Relevant bibliographies by topics / Fusarium wilt of banana

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Academic literature on the topic 'Fusarium wilt of banana'

Author: Grafiati
Published: 4 June 2021
Last updated: 30 January 2023

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Journal articles on the topic "Fusarium wilt of banana"

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Mariana, Mariana, and Ismed Setya Budi. "RESISTANCE OF LOCAL BANANA VARIETIES TO FUSARIUM WILT DISEASE." TROPICAL WETLAND JOURNAL 3, no. 1 (March 21, 2017): 31–35. http://dx.doi.org/10.20527/twj.v3i1.44.

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Fusarium wilt is one of the important banana diseases that lead to low quality and quantity of banana production. The objective of this study was to examine and study the resistance of some local banana varieties to fusarium wilt. Pathogens were isolated from the weevil of kepok banana (saba banana) that showed severe symptoms of Fusarium wilt. The inoculum was prepared by propagating the pathogens on Potato Dextrose Agar (PDA) medium. Inoculation was carried out using mycelium fragment suspension (12 mg hyphae mL-1 tween 20 0.05%). The roots of the two-week Ambon banana were injured and immersed in the suspension. Pathogens were determined using Koch's postulates. The varieties tested were seven (7) local banana varieties grown in South Kalimantan. Levels of resistance were determined based on the scale of Leaf Symptom Index (LSI). The pathogens were identified as Fusarium oxysporum f.sp. cubense. The results showed that there were differences in the levels of resistance; Kepok banana (highly susceptible); yellow Ambon, Awa, Susu, Mahuli and Talas bananas (susceptible), and Manggala banana (tolerant).
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Ploetz, Randy C. "Fusarium Wilt of Banana." Phytopathology® 105, no. 12 (December 2015): 1512–21. http://dx.doi.org/10.1094/phyto-04-15-0101-rvw.

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Banana (Musa spp.) is one of the world’s most important fruits. In 2011, 145 million metric tons, worth an estimated $44 billion, were produced in over 130 countries. Fusarium wilt (also known as Panama disease) is one of the most destructive diseases of this crop. It devastated the ‘Gros Michel’-based export trades before the mid-1900s, and threatens the Cavendish cultivars that were used to replace it; in total, the latter cultivars are now responsible for approximately 45% of all production. An overview of the disease and its causal agent, Fusarium oxysporum f. sp. cubense, is presented below. Despite a substantial positive literature on biological, chemical, or cultural measures, management is largely restricted to excluding F. oxysporum f. sp. cubense from noninfested areas and using resistant cultivars where the pathogen has established. Resistance to Fusarium wilt is poor in several breeding targets, including important dessert and cooking cultivars. Better resistance to this and other diseases is needed. The history and impact of Fusarium wilt is summarized with an emphasis on tropical race 4 (TR4), a ‘Cavendish’-killing variant of the pathogen that has spread dramatically in the Eastern Hemisphere.
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Pegg, KG, NY Moore, and S. Bentley. "Fusarium wilt of banana in Australia: a review." Australian Journal of Agricultural Research 47, no. 5 (1996): 637. http://dx.doi.org/10.1071/ar9960637.

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The history of Fusarium wilt of bananas (caused by F. oxysporum f.sp. cubense) and the research conducted on the disease in Australia is summarised in this review. Subjects covered include the classification of banana cultivars, the cultivars in production in the Australian banana industry, the distribution and diversity of the pathogen in Australia and pathogenicity and control of the disease.
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Le Thi, Loan, Arne Mertens, Dang Toan Vu, Tuong Dang Vu, Pham Le Anh Minh, Huy Nguyen Duc, Sander de Backer, et al. "Diversity of Fusarium associated banana wilt in northern Viet Nam." MycoKeys 87 (February 10, 2022): 53–76. http://dx.doi.org/10.3897/mycokeys.87.72941.

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Fusarium is one of the most important fungal genera of plant pathogens that affect the cultivation of a wide range of crops. Agricultural losses caused by Fusarium oxysporum f. sp. cubense (Foc) directly affect the income, subsistence, and nourishment of thousands of farmers worldwide. For Viet Nam, predictions on the impact of Foc for the future are dramatic, with an estimated loss in the banana production area of 8% within the next five years and up to 71% within the next 25 years. In the current study, we applied a combined morphological-molecular approach to assess the taxonomic identity and phylogenetic position of the different Foc isolates collected in northern Viet Nam. In addition, we aimed to estimate the proportion of the different Fusarium races infecting bananas in northern Viet Nam. The morphology of the isolates was investigated by growing the collected Fusarium isolates on four distinct nutritious media (PDA, SNA, CLA, and OMA). Molecular phylogenetic relationships were inferred by sequencing partial rpb1, rpb2, and tef1a genes and adding the obtained sequences into a phylogenetic framework. Molecular characterization shows that c. 74% of the Fusarium isolates obtained from infected banana pseudostem tissue belong to F. tardichlamydosporum. Compared to F. tardichlamydosporum, F. odoratissimum accounts for c.10% of the Fusarium wilt in northern Viet Nam, demonstrating that Foc TR4 is not yet a dominant strain in the region. Fusarium cugenangense – considered to cause Race 2 infections among bananas – is only found in c. 10% of the tissue material that was obtained from infected Vietnamese bananas. Additionally, one of the isolates cultured from diseased bananas was phylogenetically not positioned within the F. oxysporum species complex (FOSC), but in contrast, fell within the Fusarium fujikuroi species complex (FFSC). As a result, a possible new pathogen for bananas may have been found. Besides being present on several ABB ‘Tay banana’, F. tardichlamydosporum was also derived from infected tissue of a wild Musa lutea, showing the importance of wild bananas as a possible sink for Foc.
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BUKHARI, BUKHARI, and NURYULSEN SAFRIDAR. "PENGARUH PEMBERIAN Trichoderma Sp UNTUK MENGENDALIKAN PENYAKIT LAYU FUSARIUM PADA BEBERAPA JENIS PISANG DI LAHAN YANG TELAH TERINFEKSI." Jurnal Ilmiah Pertanian 15, no. 1 (August 1, 2018): 23–34. http://dx.doi.org/10.31849/jip.v15i1.1480.

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The experimental design used was a 4x4 factorial with 2 replications in a randomized block design (RAK) pattern. The first factor is the Type of Banana (J) consisting of 4 levels: J1 (Raja Banana ), J2 (Ambon Banana), J3 (Barangan Banana) and J4 (Geupok Banana). The second factor was the mixing of Trichoderma Sp with organic manure aplication (D) consisting of 4 levels: D0 (without trichoderma sp), D1 (200 g trichoderma sp in 4 kg manure), D2 (200 gr trichoderma sp in 8 kg manure), and D3 (200 gr trichoderma sp in 12 kg manure). Observations made include: Intensity of Fusarium oxysporum wilting attack, number of leaves, Leaf length and stem diameeter. The results showed that the type of banana had a very significant effect on the intensity of fusarium oxysporum wilt disease and the number of leaves, but no significant effect on the length and diameter of the stem. Barangan Bananas and Ambon Bananas show stronger resilience (more resistant to fusarium oxysporum wilt disease). While the Raja Banana and Geupok Bananas look more sensitive to the attack of the disease. The mixing of Trichoderma Sp with organic manure also significantly influenced the intensity of fusarium oxysporum wilt disease, number of banana leaf and leaf length, but no significant effect on stem diameter. The best dose is found in treatment D1, but not different from D2. An increase in the amount of organic matter manure
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Tanjung, Mei Rani, Abdul Munif, Yunus Effendi, and Efi Toding Tondok. "The Severity of Fusarium Wilt Disease in Correlation to the Abundance of Fusarium oxysporum and Phytonematodes: Case Study at Banana Plantation PTPN VIII Parakansalak." Jurnal Fitopatologi Indonesia 18, no. 5 (January 3, 2023): 222–30. http://dx.doi.org/10.14692/jfi.18.5.222-230.

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The Severity of Fusarium Wilt Disease in Correlation to the Abundance of Fusarium oxysporum and Phytonematodes: Case Study at Banana Plantation PTPN VIII Parakansalak Fusarium oxysporum f. sp. cubense is a soil-borne pathogen that infects banana plants and causes wilt. Several studies demonstrated that phytomatodes have a role in helping infection of the pathogens that cause wilt. This study aims to determine the relationship between phytonematodes and Fusarium oxysporum f. sp. cubense from soil and banana roots infected with fusarium wilt. Soil samples was obtained from infected banana plants and collected based on different scores of fusarium wilt disease severity. Samples were then composited based on the score, and the abundance of each pathogen is measured following samples extraction in the laboratory. The highest population of phytonematodes was 77 g-1 obtained from soil with a disease severity score of 4; and the lowest was 16 g-1 from samples with a score of 2. As for root samples, the highest population of phytonematodes (85 g-1) was obtained from plants with a score 0 and the lowest (33 g-1) was from plants with a score 3. Two species of phytonematodes were identified, i.e. Helicotylenchus sp. and Radopolus sp. Based on measurement using conventional methods showed that the abundance of Fusarium oxysporum f. sp. cubense was highest in plants with a score of 1, i.e. 8.1 × 103 cfu g-1 soil and lowest in plants with a score of 0 i.e. 1.0 × 103 cfu g-1 soil. The effect of Fusarium oxysporum f. sp. cubense to the severity of fusarium wilt shows a positive relationship of 8.3% and it may contribute to cause fusarium wilt disease by 0.6%. This pathogen can cause wilting of bananas without the help of the phytonematode species found in this study.
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Khan, Babar, Zuniara Akash, Shahzad Asad, Nazir Javed, Nasir A. Rajput, Abdul Jabbar, Wasi U. Din, and Rana M. Atif. "ANTAGONISTIC POTENTIAL OF TRICHODERMA HARZIANUM AGAINST FUSARIUM OXYSPORUM F. SP. CUBENSE ASSOCIATED WITH PANAMA WILT OF BANANA." Pakistan Journal of Phytopathology 29, no. 1 (July 12, 2017): 111. http://dx.doi.org/10.33866/phytopathol.029.01.0299.

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Fusarium oxysporum f. sp. cubense is a soil loving pathogen of banana that can cause distortion of vascular system. It is responsible for the disease Fusarium wilt of bananas also renowned as panama wilt disease that has responsible of immense losses in the banana industry worldwide. In this study, diseased samples were collected from rhizosphere of banana plants in the research area of National Agriculture Research Centre (NARC), Islamabad and cultured on PDA to isolate pathogenic strains of F. oxysporium f. sp. cubense. Antagonistic potential of Trichoderma harzianum against F. oxysporum f. sp. cubense was evaluated under controlled conditions. T. harzianum produced up to 75.5% inhibition of colony growth of the pathogen followed by incubation for 72 h at 28+2°C in vitro. In pot culture T. harzianum considerably reduced disease severity. It proves that it is a potential biological control agent against banana wilt pathogen.
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Deng, Xiao, Qin Fen Li, Xian Wen Hou, and Chun Yuan Wu. "Soil Microbial Functional Diversity from Different Infection Grades of Banana Fusarium Wilt (Fusarium oxysporum f. sp. Cubense)." Applied Mechanics and Materials 295-298 (February 2013): 2274–80. http://dx.doi.org/10.4028/www.scientific.net/amm.295-298.2274.

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Thirty rhizosphere and non-rhizosphere soil samples from different infection grades(0, I, III, V and VII) of three typical banana plots(Jianfeng, Shiyuetian, Chongpo) infected by banana fusarium wilt (Fusarium oxysporum f. sp. cubense) in Hainan province were collected to study the microbial community functional diversity applying Biolog-ECO microplates technology. The results are as follows: (1) Overall carbon source metabolic capacities of soil microbial community weaken with increasing of infection grades of banana fusarium wilt. (2) Richness indices, Simpson indices, Shannon indices and McIntosh indices of soil microbial community gradually decreased with increasing of infection grades of banana fusarium wilt. (3) Principal component analysis show that metabolic characteristics of soil microbial community significantly change between the healthy plants and diseased plants in the same banana plot. The results would provide information for explaining the pathogenesis of banana fusarium wilt and controlling its incidence by applying microbial ecology to regulate soil environmental measures.
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Bukhari, Bukhari, and Nuryulsen Safridar. "EFISIENSI PENGGUNAAN Trichoderma sp UNTUK MENGENDALIKAN PENYAKIT LAYU FUSARIUM (Fusarium oxysporium) DAN PERTUMBUHAN BIBIT TANAMAN PISANG." Jurnal Ilmiah Pertanian 14, no. 2 (March 15, 2018): 14–28. http://dx.doi.org/10.31849/jip.v14i2.256.

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This research was conducted in Pante Cermin, Padang Tiji Subdistrict, Pidie District. in March to August 2015, with the aim to know the use efficienci of Trichoderma sp on the growth of several types of Banana seedlings (Musa Paracica L.) This research using Randomized Block Design (RAK) factorial pattern with the factors studied is the type of banana and trichoderma dose . Both factors consist of 4 levels: Banana Barangan (J1), Ambon banana (J2), Banana King (J3) and Geupok banana (J4). While the dose of Tricoderma sp is: TO = 0 g / seeds T1 = 15 g / seeds T2 = 30 g / seedlings and T3 = 45 g / seedlings. So there are 16 treatment combinations and repeated 3 times, which resulted in 48 experimental units.To determine the influence of each treatment and its interaction on the growth of banana seedlings, the analysis of variance (Test F) and continued with the test of Beda Nyata Jujur (BNJ) at 5% level.The results showed that the growth of good banana seedlings among 4 species studied was shown by Barangan bananas, but not unlike bananas. However, trichoderma administration until the age of 4 months has not shown the growth and intensity of different attacks. So it should be extended the study period to 6 months. After 6 months of research there has been a difference in growth and intensity of attack, where the intensity of the greatest attack is shown by T0 (without trichoderma). Medium intensity of smallest attack is shown by T3 (Dose trichoderma sp 45 gr / banana seedlings). The type of banana does not affect the growth and intensity of fusarium wilt attack. and the interaction of these two factors had no significant effect on the growth of banana seedlings and the intensity of Fusarium wilt disease.
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Nasir, Nasril, P. A. Pittaway, K. G. Pegg, and A. T. Lisle. "A pilot study investigating the complexity of Fusarium wilt of bananas in West Sumatra, Indonesia." Australian Journal of Agricultural Research 50, no. 7 (1999): 1279. http://dx.doi.org/10.1071/ar97079.

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Fusarium wilt symptoms were observed on 15 different banana cultivars growing in commercial plantations and backyard holdings, in 6 districts in the Province of West Sumatra, Indonesia. Affected cultivars included the most popular dessert bananas Pisang buai (AAA), Pisang raja (AAB), Pisang raja serai (AAB), and Pisang ambon (AAA). Disease symptoms were also observed on the most popular cooking banana Pisang kepok (ABB), formerly considered to be resistant. However, no disease symptoms were observed on wild Musa species. The 37 isolates of the pathogen Fusarium oxysporum f. sp. cubense (Foc) recovered from the vascular tissue of the diseased bananas were assigned to the vegetative compatability groups (VCGs) 0120–01215, 0124, 01213–01216, 01218, and 01219. VCG 01216 was isolated from all districts, and from 14 of the 15 cultivars exhibiting wilt symptoms. However, the distribution of the other VCGs was more variable, reflecting the patterns of human migration within the different districts. Districts designated as expanding urban centres or as transmigration centres had a higher diversity of banana genotypes present, with a correspondingly higher diversity of VCGs isolated from the diseased plants. The traditional practice of transplanting banana suckers and rhizomes irrespective of the disease status of the parent plant, and the recent increase in the mobility of the Indonesian population, indicate that disease control strategies based on quarantine will not be effective. Accordingly, the selection of both popular dessert and cooking banana cultivars with resistance to VCG 01216 should be a priority for the control of Fusarium wilt in West Sumatra.
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Dissertations / Theses on the topic "Fusarium wilt of banana"

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Visser, Marinda. "Molecular biological studies of the Fusarium wilt pathogen of banana in South Africa." Thesis, Pretoria [s.n.], 2003. http://upetd.up.ac.za/thesis/available/etd-04042005-144251.

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Nasir, Nasril. "The chicken manure assay as a potential screening technique to select banana cultivars with field resistance to Panama disease /." [St. Lucia, Qld.], 2002. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe16269.pdf.

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Sorenson, Suzanne. "Genetic variation within Fusarium Oxysporum f.sp. cubense in Banana." Thesis, Queensland University of Technology, 1993. https://eprints.qut.edu.au/105698/1/T%28S%29%2038%20Genetic%20variation%20within%20Fusarium%20oxysportum%20f.sp.%20cubanese%20in%20Banana.pdf.

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Genetic variation within the fungus F. oxysporum f.sp. cubense, which causes Fusarium wilt in banana, was examined using RAPD-PCR. RAPDPCR is a method of generating genome specific "fingerprints" which can be compared to determine genetic relatedness. In this study, the RAPD-PCR technique was optimised and used to determine the genetic relatedness between different vegetative compatibility groups (VCGs) and races, and among isolates within different VCGs and races of F. oxysporum f.sp. cubense. An understanding of the genetic diversity within F. oxysporum f.sp. cubense is essential to select or breed banana cultivars with durable resistance to Fusarium wilt; potentially resistant cultivars ideally should have resistance to all variants of the pathogen. Investigating genetic variation within the pathogen may also provide insight into the evolution of different populations and pathotypes of F. oxysporum f.sp. cubense.
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Munro, Claire Louise. "Identification of defence-related genes in banana against Fusarium Wilt." Diss., University of Pretoria, 2008. http://hdl.handle.net/2263/29548.

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Bananas (Musa sp.) serve as a staple diet and source of income for millions of people worldwide. The crop, however, is vulnerable to several important diseases such as Fusarium wilt, caused by the soilborne fungus Fusarium oxysporum f.sp. cubense (Foc) . During the mid-20th century, thousands of acres of export banana plantations were lost in Central America due to Fusarium wilt. The epidemic was brought under control only by replacing the highly susceptible Gros Michel banana with Cavendish cultivars. Cavendish bananas, however, are susceptible to Foe race 4, a highly virulent variant of the pathogen that also causes disease to banana varieties susceptible to Foc races 1 and 2. Only this time, no resistant replacement varieties acceptable to the commercial market exist, while cultural and/or chemical control strategies proved to be ineffective for disease control. The only sustainable solution, thus, would be the improvement of existing banana varieties that are acceptable to consumers for resistance to Fusarium wilt. An understanding of how plants defend themselves against pathogens is an imperative first step towards the development of disease-resistant plants. Two broad defence mechanisms against pathogen attack exist in plants: Constitutive defence mechanisms that are pathogen non-specific and induced plant defence responses following recognition of specific pathogen features. A primary objective of this study was to elucidate induced banana defence responses. Induced defence responses may include the induction of regulator and antifungal proteins, the production of active-oxygen species or products from the phenylpropanoid pathway. A few studies have attempted to elucidate the genetic factors involved in the banana defence response but to date no clear answer has arisen. Forward and reverse genetics encompass approached by which plant defence responses can be studied; a particular technique of interest which can be classified under both forward and reverse genetics is cDNA-Amplified Fragment Length Polymorphism (AFLP) In a field trial conducted in an Foc-infested field in Kiepersol, South Africa, cultivars Rose and Calcutta IV proved resistant, and FHIA-17 tolerant to Foe 'subtropical' race 4 (VCG 0120) when compared to the highly susceptible Cavendish cultivar 'Williams'. The genetic basis of defence to Foc in FHIA-17, Rose and Calcutta IV was verified using quantitative real-time reverse transcriptase PCR (qRT-RT-PCR). Catalase, POX and PAE were strongly up-regulated in the tolerant and resistant banana varieties. These genes are involved in the oxidative burst and secondary metabolism leading to the phenylpropanoid pathway and cell wall strengthening. Resistance to Foc, 'subtropical' race 4, thus seems to depend on the early recognition of the pathogen and subsequent blocking of its progress into the plant's vascular system. The response of resistant and tolerant banana varieties to Foc was elucidated by analysing the banana transcriptome 6 and 72 hours post inoculation (hpi) using cDNA-AFLP. Seventy-six differentially expressed transcript derived fragments (TDFs) were isolated, sequenced and subjected to BLASTX and BLASTN searches. Many of the sequences were not significantly similar to any other sequences in the databases, but several genes fragments showed homology to defence-related genes. TDFs representing genes such as S-adenosylmethionine synthase (SAMS) and isoflavone reductase, which are potentially involved in the production of cell wall strengthening compounds such as lignin, were identified. Expression patterns of selected TDFs as seen on the cDNA-AFLP gels were confirmed using qRT -PCR. As additional endogenous controls, two TDFs which displayed constitutive expression on the cDNA-AFLP gels, were isolated, sequenced and were optimised for use as endogenous control genes for the normalisation of the qRT-PCR data. In most cases, the expression patterns seen on the cDNA-AFLP gels were replicated by qRT-PCR. This study concludes that the tolerant (FHIA-17) and resistant (Rose and Calcutta IV) banana varieties induced defence-related genes upon attack by Foc and that the cDNA-AFLP technique was further effective in identifying additional defence-related genes. Looking to the future, the greatest understanding of the defence responses induced during the banana/Foe interaction would be obtained by using additional molecular approaches or techniques. It is in some cases sufficient to look exclusively at transcriptomic i.e. cDNA-AFLP and microarray data to study the plant's response, however, a look at differential protein and metabolite expression would complement transcriptomic data and add insight into the fate of certain expressed genes. This would possibly speed up the identification of the defence pathways used by resistant bananas to resist infection by Foc, once identified these pathways can be manipulated in the susceptible plants and thus the generation of a Foc resistant banana could finally become a reality.
Dissertation (MSc)--University of Pretoria, 2011.
Microbiology and Plant Pathology
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Magambo, Betty. "Generating transgenic banana (cv. Sukali Ndizi) resistant to Fusarium Wilt." Thesis, Queensland University of Technology, 2012. https://eprints.qut.edu.au/61024/1/Betty_Magambo_Thesis.pdf.

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Banana is one of the world’s most popular fruit crops and Sukali Ndizi is the most popular dessert banana in the East African region. Like other banana cultivars, Sukali Ndizi is threatened by several constraints, of which the Fusarium wilt disease is the most destructive. Fusarium wilt is caused by a soil-borne fungus, Fusarium oxysporum f.sp. cubense (Foc). No effective control strategy currently exists for this disease and although disease resistance exists in some banana cultivars, introducing resistance into commercial cultivars by conventional breeding is difficult because of low fertility. Considering that conventional breeding generates hybrids with additional undesirable traits, transformation is the most suitable way of introducing resistance in the banana genome. The success of this strategy depends on the availability of genes for genetic transformation. Recently, a novel strategy involving the expression of anti-apoptosis genes in plants was shown to result in resistance against several necrotrophic fungi, including Foc race 1 in banana cultivar Lady Finger. This thesis explores the potential of a plant-codon optimised nematode anti-apoptosis gene (Mced9) to provide resistance against Foc race 1 in dessert banana cultivar Sukali Ndizi. Agrobacterium-mediated transformation was used to transform embryogenic cell suspension of Sukali Ndizi with plant expression vector pYC11, harbouring maize ubiquitin promoter driven Mced9 gene and nptII as a plant selection marker. A total of 42 independently transformed lines were regenerated and characterized. The transgenic lines were multiplied, infected and evaluated for resistance to Foc race 1 in a small pot bioassay. The pathogenicity of the Ugandan Foc race 1 isolate used for infection was pre-determined and the spore concentration was standardised for consistent infection and symptom development. This process involved challenging tissue culture plants of Sukali Ndizi, a Foc race 1 susceptible cultivar and Nakinyika, an East African Highland cultivar known to be resistant to Foc race 1, with Fusarium inoculum and observing external and internal disease symptom development. Rhizome discolouration symptoms were the best indicators of Fusarium wilt with yellowing being an early sign of disease. Three transgenic lines were found to show significantly less disease severities compared to the wild-type control plants after 13 weeks of infection, indicating that Mced9 has the potential to provide tolerance to Fusarium wilt in Sukali Ndizi.
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Van, den Berg Noëlani. "Identification of genes associated with tolerance in the C Cavendish banana selection, GCTCV 218, against Fusarium oxysporum f.sp. cubense 'subtropical' race 4." Pretoria : [s.n.], 2006. http://upetd.up.ac.za/thesis/available/etd-11082006-171800.

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Groenewald, Susan. "Biology, pathogenicity and diversity of Fusarium oxysporum f.sp. cubense." Pretoria : [s.n.], 2005. http://upetd.up.ac.za/thesis/available/etd-02232007-175712.

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Namukwaya, Betty. "Evaluation of transgenic bananas expressing anti-apoptotic genes for resistance against Fusarium wilt." Thesis, Queensland University of Technology, 2015. https://eprints.qut.edu.au/91393/1/Betty_Namukwaya_Thesis.pdf.

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The banana industry worldwide is under threat from a fungal disease known as Fusarium wilt, a disease for which there is no chemical control. Conventional breeding approaches to generate resistant banana varieties are lengthy and very difficult. As such, genetic engineering for disease resistance is considered the most viable control option. In this PhD thesis, genetically modified banana plants were generated using several different stress tolerance genes. When challenged with Fusarium wilt in glasshouse trials, some lines showed increased resistance to the disease. The promising elite lines generated in this study will now require testing in field trials.
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Paul, Jean-Yves. "The manipulation of apoptosis-related genes to generate resistance to Fusarium wilt and water stress in banana." Thesis, Queensland University of Technology, 2009. https://eprints.qut.edu.au/29263/1/Jean-Yves_Paul_Citation.pdf.

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Bananas are susceptible to a diverse range of biotic and abiotic stresses, many of which cause serious production constraints worldwide. One of the most destructive banana diseases is Fusarium wilt caused by the soil-borne fungus, Fusarium oxysporum f. sp. cubense (Foc). No effective control strategy currently exists for this disease which threatens global banana production. Although disease resistance exists in some wild bananas, attempts to introduce resistance into commercially acceptable bananas by conventional breeding have been hampered by low fertility, long generation times and association of poor agronomical traits with resistance genes. With the advent of reliable banana transformation protocols, molecular breeding is now regarded as a viable alternative strategy to generate disease-resistant banana plants. Recently, a novel strategy involving the expression of anti-apoptosis genes in plants was shown to result in resistance against several necrotrophic fungi. Further, the transgenic plants showed increased resistance to a range of abiotic stresses. In this thesis, the use of anti-apoptosis genes to generate transgenic banana plants with resistance to Fusarium wilt was investigated. Since water stress is an important abiotic constraint to banana production, the resistance of the transgenic plants to water stress was also examined. Embryogenic cell suspensions (ECS) of two commercially important banana cultivars, Grand Naine (GN) and Lady Finger (LF), were transformed using Agrobacterium with the anti-apoptosis genes, Bcl-xL, Bcl-xL G138A, Ced-9 and Bcl- 2 3’ UTR. An interesting, and potentially important, outcome was that the use of anti-apoptosis genes resulted in up to a 50-fold increase in Agrobacterium-mediated transformation efficiency of both LF and GN cells over vector controls. Regenerated plants were subjected to a complete molecular characterisation in order to detect the presence of the transgene (PCR), transcript (RT-PCR) and gene product (Western blot) and to determine the gene copy number (Southern blot). A total of 36 independently-transformed GN lines (8 x Bcl-xL, 5 x Bcl-xL G138A, 15 x Ced-9 and 8 x Bcl-2 3’ UTR) and 41 independently-transformed LF lines (8 x Bcl-xL, 7 x BclxL G138A, 13 x Ced-9 and 13 x Bcl-2 3’ UTR) were identified. The 41 transgenic LF lines were multiplied and clones from each line were acclimatised and grown under glasshouse conditions for 8 weeks to allow monitoring for phenotypic abnormalities. Plants derived from 3 x Bcl-xL, 2 x Ced-9 and 5 x Bcl-2 3’ UTR lines displayed a variety of aberrant phenotypes. However, all but one of these abnormalities were off-types commonly observed in tissue-cultured, non-transgenic banana plants and were therefore unlikely to be transgene-related. Prior to determining the resistance of the transgenic plants to Foc race 1, the apoptotic effects of the fungus on both wild-type and Bcl-2 3’ UTR-transgenic LF banana cells were investigated using rapid in vitro root assays. The results from these assays showed that apoptotic-like cell death was elicited in wild-type banana root cells as early as 6 hours post-exposure to fungal spores. In contrast, these effects were attenuated in the root cells of Bcl-2 3’ UTR-transgenic lines that were exposed to fungal spores. Thirty eight of the 41 transgenic LF lines were subsequently assessed for resistance to Foc race 1 in small-plant glasshouse bioassays. To overcome inconsistencies in rating the internal (vascular discolouration) disease symptoms, a MatLab-based computer program was developed to accurately and reliably assess the level of vascular discolouration in banana corms. Of the transgenic LF banana lines challenged with Foc race 1, 2 x Bcl-xL, 3 x Ced-9, 2 x Bcl-2 3’ UTR and 1 x Bcl-xL G138A-transgenic line were found to show significantly less external and internal symptoms than wild-type LF banana plants used as susceptible controls at 12 weeks post-inoculation. Of these lines, Bcl-2 3’ UTR-transgenic line #6 appeared most resistant, displaying very mild symptoms similar to the wild-type Cavendish banana plants that were included as resistant controls. This line remained resistant for up to 23 weeks post-inoculation. Since anti-apoptosis genes have been shown to confer resistance to various abiotic stresses in other crops, the ability of these genes to confer resistance against water stress in banana was also investigated. Clonal plants derived from each of the 38 transgenic LF banana plants were subjected to water stress for a total of 32 days. Several different lines of transgenic plants transformed with either Bcl-xL, Bcl-xL G138A, Ced-9 or Bcl-2 3’ UTR showed a delay in visual water stress symptoms compared with the wild-type control plants. These plants all began producing new growth from the pseudostem following daily rewatering for one month. In an attempt to determine whether the protective effect of anti-apoptosis genes in transgenic banana plants was linked with reactive oxygen species (ROS)-associated programmed cell death (PCD), the effect of the chloroplast-targeting, ROS-inducing herbicide, Paraquat, on wild-type and transgenic LF was investigated. When leaf discs from wild-type LF banana plants were exposed to 10 ìM Paraquat, complete decolourisation occurred after 48 hours which was confirmed to be associated with cell death and ROS production by trypan blue and 3,3-diaminobenzidine (DAB) staining, respectively. When leaf discs from the transgenic lines were exposed to Paraquat, those derived from some lines showed a delay in decolourisation, suggesting only a weak protective effect from the transgenes. Finally, the protective effect of anti-apoptosis genes against juglone, a ROS-inducing phytotoxin produced by the causal agent of black Sigatoka, Mycosphaerella fijiensis, was investigated. When leaf discs from wild-type LF banana plants were exposed to 25 ppm juglone, complete decolourisation occurred after 48 hours which was again confirmed to be associated with cell death and ROS production by trypan blue and DAB staining, respectively. Further, TdT-mediated dUTP nick-end labelling (TUNEL) assays on these discs suggested that the cell death was apoptotic. When leaf discs from the transgenic lines were exposed to juglone, discs from some lines showed a clear delay in decolourisation, suggesting a protective effect. Whether these plants are resistant to black Sigatoka is unknown and will require future glasshouse and field trials. The work presented in this thesis provides the first report of the use of anti-apoptosis genes as a strategy to confer resistance to Fusarium wilt and water stress in a nongraminaceous monocot, banana. Such a strategy may be exploited to generate resistance to necrotrophic pathogens and abiotic stresses in other economically important crop plants.
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Paul, Jean-Yves. "The manipulation of apoptosis-related genes to generate resistance to Fusarium wilt and water stress in banana." Queensland University of Technology, 2009. http://eprints.qut.edu.au/29263/.

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Bananas are susceptible to a diverse range of biotic and abiotic stresses, many of which cause serious production constraints worldwide. One of the most destructive banana diseases is Fusarium wilt caused by the soil-borne fungus, Fusarium oxysporum f. sp. cubense (Foc). No effective control strategy currently exists for this disease which threatens global banana production. Although disease resistance exists in some wild bananas, attempts to introduce resistance into commercially acceptable bananas by conventional breeding have been hampered by low fertility, long generation times and association of poor agronomical traits with resistance genes. With the advent of reliable banana transformation protocols, molecular breeding is now regarded as a viable alternative strategy to generate disease-resistant banana plants. Recently, a novel strategy involving the expression of anti-apoptosis genes in plants was shown to result in resistance against several necrotrophic fungi. Further, the transgenic plants showed increased resistance to a range of abiotic stresses. In this thesis, the use of anti-apoptosis genes to generate transgenic banana plants with resistance to Fusarium wilt was investigated. Since water stress is an important abiotic constraint to banana production, the resistance of the transgenic plants to water stress was also examined. Embryogenic cell suspensions (ECS) of two commercially important banana cultivars, Grand Naine (GN) and Lady Finger (LF), were transformed using Agrobacterium with the anti-apoptosis genes, Bcl-xL, Bcl-xL G138A, Ced-9 and Bcl- 2 3’ UTR. An interesting, and potentially important, outcome was that the use of anti-apoptosis genes resulted in up to a 50-fold increase in Agrobacterium-mediated transformation efficiency of both LF and GN cells over vector controls. Regenerated plants were subjected to a complete molecular characterisation in order to detect the presence of the transgene (PCR), transcript (RT-PCR) and gene product (Western blot) and to determine the gene copy number (Southern blot). A total of 36 independently-transformed GN lines (8 x Bcl-xL, 5 x Bcl-xL G138A, 15 x Ced-9 and 8 x Bcl-2 3’ UTR) and 41 independently-transformed LF lines (8 x Bcl-xL, 7 x BclxL G138A, 13 x Ced-9 and 13 x Bcl-2 3’ UTR) were identified. The 41 transgenic LF lines were multiplied and clones from each line were acclimatised and grown under glasshouse conditions for 8 weeks to allow monitoring for phenotypic abnormalities. Plants derived from 3 x Bcl-xL, 2 x Ced-9 and 5 x Bcl-2 3’ UTR lines displayed a variety of aberrant phenotypes. However, all but one of these abnormalities were off-types commonly observed in tissue-cultured, non-transgenic banana plants and were therefore unlikely to be transgene-related. Prior to determining the resistance of the transgenic plants to Foc race 1, the apoptotic effects of the fungus on both wild-type and Bcl-2 3’ UTR-transgenic LF banana cells were investigated using rapid in vitro root assays. The results from these assays showed that apoptotic-like cell death was elicited in wild-type banana root cells as early as 6 hours post-exposure to fungal spores. In contrast, these effects were attenuated in the root cells of Bcl-2 3’ UTR-transgenic lines that were exposed to fungal spores. Thirty eight of the 41 transgenic LF lines were subsequently assessed for resistance to Foc race 1 in small-plant glasshouse bioassays. To overcome inconsistencies in rating the internal (vascular discolouration) disease symptoms, a MatLab-based computer program was developed to accurately and reliably assess the level of vascular discolouration in banana corms. Of the transgenic LF banana lines challenged with Foc race 1, 2 x Bcl-xL, 3 x Ced-9, 2 x Bcl-2 3’ UTR and 1 x Bcl-xL G138A-transgenic line were found to show significantly less external and internal symptoms than wild-type LF banana plants used as susceptible controls at 12 weeks post-inoculation. Of these lines, Bcl-2 3’ UTR-transgenic line #6 appeared most resistant, displaying very mild symptoms similar to the wild-type Cavendish banana plants that were included as resistant controls. This line remained resistant for up to 23 weeks post-inoculation. Since anti-apoptosis genes have been shown to confer resistance to various abiotic stresses in other crops, the ability of these genes to confer resistance against water stress in banana was also investigated. Clonal plants derived from each of the 38 transgenic LF banana plants were subjected to water stress for a total of 32 days. Several different lines of transgenic plants transformed with either Bcl-xL, Bcl-xL G138A, Ced-9 or Bcl-2 3’ UTR showed a delay in visual water stress symptoms compared with the wild-type control plants. These plants all began producing new growth from the pseudostem following daily rewatering for one month. In an attempt to determine whether the protective effect of anti-apoptosis genes in transgenic banana plants was linked with reactive oxygen species (ROS)-associated programmed cell death (PCD), the effect of the chloroplast-targeting, ROS-inducing herbicide, Paraquat, on wild-type and transgenic LF was investigated. When leaf discs from wild-type LF banana plants were exposed to 10 ìM Paraquat, complete decolourisation occurred after 48 hours which was confirmed to be associated with cell death and ROS production by trypan blue and 3,3-diaminobenzidine (DAB) staining, respectively. When leaf discs from the transgenic lines were exposed to Paraquat, those derived from some lines showed a delay in decolourisation, suggesting only a weak protective effect from the transgenes. Finally, the protective effect of anti-apoptosis genes against juglone, a ROS-inducing phytotoxin produced by the causal agent of black Sigatoka, Mycosphaerella fijiensis, was investigated. When leaf discs from wild-type LF banana plants were exposed to 25 ppm juglone, complete decolourisation occurred after 48 hours which was again confirmed to be associated with cell death and ROS production by trypan blue and DAB staining, respectively. Further, TdT-mediated dUTP nick-end labelling (TUNEL) assays on these discs suggested that the cell death was apoptotic. When leaf discs from the transgenic lines were exposed to juglone, discs from some lines showed a clear delay in decolourisation, suggesting a protective effect. Whether these plants are resistant to black Sigatoka is unknown and will require future glasshouse and field trials. The work presented in this thesis provides the first report of the use of anti-apoptosis genes as a strategy to confer resistance to Fusarium wilt and water stress in a nongraminaceous monocot, banana. Such a strategy may be exploited to generate resistance to necrotrophic pathogens and abiotic stresses in other economically important crop plants.
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Books on the topic "Fusarium wilt of banana"

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Gatsinzi, François. La maladie de Panama due à Fusarium oxysporum f. sp. cubense (E.F. Smith) Synder & Hansen au sein de la Communauté économique des pays des grands lacs (Burundi, Rwanda, Zaïre). Gitega, République du Burundi: Institut de recherche agronomique et zootechnique de la C.E.P.G.L., 1989.

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Coleman, Jeffrey, ed. Fusarium wilt. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-1795-3.

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Marquina, J. C. Tello. Fusarium oxysporum en los cultivos intensivos del litoral Mediterráneo de España. Madrid: Ministerio de Agricultura Pesca y Alimentación, Secretaría General Técnica, 1990.

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Steel, Christopher Charles. Host-pathogen interactions in fusarium wilt of tomato. Birmingham: University of Birmingham, 1986.

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LaMondia, James Arthur. New Fusarium wilt-resistant Connecticut broadleaf tobacco varieties. New Haven: Connecticut Agricultural Experiment Station, 1991.

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LaMondia, James Arthur. New Fusarium wilt-resistant Connecticut broadleaf tobacco varieties. New Haven: Connecticut Agricultural Experiment Station, 1991.

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LaMondia, James Arthur. Scantic, a new fusarium-wilt resistant broadleaf tobacco cultivar. New Haven: Connecticut Agricultural Experiment Station, 2001.

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Whitehead, Debra Sian. Races and pathotypes of the wilt pathogen fusarium oxysporum. Norwich: University of East Anglia, 1991.

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LaMondia, James Arthur. Scantic, a new fusarium-wilt resistant broadleaf tobacco cultivar. New Haven: Connecticut Agricultural Experiment Station, 2001.

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Gullino, M. Lodovica, J. Katan, and Angelo Garibaldi. Fusarium wilts of greenhouse vegetable and ornamental crops. St. Paul, Minn., U.S.A: American Phytopathological Society, 2012.

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Book chapters on the topic "Fusarium wilt of banana"

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Ndayihanzamaso, Privat, Sheryl Bothma, Diane Mostert, George Mahuku, and Altus Viljoen. "An Optimised Greenhouse Protocol for Screening Banana Plants for Fusarium Wilt Resistance." In Efficient Screening Techniques to Identify Mutants with TR4 Resistance in Banana, 65–77. Berlin, Heidelberg: Springer Berlin Heidelberg, 2022. http://dx.doi.org/10.1007/978-3-662-64915-2_5.

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AbstractFusarium wilt, caused by the soil-borne fungus Fusarium oxysporum f. sp. cubense (Foc), is considered one of the most devastating diseases of banana in the world. Effective management of Fusarium wilt is only achieved by planting banana varieties resistant to Foc. Resistant bananas, however, require many years of breeding and field-testing under multiple geographical conditions. Field evaluation is reliable but time consuming and expensive. Small plant screening methods are, therefore, needed to speed up the evaluation of banana varieties for Foc resistance. To this end, a small plant screening method for resistance to banana Fusarium wilt is presented. The method proposes the planting of 2- to 3-month-old banana plants in soil amended with 10 g Foc-colonised millet seeds. Rhizome discoloration is then evaluated to rank the disease resistance response. The optimized millet seed technique could be useful in mass screening of newly developed genotypes for resistance to Foc.
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Usharani, T. R., H. D. Sowmya, C. Sunisha, and Sukhada Mohandas. "Engineering Resistance to Fusarium Wilt." In Banana: Genomics and Transgenic Approaches for Genetic Improvement, 211–26. Singapore: Springer Singapore, 2016. http://dx.doi.org/10.1007/978-981-10-1585-4_14.

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Wu, Yuanli, and Ganjun Yi. "Pre-Screening of Banana Genotypes for Fusarium Wilt Resistance by Using an In Vitro Bioassay." In Efficient Screening Techniques to Identify Mutants with TR4 Resistance in Banana, 33–45. Berlin, Heidelberg: Springer Berlin Heidelberg, 2022. http://dx.doi.org/10.1007/978-3-662-64915-2_3.

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AbstractIn the process of breeding and selection of banana for resistance to Fusarium wilt, it is important to conduct an efficient resistance screening test by artificial inoculation with Fusarium oxysporum f. sp. cubense (Foc) Tropical Race 4. So far, there are two types of early bioassays for screening Musa genotypes against Foc: a greenhouse and an in vitro bioassay. The most commonly used greenhouse bioassay is a pot-based system followed by a hydroponic system. Here we describe an in vitro bioassay characterized by in vitro inoculation of rooted banana plantlets grown on medium consisting of half-strength MS macronutrients and MS micronutrients. The disease response and evaluation results obtained through this in vitro bioassay correlates with that from a greenhouse screen and/or field evaluation. Given the importance of in vitro cell and tissue culture techniques for banana (mutation) breeding, promising resistant clones could be screened directly. This in vitro bioassay is a totally contained system compared with greenhouse methods and does not require an acclimatization step, thereby improving banana breeding efficiency. The in vitro pre-screening protocol and bioassay for Fusarium wilt resistance presented here is fast, space-effective, and accurate.
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Khiabani, Behnam Naserian. "In Vitro Based Mass-Screening Technique for Early Selection of Banana Mutants Resistant to Fusarium Wilt." In Efficient Screening Techniques to Identify Mutants with TR4 Resistance in Banana, 47–63. Berlin, Heidelberg: Springer Berlin Heidelberg, 2022. http://dx.doi.org/10.1007/978-3-662-64915-2_4.

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AbstractBanana and plantains are among the most valuable agricultural commodities in the world. Banana Fusarium wilt, caused by the soil-borne Fusarium oxysporum f. sp. cubense (Foc), is one of the most devastating diseases of banana globally. In the 1990s a new strain of Fusarium oxysporum called tropical race 4 (TR4) emerged in Southeast Asia that affected commercial Cavendish plantations. The development of resistant cultivars is an effective strategy for management of the disease. Field-based screening to identify Foc-resistant plants is time-consuming, expensive and is often challenged by variable environmental conditions. Here we present an early selection protocol enabling evaluation of the disease under in vitro conditions. This method provides a preliminary screening and allows evaluation of a large number of in vitro plantlets. Using this method, within a short time and in a small laboratory, breeders can evaluate thousands of banana plantlets, produced via irradiation. Subsequently, putative, disease-resistant mutant lines can be identified and evaluated in the field.
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Jankowicz-Cieslak, Joanna, Florian Goessnitzer, Sneha Datta, Altus Viljoen, Ivan Ingelbrecht, and Bradley J. Till. "Induced mutations for generating bananas resistant to Fusarium wilt tropical race 4." In Mutation breeding, genetic diversity and crop adaptation to climate change, 366–78. Wallingford: CABI, 2021. http://dx.doi.org/10.1079/9781789249095.0038.

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Abstract Bananas are a staple for more than 400 million people. Additionally, more than 16.5 million tonnes are exported, making it both an important food security and a cash crop. Productivity of Cavendish-type bananas is threatened by both abiotic and biotic stresses. The fact that triploid bananas are sterile, parthenocarpic and obligate vegetatively propagated makes them particularly susceptible to diseases, including Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc) tropical race 4 (Foc TR4). This is because continual clonal propagation has led to loss of genetic diversity. Additionally, lack of meiosis limits methods for breeding. Foc TR4 has been devastating Cavendish bananas in South-east Asia but has recently also been reported from Queensland in Australia, the Middle East and Mozambique, thus threatening global banana production. To address this, we are performing mutagenesis of in vitro propagated bananas to broaden the genetic diversity in order to find new alleles conferring disease resistance. We have developed methods for efficient induction of mutations in isolated apical meristems from shoot tips using chemical mutagens and ionizing radiation. Mutation discovery methods have been adapted to recover mutations including single point mutations and large deletions spanning millions of base pairs. We have created approximately 5000 mutated lines for forward-genetic screens to identify TR4 resistance in greenhouse- evaluated material. A subset of ca. 500 in vitro plantlets was subjected to glasshouse-based screening using a virulent F. oxysporum isolate. To date, 23 lines showing altered resistance responses to Foc TR4 have been identified.
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Jankowicz-Cieslak, Joanna, Ivan L. Ingelbrecht, and Bradley J. Till. "Mutation Detection in Gamma-Irradiated Banana Using Low Coverage Copy Number Variation." In Efficient Screening Techniques to Identify Mutants with TR4 Resistance in Banana, 113–27. Berlin, Heidelberg: Springer Berlin Heidelberg, 2022. http://dx.doi.org/10.1007/978-3-662-64915-2_8.

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AbstractMutagenesis of in vitro propagated bananas is an efficient method to introduce novel alleles and broaden genetic diversity. The FAO/IAEA Plant Breeding and Genetics Laboratory previously established efficient methods for mutation induction of in vitro shoot tips in banana using physical and chemical mutagens as well as methods for the efficient discovery of ethyl methanesulphonate (EMS) induced single nucleotide mutations in targeted genes. Officially released mutant banana varieties have been created using gamma rays, a mutagen that can produce large genomic changes such as insertions and deletions (InDels). Such dosage mutations may be particularly important for generating observable phenotypes in polyploids such as banana. Here, we describe a Next Generation Sequencing (NGS) approach in Cavendish (AAA) bananas to identify large genomic InDels. The method is based on low coverage whole genome sequencing (LC-WGS) using an Illumina short-read sequencing platform. We provide details for sonication-mediated library preparation and the installation and use of freely available computer software to identify copy number variation in Cavendish banana. Alternative DNA library construction procedures and bioinformatics tools are briefly described. Example data is provided for the mutant variety Novaria and cv Grande Naine (AAA), but the methodology can be equally applied for triploid bananas with mixed genomes (A and B) and is useful for the characterization of putative Fusarium Wilt TR4 resistant mutant lines described elsewhere in this protocol book.
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Noordin, Norazlina, Affrida Abu Hassan, Anis Nadia Mohd Faisol Mahadevan, Zaiton Ahmad, and Sakinah Ariffin. "Lab-Based Screening Using Hydroponic System for the Rapid Detection of Fusarium Wilt TR4 Tolerance/Resistance of Banana." In Efficient Screening Techniques to Identify Mutants with TR4 Resistance in Banana, 79–95. Berlin, Heidelberg: Springer Berlin Heidelberg, 2022. http://dx.doi.org/10.1007/978-3-662-64915-2_6.

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AbstractField-based screening and evaluation of banana plant tolerance or resistance to Fusarium oxysporum f. sp. cubense (Foc) Tropical Race 4 (TR4) or also known as Fusarium wilt TR4 is ideal though not always feasible. Alternatively, screening of banana plantlets at lab-stage seems to be an effective method for early detection of Foc TR4 tolerance. We present a simple hydroponic system, that allows plant to grow in a water-based condition. The system has two layers, the upper layer is a tray that has holes for plantlets to be placed where the root system is supported using an inert medium such as rock-wool. The lower layer is a perforated container filled with a water-based nutrient solution. For this lab-based screening, ex vitro gamma irradiated banana cv. Berangan (AAA) rooted plantlets with a pseudostem height of 10–15 cm were inoculated by soaking in a Foc TR4 conidial suspension (106 spores/ml) for 2 h under room temperature. The Foc TR4 inoculated rooted plantlets were screened using the hydroponic system and disease symptoms were scored. In this chapter, protocols on acclimatization of ex vitro irradiated rooted plantlets, inoculation with a Foc TR4 conidial suspension, lab- screening using hydroponic system, observation for early detection of disease symptoms and scoring of disease severity are presented.
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Hu, Chunhua, Yuanli Wu, and Ganjun Yi. "Gamma Irradiation of Embryogenic Cell Suspension Cultures from Cavendish Banana (Musa spp. AAA Group) and In Vitro Selection for Resistance to Fusarium Wilt." In Efficient Screening Techniques to Identify Mutants with TR4 Resistance in Banana, 21–30. Berlin, Heidelberg: Springer Berlin Heidelberg, 2022. http://dx.doi.org/10.1007/978-3-662-64915-2_2.

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AbstractIn this chapter, the establishment of embryogenic cell suspension (ECS) cultures using immature male flowers of triploid banana (Musa AAA Cavendish subgroup cv. ‘Brazil’), followed by somatic embryogenesis and plantlet regeneration is described. Mutation induction is achieved by exposing the ECS to gamma irradiation with the dose of 80 Gy. The mutagenized cell population is transferred to solid long-term suspension culture medium for 96 h to recover from mutagen treatment shock, followed by somatic embryo induction and development medium containing 20% crude culture filtrates from Fusarium oxysporum f. sp. cubense (Foc). After 90 days, the somatic embryos that survive are transferred to the germination medium containing 25% crude culture filtrates. The surviving mature somatic embryos are transferred to rooting medium after the fourth subculture on the germination medium containing 50% crude culture filtrates. Before transplanting in a Foc infected field, the in vitro plantlets are acclimatized and screened for resistance to Foc using a pot-based greenhouse bioassay.
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Domínguez, J., C. M. Rodríguez, and J. M. Hernández-Moreno. "Iron and Fusarium wilts in banana crops on Andic soils." In Iron Nutrition in Soils and Plants, 255–58. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-011-0503-3_37.

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Ploetz, Randy C. "Gone Bananas? Current and Future Impact of Fusarium Wilt on Production." In Plant Diseases and Food Security in the 21st Century, 21–32. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-57899-2_2.

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Conference papers on the topic "Fusarium wilt of banana"

1

Alekseeva, K. L., L. G. Smetanina, and A. V. Kornev. "Biological protection of tomato from Fusarium wilt." In PROCEEDINGS OF THE 3RD INTERNATIONAL CONFERENCE ON AUTOMOTIVE INNOVATION GREEN ENERGY VEHICLE: AIGEV 2018. Author(s), 2019. http://dx.doi.org/10.1063/1.5087309.

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"Transcriptional profiling of fusarium wilt in flax." In Bioinformatics of Genome Regulation and Structure/Systems Biology (BGRS/SB-2022) :. Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences, 2022. http://dx.doi.org/10.18699/sbb-2022-365.

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Zaitseva, L. A., N. A. Kudryavtsev, D. O. Morozov, and V. V. Chebanenko. "Agrotechnics, plant protection and immunity in improving the phytosanitary condition of crops Flax in Russia." In Растениеводство и луговодство. Тимирязевская сельскохозяйственная академия, 2020. http://dx.doi.org/10.26897/978-5-9675-1762-4-2020-148.

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The Federal scientific center for bast crops has long been creating varieties with high resistance to 2 diseases (rust and Fusarium wilt), and now successfilly solves the problem of forming resistance to 3 (rust, Fusarium and Anthracnose) and even to 4 diseases (rust, Fusarium, Anthracnose and Pasmo). New proposals for phytosanitary monitoring and forecasting racionalize plant protection in relation to flax production. Ecologized biological preparations (for example, Vitaplan, Sternifag) are effective against flax diseases (Bacteriosis, Anthracnose, Mottling, etc.) and contribute to the preservation of the flax crop.
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Chen, Bo-Han, Yen-Chieh Ouyang, Mang Ou-Yang, Horng-Yuh Guo, Tsang-Sen Liu, Hsian-Min Chen, Chao-Cheng Wu, Chia-Hsien Wen, Chgein-I. Chang, and Min-Shao Shih. "Fusarium Wilt Inspection for Phalaenopsis Using Uniform Interval Hyperspectral Band Selection Techniques." In IGARSS 2020 - 2020 IEEE International Geoscience and Remote Sensing Symposium. IEEE, 2020. http://dx.doi.org/10.1109/igarss39084.2020.9324496.

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Sadikova, Surayyo. "BIOLOGICAL EFFICACY OF FUNGICIDES AGAINST FUSARIUM WILT IN FIELD CONDITIONS OF HOT PEPPER." In THEORETICAL AND EMPIRICAL SCIENTIFIC RESEARCH: CONCEPT AND TRENDS. European Scientific Platform, 2020. http://dx.doi.org/10.36074/24.07.2020.v1.37.

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Гринько, Н. Н. "Collection of plant genetic resources Cucumis sativus VIR - source resistance to Fusarium wilt." In НАУКА РОССИИ: ЦЕЛИ И ЗАДАЧИ. НИЦ «Л-Журнал», 2018. http://dx.doi.org/10.18411/sr-10-04-2018-59.

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Lee, Meng-Chueh, Kenneth-Yeonkone Ma, Yen-Chieh Ouvang, Mang Ou-Yang, Horng-Yuh Guo, Tsang-Sen Liu, Hsian-Min Chen, Chao-Cheng Wu, and Chgein-I. Chang. "Detection of Fusarium Wilt on Phalaenopsis Stem Base Region Using Band Selection Techniques." In IGARSS 2018 - 2018 IEEE International Geoscience and Remote Sensing Symposium. IEEE, 2018. http://dx.doi.org/10.1109/igarss.2018.8517781.

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Jeon, YA, JH Rhee, JS Sung, HJ Baek, OS Hur, and NY Rho. "Radish germplasm with fusarium wilt-resistance as material for breeding disease-resistant varieties." In GA 2017 – Book of Abstracts. Georg Thieme Verlag KG, 2017. http://dx.doi.org/10.1055/s-0037-1608350.

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Yang, Zhengang, Feiqi Deng, and Weizhang Liu. "Intelligent Forecast for Cucumber Fusarium Wilt Combining Case-based Reasoning With Self-organizing Maps." In Third International Conference on Natural Computation (ICNC 2007) Vol V. IEEE, 2007. http://dx.doi.org/10.1109/icnc.2007.449.

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Savchuk, N. V., E. G. Yurchenko, S. V. Vinogradova, and E. V. Porotikova. "Causative agents of Fusarium wilt of the reproductive organs of grapes. Ways of infection." In CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-2020-5-9-10-45.

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The possibility of grapevines infecting with F. proliferatum and F. oxysporum at the flowering stage is proven. The methods of infection penetration can be different, both through injuring the conductive tissue of inflorescence (axes, ridges), and through flowers (in case of violation of the integrity of delicate tissues by the wind, agricultural tools, etc.). However, the damage caused to the plant by the disease in all these cases is almost the same.
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Reports on the topic "Fusarium wilt of banana"

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Kistler, Harold Corby, and Talma Katan. Identification of DNA Unique to the Tomato Fusarium Wilt and Crown Rot Pathogens. United States Department of Agriculture, September 1995. http://dx.doi.org/10.32747/1995.7571359.bard.

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Wilt and crown rot are two important diseases of tomato caused by different strains ("formae speciales") of the fungus, Fusarium oxysporum. While both pathogens are members of the same fungal species, each differs genetically and resistance to the diseases is controlled by different genes in the plant. Additionally, the formae speciales differ in their ecology (e.g. optimal temperature of disease development) and epidemiology. Nevertheless, the distinction between these diseases based on symptoms alone may be unclear due to overlapping symptomatology. We have found in our research that the ambiguity of the pathogens is further confounded because strains causing tomato wilt or crown rot each may belong to several genetically and phylogenetically distinct lineages of F. oxysporum. Furthermore, individual lineages of the pathogen causing wilt or crown rot may themselves be very closely related. The diseases share the characteristic that the pathogen's inoculum may be aerially dispersed. This work has revealed a complex evolutionary relationship among lineages of the pathogens that makes development of molecular diagnostic methods more difficult than originally anticipated. However, the degree of diversity found in these soil-borne pathogens has allowed study of their population genetics and patterns of dispersal in agricultural settings.
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Kistler, Harold Corby, Talma Katan, and Dani Zamir. Molecular Karyotypes of Pathogeic Strains of Fusarium oxysporum. United States Department of Agriculture, June 1995. http://dx.doi.org/10.32747/1995.7604927.bard.

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Genetic diversity of pathogenic strains of the fungus Fusarium oxysporum was determied by analysis of electrophoretic karyotype, as well as by DNA variation detected by Restriction Fragment Length Polymorphisms (RFLPs) and Random Amplified Polymorphic DNAs (RAPDs). The electrophoretic karyotypes for 130 isolates of the fungus pathogenic to tomato, melon, and banana were analyzed. Electrophoretic karyotype variation, reflected in differences in apparent chromosome number and genome size, was observed even among isolates from the same host and sub specific category. Sub specific categories studied were forma specialis, vegetative compatibility group (VCG) and race. Chromosome number and genome size variation was less for isolates within the same VCG than for the collection of isolates as a whole. RFLP and RAPD analysis were performed on 62 isolates of F. oxysporum from tomato and melon. Polygenetic trees were constructed from genetic diversity data. The results support the hypothesis that isolates belonging to the same VCG originate from a single ancestor compared to other isolates. The results do not support the hypothesis that all isolates belonging to the same forma specialis originate from a common ancestor. These conclusions have profound implication for breeding resistance to diseases caused by particular formae speciales of F. oxysporum.
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Strategic assessment of banana fusarium wilt research priorities. International Potato Center, 2017. http://dx.doi.org/10.4160/23096586rtbwp20182.

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