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Dissertations / Theses on the topic 'FRET sensor'

Author: Grafiati

Published: 4 June 2021

Last updated: 10 March 2023

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1

Shi, Lifang. "Luminescent Quantum dots for Cellular Analysis." ScholarWorks@UNO, 2007. http://scholarworks.uno.edu/td/597.

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Luminescent quantum dots have attracted great interest in recent years among biological researchers since they provide solutions to problems associated with use of organic fluorophores in cellular studies. Quantum dots show high photostability, high emission quantum yield, narrow and symmetric emission peaks and size-dependent wavelength tunability. The objective of my PhD studies was to develop CdSe/ZnS quantum dot-based probes and utilize them in cellular assays. The first phase of the work was to develop luminescent quantum dot fluorescence resonance energy transfer (FRET) based probes for

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2

Li, Xuelian. "USING CONJUGATED POLYMERS AS BIOLOGICAL SENSOR BASED ON FLUORESCENCE RESONANCE ENERGY TRANSFER." OpenSIUC, 2011. https://opensiuc.lib.siu.edu/dissertations/321.

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J E. coli On-Off &ldquo, &rdquo, °, &ndash The specific objectives of the work presented in this dissertation are to design novel molecular sensors based on fluorescence resonance energy transfer (FRET) between fluorophore (donor) and polydiacetylene (PDA, acceptor) for selective detection of biomolecules in solution. The work described in this dissertation is divided into three sections. In the first section, we report here two novel systems where the rate of energy transfer is based on changes in the spectral overlap between the emission of the donor and the absorption of the acceptor (J) as

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3

Wren, David Andrew. "Measuring calcium in Pseudomonas aeruginosa using a genetically encoded fluorescence resonance energy transfer (FRET) sensor." Connect to online resource, 2008. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:1460883.

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4

Bolbat, Andrey [Verfasser], and Carsten [Akademischer Betreuer] Schultz. "Genetically encoded FRET sensor to detect double-stranded RNA during viral infection / Andrey Bolbat ; Betreuer: Carsten Schultz." Heidelberg : Universitätsbibliothek Heidelberg, 2019. http://d-nb.info/1191760537/34.

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5

Wen, Lai [Verfasser], and Robert [Akademischer Betreuer] Feil. "Generation and characterization of FRET-based cGMP sensor knock-in mice for cGMP imaging / Lai Wen ; Betreuer: Robert Feil." Tübingen : Universitätsbibliothek Tübingen, 2014. http://d-nb.info/1198119799/34.

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6

König, Benjamin [Verfasser]. "Establishment and application of a FRET sensor for VRAC activity reveals a key role for diacylglycerol signaling in channel regulation / Benjamin König." Berlin : Freie Universität Berlin, 2020. http://d-nb.info/1219508322/34.

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7

Greotti, Elisa. "Development of new tools to explore organelle calcium dynamics in vivo: a new fret-based calcium sensor and a mitochondria targeted channelrhodopsin." Doctoral thesis, Università degli studi di Padova, 2015. http://hdl.handle.net/11577/3424766.

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The first part of this thesis aims to develop new tools to explore mitochondrial Ca2+ dynamic in vivo by the improvement of a mitochondria targeting Cameleon probes and by creating a mitochondria targeted Channelrhodopsin. Genetically encoded calcium indicators (GECIs) allow quantitative Ca2+ measurements in different experimental models. Organelle-specific targeting signals are fused with the GECI’s sequence, achieving selective targeting to a specific organelle or cytoplasmic domain. Moreover, GECI’s coding sequences can be placed under the control of tissue specific or inducible promoters

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8

Zhao, Zhijian [Verfasser], Frank Akademischer Betreuer] Müller, and Marc [Akademischer Betreuer] [Spehr. "Towards monitoring nitric oxide mediated cGMP metabolism in mouse retinal neurons using a FRET-based genetically encoded sensor / Zhijian Zhao ; Frank Müller, Marc Spehr." Aachen : Universitätsbibliothek der RWTH Aachen, 2015. http://d-nb.info/1127589660/34.

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9

Zhao, Zhijian Verfasser], Frank [Akademischer Betreuer] Müller, and Marc [Akademischer Betreuer] [Spehr. "Towards monitoring nitric oxide mediated cGMP metabolism in mouse retinal neurons using a FRET-based genetically encoded sensor / Zhijian Zhao ; Frank Müller, Marc Spehr." Aachen : Universitätsbibliothek der RWTH Aachen, 2015. http://d-nb.info/1127589660/34.

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10

Haridoss, Sujithera. "In vivo assessment of focal adhesion kinase (FAK) activity in breast cancer cells using fluorescence resonance energy transfer (FRET) sensor and confocal laser scanning microscope (CLSM)." Thesis, Högskolan i Skövde, Institutionen för hälsa och lärande, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-15706.

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Focal Adhesion Kinase (FAK) is essential for cell migration and plays an important role in tumor metastasis. However, the complex intermolecular and intramolecular interactions that regulate FAK activity at the focal adhesion remain unresolved. We have engineered a toolbox of Fluorescence Resonance Energy Transfer (FRET) sensors for the assessment of FAK activity in human breast cancer cells (MCF-7). Major activity of cancerous cells is drastically growth of the cell in an uncontrollable manner in such cases our human anatomy system normally consists of cell growth activity. The important prot

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11

DOCCULA, FABRIZIO GANDOLFO. "TWO CLADE III GLUTAMATE RECEPTOR-LIKE ISOFORMS INVERSELY REGULATE LOCAL AND LONG-DISTANCE CA2+ SIGNALLING IN ARABIDOPSIS THALIANA." Doctoral thesis, Università degli Studi di Milano, 2019. http://hdl.handle.net/2434/625277.

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In the central nervous system, ionotropic Glutamate Receptors (iGluRs) are tetrameric ligand-gated non-selective cation channels. They have been linked to learning and memory as well as to neurodegenerative pathologies such as Alzheimer disease. Thus, they are among the best characterized channels in animals. Animals and plants share this class of proteins. Plant Glutamate Receptors-like (GLRs) have been implicated in stomata movement regulation, pollen tube growth, long-distance signalling, root development and defence against pathogens. However, to date few details are known about their basi

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12

Blagoi, Gabriela. "Fluorescence resonance energy transfer (FRET) based sensors for bioanalysis." ScholarWorks@UNO, 2004. http://louisdl.louislibraries.org/u?/NOD,146.

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Thesis (Ph. D.)--University of New Orleans, 2004.<br>Title from electronic submission form. "A dissertation ... in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Chemistry."--Dissertation t.p. Vita. Includes bibliographical references.

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13

Johansson, Jonas, and Daniel Petersson. "Torque Sensor Free Power Assisted Wheelchair." Thesis, Halmstad University, School of Information Science, Computer and Electrical Engineering (IDE), 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:hh:diva-656.

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<p>A power assisted wheelchair combines human power, which is delivered by the arms through the pushrims, with electrical motors, which are powered by a battery. Today’s electric power assisted wheelchairs use force sensors to measure the torque exerted on the pushrims by the user. The force sensors in the pushrims are rather expensive and this approach also makes the wheels a little bit clumsy. The objective with this project is to find a new, better and cheaper solution that does not use expensive force sensors in the pushrims. The new power assisted wheelchair will instead only rely on its

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14

Thompson, Liz. "A label free DNA hybridization sensor." Thesis, Georgia Institute of Technology, 2002. http://hdl.handle.net/1853/26968.

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15

Baghalian, Amin. "Detecting Structural Defects Using Novel Smart Sensory and Sensor-less Approaches." FIU Digital Commons, 2017. https://digitalcommons.fiu.edu/etd/3560.

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Monitoring the mechanical integrity of critical structures is extremely important, as mechanical defects can potentially have adverse impacts on their safe operability throughout their service life. Structural defects can be detected by using active structural health monitoring (SHM) approaches, in which a given structure is excited with harmonic mechanical waves generated by actuators. The response of the structure is then collected using sensor(s) and is analyzed for possible defects, with various active SHM approaches available for analyzing the response of a structure to single- or multi-f

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16

Harvey, Raven Mariah. "Development of genetically encoded heme sensors." Thesis, Georgia Institute of Technology, 2015. http://hdl.handle.net/1853/53607.

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Due to the biological importance of heme and its implication in various disease states, uncovering how it is transported throughout the cell is of vital importance. Some of the strongest in vivo tools present in the literature are FRET-based sensors using a number of chromophores that are optimized and expanded from GFP. In order to elucidate the movement of heme throughout the cell, GFP FRET -based heme sensors were designed, expressed, and purified to be further characterized in vitro. This series of heme sensors were expressed in Saccharomyces cerevisiae to monitor the in vivo movement of h

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17

Zhang, Dian. "Transceiver-free object tracking in wireless sensor networks /." View abstract or full-text, 2006. http://library.ust.hk/cgi/db/thesis.pl?COMP%202006%20ZHANG.

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18

Frey, Alexander [Verfasser]. "Fully Electronic CMOS DNA Sensor Chip / Alexander Frey." Aachen : Shaker, 2011. http://d-nb.info/1080765115/34.

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19

Lim, Shen Hin Mechanical &amp Manufacturing Engineering Faculty of Engineering UNSW. "Calibration-free image sensor modelling: deterministic and stochastic." Awarded by:University of New South Wales. Mechanical & Manufacturing Engineering, 2009. http://handle.unsw.edu.au/1959.4/44563.

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This dissertation presents the calibration-free image sensor modelling process applicable for localisation, such that these are robust to changes in environment and in sensor properties. The modelling process consists of two distinct parts, which are deterministic and stochastic techniques, and is achieved using mechanistic deconvolution, where the sensor???s mechanical and electrical properties are utilised. In the deterministic technique, the sensor???s effective focal length is first estimated by known lens properties, and is used to approximate the lens system by a thick lens and its prop

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20

Fernandes, Ricardo Dias. "Design of a battery-free wireless sensor node." Master's thesis, Universidade de Aveiro, 2010. http://hdl.handle.net/10773/5616.

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Mestrado em Engenharia Electrónica e Telecomunicações<br>Redes de sensores sem fios são hoje em dia utilizadas numa grande variedade de aplicações, o que justifica o facto de que os sensores que as constituem sejam igualmente diversificados. Ainda assim, quase todos eles dependem de baterias, as quais ficam sem carga normalmente muito antes do fim de vida dos restantes componentes. Para além disso, o tamanho da baterias é neste momento um impeditivo à redução do tamanho dos sensores. Uma forma de contornar estes problemas consiste em retirar as baterias dos dispositivos e em alternativa

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21

Allen, Graham Scott. "Optical sensor design for advanced drag-free satellites /." May be available electronically:, 2009. http://proquest.umi.com/login?COPT=REJTPTU1MTUmSU5UPTAmVkVSPTI=&clientId=12498.

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22

Nawaz, Sarfraz Computer Science &amp Engineering Faculty of Engineering UNSW. "Anchor free localization for ad-hoc wireless sensor networks." Publisher:University of New South Wales. Computer Science & Engineering, 2008. http://handle.unsw.edu.au/1959.4/41427.

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Wireless sensor networks allow us to instrument our world in novel ways providing detailed insight that had not been possible earlier. Since these networks provide an interface to the physical world, it is necessary for each sensor node to learn its location in the physical space. The availability of location information at individual sensor nodes allows the network to provide higher layer services such as location stamped event reporting, geographic routing, in-network processing etc. A wide range of these sensor network protocols do not require absolute node coordinates and can work with rel

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23

Wang, Yunmiao. "Microgap Structured Optical Sensor for Fast Label-free DNA Detection." Thesis, Virginia Tech, 2011. http://hdl.handle.net/10919/32875.

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DNA detection technology has developed rapidly due to its extensive application in clinical diagnostics, bioengineering, environmental monitoring, and food science areas. Currently developed methods such as surface Plasmon resonance (SPR) methods, fluorescent dye labeled methods and electrochemical methods, usually have the problems of bulky size, high equipment cost and time-consuming algorithms, so limiting their application for in vivo detection. In this work, an intrinsic Fabry-Perot interferometric (IFPI) based DNA sensor is presented with the intrinsic advantages of small size, low cost

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24

Gui, Linqing. "Improvement of Range-free Localization Systems in Wireless Sensor Networks." Thesis, Toulouse, INSA, 2013. http://www.theses.fr/2013ISAT0005/document.

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Dans le contexte des réseaux de capteurs sans fil, la technique de localisation ”range-free” est plus efficiente, par rapport au principe ”range-based”. Par conséquent, nous avons focalisé nos travaux de cette thèse sur les techniques ”range-free”. Afin de permettre à chaque noeud mobile ou normal de choisir son propre algorithme de localisation, nous avons proposé un mécanisme adapté en scindant les noeuds normaux en deux classes: les noeuds de la première classe ont au moins 3 ancres voisines, alors que les noeuds de la deuxième classe ont moins de trois ancres voisines. Pour les noeuds norm

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25

Garcia-Fernández, Victor. "Use of Gravity Sensors for Free Space Orientation." Thesis, KTH, Skolan för informations- och kommunikationsteknik (ICT), 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-174845.

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This thesis deals with different approaches to filtering the signal output of a three axis accelerometerfor free space orientation, that is, finding the orientation of a sensor relative to gravity. Theplatform this orientation system is to be developed for is a low-power, high-efficiency fixed-pointmath microprocessor, therefore efficiency and mathematical operation precision are factors thatalso need to be taken into consideration in this work.This is an exploratory work which goals are to analize potential filtering solutions for free spaceorientation with a three-axis accelerometer, develop

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26

Youssef, Adel Amin Abdel Azim. "Salam a scalable anchor-free localization algorithm for wireless sensor networks /." College Park, Md. : University of Maryland, 2006. http://hdl.handle.net/1903/3496.

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Thesis (Ph. D.) -- University of Maryland, College Park, 2006.<br>Thesis research directed by: Computer Science. Title from t.p. of PDF. Includes bibliographical references. Published by UMI Dissertation Services, Ann Arbor, Mich. Also available in paper.

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27

Nelson, Justin T. (Justin Ttheodore). "Carbon nanotube-based sensors for label-free protein detection." Thesis, Massachusetts Institute of Technology, 2015. http://hdl.handle.net/1721.1/98162.

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Thesis: S.M., Massachusetts Institute of Technology, Department of Chemical Engineering, 2015.<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references (pages 31-40).<br>Optical biosensors based on fluorescent single-walled carbon nanotubes (SWNT) are a promising alternative to conventional biosensors due to the exceptional photophysical properties of SWNT. Such sensors can enable highly-sensitive, selective, and real-time detection of biological analytes. However, important questions regarding sensor fabrication and reproducibility must be addressed for these sensors to

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28

Ning, Tong. "Evaluation of a Microwave Sensor for Powder Process Control." Thesis, University of Gävle, Department of Technology and Built Environment, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-703.

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<p>In this thesis work, a free space microwave sensing technique to be used for power process control was investigated. Evaluation of the possibility to apply this sensing technique for determination of permittivity properties is the primary objective. Further these properties could be related to the material physical properties such as moisture content and density. Due to the fact that the permittivity properties of the material under measurement determine its measured S-parameters, such were performed throughout the whole work for the calculation of materials properties. Free space type of m

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29

Krsmanovic, Dalibor. "High temperature ultrasonic gas flow sensor based on lead free piezoelectric material." Thesis, University of Cambridge, 2011. https://www.repository.cam.ac.uk/handle/1810/245065.

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The review of current technologies for measurement of gas velocity in stack flow applications is undertaken and it is shown that the ultrasonic time-of-flight method is the most suitable and offers a number of advantages over alternatives. Weakness of current piezoelectric based transducers are identified as the inability to operate at temperatures above 400 °C due to limitation of piezoelectric materials used, and a case for development of an alternative high temperature material is put forward. A novel and highly enhanced, lead free piezoelectric material, suitable for continuous operation a

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30

Boulton, SarahJayne. "Integrated free radical sensor systems for investigation of cellular models of disease." Thesis, University of Newcastle Upon Tyne, 2012. http://hdl.handle.net/10443/1427.

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Reactive oxygen species (ROS) including superoxide (O2̄), nitric oxide (NO•) and hydrogen peroxide (H2O2) are thought to play a central role in many disease processes. This thesis details the development of novel optical and electrochemical sensor platforms for the analysis of ROS. These technologies were established in response to the current limitations of existing techniques to enable greater understanding of the role of ROS in cellular pathology. The overproduction of O2̄ by mitochondria has been linked to the initiation of disease processes. Specifically, defects in the mitochondrial elec

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31

Chandra, Sudeshna, Christian Gäbler, Christian Schliebe, Heinrich Lang, and Dhirendra Bahadur. "Fabrication of a label-free electrochemical immunosensor using a redox active ferrocenyl dendrimer." Universitätsbibliothek Chemnitz, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:ch1-qucosa-220086.

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We report an IgG (=immunoglobulin) electrochemical immunosensor using a newly synthesized redox-active ferrocenyl dendrimer of generation 2 (G2Fc) as a voltammetric transducer. The ferrocenyl dendrimer N(CH2CH2C(O)NHCH2CH2NHC(O)Fe(η5-C5H4)(η5-C5H5))(CH2CH2N(CH2CH2C(O)NHCH2CH2NHC(O)Fe(η5-C5H4)(η5-C5H5))2)2 (G2Fc) was used as a functional moiety to immobilize the antibody on the surface of the electrode. A sandwich immunosensor of the type IgG/Bovine serum albumin (BSA)/anti-IgG/G2Fc/glassy carbon electrode (GCE) was fabricated. The electrochemical properties of G2Fc were thoroughly studied in a

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32

Guilherme, Michel Silas. "In-orbit calibration of satellite drag free control systems." Aachen Shaker, 2007. http://d-nb.info/994071728/04.

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33

Neville, Rachel Frances. "Non-invasive measurement of oxidative stress in senior and exercising horses." Thesis, De Montfort University, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.275541.

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34

Ringwald, Matthias. "Reducing uncertainty in wireless sensor networks : network inspection and collision-free medium access /." Zürich : ETH, 2009. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=18209.

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35

Sivathasan, Sashigaran. "Hybrid radio frequency/free space optical communications for energy-efficient wireless sensor networks." Thesis, University of Oxford, 2008. http://ora.ox.ac.uk/objects/uuid:aee0f605-fb71-467b-9136-6816abaedcf1.

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A wireless sensor network (WSN) consists of a large number of networked sensor nodes deployed to sense and report a particular phenomenon to a base station. Currently, most WSNs use radio frequency (RF) communications, and this accounts for a significant amount of energy expended. Free space optical (FSO) communications using modulating retroreflectors is potentially attractive for WSNs, due to the lower communications energy required. However, for FSO communications, line of sight (LOS) is required between the transmitter and the receiver. In this thesis, a hybrid Radio Frequency/Free Space O

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36

Teratani, Mehrdad. "3D Image Processing and Communication in Camera Sensor Networks: Free Viewpoint Television Networking." Doctoral thesis, Nagoya University, Nagoya, Japan, 2004. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/319673.

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37

Botelho, Anthony. "Characterizing Productive Perseverance Using Sensor-Free Detectors of Student Knowledge, Behavior, and Affect." Digital WPI, 2019. https://digitalcommons.wpi.edu/etd-dissertations/523.

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Failure is a necessary step in the process of learning. For this reason, there has been a myriad of research dedicated to the study of student perseverance in the presence of failure, leading to several commonly-cited theories and frameworks to characterize productive and unproductive representations of the construct of persistence. While researchers are in agreement that it is important for students to persist when struggling to learn new material, there can be both positive and negative aspects of persistence. What is it, then, that separates productive from unproductive persistence? The pur

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38

CARDARELLI, STEFANO. "Optimal Filtering and Magnetometer-free Sensor Fusion: Applications to Human Motion Inertial Sensing." Doctoral thesis, Università Politecnica delle Marche, 2020. http://hdl.handle.net/11566/274521.

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Negli ultimi dieci anni, l'uso di sensoristica inerziale nell'ambito dell'analisi del movimento ha occupato un ruolo centrale nella letteratura scientifica relativamente agli ambiti di ingegneria elettronica e biomedica. La portabilità ed il basso costo di questo tipo di strumentazione hanno generanto un cresente interesse nello sviluppo di metodologie software per ottenere informazione accurata da dati inerziali e magnetici. Nel presente lavoro è riportato uno studio su comuni scenari di applicazione di tale strumentazione in analisi del movimento, considerando tuttavia misure puramente inerz

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39

Reuel, Nigel F. (Nigel Forest). "Label-free carbon nanotube sensors for glycan and protein detection." Thesis, Massachusetts Institute of Technology, 2013. http://hdl.handle.net/1721.1/89950.

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Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemical Engineering, June 2014.<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references.<br>Nanoengineered glycan sensors may help realize the long-held goal of accurate and rapid glycoprotein profiling without labeling or glycan liberation steps. Current methods of profiling oligosaccharides displayed on protein surfaces, such as liquid chromatography, mass spectrometry, capillary electrophoresis, and microarray methods, are limited by sample pretreatment and quantitative accuracy. Microarrayed platf

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FEARING, CHRISTOPHER JAMES. "IMAGING SENSORS WITH DATA COMMUNICATIONS CAPABILITIES." University of Cincinnati / OhioLINK, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1141352420.

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Scherer, Drew P. "Urban Free Agents: Active Territories Through Nascent Ubiquitous Networks." University of Cincinnati / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1336682944.

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Junfeng, Shi. "The lipid sensor Ffar4 regulates muscle-based diet-induced thermogenesis." 京都大学 (Kyoto University), 2017. http://hdl.handle.net/2433/225536.

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Högger, Andreas. "Dempster Shafer Sensor Fusion for Autonomously Driving Vehicles : Association Free Tracking of Dynamic Objects." Thesis, KTH, Skolan för elektro- och systemteknik (EES), 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-187814.

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Autonomous driving vehicles introduce challenging research areas combining diﬀer-ent disciplines. One challenge is the detection of obstacles with diﬀerent sensors and the combination of information to generate a comprehensive representation of the environment, which can be used for path planning and decision making.The sensor fusion is demonstrated using two Velodyne multi beam laser scanners, but it is possible to extend the proposed sensor fusion framework for diﬀerent sensor types. Sensor fusion methods are highly dependent on an accurate pose estimate, which can not be guaranteed in any c

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Duke, Connor R. "Optimization of control source and error sensor locations in free field active noise control /." Diss., CLICK HERE for online access, 2007. http://contentdm.lib.byu.edu/ETD/image/etd2087.pdf.

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Duke, Connor Raymond. "Optimization of Control Source and Error Sensor Locations in Free Field Active Noise Control." BYU ScholarsArchive, 2007. https://scholarsarchive.byu.edu/etd/1169.

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Previous work has shown that active noise control (ANC) can be applied to axial cooling fans. Optimization of the control source and error sensor placement is desired to maximize the attenuation using ANC. A genetic algorithm was developed to find the optimal placement of control sources for a given primary source. The optimal configuration of control sources around a single primary source was shown to be a linear arrangement of the sources. This holds true for both two-dimensional as well as three-dimensional configurations. The higher-order radiation of the linear arrangement has also been v

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46

Sitanayah, Lanny. "Finding boundary cycles in location-free low density wireless sensor networks for mobile target tracking." University of Western Australia. School of Computer Science and Software Engineering, 2009. http://theses.library.uwa.edu.au/adt-WU2009.0158.

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Wireless Sensor Networks (WSNs) comprise a large number of sensor nodes, which are spread out within a region and communicate using wireless links. In some WSN applications, recognizing boundary nodes is important for topology discovery, geographic routing and tracking. In this thesis, we study the problem of identifying the boundary nodes of a WSN. In a WSN, close-by nodes can communicate with their neighbors and have the ability to estimate distances to nearby nodes, but not necessarily the true distances. Our objective is to find the boundary nodes by using the connectivity relation and ne

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Nellore, Vishwa. "Mathematical Modeling and Experimental Verification of Resonance Energy Transfer Networks: Applications in Cryptography and Biological Sensing." Diss., 2014. http://hdl.handle.net/10161/9443.

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<p>FRET between pairs of fluorophores is widely used as a biological assay. However, the properties of larger fluorophore networks are poorly understood and their application space has not yet been fully explored. This dissertation introduces DNA self-assembled FÖrster Resonance Energy Transfer (FRET) networks that provide a unique optical output when probed by a series of light pulses. We create a Markov model of the FRET networks and analyze over 1200 time-resolved fluorescence measurements on 300 prototypical networks. Our results show that the optical responses of FRET networks are highly

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48

Tzu-HanLiang and 梁紫涵. "Integration of DNA Stretching and AC Electro-kinetics for Preparing a Robust Dynamic FRET Sensor." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/s359dp.

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49

Koudelková, Lenka. "Strukturní a regulační aspekty aktivace kinázy Src." Doctoral thesis, 2020. http://www.nusl.cz/ntk/nusl-435528.

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Src kinase plays a crucial role in a multitude of fundamental cellular processes. Src is an essential component of signalling pathways controlling cellular proliferation, motility or differentiation, and is often found deregulated in tumours. Src activity is therefore maintained under stringent and complex regulation mediated by SH3 and SH2 domains and the phosphorylation state of tyrosines 416 and 527. Active Src adopts an open conformation whereas inactive state of the kinase is characterised by a compact structure stabilised by inhibitory intramolecular interactions. We identified phosphory

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Casciato, Shelly Lynn 1984. "Development of a fluorescence model for the determination of constants associated with binding, quenching, and FRET efficiency and development of an immobilized FRET-peptide sensor for metal ion detection." Thesis, 2012. http://hdl.handle.net/2152/ETD-UT-2012-08-6087.

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Abstract:

This thesis presents a modeling program to obtain equilibrium information for a fluorescent system. Determining accurate dissociation constants for equilibrium processes involving a fluorescent mechanism can prove to be quite challenging. Typically, titration curves and non-linear least squares fitting of the data using computer programs are employed to obtain such constants. However, these approaches only consider the total fluorescence signal and often ignore other energy transfer processes within the system. The current model considers the impact on fluorescence from equilibrium binding

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