Relevant bibliographies by topics / Gap-FRAP

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic 'Gap-FRAP'

Author: Grafiati
Published: 4 June 2021
Last updated: 1 February 2022

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Journal articles on the topic "Gap-FRAP"

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Abbaci, Muriel, Muriel Barberi-Heyob, Jean-René Stines, et al. "Gap junctional intercellular communication capacity by gap-FRAP technique: A comparative study." Biotechnology Journal 2, no. 1 (2007): 50–61. http://dx.doi.org/10.1002/biot.200600092.

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Li, Longkun, Chonghe Jiang, Ping Hao, Weibing Li, Caiping Song, and Bo Song. "Changes of gap junctional cell-cell communication in overactive detrusor in rats." American Journal of Physiology-Cell Physiology 293, no. 5 (2007): C1627—C1635. http://dx.doi.org/10.1152/ajpcell.00122.2007.

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To evaluate the changes in intercellular communication through gap junctions in detrusor overactivity (DO), we studied 23 adult female Wistar rats with DO after partial outflow obstruction (DO group) and 13 sham-operated rats (control group). The two groups were compared by means of urodynamics, light and electron microscopy, expression of Cx40, Cx43, and Cx45 mRNA genes with RT-PCR, Cx43 protein with Western blot analysis, and functional intercellular communication with scrape loading dye transfer (SLDT) and fluorescence recovery after photobleaching (FRAP). The number of gap junctions and the expression of connexin mRNA and Cx43 protein were increased in DO rats, and intercellular communication through gap junctions increased after 6 wk of partial outflow obstruction as assessed with SLDT and FRAP techniques. The findings provide a theoretical rationale for using Cx43 antagonists and gap junction inhibitors in the treatment of patients with overactive detrusor secondary to partial bladder outflow obstruction.
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Kuzma-Kuzniarska, Maria, Clarence Yapp, Thomas W. Pearson-Jones, Andrew K. Jones, and Philippa A. Hulley. "52 IN VITROANDEX VIVOASSESSMENT OF GAP JUNCTION FUNCTION IN TENDON USING FRAP." British Journal of Sports Medicine 48, Suppl 2 (2014): A34.1—A34. http://dx.doi.org/10.1136/bjsports-2014-094114.52.

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Yi, Chenju, Jérémy Teillon, Annette Koulakoff, Hugues Berry, and Christian Giaume. "Monitoring gap junctional communication in astrocytes from acute adult mouse brain slices using the gap-FRAP technique." Journal of Neuroscience Methods 303 (June 2018): 103–13. http://dx.doi.org/10.1016/j.jneumeth.2018.03.005.

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HAN Su-li, 韩素立, 李. 超. LI Chao, 郭. 峰. GUO Feng, and 邵. 晶. SHAO Jing. "Velocity profile measurement of oil films in a confined gap based on FRAP." Optics and Precision Engineering 25, no. 1 (2017): 141–47. http://dx.doi.org/10.3788/ope.20172501.0141.

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Kamioka, Hiroshi, Yoshihito Ishihara, Hans Ris, et al. "Primary Cultures of Chick Osteocytes Retain Functional Gap Junctions between Osteocytes and between Osteocytes and Osteoblasts." Microscopy and Microanalysis 13, no. 2 (2007): 108–17. http://dx.doi.org/10.1017/s143192760707016x.

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The inaccessibility of osteocytes due to their embedment in the calcified bone matrix in vivo has precluded direct demonstration that osteocytes use gap junctions as a means of intercellular communication. In this article, we report successfully isolating primary cultures of osteocytes from chick calvaria, and, using anti-connexin 43 immunocytochemistry, demonstrate gap junction distribution to be comparable to that found in vivo. Next, we demonstrate the functionality of the gap junctions by (1) dye coupling studies that showed the spread of microinjected Lucifer Yellow from osteoblast to osteocyte and between adjacent osteocytes and (2) analysis of fluorescence replacement after photobleaching (FRAP), in which photobleaching of cells loaded with a membrane-permeable dye resulted in rapid recovery of fluorescence into the photobleached osteocyte, within 5 min postbleaching. This FRAP effect did not occur when cells were treated with a gap junction blocker (18α-glycyrrhetinic acid), but replacement of fluorescence into the photobleached cell resumed when it was removed. These studies demonstrate that gap junctions are responsible for intercellular communication between adjacent osteocytes and between osteoblasts and osteocytes. This role is consistent with the ability of osteocytes to respond to and transmit signals over long distances while embedded in a calcified matrix.
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Hu, G. L., Y. D. Fu, Q. L. Zeng, Z. P. Xu, and H. Chiang. "STUDY ON GAP JUNCTIONAL INTERCELLULAR COMMUNICATION INHIBITION BY ELF MAGNETIC FIELDS USING FRAP METHOD." Electromagnetic Biology and Medicine 21, no. 2 (2002): 155–60. http://dx.doi.org/10.1081/jbc-120006787.

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Lemcke, Heiko, Janine Peukert, Natalia Voronina, Anna Skorska, Gustav Steinhoff, and Robert David. "Applying 3D-FRAP microscopy to analyse gap junction-dependent shuttling of small antisense RNAs between cardiomyocytes." Journal of Molecular and Cellular Cardiology 98 (September 2016): 117–27. http://dx.doi.org/10.1016/j.yjmcc.2016.07.008.

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Anders, Juanita J., Suzanne Niedermair, Elaine Ellis, and Maureen Salopek. "Response of rat cerebral cortical astrocytes to freeze- or cobalt-induced injury: An immunocytochemical and gap-FRAP study." Glia 3, no. 6 (1990): 476–86. http://dx.doi.org/10.1002/glia.440030606.

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Alam, Mohammad Khairul, Rumana Tuli, Mohammad Sharif Khan, et al. "Chromatographic Assessment of Polyphenolic Profile and Total Phenolic Content and Antioxidant Activity of Common Leafy Vegetables in Bangladesh." Current Chromatography 7, no. 1 (2020): 40–50. http://dx.doi.org/10.2174/2213240607999200421144940.

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Background: Polyphenolic compounds are known to provide health benefits and protect against degenerative chronic diseases. Utilization and identification of foods with a high content of these compounds are gaining greater attention nowadays. Objective: The present study reports the total phenolic content (TPC), polyphenolic composition and antioxidant activity (DPPH, FRAP and TEAC) of 10 commonly consumed leafy vegetables growing in Bangladesh. Materials and Methods: The samples were collected from different locations of Bangladesh and mixed together to ensure sample representativeness. Folin-Ciocalteu method was used for the analysis of TPC, and quantification of polyphenolic components was done by high-performance liquid chromatography (HPLC- DAD). Additionally, antioxidant activities of the selected vegetables were also analysed by utilizing DPPH, FRAP & TEAC. Results and Discussion: TPC ranged from 23.64 ± 1.20 to 45.59 ± 3.04 mg gallic acid equivalent (GAE)/g freeze-dried sample (fds). The polyphenolic spectrum ranged from 0.30 ± 0.02 to 647.42 ± 147.12 mg/100 g fds; quantity and spectrum of which varied in the vegetables. Among the studied vegetables, Centella asiatica contained the highest amount of TPC (45.59 ± 3.04 mg GAE/g fds) and also exhibited high antioxidant capacities, as documented by DPPH, FRAP and TEAC assays. Moreover, Principal component analysis (PCA) of investigated variables clearly separated Centella asiatica from other samples. Conclusion: Phenolic compounds being strong antioxidants reduce the risk of chronic diseases and the finding of this study would aware the people to take vegetables rich in phenolics. It would also fill up the data gap in the existing food composition table of Bangladesh.
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Dissertations / Theses on the topic "Gap-FRAP"

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Abbaci, Muriel. "Suivi de la fonctionnalité des jonctions communicantes par la technique de gap-FRAP sur des modèles in vitro (2-D, 3-D) et ex vivo : Intérêt pour le diagnostic du cancer." Phd thesis, Université Henri Poincaré - Nancy I, 2008. http://tel.archives-ouvertes.fr/tel-00343150.

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Ce travail de recherche s'inscrit dans un axe de diagnostic du cancer via le développement d'une méthode optique pour la caractérisation fonctionnelle de tissus. La technique de gap- FRAP (Fluorescence Recovery After Photobleaching) permet l'étude quantitative de la fonctionnalité des jonctions gap. La majorité des cellules néoplasiques se caractérisent par une modification du niveau d'expression et/ou de la fonctionnalité des jonctions gap par comparaison à leurs homologues saines. La technique de gap-FRAP permet en conséquence de discriminer les cellules cancéreuses en fonction de la communication intercellulaire gap jonctionnelle (CIGJ). Particulièrement utilisée in vitro, cette technique restait cependant anecdotique ex vivo. Nous avons validé la faisabilité du transfert de cette méthode sur tissus et organes ex vivo. A partir de cellules de statuts différents en expression et en distribution des connexines, nous avons caractérisé la calcéine-AM comme étant une sonde fluorescente adaptée pour des mesures sur tissus. Puis nous avons développé un modèle d'ingénierie systéme pour l'analyse comparative des données de recouvrement de fluorescence sur des modèles bi et tridimensionnels. Nous avons transposé ces conditions préalablement définies sur organe entier ex vivo : la vessie de rat. Un marquage multiple a été optimisé avec une sonde fluorescente pour le tracking des cellules cancéreuses dans la vessie ex vivo, un marqueur pour l'identification histologique de l'urothélium et la calcéine-AM pour mesurer la CIGJ. Le gap-FRAP a été utilisé pour la première fois pour différencier le degré de communication intercellulaire gap jonctionnelle entre le tissu sain et néoplasique sur un organe entier ex vivo, ouvrant des perspectives pour le diagnostic du cancer de la vessie corrélé à la modification de la CIGJ.
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Abbaci, Muriel Guillemin François Barberi-Heyob Muriel. "Suivi de la fonctionnalité des jonctions communicantes par la technique de gap-FRAP sur des modèles in vitro (2-D, 3-D) et ex vivo intérêt pour le diagnostic du cancer /." S. l. : Nancy 1, 2008. http://www.scd.uhp-nancy.fr/docnum/SCD_T_2008_0058_ABBACI.pdf.

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Book chapters on the topic "Gap-FRAP"

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Stout, Randy F., and David C. Spray. "FRAP for the Study of Gap Junction Nexus Macromolecular Organization." In Gap Junction Channels and Hemichannels. CRC Press, 2016. http://dx.doi.org/10.1201/9781315369396-3.

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Conference papers on the topic "Gap-FRAP"

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Abbaci, Muriel, Jean-Rene Stines, Muriel Barberi-Heyob, et al. "In vitro Characterization of Gap Junctional Intercellular Communication by Gap-FRAP Technique." In European Conference on Biomedical Optics. OSA, 2005. http://dx.doi.org/10.1364/ecbo.2005.suc5.

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Abbaci, M., J. R. Stines, M. Barberi-Heyob, et al. "In vitro characterization of gap junctional intercellular communication by gap-FRAP technique." In European Conference on Biomedical Optics 2005, edited by Kai Licha and Rinaldo Cubeddu. SPIE, 2005. http://dx.doi.org/10.1117/12.632829.

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Stines, Jean-Rene, Dominique Dumas, Walter Blondel, Jacques Didelon, and Francois Guillemin. "Discrimination between normal and cancerous cells by gap-FRAP: feasibility in endoscopy." In Photonics Europe, edited by Brian Culshaw, Anna G. Mignani, and Rainer Riesenberg. SPIE, 2004. http://dx.doi.org/10.1117/12.545495.

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Pfau, A., J. Schlienger, D. Rusch, A. I. Kalfas, and R. S. Abhari. "Unsteady Flow Interactions Within the Inlet Cavity of a Turbine Rotor Tip Labyrinth Seal." In ASME Turbo Expo 2003, collocated with the 2003 International Joint Power Generation Conference. ASMEDC, 2003. http://dx.doi.org/10.1115/gt2003-38271.

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This paper focuses on the flow within the inlet cavity of a turbine rotor tip labyrinth seal of a 2 stage axial research turbine. Highly resolved, steady and unsteady 3-dimensional flow data are presented. The probes used here are a miniature 5 hole probe of 0.9mm head diameter and the novel virtual four sensor fast response aerodynamic probe (FRAP) with a head diameter of 0.84mm. The cavity flow itself is not only a loss producing area due to mixing and vortex stretching, it also adversely affects the following rotor passage through the fluid that is spilled into the main flow. The associated fluctuating mass flow has a relatively low total pressure and results in a negative incidence to the rotor tip blade profile section. The dominating kinematic flow feature in the region between cavity and main flow is a toroidal vortex, which is swirling at high circumferential velocity. It is fed by strong shear and end wall fluid from the pressure side of the stator passage. The static pressure field interaction between the moving rotor leading edges and the stator trailing edges is one driving force of the cavity flow. It forces the toroidal vortex to be stretched in space and time. A comprehensive flow model including the drivers of this toroidal vortex is proposed. This labyrinth seal configuration results in about 1.6% turbine efficiency reduction. This is the first in a series of papers focussing on turbine loss mechanisms in shrouded axial turbines. Additional measurements have been made with variations in seal clearance gap. Initial indications show that variation in the gap has a major effect on flow structures and turbine loss.
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Journal articles
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