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Relevant bibliographies by topics / Fusobacterium nucleatum

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Academic literature on the topic 'Fusobacterium nucleatum'

Author: Grafiati

Published: 4 June 2021

Last updated: 15 June 2025

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Journal articles on the topic "Fusobacterium nucleatum"

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Ganesan, Kumar, Songhe Guo, Sundaz Fayyaz, Ge Zhang, and Baojun Xu. "Targeting Programmed Fusobacterium nucleatum Fap2 for Colorectal Cancer Therapy." Cancers 11, no. 10 (2019): 1592. http://dx.doi.org/10.3390/cancers11101592.

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Colorectal patients generally have the maximum counts of Fusobacterium nucleatum (F. nucleatum) in tumors and elevate colorectal adenomas and carcinomas, which show the lowest rate of human survival. Hence, F. nucleatum is a diagnostic marker of colorectal cancer (CRC). Studies demonstrated that targeting fusobacterial Fap2 or polysaccharide of the host epithelium may decrease fusobacteria count in the CRC. Attenuated F. nucleatum-Fap2 prevents transmembrane signals and inhibits tumorigenesis inducing mechanisms. Hence, in this review, we hypothesized that application of genetically programmed fusobacterium can be skillful and thus reduce fusobacterium in the CRC. Genetically programmed F. nucleatum is a promising antitumor strategy.

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Han, Yiping W., Akihiko Ikegami, Chythanya Rajanna, et al. "Identification and Characterization of a Novel Adhesin Unique to Oral Fusobacteria." Journal of Bacteriology 187, no. 15 (2005): 5330–40. http://dx.doi.org/10.1128/jb.187.15.5330-5340.2005.

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ABSTRACT Fusobacterium nucleatum is a gram-negative anaerobe that is prevalent in periodontal disease and infections of different parts of the body. The organism has remarkable adherence properties, binding to partners ranging from eukaryotic and prokaryotic cells to extracellular macromolecules. Understanding its adherence is important for understanding the pathogenesis of F. nucleatum. In this study, a novel adhesin, FadA (Fusobacterium adhesin A), was demonstrated to bind to the surface proteins of the oral mucosal KB cells. FadA is composed of 129 amino acid (aa) residues, including an 18-aa signal peptide, with calculated molecular masses of 13.6 kDa for the intact form and 12.6 kDa for the secreted form. It is highly conserved among F. nucleatum, Fusobacterium periodonticum, and Fusobacterium simiae, the three most closely related oral species, but is absent in the nonoral species, including Fusobacterium gonidiaformans, Fusobacterium mortiferum, Fusobacterium naviforme, Fusobacterium russii, and Fusobacterium ulcerans. In addition to FadA, F. nucleatum ATCC 25586 and ATCC 49256 also encode two paralogues, FN1529 and FNV2159, each sharing 31% identity with FadA. A double-crossover fadA deletion mutant, F. nucleatum 12230-US1, was constructed by utilizing a novel sonoporation procedure. The mutant had a slightly slower growth rate, yet its binding to KB and Chinese hamster ovarian cells was reduced by 70 to 80% compared to that of the wild type, indicating that FadA plays an important role in fusobacterial colonization in the host. Furthermore, due to its uniqueness to oral Fusobacterium species, fadA may be used as a marker to detect orally related fusobacteria. F. nucleatum isolated from other parts of the body may originate from the oral cavity.

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Allen-Vercoe, Emma, Jaclyn Strauss, and Kris Chadee. "Fusobacterium nucleatum." Gut Microbes 2, no. 5 (2011): 294–98. http://dx.doi.org/10.4161/gmic.2.5.18603.

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Bashir, Arif, Abid Y. Miskeen, Ashaqullah Bhat, Khalid M. Fazili, and Bashir A. Ganai. "Fusobacterium nucleatum." European Journal of Cancer Prevention 24, no. 5 (2015): 373–85. http://dx.doi.org/10.1097/cej.0000000000000116.

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Yocum, Denise. "Fusobacterium nucleatum." Journal of the American Academy of Physician Assistants 29, no. 12 (2016): 1–4. http://dx.doi.org/10.1097/01.jaa.0000508216.58368.74.

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Dussmann, Heiko, Barry Maguire, Caoimbhe Burke, et al. "Abstract 6531: High resolution analysis of Fusobacterium infection in colorectal cancer." Cancer Research 85, no. 8_Supplement_1 (2025): 6531. https://doi.org/10.1158/1538-7445.am2025-6531.

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Previous research provided evidence pointing towards an important role for bacterial infection with Fusobacterium nucleatum (F. nucleatum) and F. animalis (previuosly F. nucleatum subsp. animalis) in colorectal cancer (CRC) progression. Both are oral commensal Gram negative anaerobes with recent evidence suggesting F. animalis predominates in CRC (Zepeda-Rivera et al., 2024). We recently applied spatial transcriptomics to demonstrate local effects of Fusobacterium infection on cancer and immune cell gene expression in CRC (Duggan et al, Gut Microbes, 2024). While Fusobacterium infection was observed in specific patches in the tumors, the precise cell types infected with bacteria and the precise localization of bacteria in cells still requires further elucidation. Commercially available strains of F. nucleatum (ATCC 25586) and F. animalis (ATCC 51191) were purchased and separately co-cultured with MDA-MB-468 breast cancer cells. Immuno cytochemistry using a commercial Fusobacteria antibody (ANT0084) and a Fusobacteria antiserum (Prof Slade group), along with pancytokeratin and Hoechst staining was undertaken. High resolution confocal microscopy using an LSM 980 Airyscan 2 microscope (Carl Zeiss, Jena, Germany) was performed to create image stacks of optical sections. CRC tissue sections were subsequently prepared and stained by immunohistochemistry (IHC) for pancytokeratin, CD68, and Fusobacteria (Slade antisera). Imaging of the full tumor face was undertaken at a lower resolution using Cell DIVE (Leica Microsystems, Wetzlar, Germany) to select regions rich in Fusobacteria for further study. Selected regions were then imaged using high resolution confocal microscopy. Fusobacteria antisera stained both F. nucleatum and F. animalis while the commercially produced antibody stained only F. nucleatum. Both bacteria can populate the cell surroundings, cell surface, cytoplasm as well as nucleus. The typical morphology of both F. nucleatum and F. animalis is seen, allowing antibody validation. In CRC tissue intracellular bacteria are seen in both pancytokeratin positive tumor epithelial cells as well as in CD68 (Cluster of Differentiation 68) positive cells (tumor associated macrophages). Tumor associated macrophages (TAMs) have an important role in the tumor immmune microenvironment. In particular, studies have highlighted the importance of M1/M2 macrophage polarization on immune-evasion and prognosis. Our spatial transcriptomic study highlighted reduced M2 polarization as associated with increased Fusobacterial load. The intracellular location of Fusobacteria may suggest a mechanism for altered macrophage polarization. References: Zepeda-Rivera et al, Nature Vol 628, pages 424-432 (2024)Flanagan et al, Eur J Clin Microbiol Infect Dis. 2014 Aug;33(8):1381-90 Duggan et al, Gut Microbes. 2024 Jan-Dec;16(1):2350149. Citation Format: Heiko Dussmann, Barry Maguire, Caoimbhe Burke, Arman Raman, John Burke, William M. Gallagher, Jochen H. Prehn. High resolution analysis of Fusobacterium infection in colorectal cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2025; Part 1 (Regular Abstracts); 2025 Apr 25-30; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2025;85(8_Suppl_1):Abstract nr 6531.

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Mandal, Devi Prasad, Neeta Mohanty, Paresh Kumar Behera, et al. "A Plausible Proposition of CCL20-Related Mechanism in Fusobacterium nucleatum-Associated Oral Carcinogenesis." Life 11, no. 11 (2021): 1218. http://dx.doi.org/10.3390/life11111218.

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Objective: The objective of this prospective observational case–control study is to evaluate the prevalence of Fusobacterium nucleatum in the tissues of oral squamous cell carcinoma (OSCC). Reconnoitering the CCL20-related mechanism of carcinogenesis in Fusobacterium nucleatum-positive OSCC is another objective. Methodology: Tissues from 50 OSCC patients and 30 healthy oral tissues were collected. The prevalence of Fusobacterium nucleatum was evaluated in both tumour and healthy tissue by polymerase chain reaction. The immunohistochemistry of OSCC tissues was conducted to evaluate the difference in the expression of CCL20 between Fusobacterium nucleatum-positive and -negative OSCC tissues. Results: Fusobacterium nucleatum was significantly (p < 0.001) prevalent in OSCC tissues (74%), compared to healthy tissues (26%). No association of Fusobacterium nucleatum or CCL20 immuno-expression with any clinical or histopathological features of OSCC was observed. While the intensity of CCL20 immuno-expression did not differ (p = 0.053), the CCL20-positive cell population was significantly different (p = 0.034) between Fusobacterium nucleatum-positive and -negative OSCC. Conclusion: Fusobacterium nucleatum is possibly prevalent in oral cancer tissues in the Indian population. By using immunohistochemistry, this is the first study to propose that the carcinogenesis in Fusobacterium nucleatum-positive OSCC may be CCL20-related. The findings enrich the knowledge of mechanisms involved in Fusobacterium nucleatum-mediated oral carcinogenesis.

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Beavers, Bruce R. "FUSOBACTERIUM NUCLEATUM PYOMYOSITIS." Orthopedics 15, no. 2 (1992): 208–11. http://dx.doi.org/10.3928/0147-7447-19920201-17.

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Bachrach, Gilad, Susan Kinder Haake, Alon Glick, et al. "Characterization of the Novel Fusobacterium nucleatum Plasmid pKH9 and Evidence of an Addiction System." Applied and Environmental Microbiology 70, no. 12 (2004): 6957–62. http://dx.doi.org/10.1128/aem.70.12.6957-6962.2004.

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ABSTRACT Fusobacterium nucleatum is an important oral anaerobic pathogen involved in periodontal and systemic infections. Studies of the molecular mechanisms involved in fusobacterial virulence and adhesion have been limited by lack of systems for efficient genetic manipulation. Plasmids were isolated from eight strains of F. nucleatum. The smallest plasmid, pKH9 (4,975 bp), was characterized and used to create new vectors for fusobacterial genetic manipulation. DNA sequence analysis of pKH9 revealed an open reading frame (ORF) encoding a putative autonomous rolling circle replication protein (Rep), an ORF predicted to encode a protein homologous to members of the FtsK/SpoIIIE cell division-DNA segregation protein family, and an operon encoding a putative toxin-antitoxin plasmid addiction system (txf-axf). Deletion analysis localized the pKH9 replication region in a 0.96-kbp fragment. The pKH9 rep gene is not present in this fragment, suggesting that pKH9 can replicate in fusobacteria independently of the Rep protein. A pKH9-based, compact Escherichia coli-F. nucleatum shuttle plasmid was constructed and found to be compatible with a previously described pFN1-based fusobacterial shuttle plasmid. Deletion of the pKH9 putative addiction system (txf-axf) reduced plasmid stability in fusobacteria, indicating its addiction properties and suggesting it to be the first plasmid addiction system described for fusobacteria. pKH9, its genetic elements, and its shuttle plasmid derivatives can serve as useful tools for investigating fusobacterial properties important in biofilm ecology and pathogenesis.

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Shhadeh, Amjad, Liat Dassa, Jamal Fahoum, et al. "Abstract 5893: Suppression of anti-tumor immunity by the Fusobacterium nucleatum protease fusolisin." Cancer Research 83, no. 7_Supplement (2023): 5893. http://dx.doi.org/10.1158/1538-7445.am2023-5893.

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Abstract Fusobacterium nucleatum is an oral pathogen associated with periodontal disease, preterm births and the exacerbation of colorectal, esophageal, pancreatic, and breast cancers. F. nucleatum was previously found to inhibit killing of cancer cells by natural killer (NK) cells and tumor infiltrating T cells by inducing the NK cells killing-suppressing receptors TIGIT and CEACAM1. In this study, we show that the incubation of F. nucleatum with primary human natural killer (NK) cells causes the cleavage of the activating receptors CD16, NKp44 and NKp46 by fusolisin. Fusolisin is a fusobacterial outer-membrane, auto-transporter, serine protease and to date the only functional protease found in F. nucleatum. We previously showed that fusolisin is essential for fusobacterial growth in culture. High expression of a functional recombinant fusolisin in E. coli was enabled using codon optimization and replacement of the fusolisin's signal peptide with that of the E. coli OmpA. Recombinant fusolisin was found to cleave the same NK-activating receptors degraded by F. nucleatum. Cleavage of these activating receptors by fusolisin inhibited NK cells activity including killing of tumor cells in-vitro and in-vivo. Our results provide a new bacterial protease-dependent mechanism in which tumors colonized by F. nucleatum are protected from NK cells attack by exploiting fusolisin proteolytic activity. Importantly, our previous and current results suggest that fusolisin might serve to target for tumor-colonized fusobacteria. Citation Format: Amjad Shhadeh, Liat Dassa, Jamal Fahoum, Nicole Haj, Reuven Wiener, Ofer Mandelboim, Gilad Bachrach. Suppression of anti-tumor immunity by the Fusobacterium nucleatum protease fusolisin [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 5893.

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Dissertations / Theses on the topic "Fusobacterium nucleatum"

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Lee, Felix S. "Amino acid catabolism in Fusobacterium nucleatum and Fusobacterium varium." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0017/MQ57203.pdf.

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Chen, Jianhe. "Some physiological studies of Fusobacterium nucleatum /." Title page, summary and contents only, 1994. http://web4.library.adelaide.edu.au/theses/09DSM/09dsmc518.pdf.

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Gravel, Chantal. "Effets de paramètres environnementaux sur Fusobacterium nucleatum." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/MQ38102.pdf.

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Vasstrand, Endre N. "The rigid cell wall layer of Fusobacterium nucleatum Fev1 an enzymatic and chemical analysis /." Bergen : A/S West-Print, 1986. http://catalog.hathitrust.org/api/volumes/oclc/48051225.html.

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Suchett-Kaye, George. "Analyse clonale de fusobacterium nucleatum chez des sujets sains." Lyon 1, 1999. http://www.theses.fr/1999LYO1T183.

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Ogawa, Alice Tayoko. "Caracterização das atividades proteolíticas da bactéria anaeróbia fusobacterium nucleatum." Universidade Estadual de Londrina. Centro de Ciências Biológicas. Programa de Pós-Graduação em Microbiologia, 2004. http://www.bibliotecadigital.uel.br/document/?code=vtls000110059.

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Foram determinadas as atividades proteolíticas do bacilo anaeróbio Fusobacterium nucleatum, presente em várias infecções oral e sistêmica humana. As reações foram otimizadas quanto a pH, temperatura, tempo de reação, volume da fonte de enzima e concentração de substrato. Foram utilizados os substratos sintéticos b-naftilamida (Cys-Na, Ser-Na, Leu-Na, Glu-Na, Lys-Na e BANA), o carboxi-L-tirosina p-nitrofeniléster (CTN) e o substrato natural azoalbumina. Reação do sobrenadante ocorreu apenas com o substrato Glu-Na. Nas células vivas, as reações ocorreram com Cys-Na, Ser-Na e Glu-Na; e com as células lisadas, com os substratos Glu-Na, Leu-Na e CTN. O pH ótimo variou de 6,0 a 7,5 em todas as reações, com excessão para o CTN (pH 13). A temperatura ótima oscilou entre 30 e 40°C. O tempo ótimo de reação foi 60 minutos, exceto para as células vivas com o Glu-Na (40 min), células lisadas (20 min) e o substrato CTN (80 min). Não houve atividade com a azoalbumina. A atividade enzimática foi avaliada por vários inibidores de protease, sendo detectada a presença de metalo, serina, cisteína e aspartato proteases.<br>The proteolytic activities of the anaerobe bacillus Fusobacterium nucleatum present in several oral and systemic human infections were determined. The reactions were optimized in their pH, temperature, reaction time, enzyme source and substrate volume. The synthetic substrates b-?naphthylamides (Cys-Na, Ser-Na, Leu-Na, Glu-Na, Lys-Na and BANA), carboxi-L-tyrosine p-nitrophenylester (CTN), and natural substrate azoalbumin were used. With supernatants reaction occurred only with Glu-Na. In the living cells, reacted with Cys-Na, Ser-Na and Glu-Na; and lysate cells with Glu-Na, Leu-Na and CTN. Optimal pH ranged from 6.0 to 7.5 in all the reaction, except for CTN (pH 13). Optimal temperature oscillated between 30 and 40ºC. The optimal reaction time was 60 minutes, except for Glu-Na with living cells (40 min), lysate cells (20 min), and CTN substrate (80 min). There was no activity with azoalbumin. The enzyme activity by several protease inhibitors was assessed and the presence of metallo, serine, cysteine and aspartato protease was detected.

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Gunadi, Achmad. "Studies on a peptidase from Fusobacterium nucleatum ATCC 25586 /." Title page, contents and summary only, 1997. http://web4.library.adelaide.edu.au/theses/09PH/09phg975.pdf.

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Kiewiet, Paola Thérèse. "The production and characterisation of monoclonal antibodies to fusobacterium nucleatum." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1992. http://hub.hku.hk/bib/B3121082X.

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Kiewiet, Paola Thérèse. "The production and characterisation of monoclonal antibodies to fusobacterium nucleatum /." Hong Kong : University of Hong Kong, 1992. http://sunzi.lib.hku.hk/hkuto/record.jsp?B13290095.

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Midena, Raquel Zanin. "Suscetibilidade antimicrobiana e protocolo de purificação de RNA para análise de expressão gênica de isolados clínicos de Fusobacterium nucleatum." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/25/25147/tde-26022016-143429/.

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Fusobacterium nucleatum é uma espécie bacteriana Gram-negativa, anaeróbia estrita e uma das espécies frequentemente encontradas nas infecções primárias do sistema de canais radiculares. Esta espécie tem grande importância na formação de biofilmes por ser uma ponte de união entre espécies que não são capazes de interagir. Os micro-organismos constituintes de biofilmes trocam material genético, aumentando a tolerancia dos mesmos e é quase impossível um isolado clínico ter os genes totalmente iguais à cepa padrão de coleções de cultura como da ATCC (American Type Culture Colection). O presente estudo investigou a espécie bacteriana anaeróbia Fusobacterium nucleatum isolada de canais radiculares, comparando-a com sua cepa de referência. Foi feito a comparação da suscetibilidade microbiana in vitro por meio de cultura microbiológica pelo método do E-test, com as cepas em crescimento planctônico e em biofilme. Também foi definido um protocolo de Purificação de RNA para esta espécie em ambas as condições de crescimento. As cepas clínicas de F. nucelatum foram isoladas por meio da cultura microbiológica de 23 pacientes que apresentavam dentes com infecção primária e periodontite apical visível em radiografia. Foi feito isolamento e identificação da espécie por série bioquímica com testes comerciais (Sistema Api, Bio-Meriéux, França) e PCR convencional, sendo no total 4 isolados clínicos investigados. Foi verificada a suscetibilidade antimicrobiana dos seguintes antibióticos: Amoxicilina, Amoxicilina + ácido clavulânico, Ampicilina, Azitromicina, Clindamicina, Eritromicina e Metronidazol. O protocolo para purificação de RNA foi feito com microesferas de zircônia, dispositivo bead beater, kit comercial RNeasy (Qiagen) e transcrição para DNA complementar (cDNA). A qualidade da purificação foi testada quanto a sua capacidade de amplificação pela reação em cadeia da polimerase em tempo real (qPCR) utilizando primer para o gene 16s RNA específico para F. nucelatum. Todas as cepas testadas foram 100% suscetíveis a Amoxicilina + ácido clavulânico, Ampicilina, Azitromicina, Clindamicina e Metronidazol. Ambos os tipos de crescimento bacteriano demonstraram resistência somente à eritromicina. O protocolo proposto para a purificação do RNA de F. nucelatum dos isolados em crescimento planctônico e em biofilme foi eficaz. A média do rendimento do RNA das amostras para as bactérias em crescimento planctônico foi de 514,2 ng/μL (DP ± 397,7) e para as amostras em biofilme foi de 377,1 ng/μL (DP± 144,1). Os valores encontrados sugerem uma boa qualidade de RNA, livre de contaminação por proteínas. Todas as cepas de Fusobacterium nucleatum isoladas de canais radiculares, assim como a cepa ATCC foram suscetíveis aos antibióticos testados, com exceção do antibiótico eritromicina em ambos os tipos de crescimento bacteriano. As bactérias em biofilme apresentaram aumento na tolerância frente aos agentes antimicrobianos, com diferença estatística. O protocolo estabelecido para a purificação do RNA de cepas de Fusobacterium nucleatum cultivadas em fase planctônica e em biofilmes teve êxito com amplificação por qPCR.<br>Fusobacterium nucleatum is a Gram-negative bacterial species, strict anaerobic and one of the species often found in primary infection of the root canal system. This species has great importance in biofilm formation to be a union bridge between species which are not able to act alone. The constituent microorganisms of the biofilm exchange each tother genetic material, increasing the strength of them. It is almost impossible for a clinical isolate have genes totally equal to a standard strain, such as strains of culture collections like ATCC (American Type Culture Collection). The present study investigated anaerobic bacterial species Fusobacterium nucleatum isolated from root canals, comparing them to the ATCC strain. The microbial in vitro susceptibility of biofilm and planktonic growth of the strains was compared by means of microbiological culture and the E-test method, with the antibiotics Amoxicillin, Amoxicillin + clavulanic acid, Ampicillin, Azithromycin, Clindamycin, Eritromycin and Metronidazole.. Also, a RNA Purification protocol for the strains under the same growth conditions was defined. Clinical isolates were obtained by microbiological samples of patients with teeth with pulp necrosis and apical periodontitis visible on radiographs. The species isolation and identification were performed using commercial biochemical tests (Sistema Api, Bio-Meriéux, France) and conventional PCR, obtaining four clinical isolates. The protocol for RNA purification was done with zirconia beads, bead beater device and commercial kit RNeasy (Qiagen) and transcribed into complementary DNA (cDNA). The quality of purification was tested for its ability of amplification by real time polymerase chain reaction (qPCR) using primer for the gene 16s RNA specific for F. nucleatum. All tested trains were 100% susceptible to amoxicillin + clavulanic acid, ampicillin, azithromycin, clindamycin and metronidazole. Both types of bacterial growth showed resistance to Erythromycin. Bacteria in biofilm showed a decrease in susceptibility to all antibiotics, but without statistical difference. The protocol proposed for the purification of RNA of F. nucleatum was effective, in the planktonic and the biofilm growth. The average yield of RNA samples for bacteria in planktonic growth was 514.2 ng /μL (SD ± 397.7) and the samples in biofilm was 377.1 ng / μL (SD ± 144.1). These found values suggest a good quality of RNA, free of protein contamination. The established protocol for the purification of the RNA of the Fusobacterium nucleatum strains, grown in biofilm and planktonic phase, had successfully amplified by qPCR.

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Books on the topic "Fusobacterium nucleatum"

1

So, Jeewon. Inflammatory Pathways and Prevention Therapies in Placental Infection by Fusobacterium nucleatum. [publisher not identified], 2019.

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Book chapters on the topic "Fusobacterium nucleatum"

1

Yoshida, Akihiro, and Akihiko Ikegami. "Genetic Transformation of Fusobacterium nucleatum." In Periodontal Pathogens. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0939-2_5.

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Kabwe, Mwila, Teagan Brown, Heng Ku, Stuart Dashper, and Joseph Tucci. "Isolation and Functional Characterization of Fusobacterium nucleatum Bacteriophage." In Methods in Molecular Biology. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1518-8_4.

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To, Kevin, Timmie Britton, and Hung Ton-That. "Visualization of a Cell Wall Hydrolase Inhibitor in Fusobacterium nucleatum by Immunofluorescence Microscopy." In Methods in Molecular Biology. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3491-2_3.

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Bibek, G. C., Peng Zhou, and Chenggang Wu. "A New Method for Gene Deletion to Investigate Cell Wall Biogenesis in Fusobacterium nucleatum." In Methods in Molecular Biology. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3491-2_6.

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Walker, W. Allan. "Colorectal cancer and the microbiome: dysplasia, probiotics, and Fusobacterium nucleatum." In Colorectal Neoplasia and the Colorectal Microbiome. Elsevier, 2020. http://dx.doi.org/10.1016/b978-0-12-819672-4.00005-2.

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Bhonchal Bhardwaj, Sonia, and Seema Kumari. "Oral Bacteriophages." In Bacteriophages in Therapeutics. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.100269.

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Bacteriophage or phage therapy involves using phages or their products as bio-agents for the treatment or prophylaxis of bacterial infections or diseases. Bacteriophages have the ability to regulate the oral microflora by lysing sensitive bacterial cells and releasing bacterial components with pro-inflammatory activity. Bacteriophages carry specific polysaccharide depolymerases that aid viral penetration and can disrupt the pathogenic process associated with biofilm and exopolysaccharide in the oral cavity. Oral diseases are mainly caused by biofilm forming microorganisms and phages are now being used for biocontrol of oral biofilms. Phages for Actinomyces species, Aggregatibacter actinomycetemcomitans, Enterococcus faecalis, Fusobacterium nucleatum, Lactobacillus species, Neisseria species, Streptococcus species, and Veillonella species have been isolated and characterized. Bacteriophages could be considered as potential therapeutic tools for the elimination of caries, periodontitis, and other diseases of the oral cavity.

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Gaba, Fariah I., Raquel Carcelen Gonzalez, and Raquel Gonzalez Martinez. "The Significance of Oral Fusobacterium nucleatum in Female Breast Cancer: A Brief Overview." In Research Developments in Medicine and Medical Science Vol. 4. B P International (a part of SCIENCEDOMAIN International), 2023. http://dx.doi.org/10.9734/bpi/rdmms/v4/5722a.

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Taibi, Amel, Zoe Lofft, Julia MW Wong, et al. "Diet—microbiome interaction in colorectal cancer: a potentially discriminatory role for Fusobacterium nucleatum." In Colorectal Neoplasia and the Colorectal Microbiome. Elsevier, 2020. http://dx.doi.org/10.1016/b978-0-12-819672-4.00009-x.

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Poole Sophie, Singhrao Sim K., Chukkapalli Sasanka, et al. "Active Invasion of Porphyromonas gingivalis and Infection-Induced Complement Activation in ApoE." In Advances in Alzheimer’s Disease. IOS Press, 2017. https://doi.org/10.3233/978-1-61499-706-1-119.

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Periodontal disease is a polymicrobial inflammatory disease that leads to chronic systemic inflammation and direct infiltration of bacteria/bacterial components, which may contribute to the development of Alzheimer's disease. ApoE&minus;/&minus;mice were orally infected (n=12) with Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, and Fusobacterium nucleatum as mono-and polymicrobial infections. ApoE&minus;/&minus;mice were sacrificed following 12 and 24 weeks of chronic infection. Bacterial genomic DNA was isolated from all brain tissues except for the F. nucleatum mono-infected group. Polymerase chain reaction was performed using universal 16s rDNA primers and species-specific primer sets for each organism to determine whether the infecting pathogens accessed the brain. Sequencing amplification products confirmed the invasion of bacteria into the brain during infection. The innate immune responses were detected using antibodies against complement activation products of C3 convertase stage and the membrane attack complex. Molecular methods demonstrated that 6 out of 12 ApoE&minus;/&minus;mice brains contained P. gingivalis genomic DNA at 12 weeks (p=0.006), and 9 out of 12 at 24 weeks of infection (p=0.0001). Microglia in both infected and control groups demonstrated strong intracellular labeling with C3 and C9, due to on-going biosynthesis. The pyramidal neurons of the hippocampus in 4 out of 12 infected mice brains demonstrated characteristic opsonization with C3 activation fragments (p=0.032). These results show that the oral pathogen P. gingivalis was able to access the ApoE&minus;/&minus;mice brain and thereby contributed to complement activation with bystander neuronal injury.

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Vaz, Adriana M., Emanuelle L, and Tatiana F. "PERFIL CLÍNICO E MICROBIOLÓGICO DAS DOENÇAS PERI-IMPLANTARES ANTES E APÓS TERAPIAS MECÂNICAS ANTI-INFECCIOSAS." In Ciências da Saúde: desafios e potencialidades em pesquisa - Volume 2. Editora Científica Digital, 2022. http://dx.doi.org/10.37885/230212018.

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O objetivo deste estudo foi avaliar os efeitos clínicos e microbiológicos de terapias anti-infecciosas mecânicas para mucosite e peri-implantite. Material e métodos: Indivíduos com pelo menos um implante dentário foram designados para SAÚDE (N=10), mucosite (N=12) ou peri-implantite (n=13) grupos. Implantes com mucosite ou peri- implantite foram descontaminados por meio de curetas de teflon e pó abrasivo de carbonato de sódio, realizada por retalho aberto para peri-implantite e sem cirurgia para mucosite. Placa visível (IPV), sangramento marginal (SM), sangramento à sondagem (SS), supuração (SUP), profundidade de sondagem (PS) e nível de inserção clínico relativo (NICr) foram avaliados no início e 3 meses após as terapias. Ao mesmo tempo, amostras de biofilme subgengival foram coletadas de cada implante e analisadas por meio da técnica de hibridização Checkerboard DNA-DNA para 40 espécies bacterianas. Resultados: Todos os parâmetros clínicos melhoraram 3 meses após a terapia nos grupos de mucosite e peri-implantite (P0,05). A redução média em NIC foi de 1,4 - 1,2 mm e 2,3 - 1,6 mm, e foi de 1,3 - 1,2 mm e 3,1 - 1,7 mm em SS para mucosite e peri-implantite, respectivamente. Níveis de Treponema denticola, Tanerella forsythia e Parvimonas micra, e de Fusobacterium nucleatum ss nucleatum,foram significativamente reduzidos após a terapia de peri-implantite e após a terapia de mucosite, respectivamente (Po0,05). Além disso, contagens de Porphyromons gingivalis, Treponema socranskiie as proporções de complexo vermelho foram reduzidas em ambos os grupos 3 meses após os tratamentos (P0,05). Conclusão: As terapias mecânicas sozinhas foram eficazes no tratamento de mucosite e peri-implantite durante um período de 3 meses. O procedimento de raspagem em campo aberto mostrou benefícios clínicos e microbiológicos no tratamento da peri-implantite e pode ser usado com segurança como grupo controle padrão para estudos futuros.

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Conference papers on the topic "Fusobacterium nucleatum"

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Garrett, Wendy S. "Abstract IA22: Fusobacterium nucleatum and colon cancer." In Abstracts: AACR Special Conference on Tumor Immunology and Immunotherapy; October 1-4, 2017; Boston, MA. American Association for Cancer Research, 2018. http://dx.doi.org/10.1158/2326-6074.tumimm17-ia22.

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Saputri, Dewi, Mahdi Abrar, Zaki Mubarak, and Mudatsir. "The Role of Fusobacterium Nucleatum on Chronic Periodontitis (Literature Review)." In 1st Aceh International Dental Meeting (AIDEM 2019), Oral Health International Conference On Art, Nature And Material Science Development 2019. Atlantis Press, 2021. http://dx.doi.org/10.2991/ahsr.k.210201.004.

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Zelcbuch, Lior, Sagit Yahav, Nufar Buchshtab, et al. "Abstract PR07: Novel phages targeting the intratumor-associated bacteria Fusobacterium nucleatum." In Abstracts: AACR Special Conference on the Microbiome, Viruses, and Cancer; February 21-24, 2020; Orlando, FL. American Association for Cancer Research, 2020. http://dx.doi.org/10.1158/1538-7445.mvc2020-pr07.

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Gearges, C., A. Kewalramani, M. Ali, and Z. Kuschner. "An Atypical Case of Fusobacterium Nucleatum Empyema Leading to Septic Shock." In American Thoracic Society 2023 International Conference, May 19-24, 2023 - Washington, DC. American Thoracic Society, 2023. http://dx.doi.org/10.1164/ajrccm-conference.2023.207.1_meetingabstracts.a5357.

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Silva, Mateus Borges da, JOÃO CARLOS DA SILVA, and TATIANA TEIXEIRA DE MIRANDA. "A BACTÉRIA FUSOBACTERIUM NUCLEATUM E O CÂNCER COLORRETAL: UMA REVISÃO INTEGRATIVA." In VI Congresso Brasileiro de Saúde. Revista Multidisciplinar em Saúde, 2025. https://doi.org/10.51161/vi-conbrasau/49854.

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Sakamoto, Yuki, Kosuke Mima, Nobuya Daitoku, et al. "Abstract 2837: Fusobacterium nucleatum and T cells in colorectal cancer liver metastasis." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.sabcs18-2837.

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Sakamoto, Yuki, Kosuke Mima, Nobuya Daitoku, et al. "Abstract 2837: Fusobacterium nucleatum and T cells in colorectal cancer liver metastasis." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.am2019-2837.

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Baba, Yoshifumi, Kensuke Yamamura, Shigeki Nakagawa, et al. "Abstract 4930: Genetic and epigenetic characteristics of esophageal cancer tissues with microbiome fusobacterium nucleatum." In Proceedings: AACR Annual Meeting 2017; April 1-5, 2017; Washington, DC. American Association for Cancer Research, 2017. http://dx.doi.org/10.1158/1538-7445.am2017-4930.

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Withers, Nathan J., Arjun Senthil, Marek Osinski, et al. "Effects of iron-oxide nanoparticles on compound biofilms of streptococcus gordonii and fusobacterium nucleatum." In Colloidal Nanoparticles for Biomedical Applications XIII, edited by Xing-Jie Liang, Wolfgang J. Parak, and Marek Osiński. SPIE, 2018. http://dx.doi.org/10.1117/12.2299280.

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Yudistira, Sisca, Dr Widyawati, and Resti Iswani. "Antibacterial Activities of Extract Curly Chili (Capsicumannum. L) on Fusobacterium Nucleatum: An in Vitro Study." In International Dental Conference of Sumatera Utara 2017 (IDCSU 2017). Atlantis Press, 2018. http://dx.doi.org/10.2991/idcsu-17.2018.68.

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