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1

FLORES, D. A., L. E. PHILLIP, D. M. VEIRA, and M. IVAN. "THE SIGNIFICANCE OF SILAGE PROTEIN DEGRADATION AND PLASMA AMINO ACID RATIOS IN THE CONTROL OF FOOD INTAKE BY LAMBS FED ENSILED AND FRESH ALFALFA." Canadian Journal of Animal Science 66, no. 4 (1986): 1029–38. http://dx.doi.org/10.4141/cjas86-113.

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Alfalfa (30% dry matter) was harvested with a particle length of 0.6 cm, conserved fresh (frozen) or as silage and fed to eight lambs during two 27-d periods. The ensiling process resulted in substantial degradation of lysine, threonine, histidine, arginine, tryptophan and tyrosine in the original herbage. Isoleucine, leucine and valine resisted degradation during ensilage. As a consequence of these changes, the ratios of tyrosine: large neutral amino acids and tyrosine: phenylalanine (TYR:LNAA and TYR:PHE) were markedly lower in ensiled compared to fresh alfalfa (0.035 vs. 0.051 and 0.235 vs.
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2

Rufino-Moya, Pablo José, Mireia Blanco, Juan Ramón Bertolín, and Margalida Joy. "Methane Production of Fresh Sainfoin, with or without PEG, and Fresh Alfalfa at Different Stages of Maturity is Similar but the Fermentation End Products Vary." Animals 9, no. 5 (2019): 197. http://dx.doi.org/10.3390/ani9050197.

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Alfalfa and sainfoin are high-quality forages with different condensed tannins (CT) content, which can be affected by the stage of maturity. To study the effects of CT on fermentation parameters, three substrates (alfalfa, sainfoin, and sainfoin+PEG) at three stages of maturity were in vitro incubated for 72 h. Sainfoin had greater total polyphenol and CT contents than alfalfa. As maturity advanced, CT contents in sainfoin decreased (p < 0.05), except for the protein-bound CT fraction (p > 0.05). The total gas and methane production was affected neither by the substrate nor by the stage
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3

LOUI, CINDY, GRIGOR GRIGORYAN, HAOHAO HUANG, LEE W. RILEY, and SANGWEI LU. "Bacterial Communities Associated with Retail Alfalfa Sprouts." Journal of Food Protection 71, no. 1 (2008): 200–204. http://dx.doi.org/10.4315/0362-028x-71.1.200.

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Fresh produce, including salad, is increasingly implicated in foodborne outbreaks. Although studies have been carried out to detect specific human pathogens from fresh produce, the total bacterial community associated with fresh produce is poorly understood. In this study, we characterized the bacterial community associated with alfalfa sprouts, using a culture-independent method. Four retail-purchased alfalfa sprout samples were obtained from different producers, and the bacterial community associated with each sample was determined by 16S rDNA profiling. Our results indicate that alfalfa spr
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4

Kang, Sang-Mo, Muhammad-Aaqil Khan, Muhammad Hamayun, et al. "Phosphate-Solubilizing Enterobacter ludwigii AFFR02 and Bacillus megaterium Mj1212 Rescues Alfalfa’s Growth under Post-Drought Stress." Agriculture 11, no. 6 (2021): 485. http://dx.doi.org/10.3390/agriculture11060485.

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Drought stress is a prevalent environmental stress that adversely affects agricultural industries worldwide. In this study, bacterial isolates, AFFR02 and Mj1212, showed tolerance to polyethylene glycol-induced (PEG) drought stress (approximately 15%) and possess strong phosphate-solubilizing capacity. Moreover, we investigated the plant growth attributes, chlorophyll content, and ion uptake in alfalfa plants (Medicago sativa L) inoculated with isolates AFFR02 and Mj1212 under drought stress. We observed that drought stress drastically affects alfalfa’s growth attributes: shoot length: SL (24.
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5

Makoni, N. F., M. A. G. von Keyserlingk, J. A. Shelford, L. J. Fisher, and R. Puchala. "Fractionation of fresh, wilted and ensiled alfalfa proteins." Animal Feed Science and Technology 41, no. 1 (1993): 1–13. http://dx.doi.org/10.1016/0377-8401(93)90090-7.

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6

McMahon, L. R., W. Majak, T. A. McAllister, et al. "Effect of sainfoin on in vitro digestion of fresh alfalfa and bloat in steers." Canadian Journal of Animal Science 79, no. 2 (1999): 203–12. http://dx.doi.org/10.4141/a98-074.

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The effects of sainfoin (Onobrychis viciifolia) on digestion of alfalfa (Medicago sativa) were investigated in vitro and in vivo. Fresh alfalfa and sainfoin were incubated in an artificial rumen (Rusitec) in ratios of 100:0, 75:25, 50:50, 25:75 and 0:100 (as-fed). Disappearances of dry matter and N from sainfoin were 77 and 65% of those from alfalfa, respectively. Protease and endoglucanase activities, NH3-N and methane production declined (P < 0.05) as sainfoin increased. Bacterial numbers and microbial outputs were unchanged (P > 0.05), but cells incorporated more 15NH3N as sainfoin in
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7

Gao, Run, Ying Luo, Shengyang Xu, et al. "Effects of Replacing Ensiled-Alfalfa with Fresh-Alfalfa on Dynamic Fermentation Characteristics, Chemical Compositions, and Protein Fractions in Fermented Total Mixed Ration with Different Additives." Animals 11, no. 2 (2021): 572. http://dx.doi.org/10.3390/ani11020572.

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Alfalfa (Medicago sativa) is one of the high protein ingredients of fermented total mixed ration (FTMR). Additionally, FTMR is widely used to satisfy the nutrition requirements of animals. This study was conducted to confirm the fermentation characteristics, chemical compositions and protein fractions changes when replacing ensiled-alfalfa with fresh-alfalfa in FTMR with additives. Three additives were separately applied to fresh-alfalfa total mixed ration (TMR) and ensiled-alfalfa TMR, including molasses (MOL), Lactobacillus plantarum (LP) and MOL plus LP (MOL+LP). The same volume of distille
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8

Kisvarga, Szilvia, Döme Barna, Szilvia Kovács, et al. "Fermented Alfalfa Brown Juice Significantly Stimulates the Growth and Development of Sweet Basil (Ocimum basilicum L.) Plants." Agronomy 10, no. 5 (2020): 657. http://dx.doi.org/10.3390/agronomy10050657.

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Fertilization management is a key issue in plant nutrition to produce plants with good quality and quantity. Deproteinized leaf juice or brown juice (BJ) is a by-product during the isolation of leaf protein from biomass crops such as alfalfa. The idea of using BJ as a biostimulant fits well in the aspect of circular economy since BJ is currently a problematic issue of the leaf protein production approach. Fractionation of one-kilogram fresh biomass results in approximately 500 cm3 BJ. Due to fast spoil of fresh BJ, if left on room temperature, it is found that fermentation of fresh BJ using la
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9

FLORES, D. A., L. E. PHILLIP, D. M. VEIRA, and M. IVAN. "DIGESTION IN THE RUMEN AND AMINO ACID SUPPLY TO THE DUODENUM OF SHEEP FED ENSILED AND FRESH ALFALFA." Canadian Journal of Animal Science 66, no. 4 (1986): 1019–27. http://dx.doi.org/10.4141/cjas86-112.

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A study was conducted with six sheep equipped with rumen and duodenal re-entrant cannulae to investigate differences in duodenal amino acid supply with fresh and ensiled alfalfa. The experiment was conducted as a cross-over design with two 12-d periods consisting of an adaptation phase (5 d) followed by feces collection (5 d), and finally on day 12, 24 h continuous collection of duodenal digesta and sampling of rumen fluid. During the experiment the sheep were restricted on feed to 52 g DM d−1 kg−0.75. Analyses of the forages revealed extensive breakdown of threonine, lysine and other basic am
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10

Addini, Safira Amalina, Bambang Suwignyo, and Chusnul Hanim. "Suplementation Alfalfa (Medicago sativa L.) in commercial feed on physic and chemical quality meat of hybrid duck." E3S Web of Conferences 200 (2020): 03012. http://dx.doi.org/10.1051/e3sconf/202020003012.

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The aimed of this study was to determine the effect of suplementation level of alfafa (Medicago sativa L.) on hybrid duck’s meat quality with basal diet commercial feed. This study used in vivo tecnique on 108 hybrid ducks. The research consisted of 3 treatments and 6 replications, each replication consisted of 6 ducks. The treatments were P1 = commercial feed + 0 % fresh alfafa, P2 = commercial feed + 5 % alfafa, P3 = commercial feed + 10 % fresh alfafa. Alfalfa was calculated in dry matter based but offered in the form as fed. Feed and water was offered ad libitum. The variables measured inc
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11

MAJAK, W., and J. W. HALL. "SODIUM AND POTASSIUM CONCENTRATIONS IN RUMINAL CONTENTS AFTER FEEDING BLOAT-INDUCING ALFALFA TO CATTLE." Canadian Journal of Animal Science 70, no. 1 (1990): 235–41. http://dx.doi.org/10.4141/cjas90-027.

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Postprandial fluctuation of ruminal cations was determined in cattle with different susceptibilities to alfalfa bloat. Rumen contents were sampled before feeding and at regular intervals after feeding fresh alfalfa to determine the changes in concentration of sodium (Na) and potassium (K). Levels of Na and K changed dramatically 1.5–2.5 h after feeding. When compared to prefeeding levels, Na concentrations were lower but K concentrations were higher immediately after feeding fresh alfalfa. When rates of change of the ion concentrations were examined during the 8-h postprandial period, differen
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12

Al-Karaki, Ghazi N., and M. Al-Hashimi. "Green Fodder Production and Water Use Efficiency of Some Forage Crops under Hydroponic Conditions." ISRN Agronomy 2012 (December 26, 2012): 1–5. http://dx.doi.org/10.5402/2012/924672.

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The objectives of this study were to evaluate five forage crops (alfalfa (Medicago sativa), barley (Hordeum vulgare), cowpea (Vigna unguiculata), sorghum (Sorghum bicolor), and wheat (Triticum aestivum)) for green fodder production and water use efficiency under hydroponic conditions. The experiment has been conducted under temperature-controlled conditions (24 ± 1°C) and natural window illumination at growth room of Soilless Culture Laboratory, Arabian Gulf University, Manama, Bahrain. The results showed that green forage can be produced in 8 days from planting to harvest using hydroponic tec
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13

Samur, Salnan Irba Novaela, Bambang Suwignyo, and Edi Suryanto. "The effect of Alfalfa (Medicago sativa L.) on different basal feeds for hybrid duck performance." E3S Web of Conferences 200 (2020): 03013. http://dx.doi.org/10.1051/e3sconf/202020003013.

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The aimed of this research was to determine effect of Supplementation of Alfalfa (Medicago sativa L.) on different basal feeds for hybrid duck performance. This research was conducted by in vivo method with 120 MA (Mojosari x Alabio) of hybrid ducks type. The method was being used in this research include 4 treatments and 6 repetitions with 5 ducks each repetition. The treatment consisteds of P1 = Commercial feed 100 %, P2 = Alternative feed 100 %, P3 = Commercial Feed 90 % + supplementation of fresh alfalfa 10 % and P4 = Alternative feed 90 % + supplementation of fresh alfalfa 10 %. Feed and
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14

Mariotti, Marco, Filippo Fratini, Domenico Cerri, et al. "Use of Fresh Scotta Whey as an Additive for Alfalfa Silage." Agronomy 10, no. 3 (2020): 365. http://dx.doi.org/10.3390/agronomy10030365.

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Scotta is a dairy industry waste obtained by ricotta cheese production. Because of its high availability and its high lactose content, scotta could be used as an additive to improve fermentation characteristics of alfalfa silage. Silage samples were obtained for 2 years from an alfalfa second cut, collecting the forage at three growth stages: mid bud (MB), early flowering (EF), and late flowering (LF). After wilting the forage at 38% dry matter (DM), four scotta doses were added (0, 75, 150, and 300 g/kg fresh forage) and the main chemical and microbiological characteristics were evaluated aft
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15

Majak, W., G. J. Garland, and T. J. Lysyk. "The effect of feeding hay before fresh alfalfa on the occurrence of frothy bloat in cattle." Canadian Journal of Animal Science 88, no. 1 (2008): 29–31. http://dx.doi.org/10.4141/cjas07073.

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Alfalfa or orchardgrass hay was fed to cattle before feeding fresh alfalfa herbage at the vegetative to early bud stage of growth to observe the subsequent impact on bloat. Alfalfa hay supplements reduced the incidence of bloat by a third in one trial, but no effect was seen in a second trial. In contrast, supplements of orchardgrass hay reduced the occurrence of bloat by > 90% in 2 yr of trials. Supplemental orchardgrass hay can be effective in the control of pasture bloat. Key words: Steers, frothy bloat, Medicago sativa L., Dactylis glomerata L., hay supplements
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16

Xie, Yixiao, Jinze Bao, Wenqi Li, et al. "Effects of Applying Lactic Acid Bacteria and Molasses on the Fermentation Quality, Protein Fractions and In Vitro Digestibility of Baled Alfalfa Silage." Agronomy 11, no. 1 (2021): 91. http://dx.doi.org/10.3390/agronomy11010091.

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Alfalfa sometimes cannot be harvested in time due to the rainy season. To improve the fermentation quality, protein quality and digestibility of alfalfa silage harvested late, Lactobacillus plantarum (LP) and molasses were applied in an actual production process in this study. Alfalfa harvested at the full bloom stage was ensiled with (1) distilled water (control), (2) 1 × 106 colony-forming units LP/g fresh matter, (3) 15 g molasses/kg fresh matter (M) or (4) LP + M (LPM) for 55 days. Alfalfa ensiled with LP and/or molasses showed significantly lower pH and ammonia nitrogen contents than the
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17

Tao, L., H. Zhou, N. F. Zhang, et al. "Changes in carbohydrate and protein fractions during ensiling of alfalfa treated with previously fermented alfalfa juice or lactic acid bacteria inoculants." Animal Production Science 58, no. 3 (2018): 577. http://dx.doi.org/10.1071/an15067.

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The effects of previously fermented juice (PFJ) prepared from alfalfa and lactic acid bacteria (LAB) inoculants on the dynamic changes of nutritive components in ensiled alfalfa after various ensiling periods were investigated by using the Cornell Net Carbohydrate and Protein System. The third-cut alfalfa was harvested at the budding stage, exposed to sunlight, weighed occasionally to estimate the dry matter (DM) content until the actual DM finally obtained was 347.8 g/kg fresh weight, and then chopped to 1–2-cm lengths. Chopped forages were treated with (1) distilled water (control), (2) alfa
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18

Makoni, N. F., J. A. Shelford, S. Nakai, L. J. Fisher, and W. Majak. "Characterization of Protein Fractions in Fresh, Wilted, and Ensiled Alfalfa." Journal of Dairy Science 76, no. 7 (1993): 1934–44. http://dx.doi.org/10.3168/jds.s0022-0302(93)77527-x.

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19

TALEBI, KHALIL. "Dissipation of Phosalone and Diazinon in Fresh and Dried Alfalfa." Journal of Environmental Science and Health, Part B: Pesticides, Food Contaminants, and Agricultural Wastes 41, no. 5 (2006): 595–603. http://dx.doi.org/10.1080/03601230600701759.

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20

Gagné, Serge, Claude Richard, Hélène Rousseau, and Hani Antoun. "Xylem-residing bacteria in alfalfa roots." Canadian Journal of Microbiology 33, no. 11 (1987): 996–1000. http://dx.doi.org/10.1139/m87-175.

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Bacteria were consistently isolated from the root and crown xylem of symptomless field-grown alfalfa plants. Most of the plants tested contained more than one bacterial genus. Pseudomonas spp. accounted for 52% of the 387 isolates identified and the fluorescent pseudomonads were the most frequent bacteria isolated. About 23% of the isolates were Erwinia-like bacteria. Bacterial population ranged from 6.0 × 103 to 4.3 × 104 CFU/g of fresh xylem, and was not affected by plant age or cultivar or by the sampling locations. The surface-sterilized seeds of the cultivars Iroquois and Titan were bacte
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Ribeiro, C. V. D. M., S. K. R. Karnati, and M. L. Eastridge. "Biohydrogenation of Fatty Acids and Digestibility of Fresh Alfalfa or Alfalfa Hay Plus Sucrose in Continuous Culture." Journal of Dairy Science 88, no. 11 (2005): 4007–17. http://dx.doi.org/10.3168/jds.s0022-0302(05)73087-3.

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22

Poudyal, Shital, and Valtcho D. Zheljazkov. "Irrigation with Coalbed Methane Co-Produced Water Reduces Forage Yield and Increases Soil Sodicity However Does Not Impact Forage Quality." Sustainability 13, no. 6 (2021): 3545. http://dx.doi.org/10.3390/su13063545.

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The extraction of coalbed methane produces a significant amount of coalbed methane co-produced water (CBMW). Coalbed methane co-produced water is often characterized by high levels of pH, total dissolved solids (TDS), sodium (Na) and bicarbonate (HCO−3) and if used for irrigation without treatment, it may be detrimental to the surrounding soil, plants and environment. CBMW ideally should be disposed of by reinjection into the ground, but because of the significant cost associated, CBMW is commonly discharged onto soil or water surfaces. This study was conducted to elucidate the effect of the C
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23

RAZI, Sabah, and Fatima Zohra KHADHIR. "EFFECTS OF SALINITY ON GROWTH AND PROLINE CONTENT ON BEAN AND ALFALFA." Journal of Applied Life Sciences and Environment 186, no. 2 (2021): 132–45. http://dx.doi.org/10.46909/journalalse-2021-013.

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Soil salinity is a major agricultural constraint in many countries. Plants’ response to salinity depends on the species and the salt concentration in the soil. The objective of this study was to test the resistance of three leguminous plants - common bean (variety ‘El-Jadida’), broad bean (variety ‘Claro de Luna’), and alfalfa (variety ‘Diamon’) - to several salinity rates and to show which one was more resistant to salt stress. This study was carried out under controlled conditions, with six NaCl concentrations (0, 1, 3, 6, and 9 g/kg) added to a mixture of potting soil and sand. A completely
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24

Ruh, Kathryn E., Bradley J. Heins, Isaac J. Salfer, Robert D. Gardner, and Marshall D. Stern. "Comparison of warm season and cool season forages for dairy grazing systems in continuous culture1." Translational Animal Science 2, no. 2 (2018): 125–34. http://dx.doi.org/10.1093/tas/txy014.

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AbstractThe objective of this study was to compare warm-season annual grasses to cool-season perennial (CSP) grasses for ruminal nutrient digestibility and N metabolism in a dual-flow continuous culture fermentation system. Dietary treatments were 1) fresh alfalfa, 2) CSP grasses and legumes, 3) brown-midrib sorghum-sudangrass (BMRSS), and 4) teff grass from an organic dairy production system. Eight dual-flow continuous culture fermenters were used during two consecutive 10-d periods consisting of 7 d for stabilization followed by 3 d of sampling. Fermenter samples were collected on days 8, 9,
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Li, G. Q., H. C. Huang, S. N. Acharya, and R. S. Erickson. "Biological Control of Blossom Blight of Alfalfa Caused by Botrytis cinerea Under Environmentally Controlled and Field Conditions." Plant Disease 88, no. 11 (2004): 1246–51. http://dx.doi.org/10.1094/pdis.2004.88.11.1246.

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Fungal and bacterial antagonists were tested for their inhibition of sporulation of Botrytis cinerea on detached alfalfa florets. Clonostachys rosea, Gliocladium catenulatum, and Trichoderma atroviride were evaluated for protecting young blossoms and pods of alfalfa from infection by B. cinerea in vitro. C. rosea was further tested to control pod rot and seed rot caused by B. cinerea under field conditions. The results showed that four of the tested antagonists, C. rosea, G. catenulatum, T. atroviride, and Trichothecium roseum, could inhibit sporulation by B. cinerea on detached alfalfa floret
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Whiting, C. M., T. Mutsvangwa, J. P. Walton, J. P. Cant, and B. W. McBride. "Effects of feeding either fresh alfalfa or alfalfa silage on milk fatty acid content in Holstein dairy cows." Animal Feed Science and Technology 113, no. 1-4 (2004): 27–37. http://dx.doi.org/10.1016/j.anifeedsci.2003.11.004.

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Wu, Zhe, Shengyang Xu, Ying Yun, Tingting Jia, and Zhu Yu. "Effect of 3-Phenyllactic Acid and 3-Phenyllactic Acid-Producing Lactic Acid Bacteria on the Characteristics of Alfalfa Silage." Agriculture 10, no. 1 (2019): 10. http://dx.doi.org/10.3390/agriculture10010010.

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In this study, an experiment was performed to evaluate the effect of lactic acid bacteria and 3-phenyllactic acid (PLA) on the fermentation quality and chemical composition of alfalfa silage. Several PLA-tolerant strains were screened from silages and identified. The selected strains (1 × 106 colony forming units/g fresh alfalfa) and PLA (1.0, 2.0, or 3.0 g/kg) were applied to alfalfa before ensiling. After 45 days of storage, the silages were unsealed and subjected to component analysis. Biochemical methods and 16S rDNA gene sequencing were used for the identification of the two strains as La
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Marinov-Serafimov, Plamen, Irena Golubinova, and Diana Marinova. "Allelopathic tolerance of alfalfa (Medicago sativa L.) varieties to dodder (Cuscuta epithymum L.)." Pesticidi i fitomedicina 32, no. 1 (2017): 51–59. http://dx.doi.org/10.2298/pif1701051m.

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Allelopathic effects of cold water extracts of Cuscuta epithymum L. on seed germination and initial development of Medicago sativa L. varieties were investigated under laboratory conditions at the Institute of Forage Crops, Pleven, during 2016-2017. It was found that the water extracts from dry biomass of C. epithymum had a considerably stronger inhibitory effect on the studied M. sativa varieties (IR 32.7-100.0 %), as compared to the extracts of fresh biomass (IR 0.2-40.5%). Depending on the kind of C. epithymum extract (fresh or dry parasitic weed biomass), IRs for seed germination of the te
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Hermanto, Hermanto, Bambang Suwignyo Bambang Suwignyo, and Nafiatul Nafiatul. "KUALITAS KIMIA DAN KANDUNGAN KLOROFIL TANAMAN ALFALFA (Medicago sativa L.) DENGAN LAMA PENYINARAN DAN DOSIS DOLOMIT YANG BERBEDA PADA TANAH REGOSOL." Buletin Peternakan 41, no. 1 (2017): 54. http://dx.doi.org/10.21059/buletinpeternak.v41i1.9831.

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This study aimed to analyze the quality of chemical and chlorophyll content of alfalfa plants with lenght of irradiation and dose of dolomite on the regosol soil. The experiment was conducted implemented in Greenhouse Laboratory Forage and Pasture Faculty of Animal Sciences University of Gadjah Mada, from May to September 2015. This study used a Completely Randomized Design (CRD) 3 x 3 factorial design with two factors treatments and four replications. The first factor is the dose of dolomite (D) with 3 treatment (D0 = without dolomite, D1 = dolomite 6 t/hectare or 90 g/polybag and D2= dolomit
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Wang, Musen, Run Gao, Marcia Franco, et al. "Effect of Mixing Alfalfa with Whole-Plant Corn in Different Proportions on Fermentation Characteristics and Bacterial Community of Silage." Agriculture 11, no. 2 (2021): 174. http://dx.doi.org/10.3390/agriculture11020174.

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The influence of mixing alfalfa with whole-plant corn in different proportions on the fermentation characteristics and bacterial community of silage was investigated. Alfalfa and whole-plant corn, harvested at dry matter content of 276.47 and 328.43 g/kg fresh weight, accordingly, were chopped to approximately 2 cm and mixed at ratios of 100:0 (C0, control), 80:20 (C20), 60:40 (C40), 40:60 (C60), 20:80 (C80) and 0:100 (C100) on a fresh weight basis, respectively. Silos of each treatment were produced in triplicate and anaerobically fermented in darkness for 100 days at room temperature (20–21
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Castillo, A. R., D. E. Beever, R. F. Gregoret, S. G. Onetti, and O. R. Quaino. "The effect of supplementary carbohydrate composition on lactational performance in dairy cows grazing alfalfa pasture." Proceedings of the British Society of Animal Science 1998 (1998): 203. http://dx.doi.org/10.1017/s0308229600034164.

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The rate of disimilation of the dietary protein fraction of fresh forages, especially fresh legumes, is rapid and often exceeds microbial requirements for ammonia [Beever, 1993]. Consequently total dietary nitrogen [N] utilisation is impaired due to increased absorption of ammonia from the rumen and excretion of urea via the kidneys. This experiment, as part of a larger co-ordinated study to examine N utilisation in alfalfa swards for milk production, considered the evaluation of different isoenergetic supplements formulated to contain contrasting carbohydrate components with respect to starch
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32

Hipskind, John D., and Nancy L. Paiva. "Constitutive Accumulation of a Resveratrol-Glucoside in Transgenic Alfalfa Increases Resistance to Phoma medicaginis." Molecular Plant-Microbe Interactions® 13, no. 5 (2000): 551–62. http://dx.doi.org/10.1094/mpmi.2000.13.5.551.

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Alfalfa (Medicago sativa) was transformed with a peanut (Arachis hypogaea) cDNA encoding resveratrol synthase (RS) transcriptionally regulated by an enhanced Cauliflower mosaic virus (CaMV) 35S promoter. Transgenic plants accumulated a new compound, not present in wild-type or vector-transformed alfalfa, that was identified as trans-resveratrol-3-O-β-D-glucopyranoside (RGluc) by high-pressure liquid chromatography (HPLC), UV, 1H- and 13C-nuclear magnetic resonance (NMR) analyses. RGluc concentration was highest in the youngest leaves (>15 μg per g fresh weight) and oldest stem internode seg
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Gajewski, Marek, Honorata Danilcenko, Zivile Taraseviciene, Paweł Szymczak, Anna Seroczyńska, and Jadwiga Radzanowska. "Quality Characteristics of Fresh Plant Sprouts and After Their Short-term Storage." Vegetable Crops Research Bulletin 68, no. 1 (2008): 155–66. http://dx.doi.org/10.2478/v10032-008-0014-3.

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Quality Characteristics of Fresh Plant Sprouts and After Their Short-term StorageSensory characteristics, carotenoids content and antioxidant activity of sprouted seeds of eight plant species were investigated. Seeds of lentil, broccoli, clover, amaranth, wheat, radish, pea, and alfalfa were germinated for 3 days in the dark, at the temperature of 25°C and then sprouted seeds were stored for the next 2 days in a refrigerator, in the dark, at the temperature of 4-5°C. Sensory quality was evaluated in the expert panel, with the quantitative descriptive analysis method (QDA), using 15 sensory att
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Chen, Tony H. H., and Frank S. C. Chen. "Relations between photoperiod, temperature, abscisic acid, and fall dormancy in alfalfa (Medicago sativa)." Canadian Journal of Botany 66, no. 12 (1988): 2491–98. http://dx.doi.org/10.1139/b88-338.

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Three alfalfa (Medicago sativa L.) cultivars differing in fall dormancy were examined for endogenous abscisic acid content in relation to fall dormancy. The alfalfa cultivars studied were 'Beaver' (dormant type), 'Lahonton' (semidormant type), and 'Moapa 69' (nondormant type). Alfalfa seedlings were grown in controlled-environment chambers set at a 16-h photoperiod (16 h light: 8 h dark) and 20:15 °C (light:dark) for 55 days. They were cut and then regrown under environmental conditions simulating summer and fall in western Canada. In leaf samples, the ABA level increased from about 200 in 3-w
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Owens, V. N., K. A. Albrecht, and R. E. Muck. "Protein degradation and ensiling characteristics of red clover and alfalfa wilted under varying levels of shade." Canadian Journal of Plant Science 79, no. 2 (1999): 209–22. http://dx.doi.org/10.4141/p98-034.

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The conversion of protein nitrogen (PN) to nonprotein nitrogen (NPN) in forages occurs rapidly and extensively during wilting and ensiling. The objectives of this study were to determine whether the amount of time between cutting and ensiling affects protein degradation in red clover (Trifolium pratense L.) and alfalfa (Medicago sativa L.) silage and to document pre- and post-ensiling characteristics of these two species. Forage from the second (24 August 1993), first (27 May 1994), and second (10 July 1995) growth cycles was harvested with hand clippers to a 5-cm stubble height between 09:00
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Lobo, Richard R., Marcos I. Marcondes, Paulo H. Rodrigues, Antonio Faciola, Rafael Pinheiro, and Ives C. Bueno. "PSXII-36 Modelling in vitro gas production kinetics of fresh alfalfa incubated with inocula from five ruminant species." Journal of Animal Science 97, Supplement_3 (2019): 427–28. http://dx.doi.org/10.1093/jas/skz258.847.

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Abstract The objective was to identify the non-linear model with the best fit for cumulative gas production from fermentation of fresh alfalfa, with or without tannin extract, incubated with rumen fluid from five different species of ruminants. Fifteen animals (Taurine and Zebuine cattle, water buffaloes, sheep and goats) were used as inoculum donors. During incubation, 500 mg of fresh alfalfa, with or without 150 mg of acacia tannin extract, were used as substrate in the semi-automated gas production technique. Experimental design was completely randomized in a factorial arrangement with five
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Grilli, Diego Javier, Jakub Mrázek, Kateřina Fliegerová, et al. "Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage." Livestock Science 191 (September 2016): 191–95. http://dx.doi.org/10.1016/j.livsci.2016.08.001.

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38

Liu, Xulei, Fuyao Liu, Tianhai Yan, Shenghua Chang, Metha Wanapat, and Fujiang Hou. "Cistanche deserticola Addition Improves Growth, Digestibility, and Metabolism of Sheep Fed on Fresh Forage from Alfalfa/Tall Fescue Pasture." Animals 10, no. 4 (2020): 668. http://dx.doi.org/10.3390/ani10040668.

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This study is targeted at evaluating whether C. deserticola addition promotes digestion, nitrogen and energy use, and methane production of sheep fed on fresh forage from alfalfa/tall fescue pastures. The sheep feeding trial was conducted with four addition levels with C. deserticola powder, and a basal diet of fresh alfalfa (Medicago sativa) and tall fescue (Festuca arundinacea). Addition levels of 4% and 6% improved average body weight gain (BWG) by 215.71 and 142.86 g/d, and feed conversion ratio (FCR) by 0.20 and 0.14, respectively. Digestibility of dry matter (DM), organic matter (OM), ne
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Wambacq, Eva, Joos Pieter Latré, and Geert Haesaert. "The effect of Lactobacillus buchneri inoculation on the aerobic stability and fermentation characteristics of alfalfa-ryegrass, red clover and maize silage." Agricultural and Food Science 22, no. 1 (2013): 127–36. http://dx.doi.org/10.23986/afsci.6711.

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Aerobic spoilage of silages occurs frequently and is undesirable because it reduces both its nutritive and hygienic quality. Silage inoculants containing heterofermentative lactic acid bacteria, like Lactobacillus buchneri, have already been proven to improve aerobic stability by augmented production of acetic acid, which inhibits yeasts. In this study, the effect of L. buchneri on fermentation characteristics and aerobic stability of alfalfa-ryegrass silage, red clover silage and maize silage was assessed using microsilos. Two dosages, 1×105 and 3×105 cfu g-1 of fresh matter, were compared to
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40

Ćwintal, M., and A. Dziwulska-Hunek. "Effect of electromagnetic stimulation of alfalfa seeds." International Agrophysics 27, no. 4 (2013): 391–401. http://dx.doi.org/10.2478/intag-2013-0009.

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Abstract In the conducted experiments the effect of presowing He-Ne laser light, magnetic field stimulation or the combination of these two factors of alfalfa seeds on the field emergence, structure and yields in the year of sowing and during three following years of full land use were studied. The examined factors had a significant effect on the number of shoots per 1 m2, plant height, mass of shoots, fresh and dry mass. Electromagnetic stimulation resulted in a significant increase in alfalfa seeds emergence (from 35% - control to 47.8% - magnetic field), number of shoots per 1m2 (from 608 -
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MAJAK, W., R. E. HOWARTH, and P. NARASIMHALU. "CHLOROPHYLL AND PROTEIN LEVELS IN BOVINE RUMEN FLUID IN RELATION TO ALFALFA PASTURE BLOAT." Canadian Journal of Animal Science 65, no. 1 (1985): 147–56. http://dx.doi.org/10.4141/cjas85-015.

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Rumen-fistulated cattle were fed fresh alfalfa herbage daily during three growing seasons. Two hours after feeding the incidence of bloat and of ruminal frothiness was recorded and samples of feed and rumen fluid were collected for analyses of chlorophyll and soluble protein. These constituents were examined in relation to the bloat-causing potential of the alfalfa and in relation to the occurrence of froth in rumen contents. Chlorophyll in rumen fluid was higher on days when the alfalfa caused bloat, compared to days when bloat did not occur (P < 0.01). Chlorophyll was also higher in froth
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Dziwulska-Hunek, Agata, Agnieszka Kornarzyńska-Gregorowicz, Agnieszka Niemczynowicz, and Arkadiusz Matwijczuk. "Influence of Electromagnetic Stimulation of Seeds on the Photosynthetic Indicators in Medicago sativa L. Leaves at Various Stages of Development." Agronomy 10, no. 4 (2020): 594. http://dx.doi.org/10.3390/agronomy10040594.

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The paper explores the impact of electromagnetic stimulation of Ulstar alfalfa seeds on fresh mass yield, the quantum efficiency of the plants’ photochemical reactions, and the content of photosynthetic pigments in leaves. Before sowing, seeds were subjected to electromagnetic stimulation in the following configurations: control (C), no stimulation; stimulation with He–Ne laser light with the wavelength of 632.8 nm, surface power density of 3 mW·cm−2 and exposition time of 1 min (L1) or 5 min (L5); stimulation with alternating magnetic field with the induction of 30 mT and exposition time of 1
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Giorgio, Daniela, Adriana Di Trana, Paola Di Gregorio, et al. "Oxidative Status of Goats with Different CSN1S1 Genotypes Fed ad Libitum with Fresh and Dry Forages." Antioxidants 9, no. 3 (2020): 224. http://dx.doi.org/10.3390/antiox9030224.

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Forty late-lactation Girgentana goats were used to study the effect of diets fed ad libitum and αS1-casein (CSN1S1) genotype on redox balance. The goats genotyped at CSN1S1 locus (A/A, A/F) were subjected to four feeding treatments different for percentage inclusion of dry and fresh forage: DAF100 (98% of Dry Alfalfa Forage), DAF65 (65% of Dry Alfalfa Forage), FSF100 (100% of Fresh Sulla Forage) and FSF65 (65% of Fresh Sulla Forage). Blood samples were analyzed for superoxide dismutase (SOD) and glutathione peroxidase (GPX) activity, reactive oxygen metabolites (ROMs), biological antioxidant p
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Titi, H. H., S. I. Azzam, and M. A. Alnimer. "Effect of protected methionine supplementation on milk production and reproduction in first calf heifers." Archives Animal Breeding 56, no. 1 (2013): 225–36. http://dx.doi.org/10.7482/0003-9438-56-022.

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Abstract. Sixty Holstein heifers (30 days in milk) were used to examine the effects of protected methionine on milk production, milk composition and reproduction performance up to 100 days post-partum. Heifers were randomly divided into two dietary treatment groups (30 each) receiving either 14 or 16 % crude protein. Heifers of each treatment group were assigned into three subgroups with a daily supplementation of 0, 15 or 25 g/hd/d of rumen protected methionine. Diet fed was a total mixed ration consisting of fresh alfalfa, corn silage and a concentrate mixture. In addition, alfalfa hay was o
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Wang, Y., P. Frutos, M. Y. Gruber, H. Ray, and T. A. McAllister. "In vitro ruminal digestion of anthocyanidin-containing alfalfa transformed with the maize Lc regulatory gene." Canadian Journal of Plant Science 86, no. 4 (2006): 1119–30. http://dx.doi.org/10.4141/p06-001.

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In vitro ruminal digestion was conducted on novel “purple-green” alfalfa forage that accumulated anthocyanidin and proanthocyanidins through the expression of a maize bHLH anthocyanidin regulatory gene (Lc). The Lc-transgenic genotypes were compared with parental (non-transformed, NT) alfalfa in their in vitro ruminal fermentation, dry matter (DM) and N disappearances, and DM degradability. The transgenic genotypes expressed the Lc gene at different levels when grown under high light conditions, resulting in anthocyanidin contents as high as 136 g g-1 DM. Lc genotypes had lower true DM disappe
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Michálková, Jaroslava, Daniel Bíro, Miroslav Juráček, Milan Šimko, and Branislav Gálik. "Changes in amino acid profile of alfalfa silage preserved by chemical and biological additives during fermentation." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 57, no. 5 (2009): 213–20. http://dx.doi.org/10.11118/actaun200957050213.

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Changes in amino acid profile of alfalfa silage preserved with chemical or biological additives were studied in fresh and wilted silage. The chemical additive was formic acid and the biological additive consisted of Lactobacillus rhamnosus, L. plantarum, L. brevis, L. buchneri and Pediococcus pentosaceus. Second cut alfalfa (Medicago sativa L.) was harvested at the bloom stage, ensiled in mini silos (15 dm3) and fermented at 20–23 °C for 12 weeks. The dry matter of the fresh silage was 228 g . kg−1 and 281.6 g . kg−1 for the wilted before ensiling. The amino acid content was estimated by using
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47

Hunde, Yedilaklil, Jamie Patton, Dennis Padgitt, and David Vlieger. "Particle Size Influences Preferential Consumption of Hay and Baleage in Total Mixed Dairy Rations." Transactions of the Missouri Academy of Science 42, no. 2008 (2008): 45–49. http://dx.doi.org/10.30956/0544-540x-42.2008.45.

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Lactating cows require complete balanced rations to meet their high nutritional requirements and to maximize milk and milk fat production. Total mixed rations (TMR) were developed to meet these nutritional needs by thoroughly mixing necessary feedstuffs into a single ration; hence providing a balanced diet in each bite. However, the actual nutrient and fiber concentration consumed by cows can dramatically differ from that of the ration due to selective feeding. The objectives of this study were to 1) evaluate the palatability of alfalfa hay TMR and alfalfa baleage TMR and 2) determine the effe
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BOŽIČKOVIĆ, A., G. GRUBIĆ, J. VERBIČ, T. ŽNIDARŠIČ, N. DJORDJEVIĆ, and B. STOJANOVIĆ. "A modified method for assessment of the morphological stage of development as a predictor of alfalfa herbage chemical composition and nutritive value." Journal of Agricultural Science 151, no. 4 (2013): 590–98. http://dx.doi.org/10.1017/s0021859613000129.

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SUMMARYThe aim of the current work was to investigate the possibility of modifying the existing mean stage by weight (MSW) system for evaluating the average development stage in alfalfa. The modification was performed with the aim of providing a simplified system that may be used to evaluate the alfalfa development stage and to predict its nutritive value for ruminants. The suggested modification consists of designating an MSW value on the basis of the fresh weight of all morphological stages in a fresh green plant, as opposed to the original method which is based on weighing all morphological
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Makoni, N. F., J. A. Shelford, and L. J. Fisher. "Initial Rates of Degradation of Protein Fractions from Fresh, Wilted, and Ensiled Alfalfa." Journal of Dairy Science 77, no. 6 (1994): 1598–603. http://dx.doi.org/10.3168/jds.s0022-0302(94)77102-2.

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Collins, Michael. "Composition and Yields of Alfalfa Fresh Forage, Field Cured Hay, and Pressed Forage." Agronomy Journal 82, no. 1 (1990): 91–95. http://dx.doi.org/10.2134/agronj1990.00021962008200010021x.

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