Academic literature on the topic 'GEME] Engineering Sciences'
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Journal articles on the topic "GEME] Engineering Sciences"
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Six, J. M. "Hidden gems [science and technology museums]." IEEE Spectrum 42, no. 1 (January 2005): 70–71. http://dx.doi.org/10.1109/mspec.2005.1377881.
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Ningtyas, Anindita Galuh Sekar, and Kadek Linda Kusnita. "Mengembangkan Wisata Desa Wongaya Gede Melalui Sosial Media." Jurnal Mandala Pengabdian Masyarakat 4, no. 1 (June 30, 2023): 171–74. http://dx.doi.org/10.35311/jmpm.v4i1.195.
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Desa Wisata khususnya Desa Wongaya Gede yang tergolong masih banyak yang belum mengetahui tentang wisatanya maka dari itu, diperlukan nya sosial media untuk mempublikasikan hal apa saja yang ada di sekitar Desa Wongaya Gede khususnya wisata dilakukan dengan cara membranding video melalui sosial media. adanya wisata lokal Desa Wongaya Gede sebagai rekomendasi dalam wisata desa melalui sosial media. Masa kini desa wisata kurang begitu diminati masyarakat luas, hal ini dikarenakan promosi desa wisata yang belum optimal. Desa Wongaya Gede merupakan salah satu Desa Wisata di Bali yang kurang begitu akrab di telinga masyarakat. Maka dari itu diperlukan untuk mempublikasikan Desa Wongaya Gede guna membantu sektor pariwisata. Maka dari itu dilakukan pemberian informasi kepada Kepala Daerah/ Ketua Wilayah tentang pentingnya sosial media untuk mempublikasikan Desa Wongaya Gede guna membangun sektor pariwisata. Dengan adanya sosial media diharapkan masyarakat dibantu dengan ketua wilayah setempat dapat berperan aktif untuk mempromosikan dan memajukan Desa Wongaya Gede
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Ribeiro, Vitor Hugo Alves, Beatriz Vanderlei Ribeiro, Cleidson Manoel Gomes da Silva, Carlos Frederico Martins, Cátia Oliveira Guimarães Abud, and Lucas Jacomini Abud. "Avaliação da qualidade do sêmen bovino criopreservado com diluidores de origem animal e vegetal." Brazilian Journal of Development 8, no. 10 (October 6, 2022): 66182–90. http://dx.doi.org/10.34117/bjdv8n10-091.
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A comercialização de sêmen bovino criopreservado é realizada em larga escala mundialmente. Entretanto, a utilização de diluidores com produtos de origem animal tem limitado a exportação de sêmen para outros países devido a possíveis riscos sanitários. Portanto, o objetivo deste estudo foi avaliar o efeito da substituição de diluidores contendo componentes de origem de animal por diluidor à base de lecitina de soja na criopreservação de sêmen bovino. O sêmen foi coletado por eletroejaculação a partir de seis touros Nelore com fertilidade comprovada. Após determinação da concentração espermática, as amostras de sêmen foram adicionadas em diferentes diluidores: diluidor comercial (Controle), à base de gema de ovo (TRIS-gema) e lecitina de soja (LS). Após o descongelamento, a cinética espermática foi avaliada pelo sistema CASA e a integridade de membrana citoplasmática e acrossomal foram avaliadas por epifluorescência. Não foi observada diferença significativa entre os diluidores TRIS-gema e LS para todos os parâmetros de cinética espermática avaliados (P>0,05). A avaliação de integridade de membrana e acrossoma após o descongelamento revelou taxas de viabilidade espermática significativamente superior (P<0,05) ao utilizar o diluidor comercial (30,80±10,0). No entanto, não foi observada diferença significativa (P>0,05) entre os diluidores contendo TRIS-gema (18,00±7,1) e LS (14,60±6,5). Em conclusão, a criopreservação de sêmen bovino com diluidor à base de LS apresentou ação crioprotetora similar ao diluidor convencional TRIS-gema. Portanto, a lecitina de soja é uma alternativa que pode ser utilizada com sucesso na criopreservação de sêmen bovino.
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Demming, Anna. "Gems in nanoscience." Nanotechnology 22, no. 17 (March 16, 2011): 170201. http://dx.doi.org/10.1088/0957-4484/22/17/170201.
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Setiawan, Aditya Sugih, and Rima Pratiwi Batubara. "Penerapan Prinsip Ekowisata di Situ Gede sebagai Daya Tarik Wisata Unggulan Kota Bogor." Altasia Jurnal Pariwisata Indonesia 4, no. 2 (August 12, 2022): 45. http://dx.doi.org/10.37253/altasia.v4i2.6758.
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Situ Gede merupakan salah satu kawasan wisata alam yang ada di kota Bogor. Keunggulan dari Kawasan Situ Gede yaitu disekitarnya terdapat hutan penelitian, darmaga. Situ Gede diharapkan dapat menjadi ruang publik yang dapat menjadi destinasi wisata terbaik bagi Jawa Barat. Metode penelitian ini menggunakan penelitian kualitatif dengan pendekatan deskriptif analitis. Penelitian dilakukan dengan observasi, wawancara dan penelusuran literatur. Adapun analisa data yang digunakan yaitu analisa data kualitatif berupa reduksi data, penyajian data, penarikan kesimpulan atau verifikasi. Prinsip berbasis alam dapat diterapkan dengan mengemas wisata edukasi. Prinsip ekologis berkelanjutan didekati dari optimalisasi fisik, sumber daya manusia, biaya dan manfaat. Prinsip edukatif lingkungan diterapkan kepada pengelola, masyarakat dan pengunjung. Prinsip berbasis masyarakat lokal, dilakukan dengan keterbukaan terhadap lowongan pekerjaan, pembuatan kebijakan dan pelatihan seni budaya. Prinsip berbasis ekowisata didekati dengan menyediakan program dan fasilitas pariwisata.
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Mufidah, Annisa'ul, Yuliati Sari, and Bayu Widiyanto. "Analisis Pembiasaan Harian Terhadap Pembentukan Karakter Peserta Didik." Bidayatuna Jurnal Pendidikan Guru Mandrasah Ibtidaiyah 6, no. 1 (April 6, 2023): 1–14. http://dx.doi.org/10.54471/bidayatuna.v6i1.2249.
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Today the lives of Indonesian citizens are experiencing moral degradation, especially among students, this requires the holding of character education. Schools are required to be able to carry out their roles and responsibilities to instill and develop good character values and help students shape and build their character with good values Teachers need to design learning, identify children's level of knowledge, motivate children and make activities learning fun. This research is a type of qualitative research. The approach used is descriptive qualitative to analyze the habituation carried out at SDN Tegal Gede 01. The purpose of this study is to analyze the daily habituation carried out at SDN Tegal Gede 01 towards the formation of students' character. Based on the results of observations and interviews, researchers can conclude that the habits that are carried out at SDN Tegal Gede 01 in developing the character of students are through daily routine activities, spontaneous activities and exemplary activities. Habituation activities carried out in schools can foster a positive attitude in students. Character education not only has a positive impact on students, character education through this habituation also improves the teacher's character or personality in developing core ethical and aesthetic values such as caring, honesty, fairness, responsibility, and respect for self and others
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Sommer, Laura Reisdörfer, Patrícia Graosque Ulguim Züge, Dianini Brum Frölech, Jéssica Gonsalez Cruz, Andrio Spiller Copatti, Flávia Saraiva Loy, Bruna Andressa dos Santos Oliveira, Jordana Caroline Nagel, Adriane Marinho de Assis, and Márcia Wulff Schuch. "Fenologia de amoreira-preta cultivada em recipientes com diferentes substratos." Research, Society and Development 11, no. 12 (September 20, 2022): e452111234948. http://dx.doi.org/10.33448/rsd-v11i12.34948.
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O objetivo do trabalho foi avaliar a duração, em dias, dos estádios fenológicos da amoreira-preta ‘Xavante’ cultivada em diferentes volumes de recipientes e substratos. Foi avaliada a duração (em dias) dos seguintes estádios fenológicos: início da brotação da gema florífera, início da floração, início da maturação do fruto, início da colheita de frutos. Também foi identificada uma gema dormente por haste, para avaliações da fenologia de frutos e observada a duração (em dias) dos seguintes estádios de desenvolvimento de frutos: gema dormente, gema brotando, botão de flor, flor parcialmente aberta, flor aberta, baga verde, baga parcialmente rosada, baga cor-de-rosa e baga madura. O ciclo da amoreira-preta ‘Xavante’ desde o início da brotação das gemas até o início da colheita variou entre 52 e 56 dias nos recipientes de volumes 10, 20 e 30 litros e nos substratos fibra de coco e casca de arroz carbonizada. O tempo decorrido entre os estádios de botão de flor e baga madura, foi semelhante nos recipientes de 10 e 30 litros. Porém, no volume de 20 litros, a média de dias entre os estádios foi menor. Conclui-se que a cultivar de amoreira-preta ‘Xavante’ apresenta boa adaptabilidade ao substrato fibra de coco independente do volume de recipiente. Os estádios fenológicos apresentaram, em média, o mesmo número de dias, independente do substrato e do recipiente observado.
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Yassin, Yahya H., Magnus Jahre, Per Gunnar Kjeldsberg, Snorre Aunet, and Francky Catthoor. "Fast and Accurate Edge Computing Energy Modeling and DVFS Implementation in GEM5 Using System Call Emulation Mode." Journal of Signal Processing Systems 93, no. 1 (May 29, 2020): 33–48. http://dx.doi.org/10.1007/s11265-020-01544-z.
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AbstractStringent power budgets in battery powered platforms have led to the development of energy saving techniques such as Dynamic Voltage and Frequency scaling (DVFS). For embedded system designers to be able to ripe the benefits of these techniques, support for efficient design space exploration must be available in system level simulators. The advent of the edge computing paradigm, with power constraints in the mW domain, has rendered this even more essential. Without a fast and accurate methodology for architecture simulation and energy estimation, the benefit of new ideas and solutions cannot be evaluated. In this paper, we propose a non-intrusive application controlled DVFS management implementation in the GEM5 simulator, used with GEM5’s system call emulation mode. We also propose a novel architecture independent energy model based on categorization of different measurable workload classes. Our energy model is parametrized and calibrated with power measurements on a SAM4L microcontroller board, containing an ARM Cortex M4 processor. Together with the GEM5 output statistics, the model accurately estimates the total energy consumption of our simulated system. The results from our modified GEM5 simulator are validated with representative signal processing applications. After correction of systematic offset errors, our results deviate with less than 4% compared to measurements from the SAM4L microcontroller. Our contributions in this paper can easily be tailored to other processor models in GEM5 and to future versions of GEM5. It will therefore enable system architects to explore new techniques and compare the improvements relative to existing architectures.
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Da Silva, Ivan do Nascimento, Manoella Evelyn Santos Lopes, Rebeca Bomfim de Araújo De Almeida, Juliana Arôxa Pereira Barbosa, Rodrigo Freitas Monte Bispo, Fabiano Timbó Barbosa, and Célio Fernando de Sousa Rodrigues. "Efeito de uma dieta hiperlipídica sobre a morfologia óssea." Brazilian Journal of Health Review 6, no. 5 (October 20, 2023): 25478–88. http://dx.doi.org/10.34119/bjhrv6n5-385.
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A hiperlipidemia é representada por concentração de triglicerídeos, de colesterol total e LDL aumentadas e redução dos níveis de colesterol HDL na corrente sanguínea. Essa alteração está relacionada a várias doenças, por exemplo a aterosclerose e osteoporose. Determinar o perfil histopatológico e clinico dos casos de alteração óssea relacionados a hipercolesterolemia em coelhos que foram submetidos a uma dieta hiperlipídica. O estudo é do tipo experimental. Onde foram coletadas as amostras de 18 animais, coelhos albinos Nova Zelândia, que foram randomizados em dois grupos: G1 – grupo controle (apenas ração) e G2 (ração mais gema). Seguidamente à eutanásia foi removido parte da tíbia e fíbula, a partir de uma dissecção da face posterior da pata posterior esquerda. Com as amostras em mãos deu-se início a etapa de preparação das lâminas para análise histológica com fragmentos ósseos, seguindo protocolos de rotina laboratorial. No grupo que se alimentou paneas de ração há um maior numero de lceulas hematopoieticas, já no grupo alimentado com gema de ovo há uma diminuição no número de trabéculas, aumento no espaçamento trabecular, maior número de células adiposas e menor número de células hematopoieticas. Foi constatado notáveis alterações morfológicas na histopatologia das lâminas analisadas, apresentando aumento de células adiposas e diminuição de células hematopoiéticas no grupo alimentado com ração e gema (G2) comparando com o grupo controle (G1), alimentado exclusivamente com ração.
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Macêdo, Laércio Fontinele Bandeira de, Letícia Soares de Araújo Teixeira, Wcleuden Matias Nascimento, Clarissa de Castro e. Braga, Kenney de Paiva Porfírio, Francisca Kelly dos Santos Silva, Sara Camila da Silveira Costa, et al. "Avaliação da viabilidade espermática de sêmen caprino criopreservado em meio ACP-101c e TRIS acrescido de gema de ovo de Numida meleagris." Research, Society and Development 10, no. 14 (November 1, 2021): e265101422064. http://dx.doi.org/10.33448/rsd-v10i14.22064.
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Um meio diluente ideal para a criopreservação seminal deve suprir as células espermáticas com energia, proteção e manutenção de um ambiente adequado à sua sobrevivência. Objetivou-se avaliar a integridade in vitro do sêmen caprino criopreservado em meio ACP-101c® associado à gema de ovo de Numida meleagris, por meio de duas técnicas de análise individual da célula espermática. Foram colhidos 15 ejaculados de cinco caprinos, com auxílio de uma vagina artificial. Os ejaculados foram reunidos em um pool, e dividido em 12 grupos, sendo dois grupos controles: GC1 TRIS, com adição 2,5% da gema de ovo de Gallus gallus domesticus GOGD, GC2 ACP-101c®, com adição 2,5% da gema de ovo de Gallus gallus domesticus GOGD e dez grupos experimentais GE, contendo a adição da gema de ovo de Numida meleagris. Seguidamente, as alíquotas foram envasadas em palhetas francesas de 0,25 mL e congeladas com auxílio do aparelho TK3000® e armazenadas em nitrogênio líquido. Amostras foram descongeladas após sete dias e avaliadas quanto à integridade do DNA e quanto a sua ultraestrutura individual, por meio do teste cometa e microscopia eletrônica de transmissão, respectivamente. Nos resultados obtidos pelo teste cometa, não foi evidenciado diferença estatística quanto ao comprimento da cauda, entre os grupos TRIS acrescido de GONM, nas concentrações de 2,5%, 5% e 10% em relação ao grupo controle TRIS 2,5% de GOGD. Também não houve diferença estatística quanto ao percentual de fragmentação de DNA na cauda, nos grupos TRIS com 2,5%; 5%; e 20% de GONM em comparação ao grupo controle TRIS 2,5% GOGD (P>0,05). Os grupos ACP® com 10%, 15% e 20% de GONM apresentaram maior comprimento de cauda quando comparados aos grupos ACP com 2,5% e 5% de GONM e grupo controle (ACP® 2,5% de GOGD). Na análise ultraestrutural o grupo ACP® com 10% de GONM, destacou-se com melhor integridade celular frente aos demais grupos, inclusive, frente às amostras avaliadas do grupo controle. Dessa forma, conclui-se que a gema de ovo de Numida meleagris, como crioprotetor externo de membrana, acrescida aos diluentes ACP-101c® ou TRIS, pode reduzir os danos causados durante o processo de criopreservação do sêmen caprino.
Dissertations / Theses on the topic "GEME] Engineering Sciences"
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Gustafsson, Mika. "Gene networks from high-throughput data : Reverse engineering and analysis." Doctoral thesis, Linköpings universitet, Kommunikations- och transportsystem, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-54089.
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Experimental innovations starting in the 1990’s leading to the advent of high-throughput experiments in cellular biology have made it possible to measure thousands of genes simultaneously at a modest cost. This enables the discovery of new unexpected relationships between genes in addition to the possibility of falsify existing. To benefit as much as possible from these experiments the new inter disciplinary research field of systems biology have materialized. Systems biology goes beyond the conventional reductionist approach and aims at learning the whole system under the assumption that the system is greater than the sum of its parts. One emerging enterprise in systems biology is to use the high-throughput data to reverse engineer the web of gene regulatory interactions governing the cellular dynamics. This relatively new endeavor goes further than clustering genes with similar expression patterns and requires the separation of cause of gene expression from the effect. Despite the rapid data increase we then face the problem of having too few experiments to determine which regulations are active as the number of putative interactions has increased dramatic as the number of units in the system has increased. One possibility to overcome this problem is to impose more biologically motivated constraints. However, what is a biological fact or not is often not obvious and may be condition dependent. Moreover, investigations have suggested several statistical facts about gene regulatory networks, which motivate the development of new reverse engineering algorithms, relying on different model assumptions. As a result numerous new reverse engineering algorithms for gene regulatory networks has been proposed. As a consequent, there has grown an interest in the community to assess the performance of different attempts in fair trials on “real” biological problems. This resulted in the annually held DREAM conference which contains computational challenges that can be solved by the prosing researchers directly, and are evaluated by the chairs of the conference after the submission deadline. This thesis contains the evolution of regularization schemes to reverse engineer gene networks from high-throughput data within the framework of ordinary differential equations. Furthermore, to understand gene networks a substantial part of it also concerns statistical analysis of gene networks. First, we reverse engineer a genome-wide regulatory network based solely on microarray data utilizing an extremely simple strategy assuming sparseness (LASSO). To validate and analyze this network we also develop some statistical tools. Then we present a refinement of the initial strategy which is the algorithm for which we achieved best performer at the DREAM2 conference. This strategy is further refined into a reverse engineering scheme which also can include external high-throughput data, which we confirm to be of relevance as we achieved best performer in the DREAM3 conference as well. Finally, the tools we developed to analyze stability and flexibility in linearized ordinary differential equations representing gene regulatory networks is further discussed.
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Bolliet, Catherine. "Gene-supplemented collagen scaffolds for non-viral gene delivery for brain tissue engineering." Thesis, Massachusetts Institute of Technology, 2007. http://hdl.handle.net/1721.1/38586.
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Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2007.Includes bibliographical references (p. 90-95).
Recent advances in tissue engineering, combining an extracellular matrix (ECM)-like vehicle with therapeutic molecules, cells and/or genes has yielded promising results for brain injury repair. The purpose of this thesis was to develop a collagen scaffold for the non-viral delivery of the gene encoding for Glial Cell-Derived Neurotrophic Factor (GDNF); hence to provide a local, long-term release and overexpression of GDNF via transfection of cells seeded into the scaffold or endogenous cells. The first part of the thesis aimed to investigate the in vitro transfection of marrow stromal stem cells (also referred to as mesenchymal stem cells, MSCs) in monolayer with plasmid GDNF ([mu]gDNF). Several parameters were evaluated: the choice of a transfer reagent (GenePorter2 versus Lipofectamine 2000), the doses of plasmid incorporated in the liposomes (ranging from 0.2[mu]g to 2[mu]g), the post-transfection medium (Medium 1: DMEM low glucose, 20% FBS and 1% antibiotic versus Medium 2: DMEM low glucose, 20% FBS, 1% antibiotic and 10ng/ml FGF-2) and the culture environment during transfection (static versus dynamic). The objective of the second part was to determine the conditions, including the design of the scaffold and the method of seeding, under which MSCs could attach and grow on the scaffold.
(cont.) Collagen scaffolds were made by a freeze-drying technique and prepared with various amounts of collagen, cross-link densities, and freezing temperatures. The effect of gene supplementation on the cross-link density was evaluated using the swelling ratio. Finally, the aim of the third part was to evaluate different parameters to optimize the transfection of cells grown in the scaffolds. The profile of production of GDNF was studied for different cross-link density, initial plasmid dose (2 and 10 [mu]g) and plasmid-transfection reagent ratio. Finally the effect of the pore diameter and static and dynamic culture environments were tested to optimize the in vitro conditions for the plasmid uptake and expression by the MSCs. The results demonstrated the possibility of using non-viral transfection conditions in vitro to enable MSCs to express a selected neurotrophic factor, GDNF, in therapeutic doses. MSCs were shown to over-express GDNF for at least a two-week period of time. Lipoplexes loaded with as little as 0.2 [mu]g could result in a significant production of GDNF by MSCs for several days, before falling off to control levels after one week. For the highest loading of plasmid (2 [mu]g), the level of GDNF production was still above the control after 2 weeks.
(cont.) Dynamic transfection had a dramatic effect on the production of GDNF. The accumulated amount of GDNF during the 2-week period reached 65 ng/ml compared to 20 ng/ml produced in static conditions. The growth factor bFGF, which is used in transdifferentiation of MSCs for a neuronal phenotype, was shown to promote a high level of cell death when used in the post-transfection medium. Collagen scaffolds can be prepared to incorporate the plasmid DNA-lipid complexes for subsequent release. Also, gene and subsequent cross-link density have an effect on the mechanical behavior of scaffolds. Finally, the gene-supplemented collagen scaffolds can serve as a carrier for lipoplexes and modified MSCs and provide a long-term overexpression of GDNF. The level of gene expression in the collagen constructs was lower than those obtained in MSC monolayers but high enough to result in therapeutic doses previously found in vitro. The cross-linking treatment did not affect significantly the release profile of GDNF. The application of orbital shaking during the 4 hours of transfection had a positive effect on the production of GDNF but not as strong as reported in monolayer studies. The load of plasmid DNA is a prominent parameter in the three-dimensional (3-D) transfection.
(cont.) In this study, the highest level of GDNF expression was observed for 10 [mu]g of plasmid DNA and 6 days after transfection. Overall, these results demonstrated that the combination of tissue engineering and non-viral transfection of MSCs for the over-expression of GDNF was a promising approach for the long-term production of selected neurotrophic growth factors. This approach could provide benefits in the treatment of conditions involving the loss of brain tissue.
by Catherine Bolliet.
S.M.
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Capito, Ramille M. (Ramille Marie). "Gene-supplemented collagen-glycosaminoglycan scaffolds for nonviral gene delivery in articular cartilage tissue engineering." Thesis, Massachusetts Institute of Technology, 2006. http://hdl.handle.net/1721.1/36203.
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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2006.Includes bibliographical references.
Three-dimensional scaffolds and growth factors have been shown to be important for articular cartilage tissue engineering. A major problem in using recombinant proteins in vivo, however, is the inability to maintain therapeutic levels over prolonged times due to degradation or diffusion from the defect site. The goal of this thesis was to develop a method to employ type II collagen-glycosaminoglycan (CG) scaffolds for the nonviral delivery of the gene encoding for insulin-like growth factor (IGF)-I, as a novel means to provide a local, elevated, and prolonged release of a therapeutic growth factor via transfection of cells seeded or migrating within the scaffold. In vitro studies were performed to evaluate gene-supplemented CG (GSCG) scaffolds, including: 1) the type of expansion medium to use for growing chondrocytes prior to transfection, 2) methods of incorporating genes within scaffolds, 3) additional incorporation of transfection enhancers, and 4) the use of mesenchymal stem cells (MSCs) as an alternative cell source for articular cartilage tissue engineering.
(cont.) The medium used during monolayer expansion not only had a significant effect on subsequent biosynthesis and chondrogenesis in CG scaffolds, but also on gene transfer to chondrocyte monolayers. The expansion medium that resulted in enhanced 3-D biosynthesis and gene transfer to cells in monolayer was used throughout the rest of the studies. Greater plasmid retention within GSCG scaffolds was achieved by chemically cross-linking the plasmid IGF-1 (pIGF-1) to the scaffold (compared to simple plasmid absorption), and resulted in more steady and prolonged IGF-1 overexpression by seeded chondrocytes. Incorporation of a lipid transfection reagent or gelatin nanoparticles encapsulating pIGF-1 significantly enhanced gene expression. The method of gene incorporation and the type of transfection enhancer were important variables that controlled the initiation, amount, and duration of growth factor release. IGF-1 overexpression by cells successfully transfected within GSCG scaffolds also increased biosynthesis of cartilage matrix molecules and chondrogenesis. Finally, MSCs seeded into GSCG scaffolds were able to be successfully transfected and maintained IGF-1 overexpression for at least 2 weeks post-seeding.
(cont.) These findings show promise in using GSCG scaffolds for providing a local, prolonged, and therapeutic release of desired growth factors using nonviral transfection methods for tissue engineering or regenerative medicine applications.
by Ramille M. Capito.
Ph.D.
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St, John Oliver Tudor Lockhart. "Genome engineering and gene drive in the mosquito aedes aegypti." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:1251080e-cf7b-4bdd-b01e-d01748ead2d2.
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Genetic control strategies are a novel method for reducing populations of pest insects such as the yellow fever mosquito Aedes aegypti, a major vector of several important arboviral diseases. This thesis describes efforts to develop new tools to engineer the Ae. aegypti genome and to better understand existing tools, and furthermore to use these to engineer a gene drive system in Ae. aegypti. The piggyBac transposon was found to be extremely stable in the germline of Ae. aegypti, and transposons engineered into the germline could not be remobilized with either an endogenous or exogenous source of piggyBac transposase. Conversely, somatic remobilization of piggyBac transposons was found to be readily detectable in the presence of a source of active transposase, the first report of such remobilization in Ae. aegypti. Toward new tools for genome engineering, the site-specific integrase from the phage φC31 was successfully used to promote exchange between a transgene cassette inserted into the genome of Ae. aegypti and a cassette in a plasmid vector, in the first demonstration of recombinase mediated cassette exchange technology in a pest insect species. The integrases from phages φRV1 and Bxb1 were not found to be active in the germline of the mosquito. Finally, development of a gene drive system in Ae. aegypti using an RNAi-mediated killer-rescue mechanism was attempted. Tissue-specific expression of tTAV-regulated-toxic effectors genes, using the promoter regions of the blood meal induced genes Carboxypeptidase A-1, 30Kb and Vitellogenin A, was possible, but sex-specificity was not achieved. A blood meal inducible lethal phenotype was not possible using the chosen promoters, with expression of the effectors either leading to death in early development or to a sublethal phenotype. RNAi against tTAV fused to the Mnp fragment of the dengue virus’ genome was tissue specific, but was found to be highly effective in the fat body suggesting that the Vitellogenin A was the best candidate for the engineering of killer-rescue systems in the mosquito.
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Davies, Emma L. "Somatic cell gene therapy for diabetes mellitus : engineering a surrogate B-cell." Thesis, Aston University, 1996. http://publications.aston.ac.uk/10940/.
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Improved methods of insulin delivery are required for the treatment of insulin-dependent diabetes mellitus (IDDM) to achieve a more physiological profile of glucose homeostasis. Somatic cell gene therapy offers the prospect that insulin could be delivered by an autologous cell implant, engineered to secrete insulin in response to glucose. This study explores the feasibility of manipulating somatic cells to behave as a surrogate insulin-secreting β-cells. Initial studies were conducted using mouse pituitary AtT20 cells as a model, since these cells possess an endogenous complement of enzymes capable of processing proinsulin to mature insulin. Glucose sensitive insulin secretion was conferred to these cells by transfection with plasmids containing the human preproinsulin gene (hppI-1) and the GLUT2 gene for the glucose transporter isoform 2. Insulin secretion was responsive to changes in the glucose concentration up to about 50μM. Further studies to up-rate this glucose sensitivity into the mM range will require manipulation of the hexokinase and glucokinase enzymes. Intraperitoneal implantation of the manipulated AtT20 cells into athymic nude mice with streptozotocin-induced diabetes resulted in decreased plasma glucose concentrations. The cells formed vascularised tumours in vivo which were shown to contain insulin-secreting cells. To achieve proinsulin processing in non-endocrine cells, co-transfection with a suitable enzyme, or mutagenesis of the proinsulin itself are necessary. The mutation of the human preproinsulin gene to the consensus sequence for cleavage by the subtilisin-like serine protease, furin, was carried out. Co-transfection of human fibroblasts with wild-type proinsulin and furin resulted in 58% conversion to mature insulin by these cells. Intraperitoneal implantation of the mature-insulin secreting human fibroblasts into the diabetic nude mouse animal model gave less encouraging results than the AtT20 cells, apparently due to poor vascularisation. Cell aggregations removed from the mice at autopsy were shown to contain insulin secreting cells only at the periphery. This thesis provides evidence that it is possible to construct, by cellular engineering, a glucose-sensitive insulin-secreting surrogate β-cell. Therefore, somatic cell gene therapy offers a feasible alternative for insulin delivery in IDDM patients.
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Zhao, Jia. "Engineering serine integrase-based synthetic gene circuits for cellular memory and counting." Thesis, University of Glasgow, 2015. http://theses.gla.ac.uk/6911/.
Full textAbstract:
A cellular counting system based on synthetic gene circuits would enable complex biological programming and be used in many biotechnology applications. Although a variety of synthetic memory circuits have been constructed, basic modules that can be assembled into a counting system are lacking. This thesis focuses on engineering a binary counting module, which can alternate between two states in response to a single repeating input signal. The highly directional large serine bacteriophage integrases were utilised as the basis for the synthetic circuits constructed in this study. Integrases and their protein co-factors, the recombination directionality factor (RDF) can change the orientation of a specific DNA segment flanked by two recombination sites. Integrase alone switches the orientation in one direction, and this directionality is reversed by the addition of its corresponding RDF. The two orientations can be used to turn gene expression on and off, leading to distinct output states which can be thought of as representing a single binary digit (0 and 1) heritably stored in the DNA. In this study, three different serine integrase-based synthetic gene circuits for cellular memory and counting were engineered and characterised. A set-reset latch was first constructed. By expressing ϕC31 integrase and co-expressing integrase with RDF Gp3 from two independent inducible systems, the orientation of the invertible DNA in the set-reset latch was inverted and restored respectively. This device demonstrated that ϕC31 integrase can successfully encode information into plasmid DNA. Next, a state-based latch was constructed, in which the gp3 gene was placed inside the invertible DNA segment to couple its transcriptional regulation to the circuit state. Integrase expression triggered by one input signal resulted in inversion of the invertible DNA, placing the gp3 gene in the correct orientation for transcription. Gp3 expression can then be triggered by another input signal to reverse the directionality of integrase, restoring the DNA back to its original configuration. By optimising the stoichiometry and kinetics of integrase and Gp3 expression, efficient switching of both multi-copy plasmid and single copy chromosomal DNA was achieved. Finally, the state-based latch was developed into a binary counting module by introducing a delay mechanism, in which gp3 transcription was inhibited by a state-based repressor during recombination requiring the absence of Gp3. Placing expression of gp3 under the control of the invertible DNA, allowed a single input signal controlling only integrase expression to switch the module between OFF (0) and ON (1). This is the first integrase-based module that generates different outputs in response to the same input signal and a fundamental step towards building a genetic binary counter with large counting capacity.
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Legault-Coutu, Daniel. "Studies on mesenchymal stem cells: In vivo identity, cellular biochemistry and use in gene therapy and tissue engineering." Thesis, McGill University, 2011. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=103509.
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In 1868, the French experimental physiologist Goujon demonstrated the transplantability and osteogenic potential of bone marrow. More than a hundred years later, Friedenstein first isolated the cells responsible for this osteogenic capacity and demonstrated the existence of a non-hematopoietic stem cell within bone marrow. Since then, the field of mesenchymal stem cell (MSC) research has generated an incredible amount of data aimed at characterizing these cells and indentifying their true in vivo identity and localization, but also exploring their therapeutic potential in animal models. These studies served as a basis for more than 100 human clinical trials using MSCs in the last decade. However, many questions remain unanswered about MSCs. Current research thus aims at improving our understanding of these intriguing cells in order to better use them therapeutically. Four main themes can be identified in MSC research: 1) Characterization and identification of MSCs subsets, 2) Elucidation of the development origin and in vivo identity of MSCs, 3) Identification of the cellular and molecular mechanisms underlying the therapeutic effects of MSCs, and 4) pre-clinical (translational) and clinical use of MSCs. In the manuscript-based thesis presented here, I will present three original research articles covering all four of these themes. In chapter 2, I describe the use of novel cell surface markers to identify MSCs in vivo and in vitro. I initially show that FGF-receptors (FGFRs) are developmentally-regulated in both bone tissues and MSCs. Using these markers, I identified different MSCs subsets in bone tissues including perichondrium, periosteum and trabecular bone. Some of these cells appeared as pericytes in periosteum and trabecular marrow, supporting the model of a perichondrial MSC upstream of a pericytic osteo-stromal progenitor. Finally, I found that FGFRs activation leads to self-renewing proliferation of MSCs in vitro by reversibly inhibiting cellular senescence, providing a biological relevance for the expression of these markers. In chapter 3, I present the preliminary biochemical characterization of periostin, a poorly characterized matricellular protein abundantly expressed by MSCs in vitro and in vivo. I identify a post-translational modification in periostin that will undoubtedly help uncover its roles in MSCs (fate decision, proliferation, migration), in hematopoietic support, and in tissue repair. Finally, in chapter 4 I present our efforts to use MSCs in a cell-based gene therapy approach for hemophilia B. I show that the successful transplantation, engraftment, survival, differentiation, self-renewal and protein delivery by MSCs requires complex tissue engineering techniques and hierarchical scaffold design. More specifically, three-dimensional biomaterials scaffolds required optimization at the nano-, micro- and macroscale in order to sustain long term engraftment and protein delivery by MSCs in a murine model of hemophilia B. Taken together, the findings presented here provide significant advances in understanding MSCs, both in their fundamental biology and therapeutic potential.En 1868, le physiologiste expérimental Goujon démontre que la moelle osseuse peut se transplanter et possède des propriétés ostéogéniques. Près de cent ans plus tard, Friedenstein réussi à isoler les cellules de la moelle responsables de ces propriétés et démontre ainsi l'existence d'une cellule souche non-hématopoïétique résidant dans la moelle osseuse. Depuis, la recherche sur les cellules souches mésenchymateuses (CSM) a généré un nombre considérable d'articles évaluant les caractéristiques in vitro de ces cellules, leur expression de marqueurs de surface, essayant de définir leur identité et leur localisation in vivo, mais aussi testant leurs propriétés thérapeutiques chez les animaux. Ces recherches servirent de base à plus de 100 études cliniques chez l'humain enregistrées jusqu'à maintenant, utilisant les CSM pour traiter diverses maladies. Cependant, plusieurs questions restent non résolues concernant ces intrigantes cellules souches. C'est pourquoi la recherche actuelle sur les CSM tente de répondre à ces questions fondamentales, de manière à pouvoir mieux comprendre les CSM et ainsi à mieux les utiliser thérapeutiquement. On note quatre thèmes majeurs dans la recherche sur les CSM actuelle : 1) la caractérisation in vitro des CSM et l'identification de nouveaux marqueurs de surface, 2) la recherche de l'identité in vivo des CSM et de leur niche ou localisation, 3) l'élucidation des mécanismes cellulaires et moléculaires impliqués dans leurs propriétés thérapeutiques, et 4) la recherche préclinique et clinique utilisant les CSM pour traiter des maladies. Dans la thèse par manuscrits présentée ici, je présente trois articles de recherche couvrant l'ensemble de ces quatre thèmes. Au chapitre 2, j'identifie une famille de récepteurs membranaires qui est régulée de façon développementale dans les tissus osseux et dans les CSM : les récepteurs FGF. Je démontre que ces récepteurs peuvent être utilisés pour identifier les CSM dans différents compartiments osseux tels que le perichondrium, le periosteum et l'os trabéculaire, de manière à suggérer l'existence de CSM primitives dans le perichondrium. Nous verrons également comment l'activation des récepteurs FGF sur les CSM permet leur prolifération tout en inhibant leur sénescence, leur permettant ainsi de s'auto-renouveller. Au chapitre 3, je présente la caractérisation biochimique préliminaire de periostin, une protéine matricellulaire peu connue et abondamment produite par les CSM. J'identifie une modification post-traductionnelle sur periostin qui permettra de mieux comprendre ses divers rôles dans la différentiation des CSM, leur capacité de supporter l'hématopoïèse et de participer à la réparation des tissus endommagés. Finalement, au chapitre 4 je présente une étude visant à utiliser les CSM pour la thérapie génique de l'hémophilie B. Je démontre que la survie, la différentiation, l'auto-renouvellement et la production de protéines thérapeutiques par les CSM après transplantation nécessitent l'utilisation de techniques d'ingénierie tissulaire complexes. Plus spécifiquement, j'ai dû optimiser des biomatériaux 3D à l'échelle nano-, micro- et macroscopique pour permettre la survie et la production de protéine à long-terme par les CSM dans des souris hémophiles. En conclusion, les résultats présentés ici représentent plusieurs avancées significatives dans notre compréhension de la biologie fondamentale des CSM mais également de leurs propriétés thérapeutiques.
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Li, Yan 1978 July 15. "Gene expression array simulator." Thesis, Massachusetts Institute of Technology, 2002. http://hdl.handle.net/1721.1/87263.
Full textAbstract:
Thesis (M.Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, June 2002."May 10, 2002.
Includes bibliographical references (leaf 141).
by Yan Li.
M.Eng.
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Kodysh, Yuliya. "Using co-expression to redefine functional gene sets for gene set enrichment analysis." Thesis, Massachusetts Institute of Technology, 2007. http://hdl.handle.net/1721.1/41661.
Full textAbstract:
Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2007.Includes bibliographical references (p. 89-90).
Manually curated gene sets related to a biological function often contain genes that are not tightly co-regulated transcriptionally. which obscures the evidence of coordinated differential expression of these gene sets in relevant experiments. To address this problem, we explored strategies to refine the manually curated subcollection of the Molecular Signatures Database (MSigDB) for use with Gene Set Enrichment Analysis (GSEA). We examined the manually curated gene sets in context of an atlas of gene expression of many normal human tissues. To refine gene sets, we clustered the genes in each set based on co-expression across the tissues to produce more tightly co-regulated children gene sets that are also likely more accurate representations of the biological process or processes described by the gene set. We evaluated the performance of the clustering algorithms by refining gene sets in the context of several published GSEA analyses and verifying that the children gene sets score higher with GSEA than do the parents. We created and annotated a new, refined version of a large portion of the manually curated component of MSigDB, which we hope will be a resource for the GSEA community.
by Yuliya Kodysh.
M.Eng.
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Gyoergy, Andras. "Functional modularity in gene networks." Thesis, Massachusetts Institute of Technology, 2016. http://hdl.handle.net/1721.1/103726.
Full textAbstract:
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Electrical Engineering and Computer Science, 2016.Cataloged from PDF version of thesis.
Includes bibliographical references (pages 141-150).
This thesis addresses two sources of context-dependence in both systems and synthetic biology: retroactivity and competition for shared cellular resources. The contribution is the development of simple-to-use computational tools that aide the analysis and design of multi-module genetic systems. These tools are a result of combining mathematical modeling and theoretical analysis with experiments performed in Escherichia coli. While current approaches most often neglect to account for context-dependence in living systems, experimental evidence demonstrates that such effects have profound influence on system behavior. As a result, modules developed separately are likely to behave differently from predicted, so that they need to be redesigned through a lengthy and ad hoc process every time they are inserted into a different system. To overcome this major limitation, in this thesis I expand the description of gene circuits. First, the description of modules is appended by quantities similar to input and output impedance in electrical networks theory. Second, the description of each protein is appended by a quantity characterizing the amount of resources that are sequestered for its production. As a result, the behavior of modules upon interconnection becomes predictable, facilitating both the rational design of synthetic circuits and furthering our understanding of natural systems. Application examples are considered, which include the design of oscillators and toggle switches, network identification problems, and standard metabolic optimization problems, such as maximizing reaction rates catalyzed by multiple enzymes and maximizing the steady state concentration of heteromultimer complexes.
by Andras Gyoergy.
Ph. D.
Books on the topic "GEME] Engineering Sciences"
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C, Schaefer Brian, ed. Gene cloning and analysis: Current innovations. Norfolk, England: Horizon Scientific Press, 1997.
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Huard, Johnny. Gene Therapy and Tissue Engineering in Orthopaedic and Sports Medicine. Boston, MA: Birkhäuser Boston, 2000.
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A, Ceccarelli, and Wallace A. 1963-, eds. Genetic engineering. 2nd ed. Oxford: Bios, 2001.
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Alison, Thomson, and McWhir Jim, eds. Gene targeting and embryonic stem cells. London: Garland Science/BIOS Scientific, 2004.
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L, Beaudet Arthur, Mulligan Richard, and Verma Inder M, eds. Gene transfer and gene therapy: Proceedings of an E.I. du Pont de Nemours-UCLA Symposium, held at Tamarron, Colorado, February 6-12, 1988. New York: Liss, 1989.
Find full textBook chapters on the topic "GEME] Engineering Sciences"
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Gruchalla, Kenny, and Nicholas Brunhart-Lupo. "The Utility of Virtual Reality for Science and Engineering." In VR Developer Gems, 383–402. Boca Raton : Taylor & Francis, a CRC title, part of the Taylor & Francis imprint, a member of the Taylor & Francis Group, the academic division of T&F: A K Peters/CRC Press, 2019. http://dx.doi.org/10.1201/b21598-21.
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Chen, Tielong, and Xiaojie Xie. "MSCs as a Vector of Gene Engineering." In Advanced Topics in Science and Technology in China, 87–95. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-540-88150-6_6.
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Sarmah, Bidyut Kumar, Moloya Gohain, Basanta Kumar Borah, and Sumita Acharjee. "Cisgenesis: Engineering Plant Genome by Harnessing Compatible Gene Pools." In Concepts and Strategies in Plant Sciences, 193–216. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-63372-1_8.
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Swain, Martin, Johannes J. Mandel, and Werner Dubitzky. "Comparing Grid Computing Solutions for Reverse-Engineering Gene Regulatory Networks." In Computational Science – ICCS 2008, 106–15. Berlin, Heidelberg: Springer Berlin Heidelberg, 2008. http://dx.doi.org/10.1007/978-3-540-69384-0_16.
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Jin, Andrew, and Igor Linkov. "Synthetic Biology Brings New Challenges to Managing Biosecurity and Biosafety." In NATO Science for Peace and Security Series C: Environmental Security, 117–29. Dordrecht: Springer Netherlands, 2021. http://dx.doi.org/10.1007/978-94-024-2086-9_8.
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AbstractNovel biology technologies like gene editing and genetic engineering are creating a proliferation of breakthroughs in engineered biological systems that will change our world in areas ranging from medicine, to textiles, to energy. New developments in gene editing technologies, especially CRISPR-Cas9, have shown early signs of extraordinary potential in a variety of fields, including from basic research, applied biotechnology, and biomedical research. While the possibility of directly targeting and modifying genomic sequences in almost all eukaryotic cells could significantly improve standards of living, these technologies have the potential to pose serious biological hazards.
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Collins, James J. "Engineering Gene Regulatory Networks: A Reductionist Approach to Systems Biology." In Lecture Notes in Computer Science, 505. Berlin, Heidelberg: Springer Berlin Heidelberg, 2005. http://dx.doi.org/10.1007/11415770_38.
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Maraziotis, Ioannis, Andrei Dragomir, and Anastasios Bezerianos. "Recurrent Neuro-fuzzy Network Models for Reverse Engineering Gene Regulatory Interactions." In Lecture Notes in Computer Science, 24–34. Berlin, Heidelberg: Springer Berlin Heidelberg, 2005. http://dx.doi.org/10.1007/11560500_3.
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Kim, Jaeyoung, and Miyoung Shin. "A FC-GSEA Approach to Identify Significant Gene-Sets Using Microarray Gene Expression Data." In Advances in Computational Science and Engineering, 115–28. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-642-10238-7_10.
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Al-Turaiki, Israa M., Hassan Mathkour, Ameur Touir, and Saleh Hammami. "Computational Approaches for Gene Prediction: A Comparative Survey." In Informatics Engineering and Information Science, 14–25. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-25453-6_2.
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Gehl, Julie. "Electroporation for Medical Use in Drug and Gene Electrotransfer." In Advances in Electrochemical Sciences and Engineering, 369–88. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2012. http://dx.doi.org/10.1002/9783527644117.ch8.
Full textConference papers on the topic "GEME] Engineering Sciences"
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Bailey, Mike, Steve Cunningham, and Lew Hitchner. "Teaching gems for computer science and engineering." In ACM SIGGRAPH 2002 conference abstracts and applications. New York, New York, USA: ACM Press, 2002. http://dx.doi.org/10.1145/1242073.1242080.
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Ranieri, John P. "Tissue Engineering: A Review." In ASME 1996 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 1996. http://dx.doi.org/10.1115/imece1996-1162.
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Abstract Tissue engineering is defined as the in vitro or in vivo creation or regeneration of differentiated tissue for the clinical replacement of a compromised body structure. The ultimate goal of tissue engineering is to reconstruct an organ by taking advantage of recent progress in molecular biology (i.e., mechanisms controlling cell differentiation and gene transfer), materials science (development of “smart” and bioresorbable polymers), and surgical techniques. Tissue engineering is not so much a science in the traditional sense, but an amalgam of technologies from disparate fields that are grouped such that clinically relevant tissue replacement therapies may result.
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Loban, Liudmyla, Nina Pyliak, and Vladislav Yaroshevsky. "Gene resource of industrially important microbial culture collection for agriculture biologization." In Scientific International Symposium "Plant Protection – Achievements and Perspectives". Institute of Genetics, Physiology and Plant Protection, Republic of Moldova, 2023. http://dx.doi.org/10.53040/ppap2023.26.
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The article is dedicated to describing the gene resource of Industrially Important Microbial Culture Collection for Agriculture Biologization of Engineering and Technological Institute "Biotekhnika" of the National Academy of Agrarian Sciences of Ukraine, which holds the status of the Ukrainian National Asset. The Collection includes known industrial microbial strains with antagonistic, entomocidal, rodenticidal, nematocidal, and cellulosolytic properties. There are natural isolates that can transform insoluble phosphorus and ones are phytohormones producers in the Collection. The Collection can serve as a ground of experience exchange for scientists and specialists working in the field of plant protection, as well as for educating students’ biologists, biotechnologists, and agronomists.
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Simionescu, Bogdan C. "Polymer engineering focusing on drug/gene delivery and tissue engineering: from simple towards complex architectures and hybrid materials." In the 39th American Romanian Academy of Arts and Sciences Congress. ARA Publisher, 2015. http://dx.doi.org/10.14510/39ara2015.3902.
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Arotaritei, Dragos, and Cristian Rotariu. "E-LEARNING IN BIOMEDICAL ENGINEERING EDUCATION - A REVIEW." In eLSE 2017. Carol I National Defence University Publishing House, 2017. http://dx.doi.org/10.12753/2066-026x-17-246.
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The modern approaches in teaching and learning for basic engineering sciences include the usage of computer simulations, collaborative learning, problem-based learning and Internet access. The cognitive process is a complicated one, and the learning methodologies must be often focused on particular area of knowledge. Feedback messages can help in process of improvement of learning process by adjusting the accessibility and addressability via e-learning technologies and application. The review encompass the main results in e-learning technology in education using desktop simulation tools, WEB3D methods and Internet possibilities as premise to have an improvement in e-learning. courses. Use of computers and new technologies have become a crucial part of learning as well as teaching. E-learning today has been a key factor in various industries and teaching is one among them. The Virtual universities as possible online courses are mentioned also by few significant papers. The biomedical engineering (BME) is in most of things a frontier domain and represents the application of engineering principles and design concepts to biology and medicine. Biomedical Engineering (BME) represents the application of engineering principles and design concepts to biology and medicine. Important tasks as data mining, image processing, gene sequencing requires management and processing large amount of data along with important workload and sharing among many users. Cloud computing can be used in these cases and a review of recent work in these new emerging technologies is made, also. The analysis and opportunity of this choice is motivated by conclusions from literature along with personal assertions. A possibility to use for validation of TAM (Theory of Acceptance Model) is proposed to be used for each case in a custom manner.
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Yang, Andy C., Hui-Huang Hsu, and Ming-Da Lu. "Applying gene ontology to microarray gene expression data analysis." In 2010 International Conference on System Science and Engineering (ICSSE). IEEE, 2010. http://dx.doi.org/10.1109/icsse.2010.5551740.
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Khalsan, Mahmood, Mu Mu, Eman Salih Al-shamery, Suraj Ajit, Lee Machado, and Michael Opoku Agyeman. "Developing a Multidimensional Fuzzy Deep Learning for Cancer Classification using Gene Expression Data." In 9th International Conference on Computer Science, Engineering and Applications. Academy & Industry Research Collaboration Center, 2023. http://dx.doi.org/10.5121/csit.2023.132304.
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In the realm of cancer research, the identification of biomarker genes plays a pivotal role in accurate classification and diagnosis. This study delves into the intersection of machine learning and gene selection to enhance the precision of biomarker identification for cancer classification. Leveraging advanced computational techniques. In the quest for improved cancer classification, studies face challenges due to high-dimensional gene expression data and limited gene relevance. To address these challenges, we developed a novel multidimensional fuzzy deep learning (MFDL) to select subset of significant genes and using those genes to train the model for better accuracy. MFDL is exploring the integration of fuzzy concepts within filter and wrapper methods to select significant genes and applying a fuzzy classifier to improve cancer classification accuracy. Through rigorous experimentation and validation, six gene expression data used, the findings demonstrated the efficacy of our methodology on diverse cancer datasets. The results underscore the importance of integrative computational methods in deciphering the intricate genomic landscape of cancer and spotlight the potential for improved diagnostic accuracy. The developed model showcased outstanding performance across the six employed datasets, demonstrating an average accuracy of 98%, precision of 98.3%, recall of 97.6%, and an f1-score of 97.8%.
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Karthik, D., and K. Kalaiselvi. "MRMR-GWICA: A hybrid gene selection and ensemble clustering framework for breast cancer gene expression data." In RECENT TRENDS IN SCIENCE AND ENGINEERING. AIP Publishing, 2022. http://dx.doi.org/10.1063/5.0074273.
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Sin-Liang Lim and Sim-Hui Tee. "Computational gene identification for colon cancer using Digital Gene Expression Displayer." In 2011 IEEE Colloquium on Humanities, Science and Engineering (CHUSER). IEEE, 2011. http://dx.doi.org/10.1109/chuser.2011.6163871.
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Baust, John M., Robert G. Van Buskirk, and John G. Baust. "Cryopreservation of an Engineered Skin Equivalent: The Apoptosis Paradigm." In ASME 1999 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 1999. http://dx.doi.org/10.1115/imece1999-0586.
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Abstract The emergence of the tissue engineering sciences has led to an immediate need for cryopreservation protocols compatible with cell survival, cell-matrix binding and “scaffold” integrity. While numerous problems exist related to directional restrictions in cryoprotectant transport in tissue models, recent findings have demonstrated that the induction of specific molecular events, especially gene regulated cell death (apoptosis) is of significance. Accordingly, we report on a new strategy for the successful cryopreservation of a human skin equivalent. The integration of an intracellular-type cryopreservation solution containing dimethyl sulfoxide with and without apoptotic inhibitors provides a successful model for tissue preservation. With optimized formulations, post-thaw improvement of viability upwards of three-fold has been demonstrated when compared with traditional preservation methodologies. We conclude that 1) the use of an intracellular-type cryopreservation medium, Hypothermosol®, can improve post-thaw tissue construct integrity and cell viability, and 2) the inclusion of apoptotic inhibitors significantly improves cryopreservation outcome.