Relevant bibliographies by topics / G-cell

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'G-cell'

Author: Grafiati
Published: 4 June 2021
Last updated: 14 February 2022

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Journal articles on the topic "G-cell"

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Sawada, Mitsutaka, and Chris J. Dickinson. "The G Cell." Annual Review of Physiology 59, no. 1 (October 1997): 273–98. http://dx.doi.org/10.1146/annurev.physiol.59.1.273.

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Assmann, Sarah M. "Guard cell G proteins." Trends in Plant Science 1, no. 3 (March 1996): 73–74. http://dx.doi.org/10.1016/s1360-1385(96)89035-2.

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Clapham, David. "Cell Signalling.Noel G. Morgan." Quarterly Review of Biology 66, no. 1 (March 1991): 74. http://dx.doi.org/10.1086/417061.

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Amiot, L., B. Drénou, M. Onno, A. Bensussan, P. Le Bouteiller, G. Semana, B. Le Marchand, T. Lamy, and R. Fauchet. "HLA-G cell surface expression in hematopoietic cells." Human Immunology 47, no. 1-2 (April 1996): 144. http://dx.doi.org/10.1016/0198-8859(96)85476-0.

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Montagne, Kevin, Mutsuo Ogasawara, Jeonghyun Kim, Katsuko Furukawa, and Takashi Ushida. "GS1-18 HYDROSTATIC PRESSURE ACTIVATES HETEROTRIMERIC G PROTEINS IN CHONDROCYTE PROGENITOR CELLS(GS1: Cell and Tissue Biomechanics IV)." Proceedings of the Asian Pacific Conference on Biomechanics : emerging science and technology in biomechanics 2015.8 (2015): 131. http://dx.doi.org/10.1299/jsmeapbio.2015.8.131.

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Nakamura, Fumio, Mariko Kato, Kimihiko Kameyama, Toshihide Nukada, Tatsuya Haga, Hiroyuki Kato, Tadaomi Takenawa, and Ushio Kikkawa. "Characterization of GFamily G Proteins G(G), G(G), and GExpressed in the Baculovirus-Insect Cell System." Journal of Biological Chemistry 270, no. 11 (March 17, 1995): 6246–53. http://dx.doi.org/10.1074/jbc.270.11.6246.

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R., Ali, Ahmad Fauzi M.N., Mutharasu D., and Zainal Z.A. "G-7 EFFECT OF CELL OPERATING TEMPERATURE ON THE PERFORMANCE OF AN INTERMEDIATE TEMPERATURE SOLID OXIDE FUEL CELL(Session: Fuel Cell/Magnet)." Proceedings of the Asian Symposium on Materials and Processing 2006 (2006): 133. http://dx.doi.org/10.1299/jsmeasmp.2006.133.

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Sun, Aining, Yanling Wu, Shengli Xue, Wu Depei, and Weirong Chang. "Mechanism of CAG Regimen Eliminating Human T Cell Acute Lymphoblastic Leukemia Cell Line, A3." Blood 112, no. 11 (November 16, 2008): 5051. http://dx.doi.org/10.1182/blood.v112.11.5051.5051.

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Abstract Objective To explore the mechanism of CAG regimen eliminating human T cell acute lymphoblastic leukemia cell line, A3 and evaluate the role played by G-CSF/G-CSFR system in this process. Methods The expression of G-CSFR on A3 cells was detected by flow cytometric analysis. Cell cycle parameters of A3 cells treated with different concentration of G-CSF(5ng/ml A10ng/ml A15ng/ml A20ng/ml G0ng/ml as control) were examined by propidium iodide staining. The inhibition and apoptosis rates of A3 cells caused by treatment with various combination of G-CSF, cytarabine (Ara-C), and aclarubicin (ACR) after incubation for 48h were analyzed by Cell Counting Kit (CCK-8) and AnnexinV staining, respectively. After incubation for 48 hours with G-CSF and PD98059(the specific inhibitor of MEK in Ras-MAPK signaling pathway), cell cycle and cell dynamic change were examined. Results The expression frenquency of G-CSFR on A3 cells was 94.2% which was comparable to that of KG-1 cells. The proportion of A3 cells in S-phase was elevated concomitantly with the increasing G-CSF concentrations within 0–20ng/ml, highest at 15ng/ml of G-CSF. After incubation with Ara-C and G-CSF for 48 hours, the proliferation of A3 cells was inhibited more significantly than incubation with incubation with Ara-C alone (P<0.05, Ara-C 10−5M and 10−6M) by CCK-8 assay. Incubated with Ara-C, ACR, and G-CSF for 48 hours, the apoptosis of A3 cells was increased than that treated with Ara-C and ACR. With the concentration of PD98059 increased gradually, the proportion of A3 cells in S-phase and OD values of A3 cells decreased, which was less than that of control group (p<0.05). Conclusion G-CSFR was expressed on A3 cells. G-CSF/G-CSFR system had a synergetic effect on eliminating A3 cells when administrated simultaneously with chemical agents by driving G0-phase cells into S-phase. Apoptosis was one of the mechanisms of CAG regimen eliminating A3 cells. The interaction between G-CSF and G-CSFR activates a series of signaling pathways which includes Ras-MAPK. The inhibition of MAPK phosphorylation by PD98059 contributed partially to the effect of G-CSF on A3 cells.
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Kudo, Tomoya, Hideaki Kigoshi, Takashi Hagiwara, Takahisa Takino, Masatoshi Yamazaki, and Satoru Yui. "Cathepsin G, a Neutrophil Protease, Induces Compact Cell-Cell Adhesion in MCF-7 Human Breast Cancer Cells." Mediators of Inflammation 2009 (2009): 1–11. http://dx.doi.org/10.1155/2009/850940.

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Cathepsin G is a serine protease secreted by activated neutrophils that play a role in the inflammatory response. Because neutrophils are known to be invading leukocytes in various tumors, their products may influence the characteristics of tumor cells such as the growth state, motility, and the adhesiveness between cells or the extracellular matrix. Here, we demonstrate that cathepsin G induces cell-cell adhesion of MCF-7 human breast cancer cells resulting from the contact inhibition of cell movement on fibronectin but not on type IV collagen. Cathepsin G subsequently induced cell condensation, a very compact cell colony, resulting due to the increased strength of E-cadherin-mediated cell-cell adhesion. Cathepsin G action is protease activity-dependent and was inhibited by the presence of serine protease inhibitors. Cathepsin G promotes E-cadherin/catenin complex formation and Rap1 activation in MCF-7 cells, which reportedly regulates E-cadherin-based cell-cell junctions. Cathepsin G also promotes E-cadherin/protein kinase D1 (PKD1) complex formation, and Go6976, the selective PKD1 inhibitor, suppressed the cathepsin G-induced cell condensation. Our findings provide the first evidence that cathepsin G regulates E-cadherin function, suggesting that cathepsin G has a novel modulatory role against tumor cell-cell adhesion.
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Blaschke, Angela, Cornelis Weijer, and Harry MacWilliams. "Dictyostelium discoideum: Cell-type proportioning, cell-differentiation preference, cell fate, and the behavior of anterior-like cells in Hs1/Hs2 and G+/G− mixtures." Differentiation 32, no. 1 (August 1986): 1–9. http://dx.doi.org/10.1111/j.1432-0436.1986.tb00549.x.

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Dissertations / Theses on the topic "G-cell"

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Martinsson, Hanna-Stina. "Single cell analysis of checkpoints in G₁ /." Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-455-4/.

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Han, Tian. "Flow cell separation in fluctuating g-field." Thesis, Brunel University, 2015. http://bura.brunel.ac.uk/handle/2438/11105.

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Field flow fractionation of particles in rotating coiled column has been investigated in recent year. In contrast to the classical mode of field flow fractionation in narrow channels, the use of rotating coiled columns offers the possibility of large sample loading. In this thesis, the potential for new cell separation methods based on the use of flow fractionation in fluctuating g-fields generated in rotating coil columns is examined. The effects of operational conditions (flow rate and rotational speed – Chapter 3 and Chapter 5); cell properties (cell flexibility – Chapter 4); and column shapes (different inner diameters and coil geometries – Chapter 6) on the flow behaviour of a model system of red blood cells (RBCs) from different species, which differ markedly in size, shape & density, flowing in a single phase of buffered saline have been characterised. Operational Conditions: For a particular rotational speed, there was a minimum flow rate which caused all the cells to be retained in the column and a maximum flow rate at which all cells were eluted. Both the minimum and maximum flow rate were increased when a higher rotational speed was applied. Differences in the behaviour of sheep & hen RBCs have been used to develop a separation method using a continuously increasing flow gradient. This separation could be speeded up by using a step flow gradient. The effects of cell load and rotational direction on the behaviour of RBCs in the column was also studied in this thesis. Cell Properties: The minimum flow rate was found to correlate with cell diameter/cell volume of the RBCs as expected for a sedimentation related process and was partially described by a theoretic equation developed for particles by Fedotov and colleagues (Fedotov et al. 2005). However cell dependent departures from this equation were found which appear to indicate that cell specific surface properties may also be involved for cells (Chapter 3). By contrast the maximum flow rate showed no correlation with cell diameter/cell volume. An effect of cell deformability on the flow separation behaviour of the cells has been demonstrated. Chemical fixation of sheep RBCs with glutaraldehyde rendered the normally deformable RBCs rigid and non-deformable and resulted in the fixed sheep RBCs eluting significantly earlier than unfixed sheep RBCs. This difference was great enough that a mixture of deformable (unfixed) and non-deformable (fixed) sheep RBCs could be separated. Fixed cells tended to show cell aggregation, which could be reduced by the addition of surfactant. Column Geometry: An effect of column shapes on the flow separation behaviour of cells has been demonstrated showing that the optimisation of column design is an important feature of this mode of cell separation. For columns with the same cross sectional area, a “horizontal” rectangular column provided better separation than a circular column and a “vertical” rectangular column gave the least efficient separation. A possible explanation for this behaviour is suggested the thinner sedimentation layer and less secondary flow. Differences in the behaviour of various species of RBCs in the “horizontal” rectangular column have been used to study the efficiency of separation of a mixture of sheep and hen RBCs, and a mixture of rabbit and hen RBCs. This work shows similarities and differences with other reports on cell/particle separations in rotating coiled columns in single phases and also in aqueous two phases systems (ATPS) and these are discussed. Fedotov P.S., Kronrod V.A. & Kasatonova O.N. (2005). Simulation of the motion of solid particles in the carries liquid flow in a rotating coiled column. J. Anal. Chem., 60, 4, 310-316.
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Hölig, Kristina. "G-CSF in Healthy Allogeneic Stem Cell Donors." Karger, 2013. https://tud.qucosa.de/id/qucosa%3A71643.

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Mobilization of peripheral blood stem cells (PBSC) in healthy volunteers with granulocyte colony-stimulating factor (G-CSF) is currently carried out at many institutions worldwide. This report presents the experience of the Dresden center regarding donor evaluation and mobilization schedule. Data regarding efficacy, short- and long-term safety of G-CSF treatment gained from 8290 PBSC collections in healthy donors are outlined. These results are discussed against the background of the available evidence from the literature. Although established as a standard procedure, G-CSF application to allogeneic donors will always be a very delicate procedure and requires the utmost commitment of all staff involved to ensure maximum donor safety. (PBSC) donation does not require hospitalization and is generally assumed to be less physically demanding for the donor. However, application of mobilizing agents is stringently required for successful HSC mobilization. The standard substance, which is almost exclusively used in healthy donors worldwide, is recombinant human granulocyte colony-stimulating factor (rhG-CSF). Two preparations – filgrastim and lenograstim – are available and have been approved for PBSC mobilization for about 15 years in Germany. Currently, more than 20,000 healthy donors worldwide receive rhG-CSF for PBSC mobilization every year [7]. At the Dresden University Hospital, PBSC collections have been performed since 1996. In the two collection facilities associated with the university hospital, 8,290 allogeneic PBSC collections from 8,005 donors (i.e. 285 second collections) have been documented in a database up until May 2012. This paper presents the data of our own group, and summarizes the current knowledge regarding the short- and long-term effects of G-CSF treatment in healthy stem cell donors.
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Mohanty, Snigdha S. "Evaluation of the Imhoflot G-Cell for fine coal cleaning /." Available to subscribers only, 2007. http://proquest.umi.com/pqdweb?did=1400966001&sid=10&Fmt=2&clientId=1509&RQT=309&VName=PQD.

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Slessareva, Janna Eugenievna. "Molecular mechanisms of G protein-receptor coupling." Morgantown, W. Va. : [West Virginia University Libraries], 2003. http://etd.wvu.edu/templates/showETD.cfm?recnum=2907.

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Thesis (Ph. D.)--West Virginia University, 2003.
Title from document title page. Document formatted into pages; contains vi, 200 p. : ill. (some col.). Vita. Includes abstract. Includes bibliographical references.
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Ma, Hongzheng. "Molecular mechanisms of G protein-receptor coupling." Morgantown, W. Va. : [West Virginia University Libraries], 2003. http://etd.wvu.edu/templates/showETD.cfm?recnum=2978.

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Thesis (Ph. D.)--West Virginia University, 2003.
Title from document title page. Document formatted into pages; contains viii, 264 p. : ill. (some col.). Vita. Includes abstract. Includes bibliographical references.
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Sharfe, Nigel. "Investigation of G protein expression in human lymphoid cells." Thesis, University of Cambridge, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.308330.

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Mauk, Andrew W. "A new modeling protocol for G-protein coupled receptors : molecular simulation of phospholipid assemblies." Thesis, Georgia Institute of Technology, 1996. http://hdl.handle.net/1853/11033.

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Piva, Tayeme Cristina. "Fibras Gelatinosas em Eriosema (DC.) Desv. (Leguminosae) Distribuição e Caracterização Estrutural e Química /." Botucatu, 2020. http://hdl.handle.net/11449/192250.

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Orientador: Silvia Rodrigues Machado
Resumo: Fibras gelatinosas são células especializadas de esclerênquima que possuem uma camada interna altamente modificada de aparência gelatinosa e translúcida, denominada camada-G. Diversas funções são atribuídas as fibras gelatinosas sendo a principal e mais conhecida a sua relação com o lenho de tração. Porém, outras funções de cunho fisiológico e adaptativo, tais como armazenamento de água e manutenção da orientação foliar foram descritas para as fibras gelatinosas. As fibras gelatinosas são reportadas em diversas famílias de angiospermas nos mais diversos ambientes, com isso outras funções podem ser associadas a ocorrência das fibras gelatinosas nos mais diferentes ambientes. O objetivo desse estudo foi analisar as fibras gelatinosas em representantes de Eriosema (DC.) Desv. um gênero de leguminosa comumente encontrado em Cerrado, visando a acessar variações na distribuição e organização das fibras gelatinosas no eixo vegetativo aéreo e subterrâneo, caracterizar a estrutura básica e a histoquímica das paredes celulares. O estudo foi conduzido com 19 táxons coletados em diferentes áreas de Cerrado, sendo amostrados a folha, caule aéreo e órgão subterrâneo. O material foi processado de acordo com técnicas usuais em anatomia e histoquímica vegetal. Uma espécie foi considerada como modelo e foram utilizados os anticorpos LM5 e LM10 marcadores para galactanos e xilanos, respectivamente, nas paredes das fibras gelatinosas. As fibras gelatinosas ocorreram por todo o corpo vegetativo d... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Gelatinous fibers (G-fibers) are specialized sclerenchyma cells that have a highly modified, translucent, gelatinous appearance inner layer called the G-layer. Several functions are attributed to the G-fibers being the main and best known its relation with the tension wood. However, other physiological and adaptive functions, such as water storage and maintenance of leaf orientation, have been described for G-fibers. G-fibers are reported in several angiosperm families in the most diverse environments, so other functions may be associated with the occurrence of G-fibers in the most different environments. The purpose of this study was to analyze the G-fibers in representatives of Eriosema (DC.) Desv. a genus of legumes commonly found in Cerrado, aiming to access variations in the distribution and organization of G-fibers in the aerial and underground vegetative axis, to characterize the basic structure and histochemistry of cell walls. The study was conducted with 19 taxa collected in different areas of Cerrado, being sampled the leaf, aerial stem and underground organ. The material was processed according to usual techniques in plant anatomy and histochemistry. LM5 and LM10 antibodies that mark galactans and xylans, respectively, were used on the G-fibers walls. G-fibers occurred throughout the vegetative body of the studied Eriosema taxa, being extraxylary in regions with primary growth and xylary in regions with secondary growth. As for the organization, in the leaf the G-... (Complete abstract click electronic access below)
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Ghahremani, Mohammad H. "G protein specificity of dopamine D2S receptor signaling in cell growth and proliferation." Thesis, McGill University, 2000. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=36937.

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A wide range of hormonal, developmental and growth factors regulate cellular growth and proliferation events through receptors that couple to heterotrimeric G proteins. Upon activation of the receptor by its ligand the G protein is activated and subsequent dissociation of alpha subunit from betagamma dimer initiates a cascade of intracellular signaling events. Dopamine D2 receptors, short form (D2S) and long form (D2L) have been implicated in diverse physiological functions including the regulation of pituitary cell proliferation. Although in neuro-endocrine cells, the D2 receptor is traditionally known as an inhibitory receptor since it couples to "inhibitory" G proteins (Gi/Go), a stimulatory role of this receptor has been reported in many non-neuronal cell systems involving growth-related signaling pathways. In this thesis, I have investigated the role of the dopamine D2S receptor in cell growth under the hypothesis that "D2S receptor couples specifically to multiple combinations of Gi/o protein subtypes to initiate specific signaling pathways contributing to cellular proliferation".
At first I have examined the role of D2S-induced inhibition of adenylyl cyclase (AC). I have shown that D2S receptor activation inhibits cAMP production in Ltk-(LD2S) and Balb/c-3T3 (Balb-D2S) fibroblast cells. This effect is blocked by pertussis toxin, which specifically inactivates Gi/o proteins. Using a series of toxin-insensitive Galphai/o subtypes, I have identified that in LD2S AC inhibition is mediated through Galphai2 when AC is activated by forskolin and through Galphai3 when AC is stimulated by Galphas. In Balb-D2S cells, D2S inhibition of forskolin-inuduced AC is mediated through Galphai2 and Galphai3. Activation of D2S receptor increases Ca+2 mobilization in both cell lines. Unlike inhibition of AC, this action is mediated through Gbetagamma subunits. The D2S receptor induces MAPK activation and increases DNA synthesis in Balb-D2S cells, actions mediated through a combination of G protein subunits. I have shown that MAPK activation is mainly mediated though Galphai2 and Gbetagamma and DNA synthesis is only mediated through Galphai2 and Gbetagamma. Long term activation of D2S receptor in Balb-D2S cells induces cellular transformation and foci formation. I have shown that this effect is mediated through Galphai3, which suggests a different pathway from MAPK activation and DNA synthesis, initiated via specific G proteins, induces cellular transformation.
In conclusion, I have shown that activation of dopamine D2S receptor couples to multiple Gi/o protein subtypes to regulate different growth-related pathways. More over, I have reported that the D2S receptor induces cell growth and transformation in fibroblast cells and that the specificity of D2S receptor signaling in these pathways is dictated by G protein subunit and Galphai/o subtype. Take together, this thesis exhibits different levels of specificity in D2S signaling which provides novel insights for diseases involving alterations in G protein coupling.
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Books on the topic "G-cell"

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Adhesion-GPCRs structure to function. New York, N.Y: Springer Science+Business Media, 2010.

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Giraldo, Jesús, and Jean-Philippe Pin. G protein-coupled receptors: From structure to function. Cambridge: Royal Society of Chemistry, 2011.

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Iismaa, Tiina P. G protein-coupled receptors. Austin: R.G. Landes Co., 1995.

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J, Biden Trevor, and Shine John, eds. G protein-coupled receptors. New York: Springer-Verlag, 1995.

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Symposium, Esteve Foundation. Inverse agonism: Proceedings of the Esteve Foundation Symposium X : S'Agaró (Girona), Spain, 2-5 October 2002. Edited by IJzerman Adriaan P and Fundación Dr Antonio Esteve. Amsterdam: Elsevier, 2003.

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Nederkoorn, Paul H. J. Signal transduction by G protein-coupled receptors: Bioenergetics and G protein activation : proton transfer and GTP synthesis to explain the experimental findings. New York: Springer, 1997.

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Razvi, Enal S. GPCRs: The targets of today's drugs and tomorrow's blockbusters. Westborough, MA: D&MD Reports, 2002.

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Signal transduction protocols. 3rd ed. New York: Humana Press, 2011.

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Tobin, A. B. The Phospholipase C pathway: Its regulation and desenisitization. Austin, TX: R.G. Landes, 1996.

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Tao, Ya-Xiong. G protein-coupled receptors in health and disease. Amsterdam: Elsevier/Academic Press, 2009.

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Book chapters on the topic "G-cell"

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La Rosa, Stefano. "G-Cell." In Encyclopedia of Pathology, 1–2. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-319-28845-1_5189-1.

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Hosing, Chitra. "Hematopoietic Stem Cell Mobilization with G-CSF." In Stem Cell Mobilization, 37–47. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-943-3_3.

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Foina, Aislan G., Rosa M. Badia, and Javier Ramirez-Fernandez. "G-Means Improved for Cell BE Environment." In Lecture Notes in Computer Science, 54–65. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-16233-6_8.

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Chen, Jin-Gui. "Heterotrimeric G-Proteins and Cell Division in Plants." In Integrated G Proteins Signaling in Plants, 155–76. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-642-03524-1_9.

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Teichmann, Anke, Antje Schmidt, Burkhard Wiesner, Alexander Oksche, and Ralf Schülein. "Live Cell Imaging of G Protein-Coupled Receptors." In Methods in Molecular Biology, 139–69. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-909-9_7.

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Orbán, Erika, Davide Proverbio, Stefan Haberstock, Volker Dötsch, and Frank Bernhard. "Cell-Free Expression of G-Protein-Coupled Receptors." In Methods in Molecular Biology, 171–95. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-2230-7_10.

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Hoggatt, Jonathan, and Louis M. Pelus. "Hematopoietic Stem Cell Mobilization with Agents Other than G-CSF." In Stem Cell Mobilization, 49–67. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-943-3_4.

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Iismaa, Tiina P., Trevor J. Biden, and John Shine. "Cell Surface Receptors and the G Protein-Coupled Receptor Superfamily." In G Protein-Coupled Receptors, 1–63. Berlin, Heidelberg: Springer Berlin Heidelberg, 1995. http://dx.doi.org/10.1007/978-3-662-21930-0_1.

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McIntire, Erik, Kimberly Leonhard, Seth Taapken, and Anna Lisa Larson. "G-Banded Karyotyping of Human Pluripotent Stem Cell Cultures." In Methods in Molecular Biology, 251–68. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-1084-8_16.

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Balcerek, Michał, and Aleksander Weron. "Stochastic Dynamics of G-Protein-Coupled Cell-Surface Receptors." In Springer Proceedings in Mathematics & Statistics, 233–40. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-13881-7_26.

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Conference papers on the topic "G-cell"

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Li, Yanxia, Guangrong Ji, Haiyong Zheng, and Hui Cao. "Cell Image Decomposition Based on G-Space and PDE." In 2008 IEEE International Symposium on Knowledge Acquisition and Modeling Workshop (KAM 2008 Workshop). IEEE, 2008. http://dx.doi.org/10.1109/kamw.2008.4810511.

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Shiraishi, Toshihiko, and Akitoshi Nishijima. "A Study of a Mechanism of Cell Proliferation Promotion of Cultured Osteoblasts by Mechanical Vibration." In ASME 2018 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/imece2018-87364.

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This paper describes a mechanism of cell proliferation promotion of cultured osteoblasts by mechanical vibration focusing on β-catenin. 12.5 Hz and 0.5 G mechanical vibration was reported to promote the cell proliferation of cultured osteoblasts in plane culture. That is because the mechanical vibration weakens cell-cell adhesion, promotes to pile up cells, and allows cells to form multilayer structure. However, it has not been clarified why cells continue cell division after their monolayer confluent state. Here we show that mechanical vibration not only weakens cell-cell adhesion bound by β-catenin but also promotes to move β-catenin from the cytoplasm to the nuclei, where β-catenin associates with DNA-binding members of the Tcf/LEF family and other associated transcription factors including cell division. After osteoblastic cells were cultured under 12.5 Hz and 0.5 G mechanical vibration, cells were fractionated into nuclear and cytoplasmic fractions using a centrifugation method. β-catenin in each fraction was detected by a western blot experiment. The protein bands from western blot films were quantified with an image processing and analysis software, ImageJ. As a result, the vibration group gave higher expression of β-catenin in nuclear fraction than the non-vibration group just after the vibration group reached the saturated cell density. It indicates that 12.5 Hz and 0.5 G mechanical vibration may promote to move β-catenin into the nuclei and the cell division.
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McKahn, Denise A., and Whitney McMackin. "Characterizing Performance of a PEM Fuel Cell for a CMET Balloon." In ASME 2011 9th International Conference on Fuel Cell Science, Engineering and Technology collocated with ASME 2011 5th International Conference on Energy Sustainability. ASMEDC, 2011. http://dx.doi.org/10.1115/fuelcell2011-54532.

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We present the design of a multi-cell, low temperature PEM fuel cell for controlled meteorological balloons. Critical system design parameters that distinguish this application are the lack of reactant humidification and cooling due to the low power production, high required power mass-density and relatively short flight durations. The cell is supplied with a pressure regulated and dead ended anode, and flow controlled cathode at variable air stoichiometry. The cell is not heated and allowed to operate with unregulated temperature. Our prototype cell was capable of achieving power densities of 43 mW/cm2/cell or 5.4 mW/g. The cell polarization performance of large format PEM fuel cell stacks is an order of magnitude greater than for miniature PEM fuel cells. These performance discrepancies are a result of cell design, system architecture, and reactant and thermal management, indicating that there are significant gains to be made in these domains. We then present design modifications intended to enable the miniature PEM fuel cell to achieve power densities of 13 mW/g, indicating that additional performance gains must be made with improvements in operating conditions targeting achievable power densities of standard PEM fuel cells.
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Lv, Xiangmin, Guohua Hua, Chunbo He, John S. Davis, and Cheng Wang. "Abstract B82: G-protein coupled estrogen receptor (GPER) agonist G-1 inhibits growth of human granulosa cell tumor cells via blocking microtubule assembly." In Abstracts: AACR Special Conference on Advances in Ovarian Cancer Research: From Concept to Clinic; September 18-21, 2013; Miami, FL. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1078-0432.ovca13-b82.

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Wang, Di. "G-protein-coupled receptor controls steroid hormone signaling in cell membrane." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.107278.

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McKahn, Denise A., and Xizhu Zhao. "Channel Dimension Constraints for Miniature Low Humidity PEM Fuel Cells." In ASME 2012 10th International Conference on Fuel Cell Science, Engineering and Technology collocated with the ASME 2012 6th International Conference on Energy Sustainability. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/fuelcell2012-91504.

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Numerous applications exist requiring power for small loads (<5W) with minimal mass operating in extreme ambient conditions. Making progress toward reducing stack mass, we investigate the influence of flow field channel depth and endplate compression on cell performance. The best performance was found at endplate compressions of 139 psi, cathode channel depths of 0.032 in and anode channel depths of 0.032 in. The maximum power mass-density achieved with these 4.84 cm2 cells was 16.8 mW/g in a single cell stack. If deployed in a multicell stack, this same performance would translate to a power mass-density of 45.3 mW/g, nearing the performance of off-the-shelf lithium ion batteries (approximately 70 mW/g).
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Balraju, P., Yojana Janu, M. S. Roy, G. D. Sharma, P. Predeep, S. Prasanth, and A. S. Prasad. "Dye Sensitized Solar Cell Based On Pyronin G Dye and TiO[sub 2]." In THERMOPHYSICAL PROPERTIES OF MATERIALS AND DEVICES: IVth National Conference on Thermophysical Properties - NCTP'07. AIP, 2008. http://dx.doi.org/10.1063/1.2927540.

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Stoehr, Christine G., Robert Stoehr, Antonia Wenners, Arndt Hartmann, Simone Bertz, Verena Spath, Bernhard Walter, et al. "Abstract 3700: Association of the G/G-SNP309 variant in the mdm2 gene with earlier tumor onset in female renal cell carcinoma patients." In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-3700.

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Shikata, Tetsuo, Toshihiko Shiraishi, Shin Morishita, and Ryohei Takeuchi. "Effects of Acceleration Amplitude and Frequency of Mechanical Vibration on Cultured Osteoblasts." In ASME 2008 International Mechanical Engineering Congress and Exposition. ASMEDC, 2008. http://dx.doi.org/10.1115/imece2008-67221.

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This paper describes the effects of the frequency and acceleration amplitude of mechanical vibration on osteoblasts, the bone cells that generate the bone matrix. Their cell proliferation and bone matrix generation were investigated when sinusoidal inertia force was applied to the cells. Bone formation is subject in vivo to mechanical stimulation. Although many researches for bone cells of osteoblastic lineage sensing and responding to mechanical stimulation have been reported mainly in the biochemical field, effects of mechanical stimulation on bone cells are not well understood. After the cells were cultured in culture plates in a CO2 incubator for one day and adhered on the cultured plane, vibrating groups of the culture plates were set on an aluminum plate attached to a exciter and cultured under sinusoidal excitation in another incubator separated from non-vibrating groups of the culture plates. Acceleration amplitude and frequency were set to several kinds of conditions. The time evolution of cell density was obtained by counting the number of cells with a hemocytometer. Calcium salts generated by the cells were observed by being stained with alizarin red S solution and their images were captured with a CCD camera. The vibrating groups for the cell proliferation and the calcium salts staining were sinusoidally excited for 24 hours a day during 28 days of culture. Gene expression of alkaline phosphatase (ALP) and runt-related gene 2 (Runx2) was measured by a real-time reverse transcription polymerase chain reaction (real-time RT-PCR) method. After the vibrating groups for the PCR were excited for 4 days, the total RNAs were extracted. After reverse transcription, real-time RT-PCR was performed. Gene expression for ALP, Runx2, and a housekeeping gene were determined simultaneously for each sample. ALP and Runx2 gene level in each sample was normalized to the measured housekeeping gene level. The following experimental results of sinusoidal excitation of osteoblasts have been shown: (a) Cell density decreased at 0.5 G with increasing frequency in the range from 12.5 to 1000 Hz and increased at 25 Hz with increasing acceleration amplitude from 0 to 0.5 G at 14 days of culture. (b) No calcium salts were observed in the non-vibrating group and the areas of calcium salts observed in the 0.5 G vibration group were larger than those in the 0.25 G group at 25 Hz at 21 days of culture. (c) The mRNA level of ALP at 0.5 G showed the peak at 50 Hz in the range from 12.5 to 1000 Hz and that at 50 Hz showed the peak at 0.5 G in the range from 0.25 to 1 G at 4 days of culture. In the case of Runx2, the same tendency was found. It has been shown that it is important to consider mechanical vibration as well as biochemical aspects in studies of the functional adaptation of cells to mechanical stimulation.
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Agarwal, Saurabh, Zaowen Chen, Danielle Hsu, Eugene Kim, and Jason M. Shohet. "Abstract 3744: G-CSF-dependent regulation of STAT3 in neuroblastoma cancer stem cell subpopulations." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-3744.

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Reports on the topic "G-cell"

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Kinney, Kathryn A. Project Plan 7930 Cell G PaR Remote Handling System Replacement. Office of Scientific and Technical Information (OSTI), October 2009. http://dx.doi.org/10.2172/970923.

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Jones, Robert Wesley, and Kenneth Marshall Hargis. Hot Cell Liners Category of Transuranic Waste Stored Below Ground within Area G. Office of Scientific and Technical Information (OSTI), September 2014. http://dx.doi.org/10.2172/1258360.

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Goedhart, Paul W., and Hilko van der Voet. Statistical analysis of differential white blood cell counts for G-TwYST studies A and C. Wageningen: Biometris, Wageningen University & Research, 2018. http://dx.doi.org/10.18174/456855.

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Mandry, G. J., and R. W. Grisham. Decontamination Project for Cell G of the Metal Recovery Facility at Oak Ridge National Laboratory, Oak Ridge, Tennessee. Office of Scientific and Technical Information (OSTI), February 1994. http://dx.doi.org/10.2172/10140208.

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Schwartz, J. L., J. Cowan, and D. J. Grdina. Attenuation of G{sub 2} cell cycle checkpoint control in human tumor cells is associated with increased frequencies of unrejoined chromosome breaks but not increased cytotoxicity following radiation exposure. Office of Scientific and Technical Information (OSTI), August 1997. http://dx.doi.org/10.2172/510590.

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O'Malley, Patrick D. High-g shock test results of Tadiran TLM-1530MP cells. Office of Scientific and Technical Information (OSTI), June 2009. http://dx.doi.org/10.2172/970232.

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Granitzki, Richard F., and Aaron Barton. High-G Verification of Lithium-Polymer (Li-Po) Pouch Cells. Fort Belvoir, VA: Defense Technical Information Center, May 2016. http://dx.doi.org/10.21236/ad1009209.

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None, None. Appendix G - GPRA06 hydrogen, fuel cells, and infrastructure technologies (HFCIT) program. Office of Scientific and Technical Information (OSTI), January 2009. http://dx.doi.org/10.2172/1216501.

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Author, Not Given. Appendix G: GPRA05 Hydrogen, Fuel Cells, and Infrastructure Technologies program documentation. Office of Scientific and Technical Information (OSTI), January 2009. http://dx.doi.org/10.2172/1216650.

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Miller, Donald M., Paula J. Bates, and John O. Trent. Unique G-Rich Oligonucleotides Which Inhibit the Growth of Prostatic Carcinoma Cells. Fort Belvoir, VA: Defense Technical Information Center, July 2001. http://dx.doi.org/10.21236/ada407225.

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