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Dissertations / Theses on the topic 'Gene expression in E. coli'

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Published: 4 June 2021

Last updated: 1 February 2022

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1

Liang, Wei-jun. "The glucuronide transport system of Escherichia coli." Thesis, University of Cambridge, 1993. https://www.repository.cam.ac.uk/handle/1810/251548.

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2

Carrington, Francis James. "Plasmid instability and gene expression in E. coli." Thesis, University of Surrey, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.293874.

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3

Samanta, Priyankar. "Gene Expression and Evolution in Escherichia Coli Biofilm." Diss., North Dakota State University, 2014. https://hdl.handle.net/10365/27425.

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Biofilms can be defined as a complex aggregation of bacterial communities that involves many gene regulatory mechanisms, as well as evolutionary processes to increase biodiversity. Specific Aim 1 used a gene regulation approach to identity novel targets for the development of biofilm prevention and treatment techniques. The goal was to determine genes that get expressed early in biofilm development (prevention targets) and genes that get expressed late and in the outer layer of the biofilm (treatment targets). Biofilm formation is regulated by numerous regulators, including the two-component o

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4

Jackson, Laura. "Oxygen-regulated gene expression in Escherichia coli and hypoxia-targeted gene therapy." Thesis, University of Sheffield, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.414676.

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5

Latimer, Joe. "Gene Expression and Elemental Variation in Escherichia Coli Communities." Thesis, University of Sheffield, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.500211.

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6

Meng, Wenmao. "FNR and oxygen-regulated gene expression in Escherichia coli." Thesis, University of Sheffield, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.266134.

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7

Vasina, Jess A. "Expression of recombinant proteins in Escherichia coli under the transcriptional control of the cold-shock inducible cspA promoter /." Thesis, Connect to this title online; UW restricted, 1997. http://hdl.handle.net/1773/9866.

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8

Moss, Michael T. "Cloning and expression of mycobacterial genes in Escherichia coli." Thesis, University of Surrey, 1987. http://epubs.surrey.ac.uk/847829/.

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The ability of Escherichia coli to use the expression signals of mycobacterial genes was tested by inserting fragments of M. bovis BCG DNA into the E. coli promoter-probe plasmid pKK232-8. Comparison with the promoter activity achieved following insertion of restriction fragments of the E. coli host into. pKK232-8 revealed that a significant proportion of M. bovis BCG promoters were functional in E. coli. These results confirmed the suitability of E. coli as a host for the cloning and expression of mycobacterial genes. Using a variety of E. coli cloning vectors (pBR322, pUC13, EMBL4 and gtll),

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9

Paradis, François William François William. "The expression of cellulomomas fimi cellulase genes in Brevibacterium lactofermentum and characterization of recombinant C. fimi B-glucosidase A from E coli." Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/30586.

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In the first part of this thesis, I describe the expression of C. fimi cellulase genes in the closely related Brevibacterium lactofermentum by generating a shuttle vector able to replicate selectively in the latter and carrying full length cellulase-encoding genes. The expression of those genes apparently originated from some unpredicted regulatory sequences, possibly located within the vector itself. The enzymatic activity was mostly found in the culture medium in B. lactofermentum indicating that the organism secreted the enzymes. The putative C. fimi promoter sequences did not function in B

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10

Shen, Weiping. "Regulation of Escherichia coli pyrBI Gene Expression in Pseudomonas fluorescens." Thesis, University of North Texas, 1995. https://digital.library.unt.edu/ark:/67531/metadc278188/.

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Pseudomonas fluorescens does not appear to regulate the enzymes of de novo pyrimidine biosynthesis at the level of gene expression. Little or no apparent repression of pyr gene expression is observed upon addition of exogenous pyrimidines to the growth medium. The Escherichia coli pyrBI genes for aspartate transcarbamoylase (ATCase) were sized down and cloned into the broad host range plasmid, pKT230. Upon introduction into a P.fluorescenspyrB mutant strain, ATCase showed repression in response to exogenously fed pyrimidine compounds. Thus, it was possible to bring about changes in pyrimidine

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11

Unoson, Cecilia. "Small RNA-mediated Regulation of Gene Expression in Escherichia coli." Doctoral thesis, Uppsala universitet, Institutionen för cell- och molekylärbiologi, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-130885.

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Non-coding RNAs are highly abundant regulators of gene expression in all kingdoms of life that often play important roles in vital cellular functions. In bacteria, small regulatory RNAs (sRNAs) usually act post-transcriptionally by regulating mRNAs through base pairing within ribosome binding sites (RBS), thereby inhibiting translation initiation. tisB encodes a toxin, TisB, whose synthesis is controlled by the sRNA IstR-1. Intriguingly, IstR-1 base pairs far upstream of the RBS but nevertheless inhibits translation initiation. The tisB mRNA is unusual in that ribosomes cannot access the RBS d

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12

Owolabi, Joshua Babatunde. "Characterization and expression of Cellulomonas fimi endoglucanase B gene and properties of the gene product from Escherichia coli." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/29044.

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In Cellulomonas fimi the cenB gene encodes a secreted endoglucanase (EngB) involved in the degradation of cellulose. The cenB gene carried on a 5.6 kb C fimi DNA fragment encodes a polypeptide of Mr 110,000 in Escherichia coli. The level of expression of the gene was significantly increased by replacing its normal transcriptional and translational regulatory signals with those of the E. coli lac operon. The intact EngB polypeptide is not required for enzymatic activity: active polypeptides of Mr 95,000 and 82,000 also appear in E. coli and a deletion mutant of cenB encodes an active polypeptid

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13

Ralphs, N. T. "An immunological and genetic dissection of the #beta# subunit of E. coli RNA polymerase." Thesis, University of Nottingham, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.235392.

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14

Gonzalez, de Valdivia Ernesto I. "Studies on translation initiation and gene expression in Escherichia coli." Doctoral thesis, Stockholm : Department of Genetics, Microbiology and Toxicology, Stockholm University, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-985.

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15

Walters, Robin George. "The organisation, expression and function of the UVRC gene of Escherichia coli." Thesis, University of Liverpool, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.237558.

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16

Col, Bekir. "Regulation of Fructose 1,6-bisphosphatase II (GlpX) Gene Expression in Escherichia coli." Diss., Virginia Tech, 2004. http://hdl.handle.net/10919/11282.

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The glpX gene of Escherichia coli encodes fructose 1,6-bisphosphatase II (FBPase II), an enzyme that would appear to be redundant with FBPase I, encoded by fbp. However, glpX mutants have no apparent phenotype, while fbp mutants are unable to grow on gluconeogenic substrates as sole carbon sources, suggesting that GlpX function is insufficient for growth of fbp mutants under these conditions. To gain insight into the physiological functions of the FBPases, regulation of glpX expression was investigated. It was found that glpX is transcribed as part of a complex glpFKX operon containing prom

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17

Poonlapthawee, Sirirat. "Gene expression and antibiotic resistance in Escherichia coli from Swedish inland waters." Licentiate thesis, Örebro universitet, Institutionen för naturvetenskap och teknik, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-28741.

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Extensive use of antibiotics both from human-medicine and veterinary sources are believed to provide selective pressure on bacteria that leads to an increase in antibiotic resistance in environmental waters. Contamination of antibiotic resistant microbes will raise human health risks. Escherichia coli are Gram negative bacilli that belong to the coliform group. E. coli are used as fecal indicators organism (FIO) to determine microbial contamination and water quality. We aimed to investigate the prevalence of antibiotic resistant bacteria in Swedish inland waters and determine the response of u

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18

Brierley, I. "Studies on DNA binding and the activation of transcription by the cyclic AMP receptor protein of Escherichia coli." Thesis, University of York, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.375422.

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19

Mehraein-Ghomi, Farideh. "Analysis of the assembly of a eukaryotic glucose transporter into the Escherichia coli cytoplasmic membrane." Thesis, University of Sussex, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.284076.

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20

Chen, Guozhu Schellhorn H. E. "Studies on the control of gene expression of rpoS and its regulon in Escherichia coli /." Also available via World Wide Web, 2003.

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21

Blomfield, Ian Charles. "Phase variation and expression of type 1 piliation in Escherichia coli : the role of integration host factor." Thesis, University of York, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.235659.

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22

Brown, Michael Edward. "Growth, metabolism and product expression in E. coli containing dual origin plasmids in batch and continuous culture." Thesis, University of Surrey, 1990. http://epubs.surrey.ac.uk/843792/.

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Efficient expression of recombinant protein was achieved in E. coli through the use of vectors capable of copy number amplification. Expression was regulated by the tryptophan promoter and plasmid copy number by the temperature sensitive Lambda Pg promoter. Reproducible expression of a variety of recombinant proteins was achieved in defined medium under fermentation conditions. In all cases, cell growth was strongly inhibited after amplification of both plasmid DNA and product expression. Plasmid copy number control was studied in continuous culture. Below 36°G, plasmid DNA was maintained

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23

Henderson, Ian. "Solving the inclusion body problem - a case study : high level expression of TEM-1 #beta#-lactamase in Escherichia coli." Thesis, University of Warwick, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.282432.

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24

McGlynn, Elaine. "The cloning and expression of the hepatitis B polymerase gene in Escherichia coli." Thesis, University of Edinburgh, 1987. http://hdl.handle.net/1842/15334.

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25

Mujacic, Mirna. "Characterization of regulation and expression patterns of Escherichia coli Hsp31 protein /." Thesis, Connect to this title online; UW restricted, 2006. http://hdl.handle.net/1773/8119.

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26

Brambilla, Elisa. "Investigation of E. coli genome complexity by means of fluorescent reporters of gene expression." Thesis, Paris 6, 2014. http://www.theses.fr/2014PA066607/document.

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Escherichia coli est capable de survivre dans de nombreux environnements différents. Les informations nécessaires à cette adaptation sont codées dans le chromosome. Cette molécule circulaire est condensé dans une structure compacte protéines-ADN, appelée nucléoïde. Le chromosome n¿est pas uniforme et montre notamment une distribution inégale de sites de fixation de protéines et de séquences riches en AT. Il a été montré que la position des gènes importants pour la cellule est hautement conservée dans les gamma-protéobactéries. Ces différences le long du chromosome et cette conservation de la p

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27

Pavitt, Graham David. "DNA supercoiling and regulation of gene expression in S. typhimurium and E. coli." Thesis, University of Oxford, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.315963.

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28

Taylor, Hannah Louise. "Quantitative detection of low abundance gene expression products in individual E. coli cells." Thesis, University of Edinburgh, 2018. http://hdl.handle.net/1842/31258.

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Stochastic fluctuations in mRNA and protein copy number between cells are inevitable during the process gene expression, even when cells carry identical chromosomes. Such fluctuations are able to impact the phenotypic fate of the cell, and are known to have greater impact when the copy number of the molecule involved is low. Additionally, up to 50% of proteins in Escherichia coli are present in the cell at a level of 10 molecules per cell or fewer (Taniguchi et al. 2010). As such, quantification of low copy number gene expression products and their distribution in cellular populations is key i

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29

Deneer, Henricus Gerardus. "Cloning and expression of Bacillus subtilis ribosomal RNA gene promoters in Escherichia coli." Thesis, University of British Columbia, 1986. http://hdl.handle.net/2429/27066.

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In coli, ribosomal RNA (rRNA) is synthesized in proportion to the cellular growth rate squared, a phenomenon known as growth rate dependent regulation. The promoters of rRNA operons, which consist of two tandemly arranged -35, -10 regions about 120 bp apart, mediate this characteristic form of regulation. To provide insight into how this regulation is achieved and to extend previous observations from studies with E. coli to the rRNA operons of other species, the promoter region from the B. subtilis rrnB operon was cloned onto a transcription fusion plasmid such that the synthesis of chloramphe

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30

Wedgwood, Stephen. "A characterisation of two genes of Escherichia coli, and the possible effect of BIMEs on gene expression." Thesis, University of Edinburgh, 1996. http://hdl.handle.net/1842/13220.

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In the course of work aimed at discovering genes encoding novel sigma subunits of <I>Escherichia coli </I>RNA polymerase, two unknown open reading frames were identified by hybridisation of an <I>E. coli </I>genome library with synthetic oligonucleotide probes directed against the conserved subregion 2.1 of bacterial sigma factors. This thesis describes the mapping, sequencing, and characterisation of these genes. The open reading frame <I>f229</I> was located near 4324kb on the physical map of the <I>E. coli</I> K12 chromosome. Its sequence and that of a downstream Bacterial Interspersed Mosa

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31

Cooper, Kerri W. "Expression, degradation, and applications of Escherichia coli TolA-beta-lactamase fusion proteins /." Thesis, Connect to this title online; UW restricted, 1998. http://hdl.handle.net/1773/9815.

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32

Paiva, Franciely Paula Toniolo de. "Quorum sensing em Escherichia coli enteropatogênica atípica." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-23032011-172812/.

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Escherichia coli enteropatogênica atípica (aEPEC) faz parte de um grupo de patógenos capazes de formar um tipo de lesão característica em cultura de tecidos epiteliais, denominada attaching and effacing (A/E). Os genes que são necessários para produção da lesão A/E estão localizados em uma ilha de patogenicidade denominada região LEE (locus of enterocyte effacement). A transcrição de genes da região LEE está sujeita a regulação por vários fatores, entre eles quorum sensing, termo utilizado para designar um mecanismo de regulação gênica dependente da concentração celular. Esse mecanismo é usado

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33

Ledala, Nagender Jayaswal Radheshyam K. "Molecular characterization of fur and transcriptional profiling of fur- and iron- regulated gene expression in Listeria monocytogenes." Normal, Ill. : Illinois State University, 2007. http://proquest.umi.com/pqdweb?index=0&did=1432808101&SrchMode=2&sid=8&Fmt=2&VInst=PROD&VType=PQD&RQT=309&VName=PQD&TS=1216230236&clientId=43838.

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Thesis (Ph. D.)--Illinois State University, 2007.<br>Title from title page screen, viewed on July 16, 2008. Dissertation Committee: Radheshyam K. Jayaswal (chair), Brian J. Wilkinson, Anthony J. Otsuka, Wade A. Nichols, Laura A. Vogel. Includes bibliographical references and abstract. Also available in print.

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34

Ownis, Ali A. "Verocytotoxin expression by E. coli O157:H7." Thesis, University of Warwick, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.343149.

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35

Haines, Sara. "Identification of environmental and genetic factors influencing virulence gene expression in Enterotoxigenic Escherichia coli." Thesis, Lyon 1, 2015. http://www.theses.fr/2015LYO10017.

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36

Charpentier, Bruno. "Étude des promoteurs transcriptionnels des gènes gapA et gapB codant pour la glycéraldéhyde-3-phosphate déshydrogénase d'Escherichia coli. : relation structure/fonction." Nancy 1, 1994. http://www.theses.fr/1994NAN10307.

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L'expression des gènes gapA et gapB d'Escherichia coli, codant pour glycéraldéhyde-3-phosphate déshydrogénase, a été étudiée au niveau: organisation des régions promoteur et régulations transcriptionnelles. Des résultats inattendus ont été obtenus: la partie codante de gapA est précédée d'une région promoteur complexe permettant une forte expression, quelles que soient les conditions environnementales, 4 promoteurs ont été mis en évidence. Les promoteurs p1, p3 et p4 sont reconnus par l'ARN polymérase esigma70, alors que le promoteur p2 est reconnu par l'holoenzyme esigma32. Le promoteur p1 es

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37

Kimmitt, Patrick Thomas. "Expression of Shiga toxin genes in Escherichia coli." Thesis, University of Newcastle Upon Tyne, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.299614.

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38

Lamprecht, Olga [Verfasser], and Victor [Akademischer Betreuer] Sourjik. "Heterogeneity of gene expression during biofilm formation in Escherichia coli / Olga Lamprecht ; Betreuer: Victor Sourjik." Marburg : Philipps-Universität Marburg, 2019. http://d-nb.info/1199537411/34.

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39

Abo-Amer, Aly El-Sayed Aly. "Molecular biological analysis of DcuS-DcuR controlling Câ‚„-dicarboxylate dependent gene expression in Escherichia coli." Thesis, University of Reading, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.397829.

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40

Shuttleworth, Helen L. "Studies on the expression of a cloned Staphylococcus aureus protein A gene in Escherichia coli." Thesis, University of Warwick, 1991. http://wrap.warwick.ac.uk/110354/.

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The gene coding for staphylococcal protein A has been cloned and the nucleotide sequence coding for the structural gene and its 5' flanking region determined. The DNA sequence showed the gene to be composed of a series of repetitive sequences, with five 120 nucleotide repeats comprising the 5' part of the structural gene and ten 24 nucleotide repeats in a continuous sequence at the 3’ terminus. Analysis of codon usage of the gene and comparison with other S. aureus genes revealed that spa showed a higher degree of homology with S. aureus plasmid genes than with chromosomally- located genes. Co

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41

Wright, Edwina M. "High level gene expression in E. coli : effects of point mutations within the coding sequence." Thesis, University of Leicester, 1988. http://hdl.handle.net/2381/34377.

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E.coli vectors having thermo-inducible copy number have been described by Yarranton et al. (1984). This thesis describes modification of such vectors to improve both their segregational stability and their host strain versatility. The modified vector system was initially used to demonstrate efficient, controllable expression of two inserted genes, one prokaryotic and one eukaryotic. This stable and efficient vector system was subsequently used to overexpress the eukaryotic gene TIMP (tissue inhibitor of metalloproteinases). Examination of expression levels of the recombinant protein revealed t

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42

Lee, Byeong-ha. "Gene expression under cold stress in Arabidopsis." Diss., The University of Arizona, 2004. http://hdl.handle.net/10150/290038.

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Microarray analysis and mutational approaches were used to investigate regulation of freezing tolerance in plants. Using Arabidopsis, cold-responsive gene expression profiling was performed with Affymetrix GeneChip. Arabidopsis seedlings were cold-treated at 0°C for 0, 3, 6, and 24 hrs. A total of 681 cold-responsive genes were identified. Comparison of the expression profile of cold-responsive genes in the wild type to that of ice1, a mutant defective in cold stress signaling, showed altered transcript levels for many cold-responsive genes in the ice1 mutant even under non-stress conditions

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43

Izard, Jerome. "Contrôle de la croissance et régulation génique chez Escherichia coli." Thesis, Grenoble, 2012. http://www.theses.fr/2012GRENV061/document.

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La faculté d’adaptation aux conditions environnementales des bactéries provient de lacomplexité de leur réseau de régulation génique, impliquant de nombreux régulateursspécifiques et la machinerie d’expression génique. Nous avons montré que le gène crl, codantun régulateur global d’Escherichia coli, est exprimé de façon transitoire lors de laphase exponentielle. Notre étude a permis d’identifier deux régulateurs responsables dece profil parmi une centaine testés. Ainsi, le complexe CRP-AMPc, réprime de façon indirectel’expression de crl, tandis que la nucléoprotéine Fis se fixe sur le promoteu

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44

Gockel, Jonas. "Deciphering regulatory mechanism influencing qepA efflux pump expression in Escherichia coli." Thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-410182.

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QepA is a plasmid-mediated efflux pump found in some strains of Escherichia coli, in which it significantly elevates the resistance against quinolones. The protein has similarities with 14-TMS major facilitator superfamily transporters and is situated in the inner membrane of the bacteria. It was acquired by horizontal gene transfer and integrated into a now inactivated class 1 integron, also harbouring several other antibiotic resistance genes such as rmtB and blaTEM-1. QepA alone is not sufficient to raise the resistance level over the clinical breakpoint and is in clinical isolates therefor

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45

Martins, Leonardo Pedro Donas-Boto de Vilhena. "Stochastic model of transcription initiation of closely spaced promoters in escherichia coli." Master's thesis, Faculdade de Ciências e Tecnologia, 2011. http://hdl.handle.net/10362/7009.

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Dissertação para obtenção do Grau de Mestre em Engenharia Biomédica<br>The regulatory mechanisms of transcription allow organisms to quickly adapt to changes in their environment and often act during transcription initiation. Here, a stochastic model of transcription initiation at the nucleotide level is proposed to study the dynamics of RNA production in closely spaced promoters and their regulatory mechanisms. We study how different arrangements (convergent e divergent), distance between transcription start sites (TSS), and various kinetic parameters affect the dynamics of RNA production.

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46

Chen, Huiyi. "System-Wide Studies of Gene Expression in Escherichia coli by Fluorescence Microscopy and High Throughput Sequencing." Thesis, Harvard University, 2011. http://dissertations.umi.com/gsas.harvard:10044.

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Gene expression is a fundamental process in the cell and is made up of two parts – the information flow from DNA to RNA, and from RNA to protein. Here, we examined specific sub-processes in Escherichia coli gene expression using newly available tools that permit genome-wide analysis. We begin our studies measuring mRNA and protein abundances in single cells by single-molecule fluorescence microscopy, and then focus our attention to studying RNA generation and degradation by high throughput sequencing. The details of the dynamics of gene expression can be observed from fluctuations in mRNA and

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47

楊奇凌. "Expression study of escherichia coli recO gene." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/24684901128974668548.

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48

Su, Yi-Cheng, and 蘇宜成. "Expression studies of escherichia coli recR gene." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/31943983054646914897.

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碩士<br>國立陽明大學<br>遺傳學研究所<br>82<br>recR基因是大腸桿菌細胞中與DNA重組有關的基因之一，其基因產物則為參與RecF重組途徑之作用成員之一。recR基因之發現雖然早在1989年，其選殖與定序亦於稍後陸續完成，但是關於Recf蛋白生化功能之研究與探討卻至今仍無明顯之進展。本篇論文所探討的主題是recR基因之表現，目的是希望能大量表現RecR蛋白以利其純化及將來生化功能之探討。另外，本文也分析RecR蛋白質大量表現的情況下對於細胞生理功能之影響。為了達成上述目的，在我們的研究中選用兩種具有不同啟動子的表現載體：分別是具有Ptac啟動子的pKK388-1以及具有T7ψ10啟動子的PET-3d。並利用分子選殖技術將recR基因序列之結構部份接於兩種表現載體之上。經由IPTG誘導後以SDS-PAGE分析細胞中RecR蛋白之表現，我們發現具有T7ψ10啟動子之表現載體可大量表現RecR，而其有Ptac啟動子的載體則僅微量表現RecR。另在誘導RecR蛋白表現的情況下，我們發現細胞之DNA修復作用及細胞存活性之變化與基礎表現狀態下比較並無明顯之差異。此結果顯示RecR之大量表現對於細胞之正常生理功能並無明顯之影響。為進一步了解RecR蛋白之生化功能

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49

Chang, Chun-Yang, and 張鈞暘. "Regulation of grxD gene expression in Escherichia coli." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/30229609007110434623.

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碩士<br>臺灣大學<br>農業化學研究所<br>96<br>This study was investigated the regulation of grxD gene expression in Escherichia coli. Based on different lengths of promoter fragment in grxD-lacZ operon and protein fusion, we have found that the gene expression was increased upon entry into stationary phase, and the expression of grxD was enhanced by two promoters P1grxD and P2grxD. While either promoter mutated at the location of -35 (P1grxD) and -10 (P2grxD) respectively, the promoter activity was decreased. In log phase, the grxD was more activated with P1grxD promoter than that with P2grxD. Under anaerobi

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Chuang, Shuang-En. "Global regulation of gene expression in Escherichia coli." 1992. http://catalog.hathitrust.org/api/volumes/oclc/28622161.html.

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