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Relevant bibliographies by topics / Gel filtration chromatography

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Academic literature on the topic 'Gel filtration chromatography'

Author: Grafiati

Published: 4 June 2021

Last updated: 20 February 2023

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Journal articles on the topic "Gel filtration chromatography"

1

Werner, Milton H., G. Marius Clore, Angela M. Gronenborn, Akiko Kondoh, and Robert J. Fisher. "Refolding proteins by gel filtration chromatography." FEBS Letters 345, no. 2-3 (May 30, 1994): 125–30. http://dx.doi.org/10.1016/0014-5793(94)00401-3.

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Barnes, Ana Isabel, Cristina Ortiz, María Gabriela Paraje, Luis Eduardo Balanzino, and Inès Albesa. "Purification and characterization of a cytotoxin fromEnterobacter cloacae." Canadian Journal of Microbiology 43, no. 8 (August 1, 1997): 729–33. http://dx.doi.org/10.1139/m97-105.

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Leukotoxic activity was assayed in clinical isolates of Enterobacter cloacae. Two strains were selected out of 38 by their greater hemolytic activity in blood agar plates. Leukotoxin was purified by salt precipitation, dialysis, chromatography by gel filtration, and high pressure liquid chromatography (HPLC). Human leukocytes, when incubated with purified E. cloacae toxin, showed high percentages of death and lysis, with time and dose dependence. The chromatographic profile of gel filtration presented three protein peaks and toxic activity was detected in the second peak. After HPLC, leukotoxi

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Hunt, Eric A., and Sapna K. Deo. "Board-Game Gel Filtration and Affinity Chromatography." Journal of Chemical Education 86, no. 1 (January 2009): 19. http://dx.doi.org/10.1021/ed086p19.

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Wooten, Arthur L., M. Lynn Prewitt, Terry Sellers, and David C. Teller. "Gel filtration chromatography of resole phenolic resins." Journal of Chromatography A 445 (January 1988): 371–76. http://dx.doi.org/10.1016/s0021-9673(01)84549-0.

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Girona, V., J. Estelrich, M. Pujol, and J. Bolòs. "Ampicillin polymers: identification by gel-filtration chromatography." International Journal of Pharmaceutics 41, no. 3 (February 1988): 241–44. http://dx.doi.org/10.1016/0378-5173(88)90200-1.

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Kumar Shaha, Ranajit, Nitai Roy, and Talukdar G. "Characterization and Sensitivity Test of the Allergenic Pollen Proteins from Litchi Chimensis Plant." Journal of Tropical Resources and Sustainable Science (JTRSS) 1, no. 1 (August 15, 2021): 25–35. http://dx.doi.org/10.47253/jtrss.v1i1.667.

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Pollen of Litchi chinensis (Litchi) is a major aeroallergen of Bangladesh. Pollen of this fruits plant was collected from full bloomed flower growing in different places of Rajshahi in Bangladesh. Pollen protein was extracted and partial purified by means of long-term PBS extraction, salting out, dialysis, gel filtrations and DEAE-Cellulose chromatography and the protein was designated as LFPP (Litchi flowers pollen protein). Gel filtration of the purified pollen protein gives two main peaks. The major peak gives four bands on SDS-PAGE. The enzyme (pectate lyase) proteins after gel filtration

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Russo, Salvatore F., and Angie Radcliffe. "Separations utilizing gel filtration and ion-exchange chromatography." Journal of Chemical Education 68, no. 2 (February 1991): 168. http://dx.doi.org/10.1021/ed068p168.

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González, Ana, and Jaime Gómez-Márquez. "Purification of bacteriophage DNA by gel filtration chromatography." Gene Analysis Techniques 7, no. 1 (February 1990): 2–4. http://dx.doi.org/10.1016/0735-0651(90)90037-g.

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Whisenant, E. C., B. K. A. Rasheed, and Y. M. Bhatnagar. "Plasmid purification using high-performance gel filtration chromatography." Nucleic Acids Research 16, no. 11 (1988): 5202. http://dx.doi.org/10.1093/nar/16.11.5202.

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Nitoda, Teruhiko, Hirokazu Usuki, and Hiroshi Kanzaki. "A Potent Insect Chitinase Inhibitor of Fungal Origin." Zeitschrift für Naturforschung C 58, no. 11-12 (December 1, 2003): 891–94. http://dx.doi.org/10.1515/znc-2003-11-1226.

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Abstract A water-soluble polysaccharide was isolated from the culture filtrate of a fungal strain, Sphaeropsis sp. TNPT116-Cz, as a novel insect chitinase inhibitor. It was purified to chromatographic homogeneity by ethanol precipitation, anion-exchange and gel filtration chromatography. Its molecular weight was estimated to be 16 kDa by gel filtration HPLC. Monosaccharide analysis showed that it contained glucose, galactose, N-acetylglucosamine and a deoxysugar. This polysaccharide showed potent and specific inhibitory activity against Spodoptera litura chitinase with an IC50 value of 28 nᴍ.

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Dissertations / Theses on the topic "Gel filtration chromatography"

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Zhang, Qunying. "Characterization of receptors for Escherichia coli 987P using competitive binding assays, thin-layer chromatography and gel filtration chromatography." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0023/MQ51825.pdf.

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Ring, Ludwig. "Purification of psychoactive biomolecules in plants using size exclusion chromatography." Thesis, Linköping University, Department of Physics, Chemistry and Biology, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-18434.

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<p><em>Size exclusion chromatography</em> (SEC) was applied for purification of psychoactive biomolecules from plants. These molecules are in the same molecular weight range, but do not necessarily share other chemical properties, that makes the SEC technique efficient. By applying SEC as a first purification step much of the co-extractives from the plants can easily be removed. Large amounts of target substance can be obtained with little effort if the system is automated. Combining SEC with a second purification step, consisting of normal phase chromatography, provides high purity of the tar

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Gustafsson, Sofia. "Expression and Purification of Murine Tripeptidyl Peptidase II." Thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-177009.

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Tripeptidyl peptidase II (TPPII) is an exopeptidase which cleaves tripeptides from theN-terminus of peptides. The exact functional role of TPPII is still a matter of investigation. Itis believed that the enzyme is primarily involved in intracellular protein degradation, where itcooperates with the proteasome and other peptidases to degrade proteins into free aminoacids. These amino acids can subsequently be used in the production of new proteins. The aimof this work was to express murine wild type TPPII using E. coli and thereafter purify theenzyme from the bacterial lysate. Methods used for t

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Suttisansanee, Uthaiwan. "Biochemistry in Bacterioferritin." Thesis, University of Waterloo, 2006. http://hdl.handle.net/10012/2983.

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Bacterioferritin, an iron storage protein having a 24-subunit quaternary structure, was used as a model for the study of host-guest interactions and guest encapsulation, making use of its spherical cage-like structure. A hexahistidine-affinity tag fused to the C-terminus of each bacterioferritin subunit was constructed. The C-terminus of each subunit points toward the inside of the cavity, while the N-terminus is exposed on the surface of the protein. The hexaHistag was able to form strong interactions with a nickel-nitrilotriacetic acid linked dye molecule (guest) and this interactio

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Vergnolle, Chantal. "I: purification et caracterisation de proteines de transfert de phospholipides, a partir de feuilles d'epinard (spinacia oleracea l. ). Ii: synthese in vitro des proteines vegetales : methodologie." Paris 6, 1986. http://www.theses.fr/1986PA066254.

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Des proteines, capables de faciliter des mouvements intermembranaires de phospholipides, ont ete isolees a partir de feuilles d'epinard. Ces proteines, appelees proteines de transfert de phospholipides, ont ete purifiees par les techniques classiques de chromatographie (filtration sur gel, echangeurs d'ions) ou par les techniques plus resolutives et plus rapides de chromatographie liquide a haute performance (colonnes echangeuses d'ions ou en phase inverse). Nous avons verifie la purete des fractions par electrophorese en presence de sds

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Trevisoli, Edilaine Della Valentina Gonçalves. "Purificação de eliciadores de defesa vegetal em soja e feijoeiro a partir de nematoides fitopatogênicos." Universidade Estadual do Oeste do Paraná, 2016. http://tede.unioeste.br:8080/tede/handle/tede/1475.

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Made available in DSpace on 2017-07-10T17:40:55Z (GMT). No. of bitstreams: 1 Edilaine_D_V_GoncalvesTrevisoli.pdf: 2707364 bytes, checksum: 7b7a664f9727d5909c470f377ee155e8 (MD5) Previous issue date: 2016-02-25<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>The induction of resistance in plants to pathogens is an alternative method of disease control, wich involves activation of plant resistance mechanisms such as induction of phytoalexins. The elicitors molecules are able to induce and activate those responses, and therefore, techniques have sought to isolate and charact

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Baba, Hamed Mohamed Bey. "Purification et caractérisation de protéases alcalines des larves de Galleria Mellonella." Rouen, 1986. http://www.theses.fr/1986ROUES053.

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Deux protéases alcalines P1 et P2 ont été isolées à partir d'homogénats de larves de Galleria mellonella. Les 2 enzymes ont été séparées par chromatographie sur échangeurs d'ions et purifiées ultérieurement par filtration sur gel. Les 2 protéases se distinguent par leur pH optimum d'action, leur poids moléculaire et leur sensibilité vis-a-vis de différents inhibiteurs de protéases. La répartition anatomique de l'activité protéolytique montre la présence de 2 protéases alcalines similaires à P1 et P2 dans le tube digestif, et d'une protéase similaire à P1 dans le tissu adipeux

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Meinerz, Cristiane Claudia. "Indução de mecanismos bioquímicos de defesa em sorgo (Sorghum bicolor) por frações obtidas do decocto de avenca (Adiantum capillus-veneris)." Universidade Estadual do Oeste do Paraná, 2010. http://tede.unioeste.br:8080/tede/handle/tede/1413.

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Made available in DSpace on 2017-07-10T17:37:44Z (GMT). No. of bitstreams: 1 Cristiane_Claudia_Meinerz.pdf: 977726 bytes, checksum: 47602589d307d4398cc6061bcd0d1eeb (MD5) Previous issue date: 2010-02-24<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>Induction of resistance involves the activation of plant defense mechanisms in response to treatment with biotic or abiotic elicitors. The application of plant extracts in order to induce resistance mechanisms is an interesting alternative to chemical control, however, besides the presence of inducers, can occur the presence

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Kruger, Sarah Jane, and n/a. "Characterisation of Proteins from Grevillea robusta and NMR Studies of the Serine Protease Inhibitor." Griffith University. School of Science, 2004. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20040618.150708.

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Proteins that recognise the sugar surface structures on cells have an enormous potential to be used as tools in the characterisation of these structures. A group of proteins, called lectins, have been identified that can bind to carbohydrate complexes on the receptors of cells. The crude extract from Grevillea robusta seeds was found to contain lectin-like proteins that were different from most other lectins, as they would specifically target the receptors of white blood cells and not those found on red blood cells. Therefore, the lectin isolated from G.robusta could be used as a tool to id

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Kruger, Sarah Jane. "Characterisation of Proteins from Grevillea robusta and NMR Studies of the Serine Protease Inhibitor." Thesis, Griffith University, 2004. http://hdl.handle.net/10072/366534.

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Proteins that recognise the sugar surface structures on cells have an enormous potential to be used as tools in the characterisation of these structures. A group of proteins, called lectins, have been identified that can bind to carbohydrate complexes on the receptors of cells. The crude extract from Grevillea robusta seeds was found to contain lectin-like proteins that were different from most other lectins, as they would specifically target the receptors of white blood cells and not those found on red blood cells. Therefore, the lectin isolated from G.robusta could be used as a tool to id

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Books on the topic "Gel filtration chromatography"

1

Harkin, J. M., Helmut Determann, and E. Gross. Gel Chromatography Gel Filtration · Gel Permeation · Molecular Sieves: A Laboratory Handbook. Springer London, Limited, 2012.

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Determann, Helmut. Gel Chromatography : Gel Filtration · Gel Permeation · Molecular Sieves: A Laboratory Handbook. Springer London, Limited, 2013.

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Determann, Helmut. Gel Chromatography Gel Filtration · Gel Permeation · Molecular Sieves: A Laboratory Handbook. Springer, 2012.

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Determann, Helmut. Gel Chromatography: Gel Filtration · Gel Permeation · Molecular Sieves a Laboratory Handbook. Springer London, Limited, 2012.

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5

Kirkland, Joseph J., Andre Striegel, Wallace W. Yau, and Donald D. Bly. Modern Size-Exclusion Liquid Chromatography: Practice of Gel Permeation and Gel Filtration Chromatography. Wiley & Sons, Incorporated, John, 2009.

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Kirkland, Joseph J., Andre Striegel, Wallace W. Yau, and Donald D. Bly. Modern Size-Exclusion Liquid Chromatography: Practice of Gel Permeation and Gel Filtration Chromatography. Wiley & Sons, Limited, John, 2009.

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Book chapters on the topic "Gel filtration chromatography"

1

Ó’Fágáin, Ciarán, Philip M. Cummins, and Brendan F. O’Connor. "Gel-Filtration Chromatography." In Methods in Molecular Biology, 25–33. Totowa, NJ: Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-913-0_2.

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Ó’Fágáin, Ciarán, Philip M. Cummins, and Brendan F. O’Connor. "Gel-Filtration Chromatography." In Methods in Molecular Biology, 15–25. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6412-3_2.

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Moore, Peter M. "Gel Filtration Chromatography." In Adsorption: Science and Technology, 561–76. Dordrecht: Springer Netherlands, 1989. http://dx.doi.org/10.1007/978-94-009-2263-1_29.

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Kummari, Raghupathi, and Kakoli Bose. "Gel Filtration Chromatography." In Textbook on Cloning, Expression and Purification of Recombinant Proteins, 199–219. Singapore: Springer Singapore, 2022. http://dx.doi.org/10.1007/978-981-16-4987-5_8.

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Hagel, Lars. "Gel Filtration: Size Exclusion Chromatography." In Methods of Biochemical Analysis, 51–91. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2011. http://dx.doi.org/10.1002/9780470939932.ch3.

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Page, Mark, and Robin Thorpe. "Purification of IgG Using Gel-Filtration Chromatography." In Springer Protocols Handbooks, 735–37. Totowa, NJ: Humana Press, 1996. http://dx.doi.org/10.1007/978-1-60327-259-9_131.

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Booy, Evan P., Hui Meng, and Sean A. McKenna. "Native RNA Purification by Gel Filtration Chromatography." In Recombinant and In Vitro RNA Synthesis, 69–81. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-113-4_6.

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Bai, Yan. "Detecting Protein-Protein Interactions by Gel Filtration Chromatography." In Methods in Molecular Biology, 223–32. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2425-7_13.

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Elwell, Lynn P. "A Rapid Method for Purifying Escherichia coli β-galactosidase Using Gel-Filtration Chromatography." In Filtration and Purification in the Biopharmaceutical Industry, 467–80. Third edition. | Boca Raton, Florida : CRC Press, 2019. | Series: Drugs and the pharmaceutical sciences: CRC Press, 2019. http://dx.doi.org/10.1201/9781315164953-18.

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Masoodi, Khalid Z., Sameena Maqbool Lone, and Rovidha Saba Rasool. "Gel-filtration or size-exclusion chromatography." In Advanced Methods in Molecular Biology and Biotechnology, 147–49. Elsevier, 2021. http://dx.doi.org/10.1016/b978-0-12-824449-4.00026-8.

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Conference papers on the topic "Gel filtration chromatography"

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Xiong, Yi-min, Zhen-yi Wang, Ye-lu Xu, and Chenq-wu Chi. "REVERSIBLE BINDING OF THE TISSUE PLASMINOGEN ACTIVATOR WITH FIBRONECTIN." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644404.

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Fresh pig heart tissues were homogenized and deli-pidized with cold acetone. The dry acetone powder was extracted with 0.45 M potassium acetate pH 4.5, the extract was fractionated with ammonium sulfate to 60% saturation, successively followed by four chromatography steps: chromatography on CM-Sepharose CL-6B; gel filtration on Sephadex G-100; Fibrin-Sepharose affinity chromatography and chromatography on DEAE-Sepharose CL-6B. The fibrin plate method was used for the tissue plasminogen activator (t-PA) activity determination. The porcine t-PA purified was proved to be homogeneous either by SDS

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Carlsson, I., J. Chmielewska, and B. Wiman. "ON DIFFERENT MOLECULAR EORMS OE PLASMINOGEN ACTIVATOR INHIBITOR." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644434.

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The production of plasminogen activator inhibitor (PAI) by the human cell-lines Hep G2 and HT 1080 have been studied by immunochemical and functional methods. In conditioned medium collected after 2h, the PAI seemed to be almost fully active, but with increasing incubation time the activity was gradually lost, in spite of that the PAI-antigen content increased continously. The active PAI form can be separated from the inactive form by gel-filtration. The inactive form behaves as a low Mr (about 50,000) component in the absence and in the presence of sodium dodecyl-sulphate. In contrast, the ac

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Stachowiak, Jeanne C., Erin E. Shugard, Pamela Caton, Bruce P. Mosier, Ron Renzi, Rafael V. Davalos, Gregory J. McGraw, Blake A. Simmons, Victoria A. Vandernoot, and Brent A. Haroldsen. "Automated Sample Preparation System for Rapid Biological Threat Detection." In ASME 2005 International Mechanical Engineering Congress and Exposition. ASMEDC, 2005. http://dx.doi.org/10.1115/imece2005-80945.

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Rapid, automated sample preparation of bacterial cells and spores is required for threat analysis by remotely deployed chemical and biological warning systems. Sandia is designing, building, and testing an automated front-end sample preparation system based on miniature and microfluidic components, with the goal of concentrating bacterial species collected from the air, harvesting and solubilizing proteins from them, and delivering them to Sandia’s MicroChemLab capillary gel electrophoresis system1,2 for analysis (Fig. 1). Miniature, motorized valves and pumps control flow between system compo

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Wiman, B., T. Carlsson, and J. Chmielewska. "EVIDENCE FOR A PLASMINOGEN ACTIVATOR INHIBITOR BINDING PROTEIN IN PLASMA." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642859.

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For several years it has been known that plasminogen activator inhibitor in plasma behaves as a high molecular weight compound on gelfiltration, in spite of that the molecular weight is only 50,000 in the presence of sodium dodecylsul-phate. The reason for this has so far been unknown. On gelfiltration of plasma, to which purified latent PAI from HT 1080 cells was added, the PAI antigen gel-filtered as a 50,000 Mr protein. However, if the latent form of PAI was reactivated by guanidinium chloride prior to the gel-filtra-tion experiment, an apparent molecular weight of about 250.000 for PAI ant

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Nurhidayat, I., S. Setiasih, S. Handayani, and S. Hudiyono. "Kinetic studies of bromelain purified from Palembang pineapple (Ananas comosus [L.] Merr) using gel filtration chromatography and its activity as antiplatelet aggregation." In PROCEEDINGS OF THE 3RD INTERNATIONAL SYMPOSIUM ON CURRENT PROGRESS IN MATHEMATICS AND SCIENCES 2017 (ISCPMS2017). Author(s), 2018. http://dx.doi.org/10.1063/1.5064065.

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Morinaga, T., Y. Itagaki, A. Suzuki, H. Yasuda, and K. Higashio. "PURIFICATION AND CHARACTERIZATION OF TISSUE PLASMINOGEN ACTIVATOR PRODUCED BY IMR-90 CELLS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644393.

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Tissue plasminogen activator ( t-PA ) produced by IMR-90 ( human embryonic fibroblast ) cells cultured in the serum-free medium ( DMEM ) containing 1% proteos^e peptone and 1.6 - 3.6mM CaCl2 was purified by the procedure consisted of ultrafiltration, immunoadsorpt ion chromatography, HPLC and lysine-Sepharose chromatography. The yield of t-PA from the culture broth was approximately 47%. The purified t-PA migrated as a single band on SDS-polyacrylamide gels. The molecular weight of the t-PA was estimated to be 66,000 by SDS-polyacrylamide gel electrophoresis and 69,000 by gel filtration method

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Masci, P. P., A. N. Whitaker, J. J. Morrison, and E. A. Bennett. "PURIFICATION AND CHARACTERIZATION OF THE PROCOAGULANT OF THE VENOM OF TROPIDECHIS CARINATUS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644322.

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Tropidechis carinatus is a venomous elapid snake distributed throughout Eastern Queensland. It has been considered as a tropical relative of Notechis scutatus and, similarly, the crude venom contains an indirect prothrombin activator, which will clot plasma provided that Factor V is present. Myotoxins and neurotoxins are also present. Envenomated patients regularly develop disseminated intravascular coagulation. The crude whole venom of T.carinatus was shown to have five major components by gel filtration, SDS PAGE and HPLC, and even more components by isoelectric focusing. The procoagulant el

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Gargan, P. E., and V. A. Ploplis. "IDENTIFICATION AND PURIFICATION OF AN INHIBITOR TO PLASMINOGEN ACTIVATORS FROM PROSTATE ADENOCARCINOMA CELLS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643192.

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The rat prostate adencarcinoma cell line (PA-III), derived from qerm-free Lobund Wistar rats has served as a unique model for prostatic cancer. The supernatant from confluent cultures of the cell line was shown by zymographic analysis to contain three molecular forms of plasminogen activator. One of the activators was characterized in a previous study and has been classified as a tissue type plasminogen activator(Mr=30,000). In the same study the activator with a Mr=45,000 was shown to be of the urokinase class. The high molecular weight(Mr=120, 000) activator, which has not been described pre

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Gheysen, D., L. Piérard, P. Jacobs, H. R. Lijnen, A. Bollen, and D. Collen. "PROPERTIES OF A HUMAN RECOMBINANT FUSION PROTEIN OF THE ‘FINGER’ DOMAIN OF TISSUE-TYPE PLASMINOGEN ACTIVATOR (t-PA) AND A TRUNCATED SINGLE CHAIN UROKINASE-TYPE PLASMINOGEN ACTIVATOR (scu-PA)." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643941.

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A hybrid between human tissue-type plasminogen activator (t-PA) and human single chain urokinase-type plasminogen activator (scu-PA) was obtained by ligation of cDNA fragments encoding the NH2-terminal amino acids 1 to 67 of t-PA and the COOH-ter-minal amino acids 136 to 411, of scu-PA. Both this chimaeric cDNA and cDNA encoding scu-PA were expressed in a mammalian system (HAK-cells) using bovine papilloma virus (BPV) derived vectors. Two stable cell lines were obtained which secreted the recombinant hybrid and the scu-PA at 1 μg/ml and 2 μg/ml u-PA related antigen respectively into the cultur

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Müller, E., A. Henschen, and G. Wunderer. "IDENTIFICATION OF A NEW HUMAN KININ, ILE-SER-BRADYKININ, BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND SEQUENCE ANALYSIS IN OVARIAN CARCINOMA ASCITES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642848.

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Human blood has been shown to contain two different kinin precursors, i.e0 the high and the low molecular mass kininogen0 These two kininogens release the same kinins, with the starting sequences Met-Lys-Arg-Pro-, Lys-Arg-Pro- or just Arg-Pro-depending on the releasing enzyme. The kinin starting with Arg-Pro- is denoted as bradykinin. In rats a different kininogen, called T-kininogen, is also present, especially as the major acute-phase protein in this species. The corresponding kinin, T-kinin, has the starting sequence Ile-Ser-Arg-Pro-. This type of kininogen or kinin has previously never bee

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