Relevant bibliographies by topics / Full-length clone

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Full-length clone'

Author: Grafiati
Published: 7 June 2025
Last updated: 2 August 2025

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Journal articles on the topic "Full-length clone"

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Wolf, D., N. Harris, N. Goldfinger, and V. Rotter. "Isolation of a full-length mouse cDNA clone coding for an immunologically distinct p53 molecule." Molecular and Cellular Biology 5, no. 1 (1985): 127–32. http://dx.doi.org/10.1128/mcb.5.1.127-132.1985.

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Transfection of a cloned p53 gene into a p53 nonproducer Abelson murine leukemia virus-transformed cell line, L12, reconstituted p53 expression. The protein expressed in these cells was indistinguishable from that naturally expressed in p53 producer tumor cells. Conversely, p53 protein expressed in L12-derived clones that were established by transfection with a full-length p53 cDNA clone (pM8) exhibited a discrete immunological form. Immunoprecipitation of p53 with a panel of monoclonal anti-p53 antibodies showed that L12-derived clones that were transfected with the genomic p53 clone containe
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Wolf, D., N. Harris, N. Goldfinger, and V. Rotter. "Isolation of a full-length mouse cDNA clone coding for an immunologically distinct p53 molecule." Molecular and Cellular Biology 5, no. 1 (1985): 127–32. http://dx.doi.org/10.1128/mcb.5.1.127.

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Transfection of a cloned p53 gene into a p53 nonproducer Abelson murine leukemia virus-transformed cell line, L12, reconstituted p53 expression. The protein expressed in these cells was indistinguishable from that naturally expressed in p53 producer tumor cells. Conversely, p53 protein expressed in L12-derived clones that were established by transfection with a full-length p53 cDNA clone (pM8) exhibited a discrete immunological form. Immunoprecipitation of p53 with a panel of monoclonal anti-p53 antibodies showed that L12-derived clones that were transfected with the genomic p53 clone containe
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Dunn, Stephen J., Richard D. Oberst, Jeffrey L. Stott, and Bennie I. Osburn. "Molecular cloning of serogroup- and serotype-specific genome segments from bluetongue virus serotype 11." American Journal of Veterinary Research 50, no. 10 (1989): 1684–89. https://doi.org/10.2460/ajvr.1989.50.10.1684.

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SUMMARY Genome segments 2, 6, 8, and 9 of bluetongue virus (btv) serotype 11, coding for P2, NS1, NS2, and P6, respectively, were cloned into pUC 8. Sizes of segment-2 and segment-6 clones indicated partial copies (55% and 80% of full length, respectively), whereas segment 8 and 9 clones represented full-length copies. Northern blot hybridizations of the clones to the 5 United States btv prototypic serotypes (2, 10, 11, 13, and 17) revealed segment-2 clone to be serotype-specific to btv-11, whereas segment 6, 8, and 9 clones were able to detect all serotypes to varying degrees. All clones fail
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Nielsen, H. S., G. Liu, J. Nielsen, et al. "Generation of an Infectious Clone of VR-2332, a Highly Virulent North American-Type Isolate of Porcine Reproductive and Respiratory Syndrome Virus." Journal of Virology 77, no. 6 (2003): 3702–11. http://dx.doi.org/10.1128/jvi.77.6.3702-3711.2003.

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ABSTRACT A full-length cDNA clone of the prototypical North American porcine reproductive and respiratory syndrome virus (PRRSV) isolate VR-2332 was assembled in the plasmid vector pOK12. To rescue infectious virus, capped RNA was transcribed in vitro from the pOK12 clone and transfected into BHK-21C cells. The supernatant from transfected monolayers were serially passaged on Marc-145 cells and porcine pulmonary alveolar macrophages. Infectious PRRSV was recovered on Marc-145 cells as well as porcine pulmonary macrophages; thus, the cloned virus exhibited the same cell tropism as the parental
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Meulenberg, J. J. M., J. N. A. Bos-de Ruijter, R. van de Graaf, G. Wensvoort, and R. J. M. Moormann. "Infectious Transcripts from Cloned Genome-Length cDNA of Porcine Reproductive and Respiratory Syndrome Virus." Journal of Virology 72, no. 1 (1998): 380–87. http://dx.doi.org/10.1128/jvi.72.1.380-387.1998.

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ABSTRACT The 5′-terminal end of the genomic RNA of the Lelystad virus isolate (LV) of porcine reproductive and respiratory syndrome virus was determined. To construct full-length cDNA clones, the 5′-terminal sequence was ligated to cDNA clones covering the complete genome of LV. When RNA that was transcribed in vitro from these full-length cDNA clones was transfected into BHK-21 cells, infectious LV was produced and secreted. The virus was rescued by passage to porcine alveolar lung macrophages or CL2621 cells. When infectious transcripts were transfected to porcine alveolar lung macrophages o
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Liu, Guangqing, Yuying Zhang, Zheng Ni, et al. "Recovery of Infectious Rabbit Hemorrhagic Disease Virus from Rabbits after Direct Inoculation with In Vitro-Transcribed RNA." Journal of Virology 80, no. 13 (2006): 6597–602. http://dx.doi.org/10.1128/jvi.02078-05.

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ABSTRACT We report the first full-length infectious clone of strain JX/CHA/97 of rabbit hemorrhagic disease virus (RHDV). The transcripts from the full-length cDNA clones were infectious when they were directly injected into rabbits. The sequence of the virus recovered from the rabbits was identical to that of the injected RNA transcripts. The cDNA clone was engineered to contain one silent nucleotide change to create an EcoRV site (A to T at nucleotide 2908). The genetic marker was retained in the recovered progeny virus. The transfection of RNA transcripts into RK-13 cells resulted in the sy
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Wolf, D., Z. Laver-Rudich, and V. Rotter. "In vitro expression of human p53 cDNA clones and characterization of the cloned human p53 gene." Molecular and Cellular Biology 5, no. 8 (1985): 1887–93. http://dx.doi.org/10.1128/mcb.5.8.1887-1893.1985.

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The human p53 gene was cloned and characterized by using a battery of p53 DNA clones. A series of human cDNA clones of various sizes and relative localizations to the mRNA molecule were isolated by using the human p53-H14 (2.35-kilobase) cDNA probe which we previously cloned. One such isolate, clone p53-H7 (2.65 kilobases), spans the entire human mature p53 mRNA molecule. Construction of the human cDNA clones in the pSP65 RNA transcription vector facilitated the generation of p53 transcripts by the SP6 bacteriophage RNA polymerase. The p53-specific RNA transcripts obtained without further proc
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Wolf, D., Z. Laver-Rudich, and V. Rotter. "In vitro expression of human p53 cDNA clones and characterization of the cloned human p53 gene." Molecular and Cellular Biology 5, no. 8 (1985): 1887–93. http://dx.doi.org/10.1128/mcb.5.8.1887.

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The human p53 gene was cloned and characterized by using a battery of p53 DNA clones. A series of human cDNA clones of various sizes and relative localizations to the mRNA molecule were isolated by using the human p53-H14 (2.35-kilobase) cDNA probe which we previously cloned. One such isolate, clone p53-H7 (2.65 kilobases), spans the entire human mature p53 mRNA molecule. Construction of the human cDNA clones in the pSP65 RNA transcription vector facilitated the generation of p53 transcripts by the SP6 bacteriophage RNA polymerase. The p53-specific RNA transcripts obtained without further proc
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Lanford, Robert E., Helen Lee, Deborah Chavez, Bernadette Guerra, and Kathleen M. Brasky. "Infectious cDNA clone of the hepatitis C virus genotype 1 prototype sequence." Journal of General Virology 82, no. 6 (2001): 1291–97. http://dx.doi.org/10.1099/0022-1317-82-6-1291.

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A full-length cDNA clone of the hepatitis C virus (HCV) genotype 1 prototype (subtype 1a) sequence was constructed. Synthetic RNA produced from the initial cDNA clone was not infectious following intrahepatic inoculation of a chimpanzee. A consensus clone was prepared by comparison with multiple full-length HCV sequences of genotypes 1, 2 and 3. A total of 11 non-consensus amino acid residues were altered by mutagenesis. Synthetic RNA from the repaired clone initiated a typical, acute-resolving HCV infection following intrahepatic inoculation of a chimpanzee. In addition, at least one of three
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Harlow, E., N. M. Williamson, R. Ralston, D. M. Helfman, and T. E. Adams. "Molecular cloning and in vitro expression of a cDNA clone for human cellular tumor antigen p53." Molecular and Cellular Biology 5, no. 7 (1985): 1601–10. http://dx.doi.org/10.1128/mcb.5.7.1601-1610.1985.

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Three clones for the human tumor antigen p53 were isolated from a cDNA library prepared from A431 cells. One of these clones, pR4-2, contains the entire coding region for human p53. This clone directs the synthesis of a polypeptide with the correct molecular weight and immunological epitopes of an authentic p53 molecule in an in vitro transcription-translation reaction. Although the pR4-2 clone contains the coding region for p53, it is not a full-length copy of the human p53 mRNA. Northern analysis showed that the p53 mRNA is approximately 2,500 nucleotides long, whereas the pR4-2 insert is on
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Dissertations / Theses on the topic "Full-length clone"

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Skuce, Robin Alfred. "Construction of a full-length infectious cDNA clone of a bovine enterovirus." Thesis, Queen's University Belfast, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.356921.

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Yuan, Fangfeng. "Construction and characterization of a full-length complementary DNA infectious clone of emerging porcine Senecavirus A." Thesis, Kansas State University, 2017. http://hdl.handle.net/2097/35511.

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Master of Science<br>Department of Diagnostic Medicine/Pathobiology<br>Ying Fang<br>Seneca Valley Virus (SVV) causes vesicular disease in pigs. Vesicular lesions on the snout and coronary band of hoof mostly resemble lesions caused by Foot-and-Mouth Disease Virus (FMDV), which may lead to the foreign animal disease investigation. In 2015, Brazil experienced major outbreaks of SVV; then in July, sporadic cases of SVV were reported in United States and became a concern in swine industry. A reverse-genetic system serves as a major tool to study pathogenesis of the virus. In our study, a full-leng
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Law, Ka-Yu. "Isolation and Characterization of Plastidic Glucose-6-Phosphate Dehydrogenase (G6PDH) from Castor (Ricinus communis L.)." Thesis, Kingston, Ont. : [s.n.], 2007. http://hdl.handle.net/1974/722.

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Hasan, Hana'a. "Generation of an infectious Beet mosaic virus (BtMV) full-length clone based on the complete nucleotide sequence of a German isolate." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=974372692.

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Novelli, Patricia. "Construction and characterisation of full length HIV 1 infectious molecular clones." Thesis, Queen Mary, University of London, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.271472.

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Bunawan, Hamidun. "Infectious full-length clones of Cassava brown streak virus : towards functional genomic studies." Thesis, University of Bristol, 2016. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.702169.

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Cassava is a staple crop for over 500 million people worldwide. Production of cassava is affected by several constraints, including viral diseases such as cassava brown streak disease (CBSD), caused by the ipomoviruses Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). CBSD is present throughout cassava growing regions in Africa and symptoms include streaking, chlorosis, dieback and tuber necrosis, leading to crop losses of up to 70%. An increased genetic and molecular understanding of the viruses that cause CBSD is essential for management of this disease. In th
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Nanyiti, Sarah. "Construction and characterization of Ugandan cassava brown streak virus (UCBSV) full-length infectious clones." Thesis, University of Bristol, 2016. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.702885.

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Zhang, Lei [Verfasser], and Wilhelm [Akademischer Betreuer] Jelkmann. "Construction of infectious full-length cDNA clones of apple viruses and plant viral vector development / Lei Zhang ; Betreuer: Wilhelm Jelkmann." Heidelberg : Universitätsbibliothek Heidelberg, 2017. http://d-nb.info/1178010139/34.

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Bald-Blume, Niklas [Verfasser]. "Luminex xTAG detection of Cucumber mosaic virus (CMV) and different tospoviruses as well as further characterization of CMV with infectious full-length clones and pseudorecombinants / Niklas Bald-Blume." Hannover : Technische Informationsbibliothek (TIB), 2016. http://d-nb.info/1122664087/34.

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Hasan, Hana'a [Verfasser]. "Generation of an infectious Beet mosaic virus (BtMV) full-length clone based on the complete nucleotide sequence of a German isolate / von Hana'a Hasan." 2004. http://d-nb.info/974372692/34.

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Books on the topic "Full-length clone"

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D'Amario, Dan. Real close to Broadway!: A full-length comedy. Eldridge Pub., 2007.

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Honbu, Seihin Hyōka Gijutsu Kiban Kikō Baiotekunorojī. NBRC catalogue of biological resources: Microorganisms, genomic DNA clones, and human full-length cDNA clones. Department of Biotechnology, National Institute of Technology and Evaluation, 2005.

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Gellatly, Duncan Laurence. Infectivity of full-length clones of the viroid-like sattelite RNA of lucerne transient streak virus. National Library of Canada, 1993.

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Klavins, Maris Herbert. Biochemical criteria for the differentiation of clinical phenotypes in O-variant GM r gangliosidosis. Isolation and characterization of a cDNA clone coding for the full length polypeptide chain of human hexosaminidase. 1987.

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Fishburn, Katherine. Reading Buchi Emecheta. Greenwood Publishing Group, Inc., 1995. http://dx.doi.org/10.5040/9798216005292.

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In this first full-length study of Emecheta's fiction, Fishburn highlights the difficulties inherent in reading across cultures. She challenges the notion that all we need to understand African texts is a willingness to be open to them, arguing that too many of the cultural and critical preconceptions we bring to these texts interfere with our ability to understand them. Directly responding to Western feminist criticism written about Emecheta, this study argues that Emecheta herself is not a feminist in the Western sense and that her novels should not be construed as reflecting this political
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Thomas, Edmund. Monumentality and the Roman Empire. Oxford University Press, 2007. http://dx.doi.org/10.1093/oso/9780199288632.001.0001.

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The quality of "monumentality" is attributed to the buildings of few historical epochs or cultures more frequently or consistently than to those of the Roman Empire. It is this quality that has helped to make them enduring models for builders of later periods. This extensively illustrated book, the first full-length study of the concept of monumentality in Classical Antiquity, asks what it is that the notion encompasses and how significant it was for the Romans themselves in molding their individual or collective aspirations and identities. Although no single word existed in antiquity for the
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Ryan, Kiernan. Shakespearean Tragedy. Bloomsbury Publishing Plc, 2021. http://dx.doi.org/10.5040/9781472587022.

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This ground-breaking book reveals the prophetic, revolutionary vision that drives Shakespeare’s tragedies, tracing its unbroken development from its beginnings in the Henry VI plays and Shakespeare’s first tragedy, Titus Andronicus, right through to his last, Coriolanus. The four full-length studies at the heart of the book focus in depth on Shakespeare’s four greatest tragedies: Hamlet, Othello, King Lear and Macbeth. Shakespearean Tragedy engages with each of these titanic masterpieces as a singular, complete work of dramatic art with its own distinctive concerns and critical challenges, but
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Sternlicht, Sanford. Chaim Potok. Greenwood, 2000. http://dx.doi.org/10.5040/9798400624353.

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Since the publication of his first novel,The Chosen, Chaim Potok has been regarded as one of the most important Jewish-American writers of our time. In that 1967 landmark work, in its sequelThe Promise(1969), and in the other works that followed, Potok has explored the conflict between Jewish values and the secular American culture against which these enlightening stories are set. This full-length critical study introduces students to the powerful fiction of Potok. By examining in depth not only the spiritual elements but also the literary components that make works such asMy Name Is Asher Lev
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Songer, Marcia. Garrison Keillor. Greenwood, 2000. http://dx.doi.org/10.5040/9798400655647.

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Combining a career in live radio performances and equally lively short story writing for theNew Yorker,Garrison Keillor has continually charmed fans and readers with his homespun wit and warmth. While acknowledging his career highlights, this full-length critical study supports Keillor's own view of himself as writer rather than performer, by examining his literary accomplishments and giving serious analysis of his fictional works. In order to understand his tremendous popular appeal, Songer situates Keillor within the rich literary heritage associated with American humorists such as Mark Twai
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Williams, James. Edward Lear. Liverpool University Press, 2018. http://dx.doi.org/10.3828/liverpool/9780746312216.001.0001.

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Edward Lear wrote a well-known autobiographical poem that begins “How pleasant to know Mr Lear!” But how well do we really know him? On the one hand he is, in John Ashbery’s words, “one of the most popular poets who ever lived”; on the other hand he has often been overlooked or marginalized by scholars and in literary histories. This book, the first full length critical study of the poet since the 1980s, sets out to re-introduce Lear and to accord him his proper place: as a major Victorian figure of continuing appeal and relevance, and especially as a poet of beauty, comedy, and profound ingen
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Book chapters on the topic "Full-length clone"

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Fang, Ying, Kay S. Faaberg, Raymond R. R. Rowland, et al. "Construction of a Full-Length cDNA Infectious Clone of a European-Like Type 1 PRRSV Isolated in the U.S." In Advances in Experimental Medicine and Biology. Springer US, 2006. http://dx.doi.org/10.1007/978-0-387-33012-9_110.

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Salter, A. Hugh, and John C. Gray. "Characterisation of a Full-Length cDNA Clone Encoding the Pea Rieske Fe-S Protein : Import and Processing by Isolated Chloroplasts." In Current Research in Photosynthesis. Springer Netherlands, 1990. http://dx.doi.org/10.1007/978-94-009-0511-5_496.

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Johansen, I. Elisabeth, and Ole Søgaard Lund. "Insertion of Introns: A Strategy to Facilitate Assembly of Infectious Full Length Clones." In Plant Virology Protocols. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-102-4_36.

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Li, Yinzi, and Aiming Wang. "Biolistic Inoculation of Fruit Trees with Full-Length Infectious cDNA Clones of RNA Viruses." In Methods in Molecular Biology. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1835-6_20.

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Sola, Isabel, Sara Alonso, Carlos Sanchez, J. Manuel Sanchez-Morgado, and Luis Enjuanes. "Expression of Transcriptional Units Using Transmissible Gastroenteritis Coronavirus Derived Minigenomes and Full-length cDNA Clones." In Advances in Experimental Medicine and Biology. Springer US, 2001. http://dx.doi.org/10.1007/978-1-4615-1325-4_65.

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Lutz, Alexander, and Axel Lachmeyer. "SciPPPer: Automatic Lock-Passage for Inland Vessels – Practical Results Focusing on Control Performance." In Lecture Notes in Civil Engineering. Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-19-6138-0_85.

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AbstractNavigating through locks is one of the most challenging tasks that skippers have to perform in inland navigation. Typical dimensions of a ship (width = 11.45 m) and a lock (width = 12 m) result in an error margin of less than 30 cm to the left and to the right of the ship when navigating within a lock chamber. Typical inland vessels on European waters have a length of 82 to 186 m. The wheel house on cargo vessels is located close to the stern of the vessel. This leads to low visibility of the bow in the lock chamber. In order to cope with this issue, a deck hand monitors the bow and an
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Clarke, David. "3. Explorations and Analyses (I)." In Rāgs Around the Clock. Open Book Publishers, 2024. http://dx.doi.org/10.11647/obp.0313.03.

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This part of the book contains two discussions: the first (section 3.2) asks ‘How do you sing an ālāp?’, and the second (section 3.3) asks ‘How do you sing a choṭā khayāl?’ Section 3.2 is an article-length discussion that investigates the procedures of ālāp, the unmetred opening stage of an Indian classical performance, and one of its fundamental principles of extemporisation. I approach the question, how do you sing an ālāp? through three explorations, each involving close musical analysis of extracts from Rāg samay cakra—thus also providing commentary on the album. In the first and longest,
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Shisheva, Assia, and Michael P. Czech. "Rab-GDI2." In Guidebook to the Sinall GTPases. Oxford University PressOxford, 1995. http://dx.doi.org/10.1093/oso/9780198599456.003.0117.

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Abstract A full-length mouse GDI2 cDNA was isolated by screening a mouse skeletal muscle cDNA library with the full-length rat brain GDI1 cDNA (Shisheva et al. 1994). A cDNA clone found to be the mouse homolog of the previously reported bovine brain smg p25A-GDI (Matsui et al. 1990) and rat brain GDI1 (Shisheva etal. 1994) was also isolated from the mouse skeletal muscle library and is denoted mGDI1 (Shisheva et al. 1994). The DNA sequence of mGDI2 is presented in Fig. 1. The GenBank accession number is UO7951.
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Kahn, Richard A. "ArL3." In Guidebook to the Sinall GTPases. Oxford University PressOxford, 1995. http://dx.doi.org/10.1093/oso/9780198599456.003.0149.

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Abstract The partial cDNA sequence was first reported as one of many expressed sequence tags (EST) by Adams, et al (Adams et al. 1992; accession number M85466) as clone HFBCI57. Both strands of this clone were fully sequenced and 5’ RACE was performed to obtain the full-length open reading frame (Cavenagh etal. 1994). A rat homologue of ARL3 was obtained independently by workers in the laboratory of Dr Hans Joost (Cavenagh et al. 1994).
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Hall, Alan. "Rho-GAP." In Guidebook to the Sinall GTPases. Oxford University PressOxford, 1995. http://dx.doi.org/10.1093/oso/9780198599456.003.0074.

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Abstract A full-length Rho-GAP clone has been isolated from a human fibrosarcoma cDNA library (Lancaster eta/. 1994). A single mRNA of approximately 3600 nucleotides encodes a protein of 50 kDa. The nucleotide sequence of human p50 Rho-GAP and its deduced amino acid sequence are shown in Fig. 1. The GenBank accession number is Z23024.
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Conference papers on the topic "Full-length clone"

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Pawson, R. L. "Close Interval Potential Surveys Planning, Execution, Results." In CORROSION 1997. NACE International, 1997. https://doi.org/10.5006/c1997-97575.

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Abstract Cathodic protection surveys are an integral part of maintaining safe pipeline operation. Such surveys are mandated by law in many countries of the world. One of the standard means of testing cathodic protection system operation and pipeline protection levels, is the annual or bi-annual test point survey. These tests normally provide potential data on less than 0.5% of the pipeline. Computerized dataloggers for cathodic protection system monitoring enabled the development of the close interval survey (C.I.S.) into a viable means of assessing the effectiveness of cathodic protection sys
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Bosch, C., A. Liessem, and A. Kulgemeyer. "Full-Size Four-Point-Bend Testing - a New Approach to Evaluate the SSC Resistance of Large-Diameter Pipes for Sour Service." In CORROSION 2004. NACE International, 2004. https://doi.org/10.5006/c2004-04130.

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Abstract For investigation of the resistance of submerged arc welded (SAW) large-diameter pipes for application in sour service, a new full-size four-point-bend test method has been developed for performance of SSC tests on specimens of full pipe wall thickness. 45 specimen holders, designed for stressing specimens containing the original pipe curvature with a load up to 4 tons were machined. Specimens of 260 mm length, 25 mm width and up to 33 mm thickness were tested in solution A to NACE TM0177-96 at 0.1 MPa H2S and exposure times of 96 and 720 hours, respectively. The specimens had been ma
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Parker, Keith, Christophe Baete, and Trey Johnston. "Validating Digital Twin Model of a Complex Pipeline System." In CONFERENCE 2023. AMPP, 2023. https://doi.org/10.5006/c2023-18958.

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Abstract Operating a pipeline requires regular field surveys for controlling external corrosion threats. Surveys are typically performed at rectifiers and test stations, and occasionally a close interval survey between test stations is performed. Pipeline surveys provide only an indirect measure of the corrosion threat and are either labor intensive or do not provide sufficient granularity and accuracy to pinpoint corrosion features in a timely manner. A computational model of the primary and third party (crossing) pipelines and associated cathodic protection systems is built and further calib
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Bosma, P. J., E. A. van den Berg, and T. Kooistra. "ISOLATION OF THE GENE CODING FOR HUMAN PLASMINOGEN ACTIVATOR INHIBITOR TYPE 1 (PAI-1)." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644440.

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A human placenta genomic DNA cosmid library was screened for the presence of the PAI-1 gene using a cDNA probe coding for PAI-1. Two overlapping recombinant cosmids were obtained that contain human DNA spanning 55 kb. The cosmids were mapped using 3' and 5' end probes isolated from an almost full-length cDNA clone of 2.5 kb. The two cosmids were found to contain the entire structural PAI-1 gene (approximately 15 kb) and also included 25 kb 5' flanking sequences. The transcription initiation site was identified by SI nuclease protection experiments and the promotor region was sequenced. Further
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KIKUCHI, SHOSHI. "MASSIVE COLLECTION OF FULL-LENGTH COMPLEMENTARY DNA CLONES AND MICROARRAY ANALYSES: KEYS TO RICE TRANSCRIPTOME ANALYSIS." In Quantum Bio-Informatics II - From Quantum Information to Bio-Informatics. WORLD SCIENTIFIC, 2009. http://dx.doi.org/10.1142/9789814273756_0023.

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Dyment, Nathaniel A., Namdar Kazemi, Lindsey E. Aschbacher-Smith, et al. "The Relationships Among Spatiotemporal Gene Expression, Histology, and Biomechanics Following Full-Length Injury in the Murine Patellar Tendon." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53622.

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Tendon and ligament injuries present a considerable socioeconomic impact as close to 50% of the 32 million musculoskeletal injuries in the US per year include these structures [1]. The inadequate healing in these tissues requires novel treatment modalities. Improving tendon tissue engineering dictates that we better understand the process of natural adult tendon healing. Type-I (Col1) and Type-II (Col2) collagens are important structural proteins in tendon as Col1 is the main collagen type found in the tendon midsubstance while Col2 is expressed at the insertion into bone during development, g
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Menon, S. "CO Emission and Combustion Dynamics Near Lean-Blowout in Gas Turbine Engines." In ASME Turbo Expo 2004: Power for Land, Sea, and Air. ASMEDC, 2004. http://dx.doi.org/10.1115/gt2004-53290.

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Lean-Blowout (LBO) is a phenomenon that occurs in both land-based premixed and propulsion liquid-fuelled gas turbine engines when the effective equivalence ratio is reduced close to the lean flammability limit. Small perturbations in the flame or flow can result in local quenching that can subsequently lead to total extinction (LBO). Prediction of pollutant emission (e.g., CO) and combustion dynamics near LBO is very complicated since physics at many interacting scales have to be resolved. Here, LES studies of both premixed and liquid-fuelled gas turbine engines are reported using a subgrid li
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Gifer, P. K., V. I. Vernovskaya, and O. V. Batishchev. "STUDY OF MEMBRANE INTERACTIONS OF THE NM DOMAIN OF THE M1 PROTEIN OF INFLUENZA A VIRUS." In X Международная конференция молодых ученых: биоинформатиков, биотехнологов, биофизиков, вирусологов и молекулярных биологов — 2023. Novosibirsk State University, 2023. http://dx.doi.org/10.25205/978-5-4437-1526-1-169.

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We studied the interaction of the NM domain of the influenza A virus M1 protein with lipid membranes and the structure of the protein layer formed by it. The binding constant of this domain to the membrane was determined; its value turned out to be close to that for the full-length M1 protein. This confirms the hypothesis that the NM domain determines the interaction of the protein with the membrane and can be used as a target for the search for antiviral drugs.
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Emami, Babak, Rui Liu, David S. K. Ting, and M. David Checkel. "Rapid Distortion of Homogeneous Turbulence Between Cylinders." In ASME 2004 International Mechanical Engineering Congress and Exposition. ASMEDC, 2004. http://dx.doi.org/10.1115/imece2004-60125.

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The effects of ‘half-full-half’ cylinders on the distortion of a turbulent flow are experimentally investigated. A single hot-wire anemometry unit is used to measure the stream-wise parameters of the flow. The cylinders are 15.2 cm (6 in) in diameter and 76.2 cm (30 in) in height installed in a closed loop wind tunnel with a 76.2 cm by 76.2 cm (30 in by 30 in) cross section. Turbulent flow with a nominal mean velocity of 7.6 m/s was generated by means of a perforated plate situated at the entrance of the wind tunnel. It was found that the mean velocity increases significantly as the flow passe
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Kristensen, Claus E., Jan Muren, Andreas Gjendal, et al. "Full-Scale Validation of Axial Carcass Loads in Flexible Pipe Structure From Cyclic Pressure and Temperature." In ASME 2017 36th International Conference on Ocean, Offshore and Arctic Engineering. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/omae2017-62042.

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With basis in field experiences from several carcass axial failures in HT/HP unbonded flexible risers a comprehensive program was initiated to determine the root cause and develop a theoretical framework for prediction of the loads and mechanisms leading to damage initiation and development. The physical understanding, theory, tools and methods were developed in a close cooperation between the field operator Statoil, 4Subsea and DNV GL. Following the discovery of carcass tearing as a new failure mode in May 2011 substantial work has been put into small- and mid-scale testing together with the
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Reports on the topic "Full-length clone"

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Loebenstein, Gad, William Dawson, and Abed Gera. Association of the IVR Gene with Virus Localization and Resistance. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7604922.bard.

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We have reported that localization of TMV in tobacco cultivars with the N gene, is associated with a 23 K protein (IVR) that inhibited replication of several plant viruses. This protein was also found in induced resistant tissue of Nicotiana glutinosa x Nicotiana debneyi. During the present grant we found that TMV production is enhanced in protoplasts and plants of local lesion responding tobacco cultivars exposed to 35oC, parallel to an almost complete suppression of the production of IVR. We also found that IVR is associated with resistance mechanisms in pepper cultivars. We succeeded to clo
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Gafny, Ron, A. L. N. Rao, and Edna Tanne. Etiology of the Rugose Wood Disease of Grapevine and Molecular Study of the Associated Trichoviruses. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7575269.bard.

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Rugose wood is a complex disease of grapevines, characterized by modification of the woody cylinder of affected vines. The control of rugose wood is based on the production of healthy propagation material. Detection of rugose wood in grapevines is difficult and expensive: budwood from tested plants is grafted onto sensitive Vitis indicators and the appearance of symptoms is monitored for 3 years. The etiology of rugose wood is complex and has not yet been elucidated. Several elongated clostero-like viruses are consistently found in affected vines; one of them, grapevine virus A (GVA), is close
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DeMartini, James C., Abraham Yaniv, Jonathan O. Carlson, et al. Evaluation of Naked Proviral DNA as a Vaccine for Ovine Lentivirus Infection. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7570553.bard.

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Ovine lentivirus (OvLV) infection is widespread in sheep of the United States and Israel and is responsible for substantial economic losses. The primary goal of this project was to evaluate naked proviral DNA as a vaccine to induce protective immunity in sheep in endemic areas. Contrary to expectations, inoculation of sheep with proviral DNA derived from the full length OvLV molecular clone pkv72 did not result in detectable OvLV infection, but infectious virus was recovered from transfected ovine cells. Kv72 virus produced by these cells infected sheep and induced antibody responses, and was
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Mawassi, Munir, Baozhong Meng, and Lorne Stobbs. Development of Virus Induced Gene Silencing Tools for Functional Genomics in Grapevine. United States Department of Agriculture, 2013. http://dx.doi.org/10.32747/2013.7613887.bard.

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Grapevine is perhaps the most widely grown fruit crop. To understand the genetic make-up so as to improve the yield and quality of grapes and grape products, researchers in Europe have recently sequenced the genomes of Pinot noir and its inbred. As expected, function of many grape genes is unknown. Functional genomics studies have become the major focus of grape researchers and breeders. Current genetic approaches for gene function studies include mutagenesis, crossing and genetic transformation. However, these approaches are difficult to apply to grapes and takes long periods of time to accom
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Sooksai, Sarintip, and Kobkul Laoteng. Genetic study of fatty acid and lipid biosynthesis in Hansenula polymorpha. Chulalongkorn University, 2011. https://doi.org/10.58837/chula.res.2011.121.

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Two groups of Hansenula polymorpha mutants were screened by their fatty acid requirement, one requires saturated fatty acids for growth (sfa- mutamts) and the other requires unsaturated fatty acids (mfa- and pfa- mutants). Two of the sfa- mutants, S7 and S16, showed significantly difference in the fatty acid composition. S7 clearly defected in the production of C18:2∆9,12 and C18:3∆9,12,15, while S16 significantly accumulated medium-chain saturated fatty acids, C12:0 and C14:0. By tetrad analysis, the results showed that S7 had double mutation which composed of fatty acid synthesis mutation (H
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Funkenstein, Bruria, and Shaojun (Jim) Du. Interactions Between the GH-IGF axis and Myostatin in Regulating Muscle Growth in Sparus aurata. United States Department of Agriculture, 2009. http://dx.doi.org/10.32747/2009.7696530.bard.

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Growth rate of cultured fish from hatching to commercial size is a major factor in the success of aquaculture. The normal stimulus for muscle growth in growing fish is not well understood and understanding the regulation of muscle growth in fish is of particular importance for aquaculture. Fish meat constitutes mostly of skeletal muscles and provides high value proteins in most people's diet. Unlike mammals, fish continue to grow throughout their lives, although the size fish attain, as adults, is species specific. Evidence indicates that muscle growth is regulated positively and negatively by
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Rafaeli, Ada, Russell Jurenka, and Daniel Segal. Isolation, Purification and Sequence Determination of Pheromonotropic-Receptors. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7695850.bard.

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Moths constitute a major group of pest insects in agriculture. Pheromone blends are utilised by a variety of moth species to attract conspecific mates, which is under circadian control by the neurohormone, PBAN (pheromone-biosynthesis-activating neuropeptide). Our working hypothesis was that, since the emission of sex-pheromone is necessary to attract a mate, then failure to produce and emit pheromone is a potential strategy for manipulating adult moth behavior. The project aimed at identifying, characterising and determining the sequence of specific receptors responsible for the interaction w
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Bar-Joseph, Moshe, William O. Dawson, and Munir Mawassi. Role of Defective RNAs in Citrus Tristeza Virus Diseases. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7575279.bard.

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This program focused on citrus tristeza virus (CTV), the largest and one of the most complex RNA-plant-viruses. The economic importance of this virus to the US and Israeli citrus industries, its uniqueness among RNA viruses and the possibility to tame the virus and eventually turn it into a useful tool for the protection and genetic improvement of citrus trees justify these continued efforts. Although the overall goal of this project was to study the role(s) of CTV associated defective (d)-RNAs in CTV-induced diseases, considerable research efforts had to be devoted to the engineering of the h
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Friedman, Haya, Julia Vrebalov, James Giovannoni, and Edna Pesis. Unravelling the Mode of Action of Ripening-Specific MADS-box Genes for Development of Tools to Improve Banana Fruit Shelf-life and Quality. United States Department of Agriculture, 2010. http://dx.doi.org/10.32747/2010.7592116.bard.

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Fruit deterioration is a consequence of a genetically-determined fruit ripening and senescence programs, in which developmental factors lead to a climacteric rise of ethylene production in ethylene-sensitive fruits such as tomato and banana. Breeding of tomato with extended fruit shelf life involves the incorporation of a mutation in RIN, a MADS-box transcription factor participating in developmental control signalling of ripening. The RIN mode of action is not fully understood, and it may be predicted to interact with other MADS-box genes to execute its effects. The overall goal of this study
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