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Ganesan, Kumar, Songhe Guo, Sundaz Fayyaz, Ge Zhang, and Baojun Xu. "Targeting Programmed Fusobacterium nucleatum Fap2 for Colorectal Cancer Therapy." Cancers 11, no. 10 (2019): 1592. http://dx.doi.org/10.3390/cancers11101592.
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Colorectal patients generally have the maximum counts of Fusobacterium nucleatum (F. nucleatum) in tumors and elevate colorectal adenomas and carcinomas, which show the lowest rate of human survival. Hence, F. nucleatum is a diagnostic marker of colorectal cancer (CRC). Studies demonstrated that targeting fusobacterial Fap2 or polysaccharide of the host epithelium may decrease fusobacteria count in the CRC. Attenuated F. nucleatum-Fap2 prevents transmembrane signals and inhibits tumorigenesis inducing mechanisms. Hence, in this review, we hypothesized that application of genetically programmed fusobacterium can be skillful and thus reduce fusobacterium in the CRC. Genetically programmed F. nucleatum is a promising antitumor strategy.
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Han, Yiping W., Akihiko Ikegami, Chythanya Rajanna, et al. "Identification and Characterization of a Novel Adhesin Unique to Oral Fusobacteria." Journal of Bacteriology 187, no. 15 (2005): 5330–40. http://dx.doi.org/10.1128/jb.187.15.5330-5340.2005.
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ABSTRACT Fusobacterium nucleatum is a gram-negative anaerobe that is prevalent in periodontal disease and infections of different parts of the body. The organism has remarkable adherence properties, binding to partners ranging from eukaryotic and prokaryotic cells to extracellular macromolecules. Understanding its adherence is important for understanding the pathogenesis of F. nucleatum. In this study, a novel adhesin, FadA (Fusobacterium adhesin A), was demonstrated to bind to the surface proteins of the oral mucosal KB cells. FadA is composed of 129 amino acid (aa) residues, including an 18-aa signal peptide, with calculated molecular masses of 13.6 kDa for the intact form and 12.6 kDa for the secreted form. It is highly conserved among F. nucleatum, Fusobacterium periodonticum, and Fusobacterium simiae, the three most closely related oral species, but is absent in the nonoral species, including Fusobacterium gonidiaformans, Fusobacterium mortiferum, Fusobacterium naviforme, Fusobacterium russii, and Fusobacterium ulcerans. In addition to FadA, F. nucleatum ATCC 25586 and ATCC 49256 also encode two paralogues, FN1529 and FNV2159, each sharing 31% identity with FadA. A double-crossover fadA deletion mutant, F. nucleatum 12230-US1, was constructed by utilizing a novel sonoporation procedure. The mutant had a slightly slower growth rate, yet its binding to KB and Chinese hamster ovarian cells was reduced by 70 to 80% compared to that of the wild type, indicating that FadA plays an important role in fusobacterial colonization in the host. Furthermore, due to its uniqueness to oral Fusobacterium species, fadA may be used as a marker to detect orally related fusobacteria. F. nucleatum isolated from other parts of the body may originate from the oral cavity.
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Allen-Vercoe, Emma, Jaclyn Strauss, and Kris Chadee. "Fusobacterium nucleatum." Gut Microbes 2, no. 5 (2011): 294–98. http://dx.doi.org/10.4161/gmic.2.5.18603.
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Bashir, Arif, Abid Y. Miskeen, Ashaqullah Bhat, Khalid M. Fazili, and Bashir A. Ganai. "Fusobacterium nucleatum." European Journal of Cancer Prevention 24, no. 5 (2015): 373–85. http://dx.doi.org/10.1097/cej.0000000000000116.
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Yocum, Denise. "Fusobacterium nucleatum." Journal of the American Academy of Physician Assistants 29, no. 12 (2016): 1–4. http://dx.doi.org/10.1097/01.jaa.0000508216.58368.74.
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Dussmann, Heiko, Barry Maguire, Caoimbhe Burke, et al. "Abstract 6531: High resolution analysis of Fusobacterium infection in colorectal cancer." Cancer Research 85, no. 8_Supplement_1 (2025): 6531. https://doi.org/10.1158/1538-7445.am2025-6531.
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Previous research provided evidence pointing towards an important role for bacterial infection with Fusobacterium nucleatum (F. nucleatum) and F. animalis (previuosly F. nucleatum subsp. animalis) in colorectal cancer (CRC) progression. Both are oral commensal Gram negative anaerobes with recent evidence suggesting F. animalis predominates in CRC (Zepeda-Rivera et al., 2024). We recently applied spatial transcriptomics to demonstrate local effects of Fusobacterium infection on cancer and immune cell gene expression in CRC (Duggan et al, Gut Microbes, 2024). While Fusobacterium infection was observed in specific patches in the tumors, the precise cell types infected with bacteria and the precise localization of bacteria in cells still requires further elucidation. Commercially available strains of F. nucleatum (ATCC 25586) and F. animalis (ATCC 51191) were purchased and separately co-cultured with MDA-MB-468 breast cancer cells. Immuno cytochemistry using a commercial Fusobacteria antibody (ANT0084) and a Fusobacteria antiserum (Prof Slade group), along with pancytokeratin and Hoechst staining was undertaken. High resolution confocal microscopy using an LSM 980 Airyscan 2 microscope (Carl Zeiss, Jena, Germany) was performed to create image stacks of optical sections. CRC tissue sections were subsequently prepared and stained by immunohistochemistry (IHC) for pancytokeratin, CD68, and Fusobacteria (Slade antisera). Imaging of the full tumor face was undertaken at a lower resolution using Cell DIVE (Leica Microsystems, Wetzlar, Germany) to select regions rich in Fusobacteria for further study. Selected regions were then imaged using high resolution confocal microscopy. Fusobacteria antisera stained both F. nucleatum and F. animalis while the commercially produced antibody stained only F. nucleatum. Both bacteria can populate the cell surroundings, cell surface, cytoplasm as well as nucleus. The typical morphology of both F. nucleatum and F. animalis is seen, allowing antibody validation. In CRC tissue intracellular bacteria are seen in both pancytokeratin positive tumor epithelial cells as well as in CD68 (Cluster of Differentiation 68) positive cells (tumor associated macrophages). Tumor associated macrophages (TAMs) have an important role in the tumor immmune microenvironment. In particular, studies have highlighted the importance of M1/M2 macrophage polarization on immune-evasion and prognosis. Our spatial transcriptomic study highlighted reduced M2 polarization as associated with increased Fusobacterial load. The intracellular location of Fusobacteria may suggest a mechanism for altered macrophage polarization. References: Zepeda-Rivera et al, Nature Vol 628, pages 424-432 (2024)Flanagan et al, Eur J Clin Microbiol Infect Dis. 2014 Aug;33(8):1381-90 Duggan et al, Gut Microbes. 2024 Jan-Dec;16(1):2350149. Citation Format: Heiko Dussmann, Barry Maguire, Caoimbhe Burke, Arman Raman, John Burke, William M. Gallagher, Jochen H. Prehn. High resolution analysis of Fusobacterium infection in colorectal cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2025; Part 1 (Regular Abstracts); 2025 Apr 25-30; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2025;85(8_Suppl_1):Abstract nr 6531.
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Mandal, Devi Prasad, Neeta Mohanty, Paresh Kumar Behera, et al. "A Plausible Proposition of CCL20-Related Mechanism in Fusobacterium nucleatum-Associated Oral Carcinogenesis." Life 11, no. 11 (2021): 1218. http://dx.doi.org/10.3390/life11111218.
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Objective: The objective of this prospective observational case–control study is to evaluate the prevalence of Fusobacterium nucleatum in the tissues of oral squamous cell carcinoma (OSCC). Reconnoitering the CCL20-related mechanism of carcinogenesis in Fusobacterium nucleatum-positive OSCC is another objective. Methodology: Tissues from 50 OSCC patients and 30 healthy oral tissues were collected. The prevalence of Fusobacterium nucleatum was evaluated in both tumour and healthy tissue by polymerase chain reaction. The immunohistochemistry of OSCC tissues was conducted to evaluate the difference in the expression of CCL20 between Fusobacterium nucleatum-positive and -negative OSCC tissues. Results: Fusobacterium nucleatum was significantly (p < 0.001) prevalent in OSCC tissues (74%), compared to healthy tissues (26%). No association of Fusobacterium nucleatum or CCL20 immuno-expression with any clinical or histopathological features of OSCC was observed. While the intensity of CCL20 immuno-expression did not differ (p = 0.053), the CCL20-positive cell population was significantly different (p = 0.034) between Fusobacterium nucleatum-positive and -negative OSCC. Conclusion: Fusobacterium nucleatum is possibly prevalent in oral cancer tissues in the Indian population. By using immunohistochemistry, this is the first study to propose that the carcinogenesis in Fusobacterium nucleatum-positive OSCC may be CCL20-related. The findings enrich the knowledge of mechanisms involved in Fusobacterium nucleatum-mediated oral carcinogenesis.
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Beavers, Bruce R. "FUSOBACTERIUM NUCLEATUM PYOMYOSITIS." Orthopedics 15, no. 2 (1992): 208–11. http://dx.doi.org/10.3928/0147-7447-19920201-17.
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Bachrach, Gilad, Susan Kinder Haake, Alon Glick, et al. "Characterization of the Novel Fusobacterium nucleatum Plasmid pKH9 and Evidence of an Addiction System." Applied and Environmental Microbiology 70, no. 12 (2004): 6957–62. http://dx.doi.org/10.1128/aem.70.12.6957-6962.2004.
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ABSTRACT Fusobacterium nucleatum is an important oral anaerobic pathogen involved in periodontal and systemic infections. Studies of the molecular mechanisms involved in fusobacterial virulence and adhesion have been limited by lack of systems for efficient genetic manipulation. Plasmids were isolated from eight strains of F. nucleatum. The smallest plasmid, pKH9 (4,975 bp), was characterized and used to create new vectors for fusobacterial genetic manipulation. DNA sequence analysis of pKH9 revealed an open reading frame (ORF) encoding a putative autonomous rolling circle replication protein (Rep), an ORF predicted to encode a protein homologous to members of the FtsK/SpoIIIE cell division-DNA segregation protein family, and an operon encoding a putative toxin-antitoxin plasmid addiction system (txf-axf). Deletion analysis localized the pKH9 replication region in a 0.96-kbp fragment. The pKH9 rep gene is not present in this fragment, suggesting that pKH9 can replicate in fusobacteria independently of the Rep protein. A pKH9-based, compact Escherichia coli-F. nucleatum shuttle plasmid was constructed and found to be compatible with a previously described pFN1-based fusobacterial shuttle plasmid. Deletion of the pKH9 putative addiction system (txf-axf) reduced plasmid stability in fusobacteria, indicating its addiction properties and suggesting it to be the first plasmid addiction system described for fusobacteria. pKH9, its genetic elements, and its shuttle plasmid derivatives can serve as useful tools for investigating fusobacterial properties important in biofilm ecology and pathogenesis.
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Shhadeh, Amjad, Liat Dassa, Jamal Fahoum, et al. "Abstract 5893: Suppression of anti-tumor immunity by the Fusobacterium nucleatum protease fusolisin." Cancer Research 83, no. 7_Supplement (2023): 5893. http://dx.doi.org/10.1158/1538-7445.am2023-5893.
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Abstract Fusobacterium nucleatum is an oral pathogen associated with periodontal disease, preterm births and the exacerbation of colorectal, esophageal, pancreatic, and breast cancers. F. nucleatum was previously found to inhibit killing of cancer cells by natural killer (NK) cells and tumor infiltrating T cells by inducing the NK cells killing-suppressing receptors TIGIT and CEACAM1. In this study, we show that the incubation of F. nucleatum with primary human natural killer (NK) cells causes the cleavage of the activating receptors CD16, NKp44 and NKp46 by fusolisin. Fusolisin is a fusobacterial outer-membrane, auto-transporter, serine protease and to date the only functional protease found in F. nucleatum. We previously showed that fusolisin is essential for fusobacterial growth in culture. High expression of a functional recombinant fusolisin in E. coli was enabled using codon optimization and replacement of the fusolisin's signal peptide with that of the E. coli OmpA. Recombinant fusolisin was found to cleave the same NK-activating receptors degraded by F. nucleatum. Cleavage of these activating receptors by fusolisin inhibited NK cells activity including killing of tumor cells in-vitro and in-vivo. Our results provide a new bacterial protease-dependent mechanism in which tumors colonized by F. nucleatum are protected from NK cells attack by exploiting fusolisin proteolytic activity. Importantly, our previous and current results suggest that fusolisin might serve to target for tumor-colonized fusobacteria. Citation Format: Amjad Shhadeh, Liat Dassa, Jamal Fahoum, Nicole Haj, Reuven Wiener, Ofer Mandelboim, Gilad Bachrach. Suppression of anti-tumor immunity by the Fusobacterium nucleatum protease fusolisin [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 5893.
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Gharbia, S. E., and H. N. Shah. "Fusobacterium nucleatum subsp. fusiforme subsp. nov. and Fusobacterium nucleatum subsp. animalis subsp. nov. as Additional Subspecies within Fusobacterium nucleatum." International Journal of Systematic Bacteriology 42, no. 2 (1992): 296–98. http://dx.doi.org/10.1099/00207713-42-2-296.
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Suehiro, Yutaka, Kouhei Sakai, Mitsuaki Nishioka, et al. "Highly sensitive stool DNA testing of Fusobacterium nucleatum as a marker for detection of colorectal tumours in a Japanese population." Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 54, no. 1 (2016): 86–91. http://dx.doi.org/10.1177/0004563216643970.
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Background Accumulating evidence shows an over-abundance of Fusobacterium nucleatum in colorectal tumour tissues. Although stool DNA testing of Fusobacterium nucleatum might be a potential marker for the detection of colorectal tumours, the difficulty in detecting Fusobacterium nucleatum in stool by conventional methods prevented further explorations. Therefore, we developed a droplet digital polymerase chain reaction (PCR) assay for detecting Fusobacterium nucleatum in stool and investigated its clinical utility in the management of colorectal tumours in a Japanese population. Methods Feces were collected from 60 healthy subjects (control group) and from 11 patients with colorectal non-advanced adenomas (non-advanced adenoma group), 19 patients with colorectal advanced adenoma/carcinoma in situ (advanced adenoma/carcinoma in situ (CIS) group) and 158 patients with colorectal cancer of stages I to IV (colorectal cancer group). Absolute copy numbers of Fusobacterium nucleatum were measured by droplet digital PCR. Results The median copy number of Fusobacterium nucleatum was 17.5 in the control group, 311 in the non-advanced adenoma group, 122 in the advanced adenoma/CIS group, and 317 in the colorectal cancer group. In comparison with that in the control group, the Fusobacterium nucleatum level was significantly higher in the non-advanced adenoma group, the advanced adenoma/CIS group and the colorectal cancer group. Conclusions This study illustrates the potential of stool DNA testing of Fusobacterium nucleatum by droplet digital PCR to detect individuals with colorectal tumours in a Japanese population.
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Yeoh, Yun Kit, Zigui Chen, Martin C. S. Wong, et al. "Southern Chinese populations harbour non-nucleatum Fusobacteria possessing homologues of the colorectal cancer-associated FadA virulence factor." Gut 69, no. 11 (2020): 1998–2007. http://dx.doi.org/10.1136/gutjnl-2019-319635.
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ObjectiveFusobacteria are not common nor relatively abundant in non-colorectal cancer (CRC) populations, however, we identified multiple Fusobacterium taxa nearly absent in western and rural populations to be comparatively more prevalent and relatively abundant in southern Chinese populations. We investigated whether these represented known or novel lineages in the Fusobacterium genus, and assessed their genomes for features implicated in development of cancer.MethodsPrevalence and relative abundances of fusobacterial species were calculated from 3157 CRC and non-CRC gut metagenomes representing 16 populations from various biogeographies. Microbial genomes were assembled and compared with existing reference genomes to assess novel fusobacterial diversity. Phylogenetic distribution of virulence genes implicated in CRC was investigated.ResultsIrrespective of CRC disease status, southern Chinese populations harboured increased prevalence (maximum 39% vs 7%) and relative abundances (average 0.4% vs 0.04% of gut community) of multiple recognised and novel fusobacterial taxa phylogenetically distinct from Fusobacterium nucleatum. Genomes assembled from southern Chinese gut metagenomes increased existing fusobacterial diversity by 14.3%. Homologues of the FadA adhesin linked to CRC were consistently detected in several monophyletic lineages sister to and inclusive of F. varium and F. ulcerans, but not F. mortiferum. We also detected increased prevalence and relative abundances of F. varium in CRC compared with non-CRC cohorts, which together with distribution of FadA homologues supports a possible association with gut disease.ConclusionThe proportion of fusobacteria in guts of southern Chinese populations are higher compared with several western and rural populations in line with the notion of environment/biogeography driving human gut microbiome composition. Several non-nucleatum taxa possess FadA homologues and were enriched in CRC cohorts; whether this imposes a risk in developing CRC and other gut diseases deserves further investigation.
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Varela Barca, Laura, Javier Miguelena Hycka, Javier Cobo Reinoso, and Jose Romero Vivas. "Endocarditis por Fusobacterium nucleatum." Revista Colombiana de Cardiología 24, no. 5 (2017): 539–40. http://dx.doi.org/10.1016/j.rccar.2017.05.009.
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Hockensmith, M. L., D. L. Mellman, and E. L. Aronsen. "Fusobacterium nucleatum Empyema Necessitans." Clinical Infectious Diseases 29, no. 6 (1999): 1596–98. http://dx.doi.org/10.1086/313553.
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Hsieh, Yung-Yu, Wen-Lin Kuo, Wan-Ting Hsu, Shui-Yi Tung, and Chin Li. "Fusobacterium Nucleatum-Induced Tumor Mutation Burden Predicts Poor Survival of Gastric Cancer Patients." Cancers 15, no. 1 (2022): 269. http://dx.doi.org/10.3390/cancers15010269.
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Co-infection of Helicobacter pylori and Fusobacterium nucleatum is a microbial biomarker for poor prognosis of gastric cancer patients. Fusobacterium nucleatum is associated with microsatellite instability and the accumulation of mutations in colorectal cancer. Here, we investigated the mutation landscape of Fusobacterium nucleatum-positive resected gastric cancer tissues using Illumina TruSight Oncology 500 comprehensive panel. Sequencing data were processed to identify the small nucleotide variants, small insertions and deletions, and unstable microsatellite sites. The bioinformatic algorithm also calculated copy number gains of preselected genes and tumor mutation burden. The recurrent genetic aberrations were identified in this study cohort. For gene amplification events, ERBB2, cell cycle regulators, and specific FGF ligands and receptors were the most frequently amplified genes. Pathogenic activation mutations of ERBB2, ERBB3, and PIK3CA, as well as loss-of-function of TP53, were identified in multiple patients. Furthermore, Fusobacterium nucleatum infection is positively correlated with a higher tumor mutation burden. Survival analysis showed that the combination of Fusobacterium nucleatum infection and high tumor mutation burden formed an extremely effective biomarker to predict poor prognosis. Our results indicated that the ERBB2-PIK3-AKT-mTOR pathway is frequently activated in gastric cancer and that Fusobacterium nucleatum and high mutation burden are strong biomarkers of poor prognosis for gastric cancer patients.
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Zundler, Sebastian, Christian Mardin, Simone Bertz, et al. "Rectal Cancer Presenting with Absceding Infection Due to Fusobacterium nucleatum." Pathogens 11, no. 10 (2022): 1113. http://dx.doi.org/10.3390/pathogens11101113.
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Intestinal microbiota such as Fusobacterium nucleatum play an important role in the pathogenesis of colorectal cancer. Here, we describe the case of a 47-year-old patient presenting with endophthalmitis and a liver abscess due to Fusobacterium nucleatum that prompted the diagnosis of colorectal cancer as the most likely source of infection. This case highlights that colorectal cancer needs to be considered in patients with systemic infection with Fusobacterium nucleatum and colonoscopy should be performed.
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Edwards, Andrew M., Tracy J. Grossman, and Joel D. Rudney. "Fusobacterium nucleatum Transports Noninvasive Streptococcus cristatus into Human Epithelial Cells." Infection and Immunity 74, no. 1 (2006): 654–62. http://dx.doi.org/10.1128/iai.74.1.654-662.2006.
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ABSTRACT Analysis of human buccal epithelial cells frequently reveals an intracellular polymicrobial consortium of bacteria. Although several oral bacteria have been demonstrated to invade cultured epithelial cells, several others appear unable to internalize. We hypothesized that normally noninvasive bacteria may gain entry into epithelial cells via adhesion to invasive bacteria. Fusobacterium nucleatum is capable of binding to and invading oral epithelial cells. By contrast, Streptococcus cristatus binds weakly to host cells and is not internalized. F. nucleatum and S. cristatus coaggregate strongly via an arginine-sensitive interaction. Coincubation of KB or TERT-2 epithelial cells with equal numbers of F. nucleatum and S. cristatus bacteria led to significantly increased numbers of adherent and internalized streptococci. F. nucleatum also promoted invasion of KB cells by other oral streptococci and Actinomyces naeslundii. Dissection of fusobacterial or streptococcal adhesive interactions by using sugars, amino acids, or antibodies demonstrated that this phenomenon is due to direct attachment of S. cristatus to adherent and invading F. nucleatum. Inhibition of F. nucleatum host cell attachment and invasion with galactose, or fusobacterial-streptococcal coaggregation by the arginine homologue l-canavanine, abrogated the increased S. cristatus adhesion to, and invasion of, host cells. In addition, polyclonal antibodies to F. nucleatum, which inhibited fusobacterial attachment to both KB cells and S. cristatus, significantly decreased invasion by both species. Similar decreases were obtained when epithelial cells were pretreated with cytochalasin D, staurosporine, or cycloheximide. These studies indicate that F. nucleatum may facilitate the colonization of epithelial cells by bacteria unable to adhere or invade directly.
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Nonutu, Stevia E., Damajanty H. C. Pangemanan, and Christy N. Mintjelungan. "Uji Daya Hambat Ekstrak Ikan Nike (Awous melanocephalus) Terhadap Pertumbuhan Bakteri Fusobacterium nucleatum." e-GiGi 9, no. 2 (2021): 238. http://dx.doi.org/10.35790/eg.v9i2.34982.
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Abstract: One of the treatment options of periodontal abscess caused by Fusobacterium nucleatum is administration of antibiotics. However, long-term antibiotics consumption can cause negative side effects. Therefore, alternative treatments that have low side effects and easy to be obtained are needed. Nike fish (Awaous melanocephalus) is one of the endemic fish of North Sulawesi province which has antibacterial properties. This study was aimed to evaluate the inhibition effect of nike fish extract on the growth of Fusobacterium nucleatum. This was a true experimental study with a posttest only control group design. We used modified Kirby-Bauer method with filter papers. Ciprofloxacin was used as the positive control and aquadest as the negative control. Extract of nike fish and stock of pure bacteria Fusobacterium nucleatum were prepared. The results showed that the average diameters of the inhibition zones formed in the nike fish extract after three repetitions, were as follows: for extract concentration of 12.5% was 2.91 mm; 25% was 4.16 mm; 50% was 8.41 mm; and 100% was 9.58 mm. In conclusion, nike fish extract (Awaous melanocephalus) at concentrations of 50% and 100% had a weak inhibitory effect (Himedia category) on the growth of Fusobacterium nucleatum meanwhile at concentrations of 12.5% and 25% there was no activity of zone of inhibition.Keywords: extract of nike fish (Awaous melanocephalus); Fusobacterium nucleatum; inhibitory effect Abstrak: Salah satu opsi pengobatan abses periodontal yang disebabkan oleh bakteri Fusobacterium nucleatum yaitu dengan penggunaan antibiotik namun mengonsumsi antibiotik jangka panjang dapat menimbulkan efek samping negatif. Oleh karena itu, diperlukan pengobatan alternatif yang memiliki efek samping rendah serta mudah didapat. Ikan nike merupakan salah satu ikan endemik Provinsi Sulawesi Utara yang berkhasiat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak ikan nike (Awaous melanocephalus) terhadap pertumbuhan bakteri Fusobacterium nucleatum. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Metode yang digunakan yaitu metode modifikasi Kirby-Bauer dengan menggunakan paper disk. Kontrol positif menggunakan antibakteri ciprofloxacin dan kontrol negatif menggunakan akuades. Pada penelitian ini digunakan ekstrak ikan nike dan stok bakteri murni Fusobacterium nucleatum. Hasil penelitian menunjukkan bahwa rerata diameter zona hambat yang terbentuk pada ekstrak ikan nike setelah tiga kali pengulangan yaitu untuk konsentrasi 12,5% sebesar 2,91 mm; 25% sebesar 4,16 mm; 50% sebesar 8,41 mm; dan 100% sebesar 9,58 mm. Simpulan penelitian ini ialah ekstrak ikan nike (Awaous melanocephalus) pada konsentrasi 50% dan 100% memiliki daya hambat kategori lemah (Himedia) terhadap pertumbuhan bakteri Fusobacterium nucleatum sedangkan pada konsentrasi 12,5% dan 25% dikategorikan tidak terdapat aktivitas zona hambat. Kata kunci: ekstrak ikan nike (Awaous melanocephalus); Fusobacterium nucleatum; daya hambat
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Gaba, Fariah I., Raquel Carcelén González, and Raquel González Martïnez. "The Role of Oral Fusobacterium nucleatum in Female Breast Cancer: A Systematic Review and Meta-Analysis." International Journal of Dentistry 2022 (November 23, 2022): 1–12. http://dx.doi.org/10.1155/2022/1876275.
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Introduction. Breast cancer is the world’s most prevalent malignancy, with an increasing incidence and a predisposition for postpubertal females from all cultural and ethnic backgrounds. More recently, oral Fusobacterium nucleatum species have been observed in cancerous human breast tissue, drawing attention to the role of microbes in cancer pathogenesis. Objectives. Investigating oral Fusobacterium nucleatum species as potential biomarkers for female-specific breast cancer. Methods. A systematic search in The Central Register of Controlled Trials, EMBASE, EBSCO, NCBI, and MEDLINE databases was undertaken from the 1st January, 1983–31st March, 2022. Articles included were in English and based on women between the ages of 18–96 years with confirmed gingivitis/periodontal disease and breast cancer diagnoses from registered specialists. Authors extracted data independently, and a meta-analysis of risk estimations measuring associations between oral Fusobacterium nucleatum species and female-specific breast cancer was elucidated via calculated relative risks and 95% confidence intervals. Results. AXIS tool analysis revealed 78.70% of articles with a positive correlation between oral Fusobacterium nucleatum and female-specific breast cancer. The risk of breast cancer development increased with significant levels of oral Fusobacterium nucleatum due to gingivitis/periodontitis (relative risk = 1.78, 95% confidence interval = 1.63–1.91). Low-moderate statistical heterogeneity was found (I2 = 41.39%; P = 0.02), and the importance of periodontal status on breast cancer pathogenesis was determined (relative risk = 1.24, 95% confidence interval = 1.01–1.30). Conclusions. Oral Fusobacterium nucleatum species are a risk factor for breast cancer development, thus elevating their biomarker potentiality.
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Șurlin, Petra, Flavia Mirela Nicolae, Valeriu Marin Șurlin, et al. "Could Periodontal Disease through Periopathogen Fusobacterium nucleatum Be an Aggravating Factor for Gastric Cancer?" Journal of Clinical Medicine 9, no. 12 (2020): 3885. http://dx.doi.org/10.3390/jcm9123885.
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Periodontal disease affects the supporting tissues of the teeth, being a chronic inflammatory disease caused by specific microorganisms from subgingival biofilm. Fusobacterium nucleatum is a Gram-negative anaerobic bacterium that acts as a periodontal pathogen, being an important factor in linking Gram-positive and Gram-negative bacteria in the periodontal biofilm, but its involvement in systemic diseases has also been found. Several studies regarding the implication of Fusobacterium nucleatum in gastro-enterological cancers have been conducted. The present review aims to update and systematize the latest information about Fusobacterium nucleatum in order to evaluate the possibility of an association between periodontal disease and the evolution of gastroenterological cancers through the action of Fusobacterium nucleatum, highlighting gastric cancer. This would motivate future research on the negative influence of periodontal pathology on the evolution of gastric cancer in patients suffering from both pathologies.
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Rosen, Graciela, Ira Nisimov, Monica Helcer, and Michael N. Sela. "Actinobacillus actinomycetemcomitans Serotype b Lipopolysaccharide Mediates Coaggregation with Fusobacterium nucleatum." Infection and Immunity 71, no. 6 (2003): 3652–56. http://dx.doi.org/10.1128/iai.71.6.3652-3656.2003.
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ABSTRACT Purified Actinobacillus actinomycetemcomitans serotype b lipopolysaccharide (LPS) was found to be able to bind Fusobacterium nucleatum cells and to inhibit binding of F. nucleatum to A. actinomycetemcomitans serotype b. Sugar binding studies showed that the requirements for binding of A. actinomycetemcomitans serotype b LPS to the F. nucleatum lectin are the presence of a metal divalent ion, an axial free hydroxyl group at position 4, and free equatorial hydroxyl groups at positions 3 and 6 of d-galactose, indicating that the β-N-acetyl-d-galactosamine in the serotype b LPS trisaccharide repeating unit is the monosaccharide residue recognized by the F. nucleatum lectin. These data strongly suggest that A. actinomycetemcomitans serotype b LPS is one of the receptors responsible for the lactose-inhibitable coaggregation of A. actinomycetemcomitans to fusobacteria.
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Stokowa-Sołtys, Kamila, Kamil Wojtkowiak, and Karolina Jagiełło. "Fusobacterium nucleatum – Friend or foe?" Journal of Inorganic Biochemistry 224 (November 2021): 111586. http://dx.doi.org/10.1016/j.jinorgbio.2021.111586.
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Li, Rongrong, Jilu Shen, and Yuanhong Xu. "Fusobacterium nucleatum and Colorectal Cancer." Infection and Drug Resistance Volume 15 (March 2022): 1115–20. http://dx.doi.org/10.2147/idr.s357922.
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Haber, Stuart W. "Splenic Abscess from Fusobacterium nucleatum." Annals of Internal Medicine 110, no. 11 (1989): 948. http://dx.doi.org/10.7326/0003-4819-110-11-948_1.
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Merritt, Justin, Guoqing Niu, Toshinori Okinaga, and Felicia Qi. "Autoaggregation Response of Fusobacterium nucleatum." Applied and Environmental Microbiology 75, no. 24 (2009): 7725–33. http://dx.doi.org/10.1128/aem.00916-09.
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ABSTRACT Fusobacterium nucleatum is a gram-negative oral bacterial species associated with periodontal disease progression. This species is perhaps best known for its ability to adhere to a vast array of other bacteria and eukaryotic cells. Numerous studies of F. nucleatum have examined various coaggregation partners and inhibitors, but it is largely unknown whether these interactions induce a particular genetic response. We tested coaggregation between F. nucleatum ATCC strain 25586 and various species of Streptococcus in the presence of a semidefined growth medium containing saliva. We found that this condition could support efficient coaggregation but, surprisingly, also stimulated a similar degree of autoaggregation. We further characterized the autoaggregation response, since few reports have examined this in F. nucleatum. After screening several common coaggregation inhibitors, we identified l-lysine as a competitive inhibitor of autoaggregation. We performed a microarray analysis of the planktonic versus autoaggregated cells and found nearly 100 genes that were affected after only about 60 min of aggregation. We tested a subset of these genes via real-time reverse transcription-PCR and confirmed the validity of the microarray results. Some of these genes were also found to be inducible in cell pellets created by centrifugation. Based upon these data, it appears that autoaggregation activates a genetic program that may be utilized for growth in a high cell density environment, such as the oral biofilm.
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Tweedy, Charles R., and William B. White. "Multiple Fusobacterium Nucleatum Liver Abscesses." Journal of Clinical Gastroenterology 9, no. 2 (1987): 194–97. http://dx.doi.org/10.1097/00004836-198704000-00017.
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Gholizadeh, Pourya, Hosein Eslami, and Hossein Samadi Kafil. "Carcinogenesis mechanisms of Fusobacterium nucleatum." Biomedicine & Pharmacotherapy 89 (May 2017): 918–25. http://dx.doi.org/10.1016/j.biopha.2017.02.102.
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FREDRIKSEN, GJERT, and TOR HOFSTAD. "CHEMOTYPES OF FUSOBACTERIUM NUCLEATUM LIPOPOLYSACCHARIDES." Acta Pathologica Microbiologica Scandinavica Section B Microbiology 86B, no. 1-6 (2009): 41–46. http://dx.doi.org/10.1111/j.1699-0463.1978.tb00006.x.
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Ireh, Choe. "ASSOCIATION OF FUSOBACTERIUM NUCLEATUM WITH HALITOSIS." INTERNATIONAL EDUCATIONAL JOURNAL OF SCIENCE AND ENGINEERING - IEJSE 7, no. 10 (2024): 20–22. https://doi.org/10.5281/zenodo.15608652.
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This paper focuses on 3 main negative effects of <em>Fusobacterium nucleatum</em> on halitosis. Halitosis, which is known as bad breath, is a common disease among people. <em>Fusobacterium nucleatum</em> produces VSCs, such as hydrogen sulfide and methyl mercaptan, which cause halitosis. Another effect of <em>Fusobacterium nucleatum</em> is that it triggers periodontal disease to induce halitosis. The microbe also collaborates with other bacteria like <em>Porphyromonas gingivalis</em> and <em>Capnocytophaga ochracea</em> to leave a negative influence on bad breath. It is important to recognize this issue in order to further examine and create future treatments.
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Mela, V., A. Pantazatou, A. Tsakris, et al. "Comparative evaluation of selective culture and PCR for detection of Fusobacterium necrophorum and Fusobacterium nucleatum in throat specimens." ACTA MICROBIOLOGICA HELLENICA 61, no. 4 (2016): 285–90. https://doi.org/10.5281/zenodo.10066672.
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Culture on selective media (Fusobacteriumselective agar supplemented with 4 mg/L vancomycin and 8 mg/L neomycin) was compared to PCR (rpoB and haem genes for Fusobacterium necrophorum, 16S rDNA fragment specific for Fusobacterium nucleatum) for direct detection of the species in throat specimens of 88 patients with sore throat and pharyngotonsilits. Among the 49 culture-positive specimens, culture and PCR revealed identical positive results in 38 (43.1%) and eight (9.1%) patients for F. nucleatum and F. necrophorum, respectively, whilst in two more patients (2.3%), both pathogens were detected by both methods. Only a single F. nucleatum culture-positive specimen was PCR-negative. In contrast, among the remaining 39 culture-negative specimens, five (5.7%) and two (2.3%) were positive by PCR for F. nucleatum and F. necrophorum, respectively. Sub-species identification revealed that all F. necrophorum isolates were identified as F. necrophorumssp. funduliforme. Sensitivity, specificity, PPV and NPV of the 16S rDNA PCR (F. nucleatum detection) were 97.6, 89.4, 88.9 and 97.7%, respectively, whilst the respective values for the rpoB PCR (F. necrophorumdetection) were 100.0, 97.4, 83.3 and 100.0%, as compared to culture.In conclusion, PCR proved of higher diagnostic yield than culture and detected both species in culture-negative specimens. As similar results have been obtained for other anaerobic species, the golden standard anaerobic culture seems to be insufficient for detecting Fusobacterium spp. in the area of molecular microbiology and needs further re-evaluation.
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Hakima, Anisa Nur, Tantin Ermawati, and Happy Harmono. "Daya Hambat Ekstrak Biji Kopi Robusta (Coffea robusta) terhadap Pertumbuhan Fusobacterium nucleatum." STOMATOGNATIC - Jurnal Kedokteran Gigi 15, no. 2 (2018): 43. http://dx.doi.org/10.19184/stoma.v15i2.17932.
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Fusobacterium nucleatum is a bacteria found in normal flora of the oral cavity that plays a role in the occurrence of periodontal disease. The prevalence of periodontal disease in Indonesia reaches 70%. One treatment by using medicinal plants is robusta coffee beans. Robusta coffee beans contain volatile acids, chlorogenic acids, caffeine acids, phenols and caffeine suspected to be antibacterial. The objective of this study was to determine the inhibition of coffee robusta extract on growth of Fusobacterium nucleatum. This study used disc diffusion method with 4 samples in each study group. The study group consisted of 6 treatment groups (12,5%, 25%, 50% and 100% coffee robusta extract), positive control group (chlorhexidine gluconate 0,2%), and negative control group (sterile aquades). Data were analyzed using One Way Anova test and LSD (Least Significant Difference) test. Coffee robusta extract has the ability to inhibit the growth of Fusobacterium nucleatum. The concentration of coffe robusta extract 100%, 50%, 25%, 12,5% have same ability to inhibit the growth of Fusobacterium nucleatum.
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Wantenia, Fenny, Chandra Susanto, and M. Suksestio W. "Pengaruh Strobilanthes Crispus BI terhadap KHM dan KBM pada bakteri A.Actinomycetemcomitans dan F.Nucleatum." Jurnal Ilmiah dan Teknologi Kedokteran Gigi 16, no. 1 (2020): 36. http://dx.doi.org/10.32509/jitekgi.v16i1.1012.
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ABSTRAK Latar belakang: Periodontitis merupakan suatu penyakit infeksi atau penyakit inflamasi yang dapat menyebabkan kerusakkan jaringan periodontal dan menyebabkan kerusakan pada tulang alveolar sehingga karena adanya penyakit periodontitis tersebut maka akan lebih rentan jika terjadinya kehilangan gigi. Adapun salah satu bakteri yang dapat memicu terjadinya penyakit periodontitis adalah bakteri Aggregatibacter Actinomycetemcomitans dan bakteri Fusobacterium Nucleatum yang merupkan bakteri gram negatif atau bakteri anaerob. Penyakit periodontitis dapat juga disembuhkan dengan menggunakan bahan alami seperti ekstrak daun Keji Beling (Strobilanthes Crispus BI) yang mengandung zat kimia seperti kalium, natrium, kalsium, asam silikat, flavonoida, dan polifenol. Tujuan: Tujuan dari penelitian ini adalah untuk mengetahui pengaruh ekstrak daun keji beling (Strobilanthes Crispus BI) terhadap kadar hambat minimum dan kadar bunuh minimum pada bakteri Aggregatibacter Actinomycetemcomitans dan bakteri Fusobacterium Nucleatum. Metode: Metode yang digunakan adalah metode dilusi dimana metode tersebut dilakukan didalam tabung reaksi. Hasil: Hasil penelitian menunjukkan bahwa pada perhitungan uji normalitas terhadap bakteri Aggregatibacter Actiomycetemcomitans terhadap ekstrak daun keji beling (Strobilanthes Crispus BI) tidak normal, sedangkan pada bakteri Fusobacterium Nucleatumterhadap ekstrak daun keji beling (Strobilanthes Crispus BI) terdistribusi normal. Pada perhitungan uji homogenitas didapatkan bahwa data kadar hambat dan kadar bunuh minimum pada bakteri Aggregatibacter Actiomycetemcomitans terhadap ekstrak daun keji beling (Strobilanthes Crispus BI) adalah tidak homogen, sedangkan pada bakteri Fusobacterium Nucleatum adalah homogen. Kesimpulan: Kesimpulan dari penlitian ini adalah ekstrak daun keji beling (Strobilanthes Crispus BI) dapat menghambat pertumbuhan pada bakteri Aggregatibacter Actinomycetemcomitans dan bakteri Fusobacterium Nucleatum. Kata kunci: Peridontitis, keji beling (Strobilanthes Crispus BI), Aggregatibacter Actinomycetemcomitans, Fusobacterium Nucleatum.
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Gendron, Renée, Pascale Plamondon, and Daniel Grenier. "Binding of Pro-Matrix Metalloproteinase 9 by Fusobacterium nucleatum subsp. nucleatum as a Mechanism To Promote the Invasion of a Reconstituted Basement Membrane." Infection and Immunity 72, no. 10 (2004): 6160–63. http://dx.doi.org/10.1128/iai.72.10.6160-6163.2004.
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ABSTRACT In this study, we investigated the ability of Fusobacterium nucleatum subsp. nucleatum to increase its tissue-invasive potential by acquiring cell-associated human matrix metalloproteinase 9 (MMP-9) activity. Binding of pro-MMP-9 to fusobacteria was demonstrated by enzyme-linked immunosorbent assay. Zymography and a colorimetric assay showed that bound pro-MMP-9 can be converted into a proteolytically active form. The potential contribution of this acquired host activity in tissue invasion was demonstrated using a reconstituted basement membrane (Matrigel).
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Gohar, Ahmed, Fady Jamous, and Mohamed Abdallah. "Concurrent fusobacterial pyogenic liver abscess and empyema." BMJ Case Reports 12, no. 10 (2019): e231994. http://dx.doi.org/10.1136/bcr-2019-231994.
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We present a very rare case of concurrent empyema and liver abscess caused by Fusobacterium. Our patient presented with 3-month history of subtle abdominal discomfort and cough leading to eventually presenting with marked chest pain, dyspnoea and septic shock. CT revealed a liver abscess and large right-sided pleural effusion. Drainage of the pleural effusion yielded gross pus with the growth of Fusobacterium varium, while drainage of the liver abscess yielded Fusobacterium nucleatum. The patient responded to drainage and antibiotic therapy with resolution of symptoms and decrease in the size of empyema and abscess on follow-up imaging. We also include a review if literature of related fusobacterial infections.
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DZINK, J. L., M. T. SHEENAN, and S. S. SOCRANSKY. "Proposal of Three Subspecies of Fusobacterium nucleatum Knorr 1922: Fusobacterium nucleatum subsp. nucleatum subsp. nov., comb. nov.; Fusobacterium nucleatum subsp. polymorphum subsp. nov., nom. rev., comb. nov.; and Fusobacterium nucleatum subsp. vincentii subsp. nov., nom. rev., comb. nov." International Journal of Systematic Bacteriology 40, no. 1 (1990): 74–78. http://dx.doi.org/10.1099/00207713-40-1-74.
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Jabra-Rizk, Mary Ann, William A. Falkler, William G. Merz, Jacqueline I. Kelley, A. A. M. A. Baqui, and Timothy F. Meiller. "Coaggregation of Candida dubliniensiswith Fusobacterium nucleatum." Journal of Clinical Microbiology 37, no. 5 (1999): 1464–68. http://dx.doi.org/10.1128/jcm.37.5.1464-1468.1999.
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The binding of microorganisms to each other and oral surfaces contributes to the progression of microbial infections in the oral cavity. Candida dubliniensis, a newly characterized species, has been identified in human immunodeficiency virus-seropositive patients and other immunocompromised individuals.C. dubliniensis phenotypically resembles Candida albicans in many respects yet can be identified and differentiated as a unique Candida species by phenotypic and genetic profiles. The purpose of this study was to determine oral coaggregation (CoAg) partners of C. dubliniensis and to compare these findings with CoAg of C. albicansunder the same environmental conditions. Fifteen isolates ofC. dubliniensis and 40 isolates of C. albicans were tested for their ability to coaggregate with strains of Fusobacterium nucleatum,Peptostreptococcus micros, Peptostreptococcus magnus, Peptostreptococcus anaerobius,Porphyromonas gingivalis, and Prevotella intermedia. When C. dubliniensis andC. albicans strains were grown at 37°C on Sabouraud dextrose agar, only C. dubliniensis strains coaggregated with F. nucleatum ATCC 49256 and noC. albicans strains showed CoAg. However, when theC. dubliniensis and C. albicansstrains were grown at 25 or 45°C, both C. dubliniensis and C. albicans strains demonstrated CoAg with F. nucleatum. Heating theC. albicans strains (grown at 37°C) at 85°C for 30 min or treating them with dithiothreitol allowed the C. albicans strains grown at 37°C to coaggregate withF. nucleatum. CoAg at all growth temperatures was inhibited by mannose and α-methyl mannoside but not by EDTA or arginine. The CoAg reaction between F. nucleatum and the Candida species involved a heat-labile component onF. nucleatum and a mannan-containing heat-stable receptor on the Candida species. The CoAg reactions betweenF. nucleatum and the Candida species may be important in the colonization of the yeast in the oral cavity, and the CoAg of C. dubliniensis by F. nucleatum when grown at 37°C provides a rapid, specific, and inexpensive means to differentiate C. dubliniensisfrom C. albicans isolates in the clinical laboratory.
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Lee, Se Ju, Yae Jee Baek, Jin Nam Kim, et al. "Increasing Fusobacterium infections with Fusobacterium varium, an emerging pathogen." PLOS ONE 17, no. 4 (2022): e0266610. http://dx.doi.org/10.1371/journal.pone.0266610.
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Infections caused by Fusobacterium species are rare; however serious infections with complications or mortality may occur occasionally. We conducted a retrospective study to investigate the clinical features of patients with Fusobacterium infections and the differences between infections caused by the species F. necrophorum, F. nucleatum, and F. varium. Additionally, we attempted to identify risk factors for Fusobacterium-associated mortality. This study included all patients at a large tertiary care teaching hospital in South Korea with Fusobacterium infections from January 2006 to April 2021. Demographic, clinical, laboratory, and outcome data were analyzed. Multiple logistic regression analysis was performed to assess the risk factors for in-hospital mortality associated with F. nucleatum and F. varium infections. We identified 272 patients with Fusobacterium infections during the study period. The number of Fusobacterium cases has increased recently, with F. varium infections markedly increasing since 2016 and causing a significant proportion of infections. Patients with F. varium infections were older and had a higher proportion of nosocomial infections than the other groups. The F. nucleatum and F. varium groups showed higher in-hospital mortality than the F. necrophorum group. Through logistic regression analysis, APACHE II score and serum albumin level were considered risk factors for in-hospital mortality. APACHE II score was positively correlated with age, red cell distribution width, and serum blood urea nitrogen, and negatively correlated with serum albumin level. Infections caused by Fusobacterium species are increasing. F. varium causes a significant proportion of severe infections.
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Torres Mantilla, José Diego. "Comparación del efecto antibacteriano de un extracto etanólico de propóleo a dos concentraciones y del paramonoclorofenol alcanforado frente a Enterococcus faecalis y Fusobacterium nucleatum." Revista Científica Odontológica 7, no. 1 (2019): 53–65. http://dx.doi.org/10.21142/2523-2754-0701-2019-53-65.
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Objetivo: Comparar el efecto antibacteriano in vitro de un extracto etanólico de propoleo a dos concentraciones frente a Enterococcus faecalis ATCC: 29212 y Fusobacterium nucleatum ATCC: 25586 con el paramonoclorofenol alcanforado (PMCFA). Materiales y método: Se incluyeron dos grupos de 15 placas Petri con cepas activadas de Enterococcus faecalis y Fusobacterium nucleatum. Se elaboró un extracto etanólico a partir de propoleo (EEP), proveniente de la provincia de Oxapampa (Perú), y se diluyo a concentraciones del 20% y el 30%. Se comparó su efecto antibacteriano frente al PMCFA, usando clorhexidina al 2% como control positivo y agua destilada como control negativo; mediante el método de Kirby-Bauer, en un periodo de 7 días para Fusobacterium nucleatum y 24 y 48 horas para Enterococcus faecalis. Se realizó el análisis estadístico mediante el programa SPSS versión 21. Resultados: Frente a Enterococcus faecalis se obtuvieron halos de 10,32 mm, 14,23 mm y 9,10 mm a las 24 horas y halos de 11 mm, 14,96 mm y 8,94 mm a las 48 horas, para las concentraciones de EEP al 20%, el 30% y el PMCFA, respectivamente. Por su parte, frente a Fusobacterium nucleatum, halos de 18,89 mm, 23,17 mm y 13,50 mm para las concentraciones al 20%, el 30% y el PMCFA, respectivamente. Conclusiones: El extracto etanólico elaborado a partir de propoleo de Oxapampa mostro efecto antibacteriano a una concentración del 20% y el 30%, que fue significativamente mayor al del PMCFA, frente a cepas activadas de Enterococcus faecalis y Fusobacterium nucleatum.
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Amalia, Martina, Vivi Oktavia Manik, Indrawati Jafar, and Shaskhia Angelina Br Ginting. "MIC and MBC of red fruit extract (Pandanus conoideus Lam) against periodontal pathogens bacteria." Majalah Kedokteran Gigi Indonesia 8, no. 1 (2022): 69. http://dx.doi.org/10.22146/majkedgiind.65352.
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There are only few studies on the antibacterial activity of red fruit extract (Pandanus conoideus Lam) against oral pathogenic bacteria. Thus, this study aims to determine the effectiveness of red fruit extracts by looking at the Minimum inhibitory Concentrations (MIC) and Minimal Bactericidal Concentrations (MBC) against periodontal pathogenic bacteria. The subjects of this study were Streptococcus mutans (ATCC 25175), Fusobacterium nucleatum (ATCC 25586), and Porphyromonas gingivalis (ATCC 33277). The antibacterial effectiveness of red fruit extract was tested by the liquid dilution method (microdilution). The data were analyzed using the one-way ANOVA test followed by a double comparison test with the Post Hoc Least Significance Different (LSD) test method. The red fruit extract effectively inhibited and eliminated test bacteria (p <0.05). Our study showed that the red fruit extracts at a concentration of 20% could inhibit the growth of Streptococcus mutans and Porphyromonas gingivalis, which was determined as the MIC strength of 80% as MBC of both bacteria tested. Furthermore, red fruit extract at the concentration of 10% showed an inhibitory effect on the growth of Fusobacterium nucleatum, which was determined as MIC of Fusobacterium nucleatum and the strength of 40% as MBC of Fusobacterium nucleatum. The red fruit extracts were significantly effective against the growth of Streptococcus mutans, Fusobacterium nucleatum, and Porphyromonas gingivalis provide essential information for further in vivo clinical studies to determine the exact dosage and clinical effectiveness of periodontal disease.
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Maulina, Syarifah Aulia, Abdul Gani Soulissa, and Armelia Sari Widyarman. "Antibiofilm Effect of Rambutan Leaf Extract (Nephelium lappaceum L.) on Selected Periodontal Pathogens." Journal of Indonesian Dental Association 5, no. 2 (2023): 57. http://dx.doi.org/10.32793/jida.v5i2.924.
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 Introduction: In Indonesia, periodontal disease is one of the dental and oral diseases with the highest prevalence. Bacteria in subgingival plaque can cause periodontal disease, such as Porphyromonas gingivalis and Fusobacterium nucleatum. Herbal medicine has potential as an alternative in the treatment of periodontal disease, for example, rambutan leaves (Nephelium lappaceum L.) are known to have antibacterial properties because they contain flavonoids, tannins, and saponins. Objective: To determine the effects of rambutan leaf extract (Nephelium lappaceum L.) on development of Porphyromonas gingivalis and Fusobacterium nucleatum biofilms. Methods: This study was carried out using the biofilm assay method with crystal violet staining and rambutan leaf extract (Nephelium lappaceum L.) with concentrations of 100%, 50%, 25%, 12.5%, 6.25%, and 3.125% were used as the test material. A microplate reader with a wavelength of 490 nm was used to measure the biofilm density of Porpyromonas gingivalis ATCC 33277 and Fusobacterium nucleatum ATCC 25586. The data obtained were then analyzed statistically using one-way analysis of variance (ANOVA), with a significance level of p<0.05. Results: The most effective concentration of rambutan leaf extract for inhibiting biofilm development at 24 hours incubation was a 100% concentration with an average optical density (OD) of 0.147 against Porphyromonas gingivalis and a 100% concentration with an average OD of 0.077 against Fusobacterium nucleatum. Conclusion: Rambutan leaf extract (Nephelium lappaceum L.) is capable of inhibiting the development of Porphyromonas gingivalis and Fusobacterium nucleatum biofilms, respectively.
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Han, Xiang Y., Jeffrey S. Weinberg, Sujit S. Prabhu, et al. "Fusobacterial brain abscess: a review of five cases and an analysis of possible pathogenesis." Journal of Neurosurgery 99, no. 4 (2003): 693–700. http://dx.doi.org/10.3171/jns.2003.99.4.0693.
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Object. The cases of five patients with fusobacterial brain abscess are presented. The authors discuss their attempt to determine the pathogenesis. Methods. The clinical and microbiological features of five cases of fusobacterial brain abscess are reviewed. Isolates of 2031 Fusobacterium spp. and other anaerobes collected (1989–2002) at our institution were analyzed and compared for incidences and isolation sources. The findings were correlated with extensive literature on the subject. The five patients were men between 45 and 74 years of age. All experienced an insidious onset of the disease and probable hematogenous seeding of the organism(s). One patient had a monomicrobic Fusobacterium necrophorum abscess, whereas the others had polymicrobic F. nucleatum abscesses. Despite surgery and a regimen of antibiotic medications and dexamethasone, three patients experienced a paradoxical deterioration 3 days postoperatively that necessitated reevacuation of the lesion. The evacuants observed at that time contained numerous leukocytes but no microorganisms, suggesting intensified inflammation as the likely cause of deterioration. This explanation is supported by literature that fusobacteria strongly activate neutrophils. An analysis of the 2031 anaerobes from blood, wounds, and abscesses showed the considerable virulence of Fusobacterium spp., which were able to enter and/or sustain themselves in the blood circulation. This pattern was similar to that of Clostridium spp., but different from those of Peptostreptococcus spp., Bacteroides spp., and Prevotella spp., which were less invasive but more abundant. Conclusions. Some fusobacterial brain abscesses may be associated with a paradoxical postoperative deterioration, which is probably due to intensified inflammation following treatment. The blood-borne dissemination and invasive behavior of fusobacteria likely initiate such a brain abscess, and further seeding of other synergic bacteria leads to a polymicrobic abscess.
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Kaplan, C. W., R. Lux, T. Huynh, A. Jewett, W. Shi, and S. Kinder Haake. "Fusobacterium nucleatum Apoptosis-inducing Outer Membrane Protein." Journal of Dental Research 84, no. 8 (2005): 700–704. http://dx.doi.org/10.1177/154405910508400803.
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The periodontal pathogen Fusobacterium nucleatum induces apoptosis in lymphocytes. We previously identified the autotransporter protein Fap2 in F. nucleatum strain PK1594 that induced apoptosis in lymphocytes when expressed in Escherichia coli. In this study, we identified protein homologs of Fap2 in the transformable F. nucleatum strain ATCC 23726, to determine their role in the induction of apoptosis in lymphocytes. We used a new gene-inactivation vector conferring thiamphenicol resistance (pHS31) to construct a mutant deficient in one of the homologs, aim1. Transcriptional analyses demonstrated disruption of aim1 expression, and phenotypic analyses revealed a 41% decrease in the ability of the mutant to induce apoptosis in Jurkat cells, as compared with the parental strain. These studies demonstrate, in the native host cell background, the contribution of aim1 to F. nucleatum induction of apoptosis and, to the best of our knowledge, represent the first report of a genetically defined and phenotypically characterized mutation in F. nucleatum.
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Garcia-Carretero, Rafael. "Bacteraemia and multiple liver abscesses due to Fusobacterium nucleatum in a patient with oropharyngeal malignancy." BMJ Case Reports 12, no. 1 (2019): e228237. http://dx.doi.org/10.1136/bcr-2018-228237.
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Fusobacterium infections can have a wide clinical spectrum, ranging from mild infections to severe sepsis and abscess formation. This range depends partly on the patient’s underlying conditions, such as immunosuppression or malignancy. Fusobacteria are commensal rods in the oropharyngeal cavity and digestive tract, but should mucosal barrier disruption occur, in the presence of the above-mentioned predisposing conditions, fusobacteria can spread and cause infections in the soft tissues, liver and so on. An elderly woman was admitted with an altered level of consciousness (lethargy). The ensuing workup revealed a posterior oral cavity tumour (squamous cell carcinoma), Fusobacterium nucleatum bacteraemia and liver abscesses. Due to the severe sepsis, the patient was referred to our intensive care unit, but she passed away despite antibiotic treatment.
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Valeri, Clarissa, and Ciptadhi Tri Oka Binartha. "Efek antibakteri dan antibiofilm minyak atsiri cendana india (santalum album l.) Terdahap fusobacterium nucleatum dan treponema denticola (in vitro)." Jurnal Kedokteran Gigi Terpadu 6, no. 1 (2024): 93–96. http://dx.doi.org/10.25105/jkgt.v6i1.20915.
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Background: Periodontitis caused by pathogenic bacteria such as Fusobacterium nucleatum and Treponema denticola. Periodontitis prevented by chemical plaque control using chlorhexidine, but excessive chlorhexidine causes discoloration of the teeth. Indian sandalwood essential oil extract is a natural ingredient can be used as an alternative and have function inhibiting bacterial growth. Purpose: To determine the effect of Indian sandalwood essential oil in inhibiting the growth and biofilm of Fusobacterium nucleatum and Treponema denticola. Method: Fusobacterium nucleatum and Treponema denticola bacteria cultured at 37C for 24 hours. The 100% essential oil dissolved into concentrations 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56%. Antimicrobial tests using microdilution method by calculating average colonies. Biofilm test using biofilm assay method with incubation period 1, 3, and 24 hours. Biofilm assay results viewed with a microplate reader with a wavelength 490 nm. Results: Microdilution of F. nucleatum and T. denticola obtained minimal inhibition at 50% concentration. Fusobacterium nucleatum biofilm test obtained inhibition at 50% concentration for 1 hour incubation period, while it can be killed at 50% concentration for 3 hours incubation and at 50%, 25%, 12,5%, 6,25% concentration for 24 hours incubation. Treponema denticola biofilm test obtained inhibition at 50% concentration for 1 and 3 hours incubation, while it can be killed at 50%, 25%, 12,5%, 6,25% concentration for 24 hours incubation. Conclusion: Indian sandalwood essential oil extract proved effective in inhibiting the growth and biofilm of F. nucleatum and T. denticola.
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Coppenhagen-Glazer, S., A. Sol, J. Abed, et al. "Fap2 of Fusobacterium nucleatum Is a Galactose-Inhibitable Adhesin Involved in Coaggregation, Cell Adhesion, and Preterm Birth." Infection and Immunity 83, no. 3 (2015): 1104–13. http://dx.doi.org/10.1128/iai.02838-14.
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Fusobacterium nucleatumis a common oral anaerobe involved in periodontitis that is known to translocate and cause intrauterine infections. In the oral environment,F. nucleatumadheres to a large diversity of species, facilitating their colonization and creating biological bridges that stabilize the multispecies dental biofilm. Many of these interactions (called coadherences or coaggregations) are galactose sensitive. Galactose-sensitive interactions are also involved in the binding ofF. nucleatumto host cells. Hemagglutination of someF. nucleatumstrains is also galactose sensitive, suggesting that a single galactose-sensitive adhesin might mediate the interaction of fusobacteria with many partners and targets. In order to identify the fusobacterial galactose-sensitive adhesin, a system for transposon mutagenesis in fusobacteria was created. The mutant library was screened for hemagglutination deficiency, and three clones were isolated. All three clones were found to harbor the transposon in the gene coding for the Fap2 outer membrane autotransporter. The threefap2mutants failed to show galactose-inhibitable coaggregation withPorphyromonas gingivalisand were defective in cell binding. Afap2mutant also showed a 2-log reduction in murine placental colonization compared to that of the wild type. Our results suggest that Fap2 is a galactose-sensitive hemagglutinin and adhesin that is likely to play a role in the virulence of fusobacteria.
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Nardelli, Carmela, Ilaria Granata, Marcella Nunziato, et al. "16S rRNA of Mucosal Colon Microbiome and CCL2 Circulating Levels Are Potential Biomarkers in Colorectal Cancer." International Journal of Molecular Sciences 22, no. 19 (2021): 10747. http://dx.doi.org/10.3390/ijms221910747.
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Colorectal cancer (CRC) is one of the most common malignancies in the Western world and intestinal dysbiosis might contribute to its pathogenesis. The mucosal colon microbiome and C-C motif chemokine 2 (CCL2) were investigated in 20 healthy controls (HC) and 20 CRC patients using 16S rRNA sequencing and immunoluminescent assay, respectively. A total of 10 HC subjects were classified as overweight/obese (OW/OB_HC) and 10 subjects were normal weight (NW_HC); 15 CRC patients were classified as OW/OB_CRC and 5 patients were NW_CRC. Results: Fusobacterium nucleatum and Escherichia coli were more abundant in OW/OB_HC than in NW_HC microbiomes. Globally, Streptococcus intermedius, Gemella haemolysans, Fusobacterium nucleatum, Bacteroides fragilis and Escherichia coli were significantly increased in CRC patient tumor/lesioned tissue (CRC_LT) and CRC patient unlesioned tissue (CRC_ULT) microbiomes compared to HC microbiomes. CCL2 circulating levels were associated with tumor presence and with the abundance of Fusobacterium nucleatum, Bacteroides fragilis and Gemella haemolysans. Our data suggest that mucosal colon dysbiosis might contribute to CRC pathogenesis by inducing inflammation. Notably, Fusobacterium nucleatum, which was more abundant in the OW/OB_HC than in the NW_HC microbiomes, might represent a putative link between obesity and increased CRC risk.
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Octaviani, Nia Pramais, Latief Mooduto, and Achmad Sudirman. "Antibacterial potency of mangosteen pericarp extracts (Garcinia mangostana L.) against Fusobacterium nucleatum." Conservative Dentistry Journal 10, no. 2 (2020): 44. http://dx.doi.org/10.20473/cdj.v10i2.2020.44-47.
Full textAbstract:
Background: Fusobacterium nucleatum is a common bacterial in root canal with pulp necrosis and periradicular lesion. A way to eliminate these bacteria from root canal is by root canal irrigation. Root canal irrigation materials that are widely used nowadays has many shortcomings. The pericarp of mangosteen (Garcinia mangostana L) has antibacterial potency. Therefore mangosteen pericarp can be an alternative material which could inhibit and bactericidal function to Fusobacterium nucleatum. Purpose: The aim of this study was to determine the antibacterial potency of mangosteen pericarp extract (Garcinia Mangostana L.) against Fusobacterium nucleatum. Methods: This study was laboratory experimental with pos-test only control group design. A microdilution method was used to determine minimum inhibitory concentration and minimum bactericidal concentration by colony counting bacteriae in Tryptone Soya Agar (TSA) media with drop plate technique. Growth of bacterial colonies in TSA is calculated manually in colony forming unit (CFU/ml). Results: Bacterial colonies growth at concentration 0.78% was 90% less than positive control group and there were no bacterial colonies growth at concentration 0.975%. Conclusion: The Minimum Inhibitory Concentration (MIC) of mangosteen pericarp against Fusobacterium nucleatum was at 0,78% concentration and the Minimum Bactericidal Concentration (MBC) was at 0.975% concentration.
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Lee, H. R., I. C. Rhyu, H. D. Kim, et al. "In-vivo-induced antigenic determinants of Fusobacterium nucleatum subsp. nucleatum." Molecular Oral Microbiology 26, no. 2 (2011): 164–72. http://dx.doi.org/10.1111/j.2041-1014.2010.00594.x.
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Widjiastuti, Ira, S. Sukaton, Agnes Melinda Wong, and Nanik Zubaidah. "Antibacterial power effectiveness of calcium hydroxide and propolis mixture on Fusobacterium nucleatum bacteria." Conservative Dentistry Journal 9, no. 1 (2019): 1. http://dx.doi.org/10.20473/cdj.v9i1.2019.1-4.
Full textAbstract:
Background: Calcium hydroxide is a root canal dressing material that is widely used in dentistry because of its ability to regenerate hard tissue and eliminate bacteria. According to Ferreira et al. (2015), Fusobacterium nucleatum was found in 90% of teeth that had a root canal treatment done using calcium hydroxide as an intracanal medication. Due to this shortcoming of the antibacterial power of calcium hydroxide, additional research on alternative ingredients that can be combined with calcium hydroxide to improve its antibacterial power is necessary. Propolis is a natural material that has high antibacterial power and has long been used in dentistry. The addition of propolis to calcium hydroxide is expected to improve the antibacterial power of calcium hydroxide without eliminating its function in terms of regenerating hard tissue. Purpose: To find out how effective is the antibacterial power of a combination of calcium hydroxide and propolis against Fusobacterium nucleatum. Methods: The research was carried out using 4 treatment groups consisting of 6 samples for each group. Group 1 is given a combination of calcium hydroxide and propolis with a ratio of 1:1, group 2 with a ratio of 1:1.5, group 3 with a ratio of 1:2, and group 4 is a positive control of calcium hydroxide and sterile aquadest suspension. Each sample was put into a test tube containing BHIB and a suspension of Fusobacterium nucleatum, incubated at 37ºC for 24 hours, and vortexed for 1 minute. A total of 0.1 ml of bacterial inoculum was taken from each sample and then put into the MHA and grown for 24 hours. The number of Fusobacterium nucleatum colonies grown on MHA was calculated and expressed using the Colony Forming Unit (CFU). Results: There were fewer colonies of Fusobacterium nucleatum in the treatment group compared to the control group. Conclusion: The combination of calcium hydroxide and propolis has an effective antibacterial power against Fusobacterium nucleatum which the ratio of 1:2 is more effective than ratio of 1:1,5 and 1:1.