/10.1152/physiologyonline.1987.2.5.157.

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Unique among vertebrates, some frogs withstand freezing of the whole body during overwintering. How can they tolerate complete stoppage of breathing, heartbeat, and blood flow when the animal freezes, to start again on thawing? They provide a fascinating view of life in a frozen state and offer a model system for possible medical use in organ cryopreservation.
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15

Manley, Reeser C., and Rita L. Hummel. "Mefluidide Does Not Consistently Enhance the Freezing Tolerance of Cabbage." HortScience 31, no. 3 (1996): 402–4. http://dx.doi.org/10.21273/hortsci.31.3.402.

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Mefluidide, a synthetic plant growth regulator, has been reported to protect chilling-sensitive plants from chilling damage and enhance the freezing tolerance of certain winter-hardy herbaceous plants. The potential of mefluidide to enhance the freezing tolerance of nonhardened and dehardening cabbage (Brassica oleracea L. Capitata Group) leaf tissue was investigated. Mefluidide at 0 to 60 mg·L–1 was tested on `Brunswick' and `Golden Acre' cabbage in five experiments. Leaf tissue freezing tolerance was measured 3 to 9 days postapplication by electrolyte leakage assay. The interval between appl
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16

Costanzo, J. P., R. E. Lee, and P. H. Lortz. "Physiological responses of freeze-tolerant and -intolerant frogs: clues to evolution of anuran freeze tolerance." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 265, no. 4 (1993): R721—R725. http://dx.doi.org/10.1152/ajpregu.1993.265.4.r721.

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Freeze tolerance in the wood frog, Rana sylvatica, is promoted by multiple, integrated physiological responses to ice forming within body tissues. By analyzing the freezing responses of the sympatric, but freeze intolerant, leopard frog (R. pipiens), we sought clues to the evolution of anuran freeze tolerance. Physiological responses critical to R. sylvatica's freeze tolerance, such as the synthesis and distribution of the cryoprotectant glucose, protective dehydration of organs, and deferred cardiac failure, were present, but comparatively less pronounced, in R. pipiens. Both species were inn
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17

McKellar, M. A., D. W. Buchanan, Dewayne L. Ingram, and C. W. Campbell. "Freezing Tolerance of Avocado Leaves." HortScience 27, no. 4 (1992): 341–43. http://dx.doi.org/10.21273/hortsci.27.4.341.

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Freezing tolerance and the lethal freezing temperature were determined for detached leaves of avocado (Persea americana Mill.) by either electrolyte leakage or visual appearance of browning. Leaves from field-grown trees of `Gainesville', `Booth8', and `Winter Mexican' in both Gainesville and Homestead, Fla., were evaluated. All cultivars in both locations survived ice formation in their tissue. Leaf tissue had a temperature limit (lethal freeze temperature) at and below which the tissue died. The lethal freezing temperature varied from -5.1 to -9.3C, depending on time of year and location. Th
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18

Storey, K. B. "Life in the freezer: molecular mechanisms of freeze tolerance in vertebrates." Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 126 (July 2000): 141. http://dx.doi.org/10.1016/s1095-6433(00)80280-9.

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19

Min, Kyungwon, Yunseo Cho, Eunjeong Kim, Minho Lee, and Sang-Ryong Lee. "Exogenous Glycine Betaine Application Improves Freezing Tolerance of Cabbage (Brassica oleracea L.) Leaves." Plants 10, no. 12 (2021): 2821. http://dx.doi.org/10.3390/plants10122821.

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Exogenous glycine betaine (GB) application has been reported to improve plant tolerance to various abiotic stresses, but its effect on freezing tolerance has not been well studied. We investigated the effect of exogenous GB on freezing tolerance of cabbage (Brassica oleracea L.) leaves. Seedlings fed with 30 mM GB via sub-irrigation showed effectively assimilated GB as evident by higher GB concentration. Exogenous GB did not retard leaf-growth (fresh weight, dry weight, and leaf area) rather slightly promoted it. Temperature controlled freeze-thaw tests proved GB-fed plants were more freeze-to
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20

Morgan-Richards, Mary, Craig J. Marshall, Patrick J. Biggs, and Steven A. Trewick. "Insect Freeze-Tolerance Downunder: The Microbial Connection." Insects 14, no. 1 (2023): 89. http://dx.doi.org/10.3390/insects14010089.

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Insects that are freeze-tolerant start freezing at high sub-zero temperatures and produce small ice crystals. They do this using ice-nucleating agents that facilitate intercellular ice growth and prevent formation of large crystals where they can damage tissues. In Aotearoa/New Zealand the majority of cold adapted invertebrates studied survive freezing at any time of year, with ice formation beginning in the rich microbiome of the gut. Some freeze-tolerant insects are known to host symbiotic bacteria and/or fungi that produce ice-nucleating agents and we speculate that gut microbes of many New
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21

Qi-bin Hong, Xi-jun Ma, Gui-zhi Gong, Zhu-chun Peng, and Yong-rui He. "QTL MAPPING OF CITRUS FREEZE TOLERANCE." Acta Horticulturae, no. 1065 (January 2015): 467–74. http://dx.doi.org/10.17660/actahortic.2015.1065.57.

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22

Ketchie, D. O., and R. Kammereck. "Freeze Tolerance of `Braeburn' Apple Shoots." HortScience 30, no. 4 (1995): 775A—775. http://dx.doi.org/10.21273/hortsci.30.4.775a.

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Differential thermal analysis (DTA) and tetrazolium triphenyl chloride (TTC) were done on shoots of 4-year-old `Braeburn' apple trees for 3 years. The trees acclimated slowly in autumn. If cold temperatures last long enough in winter, shoots will acclimate as low as –40C. Shoots are sensitive to warm temperatures and deacclimated rapidly. An attempt to run a controlled test on freeze resistance of `Braeburn' did not respond to DTA. Moisture samples indicated trees were freeze dried. Different sets of trees were rehydrated and showed an exotherm pattern. Exotherms could be seen after 3 days at
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23

Storey, Kenneth B., and Janet M. Storey. "Freeze tolerance: constraining forces, adaptive mechanisms." Canadian Journal of Zoology 66, no. 5 (1988): 1122–27. http://dx.doi.org/10.1139/z88-164.

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For a variety of ectothermic animals, survival of subzero temperatures is aided by a natural capacity to tolerate extracellular freezing. Both low temperatures and freezing place inescapable constraints on the behaviour of molecules and biological structures. For example, low temperature affects metabolic rates, membrane fluidity, and weak bond interactions governing protein structure and function, whereas damage from freezing includes osmotic stress, membrane deformation, dehydration, physical damage by ice, and the consequences of long-term ischaemia. Selected biochemical adaptations permit
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24

Storey, K. B., and J. M. Storey. "Natural Freeze Tolerance in Ectothermic Vertebrates." Annual Review of Physiology 54, no. 1 (1992): 619–37. http://dx.doi.org/10.1146/annurev.ph.54.030192.003155.

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25

Des Marteaux, Lauren E., Petr Hůla, and Vladimír Koštál. "Transcriptional analysis of insect extreme freeze tolerance." Proceedings of the Royal Society B: Biological Sciences 286, no. 1913 (2019): 20192019. http://dx.doi.org/10.1098/rspb.2019.2019.

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Few invertebrates can survive cryopreservation in liquid nitrogen, and the mechanisms by which some species do survive are underexplored, despite high application potential. Here, we turn to the drosophilid Chymomyza costata to strengthen our fundamental understanding of extreme freeze tolerance and gain insights about potential avenues for cryopreservation of biological materials. We first use RNAseq to generate transcriptomes of three C. costata larval phenotypic variants: those warm-acclimated in early or late diapause (weak capacity to survive cryopreservation), and those undergoing cold a
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26

Storey, Kenneth B. "Biochemistry of natural freeze tolerance in animals: molecular adaptations and applications to cryopreservation." Biochemistry and Cell Biology 68, no. 4 (1990): 687–98. http://dx.doi.org/10.1139/o90-100.

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For a wide variety of animals, winter survival in cold climates includes the ability to tolerate ice formation in extracellular body fluids. Among terrestrially hibernating vertebrates, freeze tolerance has been documented for five amphibian and two reptile species. These species may survive for days or weeks in a frozen state with no breathing and no heart beat, and with up to 65% of total body water as extracellular ice. The biochemical mechanisms involved in natural freeze tolerance include (i) the regulation of extracellular ice formation by proteinaceous ice nucleators in body fluids, (ii
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27

Teunissen, Aloys, Françoise Dumortier, Marie-Françoise Gorwa, et al. "Isolation and Characterization of a Freeze-Tolerant Diploid Derivative of an Industrial Baker's Yeast Strain and Its Use in Frozen Doughs." Applied and Environmental Microbiology 68, no. 10 (2002): 4780–87. http://dx.doi.org/10.1128/aem.68.10.4780-4787.2002.

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ABSTRACT The routine production and storage of frozen doughs are still problematic. Although commercial baker's yeast is highly resistant to environmental stress conditions, it rapidly loses stress resistance during dough preparation due to the initiation of fermentation. As a result, the yeast loses gassing power significantly during storage of frozen doughs. We obtained freeze-tolerant mutants of polyploid industrial strains following screening for survival in doughs prepared with UV-mutagenized yeast and subjected to 200 freeze-thaw cycles. Two strains in the S47 background with a normal gr
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28

Costanzo, JP, and RE, Lee. "Biophysical and Physiological Responses promoting Freeze Tolerance in Vertebrates." Physiology 9, no. 6 (1994): 252–56. http://dx.doi.org/10.1152/physiologyonline.1994.9.6.252.

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Freeze tolerance, an overwintering adaptation of at least 10 species of ectothermic vertebrates, is promoted by integrated biophysical and physiological responses to ice forming within tissues. Application of physiological principles of natural freeze tolerance has accelerated the development of protocols for cryopreserving mammalian organs.
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Zhang, Can-Kui, Ping Lang, Robert C. Ebel, et al. "Down-regulated gene expression of cold acclimated Poncirus trifoliata." Canadian Journal of Plant Science 85, no. 2 (2005): 417–24. http://dx.doi.org/10.4141/p04-130.

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Citrus sp. are important commercial fruit crops throughout the world that are occasionally devastated by subfreezing temperatures. Poncirus trifoliata (maximum freeze tolerance of -26°C) is a close relative of commercial Citrus sp. (maximum freeze tolerance of -10°C) that has been used in breeding programs to develop more cold-hardy genotypes and as a rootstock to enhance freeze tolerance of the scion. Species with greater freeze tolerance vary in gene expression during cold acclimating temperatures. mRNA differential display (DDRT-PCR) and quantitative relative RT-PCR were used to study down
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30

Anderson, Jeff A. "Does FreezePruf Topical Spray Increase Plant Resistance to Freezing Stress?" HortTechnology 22, no. 4 (2012): 542–46. http://dx.doi.org/10.21273/horttech.22.4.542.

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One method of plant freeze protection involves the application of compounds that promote freeze avoidance or tolerance. FreezePruf, a commercially available product recently marketed to improve both freeze avoidance and tolerance, contains polyethylene glycol, potassium silicate, glycerol, silicone polyether surfactant, and a bicyclic oxazolidine antidessicant. The goal of the present study was to evaluate the protection level provided by FreezePruf using laboratory-based methods involving plants and plant parts from species capable and incapable of low-temperature acclimation. FreezePruf did
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31

Storey, Kenneth B., and Janet M. Storey. "Molecular Physiology of Freeze Tolerance in Vertebrates." Physiological Reviews 97, no. 2 (2017): 623–65. http://dx.doi.org/10.1152/physrev.00016.2016.

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Freeze tolerance is an amazing winter survival strategy used by various amphibians and reptiles living in seasonally cold environments. These animals may spend weeks or months with up to ∼65% of their total body water frozen as extracellular ice and no physiological vital signs, and yet after thawing they return to normal life within a few hours. Two main principles of animal freeze tolerance have received much attention: the production of high concentrations of organic osmolytes (glucose, glycerol, urea among amphibians) that protect the intracellular environment, and the control of ice withi
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32

Yelenosky, G., and J. C. V. Vu. "Ability of `Valencia' Sweet Orange to Cold-acclimate on Cold-sensitive Citron Rootstock." HortScience 27, no. 11 (1992): 1201–3. http://dx.doi.org/10.21273/hortsci.27.11.1201.

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Greenhouse-grown l-year-old sweet orange trees [Citrus sinensis (L.) Osbeck cv. Valencia] on cold-hardy trifoliate orange [Poncirus trifoliata (L.) Raf.] and cold-sensitive citron (C. medica L.) rootstocks were exposed to cold-acclimation conditions and freeze-tested at -6.7C for 4 hours in a temperature-programed walk-in freezer room. Nonhardened trees generally did not survive the freeze, whereas cold-hardened trees survived with no wood kill on either rootstock. Essentially, all leaves died or abscised during the subsequent 5 weeks in the greenhouse. Freeze survival did not separate rootsto
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33

Lee, Yechan, Hyojung Choi, and Sang Sook Lee. "Identification and Utilization of Cold Stress Responsive ERF Genes in Overwintering Chinese Cabbage." Korean Science Education Society for the Gifted 16, no. 1 (2024): 118–30. http://dx.doi.org/10.29306/jseg.2024.16.1.118.

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To mitigate plant freeze damage caused by extreme weather events, we need to elucidate the freeze resistance mechanism of freeze-tolerant plants through molecular biological approaches. Our research focuses on identifying the cold stress responsive, ERF(Ethylene-Responsive transcription Factor) genes that confer freeze tolerance in overwintering Chinese cabbage. Additionally, we aim to explore the molecular basis for conferring freezing tolerance to other organisms through genetic transformation. Expression of the ERF, cold stress responsive gene in overwintering Chinese cabbage, leads to the
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Lu, Yuzhen, Kitt G. Payn, Piyush Pandey, et al. "Hyperspectral Imaging with Cost-Sensitive Learning for High-Throughput Screening of Loblolly Pine (Pinus taeda L.) Seedlings for Freeze Tolerance." Transactions of the ASABE 64, no. 6 (2021): 2045–59. http://dx.doi.org/10.13031/trans.14708.

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HighlightsA hyperspectral imaging approach was developed for freeze-tolerance phenotyping of loblolly pine seedlings.Image acquisition was conducted before and periodically after artificial freezing of the seedlings.A hyperspectral data processing pipeline was developed to extract the spectra from seedling segments.Cost-sensitive support vector machine (SVM) was used for classifying stressed and healthy seedlings.Post-freeze scanning of seedlings on day 41 achieved the highest screening accuracy of 97%.Abstract. Loblolly pine (Pinus taeda L.) is a commercially important timber species planted
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35

Costanzo, J. P., and R. E. Lee. "Freeze-thaw injury in erythrocytes of the freeze-tolerant wood frog, Rana sylvatica." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 261, no. 6 (1991): R1346—R1350. http://dx.doi.org/10.1152/ajpregu.1991.261.6.r1346.

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Erythrocytes from the freeze-tolerant wood frog (Rana sylvatica) were subjected to in vitro tests of freeze tolerance, cryoprotection, and osmotic fragility. The responses of cells from frogs acclimated to 4 or 15 degrees C were similar. Erythrocytes that were frozen in saline hemolyzed at -4 degrees C or lower. The addition of high concentrations (150 and 1,500 mM) of glucose or glycerol, cryoprotectants produced naturally by freeze-tolerant frogs, significantly reduced cell injury at -8 degrees C, but concentrations of 1.5 or 15 mM were ineffective. Hemolysis was reduced by 94% with 1,500 mM
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Xiang, Mingying, Shuhao Yu, Lakshmy Gopinath, Hassan Salahi, Justin Q. Moss, and Yanqi Wu. "Raising Mowing Height Improves Freeze Tolerance of Putting Green–type Bermudagrass." HortScience 58, no. 11 (2023): 1277–81. http://dx.doi.org/10.21273/hortsci17351-23.

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There is a growing trend of cultivating hybrid bermudagrass [Cynodon dactylon (L.) Pers. × Cynodon transvaalensis Burtt-Davy] on golf course putting greens in the transition zone because of its excellent quality in the summer months, coupled with less pesticide input than creeping bentgrass (Agrostis stolonifera L.). However, the long-term success of bermudagrass putting greens is hindered by low temperatures in winter months, particularly in the transition zone. To address this issue, in addition to genetic improvement for cold hardiness through the development of new cultivars, effective man
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37

Shima, Jun, Yuko Sakata-Tsuda, Yasuo Suzuki, et al. "Disruption of the CAR1 Gene Encoding Arginase Enhances Freeze Tolerance of the Commercial Baker's Yeast Saccharomyces cerevisiae." Applied and Environmental Microbiology 69, no. 1 (2003): 715–18. http://dx.doi.org/10.1128/aem.69.1.715-718.2003.

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ABSTRACT The effect of intracellular charged amino acids on freeze tolerance in doughs was determined by constructing homozygous diploid arginase-deficient mutants of commercial baker's yeast. An arginase mutant accumulated higher levels of arginine and/or glutamate and showed increased leavening ability during the frozen-dough baking process, suggesting that disruption of the CAR1 gene enhances freeze tolerance.
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38

ELHANAFI, D., B. LEENANON, W. BANG, and M. A. DRAKE. "Impact of Cold and Cold-Acid Stress on Poststress Tolerance and Virulence Factor Expression ofEscherichia coli O157:H7†‡." Journal of Food Protection 67, no. 1 (2004): 19–26. http://dx.doi.org/10.4315/0362-028x-67.1.19.

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The effect of extended cold or cold-acid storage ofEscherichia coli O157:H7 on subsequent acid tolerance, freeze-thaw survival, heat tolerance, and virulence factor (Shiga toxin, intimin, and hemolysin) expression was determined. ThreeE. coli O157:H7 strains were stressed at 4°C in TSB or pH 5.5 TSB for 4 weeks. The acid (TSB [pH 2.0] or simulated gastric fluid [pH 1.5]) tolerance, freeze-thaw (−20°C to 21°C) survival, and heat (56°C) tolerance of stressed cells were compared with those of control cells. The β-galactosidase activities of stressed and control cells containing a lacZ gene fusion
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LEENANON, B., and M. A. DRAKE. "Acid Stress, Starvation, and Cold Stress Affect Poststress Behavior of Escherichia coli O157:H7 and Nonpathogenic Escherichia coli†." Journal of Food Protection 64, no. 7 (2001): 970–74. http://dx.doi.org/10.4315/0362-028x-64.7.970.

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The effects of acid shock, acid adaptation, starvation, and cold stress of Escherichia coli O157:H7 (ATCC 43895), an rpo S mutant (FRIK 816-3), and nonpathogenic E. coli (ATCC 25922) on poststress heat resistance and freeze–thaw resistance were investigated. Following stress, heat tolerance at 56°C and freeze–thaw resistance at −20 to 21°C were determined. Heat and freeze–thaw resistance of E. coli O157:H7 and nonpathogenic E. coli was enhanced after acid adaptation and starvation. Following cold stress, heat resistance of E. coli O157:H7 and nonpathogenic E. coli was decreased, while freeze–t
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40

Weber, Courtney A., Gloria A. Moore, Zhanao Deng, and Fred G. Gmitter. "Mapping Freeze Tolerance Quantitative Trait Loci in a Citrus grandis × Poncirus trifoliata F1 Pseudo-testcross Using Molecular Markers." Journal of the American Society for Horticultural Science 128, no. 4 (2003): 508–14. http://dx.doi.org/10.21273/jashs.128.4.0508.

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Mapping quantitative trait loci (QTL) associated with freeze tolerance was accomplished using a Citrus grandis (L.) Osb. × Poncirus trifoliata (L.) Raf. F1 pseudo-testcross population. A progeny population of 442 plants was acclimated and exposed to temperatures of -9 °C and -15 °C in two separate freeze tests. A subpopulation of 99 progeny was genotyped for random amplified polymorphic DNA (RAPD), cleaved amplified polymorphic sequence (CAPS), sequence characterized amplified region (SCAR), and sequence tagged site (STS) markers to produce a linkage map for each parent. Potential QTL were ide
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Weber, Courtney A., Gloria A. Moore, Zhanao Deng, and Fred G. Gmitter. "Mapping Freeze Tolerance Quantitative Trait Loci in a Citrus grandis × Poncirus trifoliata F1 Pseudo-testcross Using Molecular Markers." Journal of the American Society for Horticultural Science 128, no. 4 (2003): 508–14. https://doi.org/10.21273/jashs.128.4.508.

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Mapping quantitative trait loci (QTL) associated with freeze tolerance was accomplished using a Citrus grandis (L.) Osb. × Poncirus trifoliata (L.) Raf. F1 pseudo-testcross population. A progeny population of 442 plants was acclimated and exposed to temperatures of -9 °C and -15 °C in two separate freeze tests. A subpopulation of 99 progeny was genotyped for random amplified polymorphic DNA (RAPD), cleaved amplified polymorphic sequence (CAPS), sequence characterized amplified region (SCAR), and sequence tagged site (STS) markers to produce a linkage map for each parent. Potential QTL were ide
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Min, Kyungwon, and Sang-Ryong Lee. "Exogenous Salicylic Acid Alleviates Freeze-Thaw Injury of Cabbage (Brassica oleracea L.) Leaves." Sustainability 13, no. 20 (2021): 11437. http://dx.doi.org/10.3390/su132011437.

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Freezing tolerance and physiological/biochemical changes were investigated for cabbage (Brassica oleracea L. ‘Myeong-Sung’) leaves treated with 0.5 mM salicylic acid (SA) by sub-irrigation. SA treatment did not interfere with leaf-growth (fresh/dry weight, and leaf-area), rather promoted growth (leaf-area) as compared to the control. Temperature-controlled, laboratory-based freeze-thaw assays revealed that SA-treated leaves were more freeze-tolerant than controls as evident by less ion-leakage as well as malondialdehyde content after freeze-thaw stress treatments (−2.5 and −3.5 °C). SA treatme
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Yu, Yifei, YaJing Wu, Wenfei Liu, Jun Liu, and Ping Li. "Integration of Metabolome and Transcriptome Reveals the Major Metabolic Pathways and Potential Biomarkers in Response to Freeze-Stress Regulation in Apple (Malus domestica)." Metabolites 13, no. 8 (2023): 891. http://dx.doi.org/10.3390/metabo13080891.

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Freezing stress is the main factor affecting the normal growth and distribution of plants. The safe overwintering of a perennial deciduous plant is a crucial link to ensuring its survival and yield. However, little is known about the molecular mechanism of its gene regulation metabolites as related to its freeze-tolerance. In order to enhance our comprehension of freeze-tolerance metabolites and gene expression in dormant apple trees, we examined the metabolic and transcriptomic differences between ‘Ralls’ and ‘Fuji’, two apple varieties with varying degrees of resistance to freezing. The resu
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Anderson, Jeffrey A., and Charles M. Taliaferro. "Freeze Tolerance of Seed-Producing Turf Bermudagrasses." Crop Science 42, no. 1 (2002): 190. http://dx.doi.org/10.2135/cropsci2002.0190.

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Anderson, Jeffrey A., and Charles M. Taliaferro. "Freeze Tolerance of Seed‐Producing Turf Bermudagrasses." Crop Science 42, no. 1 (2002): 190–92. http://dx.doi.org/10.2135/cropsci2002.1900.

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Phillips, K. "FREEZE-TOLERANCE EXTENDED BY RAPID COLD HARDENING." Journal of Experimental Biology 209, no. 3 (2006): ii. http://dx.doi.org/10.1242/jeb.02040.

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Walters, K. R., T. Sformo, B. M. Barnes, and J. G. Duman. "Freeze tolerance in an arctic Alaska stonefly." Journal of Experimental Biology 212, no. 2 (2008): 305–12. http://dx.doi.org/10.1242/jeb.020701.

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Toxopeus, Jantina, Jacqueline E. Lebenzon, Alexander H. McKinnon, and Brent J. Sinclair. "Freeze tolerance of Cyphoderris monstrosa (Orthoptera: Prophalangopsidae)." Canadian Entomologist 148, no. 6 (2016): 668–72. http://dx.doi.org/10.4039/tce.2016.21.

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AbstractThe great grig, Cyphoderris monstrosa Uhler (Orthoptera: Prophalangopsidae), is a large (20–30 mm, >1 g), nocturnal ensiferan that inhabits montane coniferous forests in northwestern North America. Cyphoderris monstrosa overwinters as a late instar nymphs, but its cold tolerance strategy has not previously been reported. We collected nymphs from near Kamloops, British Columbia, Canada in late spring to determine their cold tolerance strategy. Cyphoderris monstrosa nymphs were active at low temperatures until they froze at −4.6±0.3 °C. The nymphs survived internal ice formation (i.e.
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Packard, Gary C., and Mary J. Packard. "Natural freeze-tolerance in hatchling painted turtles?" Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 134, no. 2 (2003): 233–46. http://dx.doi.org/10.1016/s1095-6433(02)00264-7.

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SELTZER, RICHARD. "NMR probes freeze tolerance in arctic insect." Chemical & Engineering News 65, no. 20 (1987): 28. http://dx.doi.org/10.1021/cen-v065n020.p028.

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