Journal articles: 'FSSC'

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De la Lastra, Eduardo, María Villarino, Juan Diego Astacio, Inmaculada Larena, Antonieta De Cal, and Nieves Capote. "Genetic Diversity and Vegetative Compatibility of Fusarium solani Species Complex of Strawberry in Spain." Phytopathology® 109, no. 12 (December 2019): 2142–51. http://dx.doi.org/10.1094/phyto-05-19-0173-r.

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Fusarium solani is a soilborne fungus that is a pathogen to >100 plant species. It is the causal agent of crown and root rot in strawberry. We collected 100 F. solani isolates from diseased plants and soils from two distinct geographic areas of strawberry production in Spain: plant nurseries located in the north-central region of the country and fruit production fields located in the southwestern region. The aims of this study were to accurately identify the isolates within the Fusarium solani species complex (FSSC) based on multilocus sequence typing, determine the genetic diversity and population structure of strawberry-associated FSSC based on phylogenetic analysis, and determine the vegetative compatibility among isolates in both strawberry production areas. Seven phylogenetic species, restricted to clade 3 of FSSC, were defined in the Spanish strawberry crops, showing a regional variation of species composition. Isolates from nurseries were composed of four phylogenetic species (i.e., FSSC 2, FSSC 5, FSSC 9, and an unknown FSSC species) that matched with five vegetative compatibility groups (VCGs). Isolates from fruit production fields included five phylogenetic species (i.e., FSSC 2, FSSC 3 + 4, FSSC 5, FSSC 6, and FSSC 11) distributed into 29 VCGs not correlated with phylogenetic groups. FSSC 5 and FSSC 2 were the most abundant species in nurseries and fruit production fields, respectively, and they were the only species present in both production areas. Of the 47 sequence-based haplotypes defined, no haplotypes were shared between nurseries and fruit production fields. Pathogenic isolates were present in all but FSSC 6 and FSSC 9 species, and FSSC 3 + 4 contained the higher percentage of pathogenic isolates. No relationship was observed between pathogenicity and the source of isolates (plant or soil). Generally, species present in fruit production fields showed higher genetic diversity than those present in nurseries. This work can contribute to understanding the diversity of this species complex in Spanish strawberry production areas, which will be useful for developing integrated disease management strategies.

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Chitrampalam, Periasamy, Nivi Abraham, and Berlin D. Nelson. "A Culture-Independent PCR-Based Assay to Detect the Root Rot Pathogen Fusarium solani Species Complex 11 from Soybean Roots and Soil." Plant Disease 102, no. 2 (February 2018): 327–33. http://dx.doi.org/10.1094/pdis-03-17-0447-re.

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Fusarium solani species complex (FSSC) 11 is the primary phylogenetic species of FSSC causing root rot in soybean in the north-central United States. A polymerase chain reaction (PCR)-based assay was developed to identify and differentiate FSSC 11 from the less aggressive FSSC 5 and other Fusarium and Pythium spp. associated with soybean roots. The primer set FSSC11-F and FSSC11-R designed from the RNA polymerase second largest subunit gene yielded the expected amplicon of about 900 bp with DNA from all 22 FSSC 11 isolates tested in PCR. However, it did not produce an amplicon with DNA from 29 isolates of FSSC 5, seven other Fusarium spp., three Pythium spp., and soybean tested in PCR. Furthermore, the primer set successfully detected FSSC 11 from a DNA mixture containing the DNA of FSSC 11, FSSC 5, other Fusarium spp., and soybean. The primer set also detected FSSC 11 from both soil and soybean roots. Additionally, the prevalence of FSSC 11 in soybean roots was determined in five fields in North Dakota by both a culture-independent PCR approach with FSSC11-F and FSSC11-R and a culture-dependent approach. Results from both the culture-dependent and culture-independent approaches with FSSC11-F and FSSC11-R were consistent and revealed the presence of the FSSC 11 in three of five fields sampled.

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Handaga, Bana, Tutut Herawan, and Mustafa Mat Deris. "FSSC." International Journal of Fuzzy System Applications 2, no. 4 (October 2012): 29–46. http://dx.doi.org/10.4018/ijfsa.2012100102.

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Introduced is a new algorithm for the classification of numerical data using the theory of fuzzy soft set, named Fuzzy Soft Set Classifier (FSSC). The algorithm uses the fuzzy approach in the pre-processing stage to obtain features, and similarity concept in the process of classification. It can be applied not only to binary-valued datasets, but also be able to classify the data that consists of real numbers. Comparison tests on seven datasets from UCI Machine Learning Repository have been carried out. It is shown that the proposed algorithm provides better accuracy and higher accuracy as compared to the baseline algorithm using soft set theory.

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Liu, Xuezhi. "Comparison on the Developmental Trends Between Chinese Students Studying Abroad and Foreign Students Studying in China." Journal of International Students 4, no. 1 (January 1, 2014): 34–47. http://dx.doi.org/10.32674/jis.v4i1.495.

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China has been undergoing a new period of political stability, cultural prosperity, and social harmony since its reform and opening-up in the late 1970s. At the same time, the number of Chinese students studying abroad (CSSA) and foreign students studying in China (FSSC) has grown rapidly and steadily in the past three decades. With China’s participation in globalization, CSSA and FSSC are a significant part of China’s potential international human capital and, as such, CSSA and FSSC are in great need. Data of CSSA and FSSC in the past 34 years are collected and examined in this article, and the historical trends are depicted and compared. Relationships between the developments of CSSA and FSSC and the development of China’s economy can be validated and compared by regression analysis. The number of CSSA and FSSC in the next 10 years can be predicted by predicting China’s GDP in the same period.

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Wang, Jie, Hyunkyu Sang, Janette L. Jacobs, Kjersten A. Oudman, Linda E. Hanson, and Martin I. Chilvers. "Soybean Sudden Death Syndrome Causal Agent Fusarium brasiliense Present in Michigan." Plant Disease 103, no. 6 (June 2019): 1234–43. http://dx.doi.org/10.1094/pdis-08-18-1332-re.

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Sudden death syndrome (SDS), caused by members of Fusarium solani species complex (FSSC) clade 2, is a major and economically important disease in soybean worldwide. The primary causal agent of SDS isolated to date in North America has been F. virguliforme. In 2014 and 2016, SDS symptoms were found in two soybean fields located on the same farm in Michigan. Seventy Fusarium strains were isolated from roots of the SDS-symptomatic soybeans in two fields. Phylogenetic analysis of partial sequences of elongation factor-1α, the nuclear ribosomal DNA intergenic spacer region, and the RNA polymerase II beta subunit revealed that the primary FSSC species isolated was F. brasiliense (58 and 36% in each field) and the remaining Fusarium strains were identified as F. cuneirostrum, F. phaseoli, an undescribed Fusarium sp. from FSSC clade 2, and strains in FSSC clade 5 and FSSC clade 11. Molecular identification was supported with morphological analysis and a pathogenicity assay. The soybean seedling pathogenicity assay indicated that F. brasiliense was capable of causing typical foliar SDS symptoms. Both root rot and foliar disease severity were variable by strain, just as they are in F. virguliforme. Both FSSC 5 and FSSC 11 strains were also capable of causing root rot, but SDS foliar symptoms were not detected. To our knowledge, this is the first report of F. brasiliense causing SDS in soybean in the United States and the first report of F. cuneirostrum, F. phaseoli, an as-yet-unnamed Fusarium sp., and strains in FSSC clade 5 and FSSC clade 11 associated with or causing root rot of soybean in Michigan.

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A Cordeiro, Rossana de, Fernando VM Portela, Lívia MG Pereira, Ana RC de Andrade, José K. de Sousa, Ana LR Aguiar, Mariana LM Pergentino, et al. "Efflux pump inhibition controls growth and enhances antifungal susceptibility of Fusarium solani species complex." Future Microbiology 15, no. 1 (January 2020): 9–20. http://dx.doi.org/10.2217/fmb-2019-0186.

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Aim: To evaluate the inhibition of efflux pumps by using promethazine (PMZ) as a strategy to control Fusarium solani species complex (FSSC). Materials & methods: The susceptibility of FSSC strains to PMZ and the interaction between PMZ and antifungals were evaluated. The efflux pump activity was confirmed by flow cytometry with rhodamine 6G. Finally, PMZ was tested against FSSC biofilms. Results: PMZ inhibited FSSC planktonic growth and showed synergism with antifungals. PMZ reduced R6G efflux and inhibited cell adhesion, impaired the development of biofilms and disrupted mature biofilms. PMZ-challenged biofilms showed increased sensitivity to amphotericin B. Conclusion: The study provides indirect evidence of the occurrence of efflux pumps in FSSC and opens a perspective for this target in the control of fusariosis.

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Xiao, Wei, Jing Huang, Wenjie Zhou, Qinglin Jiang, Ying Deng, Yanhua Zhang, and Liangliang Tian. "Surface Modification of Commercial Cotton Yarn as Electrode for Construction of Flexible Fiber-Shaped Supercapacitor." Coatings 11, no. 9 (September 8, 2021): 1086. http://dx.doi.org/10.3390/coatings11091086.

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In this study, we report on the rational design and facile preparation of a cotton-reduced graphene oxide-silver nanoparticle (cotton-RGO-AgNP) hybrid fiber as an electrode for the building of a flexible fiber-shaped supercapacitor (FSSC). It was adequately characterized and found to possess a well-defined core−shell structure with cotton yarn as a core and a porous RGO-AgNP coating as a shell. Thanks to the unique morphological features and low electrical resistance (only 2.3 Ω·cm−1), it displayed attractive supercapacitive properties. When evaluated in a three-electrode setup, this FSSC electrode delivered the highest linear and volumetric specific capacitance of up to ca. 12.09 mF·cm−1 and ca. 9.67 F·cm−3 with a satisfactory rate capability as well as a decent cycling stability. On the other hand, an individual parallel symmetric FSSC cell constructed by this composite fiber fulfilled the largest linear and volumetric specific capacitance of ca. 1.67 mF·cm−1 and ca. 0.67 F·cm−3 and offered the maximum energy density, as high as ca. 93.1 μWh·cm−3, which outperformed a great number of graphene- and textile yarn-based FSSCs. Impressively, bending deformation brought about quite a limited effect on its electrochemical behaviors and almost no capacitance degradation took place during the consecutive charge/discharge test for over 10,000 cycles. Consequently, these remarkable performances suggest that the currently developed cotton-RGO-AgNP fiber has considerable application potential in flexible, portable and wearable electronics.

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Purwanto, Agus, Leo Hutagalung, and Evy Yanthy. "FOOD SAFETY MANAGEMENT LEADERSHIP STYLE: TRANSFORMATIONAL OR TRANSACTIONAL?" Jurnal Ekonomi dan Manajemen 14, no. 1 (January 29, 2020): 170–82. http://dx.doi.org/10.30650/jem.v14i1.1288.

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The purpose of this study was to analyze the effect of transformational and transactional leadership style on the performance of the latest version of the FSSC 22000 (Food Safety System Certification) food safety management system, version 5 of 2019 in the food industry in Tangerang. The object of the research were employees in several food industries that worked in the Tangerang City area who had implemented FSSC 22000 version 5 2019; as many as 120 people, and data collection method was done by giving out electronic questionnaire to several employees randomly. The analysis tool in this study used SEM (Structural Equation Model) LISREL program version 8.70. The results showed that the transformational leadership style positively and significantly affected the performance of the FSSC 22000 implementation and the transactional leadership style also contributed positive effect on the performance of the FSSC 22000 food safety management system and it can be concluded that leadership was important for the implementation of the FSSC 22000 Food Safety Management System.

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Badali, Hamid, Hoja Patterson, Carmita Sanders, Barbara Mermella, Connie Gibas, James Mele, Hongxin Fan, Ashraf S. Ibrahim, Karen J. Shaw, and Nathan P. Wiederhold. "1282. Manogepix, the Active Moiety of the Investigational Agent Fosmanogepix, Demonstrates In vitro Activity Against Members of the Fusarium oxysporum and Fusarium solani Species Complexes." Open Forum Infectious Diseases 7, Supplement_1 (October 1, 2020): S656. http://dx.doi.org/10.1093/ofid/ofaa439.1465.

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Abstract Background Invasive fusariosis is associated with marked morbidity and mortality in immunocompromised hosts, and treatment options are limited. Common etiologic agents include members of the F. oxysporum and F. solani species complexes (FOSC and FSSC, respectively). Manogepix (MGX), the active moiety of fosmanogepix, is a novel GWT1 inhibitor with broad antifungal activity. Fosmanogepix has previously shown in vivo efficacy in an immunocompromised murine model of invasive fusariosis. Our objective was to evaluate the in vitro activity of MGX against FOSC and FSSC isolates. Methods Clinical isolates of FOSC (n=49) and FSSC (19) were identified by combined phenotypic characteristics and DNA sequence analysis of the translation elongation factor 1-alpha (TEF1α) and RNA polymerase II second largest subunit (RPB2). Antifungal susceptibility testing was performed by CLSI M38 broth microdilution. Minimum effective concentrations (MEC) and minimum inhibitory concentrations (MIC) were read after 48 hours of incubation at 50% and 100% inhibition of growth for MGX, and MIC values were read for amphotericin B, posaconazole, isavuconazole, and voriconazole at 100% inhibition of growth. Results MGX demonstrated potent in vitro activity against both FOSC and FSSC isolates. Against the 49 FOSC isolates, the MGX MECs ranged from <0.015-0.03 mg/mL, and MICs at the 50% inhibition of growth endpoint ranged from <0.015-0.12 mg/mL (Table). MIC values were higher when read at 100% inhibition of growth. Similar results were observed against FSSC isolates (MEC and MIC ranges <0.015 and <0.015-0.25 mg/mL, respectively). MGX MEC and MIC 50% inhibition values were in close agreement for both FOSC and FSSC isolates. Of the other antifungals tested, amphotericin B demonstrated in vitro good activity (MIC ranges 1-4 and 0.25-4 mg/mL against FOSC and FSSC, respectively). In contrast, the azoles demonstrated reduced susceptibility (MIC range 1- >16 mg/mL). MIC/MEC values (mcg/mL) for manogepix and other antifungals against FOSC and FSSC isolates Conclusion MGX demonstrated in vitro activity against FOSC and FSSC clinical isolates. Both changes in fungal morphology (MEC) and reductions in growth (MIC 50% inhibition) were observed. Clinical studies are ongoing to determine the efficacy of fosmanogepix in patients with invasive fungal infections. Disclosures Ashraf S. Ibrahim, PhD, Astellas Pharma (Research Grant or Support) Karen J. Shaw, PhD, Amplyx (Consultant)Forge Therapeutics (Consultant) Nathan P. Wiederhold, PharmD, Astellas (Grant/Research Support)BioMerieux (Grant/Research Support)Cepheid (Grant/Research Support)Covance (Grant/Research Support)F2G (Grant/Research Support)Gilead (Speaker’s Bureau)Mayne Pharma (Advisor or Review Panel member)Sfunga (Grant/Research Support)

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Chehri, Khosrow. "Molecular phylogeny of the Fusarium solani species complex (FSSC) isolated from soils in Iran." Botany 92, no. 11 (November 2014): 815–20. http://dx.doi.org/10.1139/cjb-2014-0096.

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Members of Fusarium solani species complex (FSSC) are frequently isolated from soils, food, feeds, trees, and to some extent from humans and other animals. The taxonomic status of these fungi is being revised but no attempt has been made to identify those isolated in Iran, a mountainous country with a high biodiversity. The objective of the present research was to study the phylogenetic diversity of FSSC strains recovered from soils in Iran by analyzing morphological characteristics and DNA sequences. A total of 65 strains belonging to the FSSC were recovered from agricultural soils in western Iran. Based on differences in their morphological characters, 25 strains were selected for phylogenetic analysis employing translation elongation factor-1α (tef1) and internal transcribed spacer (ITS) region sequences. Comparisons of DNA sequence data revealed that all isolates belonged to Fusarium falciforme, Fusarium keratoplasticum, Fusarium petroliphilum, the unnamed species FSSC 5, and unknown species of Fusarium, which represents a new lineage within members of Clade 3. Based on morphological features and phylogenetic study, F. keratoplasticum and F. petroliphilum were reported for the first time in Iran.

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O'Donnell, Kerry, Deanna A. Sutton, Nathan Wiederhold, Vincent A. R. G. Robert, Pedro W. Crous, and David M. Geiser. "Veterinary Fusarioses within the United States." Journal of Clinical Microbiology 54, no. 11 (September 7, 2016): 2813–19. http://dx.doi.org/10.1128/jcm.01607-16.

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Multilocus DNA sequence data were used to assess the genetic diversity and evolutionary relationships of 67Fusariumstrains from veterinary sources, most of which were from the United States. Molecular phylogenetic analyses revealed that the strains comprised 23 phylogenetically distinct species, all but two of which were previously known to infect humans, distributed among eight species complexes. The majority of the veterinary isolates (47/67 = 70.1%) were nested within theFusarium solanispecies complex (FSSC), and these included 8 phylospecies and 33 unique 3-locus sequence types (STs). Three of the FSSC species (Fusarium falciforme,Fusarium keratoplasticum, andFusariumsp. FSSC 12) accounted for four-fifths of the veterinary strains (38/47) and STs (27/33) within this clade. Most of theF. falciformestrains (12/15) were recovered from equine keratitis infections; however, strains ofF. keratoplasticumandFusariumsp. FSSC 12 were mostly (25/27) isolated from marine vertebrates and invertebrates. Our sampling suggests that theFusarium incarnatum-equisetispecies complex (FIESC), with eight mycoses-associated species, may represent the second most important clade of veterinary relevance withinFusarium. Six of the multilocus STs within the FSSC (3+4-eee, 1-b, 12-a, 12-b, 12-f, and 12-h) and one each within the FIESC (1-a) and theFusarium oxysporumspecies complex (ST-33) were widespread geographically, including three STs with transoceanic disjunctions. In conclusion, fusaria associated with veterinary mycoses are phylogenetically diverse and typically can only be identified to the species level using DNA sequence data from portions of one or more informative genes.

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Takashina, Kuya, Izumi Chuma, Hisashi Kajimura, Norikazu Kameyama, Chiaki Goto, and Keiko Kuroda. "Pathogenicity and Distribution of Fusarium solani Isolates Associated with Erythrina Decline in Japan." Plant Disease 104, no. 3 (March 2020): 731–42. http://dx.doi.org/10.1094/pdis-01-19-0044-re.

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Erythrina spp. trees have been declining since the 2000s worldwide, and fungi belonging to Fusarium solani species complex (FSSC) have been suggested to be a causal factor of decline and mortality of Erythrina variegata trees in Okinawa Island, Japan. In addition to the FSSC isolate grouped as “it-1” based on ITS sequence data (previously called strain A), we conducted an inoculation experiment with two isolates grouped as “it-2” (previously strain B), which is genetically close to it-1. Two it-2 isolates originating from two islands showed pathogenicity to E. variegata with the same symptoms as those caused by it-1 isolate. We also found the isolates of it-1 and it-2 were widely distributed, including on Ishigaki Island, ∼400 km south of Okinawa Island across the ocean. All isolates of it-1 and it-2 belong to the ambrosia Fusarium clade of the FSSC, a group of symbionts of ambrosia beetles, including the pathogens of Fusarium dieback in avocados and teas. The detection of ambrosia beetles Euwallacea spp. from our specimens provided information on the vectors of the pathogens. Our present results suggest the fungi of the FSSC could be responsible for the Erythrina decline in other areas with damage.

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Mohamed Zubi, Wafa S., Masratul Hawa Mohd, Nik Mohd Izham Mohamed Nor, and Latiffah Zakaria. "Fusarium Species in Mangrove Soil in Northern Peninsular Malaysia and the Soil Physico-Chemical Properties." Microorganisms 9, no. 3 (February 26, 2021): 497. http://dx.doi.org/10.3390/microorganisms9030497.

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Fusarium genus comprises important saprophytic and phytopathogenic fungi and is widespread in nature. The present study reports the occurrence of Fusarium spp. in soils from two mangrove forests in northern Peninsular Malaysia and analyzed physico-chemical properties of the mangrove soil. Based on TEF-1α sequences, nine Fusarium species were identified: Fusarium solani species complex (FSSC) (n = 77), Fusarium verticillioides (n = 20), Fusarium incarnatum (n = 10), Fusarium proliferatum (n = 7), Fusarium lateritium (n = 4), Fusarium oxysporum (n = 3), Fusarium rigidiuscula (n = 2), Fusarium chlamydosporum (n = 1), and Fusarium camptoceras (n = 1); FSSC isolates were the most prevalent. Phylogenetic analysis of the combined TEF-1α and ITS sequences revealed diverse phylogenetic affinities among the FSSC isolates and potentially new phylogenetic clades of FSSC. Soil analysis showed varied carbon content, pH, soil moisture, and salinity, but not nitrogen content, between sampling locations. Regardless of the physico-chemical properties, various Fusarium species were recovered from the mangrove soils. These were likely saprophytes; however, some were well-known plant pathogens and opportunistic human pathogens. Thus, mangrove soils might serve as inoculum sources for plant and human pathogenic Fusarium species. The present study demonstrates the occurrence of various Fusarium species in the extreme environment of mangrove soil, thereby contributing to the knowledge on species diversity in Fusarium.

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Walther, Grit, Serena Stasch, Kerstin Kaerger, Axel Hamprecht, Mathias Roth, Oliver A. Cornely, Gerd Geerling, Colin R. Mackenzie, Oliver Kurzai, and Marie von Lilienfeld-Toal. "Fusarium Keratitis in Germany." Journal of Clinical Microbiology 55, no. 10 (July 26, 2017): 2983–95. http://dx.doi.org/10.1128/jcm.00649-17.

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ABSTRACT Fusarium keratitis is a destructive eye infection that is difficult to treat and results in poor outcome. In tropical and subtropical areas, the infection is relatively common and associated with trauma or chronic eye diseases. However, in recent years, an increased incidence has been reported in temperate climate regions. At the German National Reference Center, we have observed a steady increase in case numbers since 2014. Here, we present the first German case series of eye infections with Fusarium species. We identified Fusarium isolates from the eye or eye-related material from 22 patients in 2014 and 2015. Thirteen isolates belonged to the Fusarium solani species complex (FSSC), 6 isolates belonged to the Fusarium oxysporum species complex (FOSC), and three isolates belonged to the Fusarium fujikuroi species complex (FFSC). FSSC was isolated in 13 of 15 (85%) definite infections and FOSC in 3 of 4 (75%) definite contaminations. Furthermore, diagnosis from contact lens swabs or a culture of contact lens solution turned out to be highly unreliable. FSSC isolates differed from FOSC and FFSC by a distinctly higher MIC for terbinafine. Outcome was often adverse, with 10 patients requiring keratoplasty or enucleation. The use of natamycin as the most effective agent against keratitis caused by filamentous fungi was rare in Germany, possibly due to restricted availability. Keratitis caused by Fusarium spp. (usually FSSC) appears to be a relevant clinical problem in Germany, with the use of contact lenses as the predominant risk factor. Its outcome is often adverse.

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Botelho, Natercio José. "A implantação do modelo de segurança de alimentos FSSC 22000 nos processos de uma refinaria de açúcar." Revista de Engenharia e Pesquisa Aplicada 4, no. 3 (April 29, 2019): 32–39. http://dx.doi.org/10.25286/repa.v4i3.984.

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Para uma produção segura de alimentos, podem ser utilizadas ferramentas de qualidade no processo produtivo ou também atividades de controle de inspeção no produto final. No entanto, é importante que a empresa tenha não apenas ações pontuais de melhoria, mas um modelo de gestão que proporcione o cumprimento de procedimentos e proporcione a melhoria de forma sistemática. Este artigo tem por objetivo demonstrar a importância do esquema FSSC 22000(modelo para gestão de segurança de alimentos) para a garantia da segurança dos processos em uma indústria de refino de açúcar. Para essa finalidade, foi realizada uma revisão de literatura, com base em estudo de caso, por meio de pesquisa exploratória e visitas técnicas em uma refinaria de uma indústria sucroalcooleira, que já tem implantado o plano para análise de perigos e pontos críticos de controle (APPCC) e a norma ABNT NBR ISO 9001, mas optou pela certificação no modelo FSSC 22000. Foi possível observar a eficácia do modelo FSSC 22000 na garantia da segurança dos produtos da indústria sucroalcooleira, proporcionando melhores resultados ao sistema de gestão da organização, mesmo com as iniciativas de qualidade já existentes.

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Roth, Mitchell G., Kjersten A. Oudman, Amanda Griffin, Janette L. Jacobs, Hyunkyu Sang, and Martin I. Chilvers. "Diagnostic qPCR Assay to Detect Fusarium brasiliense, a Causal Agent of Soybean Sudden Death Syndrome and Root Rot of Dry Bean." Plant Disease 104, no. 1 (January 2020): 246–54. http://dx.doi.org/10.1094/pdis-01-19-0016-re.

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Species within clade 2 of the Fusarium solani species complex (FSSC) are significant pathogens of dry bean (Phaseolus vulgaris) and soybean (Glycine max), causing root rot and/or sudden death syndrome (SDS). These species are morphologically difficult to distinguish and often require molecular tools for proper diagnosis to a species level. Here, a TaqMan probe-based quantitative PCR (qPCR) assay was developed to distinguish Fusarium brasiliense from other closely related species within clade 2 of the FSSC. The assay displays high specificity against close relatives and high sensitivity, with a detection limit of 100 fg. This assay was able to detect F. brasiliense from purified mycelia, infected dry bean roots, and soil samples throughout Michigan. When multiplexed with an existing qPCR assay specific to Fusarium virguliforme, accurate quantification of both F. brasiliense and F. virguliforme was obtained, which can facilitate accurate diagnoses and identify coinfections with a single reaction. The assay is compatible with multiple qPCR thermal cycling platforms and will be helpful in providing accurate detection of F. brasiliense. Management of root rot and SDS pathogens in clade 2 of the FSSC is challenging and must be done proactively, because no midseason management strategies currently exist. However, accurate detection can facilitate management decisions for subsequent growing seasons to successfully manage these pathogens.

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Burkhardt, Käthe, Helene Loxton, and Peter Muris. "Fears and Fearfulness in South-African Children." Behaviour Change 20, no. 2 (June 1, 2003): 94–102. http://dx.doi.org/10.1375/bech.20.2.94.24837.

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AbstractThe present study examined common childhood fears in 9- to 13-year-old South-African children (N = 404) from white, coloured, and black cultural groups. Fears were assessed by means of two methods — the fear list method and the Fear Survey Schedule for Children-Revised (FSSC-R). Results showed that fear rank orders as obtained with the fear list method were quite different from those derived from the FSSC-R. Furthermore, clear differences in fear levels were found among the three cultural groups. More specifically, coloured and black South-African children displayed significantly higher fear levels than white children. Finally, differences were also found as to the content of prevalent fears in the three cultural groups. For example, common fears in coloured and black children were more frequently related to violence than in white children.

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Gilmore, Linda, and Marilyn Campbell. "Spiders, bullies, monsters or terrorists: What scares Australian children?" Children Australia 32, no. 3 (2007): 29–33. http://dx.doi.org/10.1017/s1035077200011640.

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In recent times, Australian children have been exposed to a range of frightening images of war and terrorism in the media. To determine the possible impact of such distal events, fears were measured in a sample of 220 children aged 6 to 12 years using the Fear Survey Schedule for Children (FSSC-R) as well as a free option method. On the FSSC-R, the type and intensity of children’s fears were similar to previous studies conducted over the past two decades, with being hit by a car, bombs and being unable to breathe producing the most fear. By contrast, spontaneous responses indicated that children’s greatest fears were of animals, the dark and being lost. Surprisingly few children mentioned war and terrorism without prompting. The findings suggest that concerns about Australian children becoming more fearful as a result of media coverage of war and terrorism are not supported.

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Zhang, Ning, David M. Geiser, and Christine D. Smart. "Macroarray Detection of Solanaceous Plant Pathogens in the Fusarium solani Species Complex." Plant Disease 91, no. 12 (December 2007): 1612–20. http://dx.doi.org/10.1094/pdis-91-12-1612.

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Members of the Fusarium solani species complex (FSSC), which are morphologically similar but have more than 45 distinct lineages, were chosen as targets for the development of a macroarray detection system that would be broadly adaptable. Thirty-three oligonucleotides (17 to 27 mers) were designed from the internal transcribed spacer (ITS) of the ribosomal RNA genes of 17 FSSC isolates, which belong to 12 phylogenetically closely related species. Of the 33 oligonucleotides on the array, 21 were useful in discriminating all 12 species, some of which had only a single nucleotide difference among them. The high specificity of this method was achieved by optimizing the hybridization temperature and oligo probe length, which had a more substantial effect on the array performance than the melting temperature and the DNA G+C content (G/C%) of the probes. The array was validated by testing inoculated greenhouse samples and diseased field plant samples.

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Lightfoot, Jorge D., and Kevin K. Fuller. "CRISPR/Cas9-Mediated Gene Replacement in the Fungal Keratitis Pathogen Fusarium solani var. petroliphilum." Microorganisms 7, no. 10 (October 16, 2019): 457. http://dx.doi.org/10.3390/microorganisms7100457.

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Fungal keratitis (FK) is a site-threatening infection of the cornea associated with ocular trauma and contact lens wear. Members of the Fusarium solani species complex (FSSC) are predominant agents of FK worldwide, but genes that support their corneal virulence are poorly understood. As a means to bolster genetic analysis in FSSC pathogens, we sought to employ a CRISPR/Cas9 system in an FK isolate identified as Fusarium petroliphilum. Briefly, this approach involves the introduction of two components into fungal protoplasts: (1) A purified Cas9 protein complexed with guide RNAs that will direct the ribonuclease to cut on either side of the gene of interest, and (2) a “repair template” comprised of a hygromycin resistance cassette flanked by 40 bp of homology outside of the Cas9 cuts. In this way, Cas9-induced double strand breaks should potentiate double homologous replacement of the repair template at the desired locus. We targeted a putative ura3 ortholog since its deletion would result in an easily discernable uracil auxotrophy. Indeed, 10% of hygromycin-resistant transformants displayed the auxotrophic phenotype, all of which harbored the expected ura3 gene deletion. By contrast, none of the transformants from the repair template control (i.e., no Cas9) displayed the auxotrophic phenotype, indicating that Cas9 cutting was indeed required to promote homologous integration. Taken together, these data demonstrate that the in vitro Cas9 system is an easy and efficient approach for reverse genetics in FSSC organisms, including clinical isolates, which should enhance virulence research in these important but understudied ocular pathogens.

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Ramani, Rama, and Vishnu Chaturvedi. "Evaluations of Shorter Exposures of Contact Lens Cleaning Solutions against Fusarium oxysporum Species Complex and Fusarium solani Species Complex To Simulate Inappropriate Usage." Antimicrobial Agents and Chemotherapy 55, no. 5 (February 7, 2011): 2265–75. http://dx.doi.org/10.1128/aac.00634-09.

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ABSTRACTAn outbreak ofFusariumkeratitis in contact lens users resulted in withdrawal of ReNu with MoistureLoc solution, although the exact cause of the outbreak remains enigmatic. We evaluated current and discontinued multipurpose cleaning solutions (MPSs; MoistureLoc, Equate, MultiPlus, and OptiFree Express) against plankton- and biofilm-derived cells ofFusarium oxysporumspecies complex (FOSC) andF. solanispecies complex (FSSC). The methods included a traditional assay based on CFU counts and a novel flow cytometry (FC) assay based on percent cell subpopulation (PCS) stained with two fluorochromes (Sytox Red and 5-chloromethylfluorescein diacetate). The tests were done with the respective manufacturers' recommended cleaning regimens (240 to 360 min) and under shorter exposures (15 to 60 min) to simulate inappropriate usage by the customers. FC assay measured PCS, which was available rapidly, in 5 to 7 h, whereas 24 to 48 h was needed for CFU counts, and there was good correlation between the two methods (r2= 0.97). FC assays allowed identification of injured fungal cells, which are likely to be missed with growth assays. In general, a time- and inoculum-dependent survival pattern was seen for both FOSC and FSSC cells, and biofilm-derived cells were more resistant than plankton-derived cells. MultiPlus and Equate produced 100% sterilization of fungi even under shorter exposures. However, biofilm FOSC and FSSC cells survived for up to 4 h in MoistureLoc solution and up to 6 h in OptiFree Express solution under shorter exposure times. This finding was enigmatic, as OptiFree Express is not associated with any outbreak ofFusariumkeratitis. This study provides additional support for possible roles that improper lens cleaning regimens and fungal biofilms could play as predisposing factors forFusariumkeratitis.

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Kuzlyakina, Yu A., E. V. Kryuchenko, and Z. A. Yurchak. "Overview of the new version of FSSC 22000 (v.5)." Vsyo o myase, no. 5 (October 31, 2019): 3–5. http://dx.doi.org/10.21323/2071-2499-2019-5-3-5.

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Hui, Jia, Daoxin Wei, Jing Chen, and Zhou Yang. "Polyaniline Nanotubes/Carbon Cloth Composite Electrode by Thermal Acid Doping for High-Performance Supercapacitors." Polymers 11, no. 12 (December 11, 2019): 2053. http://dx.doi.org/10.3390/polym11122053.

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Carbon materials have been widely used in designing supercapacitors (SCs) but the capacitance is not ideal. Herein, we synthesize polyaniline (PANI) nanotubes on the basis of a carbon cloth (CC) through a one-step self-degradation template method, and fabricate a CC@PANI NTs-H (CC@PANI nanotubes doping at high temperature) composite electrode by thermal acid doping. The CC@PANI NTs-H electrode obviously exhibits better electrochemical performance with a gravimetric capacitance of 438 F g−1 and maintains 86.8% after 10,000 cycles than the CC@PANI NTs-R (CC@PANI nanotubes doping at room temperature) electrode. Furthermore, we assemble a flexible solid state supercapacitor (FSSC) device with the as-prepared CC@PANI NTs-H composite electrodes, showing good flexibility and outstanding electrochemical performances with a high gravimetric capacitance of 247 F g−1, a large energy density of 21.9 Wh kg−1, and a capacitance retention of 85.4% after 10,000 charge and discharge cycles. Our work proposes a novel and easy pathway to fabricate low-cost FSSCs for the development of energy storage devices.

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Chang, Xiaoli, Li Yan, Muhammd Naeem, Muhammad Ibrahim Khaskheli, Hao Zhang, Guoshu Gong, Min Zhang, et al. "Maize/Soybean Relay Strip Intercropping Reduces the Occurrence of Fusarium Root Rot and Changes the Diversity of the Pathogenic Fusarium Species." Pathogens 9, no. 3 (March 13, 2020): 211. http://dx.doi.org/10.3390/pathogens9030211.

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Fusarium species are the most detrimental pathogens of soybean root rot worldwide, causing large loss in soybean production. Maize/soybean relay strip intercropping has significant advantages on the increase of crop yields and efficient use of agricultural resources, but its effects on the occurrence and pathogen population of soybean root rot are rarely known. In this study, root rot was investigated in the fields of the continuous maize/soybean strip relay intercropping and soybean monoculture. Fusarium species were isolated from diseased soybean roots and identified based on sequence analysis of translation elongation factor 1α (EF-1α) and RNA polymerase II second largest subunit (RPB2), and the diversity and pathogenicity of these species were also analyzed. Our results showed that intercropping significantly decreased soybean root rot over monoculture. A more diverse Fusarium population including Fusarium solani species complex (FSSC), F. incarnatum-equiseti species complex (FIESC), F. oxysporum, F. fujikuroi, F. proliferatum and F. verticillioides, F. graminearum and F. asiaticum was identified from intercropping while FSSC, FIESC, F. oxysporum, F. commune, F. asiaticum and F. meridionale were found from monoculture. All Fusarium species caused soybean root infection but exhibited distinct aggressiveness. The most aggressive F. oxysporum was more frequently isolated in monoculture than intercropping. FSSC and FIESC were the dominant species complex and differed in their aggressiveness. Additionally, F. fujikuroi, F. proliferatum and F. verticillioides were specifically identified from intercropping with weak or middle aggressiveness. Except for F. graminearum, F. meridionale and F. asiaticum were firstly reported to cause soybean root rot in China. This study indicates maize/soybean relay strip intercropping can reduce soybean root rot, change the diversity and aggressiveness of Fusarium species, which provides an important reference for effective management of this disease.

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Guo, Jifa, Shihong Du, Hongyuan Huo, Shouji Du, and Xiuyuan Zhang. "Modelling the Spectral Uncertainty of Geographic Features in High-Resolution Remote Sensing Images: Semi-Supervising and Weighted Interval Type-2 Fuzzy C-Means Clustering." Remote Sensing 11, no. 15 (July 25, 2019): 1750. http://dx.doi.org/10.3390/rs11151750.

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The spectral uncertainty refers to the diversity and variations of spectral characteristics within a single geographic object or across different objects of the same class. Usually, existing methods represent the spectral characteristics as precise single-valued curves. Thus, the spectral variations cannot be modeled, which further restricts the analysis and classification performance of remote sensing images. On the other hand, unsupervised methods have poor performance in classification and modeling uncertainty, while supervised methods need a large number of samples with high quality. Fuzzy semi-supervised clustering (FSSC) methods achieve a high accuracy with limited labelled samples. Thus, currently, FSSC methods attract more and more attention. This paper proposes a novel method to model the spectral uncertainty for very-high-resolution (VHR) images based on interval type-2 fuzzy sets (IT2 FSs), namely the hierarchical semi-supervising and weighted interval type-2 fuzzy c-means for objects (hierarchical SSW-IT2FCM-O) clustering method. In this method, the VHR image is segmented into image objects to reduce spectral uncertainty within objects. Spectral values, spectral indices and textures were weighted for object-based image classification. To further reduce spectral uncertainty across different objects of the same class, the spectral characteristics of land cover types were represented as banded curves with certain widths instead of precise single-valued spectral curves. The experimental results show that the banded spectral curves produced by the hierarchical SSW-IT2FCM-O can effectively model the spectral uncertainty of geographic objects. From the perspective of classification, four typical validity indices along with the confusion matrix and kappa coefficient were used to test the effectiveness of the hierarchical SSW-IT2FCM-O method, and these indices show that the presented method SSW-IT2FCM-O has greater classification accuracy than the existing FSSC methods and, more importantly, it requires smaller training samples than the existing methods.

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Cantanhede, Vanessa, Karen Signori Pereira, and Daniel Weingart Barreto. "FSSC 22000 Packaging Implementation: a Plastics Industry Research." Polímeros 28, no. 1 (March 15, 2018): 38–43. http://dx.doi.org/10.1590/0104-1428.06816.

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Baurina, S. B., and R. I. Amirova. "FSSC 22000 Certification as a Food Security Tool." IOP Conference Series: Earth and Environmental Science 666, no. 3 (March 1, 2021): 032060. http://dx.doi.org/10.1088/1755-1315/666/3/032060.

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Chitrampalam, P., and B. Nelson. "Multilocus phylogeny reveals an association of agriculturally important Fusarium solani species complex (FSSC) 11, and clinically important FSSC 5 and FSSC 3 + 4 with soybean roots in the north central United States." Antonie van Leeuwenhoek 109, no. 2 (December 15, 2015): 335–47. http://dx.doi.org/10.1007/s10482-015-0636-7.

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Bouvard, Martine, Anne Denis, and Jean-Luc Roulin. "The French Version of the Revised Child Anxiety and Depression Scale (RCADS) in a Nonclinical Sample." Swiss Journal of Psychology 74, no. 3 (June 18, 2015): 119–27. http://dx.doi.org/10.1024/1421-0185/a000158.

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This article investigates the psychometric properties of the Revised Child Anxiety and Depression Scale (RCADS). A group of 704 adolescents completed the questionnaires in their classrooms. This study examines potential confirmatory factor analysis factor models of the RCADS as well as the relationships between the RCADS and the Screen for Child Anxiety Related Emotional Disorders-Revised (SCARED-R). A subsample of 595 adolescents also completed an anxiety questionnaire (Fear Survey Schedule for Children-Revised, FSSC-R) and a depression questionnaire (Center for Epidemiological Studies Depression Scale, CES-D). Confirmatory factor analysis of the RCADS suggests that the 6-factor model reasonably fits the data. All subscales were positively intercorrelated, with rs varying between .48 (generalized anxiety disorder-major depression disorder) and .65 (generalized anxiety disorder-social phobia/obsessive-compulsive disorder). The RCADS total score and all the RCADS scales were found to have good internal consistency (> .70). The correlations between the RCADS subscales and their SCARED-R counterparts are generally substantial. Convergent validity was found with the FSSC-R and the CES-D. The study included normal adolescents aged 10 to 19. Therefore, the findings cannot be extended to children under 10, nor to a clinical population. Altogether, the French version of the RCADS showed reasonable psychometric properties.

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Shore, Gail N., and Mark D. Rapport. "The Fear Survey Schedule for Children-Revised (FSSC-HI)." Journal of Anxiety Disorders 12, no. 5 (September 1998): 437–61. http://dx.doi.org/10.1016/s0887-6185(98)00027-9.

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Sillanpää, Jouko, Sreedhar R. Nallapareddy, Janeu Houston, Vannakambadi K. Ganesh, Agathe Bourgogne, Kavindra V. Singh, Barbara E. Murray, and Magnus Höök. "A family of fibrinogen-binding MSCRAMMs from Enterococcus faecalis." Microbiology 155, no. 7 (July 1, 2009): 2390–400. http://dx.doi.org/10.1099/mic.0.027821-0.

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We report that three (EF0089, EF2505 and EF1896, renamed here Fss1, Fss2 and Fss3, respectively, for Enterococcus faecalis surface protein) of the recently predicted MSCRAMMs (microbial surface components recognizing adhesive matrix molecules) in E. faecalis strain V583 bind fibrinogen (Fg). Despite an absence of extensive primary sequence homology, the three proteins appear to be related structurally. Within the N-terminal regions of the three enterococcal proteins, we identified pairs of putative IgG-like modules with a high degree of predicted structural similarity to the Fg-binding N2 and N3 domains of the staphylococcal MSCRAMMs ClfA and SdrG. A second N2N3-like segment was predicted in Fss1. Far-UV circular dichroism spectroscopy revealed that all four predicted N2N3-like regions are composed mainly of β-sheets with only a minor proportion of α-helices, which is characteristic of Ig-like folded domains. Three of the four identified enterococcal N2N3-like regions showed potent dose-dependent binding to Fg. However, the specificity of the Fg-binding MSCRAMMs differs, as indicated by far-Western blots, which showed that recombinant segments of the MSCRAMMs bound different Fg polypeptide chains. Enterococci grown in serum-supplemented broth adhere to Fg-coated surfaces, and inactivation in strain OG1RF of the gene encoding Fss2 resulted in reduced adherence, whilst complementation of the mutant restored full Fg adherence. Thus, E. faecalis contains a family of MSCRAMMs that structurally and functionally resemble the Fg-binding MSCRAMMs of staphylococci.

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Cafarchia, Claudia, Romina Paradies, Luciana A. Figueredo, Roberta Iatta, Salvatore Desantis, Antonio Vito Francesco Di Bello, Nicola Zizzo, and Anne D. van Diepeningen. "Fusarium spp. in Loggerhead Sea Turtles (Caretta caretta): From Colonization to Infection." Veterinary Pathology 57, no. 1 (October 29, 2019): 139–46. http://dx.doi.org/10.1177/0300985819880347.

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With the aim of evaluating the presence of Fusarium spp. in sea turtles with and without lesions and assessing the risk factors favoring colonization and/or infection, 74 loggerhead sea turtles ( Caretta caretta) admitted to rescue and rehabilitation clinics in Italy were analyzed. The study compared 31 individuals with no apparent macroscopic lesions and 43 individuals with macroscopic lesions. Shell and skin samples were analyzed using Calcofluor white with 10% potassium hydroxide, standard histopathological examination, and fungal cultures. Fusarium spp. were isolated more frequently from animals with superficial lesions (39%) than from those with no macroscopic lesions (16%). Isolates from animals with superficial lesions were Fusarium solani species complex (FSSC) lineages haplotypes 9, 12, and 27 (unnamed lineages), FSSC-2 ( Fusarium keratoplasticum), Fusarium oxysporum (27%), and Fusarium brachygibbosum (3%). In contrast, only F. solani haplotypes 9 and 12 were isolated from animals with no macroscopic lesions. The presence of lesions was identified as a risk factor for the occurrence of Fusarium spp. Of the 74 animals, only 7 (9.5%) scored positive on microscopic examination with Calcofluor, and histological examination of those 7 animals revealed necrosis, inflammatory cells, and fungal hyphae in the carapace and skin. The results of this study suggest that fusariosis should be included in the differential diagnosis of shell and skin lesions in sea turtles. Direct examination using Calcofluor and potassium hydroxide was not useful to diagnose the infection. Histopathological examination and fungal culture should be performed to ensure correct treatment and infection control.

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Madhu, Swati N., Savitri Sharma, and Devarshi U. Gajjar. "Identification of Proteases: Carboxypeptidase and Aminopeptidase as Putative Virulence Factors of Fusarium solani Species Complex." Open Microbiology Journal 14, no. 1 (November 25, 2020): 266–77. http://dx.doi.org/10.2174/1874434602014010266.

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Background: Fusarium keratitis accounts for around 50% of mycotic keratitis cases. Major virulence factors produced by keratopathogenic fungi are proteases. Objective: The aim of the current study was to identify proteases contributing to corneal pathogenicity of Fusarium species. Methods: Culture filtrates from fourteen Fusarium solani species complex (FSSC) isolates and three F. delphinoides isolates were evaluated for protease activity and gelatine zymography. Mass spectroscopy was carried out using a partially purified enzyme and total extracellular extract. Protease gene expression in an in-vitro condition and an ex-vivo goat corneal infection model was measured using qRT-PCR. Specific activity was observed in a wide range and at a broad pH range; and isolates Cs1 (maximum) and Cc50 (minimum) were selected for the infection model. Results: Gene expression in in-vitro condition showed the highest fold change for proteases (C7YY94, C7Z7U2 and C7Z6W1) while in an ex-vivo infection highest fold change was seen for proteases (C7Z6W1, C7YQJ2 and C7Z7U2); in decreasing order, respectively. Expression of aminopeptidase (C7Z6W1) was 50-fold higher in the infected cornea in both isolates (Cs1 and Cc50); while expression of carboxypeptidase (C7YVF3) was 15-fold higher only in isolate Cs1. Corneal histology showed less penetration of Cc50 than Cs1 into the stroma. Mass spectrometry showed the presence of carboxypeptidase (C7YVF3) and tripeptidyl amino peptidase. Conclusion: It can be concluded that clinical isolates of FSSC produce varying amounts of proteases and differ in specific activity and gene expression in both conditions (in vitro and ex vivo). Carboxypeptidase and aminopeptidase contribute to the pathogenic potential of Fusarium solani species complex.

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Hernandez Arroyo, L., O. Santesteban Echarri, M. J. Güerre Lobera, J. C. Espin Jaime, and M. Á. Jimenez-Arriero. "Fears in anxious children." European Psychiatry 33, S1 (March 2016): S352. http://dx.doi.org/10.1016/j.eurpsy.2016.01.1249.

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IntroductionFear is a distressing emotion aroused by a risk or a damage, real or imaginary. Fears have a warning function against dangers. Nevertheless, fear can also become in one of the most limiting elements of a person's life.ObjectivesTo characterize the profile of fears presented in a sample of 19 children, aged between 8 and 13 years old, who had previously been diagnosed with one of the following: Generalized Anxiety Disorder, Separation Anxiety Disorder or Social Phobia.Material and methodsThe sample was taken from patients who participated in a cognitive-behavioral group therapy. The Spanish version of the Fear Survey Schedule for Children-Revised (FSSC-R) questionnaire was employed. The FSSC-R asks children to indicate on a 3-point scale (‘none’, ‘some’, ‘a lot’) how much they fear 80 specific stimuli or situations. Five basic categories of fears can be stablished: failure and criticism (17 items); the unknown (17 items); minor injury and small animals (13 items); danger and death (16 items); medical fears (5 items).ResultsThe sample includes 19 children: 12 boys (63.2%) and 7 girls (36.8%). The median age is 10.74 years. The sample shows 15 excessive fears on average (those scored as ‘a lot’). Girls show higher rates of excessive fears than boys: 19 versus 13. The most common fears were ‘being hit by a car or truck’, ‘bombing attacks. Being invaded’, ‘a burglar breaking into our house’ and ‘falling from high places’.ConclusionsMost common excessive fears belong to danger and death category. Females report more fears than males.Disclosure of interestThe authors have not supplied their declaration of competing interest.

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Lira, Vanessa Lopes, Ana Carla da Silva Santos, Patricia Vieira Tiago, Neiva Tinti de Oliveira, and Romero Marinho de Moura. "Hiperparasitismo de Fusarium spp. em Austropuccinia psidii em Jambo-do-Pará." Summa Phytopathologica 45, no. 2 (April 2019): 204–6. http://dx.doi.org/10.1590/0100-5405/187593.

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RESUMO Austropuccinia psidii é o agente etiológico de uma das principais doenças do jambeiro-do-pará: a ferrugem-amarela. Em uma ocorrência dessa doença foi constatada a ação de um fungo hiperparasito sobre uredossoro de A. psidii. A partir de folhas lesionadas, dois isolados de Fusarium foram caracterizados por análise morfológica e molecular. Os resultados revelaram que esses organismos pertencem a dois complexos de espécies: Fusarium fujikuroi (FFSC) e Fusarium solani (FSSC). A ocorrência desses fungos micoparasitas é uma forma de controle biológico natural para esta ferrugem e o seu estudo contribuirá para o manejo da ferrugem amarela.

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Di Riso, Daniela, Daphne Chessa, Elisa Delvecchio, Andrea Bobbio, Silvia Salcuni, Adriana Lis, and Thomas H. Ollendick. "Structure of the Italian Fear Survey Schedule for Children (FSSC-IT)." Journal of Psychology 147, no. 6 (November 2013): 577–97. http://dx.doi.org/10.1080/00223980.2012.727890.

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Kotelevych, V. A. "Veterinary and sanitary assessment of quality and safety of meat and meat products in the marshes of LLC “Riton” Vinnytsa." Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 20, no. 88 (September 8, 2018): 24–28. http://dx.doi.org/10.32718/nvlvet8804.

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The purpose of our research was to study the environmental aspects of the quality and safety of meat and meat products in the conditions of Ltd “Ryton” Vinnytsya and the measures applied in this subsidiary for the observance of proper hygienic and production practices (GHP/GMP) in their production as a guarantor of environmental security. The results of the veterinary and sanitary estimation of meat and meat products are reported according to the data of the reporting documentation of Ltd Ryton Vinnytsia (2015–2017), expert opinions of the Vinnytsia Regional State Veterinary Laboratory and Khmelnytsky Interdistrict State Laboratory of Veterinary Medicine (2018) on indicators security. The main cause of detoxification of byproducts was invasive and non-contagious diseases, in particular: liver – cirrhosis, fasciolysis and abscesses; kidneys – jade heart – traumatic pericarditis; udder – mastitis, lungs – pneumonia, pleurisy, aspiration with food and blood. According to safety indicators, there is a lack of pesticides (the mass fraction of HCCh gamma-isomer, DDT and its metabolites, DDVF, chlorophos, metaphys, carbophos, basidins), toxic elements (lead, cadmium, arsenic, mercury, copper, zinc), mycotoxins, antibiotics; Radiological and microbiological parameters in meat and meat samples were also within regulatory requirements and safe for consumption. The introduction of proper hygienic and production practices allowed Vinnitsa LLC to complete the certification process under the international program FSSC 22000 “Food Safety Management” and obtain the Certificates The HACCP Management System of CCM MS Standart HACCP and ISO 22000: 2007 Food Safety Management Systems, Requirements for any Organisation in the Food Chain. The performed researches prove the theoretical and practical justification of the necessity of monitoring research on the quality and safety of livestock products, the observance of proper hygienic and production practices (GHP/GMP) taking into account the international program FSSC 22000 “Food Safety Management”, – the need to strengthen preventive measures by doctors of veterinary medicine invasive and non-contagious diseases of cattle.

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Douriet-Angulo, A., C. A. López-Orona, G. A. López-Urquídez, T. A. Vega-Gutiérrez, M. A. Tirado-Ramírez, M. D. Estrada-Acosta, F. Ayala-Tafoya, and M. G. Yáñez-Juárez. "Maize Stalk Rot Caused by Fusarium falciforme (FSSC 3 + 4) in Mexico." Plant Disease 103, no. 11 (November 2019): 2951. http://dx.doi.org/10.1094/pdis-05-19-1055-pdn.

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Jones, E. B. Gareth, Bandarupalli Devadatha, Mohamed A. Abdel-Wahab, Monika C. Dayarathne, Sheng-Nan Zhang, Kevin D. Hyde, Jian-Kui (Jack) Liu, et al. "Phylogeny of new marine Dothideomycetes and Sordariomycetes from mangroves and deep-sea sediments." Botanica Marina 63, no. 2 (March 26, 2020): 155–81. http://dx.doi.org/10.1515/bot-2019-0014.

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AbstractThis paper documents six new saprobic marine fungi and one new genus based on morphology and multi-gene phylogenies. Three Dothideomycetes, and members of the Pleosporales, are introduced: Pseudomassariosphaeria triseptata sp. nov. was recognized as a mangrove species in Amniculicolaceae, and Salsuginea phoenicis sp. nov. was discovered as a second member of Salsugineaceae. A new genus Raghukumaria with Raghukumaria keshaphalae sp. nov., recovered from mangroves, is phylogenetically sister to Halomassarina and nests in the Trematosphaeriaceae. Three new species are referred to the Sordariomycetes: Coniochaeta marina (Coniochaetales, Coniochaetaceae) on driftwood; Fusicolla bharatavarshae (Hypocreales, Nectriaceae) is introduced with asexual and sexual morphs, on decayed mangrove wood of Avicennia marina; and Fusarium sedimenticola (Hypocreales, Nectriaceae) is new to the Fusarium solani species complex (FSSC) from deep-sea sediment.

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Zamula, V. S., and Yu A. Kuzlyakina. "Features of the FSSC 22000 certification scheme when implemented in the meat industry." Meat Industry Journal, no. 1 (2021): 33–37. http://dx.doi.org/10.37861/2618-8252-2021-01-33-36.

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Bomba, M. Ya, and N. Ya Susol. "Main requirements for food safety management systems under international standards: BRС, IFS, FSSC 22000, ISO 22000, Global GAP, SQF." Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 22, no. 93 (May 13, 2020): 18–25. http://dx.doi.org/10.32718/nvlvet-f9304.

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The research analyzed the international standards basic requirements structure: BRС, IFS, FSSC 22000, ISO 22000, Global GAP, SQF for food safety management systems that realize principles of the HACCP concept. A comparative analysis of the international standards basic requirements for food safety management systems has identified that the requirements structure is identified as an interconnected set of rules, system-structured processes for the purposeful prevention of hazard risks at certain stages or in the food business. The main differences between the standards are in the modification of approaches to the implementation of the HACCP principles, the interpretation of the basic concepts and definitions, the detailed requirements, the application of their own programs of the processes and procedures identification that allow to ensure that the results correspond to the set task. Other differences in standards are at the level of system-elemental, structural, and functional components. Standards have the same goals, so their requirements are similar and have a certain level of identity, much of the difference is at the audit level, which uses different levels, system points and categories. The requirements of all standards are structured and differentiated into mandatory and recommended blocks, which enables companies to gradually implement changes. IFS, FSSC, ISO standards have high level structure (HLS), which is common basis for ISO standards that greatly simplifies the integration of several systems simultaneously in monitoring, action adjustments, audit processes. The GlobalGAP standard requirement system, unlike other considered ones, has a narrow target, which has provided a detailed description, for tracking, a set of indicators for quality and safety including genetically modified organisms and allergens, however, it is compatible with others. Taking into account the globalization of markets conditions in analyzing the international standards requirements, their correlation with global safety-related criteria such as: implementation of the HACCP principles, recognition of the standard GFSI, providing traceability principles and mechanisms and prerequisites for programs (PRP), validity of the certificate, coherence of processes in the creation of integrated systems with standards ISO 9001, ISO 14001, OHSAS 18000.

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Bouvard, Martine, and Jean-Luc Roulin. "Confirmation des dimensions de l’échelle des peurs pour enfants révisée (FSSC-R) d’Ollendick (1983)." Journal de Thérapie Comportementale et Cognitive 22, no. 1 (March 2012): 12–15. http://dx.doi.org/10.1016/j.jtcc.2012.01.005.

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Debourgogne, Anne, Cécile Gueidan, Christophe Hennequin, Nelly Contet-Audonneau, Sybren de Hoog, and Marie Machouart. "Development of a new MLST scheme for differentiation of Fusarium solani Species Complex (FSSC) isolates." Journal of Microbiological Methods 82, no. 3 (September 2010): 319–23. http://dx.doi.org/10.1016/j.mimet.2010.07.008.

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Vega-Gutiérrez, T. A., C. A. López-Orona, G. A. López-Urquídez, S. Velarde-Félix, L. A. Amarillas-Bueno, A. R. Martínez-Campos, and R. Allende-Molar. "Foot Rot and Wilt in Tomato Caused by Fusarium falciforme (FSSC 3 + 4) in Mexico." Plant Disease 103, no. 1 (January 2019): 157. http://dx.doi.org/10.1094/pdis-06-18-1001-pdn.

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Moraes, Antonio Bento Alves de, Gláucia Maria Bovi Ambrosano, Rosana de Fátima Possobon, and Áderson Luiz Costa Junior. "Fear assessment in brazilian children: the relevance of dental fear." Psicologia: Teoria e Pesquisa 20, no. 3 (December 2004): 289–94. http://dx.doi.org/10.1590/s0102-37722004000300011.

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Fear frequently interferes with dental treatment procedures, producing delays and poor technical quality results. Patients exhibit avoidance and escape behaviors that may be related to aversive childhood experiences in dental treatment situations. The aim of this study was to identify the most frequent children's fears, including dental ones, using an adaptation of the FSSC-R. This instrument was used to assess 549 children divided in three groups: (G1) private school children, (G2) public school children and (G3) public school children who were surveyed during dental treatment. Results indicated higher dental fear scores for females when compared with male children. Considering all groups studied, "injection" was the 5th higher fear for the group G3, 8th for the group G1 and 14th for the group G2. Family relationship items such as "parents fighting", "parents yelling at you", "hearing my parents argue", were considered frequent fears, suggesting that family conflicts may contribute to children's emotional problems.

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Gullone, Eleonora, Neville J. King, Bruce Tonge, David Heyne, and Thomas H. Ollendick. "The Fear Survey Schedule for Children - II (FSSC-II): Validity Data as a Treatment Outcome Measure." Australian Psychologist 35, no. 3 (November 2000): 238–43. http://dx.doi.org/10.1080/00050060008257485.

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Last, Cynthia C., Greta Francis, and Cyd C. Strauss. "Assessing Fears in Anxiety-Disordered Children With the Revised Fear Survey Schedule for Children (FSSC-R)." Journal of Clinical Child Psychology 18, no. 2 (June 1989): 137–41. http://dx.doi.org/10.1207/s15374424jccp1802_4.

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Shen, Dong-Xia, Zong-Wen Song, Yi-Ming Lu, and Ben Fan. "First Report of Fusarium falciforme (FSSC 3+4) Causing Root Rot in Weigela florida in China." Plant Disease 104, no. 3 (March 2020): 981. http://dx.doi.org/10.1094/pdis-03-19-0521-pdn.

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Vega-Gutiérrez, T. A., M. A. Tirado-Ramírez, G. A. López-Urquídez, A. Angulo-Castro, J. A. Martínez-Gallardo, and C. A. López-Orona. "Fusarium falciforme (FSSC 3 + 4) Causing Root and Stem Rot in Papaya (Carica papaya) in Mexico." Plant Disease 103, no. 10 (October 2019): 2681. http://dx.doi.org/10.1094/pdis-05-19-0917-pdn.

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Maryani, N., M. Sandoval-Denis, L. Lombard, P. W. Crous, and G. H. J. Kema. "New endemic Fusarium species hitch-hiking with pathogenic Fusarium strains causing Panama disease in small-holder banana plots in Indonesia." Persoonia - Molecular Phylogeny and Evolution of Fungi 43, no. 1 (December 31, 2019): 48–69. http://dx.doi.org/10.3767/persoonia.2019.43.02.

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Fusarium species are well known for their abundance, diversity and cosmopolitan life style. Many members of the genus Fusarium are associated with plant hosts, either as plant pathogens, secondary invaders, saprotrophs, and/or endophytes. We previously studied the diversity of Fusarium species in the Fusarium oxysporum species complex (FOSC) associated with Fusarium wilt of banana in Indonesia. In that study, several Fusarium species not belonging to the FOSC were found to be associated with Fusarium wilt of banana. These Fusarium isolates belonged to three Fusarium species complexes, which included the Fusarium fujikuroi species complex (FFSC), Fusarium incarnatum-equiseti species complex (FIESC) and the Fusarium sambucinum species complex (FSSC). Using a multi-gene phylogeny that included partial fragments of the beta-tubulin (tub), calmodulin (cmdA), translation elongation factor 1-alpha (tef1), the internal transcribed spacer region of the rDNA (ITS), the large subunit of the rDNA (LSU), plus the RNA polymerase II large subunit (rpb1) and second largest subunit (rpb2) genes, we were able to identify and characterise several of these as new Fusarium species in the respective species complexes identified in this study.

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Journal articles on the topic 'FSSC'

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