Relevant bibliographies by topics / Gene fragment

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Gene fragment'

Author: Grafiati
Published: 28 May 2022
Last updated: 30 July 2025

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Journal articles on the topic "Gene fragment"

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Lindley, Helen K., V. Jayne Deeble, Ursula Peschke, Mary O'Neill, Simon Baumberg, and Jonathan Cove. "Dependence on reporter gene of apparent activity in gene fusions of a Streptomyces griseus streptomycin biosynthesis promoter." Canadian Journal of Microbiology 41, no. 4-5 (1995): 407–17. http://dx.doi.org/10.1139/m95-054.

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The adjacent genes strR–strA–strB1 lie within the large cluster of genes of streptomycin biosynthesis and resistance in Streptomyces griseus. sirR encodes a pathway-specific activator StrR, suggested by previous work to be either an antiterminator or a conventional activator, binding to its DNA target via a helix-turn-helix motif. strB1 is transcribed in an StrR-dependent fashion from a promoter (PstrB1) that lies downstream from strA; between PstrB1 and sirB1 there is a 300-bp leader region containing numerous inverted repeats that could represent modulatable transcription termination sites.
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Sumie, Akihiro, Tetsu Yamashiro, Kazutoshi Nakashima, Masaru Nasu, Makoto Watanabe, and Akira Nishizono. "Comparison of Genomic Structures and Antigenic Reactivities of Orthologous 29-Kilodalton Outer Membrane Proteins ofHelicobacter pylori." Infection and Immunity 69, no. 11 (2001): 6846–52. http://dx.doi.org/10.1128/iai.69.11.6846-6852.2001.

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ABSTRACT We purified a 29-kDa Helicobacter pylori outer membrane protein (Omp29 protein) and cloned the gene encoding the protein from H. pylori strain ATCC 43504. The Omp29 gene corresponded to the reported JHP73 and the HP78–79 genes of H. pylori strains. A corresponding nucleotide fragment was detected in all 150 tested H. pylori clinical isolates by PCR or Southern blotting. The amplified Omp29-corresponding fragments were categorized into a ca. 770-bp-long group and a larger-fragment group. Sequence analysis indicated that the larger fragments were likely synthesized from the 770-bp fragm
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Barlow, J. W., L. E. Raggatt, C. C. Drinkwater, I. G. Lyons, and R. I. Richards. "Differential binding of thyroid hormone receptors to mouse glandular kallikrein gene promoters: evidence for multiple binding regions in the mGK-6 gene." Journal of Molecular Endocrinology 3, no. 2 (1989): 79–84. http://dx.doi.org/10.1677/jme.0.0030079.

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ABSTRACT We have used a DNA-cellulose competition assay to investigate the binding of thyroid hormone receptors to fragments of the mouse glandular kallikrein genes and the human and rat GH genes. Nuclear extracts from human lymphoblastoid IM-9 cells were incubated with [125I]tri-iodothyronine ([125I]T3) and DNA-cellulose. The ability of cloned gene fragments to compete for radiolabelled receptors bound to DNA-cellulose was compared with that of DNA from pBR322. As previously observed, a 900 bp fragment from the human GH gene showed preferential binding to the thyroid hormone receptor. High-af
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Inoue, Y., S. Kobayashi, K. Yoshikawa, LT Tran, and A. Kimura. "Lipid hydroperoxide‐resistance gene in Saccharomyces cerevisiae: utilization as a selectable marker gene for yeast transformation." Biotechnology and Applied Biochemistry 17, no. 3 (1993): 305–10. http://dx.doi.org/10.1111/j.1470-8744.1993.tb00246.x.

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Two kinds of DNA fragments concerned with resistance against lipid hydroperoxide were cloned from the yeast Saccharomyces cerevisiae. When the recombinant plasmid carrying the DNA fragment was introduced into the cells of S. cerevisiae which do not have an appropriate auxotrophic marker, the transformants could be selected by their resistance to t‐butyl hydroperoxide. By Southern hybridization analysis, such transformants were found to contain the recombinant plasmid, suggesting that the DNA fragment could be used as a selection marker for the transformation of S. cerevisiae.
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Messick, Joanne B., Geoffrey Smith, Linda Berent, and Sandra Cooper. "Genome size ofEperythrozoon suisand hybridization with 16S rRNA gene." Canadian Journal of Microbiology 46, no. 11 (2000): 1082–86. http://dx.doi.org/10.1139/w00-088.

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The genome size of Eperythrozoon suis, an unculturable haemotropic mycoplasma, was estimated using pulsed-field gel electrophoresis (PFGE). Gamma irradiation was used to introduce one (on the average) double-strand break in the E. suis Illinois chromosome. Restriction enzymes that cut infrequently were also used to analyze genome size. The size estimate for the full-length genome was 745 kilobases (kb), whereas the size estimates based on the summation of restriction fragments ranged from 730 to 770 kb. The 16S rRNA gene was located on the 120-kb MluI fragment, 128-kb NruI fragment, 25-kb SacI
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Djordjevic, G., B. Bojovic, N. Miladinov, and L. Topisirovic. "Cloning and molecular analysis of promoter-like sequences isolated from the chromosomal DNA ofLactobacillus acidophilusATCC 4356." Canadian Journal of Microbiology 43, no. 1 (1997): 61–69. http://dx.doi.org/10.1139/m97-009.

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Promoter-like sequences from the chromosomal DNA of thermophilic strain Lactobacillus acidophilus ATCC 4356 were cloned. Analysis of the three DNA fragments showing promoter activity, designated P3, P6, and P15, were performed in Lactobacillus reuteri, Lactococcus lactis, and E. coli. The reporter cat-86 gene was expressed in all three bacterial species under control of the fragments P3 and P6. Fragment P15 showed promoter activity only in Lactobacillus reuteri and E. coli but not in Lactococcus lactis. The three host-specific transcriptional start points (TSPs) were used when transcription of
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Li, Xiu-min, Xing-long Bai, and Guo-dong Ren. "A new species of the genus Blaptogonia from the Himalayas with four DNA markers (Coleoptera, Tenebrionidae, Blaptini)." ZooKeys 773 (July 9, 2018): 69–78. http://dx.doi.org/10.3897/zookeys.773.24656.

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A new species of the genus Blaptogonia Medvedev, 1998, B.zhentanga sp. n., is described from the southern Himalayas of China. Two fragments of mitochondrial protein-coding genes (COI, Cytb), one fragment of mitochondrial ribosomal RNA gene (16S), and one fragment of nuclear rRNA gene (28SD2) of the new species were obtained. A key to the known species of the genus is presented.
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Li, Xiu-Min, Xing-Long Bai, and Guo-Dong Ren. "A new species of the genus Blaptogonia from the Himalayas with four DNA markers (Coleoptera, Tenebrionidae, Blaptini)." ZooKeys 773 (July 9, 2018): 69–78. https://doi.org/10.3897/zookeys.773.24656.

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A new species of the genus Blaptogonia Medvedev, 1998, B. zhentanga sp. n., is described from the southern Himalayas of China. Two fragments of mitochondrial protein-coding genes (COI, Cytb), one fragment of mitochondrial ribosomal RNA gene (16S), and one fragment of nuclear rRNA gene (28SD2) of the new species were obtained. A key to the known species of the genus is presented.
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Shigematsu, Toru, Satoshi Hanada, Masahiro Eguchi, Yoichi Kamagata, Takahiro Kanagawa, and Ryuichiro Kurane. "Soluble Methane Monooxygenase Gene Clusters from Trichloroethylene-Degrading Methylomonas sp. Strains and Detection of Methanotrophs during In Situ Bioremediation." Applied and Environmental Microbiology 65, no. 12 (1999): 5198–206. http://dx.doi.org/10.1128/aem.65.12.5198-5206.1999.

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ABSTRACT The soluble MMO (sMMO) gene clusters from group I methanotrophs were characterized. An 8.1-kb KpnI fragment fromMethylomonas sp. strain KSWIII and a 7.5-kbSalI fragment from Methylomonas sp. strain KSPIII which contained the sMMO gene clusters were cloned and sequenced. The sequences of these two fragments were almost identical. The sMMO gene clusters in the fragment consisted of six open reading frames which were 52 to 79% similar to the corresponding genes of previously described sMMO gene clusters of the group II and group X methanotrophs. The phylogenetic analysis of the predicted
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Reid, L. H., E. G. Shesely, H. S. Kim, and O. Smithies. "Cotransformation and gene targeting in mouse embryonic stem cells." Molecular and Cellular Biology 11, no. 5 (1991): 2769–77. http://dx.doi.org/10.1128/mcb.11.5.2769-2777.1991.

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We have investigated cotransformation in mammalian cells and its potential for identifying cells that have been modified by gene targeting. Selectable genes on separate DNA fragments were simultaneously introduced into cells by coelectroporation. When the introduced fragments were scored for random integration, 75% of the transformed cells integrated both fragments within the genome of the same cell. When one of the cointroduced fragments was scored for integration at a specific locus by gene targeting, only 4% of the targeted cells cointegrated the second fragment. Apparently, cells that have
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Dissertations / Theses on the topic "Gene fragment"

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Roy, Poorna. "Deconstructing the ribosome: specific interactions of a ribosomal RNA fragment with intact and fragmented L23 ribosomal protein." Thesis, Georgia Institute of Technology, 2013. http://hdl.handle.net/1853/47579.

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The complexity of translation is a classical dilemma in the evolution of biological systems. Efficient translation requires coordination of complex, highly evolved RNAs and proteins; however, complex, highly evolved RNAs and proteins could not evolve without efficient translation system. At the heart of this complexity is the ribosome, itself a remarkably complex molecular machine. Our work illustrates the ribosome as deconstructed units of modification. Here we have deconstructed a segment of the ribosome to interacting RNA-protein units. L23 interacts in vivo with both Domain III (DIII)
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Andreu, Alice. "Tetanus toxin fragment C as a targeting moiety for gene delivery." Thesis, Imperial College London, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.419230.

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Taylor, Gillian. "Isolation of a genomic DNA fragment containing the oestrogen regulated gene LIV-1." Thesis, University of Liverpool, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.386854.

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Anamizu, Joy NP. "Characterization of Three Genes Located in a 4.0-KB Fragment Within the MID Gene Cluster of Rhizobium SP Strain TAL1145." Thesis, University of Hawaii at Manoa, 2002. http://hdl.handle.net/10125/6938.

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Rhizobium sp. strain TAL1145 is a soil bacterium that forms nitrogen-fixing root nodules on the tree-legume, Leucaena leucocephala. Leucaena produces a toxic non-protein amino acid, known as mimosine, which is also released in root exudates. Rhizobium sp. TAL1145 can degrade mimosine and use it as a source of carbon and nitrogen. The genes for mimosine degradation (mid genes) in TAL1145 have been recently isolated. The mid genes are located in two clusters within a 25-kb DNA fragment in the TAL1145 chromosome. The aim of this investigation is to identify and characterize the genes in a 4.0-kb
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Jaichuen, C., R. Chundet, L. D. Yu, P. Thongkumkoon, and S. Anuntalabhochai. "Effect on Genetic Mutation Induction from Nano-Ranged Low-Energy Plasma Ion Bombardment of DNA and Gene Fragment." Thesis, Sumy State University, 2012. http://essuir.sumdu.edu.ua/handle/123456789/34948.

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ISHIHARA, OSAMU, KENJI IKEBUCHI, CHIAKI SATO, ATSUO ITAKURA, MASAAKI HARA, and MACHIKO KIMURA. "FRAGMENT SIZE ANALYSIS OF FREE FETAL DNA IN MATERNAL PLASMA USING Y-STR LOCI AND SRY GENE AMPLIFICATION." Nagoya University School of Medicine, 2011. http://hdl.handle.net/2237/15355.

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LE, GUEN LAURENCE. "Etude d'un fragment genomique d'arabidopsis thaliana porteur d'un gene codant une proteine kinase homologue a snf1 de saccharomyces cerevisiae." Paris 11, 1994. http://www.theses.fr/1994PA112169.

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Les proteines kinases sont des elements cles des voies de transduction de signaux. Certaines d'entre elles, presentes chez tous les organismes, ont ete remarquablement conservees au cours de l'evolution. Dans un premier temps, un gene d'arabidopsis thaliana, akin10, a ete sequence. Le produit code par ce gene est apparente a la proteine serine/threonine kinase snf1 sucrose non fermenting) de saccharomyces cerevisiae, impliquee dans la voie de derepression des genes reprimes par le glucose, ainsi qu'a la proteine kinase activee par le 5-amp, intervenant dans l'inactivation de plusieurs enzymes
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Pavan, Tycha Bianca Sabaini 1983. "Detecção do gene 16Sr-RNA e identificação de patógenos bacterianos, causadores de sepse neonatal precoce, pela técnica da RFLP-PCR ("Restriction Fragment Length Polymorfism - Polymerase Chain Reaction")." [s.n.], 2013. http://repositorio.unicamp.br/jspui/handle/REPOSIP/309724.

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Orientador: Sergio Tadeu Martins Marba<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas<br>Made available in DSpace on 2018-08-23T19:06:43Z (GMT). No. of bitstreams: 1 Pavan_TychaBiancaSabaini_M.pdf: 1135745 bytes, checksum: 87193a679a977d0df4230db53eab4297 (MD5) Previous issue date: 2013<br>Resumo: O objetivo do trabalho foi descrever e avaliar a capacidade da "RFLP-PCR" para detectar patógenos bacterianos em recém-nascidos (RN) e determinar seu uso diagnóstico na sepse neonatal precoce (SNP). Foram avaliados RNs assintomáticos, filhos de gestante
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Masli, Aryananda. "Search for restriction fragment length polymorphism of Phaseolus vulgaris in relation to the immune gene to bean common mosaic virus." Thesis, University of North Texas, 1991. https://digital.library.unt.edu/ark:/67531/metadc798405/.

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A technique involving Restriction Fragment Length Polymorphism (RFLP) was used to observe the DNA fragment polymorphism between a bean cultivar with I/I genotype and a bean cultivar with i/i genotype. The I gene encodes immunity to bean common mosaic virus (BCMV).
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FILARETO, ANTONIO. "Application and optimization of a gene targeting strategy called Small Fragment Homologous Replacement for the treatment of the Spinal Muscolar Atrophy." Doctoral thesis, Università degli Studi di Roma "Tor Vergata", 2009. http://hdl.handle.net/2108/736.

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The majority of patients affected by spinal muscular atrophy (SMA) have deletion of the survival of motor neuron 1 (SMN1) gene, but they retain a “nonfunctional” copy of the duplicate gene (SMN2) in their genome. SMN2 produces defective SMN protein because of a C>T transition in exon 7, which causes the skipping of exon 7 during SMN mRNA maturation. Many attempts have been made to correct altered SMN gene expression and to increase the level of normal SMN protein, but to date an effective treatment for this disease has not been established. Small Fragment Homologous Replacement (SFHR) is a sit
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Books on the topic "Gene fragment"

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Zouganelis, George D. Cloning, sequencing and characterization of a chitin synthase gene fragment from the fungus Phascolomyces articulosus. Brock University, Dept. of Biological Sciences, 1997.

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Koivula, Teija. Characterization of genes and DNA-fragments affecting gene expression and protein secretion in Lactococcus lactis. T. Koivula, 1992.

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Xu, Fan. Construction of vaccinia virus recombinants carrying fragments of the rotavirus gene encoding VP7. typescript, 1994.

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Michael, Conn P., ed. Gene expression in neural tissues. Academic Press, 1992.

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Yun, Young-Sup. Cloning and mapping of mouse complement factor 1 gene fragments and identification of an additional exon not present in the human factor 1 gene. National Library of Canada, 1994.

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Roy, Josée Emilie. The Purification of DNA fragments from the homeobox gene, Hox-11, by mini-preparation and southern hybridization. Laurentian University, Department of Biology, 1993.

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Oelbaum, Raymond Stuart. An analysis of four candidate genes for non-insulin-dependent diabetes using restriction fragment length polymorphism markers. University of Manchester, 1994.

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Cortes, Marina Correa. Influence of gene dispersal and environmental heterogeneity on spatial and genetic patterns of the understory herb Heliconia acuminata across a fragmented landscape in central Amazon, Brazil. [publisher not identified], 2012.

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Michael, Conn P., ed. Lesions and transplantation. Academic Press, 1991.

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M, Conn P., ed. Methods in neurosciences. Academic Press, 1989.

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Book chapters on the topic "Gene fragment"

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Li, Yubin, and Hugo K. Dooner. "Helitron Proliferation and Gene-Fragment Capture." In Plant Transposable Elements. Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-31842-9_11.

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Winter, Christoph H., and Roland E. Kontermann. "Epitope Mapping with Gene Fragment Libraries." In Antibody Engineering. Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-662-04605-0_34.

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Kamitani, Hideki, Tomokatsu Hori, Satoshi Tanaka, et al. "Restriction Fragment Length Polymorphism of the L-myc Gene and Gene Amplification in Meningiomas." In Biological Aspects of Brain Tumors. Springer Japan, 1991. http://dx.doi.org/10.1007/978-4-431-68150-2_47.

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Calandra, S. "Studies on DNA fragment length polymorphism of human apolipoprotein B gene." In Atherosclerosis and Cardiovascular Diseases. Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3205-0_53.

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Reis, Gustavo, Nuno Fonseca, Francisco Fernández de Vega, and Anibal Ferreira. "Hybrid Genetic Algorithm Based on Gene Fragment Competition for Polyphonic Music Transcription." In Lecture Notes in Computer Science. Springer Berlin Heidelberg, 2008. http://dx.doi.org/10.1007/978-3-540-78761-7_31.

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Volk, Anna-Luisa, Francis Jingxin Hu, and Johan Rockberg. "Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Gene Fragment Libraries." In Epitope Mapping Protocols. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7841-0_10.

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Zhang, X., T. Nakaoka, Corey H. Mjaatvedt, Roger R. Markwald, and N. Yamashita. "Hdf-Affected Gene Fragment Revealed by Subtraction Study of HDF (Heart Defect) Mouse." In Cardiovascular Development and Congenital Malformations. Blackwell Publishing Ltd, 2007. http://dx.doi.org/10.1002/9780470988664.ch40.

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Volk, Anna-Luisa, Francis Jingxin Hu, Magdalena Malm, and Johan Rockberg. "Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Gene Fragment Libraries." In Methods in Molecular Biology. Springer US, 2025. https://doi.org/10.1007/978-1-0716-4591-8_9.

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Arimoto, Asuka, and Kuni Tagawa. "Studying Hemichordata WBR Using Ptychodera flava." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2172-1_15.

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AbstractHemichordates are benthic marine invertebrates closely related to chordates. Several species, including Ptychodera flava in the phylum Hemichordates, can undergo whole body regeneration from a small fragment. P. flava is widely distributed in the warm Indo-Pacific region and is easily collected in the lower tidal zone of a shallow beach with a coral reef. Here, we describe the methods for animal collection and preparation of regenerating tissues. The prepared tissues can be used for various molecular and/or histological experiments. We also demonstrate how to examine gene expression pa
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Schurr, E., E. Skamene, M. Nesbitt, R. Hynes, and P. Gros. "Identification of a Linkage Group Including the Bcg Gene by Restriction Fragment Length Polymorphism Analysis." In Genetics of Immunological Diseases. Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-50059-6_46.

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Conference papers on the topic "Gene fragment"

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Ploos van Amstel, J. K., A. L. van der Zanden, P. H. Reitsma, and R. M. Bertina. "RESTRICTION ANALYSIS AND SOUTHERN BLOTTING OF TOTAL HUMAN DNA REVEALS THE EXISTENCE OF MORE THAN ONE GENE HOMOLOGOUS WITH PROTEIN S cDNA." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644639.

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A deficiency in protein S, the cofactor of activated protein C, is associated with an increased risk for the development of venous thrombosis. It is inherited as an autosomal dominant disorder. To improve the detection of heterozygotes in affected families, we have started to search for restriction fragment length polymorphism (RFLP) in the protein S gene. This study revealed the existence of two genes containing sequences homologous to protein S cDNA.Three non-overlapping fragments of clone pSUL5, which codes for the carboxy-terminal part of protein S and contains the complete 3' untranslated
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Volinia, S., P. Patracchini, F. Vannini, L. Felloni, F. Panicucci, and F. Beranardi. "HAGEMAN TRAIT INVESTIGATED BY FACTOR XII cDNA PROBES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643299.

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The presence of gene lesions and of restriction fragment length polymorphisms (RFLPs) has been investigated by means of cDNA probes for the coagulation factor XII (FXII).A TaqI additional fragment (2.1Kb) has been found in two brothers with Hageman trait and in 11 members of their paternal lineage. Digestions with different enzymes exclude that FXII gene deletion is responsible for Hageman trait in this family. A point mutation originating an additional TaqI site is likely.The abnormal pattern (not present in 40 normal subjects) is correlated with a reduced FXII activity and identifies the het
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Ezerskii, V. A., E. M. Koloskova, and T. P. Trubitsina. "Green fluorescent protein gene for site-specific integration into the locus of the rabbit whey acidic protein gene." In CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-2020-5-9-10-129.

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The high content of whey acidic protein in rabbit milk makes the gene of this protein a promising candidate for its replacement by the gene of pharmacologically active protein using the CRISPR/Cas9 system. The plasmid that contains 5’ and 3’ arms of homology to the rabbit WAP gene was created. A fragment containing a green fluorescent protein gene under the CMV promoter has been integrated into this site. A strategy of making double-stranded cuts in the gene WAP and receiving four pX330 plasmids encoding the endonuclease Cas9 and guide RNAs was developed. The plasmid containing a fragment cmvE
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Matthews, R. J., I. R. Peake, and A. L. Bloom. "POINT-MUTATION OF FACTOR VIII CODING SEQUENCES IN HAEMOPHILIA A." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644013.

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In order to study the molecular basis of haemophilia A, DNA from 26 haemophilia A patients (8 severe with inhibitors, 13 severe noninhibitors and 5 mild/moderate) was screened by the Southern blotting method with FVIII cDNA probe A (a i.7kb Kpnl cDNA fragment that spans exons 1 to 12) probe B (a 4.7kb EcoRI cDNA fragment that contains exons 14 to 25 and part of exon 26) probe C (a 1.8kb EcoRI cDNA fragment that contains the remainder of exon 26 and probe D (an Apal/EcoRI 783bp cDNA fragment that includes all of exons 22 to 25 and parts of exons 21 and 26). All cDNA probes were kindly provided
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Chelucci, C., H. J. Hassan, R. Guerriero, A. Leonardi, G. Mattia, and C. Peschle. "POLYMORPHIC SITES IN FACTOR X GENE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643836.

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The structure of factor X gene has been analyzed by Southern blot in 5 subjects with factor X deficiency.Genomic DNA was digested with 8 different endonucleases and hybridized with a cDNA probe. The congenital deficiency observed in these patients is not apparently due to a major deletion or rearrangement. Since the gene locus is grossly intact, the disease presumably results from point mutation(s) not identified by the utilized endonucleases.Our study was also focused on the presence of polymorphic site(s) in the factor X gene locus. Analysis of 50 normal subjects allowed to identify several
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Matthews, R. J., D. S. Anson, I. R. Peake, and A. L. Bloom. "GENE DELETIONS IN THE FACTOR IX LOCUS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643871.

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Previous studies have indicated that the majority of haemophilia B patients who produce anti-factor IX inhibitors (antibodies) have some kind of deletion of the factor IX gene. We have analysed DNA from nine haemophilia B inhibitor patients using the Southern blotting method and hybridisation with (i) factor IX cDNA and intragenomic probes (ii) probes originating from flanking sequences up to 60kb 5' and 170kb 3' to the factor IX gene that have been isolated by gene walking experiments (D.S.Anson and G.G.Brownlee, unpublished observations).Two patients who are brothers (haemophilia B (Chicago
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John, John A. St, Erik Gafni, Brandon White, et al. "Abstract 4349: Predicting gene expression from plasma cell-free DNA using both the fragment length and fragment position." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.sabcs18-4349.

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John, John A. St, Erik Gafni, Brandon White, et al. "Abstract 4349: Predicting gene expression from plasma cell-free DNA using both the fragment length and fragment position." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.am2019-4349.

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Handa, M., K. Titani, K. Takio, and Z. M. Ruggeri. "CHARACTERIZATION OF THE VON WILLEBRAND FACTOR-BINDING DOMAIN OF PLATELET MEMBRANE GLYCOPROTEIN Ib." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642925.

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We have previously obtained immunochemical evidence that the von Willebrand factor (vWF)-binding domain of the platelet membrane glycoprotein (GP) Ib is located near the amino terminus of the a subunit (Journal of Biological Chemistry 261: 12579-12585, 1986). We have now determined the complete amino acid sequence of the 45 kDa tryptic fragment of glycocalicin that contains this domain. Purified glycocalicin was subjected to limited digestion with trypsin and the proteolytic fragments were separated by size-exclusion high-pressure liquid chromatography. Two fragments of 45 kDa and 84 kDa, resp
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Huber, P., J. Dalmon, M. Laurent, G. Courtois, D. Thevenon та G. Marguerie. "CHARACTERIZATION OFTHE 5’FLANKING REGION FOR THE HUMAN FIBRINOGEN β GENE". У XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642889.

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Fibrinogen is coded by three separate genes located in a 50kb region of chromosome 4 and organized in a α - β - γ orientation with an inversion of the gene 3- A human genomic library was constructed using the EMBL4 phage and screened with cDNA probes coding for human fibrinogen Aα, Bβ and γ chains. Clones, covering the fibrinogen locus,were identified, and their organization was analyzed by means of hybridization and restriction mapping. Among these clones one recombinant phage containing the β gene and large 5’ and 3’ -flanking sequences was isolated.To identify the regulatory sequences Dpstr
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Reports on the topic "Gene fragment"

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Porat, Ron, Doron Holland, and Linda Walling. Identification of Citrus Fruit-Specific and Pathogen-Induced Promoters and Their Use in Molecular Engineering. United States Department of Agriculture, 2001. http://dx.doi.org/10.32747/2001.7585202.bard.

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This one year BARD project was funded to develop methods to monitor promoter activity a gene expression patterns in citrus fruit. To fulfill this goal, we divided the research tasks between both labs so that the Israeli side evaluated the use of microprojectile bombardment ; a tool to evaluate transient gene expression in various citrus fruit tissues, and the US side optimized technical parameters required for Agrobacterium-mediated transformation of various citrus cultivars. Microprojectile bombardment appeared to be a very efficient method for transient gene expression analysis in citrus lea
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Barefoot, Susan F., Bonita A. Glatz, Nathan Gollop, and Thomas A. Hughes. Bacteriocin Markers for Propionibacteria Gene Transfer Systems. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7573993.bard.

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The antibotulinal baceriocins, propionicin PLG-1 and jenseniin G., were the first to be identified, purified and characterized for the dairy propionibaceria and are produced by Propionibacterium thoenii P127 and P. thoenii/jensenii P126, respectively. Objectives of this project were to (a) produce polyclonal antibodies for detection, comparison and monitoring of propionicin PLG-1; (b) identify, clone and characterize the propionicin PLG-1 (plg-1) and jenseniin G (jnG) genes; and (3) develop gene transfer systems for dairy propionibacteria using them as models. Polyclonal antibodies for detecti
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Tumwasorn, Somying, Ajcharaporn Sawatpanich, Nibondh Udomsantisuk, and Kamon Kawkitinarong. Development of test kit for detection of rifampin resistance in mycobacterium tuberculosis by PCR-reverse line blot hybridization. Faculty of Medicine, Chulalongkorn University, 2006. https://doi.org/10.58837/chula.res.2006.25.

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A genetic test by PCR-reverse line blot hybridization was developed for rapid detection of mycobacterium tuberculosis and rifampin resistance simultaneously. Duplex PCR targeting IS 6110 and rpoB gene was employed to detect M. tuberculosis and the rpoB fragment with rifampin resistance hot spot region. The analytical sensitivity of duplex PCR for IS 6110 and rpoB gene was found to be 10 and 100fg of M.Tuberculosis H37Rv DNA, respectively. Since duplex PCR was specific to M. tuberculosis complex, it was thus able to be applied directly in clinical specimens. Oligonucleotide probes were designed
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Sadka, Avi, Mikeal L. Roose, and Yair Erner. Molecular Genetic Analysis of Citric Acid Accumulation in Citrus Fruit. United States Department of Agriculture, 2001. http://dx.doi.org/10.32747/2001.7573071.bard.

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The acid content of the juice sac cells is a major determinant of maturity and fruit quality in citrus. Many citrus varieties accumulate acid in concentrations that exceed market desires, reducing grower income and consumer satisfaction. Pulp acidity is thought to be dependent on two mechanisms: the accumulation of citric acid in the vacuoles of the juice sac cells, and acidification of the vacuole. The major aim of the project was to direct effort toward understanding the mechanism of citric acid accumulation in the fruit. The following objectives were suggested: Measure the activity of enzym
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Levisohn, Sharon, Maricarmen Garcia, David Yogev, and Stanley Kleven. Targeted Molecular Typing of Pathogenic Avian Mycoplasmas. United States Department of Agriculture, 2006. http://dx.doi.org/10.32747/2006.7695853.bard.

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Intraspecies identification (DNA "fingerprinting") of pathogenic avian mycoplasmas is a powerful tool for epidemiological studies and monitoring strain identity. However the only widely method available for Mycoplasma gallisepticum (MG) and M. synoviae (MS)wasrandom amplified polymorphic DNA (RAPD). This project aimed to develop alternative and supplementary typing methods that will overcome the major constraints of RAPD, such as the need for isolation of the organism in pure culture and the lack of reproducibility intrinsic in the method. Our strategy focussed on recognition of molecular mark
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Lers, Amnon, and Gan Susheng. Study of the regulatory mechanism involved in dark-induced Postharvest leaf senescence. United States Department of Agriculture, 2009. http://dx.doi.org/10.32747/2009.7591734.bard.

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Postharvest leaf senescence contributes to quality losses in flowers and leafy vegetables. The general goal of this research project was to investigate the regulatory mechanisms involved in dark-induced leaf senescence. The regulatory system involved in senescence induction and control is highly complex and possibly involves a network of senescence promoting pathways responsible for activation of the senescence-associated genes. Pathways involving different internal signals and environmental factors may have distinctive importance in different leaf senescence systems. Darkness is known to have
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Hedrick, Ronald, and Herve Bercovier. Characterization and Control of KHV, A New Herpes Viral Pathogen of Koi and Common Carp. United States Department of Agriculture, 2004. http://dx.doi.org/10.32747/2004.7695871.bard.

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In this project we proposed to characterize the virus genome and the structural virion polypeptides to allow development of improved diagnostic approaches and potential vaccination strategies. These goals have been mostly achieved and the corresponding data were published in three papers (see below) and three more manuscripts are in preparation. The virion polypeptides of KHV strains isolated from USA (KHV-U) and Israel (KHV-I) were found to be identical. Purified viral DNA analyzed with a total of 5 restriction enzymes demonstrated no fragment length polymorphism between KHV-I and KHV-U but b
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Levisohn, Sharon, Mark Jackwood, and Stanley Kleven. New Approaches for Detection of Mycoplasma iowae Infection in Turkeys. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7612834.bard.

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Mycoplasma iowae (Mi) is a pathogenic avian mycoplasma which causes mortality in turkey embryos and as such has clinical and economic significance for the turkey breeder industry. Control of Mi infection is severely hampered by lack of adequate diagnostic tests, together with resistance to most antibiotics and resilience to environment. A markedly high degree of intra-species antigenic variation also contributes to difficulties in detection and control of infection. In this project we have designed an innovative gene-based diagnostic test based on specific amplification of the 16S rRNA gene of
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Wongpiyabovorn, Jongkonnee, Nattiya Hirankarn, Yingyos Avihingsanon, Tewin Tencomnao, Yong Poovorawan, and Kriangsak Ruchusatsawat. The association between immunogenetics and genetic susceptibility of psoriasis in Thai population. Chulalongkorn University, 2006. https://doi.org/10.58837/chula.res.2006.27.

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Psoriasis is T-cell-mediated skin autoimmunity, required environmental triggers and genetic susceptibility factors to become manifested. Psoriasis is a chronic skin disease characterized by the abnormal hyperproliferation and differentiation of the epidermis, elongated and prominent blood vessels and a thick perivascular lymphocytic infiltrate. Vascular endothelial growth factor (VEGF) gene play important role in pathogenesis of various diseases with angiogenic basis such as breast cancer and autoimmune disease including psoriasis. Many studies analyzed the association of VEGF gene polymorphis
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Ohad, Itzhak, and Himadri Pakrasi. Role of Cytochrome B559 in Photoinhibition. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7613031.bard.

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The aim of this research project was to obtain information on the role of the cytochrome b559 in the function of Photosystem-II (PSII) with special emphasis on the light induced photo inactivation of PSII and turnover of the photochemical reaction center II protein subunit RCII-D1. The major goals of this project were: 1) Isolation and sequencing of the Chlamydomonas chloroplast psbE and psbF genes encoding the cytochrome b559 a and b subunits respectively; 2) Generation of site directed mutants and testing the effect of such mutation on the function of PSII under various light conditions; 3)
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