Journal articles on the topic 'Gas chromatography with electroantennographic detection (GC-EAD-FID)'

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1

Li, Chongyang, Jianmin Cao, Xiufang Wang, Pengjun Xu, Xinwei Wang, and Guangwei Ren. "Efficacy of an improved method to screen semiochemicals of insect." PeerJ 9 (May 20, 2021): e11510. http://dx.doi.org/10.7717/peerj.11510.

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Background A combination of gas chromatography-electroantennographic detection (GC-EAD) and gas chromatography-mass spectrometry (GC-MS) is typically used to screen active compounds that play a role in the regulation of insect behavior. This method uses two kinds of gas chromatography (GC) equipment and needs to compare compounds between the two chromatograms, and it is tedious and costly. To improve detection efficiency, as well as reduce costs and the rate of missed detection, we designed a system connecting gas chromatography (GC), electroantennography (EAG), and mass spectrometry (MS), wit
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2

Tòth, M., C. Löfstedt, and B. S. Hansson. "Epoxyheptadecadienes Identified as Sex Pheromone Components of Tephrina arenacearia Hbn. (Lepidoptera: Geometridae)." Zeitschrift für Naturforschung C 46, no. 3-4 (1991): 257–63. http://dx.doi.org/10.1515/znc-1991-3-416.

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(Z ,Z )-3,9-cis-6,7-Epoxyheptadecadiene, and (Z,Z)-6,9-cis -3,4-epoxyheptadecadiene were identified by gas chromatography/flame ionization detection-electroantennographic detection (GC/FID-EAD) and coupled gas chromatography-mass spectrometry (GC-MS) from abdominal tip extracts of Tephrina arenacearia (Lepidoptera: Geometridae), an alfalfa pest. In gas chromatography/flame ionization-single sensillum detection (GC/FID-SC) analyses, specific olfactory receptor cells were found for (Z,Z)-6,9-cis-3,4-epoxyheptadecadiene, and tricosane. Synthetic samples of racemic (Z,Z )-6,9-cis-3,4-epoxyheptadec
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3

Shuttleworth, Adam, and Steven D. Johnson. "Using two confluent capillary columns for improved gas chromatography‐electroantennographic detection (GC‐EAD)." Entomologia Experimentalis et Applicata 168, no. 2 (2020): 191–97. http://dx.doi.org/10.1111/eea.12873.

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4

Svatoš, Aleš, Blanka Kalinová, Jiří Kindl, et al. "Chemical Characterization and Synthesis of the Major Component of the Sex Pheromone of the Sugarcane Borer Diatraea saccharalis." Collection of Czechoslovak Chemical Communications 66, no. 11 (2001): 1682–90. http://dx.doi.org/10.1135/cccc20011682.

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(9Z,11E)-Hexadeca-9,11-dienal (1) was identified as a major component of the sex pheromone of the sugarcane borer Diatraea saccharalis. The identification is based on mass spectral examination of the extracts prepared from calling females, derivatization experiments with 4-methyl-3,5-dihydro-1,2,4-triazole-3,5-dione (2), stereospecific synthesis of dienal 1, and gas chromatography (GC) with electroantennographic detection (GC-EAD). In GC analysis, the antennaly active component and synthetic dienal 1 show identical retention times on several GC phases. However, the behavioral activity of synth
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Zhang, Mengmeng, Zhihao Cui, Nuo Zhang, Guanglin Xie, Wenkai Wang, and Li Chen. "Electrophysiological and Behavioral Responses of Holotrichia parallela to Volatiles from Peanut." Insects 12, no. 2 (2021): 158. http://dx.doi.org/10.3390/insects12020158.

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Holotrichia parallela (Coleoptera: Scarabaeidae: Melolonthinae) is a notorious pest of many crops, especially peanuts. In this study, volatiles from peanut plants were analyzed using both gas chromatographic-electroantennographic detection (GC-EAD) and gas chromatography/mass spectrometry (GC/MS) techniques, and tested for adult attraction with field trapping bioassays in Hebei Province, China. GC-EAD analyses indicated that H. parallela antennae strongly responded to twelve GC peaks, including eight identified compounds, (Z)-β-ocimene, hexanal, 6-methyl-5-hepten-2-one, nonanal, dihydromyrceno
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Biasazin, Tibebe, Haimanot Chernet, Sebastian Herrera, et al. "Detection of Volatile Constituents from Food Lures by Tephritid Fruit Flies." Insects 9, no. 3 (2018): 119. http://dx.doi.org/10.3390/insects9030119.

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Tephritid fruit flies require protein for sexual and gonotrophic development. Food-based lures are therefore widely used in strategies to detect and control fruit flies in the Tephritidae family. However, these baits are attractive to a broad range of insect species. We therefore sought to identify volatiles detected by the fly antennae, with the goal to compose lures that more specifically target tephritids. Using gas chromatography-coupled electroantennographic detection (GC-EAD) we screened for antennal responses of four important tephritid species to volatile compounds from five commercial
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7

Liu, Yan, Zhihao Cui, Mi Shi, et al. "Antennal and Behavioral Responses of Drosophila suzukii to Volatiles from a Non-Crop Host, Osyris wightiana." Insects 12, no. 2 (2021): 166. http://dx.doi.org/10.3390/insects12020166.

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Drosophila suzukii (Diptera: Drosophilidae) infests a variety of commercial fruits, including cherries and other soft-skinned fruits. After the cropping season of most cultivated crop hosts, it heavily infests the fruit of a wild host-plant, Osyris wightiana in southwest China. Here, we employ gas chromatography-electroantennographic detection (GC-EAD) together with behavioral bioassays and a trapping experiment to identify volatile semiochemicals emitted by O. wightiana that are involved in D. suzukii attraction. GC-EAD recordings of D. suzukii antenna showed responses to 13 compounds, includ
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8

Magsi, Fida Hussain, Zongxiu Luo, Yingjie Zhao, et al. "Electrophysiological and Behavioral Responses of Dasychira baibarana (Lepidoptera: Lymantriidae) to Tea Plant Volatiles." Environmental Entomology 50, no. 3 (2021): 589–98. http://dx.doi.org/10.1093/ee/nvab016.

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Abstract Tea black tussock moth, Dasychira baibarana (Matsumura) (Lepidoptera: Lymantriidae), is a devastating pest species of the tea plant in China. Here, we evaluated the responses of D. baibarana to tea plant volatiles using gas chromatography coupled electroantennographic detection (GC–EAD), eleclectroantennography (EAG), and a Y-tube olfactometer. In total, 11 of 18 analyzed compounds elicited GC–EAD responses from test insects. GC–EAD bio-active compounds were further investigated using EAG and behavioral responses. In the EAG analysis, male moths had significantly greater responses to
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9

Bhanu, K. R. M., D. R. Hall, R. V. Awalekar, et al. "Identification and field evaluation of female sex pheromone of leaf-eating caterpillar, Opisina arenosella (Lepidoptera: Oecophoridae)." International Journal of Tropical Insect Science 38, no. 04 (2018): 274–82. http://dx.doi.org/10.1017/s1742758418000243.

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AbstractThe leaf-eating caterpillar,Opisina arenosellaWalker, is the most destructive pest of coconut palm in India and Southeast Asia. The management practices employed againstO. arenosellaso far have been unsuccessful in many instances in India, due to the pest behaviour and coconut palm phenology. The life cycle, incidence and behaviour ofO. arenosellaare rather interesting and useful for the intervention of pheromone trapping technique for its management. We conducted the present study with the intention of identifying the female sex pheromone ofO. arenosellaand testing its efficacy under
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10

Schiestl, Florian P., Erika A. Wallin, John J. Beck, Magne Friberg, and John N. Thompson. "Generalized olfactory detection of floral volatiles in the highly specialized Greya-Lithophragma nursery pollination system." Arthropod-Plant Interactions 15, no. 2 (2021): 209–21. http://dx.doi.org/10.1007/s11829-021-09809-5.

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AbstractVolatiles are of key importance for host-plant recognition in insects. In the pollination system of Lithophragma flowers and Greya moths, moths are highly specialized on Lithophragma, in which they oviposit and thereby pollinate the flowers. Floral volatiles in Lithophragma are highly variable between species and populations, and moths prefer to oviposit into Lithophragma flowers from populations of the local host species. Here we used gas chromatography coupled with electroantennographic detection (GC-EAD) to test whether Greya moths detect specific key volatiles or respond broadly to
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11

Zhu, Xiaofeng, Bingqiang Xu, Abudukyoum Kader, et al. "Behavioral Responses of Scolytus schevyrewi (Coleoptera: Curculionidae: Scolytinae) to Volatiles From Apricot Tree (Rosales: Rosaceae)." Environmental Entomology 49, no. 3 (2020): 586–92. http://dx.doi.org/10.1093/ee/nvaa027.

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Abstract Scolytus schevyrewi Semenov (Coleoptera: Curculionidae: Scolytinae) is native to China and Central Asia. Damage by the adults and larvae weakens local apricot trees (Armeniaca spp.), often causing death to many host trees. In previous studies, freshly cut apricot logs were found to be highly attractive to S. schevyrewi adults. To explore the possibility of trapping and monitoring this bark beetle, we evaluated the effect of the apricot tree volatiles on S. schevyrewi behavior. Volatiles from the apricot logs were collected by headspace sampling and subjected to coupled gas chromatogra
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12

Liu, Fu, Chengxu Wu, Sufang Zhang, Xiangbo Kong, Zhen Zhang, and Pingyan Wang. "Initial Location Preference Together with Aggregation Pheromones Regulate the Attack Pattern of Tomicus brevipilosus (Coleoptera: Curculionidae) on Pinus kesiya." Forests 10, no. 2 (2019): 156. http://dx.doi.org/10.3390/f10020156.

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Research Highlights: We found that the initial attack location together with the aggregation pheromones played an important role in mediating the aggressive behavior of T. brevipilosus on P. kesiya. Background and Objectives: T. brevipilosus was identified as an aggressive species, which possesses the ability to kill live, healthy P. kesiya. In this scenario, we study the top-down attack pattern of T. brevipilosus on P. kesiya during the entirety of the reproductive period. Materials and Methods: We investigated the phenology of trunk attack on P. kesiya over a period of three years in Pu’er C
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13

Lukas, Kathrin, Tim Harig, Stefan Schulz, Johannes Hadersdorfer, and Stefan Dötterl. "Flowers of European pear release common and uncommon volatiles that can be detected by honey bee pollinators." Chemoecology 29, no. 5-6 (2019): 211–23. http://dx.doi.org/10.1007/s00049-019-00289-x.

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Abstract Floral scents are important pollinator attractants, but there is limited knowledge about the importance of single components in plant–pollinator interactions. This especially is true in crop pollination systems. The aim of this study is to identify floral volatiles of several European pear cultivars (Pyrus communis L.), and to determine their potential in eliciting physiological responses in antennae of honey bees (Apis mellifera L.), the most important pollinators of pear. Volatiles were collected by dynamic headspace and analysed by (high resolution) gas chromatography coupled to ma
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14

Gray, T. G., R. F. Shepherd, G. Gries, and R. Gries. "SEX PHEROMONE COMPONENT OF THE WESTERN BLACKHEADED BUDWORM, ACLERIS GLOVERANA WALSINGHAM (LEPIDOPTERA: TORTRICIDAE)." Canadian Entomologist 128, no. 6 (1996): 1135–42. http://dx.doi.org/10.4039/ent1281135-6.

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Abstract(E)-11,13-Tetradecadienal (E11,13-14:Ald) is the major component of the sex pheromone of the western blackheaded budworm (WBB), Acleris gloverana Walsingham. The compound was identified in extracts of the female’s pheromone gland by coupled gas chromatographic - electroantennographic detection (GC-EAD) and coupled GC - mass spectrometry in selected ion monitoring mode. In field experiments, E11,13-14:Ald by itself was attractive, but addition of (Z)-11,13-tetradecadienal doubled trap catches of male WBB. (E)-11,13-Tetradecadienol or (E)-11,13-tetradecadienyl acetate in binary or ternar
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15

Byers, John A. "Equations for nickel–chromium wire heaters of column transfer lines in gas chromatographic–electroantennographic detection (GC–EAD)." Journal of Neuroscience Methods 135, no. 1-2 (2004): 89–93. http://dx.doi.org/10.1016/j.jneumeth.2003.12.004.

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16

Gries, G., R. Gries, A. L. Perez, A. C. Oehlschlager, L. M. Gonzales, and H. D. Pierce. "Aggregation Pheromone of the African Rhinoceros Beetle, Oryctes monoceros (Olivier) (Coleoptera: Scarabaeidae)." Zeitschrift für Naturforschung C 49, no. 5-6 (1994): 363–66. http://dx.doi.org/10.1515/znc-1994-5-614.

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Abstract Coleoptera, Oryctes monoceros, African Rhinoceros Beetle, Aggregation Pheromone, Ethyl 4-M ethyloctanoate Ethyl 4-m ethyloctanoate is a male-produced aggregation pheromone of the African rhinoc­eros beetle, Oryctes monoceros (Olivier). It was identified by coupled gas chromatographic-electroantennographic detection (GC -EAD) and coupled GC-mass spectrometry (MS) of Porapak Q-trapped male-produced volatiles. Natural and synthetic ethyl 4-m ethyloctanoate elicited comparable antennal responses. In a field experiment (La Me Research Station, Côte d’Ivoire), synthetic ethyl 4-methyloctano
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17

McNair, Crawford, Gerhard Gries, and Regine Gries. "SEX PHEROMONE COMPONENTS OF ENARMONIA FORMOSANA (LEPIDOPTERA: TORTRICIDAE)." Canadian Entomologist 131, no. 1 (1999): 85–92. http://dx.doi.org/10.4039/ent13185-1.

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Abstract(E)-9-Dodecenyl acetate (E9-12:OAc) and (Z)-9-dodecenyl acetate (Z9-12:OAc) are major components of the sex pheromone of the cherry bark tortrix (CBT), Enarmonia formosana (Scopoli), in British Columbia. The compounds were identified in extracts of female pheromone glands by coupled gas chromatographic – electroantennographic detection (GC–EAD) and coupled GC – mass spectrometry. In field experiments, traps baited with E9-12:OAc or Z9-12:OAc singly were unattractive to male CBT, but in combination at ratios of 50:50 or 40:60 captured numerous males. Increasing quantities of this two-co
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18

Judd, Gary J. R., Regine Gries, Carolyn Teasdale, and Gerhard Gries. "Identification of a sex pheromone component for Pennisetia marginata (Lepidoptera: Sesiidae)." Canadian Entomologist 144, no. 6 (2012): 769–78. http://dx.doi.org/10.4039/tce.2012.75.

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AbstractRaspberry crown borer, Pennisetia marginata (Harris) (Lepidoptera: Sesiidae), is a native North American species and pest of many cane fruits. Using coupled gas chromatographic–electroantennographic detection analyses (GC-EAD) we identified candidate sex pheromone components in pheromone gland extracts and effluvia from calling females. Analysis of gland extracts on a Zebron-5 column revealed four components (A, B, C, and D) that elicited strong responses from male antennae. The two most EAD-active components A and B were hypothesised to be (3E,13Z)-octadecadienal [(3E,13Z)-18:Ald] and
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19

Bédard, C., R. Gries, G. Gries, and R. Bennett. "Cydia strobilella (Lepidoptera: Tortricidae): antennal and behavioral responses to host and nonhost volatiles." Canadian Entomologist 134, no. 6 (2002): 793–804. http://dx.doi.org/10.4039/ent134793-6.

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AbstractFemale spruce seed moths, Cydia strobilella (L.) (Lepidoptera: Tortricidae), oviposit on seed cones of most North American spruces (Picea spp.) (Pinaceae) at the time of pollination, and larvae feed on seeds in the maturing cones. We tested the hypothesis that host-seeking moths respond to volatiles from both host and nonhost trees. In coupled gas chromatographic – electroantennographic detection (GC–EAD) analyses of extracts of spruce seed cone volatiles, > 17 compounds elicited antennal responses from male and female C. strobilella. A blend of seven compounds, including (−)-α-pine
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20

Schlyter, Fredrik, Qing-He Zhang, Peter Anderson, et al. "ELECTROPHYSIOLOGICAL AND BEHAVIOURAL RESPONSES OF TOMICUS PINIPERDA AND TOMICUS MINOR (COLEOPTERA: SCOLYTIDAE) TO NON-HOST LEAF AND BARK VOLATILES." Canadian Entomologist 132, no. 6 (2000): 965–81. http://dx.doi.org/10.4039/ent132965-6.

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AbstractLeaf and bark volatiles from non-host birches, Betula pendula Roth. and Betula pubescens Ehrh. (Betulaceae), and aspen, Populus tremula L. (Salicaceae), were tested on spring-dispersing Tomicus piniperda (L.) and Tomicus minor (Hart.) by gas chromatographic – electroantennographic detection (GC–EAD) and by attractant-baited traps in southern Sweden. GC–EAD analysis of the head-space volatiles from fresh bark chips of B. pendula revealed two green leaf alcohols, 1-hexanol and (Z)-3-hexen-1-ol, that consistently elicited antennal responses by T. piniperda and T. minor. Further analyses w
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21

"Semiochemicals of the Scarabaeinae, IV*: Identification of an Attractant for the Dung Beetle Pachylomerus femoralis in the Abdominal Secretion of the Dung Beetle Kheper lamarcki." Zeitschrift für Naturforschung C 50, no. 9-10 (1995): 675–80. http://dx.doi.org/10.1515/znc-1995-9-1013.

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Abstract Both sexes of the dung beetle Pachylomerus femoralis were observed to be attracted to the male dung beetle Kheper lamarcki when it exhibited typical calling behaviour including the release of a white flocculent sex-attracting secretion. Using GC-MS analysis and gas chromatography with electroantennographic (E A D ) and flame ionization detection (FID) in parallel, methyl and ethyl propanoate. methyl and ethyl butanoate, methyl and ethyl pentanoate, and methyl and ethyl 4-pentenoate were identified as the constituents of the secretion that elicited electroantennographic responses in th
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22

Burger, Hannah, Melanie Marquardt, Katharina Babucke, et al. "Neural and behavioural responses of the pollen-specialist bee Andrena vaga to Salix odours." Journal of Experimental Biology 224, no. 13 (2021). http://dx.doi.org/10.1242/jeb.242166.

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ABSTRACT An effective means of finding food is crucial for organisms. Whereas specialized animals select a small number of potentially available food sources, generalists use a broader range. Specialist (oligolectic) bees forage on a small range of flowering plants for pollen and use primarily olfactory and visual cues to locate their host flowers. So far, however, little is known about the specific cues oligoleges use to discriminate between hosts and non-hosts and how floral scent compounds of hosts and non-hosts are processed in the bees' olfactory system. In this study, we recorded physiol
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23

Kühnholz, Susanne, Regine Gries, and John H. Borden. "Semiochemical-based Reproductive Isolation Among Sympatric Species of Trypodendron (Coleoptera: Curculionidae: Scolytinae)." Environmental Entomology, November 13, 2020. http://dx.doi.org/10.1093/ee/nvaa134.

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Abstract Trypodendron retusum (LeConte) (Coleoptera: Curculionidae: Scolytinae) females excised from newly attacked trembling aspen, Populus tremuloides Michaux (Salicaceae), were shown for the first time to produce the aggregation pheromone (+)-lineatin. Coupled gas chromatographic-electroantennographic detection analysis (GC-EAD) disclosed that the antennae of T. retusum, as well as the antennae of three sympatric species, native T. lineatum (Olivier) and T. rufitarsus (Kirby) and exotic T. domesticum (L.), respond to synthetic (+)-lineatin, but not the (−) enantiomer. In contrast, the anten
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