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Journal articles on the topic 'Generator of markers'

Author: Grafiati

Published: 4 June 2021

Last updated: 16 February 2022

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1

Martínez-Pastor, Felipe, Eduardo Aisen, María Rocío Fernández-Santos, et al. "Reactive oxygen species generators affect quality parameters and apoptosis markers differently in red deer spermatozoa." REPRODUCTION 137, no. 2 (2009): 225–35. http://dx.doi.org/10.1530/rep-08-0357.

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Fe2+/ascorbate, hydrogen peroxide (H2O2), and hypoxanthine/xanthine oxidase (XOD) are commonly used for inducing oxidative stress on spermatozoa. A comparative study of these agents was carried out on thawed spermatozoa from red deer. First, we tested a high, medium, and low concentration of each agent: 100, 10, and 1 μM Fe2+ (hydroxyl radical generator); 1 mM, 100, and 10 μM H2O2; and 100, 10, and 1 mU/ml XOD (superoxide and H2O2 generator), incubated at 37 °C for 180 min. Intracellular reactive oxygen species (ROS; H2DCFDA) increased with dose and time similarly for the three systems at each

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Pryanichnikov, Valentin, Egor Shipovalov, and Radomir Tarasov. "Intelligent support of logistics missions in medical institutions by mobile service robots AMUR-307." Robotics and Technical Cybernetics 9, no. 2 (2021): 121–26. http://dx.doi.org/10.31776/rtcj.9206.

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Technologies of missions’ generation for the mobile robots (MR) Amur-307 by the parallelized automatic scheduler and their implementation by the integration software (SW) with the hardware block (HB) on 6 power channels (6x30A), with the branched sensorics are considered. The program generator is forming the tasks in the PDDL language based on the route map in vector graphic format, parameters of objects moved by robots, and equipment installed on robots. The developed plan is implemented by the HB and the navigation control program, based on topological information extracted from the map, bas

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Piña-Ramirez, Omar, Raquel Valdes-Cristerna, and Oscar Yanez-Suarez. "Scenario Screen: A Dynamic and Context Dependent P300 Stimulator Screen Aimed at Wheelchair Navigation Control." Computational and Mathematical Methods in Medicine 2018 (2018): 1–16. http://dx.doi.org/10.1155/2018/7108906.

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P300 spellers have been widely modified to implement nonspelling tasks. In this work, we propose a “scenario” stimulation screen that is a P300 speller variation to command a wheelchair. Our approach utilized a stimulation screen with an image background (scenario snapshot for a wheelchair) and stimulation markers arranged asymmetrically over relevant landmarks, such as suitable paths, doors, windows, and wall signs. Other scenario stimulation screen features were green/blue stimulation marker color scheme, variable Interstimulus Interval, single marker stimulus mode, and optimized stimulus se

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Mathema, Vivek Bhakta, Arjen M. Dondorp, and Mallika Imwong. "OSTRFPD: Multifunctional Tool for Genome-Wide Short Tandem Repeat Analysis for DNA, Transcripts, and Amino Acid Sequences with Integrated Primer Designer." Evolutionary Bioinformatics 15 (January 2019): 117693431984313. http://dx.doi.org/10.1177/1176934319843130.

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Microsatellite mining is a common outcome of the in silico approach to genomic studies. The resulting short tandemly repeated DNA could be used as molecular markers for studying polymorphism, genotyping and forensics. The omni short tandem repeat finder and primer designer (OSTRFPD) is among the few versatile, platform-independent open-source tools written in Python that enables researchers to identify and analyse genome-wide short tandem repeats in both nucleic acids and protein sequences. OSTRFPD is designed to run either in a user-friendly fully featured graphical interface or in a command

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Chiu, Sung Kay, Ming Hua Hsieh, and Chi Meng Tzeng. "Unique marker finder algorithm generates molecular diagnostic markers." International Journal of Bioinformatics Research and Applications 7, no. 1 (2011): 24. http://dx.doi.org/10.1504/ijbra.2011.039168.

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Dembogurski, Renan Augusto, Bruno José Dembogurski, José Luiz Ribeiro De Souza Filho, Dhiego Oliveira Sad, Rodrigo De Souza Silva, and Marcelo Bernardes Vieira. "Interactive Virtual Terrain Generation using Augmented Reality Markers." Journal on Interactive Systems 3, no. 3 (2013): 1. http://dx.doi.org/10.5753/jis.2012.619.

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This paper presents an application that allows the generation of virtual terrains interactively, using augmented reality markers. This application also allows the user to navigate in the generated virtual environment. To demonstrate how the process is done, a terrain generation scenario was chosen. Virtual objects were augmented using markers and the detection is done through the ARToolKit framework. A particle system was used to simulate deformation to better incorporate the needs of terrain generation. The deformation itself follows an interparticle force between the particles attached to a

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Hubmayr, R. D., J. Sprung, and S. Nelson. "Determinants of transdiaphragmatic pressure in dogs." Journal of Applied Physiology 69, no. 6 (1990): 2050–56. http://dx.doi.org/10.1152/jappl.1990.69.6.2050.

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We measured the transdiaphragmatic pressure (Pdi) during bilateral phrenic nerve stimulation and evaluated the determinants of its change with lung volume, chest wall geometry, and respiratory system impedance in supine dogs. Four rows of radiopaque markers were sewn onto muscle bundles of the costal and crural diaphragm between their origin on the central tendon and their insertion on the rib cage and spine. The length of the diaphragm (L) was determined from the projection images of marker rows using biplane fluoroscopy. Measurements were made at lung volumes between total lung capacity and

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Díaz-Ríos, Manuel, and Mark W. Miller. "Rapid Dopaminergic Signaling by Interneurons That Contain Markers for Catecholamines and GABA in the Feeding Circuitry of Aplysia." Journal of Neurophysiology 93, no. 4 (2005): 2142–56. http://dx.doi.org/10.1152/jn.00003.2004.

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Consummatory feeding behaviors in Aplysia californica are controlled by a polymorphic central pattern generator (CPG) circuit. Previous investigations have demonstrated colocalization of markers for GABA and catecholamines within two interneurons, B20 and B65, that participate in configuring the functional output of this CPG. This study examined the contributions of GABA and dopamine (DA) to rapid synaptic signaling from B20 and B65 to follower cells that implement their specification of motor programs. Pharmacological tests did not substantiate the participation of GABA in the mediation of th

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Gürkov, R., L. B. Filipe Mingas, T. Rader, J. Louza, B. Olzowy, and E. Krause. "Effect of transtympanic low-pressure therapy in patients with unilateral Menière's disease unresponsive to betahistine: a randomised, placebo-controlled, double-blinded, clinical trial." Journal of Laryngology & Otology 126, no. 4 (2012): 356–62. http://dx.doi.org/10.1017/s0022215112000102.

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AbstractObjective:To determine the effect of the Meniett® low-pressure generator on the subjective symptoms and audiovestibular disease markers of patients with unilateral Menière's disease unresponsive to betahistine treatment.Methods:Randomised, placebo-controlled, double-blinded, clinical trial at a tertiary referral centre. After ventilation tube placement, patients were randomised to the active treatment or placebo group. Monitoring comprised audiometry and air caloric testing and a vertigo diary (enabling calculation of vertigo and activity scores, and the number of vertigo days, vertigo

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Greybeck, Brooke, Raymond Lu, Arvind Ramanujam, et al. "Regional diaphragm volume displacement is heterogeneous in dogs." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 312, no. 3 (2017): R443—R450. http://dx.doi.org/10.1152/ajpregu.00270.2016.

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Muscle shortening and volume displacement (VD) are critical determinants of the pressure-generating capacity of the diaphragm. The present study was designed to test the hypothesis that diaphragm VD is heterogeneous and that distribution of VD is dependent on regional muscle shortening, posture, and the level of muscle activation. Radioopaque markers were sutured along muscle bundles of the peritoneal surface of the crural, dorsal costal, midcostal, and ventral costal regions of the left hemidiaphragm in four dogs. The markers were followed by biplanar video fluoroscopy during quiet spontaneou

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Tanhuanpää, Pirjo, Ruslan Kalendar, Jaana Laurila, Alan H. Schulman, Outi Manninen, and Elina Kiviharju. "Generation of SNP markers for short straw in oat (Avena sativa L.)." Genome 49, no. 3 (2006): 282–87. http://dx.doi.org/10.1139/g05-100.

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Short straw is a desired trait in oat germplasm (Avena sativa L.). Marker-assisted selection, a key tool for achieving this objective, is limited by the presence and number of available markers. Here, we have attempted to develop markers sufficiently linked to a gene specifying short straw so that marker-assisted selection could be applied. Bulked-segregant analysis was used to identify anonymous PCR-based markers associated with the dwarfing gene Dw6 in an F2 population from the cross between A. sativa 'Aslak' and A. sativa 'Kontant'. One random amplified polymorphic DNA (RAPD) and 1 retrotra

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Crawford, A. M., K. G. Dodds, A. J. Ede, et al. "An autosomal genetic linkage map of the sheep genome." Genetics 140, no. 2 (1995): 703–24. http://dx.doi.org/10.1093/genetics/140.2.703.

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Abstract We report the first extensive ovine genetic linkage map covering 2070 cM of the sheep genome. The map was generated from the linkage analysis of 246 polymorphic markers, in nine three-generation full-sib pedigrees, which make up the AgResearch International Mapping Flock. We have exploited many markers from cattle so that valuable comparisons between these two ruminant linkage maps can be made. The markers, used in the segregation analyses, comprised 86 anonymous microsatellite markers derived from the sheep genome, 126 anonymous microsatellites from cattle, one from deer, and 33 poly

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ZHAO, H., D. NETTLETON, M. SOLLER, and J. C. M. DEKKERS. "Evaluation of linkage disequilibrium measures between multi-allelic markers as predictors of linkage disequilibrium between markers and QTL." Genetical Research 86, no. 1 (2005): 77–87. http://dx.doi.org/10.1017/s001667230500769x.

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Effectiveness of marker-assisted selection (MAS) and quantitative trait loci (QTL) mapping using population-wide linkage disequilibrium (LD) between markers and QTL depends on the extent of LD and how it declines with distance in a population. Because marker–QTL LD cannot be observed directly, the objective of this study was to evaluate alternative measures of observable LD between multi-allelic markers as predictors of usable LD of multi-allelic markers with presumed biallelic QTL. Observable LD between marker pairs was evaluated using eight existing measures and one new measure. These consis

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Collard, Bertrand, Emma Mace, Mark McPhail, et al. "How accurate are the marker orders in crop linkage maps generated from large marker datasets?" Crop and Pasture Science 60, no. 4 (2009): 362. http://dx.doi.org/10.1071/cp08099.

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Marker ordering during linkage map construction is a critical component of QTL mapping research. In recent years, high-throughput genotyping methods have become widely used, and these methods may generate hundreds of markers for a single mapping population. This poses problems for linkage analysis software because the number of possible marker orders increases exponentially as the number of markers increases. In this paper, we tested the accuracy of linkage analyses on simulated recombinant inbred line data using the commonly used Map Manager QTX (Manly et al. 2001: Mammalian Genome 12, 930–93

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Maleev, D., A. Isaev, Ju Petrova, V. Zalyapin, A. Shevtsov, and Yu Korableva. "INTEGRAL ASSESSMENT OF BODY RESERVES IN SKI RACERS DEVELOPING LOCAL REGIONAL MUSCLE ENDURANCE, STATOKINETIC AND HYPOXIA RESISTANCE." Human Sport Medicine 20, no. 1 (2020): 43–51. http://dx.doi.org/10.14529/hsm200106.

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Aim. The article aims to assess the body reserves of ski racers who develop local-regional muscle endurance (LRME), statokinetic and hypoxia resistance. Materials and methods. 15–16-year-old highly skilled ski-racers (I, II category, candidates for master of sports) with sports experience of 5–7 years (n = 12) were examined. The following equipment was used to assess athletic fitness and sports performance: T 2100 GE treadmill system, CardioSoft diagnostic system (USA), HYPOXICO Everest Summit II altitude generator (USA), Angio Scan-01 P pulse oximeter sensor (Russia). Statistical analysis was

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Di Stefano, Antonino, Roberto Frairia, Fabio L. M. Ricciardolo, et al. "Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients." Canadian Respiratory Journal 2020 (August 8, 2020): 1–14. http://dx.doi.org/10.1155/2020/1524716.

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Chronic obstructive pulmonary disease (COPD) is due to structural changes and narrowing of small airways and parenchymal destruction (loss of the alveolar attachment as a result of pulmonary emphysema), which all lead to airflow limitation. Extracorporeal shock waves (ESW) increase cell proliferation and differentiation of connective tissue fibroblasts. To date no studies are available on ESW treatment of human bronchial fibroblasts and epithelial cells from COPD and control subjects. We obtained primary bronchial fibroblasts from bronchial biopsies of 3 patients with mild/moderate COPD and 3

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Guo, Baozhu, Manish K. Pandey, Guohao He, et al. "Recent Advances in Molecular Genetic Linkage Maps of Cultivated Peanut." Peanut Science 40, no. 2 (2013): 95–106. http://dx.doi.org/10.3146/ps13-03.1.

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ABSTRACT The competitiveness of peanuts in domestic and global markets has been threatened by losses in productivity and quality that are attributed to diseases, pests, environmental stresses and allergy or food safety issues. Narrow genetic diversity and a deficiency of polymorphic DNA markers severely hindered construction of dense genetic maps and quantitative trait loci (QTL) mapping in order to deploy linked markers in marker-assisted peanut improvement. The U.S. Peanut Genome Initiative (PGI) was launched in 2004, and expanded to a global effort in 2006 to address these issues through co

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McGrath, J. Mitchell, and Carlos F. Quiros. "Generation of alien chromosome addition lines from synthetic Brassica napus: morphology, cytology, fertility, and chromosome transmission." Genome 33, no. 3 (1990): 374–83. http://dx.doi.org/10.1139/g90-057.

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The chromosome complement of Brassica oleracea (2n = 18) was dissected by means of alien chromosome addition lines generated by successive backcrosses of either of two B. campestris accessions (2n = 20) to the resynthesized B. napus 'Hakuran' (2n = 38). Alien chromosome addition lines were characterized by chromosome counts, morphology, pollen and seed fertility, and transmission of chromosome-specific markers. Mean chromosome number in the first backcross generation was approximately 23.5 and was little influenced by the B. campestris accession. Fertility and isozyme marker transmission were

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Nadworny, Alyson S., Mallik R. Guruju, Daniel Poor, et al. "Nox2 and Nox4 influence neonatal c-kit+ cardiac precursor cell status and differentiation." American Journal of Physiology-Heart and Circulatory Physiology 305, no. 6 (2013): H829—H842. http://dx.doi.org/10.1152/ajpheart.00761.2012.

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Redox status has emerged as critical in modulating stemness and lineage commitment in several precursor cell types. However, a role for redox genes, specifically NADPH oxidases (Nox), in cardiac precursor cells (CPCs) has not been established. We tested whether CPCs marked by type III receptor tyrosine kinase c-kit (c-kit+) exhibit a unique NADPH oxidase signature that confers precursor status and whether alterations in this profile are functionally linked to changes in lineage specification. Dihydroethidium (DHE) microfluorography indicated reduced basal reactive oxygen species (ROS) formatio

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Ahn, Yul-Kyun, Swati Tripathi, Young-Il Cho, et al. "Molecular marker information from de novo assembled transcriptomes of chilli pepper (Capsicum annuum L.) varieties based on next-generation sequencing technology." Plant Genetic Resources 12, S1 (2014): S83—S86. http://dx.doi.org/10.1017/s147926211400032x.

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Next-generation sequencing technique has been known as a useful tool for de novo transcriptome assembly, functional annotation of genes and identification of molecular markers. This study was carried out to mine molecular markers from de novo assembled transcriptomes of four chilli pepper varieties, the highly pungent ‘Saengryeg 211’ and non-pungent ‘Saengryeg 213’ and variably pigmented ‘Mandarin’ and ‘Blackcluster’. Pyrosequencing of the complementary DNA library resulted in 361,671, 274,269, 279,221, and 316,357 raw reads, which were assembled in 23,607, 19,894, 18,340 and 20,357 contigs, f

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Jun, Tae-Hwan, Andrew P. Michel, and M. A. Rouf Mian. "Development of soybean aphid genomic SSR markers using next generation sequencing." Genome 54, no. 5 (2011): 360–67. http://dx.doi.org/10.1139/g11-002.

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Simple sequence repeats (SSRs) or microsatellites are very useful molecular markers, owing to their locus-specific codominant and multiallelic nature, high abundance in the genome, and high rates of transferability across species. The soybean aphid ( Aphis glycines Matsumura) has become the most damaging insect pest of soybean ( Glycine max (L.) Merr.) in North America, since it was first found in the Midwest of the United States in 2000. Biotypes of the soybean aphid capable of colonizing newly developed aphid-resistant soybean cultivars have been recently discovered. Genetic resources, inclu

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Stahl, Brian, Yufeng Li, Brittany Hermecz, and Stefanie Woodard. "Out With the Old and in With the New? Factors Involved in Migration of Older and Newer Generation Stereotactic Breast Biopsy Markers." Journal of Breast Imaging 3, no. 4 (2021): 465–72. http://dx.doi.org/10.1093/jbi/wbab006.

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Abstract Objective The purpose of this study was to investigate four commonly used stereotactic biopsy markers, two older and two newer generation, assessing percentage migration and factors influencing migration distance. Methods This was an IRB–approved retrospective review of upright stereotactic breast biopsies from May 2018 to May 2020 involving either older (Cork, Hourglass) or newer (Vision, X-shaped) generation markers. Markers were assessed for migration rate by two-sample Z-test and migration distance by analysis of variance. Univariate analysis was used to assess relationships betwe

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Karakousis, A., A. R. Barr, K. J. Chalmers, et al. "Potential of SSR markers for plant breeding and variety identification in Australian barley germplasm." Australian Journal of Agricultural Research 54, no. 12 (2003): 1197. http://dx.doi.org/10.1071/ar02178.

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SSR markers closely linked to 18 loci that control 16 important barley traits were assessed for their applicability in Australian barley breeding programs. A panel of 40 genotypes routinely used by the South Australian Barley Improvement Program (SABIP) was used to examine the usefulness of these SSR markers for marker assisted selection (MAS). The success of monitoring a trait locus from donor to recipient lines ranged from 10 to 98%, depending on the marker. SSRs with a high polymorphic information content (PIC) value were found to be the most useful for application in MAS. The assessment al

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Mokhtari, Reza Bayat, Bessi Qorri, Manpreet Sambi, et al. "3D Multicellular Stem-Like Human Breast Tumor Spheroids Enhance Tumorigenicity of Orthotopic Xenografts in Athymic Nude Rat Model." Cancers 13, no. 11 (2021): 2784. http://dx.doi.org/10.3390/cancers13112784.

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Therapeutic targeting of stem cells needs to be strategically developed to control tumor growth and prevent metastatic burden successfully. Breast cancer presents a unique clinical problem because of the variety of cellular subtypes present, including cancer stem cells (CSCs). The development of 3D stem-like properties of human breast tumor spheroids in stem cell factor conditioned media was investigated in orthotopic xenografts for enhanced tumorgenicity in the athymic nude rat model. MCF-7, ZR-75-1, and MDA-MB-231 breast cancer cell lines were cultured in serum-free, stem cell factor-supplem

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Stalker, H. T., and L. G. Mozingo. "Molecular Markers of Arachis and Marker-Assisted Selection." Peanut Science 28, no. 2 (2001): 117–23. http://dx.doi.org/10.3146/i0095-3679-28-2-13.

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Abstract Many agronomic traits are difficult to select in Arachis hypogaea L. by conventional selection techniques, and marker-assisted selection offers an additional tool for obtaining improved germplasm lines. Molecular markers allow more efficient selection and offer a mechanism to eliminate undesirable traits associated with hybridizing diverse genotypes. The cultivated peanut has been analyzed by several marker systems, including RFLPs, RAPDs, AFLPs, and SSRs. Variation has been observed among diverse genotypes in approximately 5% of the markers analyzed, but the number is much lower betw

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Chatterjee, S. N., and T. P. Mohandas. "Identification of ISSR markers associated with productivity traits in silkworm, Bombyx mori L." Genome 46, no. 3 (2003): 438–47. http://dx.doi.org/10.1139/g03-024.

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Bombyx mori L., commonly recognised around the world as the mulberry silkworm, is characterized by a wide variability in yield and developmental traits, which have been proven through conventional genetic analysis to be of polygenic nature. A large number of morpho-biochemical traits and RFLP and RAPD markers are mapped on different linkage groups, but to this point very little attention has been given to unravelling the genetics of yield traits. To address this issue, polymorphic profiles of 147 markers generated with 12 ISSR primers on the genomic DNA of 20 silkworm stocks of diverse yield s

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Tsitrone, Anne, François Rousset, and Patrice David. "Heterosis, Marker Mutational Processes and Population Inbreeding History." Genetics 159, no. 4 (2001): 1845–59. http://dx.doi.org/10.1093/genetics/159.4.1845.

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AbstractGenotype-fitness correlations (GFC) have previously been studied using allozyme markers and have often focused on short-term processes such as recent inbreeding. Thus, models of GFC usually neglect marker mutation and only use heterozygosity as a genotypic index. Recently, GFC have also been reported (i) with DNA markers such as microsatellites, characterized by high mutation rates and specific mutational processes and (ii) using new individual genotypic indices assumed to be more precise than heterozygosity. The aim of this article is to evaluate the theoretical impact of marker mutat

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Zhao, Jun, Xueqin Tang, Charlene P. Wight, et al. "Genetic mapping and a new PCR-based marker linked to a dwarfing gene in oat (Avena sativa L.)." Genome 61, no. 7 (2018): 497–503. http://dx.doi.org/10.1139/gen-2017-0006.

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Short straw is a desired trait in cultivated hexaploid oat (Avena sativa L.) for some production environments. Marker-assisted selection, a key tool for achieving this objective, is limited by a lack of mapping data and available markers. Here, bulked-segregant analysis was used to identify PCR-based markers associated with a dwarfing gene. Genetic analysis identified a monogenic dominant inheritance of one dwarfing gene from WAOAT2132, temporarily designated DwWA. A simple sequence repeat (SSR) marker (AME117) that was already available and a new codominant PCR-based marker (bi17) developed b

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Xie, Mimi, Yao Ming, Feng Shao, Jianbo Jian, Yaoguang Zhang, and Zuogang Peng. "Restriction site-associated DNA sequencing for SNP discovery and high-density genetic map construction in southern catfish ( Silurus meridionalis )." Royal Society Open Science 5, no. 5 (2018): 172054. http://dx.doi.org/10.1098/rsos.172054.

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Single-nucleotide polymorphism (SNP) markers and high-density genetic maps are important resources for marker-assisted selection, mapping of quantitative trait loci (QTLs) and genome structure analysis. Although linkage maps in certain catfish species have been obtained, high-density maps remain unavailable in the economically important southern catfish ( Silurus meridionalis ). Recently developed restriction site-associated DNA (RAD) markers have proved to be a promising tool for SNP detection and genetic map construction. The objective of the present study was to construct a high-density lin

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Kozak, Bartosz, Renata Galek, Dariusz Zalewski, and Ewa Sawicka-Sienkiewicz. "Preliminary Genetic Map of a New Recombinant Inbred Line Population for Narrow-leafed Lupin (Lupinus angustifolius L.)." Agronomy 9, no. 10 (2019): 653. http://dx.doi.org/10.3390/agronomy9100653.

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Genetic maps are an essential tool for investigating molecular markers’ linkage with traits of agronomic importance. Breeders put a lot of emphasis on this type of markers, which are used in breeding programs implementation and speed up the process of a new variety development. In this paper, we construct a new, high-density linkage genetic map for Polish material on narrow-leafed lupin. The mapping population originated from crossing the Polish variety ‘Emir’ and the Belarusian breeding line ‘LAE-1’. A new map was constructed based on DArTseq markers—a new type of marker generated with the ne

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Downey, Suzanne L., and Amy F. Iezzoni. "Polymorphic DNA Markers in Black Cherry (Prunus serotina) Are Identified Using Sequences from Sweet Cherry, Peach, and Sour Cherry." Journal of the American Society for Horticultural Science 125, no. 1 (2000): 76–80. http://dx.doi.org/10.21273/jashs.125.1.76.

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Black cherry (Prunus serotina Ehrh.) is a common secondary forest species with a wide endemic distribution ranging from Nova Scotia south into Mexico, Ecuador, and Peru. Although planted in the United States for its valued lumber, black cherry is essentially a wild species with small fruit ≈6 to 10 mm in diameter. In contrast, in Mexico and Ecuador, domesticates of this species called Capulin, have much larger (2 to 2.5 cm in diameter) edible fruit. To date, no studies of the genetic diversity within North American black cherry or the ancestral origin of the Capulin types have been conducted.

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Soriano, Jose Miguel. "Molecular Marker Technology for Crop Improvement." Agronomy 10, no. 10 (2020): 1462. http://dx.doi.org/10.3390/agronomy10101462.

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Since the 1980s, agriculture and plant breeding have changed with the development of molecular marker technology. In recent decades, different types of molecular markers have been used for different purposes: mapping, marker-assisted selection, characterization of genetic resources, etc. These have produced effective genotyping, but the results have been costly and time-consuming, due to the small number of markers that could be tested simultaneously. Recent advances in molecular marker technologies such as the development of high-throughput genotyping platforms, genotyping by sequencing, and

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Moniruzzaman, M., R. Khatun, and AA Mintoo. "Application of marker assisted selection for livestock improvement in Bangladesh." Bangladesh Veterinarian 31, no. 1 (2015): 1–11. http://dx.doi.org/10.3329/bvet.v31i1.22837.

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Molecular markers usually do not have any biological effect. They are identifiable DNA sequences, found at specific locations of the genome, and transmitted from one generation to the next. Marker assisted selection (MAS) is a novel technique that can complement traditional breeding methods for rapid genetic gains. Genetic gain through selective breeding is the objective of a breeder to achieve long term improvement in animal and plant genomes; however the pace of improvement is inversely proportional to the Generation Interval. Genetic improvement in livestock, particularly those with long ge

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FUJII, HIROSHI, TATSUSHI OGATA, TAKEHIKO SHIMADA, et al. "MINIMAL MARKER: AN ALGORITHM AND COMPUTER PROGRAM FOR THE IDENTIFICATION OF MINIMAL SETS OF DISCRIMINATING DNA MARKERS FOR EFFICIENT VARIETY IDENTIFICATION." Journal of Bioinformatics and Computational Biology 11, no. 02 (2013): 1250022. http://dx.doi.org/10.1142/s0219720012500229.

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DNA markers are frequently used to analyze crop varieties, with the coded marker data summarized in a computer-generated table. Such summary tables often provide extraneous data about individual crop genotypes, needlessly complicating and prolonging DNA-based differentiation between crop varieties. At present, it is difficult to identify minimal marker sets — the smallest sets that can distinguish between all crop varieties listed in a marker-summary table — due to the absence of algorithms capable of such characterization. Here, we describe the development of just such an algorithm and Minima

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Nguyen, Lisa, Lucas-Sebastian Spitzhorn, and James Adjaye. "Constructing an Isogenic 3D Human Nephrogenic Progenitor Cell Model Composed of Endothelial, Mesenchymal, and SIX2-Positive Renal Progenitor Cells." Stem Cells International 2019 (May 2, 2019): 1–11. http://dx.doi.org/10.1155/2019/3298432.

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Urine has become the source of choice for noninvasive renal epithelial cells and renal stem cells which can be used for generating induced pluripotent stem cells. The aim of this study was to generate a 3D nephrogenic progenitor cell model composed of three distinct cell types—urine-derived SIX2-positive renal progenitor cells, iPSC-derived mesenchymal stem cells, and iPSC-derived endothelial cells originating from the same individual. Characterization of the generated mesenchymal stem cells revealed plastic adherent growth and a trilineage differentiation potential to adipocytes, chondrocytes

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CRÎNGAȘU (BĂRBIERU), ANCUȚA, MATILDA CIUCĂ, CRISTINA DANIEL, CĂLINA-PETRUȚA CORNEA, GHEORGHE ITTU, and ELENA-LAURA CONȚESCU. "Characterization of Checo/F95-927 pea (Pisum sativum L.) population for winter frost tolerance using molecular markers." Romanian Biotechnological Letters 26, no. 1 (2021): 2262–68. http://dx.doi.org/10.25083/rbl/26.1/2262.2268.

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Winter pea has several advantages on spring pea: higher yield, more rapid spring growth that helps this crop to compete weeds, wider growing area, etc. The aim of this paper was to evaluate the association of some molecular markers with frost tolerance of pea. Forty-one pea recombinant inbred lines (RILs), from F5 generation, obtained by crossing the winter cultivar Checo with the spring line F95-927 were examined during this work both by in vitro (molecular markers analysis) and in field experiments. The results obtained showed a significant correlation betweeen frost tolerance and molecular

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Özözen, Avni, M. Özgür Kayalica, and Gülgün Kayakutlu. "Day-ahead power market behavior for a small supplier: case of Turkish market." Environmental Economics 9, no. 2 (2018): 70–79. http://dx.doi.org/10.21511/ee.09(2).2018.05.

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The day-ahead power market has become more complex with the allowance of block purchases from private sales companies. Resource handling has become the prominent problem for both energy suppliers and energy distributers. Complexity of the problem forces the approach by each role player in the market. This research handles the market position of a small hydropower plant owner who has negligible effect on market price construction in a complex competition environment. Based on an optimum schedule of three days, this model proposes policies for the power generator to maximize its profits. An MILP

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Gao, Juan, Ian B. Denys, Luis Del Valle, Mihran V. Naljayan, and Daniel R. Kapusta. "2267 Radiofrequency renal denervation attenuates kidney fibrosis in spontaneously hypertensive rats." Journal of Clinical and Translational Science 2, S1 (2018): 25. http://dx.doi.org/10.1017/cts.2018.113.

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OBJECTIVES/SPECIFIC AIMS: The goal of this study was to investigate whether RF-RDN attenuates renal fibrosis and inflammation in SHR with established hypertension. METHODS/STUDY POPULATION: Twenty-two-week-old SHR received bilateral RF-RDN or Sham-RDN (Biosense Webster Stockert 70 generator and RF-probe). Four weeks later, SHR were sacrificed and paraffin sections of kidneys were stained for fibrosis by Masson’s trichrome staining. Kidney tissue were homogenized for measurement of cytokines levels by ELISA. RESULTS/ANTICIPATED RESULTS: The results showed that Sham-RDN treated SHR had extensive

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TANG, K. W., H. S. KWOK, WALLACE K. S. TANG, and K. F. MAN. "A CHAOS-BASED RANDOM NUMBER GENERATOR FOR EIGHT-BIT MICRO-CONTROLLER SYSTEM." International Journal of Bifurcation and Chaos 18, no. 03 (2008): 851–67. http://dx.doi.org/10.1142/s0218127408020719.

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Random number generators are widely used in different applications. However, it is difficult to obtain a good random number generator in low precision and resource constrained system, such as an eight-bit micro-controller system which is still commonly used in industrial and consumer markets. This paper provides a practical solution for this problem based on chaotic systems. By the use of a modified Chua's circuit, it is demonstrated that the sampled state, after post-processing by a high-dimensional chaotic map, can be used as a random source even in an eight-bit environment. The randomness o

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Gurumurthy, Channabasavaiah B., Poonam S. Joshi, Scott G. Kurz, et al. "Validation of Simple Sequence Length Polymorphism Regions of Commonly Used Mouse Strains for Marker Assisted Speed Congenics Screening." International Journal of Genomics 2015 (2015): 1–17. http://dx.doi.org/10.1155/2015/735845.

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Marker assisted speed congenics technique is commonly used to facilitate backcrossing of mouse strains in nearly half the time it normally takes otherwise. Traditionally, the technique is performed by analyzing PCR amplified regions of simple sequence length polymorphism (SSLP) markers between the recipient and donor strains: offspring with the highest number of markers showing the recipient genome across all chromosomes is chosen for the next generation. Although there are well-defined panels of SSLP makers established between certain pairs of mice strains, they are incomplete for most strain

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Wesołowski, Wojciech, Beata Domnicz, Joanna Augustynowicz, and Marek Szklarczyk. "VCF2CAPS–A high-throughput CAPS marker design from VCF files and its test-use on a genotyping-by-sequencing (GBS) dataset." PLOS Computational Biology 17, no. 5 (2021): e1008980. http://dx.doi.org/10.1371/journal.pcbi.1008980.

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Next-generation sequencing (NGS) is a powerful tool for massive detection of DNA sequence variants such as single nucleotide polymorphisms (SNPs), multi-nucleotide polymorphisms (MNPs) and insertions/deletions (indels). For routine screening of numerous samples, these variants are often converted into cleaved amplified polymorphic sequence (CAPS) markers which are based on the presence versus absence of restriction sites within PCR products. Current computational tools for SNP to CAPS conversion are limited and usually infeasible to use for large datasets as those generated with NGS. Moreover,

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Kafkas, Salih, Yıldız Doğan, Ali Sabır, Ali Turan, and Hasbi Seker. "Genetic Characterization of Hazelnut (Corylus avellana L.) Cultivars from Turkey Using Molecular Markers." HortScience 44, no. 6 (2009): 1557–61. http://dx.doi.org/10.21273/hortsci.44.6.1557.

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Genetic relationships among 18 Turkish hazelnut (Corylus avellana L.) cultivars were investigated using randomly amplified polymorphic DNA (RAPD), intersimple sequence repeat (ISSR), and amplified fragment length polymorphism (AFLP) markers. Twenty-five RAPD primers, 25 ISSR primers, and eight AFLP primer pairs generated a total of 434 polymorphic marker loci. The three marker systems were able to differentiate the cultivars. Genetic similarity index values ranged from a high of 0.96 for ‘Kan’ and ‘UzunMusa’ to a low of 0.73 for ‘Yassi Badem’ and ‘Kalinkara’. The genetic relationships were pre

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Lewers, Kim S., and Courtney A. Weber. "The Trouble with Genetic Mapping of Raspberry." HortScience 40, no. 4 (2005): 1108D—1108. http://dx.doi.org/10.21273/hortsci.40.4.1108d.

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Researchers developing new cultivars of red raspberry (Rubus idaeus subsp. idaeus L.) and black raspberry (R. occidentalis L.) observe progeny of breeding populations for several seasons to identify those that perform reliably. If a portion of any breeding population could be eliminated based on a qualitative character or molecular marker, resources used for that portion could be used for other progeny. Our objective is to identify such molecular markers for red raspberry and black raspberry. A black raspberry × red raspberry cross was made to develop a map of each parent, and an F2 population

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Ojha, BR. "Comparison of Eco-Mse and Pst-Mse Primer Combinations in Generating AFLP Map of Tomato." Journal of the Institute of Agriculture and Animal Science 26 (April 1, 2005): 27–35. http://dx.doi.org/10.3126/jiaas.v26i0.608.

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An experiment was conducted at Wageningen University and Research Centre (WUR), Unifarm, Haarweg, Wageningen, The Netherlands in 2000 to compare Eco-Mse and Pst-Mse primer combinations to generate AFLP markers of tomato. Back cross first (BC1) population ofLycopersicon esculentum cv money maker and Lycopersicon pennellii LA716 was used. Six primer combinations (three of Eco-Mse and three of Pst-Mse) were used. A total of 122 AFLP markers were found generating 76 markers by Eco-Mse primer combination and 46 markers by Pst-Mse primer combination. The average number of informative markers per pri

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Polashock, James J., and Nicholi Vorsa. "Development of SCAR Markers for DNA Fingerprinting and Germplasm Analysis of American Cranberry." Journal of the American Society for Horticultural Science 127, no. 4 (2002): 677–84. http://dx.doi.org/10.21273/jashs.127.4.677.

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DNA fingerprinting has been useful for genotypic classification of American cranberry (Vaccinium macrocarpon Ait.). Polymerase chain reaction (PCR) based methodologies including randomly amplified polymorphic DNA (RAPD) markers are relatively easy to use, and inexpensive as compared to other methods. However, RAPD markers have some limitations including seamless interlaboratory transferability and susceptibility to certain types of error. An alternative method, sequence characterized amplified regions (SCARs), was developed for cranberry germplasm analysis. Nine primer sets were designed from

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Young, Roberto A., and James D. Kelly. "RAPD Markers Flanking the Are Gene for Anthracnose Resistance in Common Bean." Journal of the American Society for Horticultural Science 121, no. 1 (1996): 37–41. http://dx.doi.org/10.21273/jashs.121.1.37.

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Incorporation of the dominant gene Are, of Middle American origin, into commercial cultivars of Phaseolus vulgaris L., has been the main disease control strategy of plant breeders to limit the potential damage of Colletotrichum lindemuthianum (Sacc. & Magnus.) Lams.-Scrib. A random amplified polymorphic DNA (RAPD) marker designated OQ41440, generated by a 5′-AGTGCGCTGA-3′ decamer primer, was found tightly linked in coupling with the Are gene. OQ41440 mapped at 2.0 ± 1.4 centimorgans (cM) from the Are allele in the Andean genetic background and at 5.5 ± 2.3 CM in the Middle American backgro

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Hawkins, Leigh K., Fenny Dane, Thomas L. Kubisiak, Billy B. Rhodes, and Robert L. Jarret. "Linkage Mapping in a Watermelon Population Segregating for Fusarium Wilt Resistance." Journal of the American Society for Horticultural Science 126, no. 3 (2001): 344–50. http://dx.doi.org/10.21273/jashs.126.3.344.

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Isozyme, randomly amplified polymorphic DNA (RAPD), and simple sequence repeats (SSR) markers were used to generate a linkage map in an F2 and F3 watermelon [Citrullus lanatus (Thumb.) Matsum. & Nakai] population derived from a cross between the fusarium wilt (Fusarium oxysporum f. sp. niveum) susceptible `New Hampshire Midget' and resistant PI 296341-FR. A 112.9 cM RAPD-based map consisting of 26 markers spanning two linkage groups was generated with F2 data. With F3 data, a 139 cM RAPD-based map consisting of 13 markers covering five linkage groups was constructed. Isozyme and SSR marker

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Fang, Ying, Jane Christopher-Hennings, Elizabeth Brown, et al. "Development of genetic markers in the non-structural protein 2 region of a US type 1 porcine reproductive and respiratory syndrome virus: implications for future recombinant marker vaccine development." Journal of General Virology 89, no. 12 (2008): 3086–96. http://dx.doi.org/10.1099/vir.0.2008/003426-0.

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Porcine reproductive and respiratory syndrome virus (PRRSV) continues to be a major problem in the pork industry worldwide. The limitations of current PRRSV vaccines require the development of a new generation of vaccines. One of the key steps in future vaccine development is to include markers for diagnostic differentiation of vaccinated animals from those naturally infected with wild-type virus. Using a cDNA infectious clone of type 1 PRRSV, this study constructed a recombinant green fluorescent protein (GFP)-tagged PRRSV containing a deletion of an immunogenic epitope, ES4, in the nsp2 regi

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SENAN, Siju, Dhanya KIZHAKAYIL, Bhaskaran SASIKUMAR, and Thotten Elampilay SHEEJA. "Methods for Development of Microsatellite Markers: An Overview." Notulae Scientia Biologicae 6, no. 1 (2014): 1–13. http://dx.doi.org/10.15835/nsb619199.

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Microsatellite or Simple Sequence Repeat (SSR) markers have evolved to the status of a most versatile and popular genetic marker in a ubiquity of plant systems. Due to their co-dominant, hyper-variable and multiallelic nature, they are the prominent markers of choice for fingerprinting, conservation genetics, plant breeding and phylogenetic studies. Despite its development of a new set of SSR markers for a species remained time consuming and expensive for many years. However, with the recent advancement in genomics, new strategies/protocols are now available for the generation of SSR markers.

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Meuwissen, T. H. E., B. J. Hayes, and M. E. Goddard. "Prediction of Total Genetic Value Using Genome-Wide Dense Marker Maps." Genetics 157, no. 4 (2001): 1819–29. http://dx.doi.org/10.1093/genetics/157.4.1819.

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Abstract Recent advances in molecular genetic techniques will make dense marker maps available and genotyping many individuals for these markers feasible. Here we attempted to estimate the effects of ∼50,000 marker haplotypes simultaneously from a limited number of phenotypic records. A genome of 1000 cM was simulated with a marker spacing of 1 cM. The markers surrounding every 1-cM region were combined into marker haplotypes. Due to finite population size (Ne = 100), the marker haplotypes were in linkage disequilibrium with the QTL located between the markers. Using least squares, all haploty

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