Relevant bibliographies by topics / Gene primers

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Gene primers'

Author: Grafiati
Published: 7 June 2025
Last updated: 2 August 2025

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Journal articles on the topic "Gene primers"

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Larekeng, Siti Halimah, Firzan Nainu, Muh Restu, Ahmad Rifqi Makkasau, Nasri, and Yuni Fitri Cahyaningsih. "Development and testing of PHYA gene-specific primers in sorghum to detect light-tolerant traits." IOP Conference Series: Earth and Environmental Science 1471, no. 1 (2025): 012020. https://doi.org/10.1088/1755-1315/1471/1/012020.

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Abstract The PHYA gene is one of the genes that play a role in the shade avoidance mechanism. The study aimed to design specific primers for the PHYA gene in sorghum plants and optimize the DNA amplification process. Primers were designed using the Primer3 Plus online tool, and primers were generated and tested on 14 Indonesian sorghum varieties. Gene amplification with PCR technology requires PHYA-specific primers. The amplification rate reached 100% with aneling temperatures ranging from 54-55°C specific to each primer. The resulting band was bright, firm, and specific, and the amplicon size
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Aurora, Dhea Apriano, Wilson Novarino, Djong Hon Tjong, et al. "Primer Design of Sumatran Striped Rabbit (Nesolagus netscheri Schlegel, 1880) using Primer-BLAST and AliView Program." Jurnal Biologi Tropis 25, no. 1 (2025): 599–605. https://doi.org/10.29303/jbt.v25i1.8499.

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The Sumatran striped rabbit (Nesolagus netscheri) lacks specific primers to amplify the chytochrome oxidase 1 (CO1) gene and the chytochrome b (cytb) gene, at present. Therefore, it is important to design primers to amplify the CO1 gene and cytb gene in N. netscheri. The aim of this study is to compare the primer design methods used, namely Primer-BLAST and AliView programs, to design specific primers for the chytochrome oxidase 1 (CO1) and chytochrome b (cytb) genes in N. netscheri. This research was conducted using the descriptive method with molecular observation. In this study, CO1 gene pr
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Khaira, Annisa, Afifatul Achyar, Zulyusri Zulyusri, Yusni Atifah, Dwi Hilda Putri, and Violita Violita. "Primer Design and Optimization of Annealing Temperature for Analysis of Glutathione Reductase Gene Expression in Rice (Oryza sativa L.)." 3BIO: Journal of Biological Science, Technology and Management 5, no. 1 (2023): 142–48. http://dx.doi.org/10.5614/3bio.2023.5.1.3.

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Glutathione Reductase (GR) belongs to the NADPH-dependent flavoprotein oxidoreductase family and is found in both prokaryotes and eukaryotes. The GR gene is considered to play a key role in the elimination of oxidative reaction products by looking at the level of gene expression of GR rice in dealing with drought stress using qPCR. One of the important steps to develop a specific, effective and efficient qPCR is the primer design. Several studies analyzing GR gene expression in rice have also designed primers. However, the primer still lacks an ideal characteristic of primer, as it still has a
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Sozinova, O. I., N. A. Kozub, I. A. Sozinov, and Ya B. Blume. "Genome specificity of primers to puroindoline genes." Faktori eksperimental'noi evolucii organizmiv 22 (September 9, 2018): 191–96. http://dx.doi.org/10.7124/feeo.v22.947.

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Aim. Genome specificity of primers for amplification of complete coding sequences of puroindoline genes was studied. Methods. PCR with gene-specific primers was performed for amplification of puroindoline genes of wheat and related species. Results. The primer pair designated PinaDH is not gene-specific as it yields two products of amplification of the genes Pina-1 and Gsp-1. This primers pair is not specific for the Pina-1 gene of the genome V of D. villosum. The primer pairs designated PinaCM and Pinb are gene-specific as they produce one amplification product of respective length. We have d
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Semenov, V. M., S. K. Yahorau, I. A. Lyatos, et al. "REAL-TIME PCR TEST SYSTEM FOR TTV DNA DETECTION IN BIOLOGICAL MATERIAL." Hepatology and Gastroenterology 8, no. 1 (2024): 36–41. http://dx.doi.org/10.25298/2616-5546-2024-8-1-36-41.

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Background. The study of biological material for the presence of TTV DNA using the PCR method allows for a timely assessment of the functional state of the human liver and immune system. Objective. To develop components for real-time PCR for TTV DNA detection in biological material. Material and methods. The design and selection of optimal primers and probes (taking into account the size (length) of the amplicon, annealing temperature, nucleotide composition, distribution of nucleotides along the length of the primer, length of primers, the possibility of formation of hairpins and dimers by pr
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Mitin, Valentin, and Irina Mitina. "SOME ASPECTS OF PRIMER DESIGN FOR REAL TIME PCR WITH SYBR GREEN AS A DYE." Journal of Engineering Science XXVII (4) (December 15, 2020): 191–96. https://doi.org/10.5281/zenodo.4296193.

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This work considers the sequence of steps for designing primers for real time qPCR analysis with SYBR® Green as fluorescent dye. For primer design, we used Primer-Blast software. As an example, we design primers to fum6 gene, a member of fumonisin biosynthetic gene cluster. The importance of a balance between specificity and efficiency of primers obtained with the help of Primer-Blast software is discussed. Challenges of obtaining specific primers are considered, possible solutions are suggested. BLAST analysis of the primers is discussed, potential approaches to choosing an optimal primer
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Hidayah, Nurul, Yuni Ahda, Afifatul Achyar, and Yusni Atifah. "DESAIN PRIMER GEN EDNRB EKSON 4 DAN OPTIMASI PCR UNTUK ANALISIS MUTASI GEN PADA HIRSCHSPRUNG DISEASE." Jurnal Biogenerasi 10, no. 2 (2025): 1468–76. https://doi.org/10.30605/biogenerasi.v10i2.5954.

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EDNRB gene is a gene located on chromosome 13q22, plays a role in the signaling pathway during the development of the ENS intestinal nerves. Mutations in the EDNRB gene in humans are associated with Hirschsprung Disease (HD). Mutations in the EDNRB gene are the second most common cause after the RET gene in cases of HD with a percentage of 5–10%. To detect mutations, PCR products are needed. Primers are one of the basic components of PCR, while optimization of annealing temperature and optimization of primer concentration are factors in the success of primer attachment to the target gene. This
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Barak, Noga, Eduard Fadeev, Vera Brekhman, Dikla Aharonovich, Tamar Lotan, and Daniel Sher. "Selecting 16S rRNA Primers for Microbiome Analysis in a Host–Microbe System: The Case of the Jellyfish Rhopilema nomadica." Microorganisms 11, no. 4 (2023): 955. http://dx.doi.org/10.3390/microorganisms11040955.

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Amplicon sequencing of the 16S rRNA gene is extensively used to characterize bacterial communities, including those living in association with eukaryotic hosts. Deciding which region of the 16S rRNA gene to analyze and selecting the appropriate PCR primers remains a major decision when initiating any new microbiome study. Based on a detailed literature survey of studies focusing on cnidarian microbiomes, we compared three commonly used primers targeting different hypervariable regions of the 16S rRNA gene, V1V2, V3V4, and V4V5, using the jellyfish Rhopilema nomadica as a model. Although all pr
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Nitovska, І. О. "PECULIARITIES OF GREEN FLUORESCENT PROTEIN TRANSGENE DETECTION IN TOBACCO AND MAIZE PLANTS BY PCR." Biotechnologia Acta 16, no. 4 (2023): 44–49. http://dx.doi.org/10.15407/biotech16.04.044.

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The aim of the work was to investigate detection of different modifications of the green fluorescent protein gene (gfp) in the transgenic tobacco and maize plants by polymerase chain reaction (PCR). Methods. Total DNA isolation, PCR, electrophoresis of DNA in agarose gel, bioinformatic resources. Results. Three pairs of primers were used for PCR analysis of tobacco and maize containing wild-type gfp or mutant synthetic gene S65Tpgfp. The primer pair gfp1F-gfp1R interacted with the wild-type gfp gene only. The gfp2F-gfp2R primers interacted with the gfp gene of different modifications both in t
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Okazaki, Ryuji, Akira Ootsuyama, Yasuhiro Yoshida, and Toshiyuki Norimura. "Establishment of Methylation-Specific PCR for the Mouse p53 Gene." Molecular Biology International 2011 (December 12, 2011): 1–4. http://dx.doi.org/10.4061/2011/938435.

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Methylation-specific PCR (MSP) of the mouse p53 gene has not yet been reported. We searched the CpG islands, sequenced the bisulfited DNA, and designed PCR primers for methylation and unmethylation sites. DNA from a young mouse produced a strong PCR product with the unmethylated primer and a weaker band with the methylated primer. DNA from an old mouse produced bands of similar intensities with both primers. In radiation-induced tumors, DNA from an old mouse yielded similar bands with both types of primers. We suggest that MSP is a valuable technique for the epigenetic study of the mouse p53 g
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Dissertations / Theses on the topic "Gene primers"

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Ahmad, M. Mursaleen Cheng Jianlin. "MUPrimer a tool for finding allele specific PCR-primers for homologous gene sequences /." Diss., Columbia, Mo. : University of Missouri--Columbia, 2009. http://hdl.handle.net/10355/6660.

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Title from PDF of title page (University of Missouri--Columbia, viewed on March 10, 2010). The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file. Thesis advisor: Dr. Jianlin Cheng. Includes bibliographical references.
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Alonso, Gabriela Caroline. "Avaliação, desenho e padronização de primers para genes de virulência de Candida albicans e sua expressão em isolados clínicos submetidos à terapias antifúngicas /." Araraquara, 2017. http://hdl.handle.net/11449/152919.

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Orientador: Ana Cláudia Pavarina<br>Abstract: Este estudo avaliou longitudinalmente a expressão de genes de virulência de isolados clínicos de Candida albicans de pacientes com candidose oral tratados com Terapia Fotodinâmica Antimicrobiana (aPDT) ou Nistatina (NIS). Inicialmente, a especificidade de primers da literatura e novos primers desenhados para os genes de virulência de C. albicans ALS1, CAP1, CAT1, EFG1, HWP1, LIP3, PLB1, SAP1, SAP4, SOD1, SOD5 e ACT1 (gene de controle) foi avaliada através de análises in silico e in vitro. Para a análise in silico, foi realizada uma busca no Pubmed
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Babben, Steve [Verfasser]. "An efficient approach for the development of locus specific primers in wheat and its application in a candidate gene based association study on frost tolerance in wheat (Triticum aestivum L.) / Steve Babben." Halle, 2019. http://d-nb.info/1177798565/34.

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Münzner, Ulrike. "Gene fishing in Cataglyphis fortis – Identification of genes inthe desert ant." Thesis, Linköping University, Department of Physics, Chemistry and Biology, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-20455.

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<p>The desert ant Cataglyphis fortis lives in the Sahara desert where it is exposed to extreme temperatures up to 70° C. In other words, the organism is considered as a thermophile. Until now the genome remains unknown but the fact that C. fortis provides heat stable proteins makes it very interesting in the field of protein studies and maybe even therapeutical research later on. This thesis focuses on trying to find genes that are expressed in C. fortis. Different genes were chosen and capable primers designed. After fishing for the enzyme GAPDH a fragment was found and sequenced. The sequenc
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Souza, AlethÃia CarÃzia Baracho de Lima. "Estudos sobre as interaÃÃes das proteÃnas seminais com as cÃlulas espermÃticas e componentes dos diluidores usados na criopreservaÃÃo do sÃmen e sobre marcadores moleculares de parÃmetros do sÃmen em animais de produÃÃo." Universidade Federal do CearÃ, 2014. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=12911.

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FundaÃÃo de Amparo à Pesquisa do Estado do CearÃ<br>A tese à composta por dois capÃtulos. O primeiro capÃtulo inclui o trabalho cujo objetivo foi investigar o potencial uso de pares de transcritos correlativos baseados em microarranjos como marcadores de fertilidade masculina usando a displasia da bainha fibrosa (DFS) como modelo afetado. Atualmente à bastante reconhecido que a tecnologia de microarranjos pode ser limitada pelos custos e que a qualidade dos transcritos permanece relativamente desconhecida. Para responder essas questÃes, nÃs analizamos pares de transcritos estÃveis por qPCR com
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Mascarenhas, Soraia Andreia Coelho. "Nova abordagem para deteção das subespécies de Photobacterium damselae." Master's thesis, Universidade de Aveiro, 2013. http://hdl.handle.net/10773/12793.

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Mestrado em Biotecnologia - Biotecnologia Industrial e Ambiental<br>Em todo o mundo, os recursos para pesca encontram-se próximos dos limites de exploração sustentável. A prática de aquacultura é uma atividade que veio ajudar a contornar o esgotamento dos recursos pesqueiros, sendo hoje em dia responsável por contribuir com cerca de metade do fornecimento de peixe para consumo humano mundial. No entanto, a aquacultura é propícia ao aparecimento de surtos de doenças causadas por agentes patogénicos bacterianos. Estes geralmente têm um impacto nefasto nos sistemas de cultivo causando muitas veze
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Morley, Gavin W. "Gene hunting in primary osteoarthritis." Thesis, University of Oxford, 2004. https://ora.ox.ac.uk/objects/uuid:1529aa12-2d57-48fd-8cb9-a5bab0291116.

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Osteoarthritis (OA) is a common, disabling disease of the elderly. It is uncommon in individuals under 45 years of age, and prevalence is higher in females than males. OA is characterised by focal cartilage loss accompanied by osteophytes, sclerosis and often some inflammatory response. A significant genetic component to OA has been demonstrated by twin-pair, sibling risk and segregation studies. Our group previously carried out a two-stage genome-wide linkage scan and identified six chromosomal regions as potentially harbouring OA susceptibility genes. The aim of this project was to examine t
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Alldinger, Ingo, Dag Dittert, Matthias Peiper, et al. "Gene expression analysis of pancreatic cell lines reveals genes overexpressed in pancreatic cancer." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-136495.

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Background: Pancreatic cancer is one of the leading causes of cancer-related death. Using DNA gene expression analysis based on a custom made Affymetrix cancer array, we investigated the expression pattern of both primary and established pancreatic carcinoma cell lines. Methods: We analyzed the gene expression of 5 established pancreatic cancer cell lines (AsPC-1, BxPC-3, Capan-1, Capan-2 and HPAF II) and 5 primary isolates, 1 of them derived from benign pancreatic duct cells. Results: Out of 1,540 genes which were expressed in at least 3 experiments, we found 122 genes upregulated and 18 down
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Alldinger, Ingo, Dag Dittert, Matthias Peiper, et al. "Gene expression analysis of pancreatic cell lines reveals genes overexpressed in pancreatic cancer." Karger, 2005. https://tud.qucosa.de/id/qucosa%3A27709.

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Background: Pancreatic cancer is one of the leading causes of cancer-related death. Using DNA gene expression analysis based on a custom made Affymetrix cancer array, we investigated the expression pattern of both primary and established pancreatic carcinoma cell lines. Methods: We analyzed the gene expression of 5 established pancreatic cancer cell lines (AsPC-1, BxPC-3, Capan-1, Capan-2 and HPAF II) and 5 primary isolates, 1 of them derived from benign pancreatic duct cells. Results: Out of 1,540 genes which were expressed in at least 3 experiments, we found 122 genes upregulated and 18 down
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Munye, M. M. "Towards gene therapy for primary ciliary dyskinesia." Thesis, University College London (University of London), 2014. http://discovery.ucl.ac.uk/1435816/.

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Primary ciliary dyskinesia is a genetic disorder where patients develop lung disease as they are unable to clear airway infections effectively. There is currently no treatment for the underlying genetic defect. This thesis describes advances towards the development of a gene therapy targeting the airway disease in PCD patients with DNAH5 mutations, the most common cause of PCD. Little work has been done in the field so many challenges remain. DNAH5 is a large gene and the cDNA has not been cloned. In addition PCD models are currently inadequate. Finally, non-viral vectors for airway gene deliv
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Books on the topic "Gene primers"

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Cenkçi, Burhan. Gene mi Demirel? Sentez Reklamcılık, 1991.

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Culver, Kenneth W. Gene therapy: A primer for physicians. 2nd ed. Mary Ann Liebert, Inc., 1996.

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Purzner, Andrew. Differential gene expression of LUCA-15 and other cancer related genes in primary breast tissue. Laurentian University, 2003.

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H, Schierup Mikkel, and Wiuf Carsten, eds. Gene genealogies, variation and evolution: A primer in coalescent theory. Oxford University Press, 2005.

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Lakshmipathy, Uma. Primary and stem cells: Gene transfer technologies and applications. John Wiley and Sons, 2011.

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Huhtala, Pirkko. Type IV collagenases: Primary structure, genomic structure and gene expression. University of Oulu], Biocenter and Department of Biochemistry, 1991.

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M, Malacinski George, Simonsen Christian C, and Shepard Michael, eds. Molecular genetics of mammalian cells: A primer in developmental biology. Macmillan, 1986.

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Ponce, Claudia Ortega. Relaciones sociales y de genes: El primer vegetal transgénico mexicano. Universidad Autónoma del Estado de México, Facultad de Ciencias Políticas y Sociales, 2010.

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Rabin, Lea. Ich gehe weiter auf seinem Weg: Erinnerungen an Jitzchak Rabin. Droemer Knaur, 1997.

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Arnaout, Angel. An analysis of estrogen receptor alpha gene and protein expression in primary human breast cancers. National Library of Canada, 2003.

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Book chapters on the topic "Gene primers"

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Delgado, Javier A., Steven Meinhardt, Samuel G. Markell, and Rubella S. Goswami. "Gene Cloning Using Degenerate Primers and Genome Walking." In Plant Fungal Pathogens. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-501-5_39.

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Lang, Michael, and Virginie Orgogozo. "Identification of Homologous Gene Sequences by PCR with Degenerate Primers." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-228-1_14.

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Preston, Gregory M. "Cloning Gene Family Members Using PCR with Degenerate Oligonucleotide Primers." In PCR Protocols. Humana Press, 2003. http://dx.doi.org/10.1007/978-1-4612-0055-0_67.

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Borman, Andrew M., and Elizabeth M. Johnson. "Sequence-based Identification and Classification of Fungi." In Trends in the systematics of bacteria and fungi. CABI, 2021. http://dx.doi.org/10.1079/9781789244984.0198.

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Abstract This book chapter describes the advantages and limitations of the ITS Region as a universal barcode for fungal identification. The ITS region offers several practical advantages as a universal fungal barcode region. The region encompasses segments that permit resolution at different taxonomic levels as it includes the highly conserved 5.8S rRNA gene, the moderately rapidly evolving ITS2 region and the rapidly evolving ITS1 region, flanked by the highly conserved SSU and LSU genes which permit design of PCR primers that are almost panfungal. Over the last two decades the sequence-based
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Sachman-Ruiz, Bernardo, Bruno Contreras-Moreira, Enrique Zozaya, Cristina Martínez-Garza, and Pablo Vinuesa. "Primers4clades: A Web Server to Design Lineage-Specific PCR Primers for Gene-Targeted Metagenomics." In Handbook of Molecular Microbial Ecology I. John Wiley & Sons, Inc., 2011. http://dx.doi.org/10.1002/9781118010518.ch51.

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Pierce, Kenneth E., and Lawrence J. Wangh. "Low-Concentration Initiator Primers Improve the Amplification of Gene Targets with High Sequence Variability." In Methods in Molecular Biology. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2365-6_5.

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Saptaningtyas, Ragil, Stalis Norma Ethica, Chaerul Arham, et al. "Listeria monocytogenes Specific Detection Employing Novel Primers with In Silico Study to Detect Listeriolysin O Protein-Coding Gene hly." In Advances in Biological Sciences Research. Atlantis Press International BV, 2024. http://dx.doi.org/10.2991/978-94-6463-457-0_6.

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Dillon, Lawrence S. "Processing the Primary Transcripts." In The Gene. Springer US, 1987. http://dx.doi.org/10.1007/978-1-4899-2007-2_12.

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Fox, Tom, Claire Booth, and Emma C. Morris. "Gene Therapy in Cellular Immunodeficiencies." In Cellular Primary Immunodeficiencies. Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-70107-9_19.

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Gaudet, Pascale, Nives Škunca, James C. Hu, and Christophe Dessimoz. "Primer on the Gene Ontology." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3743-1_3.

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Conference papers on the topic "Gene primers"

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Fida, Tekle, Luke Austin, Scott Leleika, Taylor Rambo, and Karen Crippen. "Development of Molecular Probe for Iron Reducing and Thiosulfate Reducing Bacteria." In CONFERENCE 2024. AMPP, 2024. https://doi.org/10.5006/c2024-20885.

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Abstract Microbiologically Influenced Corrosion (MIC) activities are mostly monitored by using canonical culture-based methods. However, these methods detect only culturable bacteria or require months of incubation to get results. To overcome this, molecular detection methods were developed for many of the MIC-causing microbes but were not available for Iron Reducing Bacteria (IRB) and Thiosulfate Reducing Bacteria (TRB). IRB and TRB are among the dominant group of microbes involved in corrosion and souring oil and gas infrastructures. In the present study, molecular probes were developed for
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Le Borgne, S., J. M. Romero, H. A. Videla, J. M. Gonzalez, and C. Saiz-Jiménez. "Practical Cases of the Use of Molecular Techniques to Characterize Microbial Deterioration of Metallic Structures in Industry." In CORROSION 2007. NACE International, 2007. https://doi.org/10.5006/c2007-07523.

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Abstract Two specific cases of applying molecular techniques for deciphering the role of microorganisms in industrial processes are presented: an offshore seawater injection system and a wastewater treatment plant. In the first case, deoxyribonucleic acid (DNA) was extracted from a water sample taken from an offshore seawater injection system and from enrichment cultures from the same sample. The V3 hypervariable region of the 16S rDNA gene was amplified by the polymerase chain reaction (PCR) and bacterial diversity was studied using denaturing gel gradient electrophoresis (DGGE). DGGE monitor
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Manna, Kathleen, Joseph Moore, Kenneth Wunch, et al. "Relative Performance of Various 16S iTag Amplicon Sequencing Primer Pairs in Profiling Microbial Communities Relevant to Oil & Gas Operations." In CORROSION 2019. NACE International, 2019. https://doi.org/10.5006/c2019-13442.

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Abstract The oil and gas industry's efforts to characterize microbial communities in oilfield process fluids has shed increasing light on the influence of bacteria and archaea on hydrocarbon production. Notably, topside or downhole water contamination by microorganisms such as sulfate-reducing bacteria, acid-producing bacteria, and/or other halophiles can negatively impact asset integrity and reduce the quality and quantity of produced hydrocarbons. Molecular microbiology methods have contributed to our understanding of these complex processes by providing unprecedented resolution of resident
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Баженов, М. С. "AUTOMATED SELECTION OF SPECIFIC PRIMERS FOR GENE CLONING IN COMMON WHEAT." In Биотехнология в растениеводстве, животноводстве и сельскохозяйственной микробиологии. Crossref, 2022. http://dx.doi.org/10.48397/arriab.2022.22.xxii.001.

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Клонирование и секвенирование отдельных генов пшеницы остается актуальным методом изучения её наследственного разнообразия, позволяющим находить новые ценные мутации, в том числе, получаемые современными методами генного редактирования. Подбор праймеров для аллополиплоидных видов, таких как пшеница мягкая (Triticum aestivum L.), осложняется сходством нуклеотидных последовательностей их субгеномов. Cloning and sequencing of individual wheat genes remains a relevant method for studying its hereditary diversity, which makes it possible to find new valuable mutations, including those obtained by m
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Dalpiaz, Giovana, NATASHA MALGAREZI, MARIANA ROST MEIRELES, and PRISCILA LORA. "DESENHO E PADRONIZAÇÃO DE PRIMERS PARA DETECÇÃO DE GENES DE RESISTÊNCIA À CARBAPENÊMICOS ENGLOBANDO MAIS DE 200 VARIANTES." In II Congresso Nacional de Microbiologia Clínica On-line. Revista Multidisciplinar em Saúde, 2022. http://dx.doi.org/10.51161/ii-conamic/17.

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Introdução: A resistência bacteriana aos antibióticos carbapenêmicos corresponde atualmente a uma grande preocupação para a saúde pública, por estar associada a uma maior taxa de mortalidade e morbidade. A detecção precoce da resistência bacteriana a estes antibióticos diminui o tempo de hospitalização bem como complicações decorrentes de infecções. A associação de técnicas de biologia molecular e microbiologia tem potencial para agilizar e qualificar o diagnóstico clínico de resistência. Objetivo: O objetivo do presente estudo foi estabelecer primers para Polymerase chain reaction (PCR) que d
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Moura, Maria Eduarda Gobara de, Laís Stella Perassoli Nicchio, and Cristiane Suemi Shinobu Mesquita. "CONFECÇÃO DE PRIMERS PARA O GENE DA METALOPROTEINASE - 2 (MMP-2) PARA REALIZAÇÃO DE METODOLOGIA DE PCR EM TEMPO REAL." In I Congresso Nacional Multidisciplinar de Oncologia On-line. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1557.

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Introdução: O câncer de mama (CM) é o segundo tipo de câncer mais frequente entre as mulheres, sendo responsável por 66.280 novos casos no ano passado. Biomarcadores são parâmetros biológicos que refletem desequilíbrios moleculares provenientes de patologias, colaborando com exames laboratoriais. A metaloproteinase de matriz 2 (MMP-2) é uma enzima que digere a matriz extracelular e responde à alterações moleculares, sendo que em células cancerígenas sua expressão é aumentada. Sua presença no soro e tecido de pacientes com câncer mamário pode indicar invasão tumoral. Objetivos: Confeccionar pri
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Zubanova, Yu S., V. A. Filobok, E. A. Guenkova, E. R. Davoyan, D. M. Boldakov, and D. S. Mikov. "Identification of allelic combinations of the Ppd-D1, Vrn-A1, Vrn-B1 and Vrn-D1 genes in common wheat lines obtained in the National Center of Grain named after P. P. Lukyanenko." In CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-2020-5-9-10-63.

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An analysis of the allelic composition of the genes determining photoperiodic sensitivity (Ppd-D1) and the need for vernalization (Vrn-A1, Vrn-B1, Vrn-D1) was carried out in 286 common wheat lines obtained in the National Center of Grain named after P. P. Lukyanenko with the use of allele-specific primers. The analyzed samples were distributed over 21 haplotypes; the dominant allele of the Ppd-D1a gene prevailed in the studied material. 123 lines of common wheat carry a combination of D-RRD alleles. The lines that can be attributed to the group of alternate wheat (R-RDR, R-RRD) were identified
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Moraes, Natasha Malgarezi De, Giovana Dalpiaz, Mariana Rost Meireles, and Priscila Schmidt Lora. "DESENHO E AVALIAÇÃO DA SENSIBILIDADE DE UM PRIMER PARA DETECÇÃO DO GENE BLANDM DE RESISTÊNCIA BACTERIANA À CARBAPENÊMICOS." In II Congresso Brasileiro de Biotecnologia On-line. Revista Multidisciplinar de Educação e Meio Ambiente, 2022. http://dx.doi.org/10.51189/conbiotec/46.

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Introdução: A resistência bacteriana a antibióticos é um problema de saúde pública que eleva a mortalidade e os custos para o sistema de saúde. O diagnóstico laboratorial microbiológico requer etapas de crescimento bacteriano em cultura por pelo menos 24h para realização da análise adequada, atrasando e limitando as opções de tratamento. O uso da biologia molecular como forma de diagnóstico confere um grau de inovação tecnológica que pode aumentar a sensibilidade e diminuir o tempo necessário ao diagnóstico. Objetivo: O objetivo do trabalho foi estabelecer um primer que fosse capaz de amplific
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Khamwan, Sumolnat, and Wiyada Mongkolthanaruk. "Exploring of the inulin synthesis gene of endophytic bacteria by the new degenerated primers." In Annual International Conference on Advances in Biotechnology (BIOTECH 2016). Global Science & Technology Forum (GSTF), 2016. http://dx.doi.org/10.5176/2251-2489_biotech16.13.

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Kadir, Zaiton Abdul, Fauzi Daud, Azhar Mohamad, Sahidan Senafi та Ferlynda Fazleen Jamaludin. "Optimization of β-glucan synthase gene primers for molecular DNA fingerprinting in Pleurotus pulmonarious". У THE 2015 UKM FST POSTGRADUATE COLLOQUIUM: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. AIP Publishing LLC, 2015. http://dx.doi.org/10.1063/1.4931252.

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Reports on the topic "Gene primers"

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Patarakul, Kanitha, and Yong Poovoravan. Study of homology of mce and invA genes among different serovars of Leptospira interrogans and their in vivo expression. Faculty of Medicine, Chulalongkorn University, 2010. https://doi.org/10.58837/chula.res.2010.13.

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Leptospirosis is a worldwide zoonotic disease. Pathogenesis of leptospirosis is not well understood. Identification of virulence factors of pathogenic Leptospira and their roles in pathogenesis is crucial. Based on available whole-genome sequences of Leptospira, two presumptive virulence genes which are homologous to the invasionA (invA) gene of Rickettsia prowazekii and the mammalian cell entry (mce) gene of Mycobacterium tuberculosis, have been identified. The function of these genes may involve in the attachment and invasion of eukaryotic cells. We proposed that these gene homologues should
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Amzeri, Achmad, Kaswan Badami, and Gita Pawana. Inheritance of resistance to downy mildew (Peronosclerospora maydis) in crossing of Madura Maize Plant (Zea mays L.). Innovative Scientific Information & Services Network, 2019. http://dx.doi.org/10.21107/amzeri.2019.1.

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Hybridization of Back cross is one method to get varieties that are resistant to downy mildew. The purpose of this study was to obtain information on inheritance characteristics of downy mildew resistance. This research was conducted at the experiment center of Agro-Technology Study Program of Agriculture Faculty, University of Trunojoyo Madura. Research of Assessment of resistance to Downy Mildew used a randomized block design with 18 treatments (P1, P2, F1, F2, BC1P1 and BC1P2 in three sets of crosses, namely LGL x Mdr-3, T12 x Mdr-1 and E02 x Mdr-2) and three replications so there were 54 e
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Gal-On, Amit, Shou-Wei Ding, Victor P. Gaba, and Harry S. Paris. role of RNA-dependent RNA polymerase 1 in plant virus defense. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7597919.bard.

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Objectives: Our BARD proposal on the impact of RNA-dependent RNA polymerase 1 (RDR1) in plant defense against viruses was divided into four original objectives. 1. To examine whether a high level of dsRNA expression can stimulate RDR1 transcription independent of salicylic acid (SA) concentration. 2. To determine whether the high or low level of RDR1 transcript accumulation observed in virus resistant and susceptible cultivars is associated with viral resistance and susceptibility. 3. To define the biogenesis and function of RDR1-dependent endogenous siRNAs. 4. To understand why Cucumber mosai
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Zchori-Fein, Einat, Judith K. Brown, and Nurit Katzir. Biocomplexity and Selective modulation of whitefly symbiotic composition. United States Department of Agriculture, 2006. http://dx.doi.org/10.32747/2006.7591733.bard.

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Whiteflies are sap-sucking insects that harbor obligatory symbiotic bacteria to fulfill their dietary needs, as well as a facultative microbial community with diverse bacterial species. The sweetpotato whitefly Bemisia tabaci (Gennadius) is a severe agricultural pest in many parts of the world. This speciesconsists of several biotypes that have been distinguished largely on the basis of biochemical or molecular diagnostics, but whose biological significance is still unclear. The original objectives of the project were (i) to identify the specific complement of prokaryotic endosymbionts associa
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Heifetz, Yael, and Michael Bender. Success and failure in insect fertilization and reproduction - the role of the female accessory glands. United States Department of Agriculture, 2006. http://dx.doi.org/10.32747/2006.7695586.bard.

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The research problem. Understanding of insect reproduction has been critical to the design of insect pest control strategies including disruptions of mate-finding, courtship and sperm transfer by male insects. It is well known that males transfer proteins to females during mating that profoundly affect female reproductive physiology, but little is known about the molecular basis of female mating response and no attempts have yet been made to interfere with female post-mating responses that directly bear on the efficacy of fertilization. The female reproductive tract provides a crucial environm
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Eshed, Y., and Z. B. Lippman. Fine tuning the shoot and inflorescence architectures for improved tomato yield. United States-Israel Binational Agricultural Research and Development Fund, 2022. http://dx.doi.org/10.32747/2022.8134148.bard.

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In this project, we are determining the contribution of different types of variations, in gene function and in gene regulation, to altered shoot architecture first, and to field performance in the next stage. We are using tomato as a target, but also as a model for many other crops. Our focus is on two different components of yield associated traits - shoot architecture and organization of the inflorescence. Our focus was on two types of regulators; 1) genes involved in florigen - antiflogen balance and the way they impact the shoot, and 2) genes involved in inflorescence branching and it this
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Reisch, Bruce, Avichai Perl, Julie Kikkert, Ruth Ben-Arie, and Rachel Gollop. Use of Anti-Fungal Gene Synergisms for Improved Foliar and Fruit Disease Tolerance in Transgenic Grapes. United States Department of Agriculture, 2002. http://dx.doi.org/10.32747/2002.7575292.bard.

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Original objectives . 1. Test anti-fungal gene products for activity against Uncinula necator, Aspergillus niger, Rhizopus stolonifer and Botrytis cinerea. 2. For Agrobacterium transformation, design appropriate vectors with gene combinations. 3. Use biolistic bombardment and Agrobacterium for transformation of important cultivars. 4. Characterize gene expression in transformants, as well as level of powdery mildew and Botrytis resistance in foliage of transformed plants. Background The production of new grape cultivars by conventional breeding is a complex and time-consuming process. Transfer
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Grumet, Rebecca, Rafael Perl-Treves, and Jack Staub. Ethylene Mediated Regulation of Cucumis Reproduction - from Sex Expression to Fruit Set. United States Department of Agriculture, 2010. http://dx.doi.org/10.32747/2010.7696533.bard.

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Reproductive development is a critical determinant of agricultural yield. For species with unisexual flowers, floral secualdifferentation adds additional complexity, that can influenec productivity. The hormone ethylene has long, been known to play a primary role in sex determination in the Cucumis species cucumber (C. sativus) and melon (C. melo). Our objectives were to: (1) Determine critical sites of ethylene production and perception for sex determination; (2) Identify additional ethylene related genes associated with sex expression; and (3) Examine the role of environment ami prior fruit
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Mawassi, Munir, Baozhong Meng, and Lorne Stobbs. Development of Virus Induced Gene Silencing Tools for Functional Genomics in Grapevine. United States Department of Agriculture, 2013. http://dx.doi.org/10.32747/2013.7613887.bard.

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Grapevine is perhaps the most widely grown fruit crop. To understand the genetic make-up so as to improve the yield and quality of grapes and grape products, researchers in Europe have recently sequenced the genomes of Pinot noir and its inbred. As expected, function of many grape genes is unknown. Functional genomics studies have become the major focus of grape researchers and breeders. Current genetic approaches for gene function studies include mutagenesis, crossing and genetic transformation. However, these approaches are difficult to apply to grapes and takes long periods of time to accom
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Or, Etti, Tai-Ping Sun, Amnon Lichter, and Avichai Perl. Characterization and Manipulation of the Primary Components in Gibberellin Signaling in the Grape Berry. United States Department of Agriculture, 2010. http://dx.doi.org/10.32747/2010.7592649.bard.

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Seedless cultivars dominate the table grape industry. In these cultivars it is mandatory to apply gibberellin (GA) to stimulate berry development to a commercially acceptable size. These cultivars differ in their sensitivity to GA application, and it frequently results in adverse effects such as decreased bud fertility and increased fruit drop. Our long term goals are to (1) understand the molecular basis for the differential sensitivity and identify markers for selection of sensitive cultivars (2) to develop new strategies for targeted manipulation of the grape berry response to GA that will
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