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Journal articles on the topic 'Galleria mellonella larvae'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

S, Amizhthini, Yasodha P, and P. Anandhi. "Mass Rearing and Biological Characteristics of Galleria mellonella L. (Lepidoptera: Pyralidae) Using an Artificial Diet." Journal of Advances in Biology & Biotechnology 27, no. 12 (2024): 1011–16. https://doi.org/10.9734/jabb/2024/v27i121847.

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Galleria mellonella L. is an important pest of honeybees. The primary objective of the study is mass-rearing Galleria mellonella using a modified artificial diet. Globally, Galleria baiting technique with larvae of the greater wax moth, G. mellonella L., is the most commonly used method for recovering entomopathogenic fungi and infective-stage juveniles of entomopathogenic nematodes from soil. Therefore G. mellonella was mass reared at laboratory condition using modified method of Birah et al. (2008) by artificial diet. The mean larval duration for the 1st, 2nd, 3rd, 4th, 5th, 6th and 7th inst

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Sarikaya, A., and A. Gülel. "Effects of host species, stage and size on the sex ratio and clutch size of the parasitoid,Dibrachys boarmiae(Walker, 1863) (Hymenoptera: Pteromalidae)." Bulletin of Entomological Research 101, no. 3 (2011): 325–31. http://dx.doi.org/10.1017/s0007485310000532.

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AbstractEffects of host species, stage and size on clutch size and sex ratio of the gregarious, idiobiont ectoparasitoidDibrachys boarmiaewere investigated at 25±2°C and 70±5% relative humidity. The greater wax moth,Galleria mellonella, small wax moth,Achroia grisella, and early stage solitary larvae of the endoparasitoid,Apanteles galleriae, were used as hosts. Clutch size was greatest from prepupae of the largest host,Galleria mellonella, with a mean of 40.07 offspring per host versus 14.73 and 2.93 forAchroia grisellaandApanteles galleriae, respectively. The mean clutch size from pupae was

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Sokolov, Vladislav, Anastasiya Osokina, and Vladimir Kasatkin. "GALLERIA MELLONELLA L. GARBAGE SORTING MECHANIZATION TECHNOLOGY." Vestnik of Kazan State Agrarian University 15, no. 2 (2020): 120–24. http://dx.doi.org/10.12737/2073-0462-2020-120-124.

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In the modern world, insects are widely used for scientific and industrial purposes. For their cultivation in the laboratory, various plants have been developed, the main disadvantage of which is poor-quality sorting from garbage. Therefore, the question of cultivating and sorting the larvae of bee moth (Galleria mellonella L.) in laboratory conditions for the purpose of further use as a model object in various fields of biological sciences and medical purposes is relevant. The effect of the temperature gradient and exposure on the movement of G. mellonella larvae from the heated compartment o

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Ono, Masaya, and Toyoshi Yoshiga. "Cellular immunity in the insect Galleria mellonella against insect non-parasitic nematodes." Parasitology 146, no. 6 (2018): 708–15. http://dx.doi.org/10.1017/s003118201800210x.

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AbstractImmunity to microbial infections is well understood; however, information regarding the immunity to parasitic multicellular organisms remains lacking. To understand innate host cellular immunity to nematodes, we compared the cellular response of the greater wax moth (Galleria mellonella) larvae against the non-parasitic, bacterial-feeding nematode Caenorhabditis elegans and pathogenic nematode Heterorhabditis bacteriophora. When intact first-instar or dauer larvae of C. elegans were injected into a G. mellonella larva, most of the nematodes were alive and not confined by the surroundin

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Das, Srinjoy, and Kaustav Chakraborty. "Report of a New Larval Host Plant of Galleria mellonella (Linnaeus, 1758) from Kolkata, West Bengal, India (Lepidoptera: Pyralidae, Galleriinae)." SHILAP Revista de lepidopterología 52, no. 206 (2024): 255–59. http://dx.doi.org/10.57065/shilap.901.

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In this study, we report for the first time a new larval host plant Cassia fistula L. for Galleria mellonella (Linnaeus, 1758) from Kolkata, West Bengal, India. Galleria mellonella is an established pest of beehives and till date, its larvae were known to feed only on the wax comb of Apis sp.,and destroy it. For this reason, beekeeping enterprises face high economic losses. Our finding opens a scope for extensive surveys in areas surrounding apiaries in order to quantify infestations in C. fistula which might provide a new insight in the pest management practices for G. mellonella.

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Sheehan, Gerard, Laura Tully, and Kevin A. Kavanagh. "Candida albicans increases the pathogenicity of Staphylococcus aureus during polymicrobial infection of Galleria mellonella larvae." Microbiology 166, no. 4 (2020): 375–85. http://dx.doi.org/10.1099/mic.0.000892.

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This study detailed the responses of Galleria mellonella larvae to disseminated infection caused by co-infection with Candida albicans and Staphylococcus aureus . Doses of C. albicans (1×105 larva−1) and S. aureus (1×104 larva−1) were non-lethal in mono-infection but when combined significantly (P<0.05) reduced larval survival at 24, 48 and 72 h relative to larvae receiving S. aureus (2×104 larva−1) alone. Co-infected larvae displayed a significantly higher density of S. aureus larva−1 compared to larvae infected solely with S. aureus . Co-infection resulted in dissemination throughout the

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Shinde, Swati P., V. K. Biradar, D. B. Ingole, N. V. Lavhe, and Pragati R. Jawake. "Productivity of the entomopathogenic nematode, Heterorhabditis indica isolates in Galleria mellonella." Ecology, Environment and Conservation 29 (2023): 431–35. http://dx.doi.org/10.53550/eec.2023.v29i02s.070.

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Heterorhabditis indica, CICR-HI-MN and CICR-HI-CL isolates were tested over the fifth larval instar of the Greater wax moth, Galleria mellonella L. (Lepidoptera: Pyralidae), at nine different dosages, i.e., at 10, 20, 40, 60, 80, 100, 120, 140, and 160 infective juveniles (IJs)/larva in 100 µl of sterile water under laboratory condition at the district Nagpur of Vidharbha Region, India, during 2022. When the LC50 and LC95 values of 5th instar larvae were analyzed, the CICR-HI-CL isolate was quite more efficient than CICR-HI-MN isolate. Moreover, when compared to various treatments, given isola

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Lalita, Kumar Y. and Yadav S. "Effectiveness of different plant extracts against Galleria mellonella larvae in laboratory." Archives of Agriculture and Environmental Science 3, no. 1 (2018): 64–67. https://doi.org/10.26832/24566632.2018.030109.

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The present investigation was undertaken to study the effectiveness of different plant extracts against <em>Galleria mellonella </em>larvae in laboratory. Acetone prepared extracts of leaves, stem, seed, root, husk of thirteen medicinal and healthy plants were used to examine their effects on the mortality of the greater wax moth <em>Galleria mellonella </em>larvae in laboratory.  Results revealed that only six plant extracts prepared with leaves and stem cause mortality of wax moth. Larval mortality was highest (93.33%) with husk prepared extract of<em> P. psylli

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Sankar, M., J. Prasad, A. P. Padmakumari, G. Katti, and K. Divya. "COMBINED APPLICATION OF TWO ENTOMOPATHOGENIC NEMATODES, HETERORHABDITIS INDICA AND STEINERNEMA ASIATICUM TO CONTROL THE RICE LEAF FOLDER, CNAPHALOCROSIS MEDINALIS (GOEN.)." Journal of Biopesticides 02, no. 02 (2009): 135–40. http://dx.doi.org/10.57182/jbiopestic.2.2.135-140.

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ABSTRACT In this study, the efficacy of two entomopathogenic nematode species, Heterorhabditis indica and Steinernema asiaticum were evaluated against the larvae of the rice leaf folder, Cnaphalocrosis medinalis and the greater wax moth, Galleria mellonella. Combined inoculation of H. indica and S. asiaticum each at 75 IJs/larva resulted in faster larval mortality on C. medinalis (24.6 h) and G. mellonella (31 h). The percent larval mortality caused by H. indica alone was resulted significantly more (90% on G. mellonella and 60% on C. medinalis) than S. asiaticum (10% on G. mellonella and 40%

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Piatek, Magdalena, Gerard Sheehan, and Kevin Kavanagh. "Galleria mellonella: The Versatile Host for Drug Discovery, In Vivo Toxicity Testing and Characterising Host-Pathogen Interactions." Antibiotics 10, no. 12 (2021): 1545. http://dx.doi.org/10.3390/antibiotics10121545.

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Larvae of the greater wax moth, Galleria mellonella, are a convenient in vivo model for assessing the activity and toxicity of antimicrobial agents and for studying the immune response to pathogens and provide results similar to those from mammals. G. mellonella larvae are now widely used in academia and industry and their use can assist in the identification and evaluation of novel antimicrobial agents. Galleria larvae are inexpensive to purchase and house, easy to inoculate, generate results within 24–48 h and their use is not restricted by legal or ethical considerations. This review will h

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Aamer, Neama A., Zeinab A. El-Moaty, Maria Augustyniak, Lamia M. El-Samad, and Hanaa S. Hussein. "Impacts of Combining Steinernema carpocapsae and Bracon hebetor Parasitism on Galleria mellonella Larvae." Insects 15, no. 8 (2024): 588. http://dx.doi.org/10.3390/insects15080588.

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The greater wax moth, Galleria mellonella, is a significant pest in apiculture and a well-established model organism for immunological and ecotoxicological studies. This investigation explores the individual and combined effects of the ectoparasite Bracon hebetor (B.h.) and the entomopathogenic nematode Steinernema carpocapsae (S.c.) on G. mellonella larvae. We evaluated the activity of oxidative stress enzymes, including superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione S-transferase (GST), malondialdehyde (MDA) levels, cytochrome P450 activity, cell viability using Annexi

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Dunphy, Gary B., Ursula Oberholzer, Malcolm Whiteway, Robert J. Zakarian, and Iian Boomer. "Virulence of Candida albicans mutants toward larval Galleria mellonella (Insecta, Lepidoptera, Galleridae)." Canadian Journal of Microbiology 49, no. 8 (2003): 514–24. http://dx.doi.org/10.1139/w03-064.

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Culture medium affected the virulence of a strain of Candida albicans toward Galleria mellonella larvae, but the yeast growth rates in yeast extract peptone dextrose broth and synthetic Galleria serum were not correlated with yeast virulence. Virulent C. albicans grew rapidly in larval serum, whereas, it limited nodulation and continued development in vivo, producing toxins that damaged the hemocytes and fat body. Nonpathogenic yeast-phase cells grew slowly in larval serum but induced extensively melanized nodules in vivo and developed no further. There was no discernible relationship in 1

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Cé, Rodrigo, Rodrigo Campos Silva, Danielle Silva Trentin, et al. "Galleria mellonella Larvae as an In Vivo Model to Evaluate the Toxicity of Polymeric Nanocapsules." Journal of Nanoscience and Nanotechnology 20, no. 3 (2020): 1486–94. http://dx.doi.org/10.1166/jnn.2020.17170.

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Galleria mellonella larvae is an invertebrate that has been extensively used as experimental model in the investigation of microbial virulence and efficacy of antimicrobial agents and can be used to provide faster and cheaper data than traditional test systems. Our objective was to propose the use of G. mellonella larvae as an In Vivo model to evaluate the toxicity of lipid-core nanocapsule (LNC) formulations having different surface coatings. Blank LNC formulations were coated with polysorbate 80 (LNC-1), lecithin and polysorbate 80 (LNC-2), and lecithin, chitosan and polysorbate 80 (LNC-3).

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Hasan, Yeasmin, Athanassiou, Bari, and Islam. "Using Gamma Irradiated Galleria mellonella L. and Plodia interpunctella (Hübner) Larvae to Optimize Mass Rearing of Parasitoid Habrobracon hebetor (Say) (Hymenoptera: Braconidae)." Insects 10, no. 8 (2019): 223. http://dx.doi.org/10.3390/insects10080223.

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We evaluated possible improvements to the mass rearing of the larval parasitoid Habrobracon hebetor (Say) (Hymenoptera: Braconidae) on irradiated host wax moth Galleria mellonella L. and Indian meal moth Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae) larvae. The use of irradiated G. mellonella and P. interpunctella larvae at the dose of 150 Gy proved useful for enhancing the parasitism and adult emergence of H. hebetor due to the absence of negative repercussions on parasitoid development. Overall, parasitism was increased as the host larvae was irradiated with higher doses, while sig

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Roh, Seung Jin, Haechul Park, Seong-Hyun Kim, So-Yun Kim, Yong-Su Choi, and Jeong-Hun Song. "A new species of Galleria Fabricius (Lepidoptera, Pyralidae) from Korea based on molecular and morphological characters." ZooKeys 970 (September 21, 2020): 51–61. http://dx.doi.org/10.3897/zookeys.970.54960.

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The greater wax moth, Galleria mellonella Linnaeus, is well known as a pest of honey bees and for the biodegradation of wax and polyethylene by their larvae. The genus Galleria has long been considered monotypic and found worldwide. A taxonomic study of the genus Galleria is presented based on morphological and molecular characters (COI, CAD, wg). A new species (Galleria similis Roh &amp; Song, sp. nov.) is recognized on the Korean peninsula. The new species is superficially similar to G. mellonella but they can be separated by the structures of hindwing venation and male genitalia. Habitu

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Roh, Seung Jin, Haechul Park, Seong-Hyun Kim, So-Yun Kim, Yong-Su Choi, and Jeong-Hun Song. "A new species of Galleria Fabricius (Lepidoptera, Pyralidae) from Korea based on molecular and morphological characters." ZooKeys 970 (September 21, 2020): 51–61. https://doi.org/10.3897/zookeys.970.54960.

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The greater wax moth, Galleria mellonella Linnaeus, is well known as a pest of honey bees and for the biodegradation of wax and polyethylene by their larvae. The genus Galleria has long been considered monotypic and found worldwide. A taxonomic study of the genus Galleria is presented based on morphological and molecular characters (COI, CAD, wg). A new species (Galleria similis Roh & Song, sp. nov.) is recognized on the Korean peninsula. The new species is superficially similar to G. mellonella but they can be separated by the structures of hindwing venation and male genitalia. Habitus ph

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Wojda, Iwona, and Patryk Kowalski. "Galleria mellonella infected with Bacillus thuringiensis involves Hsp90." Open Life Sciences 8, no. 6 (2013): 561–69. http://dx.doi.org/10.2478/s11535-013-0162-9.

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AbstractInsects are good models for studying the innate immune response. We report that Galleria mellonella larvae infected with entomopathogenic bacteria Bacillus thuringiensis kurstaki show changes in the level of Hsp90. Our experimental approach was to pre-treat larvae with the Hsp90-binding compound, 17-DMAG, before infection with B. thuringiensis. We show that pre-treated animals display a higher level of immune response. This was mainly manifested by enhanced action of their hemolymph directed toward living bacteria as well as lysozyme activity digesting bacterial peptidoglycan. The obse

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Shalaw Abdalrahman Omer, Ibrahim Majeed Faraj, and Nask Muhamad Faraj. "Insecticidal Activity of Some Plant Extract Against Greater Wax Moth Larvae (Galleria mellonella L.)." Agricultural Science 6, no. 2 (2023): 78–88. http://dx.doi.org/10.55173/agriscience.v6i2.88.

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Galleria mellonella L. (Lepidoptera: Pyralidae) is a significant international pest and the most devastating pest throughout the world of beeswax. Natural pesticides must be replaced with synthetic materials since they are more ecologically friendly. This study aims to determine alternative methods of controlling the larger wax moth by assessing the efficacy of several plant-based biocides for this purpose various ethanolic plant extracts such as rosemary (Rosmarinus officinalis L), Clove (Syzygium aromaticum), Eucalyptus (Eucalyptus sp) and Cinnamon (Cinnamomum verum) where used with four ext

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Firacative, Carolina, Aziza Khan, Shuyao Duan, Kennio Ferreira-Paim, Diana Leemon, and Wieland Meyer. "Rearing and Maintenance of Galleria mellonella and Its Application to Study Fungal Virulence." Journal of Fungi 6, no. 3 (2020): 130. http://dx.doi.org/10.3390/jof6030130.

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Galleria mellonella larvae have been widely used as alternative non-mammalian models for the study of fungal virulence and pathogenesis. The larvae can be acquired in small volumes from worm farms, pet stores, or other independent suppliers commonly found in the United States and parts of Europe. However, in countries with no or limited commercial availability, the process of shipping these larvae can cause them stress, resulting in decreased or altered immunity. Furthermore, the conditions used to rear these larvae including diet, humidity, temperature, and maintenance procedures vary among t

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Kłudkiewicz, B., J. Godlewski, K. Grzelak, B. Cymborowski, and Z. Lassota. "Influence of low temperature on the synthesis of some Galleria mellonella proteins." Acta Biochimica Polonica 43, no. 4 (1996): 639–44. http://dx.doi.org/10.18388/abp.1996_4460.

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In developing Galleria mellonella larvae (reared at 30 degrees C) three proteins of 74, 76 and 81/82 kDa were identified. They represent a group of storage proteins (LHP proteins). In Galleria larvae, the development of which is arrested by low temperature (18 degrees C), accumulation of the 74, 76 and 81/82 kDa proteins was detected in the hemolymph. The synthesis of 74 kDa and 76 kDa proteins started after 24 h, and that of about 80 kDa after 96 h following the transfer of larvae from 30 degrees C to 18 degrees C. 20-Hydroxyecdysone inhibited synthesis of the 74 and 76 kDa proteins in larvae

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Makavchik, S. A. "Large wax moth and it’s life products for application in aquaculture and agriculture." Legal regulation in veterinary medicine, no. 2 (July 18, 2024): 102–5. http://dx.doi.org/10.52419/issn2782-6252.2024.2.102.

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This review article focuses on the biological characteristics of the wax moth (Galleria mellonella), as well as its application in aquaculture and agriculture. An analysis of the use of wax moths and their waste products as feed products for animals, fish and birds was carried out. The European Union (EU) allows the use of various plant species as feed ingredients for fish, domestic animals, birds and pigs. Due to its high content of proteins and beneficial microelements, wax moth larva can be used in food products as an alternative protein. Biomass extracts contain large amounts of amino acid

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Senior, Nicola J., and Richard W. Titball. "Isolation and primary culture of Galleria mellonella hemocytes for infection studies." F1000Research 9 (February 16, 2021): 1392. http://dx.doi.org/10.12688/f1000research.27504.2.

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Galleria mellonella larvae are increasingly used to study the mechanisms of virulence of microbial pathogens and to assess the efficacy of antimicrobials. The G. mellonella model can faithfully reproduce many aspects of microbial disease which are seen in mammals, and therefore allows a reduction in the use of mammals. The model is now being widely used by researchers in universities, research institutes and industry. An attraction of the model is the interaction between pathogen and host. Hemocytes are specialised phagocytic cells which resemble neutrophils in mammals and play a major role in

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Senior, Nicola J., and Richard W. Titball. "Isolation and primary culture of Galleria mellonella hemocytes for infection studies." F1000Research 9 (December 3, 2020): 1392. http://dx.doi.org/10.12688/f1000research.27504.1.

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Galleria mellonella larvae are increasingly used to study the mechanisms of virulence of microbial pathogens and to assess the efficacy of antimicrobials. The G. mellonella model can faithfully reproduce many aspects of microbial disease which are seen in mammals, and therefore allows a reduction in the use of mammals. The model is now being widely used by researchers in universities, research institutes and industry. An attraction of the model is the interaction between pathogen and host. Hemocytes are specialised phagocytic cells which resemble neutrophils in mammals and play a major role in

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Pereira, Elisa Dias, Thalison Rodrigues Moreira, Vanessa Rafaela Milhomem Cruz-Leite, et al. "Paracoccidioides lutzii Infects Galleria mellonella Employing Formamidase as a Virulence Factor." PLOS Neglected Tropical Diseases 18, no. 9 (2024): e0012452. http://dx.doi.org/10.1371/journal.pntd.0012452.

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The formamidase (FMD) enzyme plays an important role in fungal thriving by releasing a secondary nitrogen source as a product of its activity. In Paracoccidioides species, previous studies have demonstrated the upregulation of this enzyme in a wide range of starvation and infective-like conditions. However, Paracoccidioides lutzii formamidase has not yet been defined as a virulence factor. Here, by employing in vivo infections using an fmd-silenced strain in Galleria mellonella larvae model, we demonstrate the influence of formamidase in P. lutzii’s immune stimulation and pathogenicity. The fo

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Jegorov, Alexandr, Vladimír Maťha, and Hynek Hradec. "Detoxification of destruxins in Galleria mellonella L. larvae." Comparative Biochemistry and Physiology Part C: Comparative Pharmacology 103, no. 1 (1992): 227–29. http://dx.doi.org/10.1016/0742-8413(92)90256-7.

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Eskafi, F. M., R. E. McCoy, and R. C. Norris. "Pathology of Spiroplasma floricola in Galleria mellonella larvae." Journal of Invertebrate Pathology 49, no. 1 (1987): 1–13. http://dx.doi.org/10.1016/0022-2011(87)90119-4.

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Andrade, Mariana, Joana Neves, Maria Bento, et al. "Evaluation of Amlodipine and Imipramine Efficacy to Treat Galleria mellonella Infection by Biofilm-Producing and Antimicrobial-Resistant Staphylococcus aureus." Antibiotics 14, no. 2 (2025): 183. https://doi.org/10.3390/antibiotics14020183.

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Background/Objectives: Antimicrobial-resistant Staphylococcus aureus is a growing threat to human health for which alternative therapeutic options are needed. In this study, we aimed to evaluate the efficacy of amlodipine (AML) and imipramine (IMI) to treat S. aureus infection in the Galleria mellonella larval model by targeting efflux and biofilms, which are relevant contributors to antimicrobial resistance and virulence in S. aureus. Methods: In-house reared G. mellonella were used in virulence assays to determine the infective dose of two S. aureus strains differing in the expression of nor

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Hurst, Mark R. H., Amy K. Beattie, Sandra A. Jones, Pei-Chun Hsu, Joanne Calder, and Chikako van Koten. "Temperature-Dependent Galleria mellonella Mortality as a Result of Yersinia entomophaga Infection." Applied and Environmental Microbiology 81, no. 18 (2015): 6404–14. http://dx.doi.org/10.1128/aem.00790-15.

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ABSTRACTThe bacteriumYersinia entomophagais pathogenic to a range of insect species, with death typically occurring within 2 to 5 days of ingestion.Per oschallenge of larvae of the greater wax moth (Galleria mellonella) confirmed thatY. entomophagawas virulent when fed to larvae held at 25°C but was avirulent when fed to larvae maintained at 37°C. At 25°C, a dose of ∼4 × 107CFU per larva of aY. entomophagatoxin complex (Yen-TC) deletion derivative, theY. entomophagaΔTC variant, resulted in 27% mortality. This low level of activity was restored to near-wild-type levels by augmentation of the di

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Maekawa, Lilian Eiko, Rodnei Dennis Rossoni, Júnia Oliveira Barbosa, Antonio Olavo Cardoso Jorge, Juliana Campos Junqueira, and Marcia Carneiro Valera. "Different Extracts of Zingiber officinale Decrease Enterococcus faecalis Infection in Galleria mellonella." Brazilian Dental Journal 26, no. 2 (2015): 105–9. http://dx.doi.org/10.1590/0103-6440201300199.

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Dried, fresh and glycolic extracts of Zingiber officinale were obtained to evaluate the action against G. mellonella survival assay against Enterococcus faecalis infection. Eighty larvae were divided into: 1) E. faecalis suspension (control); 2) E. faecalis + fresh extract of Z. officinale (FEO); 3) E. faecalis + dried extract of Z. officinale (DEO); 4) E. faecalis + glycolic extract of Z. officinale (GEO); 5) Phosphate buffered saline (PBS). For control group, a 5 μL inoculum of standardized suspension (107 cells/mL) of E. faecalis (ATCC 29212) was injected into the last left proleg of each l

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Mak, P., D. Chmiel, and G. J. Gacek. "Antibacterial peptides of the moth Galleria mellonella." Acta Biochimica Polonica 48, no. 4 (2001): 1191–95. http://dx.doi.org/10.18388/abp.2001_3890.

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The work describes purification and biochemical characterization of two inducible antimicrobial peptides from the hemolymph of Galleria mellonella. The peptides were isolated by a sequence of reversed-phase chromatography steps from the hemolymph of larvae immunized with viable bacteria. The first peptide is a member of the cecropin family while the second one is rich in proline residues and has a unique sequence.

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Alves, Mariana de Sá, Luciana Ruano de Oliveira Fugisaki, Jéssica Diane dos Santos, et al. "Antifungal effects of Streptococcus mutans extract on Candida strains susceptible and resistant to fluconazole: An in vivo study." Medical Mycology 59, no. 7 (2021): 744–47. http://dx.doi.org/10.1093/mmy/myab008.

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Abstract Previous studies showed that the crude extract obtained from Streptococcus mutans inhibited the growth of Candida albicans reference strains. In this study, we evaluated whether the antifungal effects of S. mutans extract can be extended to clinical Candida isolates, including C. albicans and non-abicans strains with different susceptibilities to fluconazole. We verified that S. mutans extract increased the survival of Galleria mellonella larvae infected with C. albicans and C. glabrata and inhibited the fungal cells in hemolymph. These antifungal effects occurred for both fluconazole

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Bharathi, N. Santhana, A. Deepa, P. Chithra, A. Aasha, and K. Sujatha. "Biochemical changes in Holotrichia repetita Sharp (Coleoptera: Melolonthidae) and Galleria mellonella L. (Lepidoptera: Pyralidae) due to the biopesticide Steinernema sp." ENTOMON 44, no. 3 (2019): 197–202. http://dx.doi.org/10.33307/entomon.v44i3.461.

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In the present study, Steinernema- sp. were isolated from Tirupur soil sample and tested for its antagonistic potential against larvae of Galleria mellonella and Holotrichia repetita in laboratory condition. 100 per cent mortality was observed after 24 and 48 hours for G. mellonella and repetita respectively. Further biochemical estimations viz., protein, carbohydrates and lipids were carried out after 24 and 48 h of application. The results showed that there was a significant decrease in all biochemical parameters of parasitized larvae compared to control. Steinernema sp. can be incorporated

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KAUR, NAVDEEP, DAVINDER SINGH, AMANDEEP SINGH, KAPILA MAHAJAN, ABHINAY THAKUR, and AMANDEEP AND RANDEEP SINGH. "Efficacy of Botanical Pesticides on Biology and Cellular Immune Active Response of Greater Wax Moth, Galleria mellonella Linnaeus." BIOPESTICIDES INTERNATIONAL 19, no. 02 (2023): 199. http://dx.doi.org/10.59467/bi.2023.19.199.

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Many factors restrict honey bees? productivity and pollination potential, especially with the infestation of Greater wax moth, Galleria mellonella L., and synthetic pesticides used to manage this pest. Therefore, a study was conducted to examine the efficacy of oils extracted from Pongamia pinnata, Mentha piperita, Ocimum basilicum, and Thymus vulgaris on the biology and survival of wax moth. The results revealed that all treatments exhibited control over the hatching potential of eggs, and the lowest egg hatchability (6.67%) was observed with peppermint and thyme oil (4%). The larval mortalit

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Moya-Andérico, Laura, Joana Admella, Rodrigo Fernandes, and Eduard Torrents. "Monitoring Gene Expression during a Galleria mellonella Bacterial Infection." Microorganisms 8, no. 11 (2020): 1798. http://dx.doi.org/10.3390/microorganisms8111798.

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Galleria mellonella larvae are an alternative in vivo model that has been extensively used to study the virulence and pathogenicity of different bacteria due to its practicality and lack of ethical constraints. However, the larvae possess intrinsic autofluorescence that obstructs the use of fluorescent proteins to study bacterial infections, hence better methodologies are needed. Here, we report the construction of a promoter probe vector with bioluminescence expression as well as the optimization of a total bacterial RNA extraction protocol to enhance the monitoring of in vivo infections. By

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Sheehan, Gerard, and Kevin Kavanagh. "Proteomic Analysis of the Responses of Candida albicans during Infection of Galleria mellonella Larvae." Journal of Fungi 5, no. 1 (2019): 7. http://dx.doi.org/10.3390/jof5010007.

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This study assessed the development of disseminated candidiasis within Galleria mellonella larvae and characterized the proteomic responses of Candida albicans to larval hemolymph. Infection of larvae with an inoculum of 1 × 106 yeast cells reduced larval viability 24 (53.33 ± 3.33%), 48 (33.33 ± 3.33%) and 72 (6.66 ± 3.33%) h post infection. C. albicans infection quickly disseminated from the site of inoculation and the presence of yeast and hyphal forms were found in nodules extracted from infected larvae at 6 and 24 h. A range of proteins secreted during infection of G. mellonella by C. alb

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Selvaraj, Priya, and Gopal Maheswari. "A Fourier Transform Infrared Spectroscopy Investigation on Faecal Matter of Galleria mellonella Larvae fed with Beeswax and Low-Density Polyethylene." Research Journal of Biotechnology 19, no. 9 (2024): 102–7. http://dx.doi.org/10.25303/1909rjbt1020107.

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The growing production and consumption of plastics, coupled with their poor recyclability and non-degradability, pose significant environmental risks. Insects including the greater wax moth (Galleria mellonella) have been identified as capable of ingesting and degrading plastics through microorganisms in their gut, thereby reducing the environmental impact. The insect that's accountable for degrading down low-density polyethylene has been depicted. The purpose of this study was to analyse the faeces of G. mellonella larvae in order to determine the chemical makeup of their digested products. T

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S., Khater,, El-lakwah, F., Abd-Elmonem, M., Ahmed, A., and Shoukry, F. "Biochemical Virulence of Some Entomopathogenic Nematodes on Galleria mellonella Larvae (Lepidoptera: Galleridae)." Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology 12, no. 2 (2020): 97–109. http://dx.doi.org/10.21608/eajbsc.2020.125099.

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Mannala, Gopala, Beatrice Labat, Guy Ladam, et al. "ANTIBACTERIAL PROPERTIES OF NISIN LAYER-BY-LAYER BASED COATING ON TITANIUM K-WIRES IN GALLERIA MELLONELLA IMPLANT-ASSOCIATED INFECTION MODEL." Orthopaedic Proceedings 106-B, SUPP_19 (2024): 68. http://dx.doi.org/10.1302/1358-992x.2024.19.068.

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AimOrthopedic implants play a tremendous role in fixing bone damages due to aging as well as fractures. However, these implants tend to get colonized by bacteria on the surface, leading to infections and subsequently prevention of healing and osteointegration. Recently, Roupie et al. showed that a nisin layer-by-layer based coating applied on biomaterials has both osteogenic and antibacterial properties. The Galleria mellonella larva is a well-known insect infection model that has been used to test the virulence of bacterial and fungal strains as well as for the high throughput screening of an

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Tao, Ye, Luminita Duma, and Yannick Rossez. "Galleria mellonella as a Good Model to Study Acinetobacter baumannii Pathogenesis." Pathogens 10, no. 11 (2021): 1483. http://dx.doi.org/10.3390/pathogens10111483.

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The invertebrate model, Galleria mellonella, has been widely used to study host–pathogen interactions due to its cheapness, ease of handling, and similar mammalian innate immune system. G. mellonella larvae have been proven to be useful and a reliable model for analyzing pathogenesis mechanisms of multidrug resistant Acinetobacter baumannii, an opportunistic pathogen difficult to kill. This review describes the detailed experimental design of G. mellonella/A. baumannii models, and provides a comprehensive comparison of various virulence factors and therapy strategies using the G. mellonella ho

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Meir, Michal, Tatyana Grosfeld, and Daniel Barkan. "Establishment and Validation of Galleria mellonella as a Novel Model Organism To Study Mycobacterium abscessus Infection, Pathogenesis, and Treatment." Antimicrobial Agents and Chemotherapy 62, no. 4 (2018): e02539-17. http://dx.doi.org/10.1128/aac.02539-17.

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ABSTRACT Treatment of Mycobacterium abscessus infections is extremely challenging due to its intrinsic resistance to most antibiotics, and research of pathogenesis is limited due to a lack of a practical in vivo model of infection. The objective of this study was to establish a simple in vivo model for M. abscessus infection, virulence, and drug testing in Galleria mellonella larvae. We inoculated larvae with M. abscessus bacteria and assessed histopathology, CFU count, and mortality with and without antibiotic treatment. We also constructed a luminescent, recombinant M. abscessus mutant, mDB1

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Eiamthaworn, Kiratiya, David Holthaus, Sureeporn Suriyaprom, Volker Rickerts, and Yingmanee Tragoolpua. "Immunomodulation and Protective Effects of Cordyceps militaris Extract Against Candida albicans Infection in Galleria mellonella Larvae." Insects 15, no. 11 (2024): 882. http://dx.doi.org/10.3390/insects15110882.

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Cordyceps militaris-derived formulations are currently used for multiple purposes because of their medical properties, especially immune system modulation. This study analyzes the inhibitory effects of C. militaris aqueous extract on Candida albicans infections and the immune response in larvae of the greater wax moth Galleria mellonella (Lepidoptera: Pyralidae). Larvae exhibited melanization within 1 h of being infected with C. albicans inoculum at a concentration of 106 cells/larvae, and died within 24 h from a lethal dose. Aqueous extract of C. militaris proved to be nontoxic at concentrati

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Csontos, Attila Sándor, Parwinder S. Grewal, and Michael G. Klein. "Comparative Vertical Migration of Three Different Strains of Heterorhabditis bacteriophora and a Single Strain of Heterorhabditis megidis in Sand at 25°C." Acta Agraria Debreceniensis, no. 11 (September 15, 2003): 47–52. http://dx.doi.org/10.34101/actaagrar/11/3444.

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Heterorhabditis bacteriophora and Heterorhabditis megidis) were tested for downward migration in 9.5 cm vertical sand columns at 25°C with and without a larva of the wax moth, Galleria mellonella, at the bottom. The number of infective juveniles (IJs) in the upper section decreased since the IJs gradually migrated down toward the Galleria larvae into the lower section of the shell vials. Only the isolate OH 25 behaved differently, because the number of IJs decreased this isolate in the lower section. This isolate was the quickest, since after 12 hrs, most IJs could be found in the lower sectio

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Nale, Janet Y., Mahananda Chutia, Jeffrey K. J. Cheng, and Martha R. J. Clokie. "Refining the Galleria mellonella Model by Using Stress Marker Genes to Assess Clostridioides difficile Infection and Recuperation during Phage Therapy." Microorganisms 8, no. 9 (2020): 1306. http://dx.doi.org/10.3390/microorganisms8091306.

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The Galleria mellonella is an effective model for probing Clostridioides difficile interactions with phages. Despite valuable insights from this model, the larvae are not easily amenable to assessing detailed clinical responses to either bacteria or phages. Here, larval survival, colonisation and toxin levels were compared to expression profiles of 17 G. mellonella stress genes to monitor Clostridiodes difficile infection (CDI), and recuperation during phage therapy. The larvae were infected with a ribotype 014/020 isolate and treated with an optimised phage cocktail. Larvae treated prophylact

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Gniazdo, Dawid, Wanesa Sasal, Aleksandra Omelaniuk, Weronika Brudz, and Wiesław Kaca. "Galleria Mellonella Larvae as an In vitro Model for Testing Microbial Pathogenicity." Advancements of Microbiology 62, no. 3-4 (2023): 173–82. http://dx.doi.org/10.2478/am-2023-0015.

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Abstract The larvae of the insect Galleria mellonella have gained wide acceptance as an in vivo model for the study of virulence in Gram-negative and Gram-positive bacteria and fungi. Importantly, G. mellonella offers an alternative, low-cost, and ethically acceptable method of assessing pathogens. These insects have a number of advantages, including ease of breeding, a rapid developmental cycle, low maintenance costs, and ease of experimental manipulation. Most importantly, G. mellonella larvae have a complete immune system and a nonspecific immune response similar to that of humans, making t

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Abbas, T . K. and H. S. Abdulhay. "BIODEGRADATION OF POLYSTYRENE BY PLASTIVORES GREATER WAXWORMS LARVAE (Galleria mellonella)." IRAQI JOURNAL OF AGRICULTURAL SCIENCES 55, no. 1 (2024): 618–25. http://dx.doi.org/10.36103/6p8ryy05.

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This study was aimed to find and test biological methods for reducing the aggregation of plastics such as PS in the environment and study the ability of Greater Wax worms larvae (Galleria mellonella) to eat PS that similar in the its structure to beeswax .Weight loss, morphology changes ,FTIR spectroscopy and GC-mass analysis were performed which showed changes in chemical properties of the PS due to degradation. In this study the percentage of weight loss was 33% in the PS treated with G. mellonella. FTIR of PS frass showed the disappearance of aromatic cycle band that was found in the origin

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Liao, Chunli, Mengdong Zheng, Yushou Chen, Mengjun Wang, and Binging Li. "Immunosuppression mechanism of entomopathogenic bacteria against Galleria mellonella larvae." Process Biochemistry 81 (June 2019): 28–32. http://dx.doi.org/10.1016/j.procbio.2019.03.021.

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Moya-Andérico, Laura, Marija Vukomanovic, Maria del Mar Cendra, et al. "Utility of Galleria mellonella larvae for evaluating nanoparticle toxicology." Chemosphere 266 (March 2021): 129235. http://dx.doi.org/10.1016/j.chemosphere.2020.129235.

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Kloezen, Wendy, Marilyn van Helvert-van Poppel, Ahmed H. Fahal, and Wendy W. J. van de Sande. "A Madurella mycetomatis Grain Model in Galleria mellonella Larvae." PLOS Neglected Tropical Diseases 9, no. 7 (2015): e0003926. http://dx.doi.org/10.1371/journal.pntd.0003926.

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Sehnal, F., A. Fonágy, H. Akai, and H. G. Kallenborn. "Prothoracic glands and ecdysteroid titre in Galleria mellonella larvae." Journal of Insect Physiology 34, no. 7 (1988): 609–14. http://dx.doi.org/10.1016/0022-1910(88)90067-4.

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Cymborowski, B., A. Smietanko, and J. P. Delbecque. "Circadian modulation of ecdysteroid titer in Galleria mellonella larvae." Comparative Biochemistry and Physiology Part A: Physiology 94, no. 3 (1989): 431–38. http://dx.doi.org/10.1016/0300-9629(89)90117-5.

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