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Dissertations / Theses on the topic 'Genes gyrA e gyrB'

Author: Grafiati
Published: 4 June 2021
Last updated: 6 February 2022

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1

Moura, Vinicius Calado Nogueira de. "Caracterização da resistência a quinolonas em Mycobacterium abscessus subsp. bolletii e outras micobactérias de crescimento rápido relacionadas." Universidade do Estado do Rio de Janeiro, 2011. http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=6113.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>Em diversos estados do Brasil, foram relatadas epidemias de infecções causadas por micobactérias de crescimento rápido (MCR) desde o ano 2000. A maioria dos casos foi principalmente associada ao clone BRA100 de Mycobacterium massiliense, recentemente renomeada para Mycobacterium abscessus subsp. bolletii, isolado de pacientes submetidos a procedimentos invasivos nos quais os instrumentos médicos não foram adequadamente esterilizados e/ou desinfetados. Sendo as quinolonas uma opção no tratamento de infecções por MCR e sugerida para
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2

Batista, Maria de Lurdes. "Estudo de uma mutação no gene gyrB de Pseudomonas aeruginosa." Master's thesis, Universidade de Aveiro, 2008. http://hdl.handle.net/10773/869.

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Mestrado em Biologia Molecular e Celular<br>Com o intuito de aprendizagem e aplicação de algumas técnicas de biologia molecular que possam representar uma mais valia no desempenho de funções no ambiente hospitalar, o trabalho prático realizado foi adaptado e enquadrado no contexto de um estudo já iniciado. Num trabalho anterior (Ferreira S. 2005), foi encontrada uma nova mutação no codão 464 do gene gyrB, que conduzia à substituição de um amino ácido serina por uma tirosina numa estirpe hospitalar de Pseudomonas aeruginosa (Pa30) resistente às quinolonas. P. aeruginosa é um importante patogéni
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3

SCAVUZZI, Alexsandra Maria Lima. "lnvestigação de genes de resistência a quinolonas e avaliação de alterações ultraestruturais com o uso de antimicrobianos em isolados de Klebsiella pneumoniae portadores do gene blaKPC." reponame:Repositório Institucional da UFPE, 2015. https://repositorio.ufpe.br/handle/123456789/14078.

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Submitted by Fabio Sobreira Campos da Costa (fabio.sobreira@ufpe.br) on 2015-05-26T17:18:09Z No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Tese doutorado Alexsandra Scavuzzi.pdf: 8878953 bytes, checksum: 50dba197129c93a6d62fed598bc1f2d1 (MD5)<br>Made available in DSpace on 2015-05-26T17:18:09Z (GMT). No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Tese doutorado Alexsandra Scavuzzi.pdf: 8878953 bytes, checksum: 50dba197129c93a6d62fed598bc1f2d1 (MD5) Previous issue date: 2015-02-27<br>Foi inv
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4

Hogan, Tiffany Rose School of Medical Science UNSW. "Molecular changes in the topoisomerase genes, gyrA and parC, and their contribution to fluoroquinolone resistance in the pathogenic Neisseria." Awarded by:University of New South Wales. School of Medical Science, 2006. http://handle.unsw.edu.au/1959.4/27298.

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This thesis examined molecular changes in the quinolone-resistance determining regions (QRDRs) of the topoisomerase genes, gyrA and parC of Neisseria gonorrhoeae and Neisseria meningitidis and their contribution to fluoroquinolone resistance (FQR). Initially models of FQR emergence were developed from analysis of resistant mutants generated in vitro. The effects of the nature and order of sequential changes in GyrA and ParC on FQR were explored by correlating QRDR changes with ciprofloxacin minimum inhibitory concentration (MIC) determinations. The in vitro models were validated by comparisons
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5

Al-hashimi, Sora. "Kvantifiering med digital droplet polymerase chain reaction av gyrA-genen med och utan mutationen S83L." Thesis, Örebro universitet, Institutionen för hälsovetenskaper, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-92989.

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Den vanligaste cancerformen hos män är prostatacancer, med 10 000 nya dödsfall årligen. Vid prostatacancerdiagnostik utförs prostata biopsi. För att minska risken för komplikationer i samband med biopsi ges i Sverige en singeldos av antibiotikapreparatet Ciprofloxacin. Andelen bakterier som är resistenta mot ciprofloxacin har ökat. För detektion av genmutationer som orsakar antibiotikaresistens kan droplet digital PCR (ddPCR) användas. Det är en metod som ger en absolut kvantifiering av antalet DNA-sekvenser. Den är baserad på vatten olja-emulsionsdroppsystem.  Syftet med denna studie var att
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6

MUNDELL, J. NICOLE. "PHYLOGENETIC ANALYSIS OF KENTUCKY STRAINS OF XYLELLA FASTIDIOSA." UKnowledge, 2005. http://uknowledge.uky.edu/gradschool_theses/406.

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Phytopathogenic bacterium, Xylella fastidiosa, causes a number of economically important diseases, including Pierces disease (PD) of grape and bacterial leaf scorch (BLS) of a number of landscape trees. In Kentucky (KY), BLS affects a number of shade trees including many oak and maple species. In 2001, PD was diagnosed in grapevines in western KY. Xylella fastidiosa is also detected in many asymptomatic landscape plants and grasses. It was the goal of this research to identify hosts of X. fastidiosa around KY and use phylogenetic analysis to compare sequences of the 16S rDNA and gyrase B (gyrB
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7

Zhu, Liwei. "Identification of secondary metabolite gene clusters of bacteria from south pacific gyre subseafloor sediment." Thesis, University of Rhode Island, 2014. http://pqdtopen.proquest.com/#viewpdf?dispub=1555704.

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<p> Secondary metabolites are organic compounds that are not directly involved in the key processes (growth, reproduction and development) of an organism. They are commonly targeted in pharmaceutical science for drug discovery. Secondary metabolites that have been used in drug discovery have been derived from plants, invertebrates and microbes. Microbes, bacteria in particular, have contributed greatly and will continue to play an important role in new drug discovery. Among the bacteria from all environments, marine bacteria are a vast reservoir for many potential useful bioactive compounds. R
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8

Ribeiro, Aldemir Reginato. "Salmonella enteritidis de origem aviária: determinação de padrões de resistência antimicrobiana, detecção de mutação no gene gyrA de cepas resistentes ao ácido nalidíxico, fagotipagem e ribotipagem." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2007. http://hdl.handle.net/10183/15611.

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Este trabalho foi conduzido com o objetivo de gerar dados de resistência a agentes antimicrobianos de Salmonella Enteritidis (SE) isoladas de amostras clínicas e do ambiente criatório de aves, nos anos de 1999, 2000 e 2001, de cortes de frango, no ano de 1996, ambos na região Sul, bem como de carcaças de frango, nos anos de 2004 e 2005, na região Nordeste, detectar mutação no gene gyrA da região determinante de resistência a quinolonas das cepas que apresentaram resistência ao ácido nalidíxico e fagotipá-las. Realizou-se também a ribotipagem de 28 cepas de SE isoladas de carcaças resfriadas de
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9

Reibel, Florence. "Mycobacterium leprae : de l’épidémiologie à la caractérisation de l’ADN gyrase." Electronic Thesis or Diss., Sorbonne université, 2018. http://www.theses.fr/2018SORUS560.

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Deux cent mille cas de lèpre surviennent chaque année dans le monde, surtout dans les pays en voie de développement. Le laboratoire d’accueil ayant une double activité de surveillance de la lèpre et de recherche centrée sur l’étude des mécanismes de résistance aux antituberculeux, les objectifs du travail s’inscrivent dans des enjeux médicaux et fondamentaux. Un 1er travail de génotypage de M. leprae nous a permis de montrer le caractère acquis dans des territoires hors France métropolitaine des cas de lèpre diagnostiqués sur le territoire national. L’équipement enzymatique de M. leprae est re
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10

Anampa, Álvarez Diego Enrique. "Detección de genes Gyr(A) y Erm(B) de resistencia antimicrobiana en cepas patógenas de Campylobacter spp. aisladas de canales de pollos comercializados en Lima Metropolitana." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2020. https://hdl.handle.net/20.500.12672/15950.

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El presente estudio tiene como objetivo la detección de genes de resistencia a antibióticos gyrA y ermB en cepas de Campylobacter spp. aisladas de piel de pollo comercializadas en los Mercado Metropolitano de Lima. Para proceder se tomaron muestras de 120 pieles de pollo de los mercados municipales de Lima Metropolitana (Santa Anita (20), San Martín de Porres (30) e Independencia (70)). Las muestras se sometieron a un proceso de enriquecimiento previo de 24 horas en caldo Preston a 42 ° C en un ambiente microaerófilo. Una submuestra de 100 µl se incubó en agar mCCDA durante 48 h en las
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11

Chuang, Jiing-Guang, and 莊景光. "Cloning of gyrB and gyrA Genes of Phytoplasma Associated with Peanut Witches'' Broom." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/85713838251226307105.

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碩士<br>國立臺灣大學<br>植物病理學研究所<br>88<br>DNA gyrase is a type II topoisomerase that is a tetrameric molecule composed of two A and two B subunits, which are encoded by the gyrA and gyrB genes, respectively. In order to clone and analyses the gyrB gene, a pair of oligo-nucleotides PCR primer GBF2/ GBR3 was designed according to the sequences of the gyrB gene of Acholeplasma laidlawii, Escherichia coli, Bacillus sp., Staphylococcus aureus, Streptococcus pneumoniae, Streptomyces spheroides, Spiroplasma citri and Mycoplasma spp. Total DNA from diseased periwinkle infected with phytoplasma associated wi
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12

Wang, Yung-Ting, and 王筠婷. "Molecular mechanism of Ciprofloxacin resistance in Pseudomonas aeruginosa isolates associated with target gene of gyrA, gyrB, parC and parE gene." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/65450415560454629208.

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碩士<br>高雄醫學大學<br>醫學檢驗生物技術學研究所<br>100<br>Pseudomonas aeruginosa is an important pathogen in nosocomial infections and often shows multiple antibiotics resistance. Therapy for P. aeruginosa infections is currently limited to the use of a few antibiotics, including fluoroquinolones. In this study, ciprofloxacin (CIP) is one of fluoroquinolones class antibiotics used for elucidating molecular mechanism of resistance in P. aeruginosa isolates. Resistant to ciprofloxacin is associated with target gene mutations (such as gyrA, gyrB, parC and parE genes), and/or overexpression efflux pump systems. The
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13

Rigouts, L., N. Coeck, M. Gumusboga, et al. "Specific gyrA gene mutations predict poor treatment outcome in MDR-TB." 2015. http://hdl.handle.net/10454/17328.

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Yes<br>Mutations in the gyrase genes cause fluoroquinolone resistance in Mycobacterium tuberculosis. However, the predictive value of these markers for clinical outcomes in patients with MDR-TB is unknown to date. The objective of this study was to determine molecular markers and breakpoints predicting second-line treatment outcomes in M. tuberculosis patients treated with fourth-generation fluoroquinolones. We analysed treatment outcome data in relation to the gyrA and gyrB sequences and MICs of ofloxacin, gatifloxacin and moxifloxacin for pretreatment M. tuberculosis isolates from 181 MDR
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14

Kuo, Ching-Hui, and 郭靜蕙. "Quinolone Resistant Mutations of gyrA Gene of Ornithobacterium rhinotracheale in Poultry in Taiwan." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/85677154707718027865.

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碩士<br>臺灣大學<br>獸醫學研究所<br>98<br>A total of 86 isolates of Ornithobacterium rhinotracheale were obtained from veterinary preventive medicine of National Taiwan University. Species include chickens, turkeys, pigeons, ostrichs, quails, and Accipiter trivirgatus respectively from 2004 to 2006. This study purpose is to analyze the quinolone resistant mutations of gyrA gene of O. rhinotracheale in Taiwan. Sixty-two (72.1 %, 62/86) quinolone resistant O. rhinotracheale strains were found by agar dilution method with the antibiotics nalidixic acid, norfloxacin, ofloxacin, enrofloxacin and ciprofloxacin
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15

Alves, Ana Rita Martins. "Contribuição de mutações genéticas no gene gyrA para a resistência a fluoroquinolonas por Arcobacter butzleri." Master's thesis, 2018. http://hdl.handle.net/10400.6/9993.

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Arcobacter butzleri é considerado o quarto agente de Campylobacter-like organism isolado a partir de amostras diarreicas em humanos. As fluoroquinolonas são normalmente utilizadas na primeira linha de tratamento de infeções severas causadas por Arcobacter spp.. A elevada frequência de resistência a esta classe de antibióticos observada em A. butzleri poderá constituir uma grande preocupação para a saúde pública. Assim, este trabalho tem como objetivo avaliar os mecanismos de resistência às fluoroquinolonas em A. butzleri, tendo como foco a contribuição de mutações espontâneas, bem como na sua
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16

Shi, Yanmei. "Measurement of (in situ) expression of proteorhodopsin genes at the North Pacific Central Gyre Station Aloha." 2005. http://hdl.handle.net/1903/2982.

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Thesis (M.S.) -- University of Maryland, College Park, 2005.<br>Thesis research directed by: Marine, Estuarine, Environmental Sciences Graduate Program. Title from t.p. of PDF. Includes bibliographical references. Published by UMI Dissertation Services, Ann Arbor, Mich. Also available in paper.
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