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1

Bestmann, H. J., F. Kern, G. G. Melikyan, et al. "Pheromones, 88 [1]. Sex Pheromone Components of Female Euzophera punicaella M. (Lepidoptera, Pyralidae)." Zeitschrift für Naturforschung C 48, no. 1-2 (1993): 110–12. http://dx.doi.org/10.1515/znc-1993-1-221.

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By means of GC, GC-MS and GC-combined EAG recordings (9Z,12E)-9,12-tetradecadien-1-ol (1) and (9Z,12E)-9,12-tetradecadienyl acetate (2) in a ratio of 4:1 were identified as the pheromone components of the female pyralid moth Euzophera punicaella M. originating from Armenia. Determination of EAG activity with male moth antennae and synthetic test chemicals revealed best responses with a mixture of both compounds.
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2

Ramos, Clécio S., Marisi G. Soares, Adalberto M. da Silva, Luciane G. Batista-Pereira, Arlene G. Corrêa, and Massuo J. Kato. "Electrophysiological Responses of the Naupactus bipes Beetle to Essential Oils from Piperaceae Species." Natural Product Communications 7, no. 8 (2012): 1934578X1200700. http://dx.doi.org/10.1177/1934578x1200700835.

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Electrophysiological responses based on electroantennographic detection (GC-EAD) and electroantennography (EAG) analysis of Naupactus bipes beetles (Germar, 1824) (Coleoptera: Curculionidae: Brachycerinae) were used to test volatile oils of Piper gaudichaudianum, P. regnellii and P. hispidum. In the EAG experiments, female and male beetles showed significant EAG response to the three volatile oils of Piper species, with the females’ responses slightly higher than the males’. The experiments with GC-EAD revealed that some terpenoids (namely, α-pinene, β-pinene, myrcene, α-copaene and germacrene
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3

Li, Chongyang, Jianmin Cao, Xiufang Wang, Pengjun Xu, Xinwei Wang, and Guangwei Ren. "Efficacy of an improved method to screen semiochemicals of insect." PeerJ 9 (May 20, 2021): e11510. http://dx.doi.org/10.7717/peerj.11510.

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Background A combination of gas chromatography-electroantennographic detection (GC-EAD) and gas chromatography-mass spectrometry (GC-MS) is typically used to screen active compounds that play a role in the regulation of insect behavior. This method uses two kinds of gas chromatography (GC) equipment and needs to compare compounds between the two chromatograms, and it is tedious and costly. To improve detection efficiency, as well as reduce costs and the rate of missed detection, we designed a system connecting gas chromatography (GC), electroantennography (EAG), and mass spectrometry (MS), wit
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4

Magsi, Fida Hussain, Zongxiu Luo, Yingjie Zhao, et al. "Electrophysiological and Behavioral Responses of Dasychira baibarana (Lepidoptera: Lymantriidae) to Tea Plant Volatiles." Environmental Entomology 50, no. 3 (2021): 589–98. http://dx.doi.org/10.1093/ee/nvab016.

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Abstract Tea black tussock moth, Dasychira baibarana (Matsumura) (Lepidoptera: Lymantriidae), is a devastating pest species of the tea plant in China. Here, we evaluated the responses of D. baibarana to tea plant volatiles using gas chromatography coupled electroantennographic detection (GC–EAD), eleclectroantennography (EAG), and a Y-tube olfactometer. In total, 11 of 18 analyzed compounds elicited GC–EAD responses from test insects. GC–EAD bio-active compounds were further investigated using EAG and behavioral responses. In the EAG analysis, male moths had significantly greater responses to
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5

Hayashi, Nanao, Akihiko Nishi, Tadayuki Murakami, Kazuyuki Maeshima, Hisashi Komae, and Takashi Sakao. "The Scent Substances of Pierid Butterflies (Hebomoia glaucippe Linnaeus) and the Volatile Components of Their Food Plants (Crataeva religiosa Forst.)." Zeitschrift für Naturforschung C 40, no. 1-2 (1985): 47–50. http://dx.doi.org/10.1515/znc-1985-1-211.

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The scent secretions of two subspecies of Hebomoia glaucippe, which are pierid butterflies, and the essential oils of their food plant were investigated by means of GC and GC-MS. The scent substances of either subspecies are found to be only E-β-ocimene, and the volatile components of Crataeva religiosa were identified as 2-hexanal, 3-hexen-1-ol, p-cymene, limonene, linalool, α-ionone, and β-ionone. Electroantennogram (EAG) were recorded with the antennae of adult male and female butterflies. It was found that EAG responses of male to E-β-ocimene were greater than those of females.
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6

Bestmann, Hans-Jürgen, Athula Attygalle, Hans Platz, Otto Vostrowsky, and Michael Glas. "(Z)-9-Dodecenyl Acetate, a Component of the Sex Pheromone of Cnephasia longana Haworth (Lepidoptera: Tortricidae)." Zeitschrift für Naturforschung C 40, no. 9-10 (1985): 667–69. http://dx.doi.org/10.1515/znc-1985-9-1014.

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Abstract By means of electrophysiological investigations, GC- and GCMS-analysis of gland extracts and GC-analysis with EAG-detection (Z)-9-dodecenyl acetate was proven to be a component of the sex pheromone of female Cnephasia longana moths. (E)-9-dodecenyl acetate acts as a pheromone inhibitor, as shown by field trials.
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7

Bestmann, Hans-Jürgen, Athula B. Attygalle, Thorolf Brosche, et al. "Identification of Three Sex Pheromone Components of the Female Saturniid Moth Antheraea pernyi (Lepidoptera: Saturniidae)." Zeitschrift für Naturforschung C 42, no. 5 (1987): 631–36. http://dx.doi.org/10.1515/znc-1987-0521.

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Abstract By means of electroantennography and single cell recordings, GC and GCMS analyses and GC analysis with EAG detection (6E,11 Z)-6,11-hexadecadienal, (6E,11 Z)-6,11-hexadecadienyl acetate and (4E,9Z)-4,9-tetradecadienyl acetate were identified as the primary components of the sex pheromone of female Antheraea pernyi (Lepidoptera: Saturniidae).
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8

Jham, Gulab N., Alexsandro A. da Silva, Eraldo R. Lima, and Paulo Viana. "Identification (GC and GC-MS) of unsaturated acetates inElasmopalpus lignosellus and their biological activity (GC-EAD and EAG)." Journal of Separation Science 28, no. 3 (2005): 281–85. http://dx.doi.org/10.1002/jssc.200401814.

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9

Būda, Vincas, Sandra Radžiutė, Violeta Apšegaitė, et al. "Electroantennographic and Behavioural Responses of European Cherry Fruit Fly, Rhagoletis cerasi, to the Volatile Organic Compounds from Sour Cherry, Prunus cerasus, Fruit." Insects 13, no. 2 (2022): 114. http://dx.doi.org/10.3390/insects13020114.

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European cherry fruit fly, Rhagoletis cerasi (Diptera: Tephritidae), is the most important pest of sweet and sour cherry fruit. This fly is difficult to control by insecticide application since most of the conventional insecticides used have been banned in Europe. Traps are used for both the pest’s mass trapping and the detection of the beginning of the flight period. Data on flies’ reactions to host-plant volatile organic compounds (VOCs) can be used to search for new attractants. VOCs were collected from the headspace of sour cherry, P. cerasus, fruit. Gas chromatography–mass spectrometry (G
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10

Lahondère, Chloé. "Mosquito Electroantennogram Recordings." Cold Spring Harbor Protocols 2022, no. 9 (2022): PROT107871. http://dx.doi.org/10.1101/pdb.prot107871.

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Electroantennography (EAG) is a simple, yet powerful technique that can be used to indicate that chemical compounds are detected by mosquito antennae. When coupled with gas chromatography (GC), it allows for the identification of detectable compounds within complex mixtures, including those in plant and animal headspaces that might influence mosquito behavior. EAGs and GC-EAGs reveal compounds that can be tested in behavioral assays with the goal of developing new repellents or attractants for mosquito control.
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11

Yang, Meng, Qin Li, Guoshuai Zhao, Yalin Liu, and Yonggen Lou. "Electrophysiological and Behavioral Responses of Orchestes steppensis (Coleoptera: Curculionidae) to Ulmus Plant Volatiles." Plants 14, no. 1 (2024): 42. https://doi.org/10.3390/plants14010042.

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The flea-weevil Orchestes steppensis Korotyaev (Coleoptera: Curculionidae) is an Eastern Palaearctic Steppe species, and a serious pest of elm trees (Ulmus spp., Ulmaceae) by feeding on the leaves (adults) or mining them heavily (larvae) in Xinjiang, China. In order to search for chemical and ecological pest management practices, the olfactory preferences of O. steppensis for leaves of three elm species were investigated. The results revealed that O. steppensis has different host preferences for the three elm species: U. pumila L. first, followed by Ulmus laevis Pall. and Ulmus densa Litw. las
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12

Ma, Jiawei, Ye Tian, Xuli Liu, et al. "Electrophysiological Mechanism and Identification of Effective Compounds of Ginger (Zingiber officinale Roscoe) Shoot Volatiles Against Aphis gossypii Glover (Hemiptera: Aphididae)." Horticulturae 11, no. 5 (2025): 490. https://doi.org/10.3390/horticulturae11050490.

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Aphis gossypii Glover (Homoptera: Aphidinae), a major pest of Chinese pepper (Zanthoxylum bungeanum Maxim), causes significant agricultural damage. Ginger (Zingiber officinale Roscoe) has shown potential as a source for developing botanical pesticides due to its strong bacteriostatic and insecticidal properties; however, the underlying mechanisms remain poorly understood. This study evaluated the repellent activity of ginger shoot extract (GSE) across four solvent phases—petroleum ether, trichloromethane, ethyl acetate, and methanol—against A. gossypii. The results demonstrated that GSE exhibi
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13

Zhao, Dong Xiang, Yu Jie Wang, Jing Lin Gao, Zheng Qiang Peng, Jiong Yin, and Wen Jing Zhu. "Biological Activity and Component Analysis on Body Surface Extracts of Adult Tessaratoma Papillosa." Applied Mechanics and Materials 108 (October 2011): 331–37. http://dx.doi.org/10.4028/www.scientific.net/amm.108.331.

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Preliminary results of petri dish bioassay showed that n-hexane and ether extracts of body surfaces of female and male Tessaratoma papillosa (Drury) were significantly attractive to the opposite sex and the same sex respectively. EAG results also demonstrated that both two kinds of solvent extracts could induce the EAG responses of adult T. papillosa. Sixteen components were identified and isolated from the extracts of adult T. papillosa body surfaces by gas chromatograph-mass spectrometer (GC-MS), of which tridecane was the common component of females and males and had the highest relative am
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14

Grant, G. G., B. Pendrel, K. N. Slessor, X. Z. Meng, and W. E. Miller. "IDENTIFICATION OF SEX PHEROMONE COMPONENTS FOR TWO LEPIDOPTERAN DEFOLIATORS, THE OAK OLETHREUTID LEAFROLLER, PSEUDEXENTERA SPOLIANA (CLEMENS), AND THE ASPEN LEAFROLLER, PSEUDEXENTERA OREGONANA (WALSINGHAM)." Canadian Entomologist 123, no. 6 (1991): 1209–18. http://dx.doi.org/10.4039/ent1231209-6.

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AbstractMoths of the genus Pseudexentera are early-season fliers that include three species of economic importance: P. spoliana (Clemens) on oak (Quercus), P. oregonana (Walsingham) on aspen (Populus), and P. mali Freeman on apple (Malus). GC and GC/MS analysis, EAG and behavioral bioassays, and field tests confirmed that Z10-16:Ac is an attractive sex pheromone component of female P. spoliana. Capillary GC analysis supported by GC/MS also indicated the presence of 16:Ac, Z10-16:OH, and 16:OH in pheromone extracts and there was tentative GC evidence for Z12-16:Ac. However, addition of these co
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15

Koczor, Sándor, József Vuts, John C. Caulfield, et al. "Sex Pheromone of the Alfalfa Plant Bug, Adelphocoris lineolatus: Pheromone Composition and Antagonistic Effect of 1-Hexanol (Hemiptera: Miridae)." Journal of Chemical Ecology 47, no. 6 (2021): 525–33. http://dx.doi.org/10.1007/s10886-021-01273-y.

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AbstractThe sex pheromone composition of alfalfa plant bugs, Adelphocoris lineolatus (Goeze), from Central Europe was investigated to test the hypothesis that insect species across a wide geographical area can vary in pheromone composition. Potential interactions between the pheromone and a known attractant, (E)-cinnamaldehyde, were also assessed. Coupled gas chromatography-electroantennography (GC-EAG) using male antennae and volatile extracts collected from females, previously shown to attract males in field experiments, revealed the presence of three physiologically active compounds. These
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16

Hoskovec, Michal, David Šaman, and Aleš Svatoš. "Synthesis of (8E,10Z)-Tetradeca-8,10-dienal, Sex Pheromone of Horse Chestnut Leafminer (Cameraria ohridella), and All Its Geometrical Isomers." Collection of Czechoslovak Chemical Communications 65, no. 4 (2000): 511–23. http://dx.doi.org/10.1135/cccc20000511.

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(8E,10Z)-Tetradeca-8,10-dienal (1a), sex pheromone of the horse chestnut leafminer (Cameraria ohridella; Lepidoptera, Gracillariidae), and its geometrical isomers (1b-1d) were efficiently synthesized using tetrakis(triphenylphosphine)palladium catalyzed cross-coupling reactions of alk-1-ynes or alkenyl alanes with corresponding vinyl iodides. The stereoisomeric purity of obtained tetradecadienals 1a-1d was higher than 95% (GC). Relative electroantennographic (EAG) activities of the prepared compounds 1a-1d elicited on male antennae supported the previously published identification of the C. oh
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17

Batista-Pereira, Luciane G., João B. Fernandes, M. Fátima G. F. da Silva, Paulo C. Vieira, Odair C. Bueno, and Arlene G. Corrêa. "Electrophysiological Responses of Atta sexdens rubropilosa Workers to Essential Oils of Eucalyptus and its Chemical Composition." Zeitschrift für Naturforschung C 61, no. 9-10 (2006): 749–55. http://dx.doi.org/10.1515/znc-2006-9-1023.

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AbstractThe leaf-cutting ant Atta sexdens rubropilosa Forel, 1908 is the most harmful of the Eucalyptus pests, causing severe losses in wood production through defoliation. Various strategies have been tried and effort spent on the development of methods to control this pest, however no practical and environmentally acceptable one currently exists. In this work the chemical composition of the essential oil of seven Eucalyptus species was identified and the selectivity and sensitivity of antennal receptors of A. sexdens rubropilosa workers to the volatile compounds were determined using the ele
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18

Zhang, Jiangchao, Jinjia Liu, Fei Gao, et al. "Electrophysiological and Behavioral Responses of Apis mellifera and Bombus terrestris to Melon Flower Volatiles." Insects 13, no. 11 (2022): 973. http://dx.doi.org/10.3390/insects13110973.

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As important pollinators, honeybees and bumblebees present a pollination behavior that is influenced by flower volatiles through the olfactory system. In this study, volatile compounds from melon flowers were isolated and identified by headspace solid-phase microextraction (HS-SPME) and gas chromatography–mass spectrometry (GC-MS), and their effects on Apis mellifera and Bombus terrestris were investigated by electroantennogram (EAG) and behavior tests (Y-tube olfactometer). The results showed that 77 volatile compounds were detected in melon flowers, among which the relative content of aldehy
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19

Xue, Jiaqi, Dongdong Ai, Xiangjun Xu, et al. "Isolation and Identification of Volatile Substances with Attractive Effects on Wohlfahrtia magnifica from Vagina of Bactrian Camel." Veterinary Sciences 9, no. 11 (2022): 637. http://dx.doi.org/10.3390/vetsci9110637.

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Vaginal myiasis is one of the most serious parasitic diseases in Bactrian camels. At present, there are no reports on biological control measures of the disease. In this paper, the metabolomic analysis of vaginal secretions from susceptible and non-susceptible camels was performed by ACQUITY UPLC H-Class Ultra Performance Liquid Chromatograph. The results matched in 140 vaginal compounds. Methylheptenone, 1-octen-3-ol, and propyl butyrate and their mixtures were selected for gas chromatography-electroantennography (GC-EAD), electroantennography (EAG), behavioral experiments and trapping experi
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Batista-Pereira, Luciane G., Ellen M. Santangelo, Kathrin Stein, C. Rikard Unelius, Alvaro E. Eiras, and Arlene G. Corrêa. "Electrophysiological Studies and Identification of Possible Sex Pheromone Components of Brazilian Populations of the Sugarcane Borer, Diatraea saccharalis." Zeitschrift für Naturforschung C 57, no. 7-8 (2002): 753–58. http://dx.doi.org/10.1515/znc-2002-7-833.

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Virgin female gland extracts of sugarcane moth Diatraea saccharalis (Fabricius) (Lepidoptera: Pyralidae), from three locations in Brazil, have been analyzed. By GC-MS analysis and comparison of the chromatographic retention time of the components of the pheromone gland with those retention times of synthetic standards, we observed the presence of (Z)- hexadec-11-enal (1), hexadecanal (2), (9E,11Z)-hexadecadienal (4), (9Z,11Z)-hexadecadienal (5) and (9E,11E)-hexadecadienal (6), as minor components besides the major constituent (9Z,11E)-hexadecadienal (3) already reported. We found no variations
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21

Cheng, Jing, Lihong Yin, Shiping Zhou, Min Tang, Yunxian Li, and Fazhong Yang. "The Inhibitory Effect of Powdery Mildew-Induced Volatiles from Rose on Host Selection Behavior of Beet Armyworm Moths (Lepidoptera: Noctuidae)." Journal of Entomological Science 57, no. 1 (2021): 96–113. http://dx.doi.org/10.18474/jes21-13.

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Abstract Plant-mediated indirect interactions (PMIIs) between phytopathogenic fungi and herbivorous insects on shared host plants occur in nature. Knowledge of PMIIs is critical in plant molecular breeding and integrated pest management. We studied the response and chemical mechanism of beet armyworm, Spodoptera exigua (Hübner), adults to rose plants, Rosa chinensis Jacquin, infected with rose powdery mildew, Podosphaera pannosa (Wallr.: Fr.) de Bary. Using gas chromatography-electroantennographic detection (GC-EAD) coupled with electroantennogram (EAG), we found that beet armyworm antennae re
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22

Badji, Cesar Auguste, Jean Dorland, Lynda Kheloul, et al. "Behavioral and Antennal Responses of Tribolium confusum to Varronia globosa Essential Oil and Its Main Constituents: Perspective for Their Use as Repellent." Molecules 26, no. 15 (2021): 4393. http://dx.doi.org/10.3390/molecules26154393.

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Essential oils of aromatic plants represent an alternative to classical pest control with synthetic chemicals. They are especially promising for the alternative control of stored product pest insects. Here, we tested behavioral and electrophysiological responses of the stored product pest Tribolium confusum, to the essential oil of a Brazilian indigenous plant, Varronia globosa, collected in the Caatinga ecosystem. We analyzed the essential oil by GC-MS, tested the effects of the entire oil and its major components on the behavior of individual beetles in a four-way olfactometer, and investiga
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23

Batista-Pereira, Luciane G., Márcio G. dos Santos, Arlene G. Corrêa, et al. "Cuticular Hydrocarbons of Heterotermes tenuis (Isoptera: Rhinotermitidae): Analyses and Electrophysiological Studies." Zeitschrift für Naturforschung C 59, no. 1-2 (2004): 135–39. http://dx.doi.org/10.1515/znc-2004-1-226.

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Termites have become an important pest of Eucalyptus and Pinus reforestations, sugarcane and other cultures. An alternative for the control of this pest would be the use of attractive traps that take in account the social behavior of these insects. Diverse factors are important for the insects in the localization of the habitat and the choice of the food and specific odors can facilitate this. Studies referring to Heterotermes tenuis (Isoptera: Rhinotermitidae) are scarce. The objective of this work was to analyze the tergal cuticular extract of H. tenuis and determine the selectivity and sens
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Kendra, Paul E., Wayne S. Montgomery, Nurhayat Tabanca, et al. "Piperitone (p-Menth-1-En-3-One): A New Repellent for Tea Shot Hole Borer (Coleoptera: Curculionidae) in Florida Avocado Groves." Biomolecules 13, no. 4 (2023): 656. http://dx.doi.org/10.3390/biom13040656.

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The tea shot hole borer, Euwallacea perbrevis, has been recently established in Florida, USA, where it vectors fungal pathogens that cause Fusarium dieback in avocado. Pest monitoring uses a two-component lure containing quercivorol and α-copaene. Incorporation of a repellent into IPM programs may reduce the incidence of dieback in avocado groves, particularly if combined with lures in a push–pull system. This study evaluated piperitone and α-farnesene as potential repellents for E. perbrevis, comparing their efficacy to that of verbenone. Replicate 12-week field tests were conducted in commer
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Jing, Xiaoyuan, Jintong Zhang, Youqing Luo, Shixiang Zong, Peihua Liu, and Junxian Jia. "Identification and Field Evaluation of the Female Sex Pheromone of the Sand Salix Carpenterworm, Holcocerus arenicola Staudinger (Lepidoptera: Cossidae)." Zeitschrift für Naturforschung C 65, no. 5-6 (2010): 403–11. http://dx.doi.org/10.1515/znc-2010-5-614.

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Extracts of female sex pheromone glands of the sand Salix carpenterworm moth, Holcocerus arenicola, a serious pest of desert thicket, were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Based on comparison of retention times and mass spectra of synthetic standards, four compounds were identifi ed as cis-7-tetradecen-1-ol (Z7 - 14:OH), cis-5-tetradecen-1-yl acetate (Z5 - 14:OAc), cis-7-tetradecen- 1-yl acetate (Z7 - 14:OAc), and cis-9-hexadecen-1-yl acetate (Z9 - 16:OAc) with the ratio of 24:39:100:43. Electroantennographic (EAG) analyses of these standard
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Babu, V. R., S. Satpathy, and B. V. S. Reddy. "Identification and characterisation of female released sex pheromone components of jute semilooper, Anomis sabulifera Guenee (Lepidoptera: Noctuidae)." Journal of Environmental Biology 42, no. 2 (2021): 254–64. http://dx.doi.org/10.22438/jeb/42/2/mrn-1501.

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Aim: Identification and characterization of female released sex pheromone components of jute semilooper, Anomis sabulifera Guenee (Lepidoptera: Noctuidae) from female pheromone gland extracts. Methodology: Electroantennogram (EAG) was carried for studying the antennal response; Gas Chromatography coupled with Electro antenna Detector (GC-EAD) was conducted for studying the antennal response of eluted compounds from female pheromone gland extract; Gas Chromatography and Mas Spectrophotometry (GC-MS) was conducted for characterization or getting complete profile of compounds present in the femal
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27

López-Guillén, Guillermo, Leopoldo Cruz-López, and Andrea Joyce. "Attraction of Rhyssomatus nigerrimus (Fahraeus) (Coleoptera: Curculionidae) to Odors from Alternate Host Plants." UTTAR PRADESH JOURNAL OF ZOOLOGY 45, no. 16 (2024): 50–63. http://dx.doi.org/10.56557/upjoz/2024/v45i164287.

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The soybean weevil Rhyssomatus nigerrimus (Fahraeus), is an economically important pest that attacks soybean (Glycine max (L.) Merrill] crops in northern and southern Mexico. Rhyssomatus nigerrimus can be considered a specialist insect because it selects soybean plants for its reproduction and feeding. However, when the rainy season begins, new emerged adult weevils can feed and take refuge in other alternate host plants, such as Rottboellia cochinchinensis (Lour.) W. Clayton, Hyparrhenia rufa (Nees) Stapf, Sorghum halepense (L.) Persoon and Ipomoea trifida (Kunth) G. Don when soybean is not a
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Jintong, Zhang, Han Yan, and Meng Xianzuo. "Sex Pheromone of the Carpenterworm, Holcocerus insularis (Lepidoptera, Cossidae)." Zeitschrift für Naturforschung C 56, no. 5-6 (2001): 423–29. http://dx.doi.org/10.1515/znc-2001-5-617.

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By means of thin-layer chromatography (TLC), electroantennogram (EAG), gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS) and field tests, (Z)-3-tetradecenyl acetate(Z3-14:Ac), (E)-3-tetradecen-1-ol(E3-14:OH), and (Z)-3-tetradecen-1-ol(Z3- 14:OH) at a ratio of 51:39:10 were identified from the female sex pheromone gland extracts of the carpenterworm, Holcocerus insularis Staudinger (Lepidoptera, Cossidae). The average amounts of Z3-14:Ac, E3-14:OH and Z3-14:OH in a single sex pheromone gland of calling moth were 7.29±2.72 ng, 5.72±2.43 ng and 1.44±0.56 ng, respectively. This
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Būda, Vincas, Violeta Apšegaitė, Laima Blažytė-Čereškienė, et al. "Pheromones in Crane Flies: Behaviorally Active Cuticular Compounds in Tipula autumnalis Loew (Diptera: Tipulidae)." Insects 16, no. 1 (2024): 24. https://doi.org/10.3390/insects16010024.

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The pheromones of crane flies (Tipulidae), one of the largest families within the order Diptera (over 15,000 species), are unknown. The aim of our study was to identify the chemical compounds involved in communication in Tipula autumnalis, a representative species of the family. Female cuticular washes were found to be attractive to males in a bioassay. GC-EAD analysis revealed nine EAG-active compounds, which were identified as cuticular hydrocarbons (CHCs). Both males and females contained these CHCs, though in different ratios. The strongest antennal responses in male T. autumnalis were evo
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Li, Peixuan, Yang Wei, Guoxiang Chen, and Adil Sattar. "Perceptual Effects of Walnut Volatiles on the Codling Moth." Insects 15, no. 6 (2024): 402. http://dx.doi.org/10.3390/insects15060402.

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The volatile organic compounds (VOCs) of plant hosts allow insect localization through olfactory recognition. In this study, the oviposition behavior of the codling moth was investigated and the VOCs from different walnut organs were extracted and analyzed to systematically study their composition and content differences. The electrophysiological and behavioral responses of the codling moth to walnut VOCs were measured using gas chromatography–electroantennographic detection (GC-EAD) and a four-arm olfactometer to screen the key active contents. The field investigation results indicated that 9
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Xiao, Dianzhao, Jiahui Liu, Yulong Liu, Yiwei Wang, Yidi Zhan, and Yong Liu. "Exogenous Application of a Plant Elicitor Induces Volatile Emission in Wheat and Enhances the Attraction of an Aphid Parasitoid Aphidius gifuensis." Plants 11, no. 24 (2022): 3496. http://dx.doi.org/10.3390/plants11243496.

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It is well known that plant elicitors can induce plant defense against pests. The herbivore-induced plant volatile (HIPV) methyl salicylate (MeSA), as a signaling hormone involved in plant pathogen defense, is used to recruit natural enemies to protect wheat and other crops. However, the defense mechanism remains largely unknown. Here, the headspace volatiles of wheat plants were collected and analyzed by gas chromatography-mass spectrometry (GC−MS), gas chromatography with electroantennographic detection (GC−EAD) and principal component analysis (PCA). The results showed that exogenous applic
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Dix, M. E., and E. W. Underhill. "SEX PHEROMONE OF RETINIA METALLICA (BUSCK) (LEPIDOPTERA: TORTRICIDAE): IDENTIFICATION AND FIELD STUDIES." Canadian Entomologist 120, no. 8-9 (1988): 721–26. http://dx.doi.org/10.4039/ent120721-8.

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AbstractThe major sex pheromone component from female moths of Retinia metallica (Busck) was identified as Z7,E9-dodecadienyl acetate (Z7,E9-12:Ac) by a combination of electroantennographic (EAG), gas chromatographic (GC), and mass spectrometric analyses. In field tests, males of R. metallica were strongly attracted to Z7,E9-12:Ac. Addition of Z7,Z9-12:Ac, E7,Z9-12:Ac, Z7,E9-dodecadienol (Z7,E9-12:OH), andZ7-dodecenyl acetate (Z7-12:Ac) to Z7,E9-12:Ac did not significantly alter trap catch. However, the addition of E9-dodecenyl acetate (E9-12:Ac) and Z9-12:Ac reduced trap catch. Trap catch was
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33

Torre Alvarez, Ruben, Diana Nolazco Cama, and Lena Tellez Monzón. "Effect of the conditioning and ultrasound-microwave sequential technique on the yield, chemical and functional properties of the essential oils and hydrolats extracted from the leaves of Tagetes elliptica Smith." Scientia Agropecuaria 13, no. 4 (2022): 315–25. http://dx.doi.org/10.17268/sci.agropecu.2022.028.

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Thermal regulation and ultrasound affect the physical, chemical, and antioxidant properties of essential oils and hydrosols extracted by microwaves from the leaves of Tagetes elliptica S. (chincho). The leaves were conditioned by dehydration or freezing, then pre-treated with ultrasound for 15 or 30 minutes at 40 Hz to finally obtain the essential oil (AECH) and hydrolat (HCH) through microwave extraction (500 W and 30 minutes). Yields, phenolic content, antioxidant capacity, leaf structure by optical microscopy, and chemical composition by gas chromatography and mass spectrometry (GC-MS) were
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Blažytė-Čereškienė, Laima, Vincas Būda, Violeta Apšegaitė, et al. "Sea Buckthorn Hippophae rhamnoides and Fruit Flies Rhagoletis batava: Search for Volatile Semiochemicals Involved in Pest Attraction." Horticulturae 8, no. 2 (2022): 179. http://dx.doi.org/10.3390/horticulturae8020179.

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Rhagoletis batava (Diptera: Tephritidae), is the most important pest of Hippophae rhamnoides fruits. For detection and monitoring of R. batava, traps supplied with nonspecific attractants are used. Thus, new, more specific attractants for environment-friendly pest control are needed. Such attractants could be fruit-related semiochemicals that are involved in the host location by flies. Behavioural Y-olfactometer tests revealed that R. batava males were attracted to ripe fruit odour, while females preferred unripe and semi-ripe fruits. Thermal desorption gas chromatography-mass spectrometry (GC
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35

Zhao, Qinglong, Xinning Wang, Ahsan Mustafa, et al. "Varroa Volatiles Offer Chemical Cues to Honey Bees for Initial Parasitic Recognition." Biomolecules 15, no. 1 (2025): 66. https://doi.org/10.3390/biom15010066.

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Olfaction mediated by the antennae is a vital sensory modality for arthropods and could be applied as a tool in pest control. The ectoparasitic mite Varroa destructor poses a significant threat to the health of the honey bee Apis mellifera worldwide and has garnered global attention. To better understand the chemical ecology of this host–parasite relationship, we collected and characterized the volatile organic compounds (VOCs) from V. destructor and used electroantennography (EAG) to record the responses of honey bee (A. c. cerana and A. m. ligustica) antennae to the different VOCs. Fifteen V
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Liu, Jinjia, Min Chen, Weihua Ma, et al. "Composition of Strawberry Flower Volatiles and Their Effects on Behavior of Strawberry Pollinators, Bombus terrestris and Apis mellifera." Agronomy 13, no. 2 (2023): 339. http://dx.doi.org/10.3390/agronomy13020339.

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Strawberries are popular fruits around the world, and their yield and fruit quality rely on pollination by honey bees and bumblebee colonies. Both bee species have their own advantages in strawberry pollination. This study investigates the characteristic of strawberry (Fragaria × ananassa ‘Red Face’) flower volatiles and their effects on bee pollinators by (1) detecting the volatile compounds of strawberry flowers by polydimethylsiloxane (PDMS) combined gas chromatography-mass spectrometry (GC-MS), (2) determining whether Bombus terrestris or Apis mellifera showed antennae responses to certain
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Mancuello Chaparro, Claudia Raquel, Darío Benítez, Yanine Maubet, et al. "Explorando las propiedades de la funga neotropical: perfil químico, actividades antioxidantes y antimicrobianas de Stiptophyllum erubescens (Berk.) Ryvarden." Steviana 14, no. 2 (2023): 5–16. http://dx.doi.org/10.56152/stevianafacenv14n2a1_2022.

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Striptophyllum erubescens es un basidiomiceto neotropical distribuido ampliamente en Sudamérica. Sin embargo, no se han encontrado registros de sus actividades biológicas, por lo que esta investigación constituye el primer estudio del perfil químico y las propiedades antioxidante y antimicrobiana de S. erubescens. Se obtuvo un extracto crudo etanólico y fracciones con disolventes de distintas polaridades (hexano, éter etílico, acetato de etilo y residuo acuoso) del basidioma salvaje. Se realizó la cuantificación de compuestos fenólicos y el ensayo de DPPH. Se analizó la composición de las frac
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Cai, Lijun, Sarina Macfadyen, Baozhen Hua, Wei Xu, and Yonglin Ren. "The Correlation between Volatile Compounds Emitted from Sitophilus granarius (L.) and Its Electrophysiological and Behavioral Responses." Insects 13, no. 5 (2022): 478. http://dx.doi.org/10.3390/insects13050478.

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The granary weevil Sitophilus granarius (L.) is one of the most serious primary insect pests of stored products. When S. granarius present in grains, various volatile organic compounds are released as chemical signals which can be used to detect the insects. In this study, volatile chemical compounds released from S. granarius were analyzed using the headspace solid phase micro-extraction (HS-SPME) coupled with gas chromatography (GC)–mass spectrometry (MS) techniques. Two key compounds, 3-hydroxy-2-butanone and 1-pentadecene, were identified from mixed gender of S. granarius adults at high de
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Affoué Delphine KOUASSI, Fatou Diane Micheline BAGUIA-BROUNE, Kohué Christelle Chantal N’GAMAN-KOUASSI, Janat Akhanovna MAMYRBEKOVA-BEKRO, and Yves-Alain BEKRO. "Mineral and phenolic compositions, antioxidant activity and GC-MS analysis of the leaves of Anchomanes difformis (Blume) Engl. from Côte d’Ivoire." GSC Advanced Research and Reviews 10, no. 1 (2022): 145–55. http://dx.doi.org/10.30574/gscarr.2022.10.1.0035.

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The main objective of this study is to contribute to the valuation of Anchomanes difformis, a plant whose leaves are used for its oxytocic effect at the end of pregnancy and its use in the treatment of rheumatism in Côte d'Ivoire. The X-ray fluorescence spectrometer assay showed that the leaves of Anchomanes difformis contain (mg / 100g): K / 2061; P / 315.2; Ca / 1268; Na / 206.9; Mg / 653.8; Fe / 116.4; Cu / 0.79; Zn / 10.03 and Mn / 68.59. The quantitative spectrophotometric analysis of the phenolic compounds of the hydromethanolic crude extract of the leaves made it possible to determine t
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Affoué, Delphine KOUASSI, Diane Micheline BAGUIA-BROUNE Fatou, Christelle Chantal N'GAMAN-KOUASSI Kohué, Akhanovna MAMYRBEKOVA-BEKRO Janat, and BEKRO Yves-Alain. "Mineral and phenolic compositions, antioxidant activity and GC-MS analysis of the leaves of Anchomanes difformis (Blume) Engl. from Côte d'Ivoire." GSC Advanced Research and Reviews 10, no. 1 (2022): 145–55. https://doi.org/10.5281/zenodo.5954441.

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The main objective of this study is to contribute to the valuation of&nbsp;<em>Anchomanes difformis</em>, a plant whose leaves are used for its oxytocic effect at the end of pregnancy and its use in the treatment of rheumatism in C&ocirc;te d&#39;Ivoire. The X-ray fluorescence spectrometer assay showed that the leaves of&nbsp;<em>Anchomanes difformis</em>&nbsp;contain (mg / 100g): K / 2061; P / 315.2; Ca / 1268; Na / 206.9; Mg / 653.8; Fe / 116.4; Cu / 0.79; Zn / 10.03 and Mn / 68.59. The quantitative spectrophotometric analysis of the phenolic compounds of the hydromethanolic crude extract of
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41

Mergulhão, Naianny, Laisa Carolina Bulhões, Valdemir Silva, Ilza Fernanda Rodrigues, Irinaldo Basílio Júnior, and João Araújo Júnior. "Development of Syzygium aromaticum (L.) essential oil encapsulates by ionic gelification and evaluation of antioxidant activity." Concilium 24, no. 8 (2024): 339–57. http://dx.doi.org/10.53660/clm-3306-24h24.

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The objective of this work was to develop a product capable of protecting Syzygium aromaticum (clove) essential oil against high volatility. Clove oil (CEO) was encapsulated in alginate by ionic gelation with subsequent freeze-drying. Characterization was carried out by GC/MS, TGA/DSC and antioxidant (DPPH), and total phenol (Folin-Ciocalteau) analysis. Eugenol was the majority (81.5%) and the encapsulation efficiency varied between 14.0%+0.96 (F1: 1.0% alginate) and 9.8%+0.35 (F2: 2.0% alginate) in wet encapsulates, and 23.5%+0.14 (LF1: 1.0% alginate) and 16.9%+0.16 (LF2: 2.0% alginate) in fr
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Carson, C., M. A. Birkett, J. G. Logan, et al. "Novel use of stir bar sorptive extraction (SBSE) as a tool for isolation of oviposition site attractants for gravid Culex quinquefasciatus." Bulletin of Entomological Research 100, no. 1 (2009): 1–7. http://dx.doi.org/10.1017/s0007485309006701.

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AbstractMosquitoes such as Culex quinquefasciatus Say (Diptera: Culicidae) are important vectors of organisms that cause disease in humans. Research into the development of effective standardized odour baits for blood-fed females (oviposition attractants), to enable entomological monitoring of vector populations, is hampered by complex protocols for extraction of physiologically active volatile chemicals from natural breeding site water samples, which have produced inconsistent results. Air entrainment and solvent extraction are technically demanding methods and are impractical for use in reso
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Xia, Shike, Tao Zhang, Livy Williams, Yizhong Yang, and Yanhui Lu. "Buckwheat Flower Volatiles Attract Peristenus spretus and Enhance Its Field-Level Parasitism of Apolygus lucorum." Plants 12, no. 8 (2023): 1658. http://dx.doi.org/10.3390/plants12081658.

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Volatile compounds play indispensable roles in the interactions among host plants, herbivores and natural enemies. Previous studies showed that the addition of buckwheat strips in cotton fields could attract Peristenus spretus, the dominant parasitoid of Apolygus lucorum, and enhance its parasitic activity. Through the combined analysis of Y-tube olfactometer, solid-phase microextraction (SPME), gas chromatography-mass spectrometer (GC-MS) and electroantennography (EAG), we found that male and female P. spretus responded to compounds present in buckwheat flowers. The five major components of b
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44

Bustos-Cortés, Jenifer J., Rosa C. Aldana-De la Torre, Anuar Morales-Rodríguez, Carolina Chegwin-Angarita, and Alicia Romero-Frías. "Sexual behavior and chemical signaling in Opsiphanes cassina (Lepidoptera: Nymphalidae)." Chemoecology 33, no. 6 (2023): 161–75. http://dx.doi.org/10.1007/s00049-023-00393-z.

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AbstractBehavior modification using chemical stimuli is a promising tactic for integrated management of lepidopteran pests. Therefore, this approach was evaluated for the management of the butterfly Opsiphanes cassina Felder 1862, a defoliating pest in oil palm plantations. To achieve this aim, we observed adults’ sexual behavior under laboratory conditions and identified glandular structures in the abdomen and hind wings of males that are associated with the production of sexual pheromones in taxonomically related insects. It was also determined that male calls to females occur at 18:00 h whe
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Almeida, Camila Alexandre Cavalcante de, Fernanda Da Silva Gonçalves, Matheus Barros Rodrigues, et al. "Compostos orgânicos voláteis (COVs) cuticulares em Thyrinteina arnobia (Stoll, 1782) (Lepidoptera: Geometridae)." Ciência Florestal 31, no. 2 (2021): 948–58. http://dx.doi.org/10.5902/1980509844521.

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A lagarta-parda do eucalipto, Thyrinteina arnobia (Stoll, 1782) (Lepidoptera: Geometridae), vem sendo relatada de forma significativa em povoamentos florestais. A importância econômica de Thyrinteina arnobia para o setor florestal na atualidade evidencia a necessidade da consolidação do Manejo Integrado de Pragas Florestais (MIP Floresta), incluindo métodos de monitoramento e controle através de semioquímicos, como o emprego de feromônios. Os compostos químicos voláteis (COVs) em substâncias cuticulares de insetos podem contribuir na regulação da interação entre os indivíduos, sendo objeto de
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Barbosa-Cornelio, Ricardo, Fernando Cantor, Ericsson Coy-Barrera, and Daniel Rodríguez. "Tools in the Investigation of Volatile Semiochemicals on Insects: From Sampling to Statistical Analysis." Insects 10, no. 8 (2019): 241. http://dx.doi.org/10.3390/insects10080241.

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The recognition of volatile organic compounds (VOCs) involved in insect interactions with plants or other organisms is essential for constructing a holistic comprehension of their role in ecology, from which the implementation of new strategies for pest and disease vector control as well as the systematic exploitation of pollinators and natural enemies can be developed. In the present paper, some of the general methods employed in this field are examined, focusing on their available technologies. An important part of the investigations conducted in this context begin with VOC collection direct
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Smid, Hans M., Joop J. A. van Loon, Maarten A. Posthumus, and Louise E. M. Vet. "GC-EAG-analysis of volatiles from Brussels sprouts plants damaged by two species of Pieris caterpillars: olfactory receptive range of a specialist and a generalist parasitoid wasp species." Chemoecology 12, no. 4 (2002): 169–76. http://dx.doi.org/10.1007/pl00012665.

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Wang, Yu, Fengyi Wen, Xiangyi Zhou, et al. "Deterrent Effects of Clary Sage Oil and Two Major Constituents against Drosophila suzukii (Diptera: Drosophilidae)." Insects 15, no. 10 (2024): 733. http://dx.doi.org/10.3390/insects15100733.

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Drosophila suzukii (Diptera: Drosophilidae), spotted-wing drosophila, poses a significant threat to soft-skinned fruit crops in the Americas, Europe, Africa, and Oceania, as well as in Asia. The application of chemical insecticides is the primary control strategy for D. suzukii; however, resistance has developed with the indiscriminate use of chemical insecticides. Essential oils, considered potential alternatives to pesticidal strategies, exhibit potent toxic and sublethal behavioral effects against numerous pests, including D. suzukii. Clary sage oil repels a variety of agricultural and hous
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Fujiwara-Tsujii, N., H. Yasui, S. Wakamura, F. Mochizuki, and N. Arakaki. "Age-dependent changes in the ratio of (R)- and (S)-2-butanol released by virgin females of Dasylepida ishigakiensis (Coleoptera: Scarabaeidae)." Bulletin of Entomological Research 102, no. 6 (2012): 730–36. http://dx.doi.org/10.1017/s0007485312000363.

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AbstractThe females of the white grub beetle, Dasylepida ishigakiensis, release two enantiomers of 2-butanol, (R)-2-butanol and (S)-2-butanol. The ratio describing the relative proportions of these two enantiomers (R/S ratio) has not yet been investigated. (R)-2-Butanol has been shown to attract males in laboratory and field experiments, whereas (S)-2-butanol tends to inhibit them. To determine the R/S ratio of the 2-butanol emitted by virgin females, we collected 2-butanol from young (53 days old), mature (63 days old) and old females (73 days old) using water, extracted with an SPME fibre an
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Bhowmik, Biswanath, Udipta Chakraborti, Alivia Mandal, Bishwajeet Paul, and Kakali Bhadra. "Attraction of Aulacophora foveicollis Lucas (Coleoptera: Chrysomelidae) to Host Plant Cucurbita maxima Duchesne (Cucurbitaceae) Volatiles." Agronomy 12, no. 11 (2022): 2640. http://dx.doi.org/10.3390/agronomy12112640.

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The volatiles extracted by the dynamic headspace collection system from the undamaged and conspecific damaged Cucurbita maxima were analyzed by Gas chromatography–mass spectrometry (GC-MS). The olfactory responses of antennal chemosensilla by male and female A. foveicollis towards the plant volatiles were studied by electroantennography (EAG), while the behavioral responses were analyzed by olfactometer bioassay under laboratory conditions. Scanning electron microscopic study revealed the predominance of antennal olfactory sensilla of seven different types with four types of mechanosensilla. T
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