Relevant bibliographies by topics / Gel electrophoresis

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Gel electrophoresis'

Author: Grafiati
Published: 4 June 2021
Last updated: 27 July 2024

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Journal articles on the topic "Gel electrophoresis"

1

Ohshima, Hiroyuki. "Transient Gel Electrophoresis of a Spherical Colloidal Particle." Gels 9, no. 5 (April 23, 2023): 356. http://dx.doi.org/10.3390/gels9050356.

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The general theory is developed for the time-dependent transient electrophoresis of a weakly charged spherical colloidal particle with an electrical double layer of arbitrary thickness in an uncharged or charged polymer gel medium. The Laplace transform of the transient electrophoretic mobility of the particle with respect to time is derived by considering the long-range hydrodynamic interaction between the particle and the polymer gel medium on the basis of the Brinkman–Debye–Bueche model. According to the obtained Laplace transform of the particle’s transient electrophoretic mobility, the transient gel electrophoretic mobility approaches the steady gel electrophoretic mobility as time approaches infinity. The present theory of the transient gel electrophoresis also covers the transient free-solution electrophoresis as its limiting case. It is shown that the relaxation time for the transient gel electrophoretic mobility to reach its steady value is shorter than that of the transient free-solution electrophoretic mobility and becomes shorter as the Brinkman screening length decreases. Some limiting or approximate expressions are derived for the Laplace transform of the transient gel electrophoretic mobility.
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Tan, Timothy Ter Ming, Zong Ying Tan, Wei Liang Tan, and Peng Foo Peter Lee. "Gel electrophoresis." Biochemistry and Molecular Biology Education 35, no. 5 (2007): 342–49. http://dx.doi.org/10.1002/bmb.83.

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Yamanaka, Masamichi. "Supramolecular gel electrophoresis." Polymer Journal 50, no. 8 (March 15, 2018): 627–35. http://dx.doi.org/10.1038/s41428-018-0033-y.

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ünlü, M. "Difference gel electrophoresis." Biochemical Society Transactions 27, no. 4 (August 1, 1999): 547–49. http://dx.doi.org/10.1042/bst0270547.

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Raymond, Samuel. "ACRYLAMIDE GEL ELECTROPHORESIS." Annals of the New York Academy of Sciences 121, no. 2 (December 16, 2006): 350–65. http://dx.doi.org/10.1111/j.1749-6632.1964.tb14208.x.

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Maddox, John. "Understanding gel electrophoresis." Nature 345, no. 6274 (May 1990): 381. http://dx.doi.org/10.1038/345381a0.

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Studier, F. "Slab-gel electrophoresis." Trends in Biochemical Sciences 25, no. 12 (December 1, 2000): 588–90. http://dx.doi.org/10.1016/s0968-0004(00)01679-0.

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Longbottom, David. "Gel electrophoresis: Proteins." Trends in Genetics 10, no. 3 (March 1994): 107. http://dx.doi.org/10.1016/0168-9525(94)90235-6.

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Merrick, B. Alex. "Gel electrophoresis: Proteins." Trends in Cell Biology 4, no. 2 (February 1994): 67–68. http://dx.doi.org/10.1016/0962-8924(94)90016-7.

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Righetti, Pier Giorgio. "Gel electrophoresis: Proteins." Journal of Chromatography A 662, no. 1 (February 1994): 200–201. http://dx.doi.org/10.1016/0021-9673(94)85312-6.

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Dissertations / Theses on the topic "Gel electrophoresis"

1

Hosseini, Seyed Homayoun. "Temperature gradient gel electrophoresis development and application." Diss., Georgia Institute of Technology, 1994. http://hdl.handle.net/1853/25614.

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杜光旭 and Kwong-yuk To. "Electrophoretic behaviour of polystyrene microspheres in agarose gels." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1993. http://hub.hku.hk/bib/B31233247.

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To, Kwong-yuk. "Electrophoretic behaviour of polystyrene microspheres in agarose gels /." [Hong Kong] : University of Hong Kong, 1993. http://sunzi.lib.hku.hk/hkuto/record.jsp?B13465430.

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Askarian, Nameghi Shahnaz. "Genotyping Escherichia coli isolates by Pulsed-Field Gel Electrophoresis." Thesis, Södertörn University College, School of Life Sciences, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-1411.

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Transmission of bacterial strains between patients is a serious problem in hospitals and with the increasing rate of antibiotic resistance the problem has farther escalated. Enterobacteriaceae produced extended-spectrum beta-lactamses (ESBLs), especially Escherichia coli (E-coli), are increasingly important nosocomial pathogens (7, 8). These bacteria are often multiple resistant and are responsible for many intestinal infections and urinary tract infections (2, 5). With the more frequent use of invasive devices in hospital care, these types of nosocomial infections have increased, particularly in seriously ill patients.

In order to diminish transmission of bacterial strains between patients and to study the epidemiology of these bacteria, it is of great importance to develop rapid and specific methods to be able to subtype on strain-level, i.e. to create a fingerprint of the isolates. The method may be based on phenotypic or genotypic characteristics of the microorganism. Any typing method must have high reproducibility and discrimination power to differentiate unrelated strains and also to demonstrate relationship of organisms deriving from the same source. In the present project, a Pulsed-Field Gel Electrophoresis (PFGE) assay for genotyping clinical E. coli isolates was used. PFGE can be used as a genotyping tool and is widely used to type bacteria and trace nosocomial infection. However, the method is time-consuming and relatively expensive in compare with other methods like PCR. In this study, a total of 93 strains were collected. The study was aimed to investigate the genotypes of the collected isolates and to identify and potential the outbreak strains.

The isolates investigated were genotypically diverse shown by a variety of PFGE banding patterns. However, clusters of closely related isolates involved in outbreaks were also identified.

In conclusion, when analyzing a large number of strains, a combination of a rapid phenotyping or genotyping method and a powerful genotyping method like PFGE would be an appropriate strategy for studying clonal relationship among isolates e.g. for detecting cross-transmission of nosocomial pathogens.

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Chan, Hong-Lin. "A 2D-difference gel electrophoresis strategy for redox proteomics." Thesis, University College London (University of London), 2005. http://discovery.ucl.ac.uk/1444604/.

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Post-genomic biomedical science requires quantitative proteomics. In most cases this involves differential protein expression analysis using matched pairs of simultaneously detectable labelling reagents for specific protein amino acids. In this thesis the development and optimisation of a novel cysteine labelling strategy, that is based on the use of iodoacetyl derivatives of Cy3 and Cy5 (ICy3/5) and 2D-difference gel electrophoresis (2D-DIGE) is described. The differentially labelled samples are separated on a single 2D gel and detected by multi-wavelength fluorescence scanning. The method is used to analyse standard proteins and then cell lysates to define the stoichiometry, sensitivity and specificity of this labelling technique. A comparative study of this new proteomic ICy dye protocol with the current NHS-Cy dye labelling system and methods that employ commonly used protein staining methods is described. The method is then used for cysteine labelling of proteins in non-reduced, denatured biological samples allowing accurate monitoring and sensitive detection of redox-dependent thiol modifications and expression level changes. The method is shown to be compatible with the use of MALDI mass spectrometry to identify proteins by analysis of trypsinised ICy labelled peptide digests. Using parallel sample analysis within single gels, the ICy-dye reagents were used to detect redox-, ErbB-2- and growth factor-dependent changes in a human mammary luminal epithelial cell system which was exposed to hydrogen peroxide or to growth factor stimulation. The conventional lysine labelling 2D-DIGE technique was also used in parallel to assess the new ICy labelling strategy for determination of the effects of oxidative stress on protein isoform levels. This study has revealed the identity of proteins involved in the response to oxidative stress and growth factor stimulation in the context of ErbB-2 growth factor receptor over-expression. In addition, this labelling strategy was also used to detect changes in thiol reactivity that follow the UV irradiation of plasma proteins as part of a study designed to evaluate the effect of UV disinfection on plasma product safety for clinical use.
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Stambaugh, Mark P. "Transverse Isotachophoresis Using Polyacrylamide Gel Electrodes." DigitalCommons@CalPoly, 2011. https://digitalcommons.calpoly.edu/theses/505.

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Separation and isolation of a desired analyte from an impure sample solution containing numerous unwanted interfering agents is the first step of nearly every laboratory test performed in medicine and biology. Nucleic acids are often of particular interest to doctors and researchers, and although methods currently exist for their isolation, these procedures are costly in time, man-power, and real-estate. In addition to easing the execution of presently performed tests, mitigation or elimination of these drawbacks would make a large range of currently unperformed tests both practical and feasible. This thesis presents a microfluidics-based approach to the isolation of nucleic acids using transverse isotachophoresis (ITP). A boro-silicate glass chip is used with Poly(Acrylamide) gel electrodes to establish an electric field perpendicular to the direction of sample flow, causing a controlled migration of charged particles. The design and fabrication of the microfluidic chip are addressed, along with the development of a transverse-ITP model which predicts the necessary conditions for the successful separation/concentration of an arbitrary sample. Several proof-of-concept images are provided which demonstrate the effectiveness of transverse ITP using surrogate sample inputs. This thesis proposes a direction for future work which aims toward confirming the model presented and preparing the transverse ITP chip to receive biological samples.
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Xu, Aoshuang. "Development in electrophoresis instrumentation for two-dimensional gel electrophoresis of protein separation and application of capillary electrophoresis in micro-bioanalysis /." [Ames, Iowa : Iowa State University], 2008.

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Rye, Morten Beck. "Image segmentation and multivariate analysis in two-dimensional gel electrophoresis." Doctoral thesis, Norwegian University of Science and Technology, Department of Chemistry, 2007. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-1744.

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The topic of this thesis is data-analysis on images from two-dimensional electrophoretic gels. Because of the complexity of these images, there are numerous steps and approaches to such an analysis, and no “golden standard” has yet been established on how to produce the desired output. In this thesis focus is put on two essential fields concerning 2D-gel analysis; registration of images by segregation and protein spot identification, and data-analysis on the output of such a registration by multivariate methods. Image segmentation is mainly concerned with the task of identifying individual protein spots in a gel-image. This has generally been the natural starting point of all methods and procedures developed since the introduction of 2D-gels in the mid-seventies, simply because this best reproduces the results created by a human analyst, who manually identify protein-spot entities. The amount of data produced in a 2D-gel experiment can be quite large, especially in multiple gels where the human analyst is dependent on additional statistical data-analytical tools to produce results. Because of the correlated nature of most gel-data, analysis by multivariate methods is natural choice, and are therefore adopted in this thesis. The goal of this thesis is to introduce the above mentioned procedures at different stages in the analysis pipeline where they are not yet fully exploited, rather than to improve already existing algorithms. In this way new insight and ideas on how to handle data from 2D-gel experiments are achieved. The thesis starts with a review of segmentation methodology, and introduces a selected procedure used to identify protein spots throughout. Output from the segmentation is then used to create a multivariate spot-filtering model, which aims to separate protein spots from noise and artefacts often creating problems in 2D-gel analysis. Lately the use of common spot boundaries in multiple gels have been the method of choice when gels are analysed. How such boundaries should be defined is an important subject of discussion, and thus a new method for defining common boundaries based on the individual segmentation of each gel is introduced. Segmentation may be a natural starting point when gels are analysed, but it is not necessarily the most correct. Often the introduction of fixed spot entities introduces restrictions to the data which cause problems at later stages in the analysis. Analysing pixels from multiple gels directly has no such restrictions, and it is shown in this thesis that the output of such an analysis based on multivariate methods can produce very useful results. It can also give insight to the data problematic to achieve with the spot boundary approach. At last in the thesis an improved pixel-based approach is introduced, where a less restricted segmentation is used to reduce and concentrate the amount of data analysed, improving the final output.

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Gauthier, Michel. "Modelling a highly biased random walk: Application to gel electrophoresis." Thesis, University of Ottawa (Canada), 2003. http://hdl.handle.net/10393/26334.

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The drift and diffusion motions of biased particles are commonly studied using random walks on lattices. We present a novel theoretical approach that makes it possible to calculate exact mobilities in the presence of lattice obstacles. Several two-dimensional examples are studied and a particular attention is given to separation techniques and how our model can be used to study such devices. We also broach the problems related to the field-dependence of the diffusion coefficient during random walks, and we present new algorithms that remove these difficulties. We develop new Monte Carlo algorithms that make it possible to study both drift and diffusion processes simultaneously, even in presence of very strong fields. Finally, we present two brief discussions about the addition of curved field lines and viscosity gradients to our lattice models. This work opens the door to a wide range of applications, especially for the study of electrophoretic technologies.
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Höök, Helena. "Campylobacter epidemiology : insights from subtyping by pulsed-field gel electrophoresis /." Uppsala : Dept. of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, 2005. http://epsilon.slu.se/200589.pdf.

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Books on the topic "Gel electrophoresis"

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Dunn, M. J. Gel electrophoresis: Proteins. Oxford [England]: Bios Scientific Publishers in association with the Biochemical Society, 1993.

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Ohlendieck, Kay, ed. Difference Gel Electrophoresis. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7268-5.

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Ohlendieck, Kay, ed. Difference Gel Electrophoresis. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2831-7.

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Dunn, Michael J. Gel electrophoresis: Proteins. Oxford: BIOS Scientific Publishers in association with the Biochemical Society, 1993.

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Dunn, Michael J. Gel electrophoresis: Proteins. Oxford: Bios Scientific in association with the Biochemical Society, 1993.

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Margit, Burmeister, and Ulanovsky Levy, eds. Pulsed-field gel electrophoresis. Totowa, N.J: Humana Press, 1992.

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Burmeister, Margit, and Levy Ulanovsky. Pulsed-Field Gel Electrophoresis. New Jersey: Humana Press, 1992. http://dx.doi.org/10.1385/0896032299.

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Jones, P. Gel electrophoresis: Nucleic acids. Chichester: Wiley, 1995.

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Jordan, Kieran, and Marion Dalmasso, eds. Pulse Field Gel Electrophoresis. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2599-5.

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Cramer, Rainer, and Reiner Westermeier, eds. Difference Gel Electrophoresis (DIGE). Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-573-2.

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Book chapters on the topic "Gel electrophoresis"

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Manji, Husseini K., Jorge Quiroz, R. Andrew Chambers, Anthony Absalom, David Menon, Patrizia Porcu, A. Leslie Morrow, et al. "Gel Electrophoresis." In Encyclopedia of Psychopharmacology, 550. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-68706-1_4272.

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Peck, Stewart B., Carol C. Mapes, Netta Dorchin, John B. Heppner, Eileen A. Buss, Gustavo Moya-Raygoza, Marjorie A. Hoy, et al. "Gel Electrophoresis." In Encyclopedia of Entomology, 1587. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6359-6_1041.

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Gooch, Jan W. "Gel Electrophoresis." In Encyclopedic Dictionary of Polymers, 895. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_13810.

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Alphey, Luke. "Gel Electrophoresis." In DNA Sequencing, 53–61. London: Garland Science, 2023. http://dx.doi.org/10.1201/9781003423737-7.

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Engelhardt, Heinz, Wolfgang Beck, and Thomas Schmitt. "Capillary Gel Electrophoresis (CGE)." In Capillary Electrophoresis, 166–85. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-85854-3_8.

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Stellwagen, Nancy C. "DNA Gel Electrophoresis." In Nucleic Acid Electrophoresis, 1–53. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-58924-9_1.

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Frank, J. Howard, J. Howard Frank, Michael C. Thomas, Allan A. Yousten, F. William Howard, Robin M. Giblin-davis, John B. Heppner, et al. "Polyacridamide Gel Electrophoresis." In Encyclopedia of Entomology, 2986. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6359-6_3034.

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Goubet, Florence, Paul Dupree, and Katja Salomon Johansen. "Carbohydrate Gel Electrophoresis." In Methods in Molecular Biology, 81–92. Totowa, NJ: Humana Press, 2010. http://dx.doi.org/10.1007/978-1-61779-008-9_5.

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Mansour, Victoria J., and Jens R. Coorssen. "Quantitative Gel Electrophoresis." In Proteomics in Domestic Animals: from Farm to Systems Biology, 17–35. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-69682-9_3.

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Goubet, Florence, Paul Dupree, and Katja Salomon Johansen. "Carbohydrate Gel Electrophoresis." In Methods in Molecular Biology, 33–44. New York, NY: Springer New York, 2020. http://dx.doi.org/10.1007/978-1-0716-0621-6_2.

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Conference papers on the topic "Gel electrophoresis"

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Lin, David C., Noshir A. Langrana, and Bernard Yurke. "The Migration of DNA Into a DNA-Crosslinked Gel Using Electrophoresis." In ASME 2003 International Mechanical Engineering Congress and Exposition. ASMEDC, 2003. http://dx.doi.org/10.1115/imece2003-43446.

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DNA-crosslinked polyacrylamide gels are polymeric electrolytes by virtue of the fact that DNA is negatively charged in an aqueous solution. As such, their mechanical properties can be altered by electrophoretic and electro-osmotic effects. Hybridization of single-stranded DNA with single-stranded sections of the crosslinks provides a novel means of altering gel mechanical properties. As a step toward exploring this means of altering gel mechanical properties, we report here on a study of the use of electrophoresis to introduce single stranded DNA into DNA crosslinked gels. Changes in elastic properties of the gel, before and after electrophoresis, were measured.
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Akhter, Nazneen, A. R. Khan, Yusuf Talib, Shazia Shadab, and Ruhina Patel. "Analysis of Gel Electrophoresis Images." In 2008 First International Conference on Emerging Trends in Engineering and Technology. IEEE, 2008. http://dx.doi.org/10.1109/icetet.2008.132.

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Mayer, Pascal, Jean Sturm, and G. Weill. "DNA deformation in gel electrophoresis." In Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, edited by Jouko E. Korppi-Tommola. SPIE, 1993. http://dx.doi.org/10.1117/12.146161.

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Matsumoto, Mitsuhiro, and Masao Doi. "Dynamics of DNA in gel electrophoresis." In Slow dynamics in condensed matter. AIP, 1992. http://dx.doi.org/10.1063/1.42466.

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"MATCHING TWO-DIMENSIONAL GEL ELECTROPHORESIS’ SPOTS." In International Conference on Bioinformatics Models, Methods and Algorithms. SciTePress - Science and and Technology Publications, 2012. http://dx.doi.org/10.5220/0003702401110117.

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David, Regis A., Justin L. Black, Brian D. Jensen, and Sandra H. Burnett. "Modeling and Experimental Validation of DNA Motion During Electrophoresis." In ASME 2010 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2010. http://dx.doi.org/10.1115/detc2010-28541.

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This paper describes the protocol and presents the results for DNA motion experiments using fabricated macroscale gel electrophoresis devices. Gel electrophoresis is a process used to separate/move DNA, RNA or protein molecules using an electric field through a gel matrix (electrolytic solution). In electrolytic solutions, the current conduction is due to a transport of ions (anions and cations). A better understanding of electrophoretic fundamentals allows for modeling the motion of DNA during electrophoresis. The model is validated through comparison with the experimental results. The model and experimental validation will be used to improve the process of cellular nanoinjection of DNA, currently in development in our lab.
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Kaya, Deniz Ece, Tanıl Kocagöz, Sesin Kocagöz, and Cengizhan Öztürk. "Observable Real-Time Pulsed-Field Gel Electrophoresis." In 2017 21st National Biomedical Engineering Meeting (BIYOMUT). IEEE, 2017. http://dx.doi.org/10.1109/biyomut.2017.8479196.

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Mese, Alev Kakac, Aykut Erdamar, and Ozlem Darcansoy Iseri. "Image analysis for single cell gel electrophoresis." In 2017 25th Signal Processing and Communications Applications Conference (SIU). IEEE, 2017. http://dx.doi.org/10.1109/siu.2017.7960416.

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Park, Sang Cheol, In Seop Na, Soo Hyung Kim, Guee Sang Lee, Kang Han Oh, Jeong Hwan Kim, and Tae Ho Han. "Lanes Detection in PCR Gel Electrophoresis Images." In 2011 IEEE 11th International Conference on Computer and Information Technology (CIT). IEEE, 2011. http://dx.doi.org/10.1109/cit.2011.89.

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de Carmejane, Olivia, Jeffrey J. Schwinefus, and Michael D. Morris. "Plasmid topoisomer separation by capillary gel electrophoresis." In BiOS '99 International Biomedical Optics Symposium, edited by Joseph R. Lakowicz, Steven A. Soper, and Richard B. Thompson. SPIE, 1999. http://dx.doi.org/10.1117/12.347533.

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Reports on the topic "Gel electrophoresis"

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Uberbacher, E. C., and G. J. Bunick. Purification of nucleoprotein particles by elution preparative gel electrophoresis. Office of Scientific and Technical Information (OSTI), September 1986. http://dx.doi.org/10.2172/5127796.

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R. JOHNSTON. THERMAL DETECTION OF DNA AND PROTEINS DURING GEL ELECTROPHORESIS. Office of Scientific and Technical Information (OSTI), August 2000. http://dx.doi.org/10.2172/768736.

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Xu, Aoshuang. Development in electrophoresis: instrumentation for two-dimensional gel electrophoresis of protein separation and application of capillary electrophoresis in micro-bioanalysis. Office of Scientific and Technical Information (OSTI), January 2008. http://dx.doi.org/10.2172/1342558.

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Dunn, Bruce E., Martin J. Blaser, and Edward L. Snyder. Two-Dimensional Gel Electrophoresis and Immunoblotting of Campylobacter Outer Membrane Proteins. Fort Belvoir, VA: Defense Technical Information Center, April 1987. http://dx.doi.org/10.21236/ada265461.

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Gaul, Stephen B., Stephanie Wedel, Matthew M. Erdman, D. L. Hank Harris, Isabel Turney Harris, Kathleen E. Ferris, and Lorraine J. Hoffman. Identification of Swine Salmonella serotypes Using Pulsed-field Gel Electrophoresis of Conserved Xba1 Fragments. Ames (Iowa): Iowa State University, January 2007. http://dx.doi.org/10.31274/ans_air-180814-809.

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Yinfa, Ma. Indirect fluorometric detection techniques on thin layer chromatography and effect of ultrasound on gel electrophoresis. Office of Scientific and Technical Information (OSTI), December 1990. http://dx.doi.org/10.2172/6045672.

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Little, Stephen F. Western Blot Analysis of the Exotoxin Components From Bacillus anthracis Separated by Isoelectric Focusing Gel Electrophoresis. Fort Belvoir, VA: Defense Technical Information Center, February 2004. http://dx.doi.org/10.21236/ada435242.

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McGregor, David A. Optimization of separation and detection schemes for DNA with pulsed field slab gel and capillary electrophoresis. Office of Scientific and Technical Information (OSTI), July 1993. http://dx.doi.org/10.2172/10116369.

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Dai, H., S. Sanderson, J. Davey, F. Uribe, and T. A. Jr Zawodzinski. Electrophoretic NMR measurements of lithium transference numbers in polymer gel electrolytes. Office of Scientific and Technical Information (OSTI), May 1997. http://dx.doi.org/10.2172/474865.

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Fisk, D. J., and J. C. Sutherland. A laser scanner for imaging fluorophore labeled molecules in electrophoretic gels. Office of Scientific and Technical Information (OSTI), August 1995. http://dx.doi.org/10.2172/95218.

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