Academic literature on the topic 'Genetic engineering Recombinant DNA'
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Journal articles on the topic "Genetic engineering Recombinant DNA"
Blank, Robert H. "Politics and Genetic Engineering." Politics and the Life Sciences 11, no. 1 (February 1992): 81–85. http://dx.doi.org/10.1017/s0730938400017226.
Full textDodds, John H., and Jesse M. Jaynes. "Crop Plant Genetic Engineering: Science Fiction to Science Fact." Outlook on Agriculture 16, no. 3 (September 1987): 111–15. http://dx.doi.org/10.1177/003072708701600303.
Full textBogomolova, E. G., P. M. Kopeykin, and A. A. Tagaev. "Genetic engineering approaches to the development of modern therapeutics." Medical academic journal 20, no. 3 (September 15, 2020): 49–60. http://dx.doi.org/10.17816/maj34092.
Full textLjubijankic, Goran. "Genetic engineering: from a clone to a protein." Genetika 34, no. 2-3 (2002): 73–84. http://dx.doi.org/10.2298/gensr0203073l.
Full textDuster, Troy. "Cline's Recombinant DNA Experiment as Political Rashomon." Politics and the Life Sciences 6, no. 1 (August 1987): 15–18. http://dx.doi.org/10.1017/s0730938400002677.
Full textZOLLER, M. "New recombinant DNA methodology for protein engineering." Current Biology 2, no. 9 (September 1992): 510. http://dx.doi.org/10.1016/0960-9822(92)90692-4.
Full textMUYSSON, DAVID J., and ANN M. VERRINDER GIBBINS. "THE ALTERATION OF MILK CONTENT BY GENETIC ENGINEERING AND RECOMBINANT DNA-MEDIATED SELECTION TECHNIQUES." Canadian Journal of Animal Science 69, no. 3 (September 1, 1989): 517–27. http://dx.doi.org/10.4141/cjas89-064.
Full textBiagetti, Marco, Francesca Vitellozzi, and Carla Ceoloni. "Physical mapping of wheat-Aegilops longissima breakpoints in mildew-resistant recombinant lines using FISH with highly repeated and low-copy DNA probes." Genome 42, no. 5 (October 1, 1999): 1013–19. http://dx.doi.org/10.1139/g98-172.
Full textBernstein, R. L., and Sheldon Krimsky. "Genetic Alchemy, the Social History of the Recombinant DNA Controversy." Leonardo 18, no. 2 (1985): 121. http://dx.doi.org/10.2307/1577892.
Full textTirrell, David A., Maurille J. Fournier, and Thomas L. Mason. "Genetic Engineering of Polymeric Materials." MRS Bulletin 16, no. 7 (July 1991): 23–28. http://dx.doi.org/10.1557/s0883769400056505.
Full textDissertations / Theses on the topic "Genetic engineering Recombinant DNA"
黃雅誼 and Nga-yi Queenie Wong. "DNA engineering utilizing thymidylate synthase A (thyA) selection system in Escherichia coli." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B31226851.
Full textWong, Nga-yi Queenie. "DNA engineering utilizing thymidylate synthase A (thyA) selection system in Escherichia coli /." Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?
Full textDaum, Marilyn. "Geneplanner : a prototype of an expert system to assist with chemical DNA gene synthesis planning /." Online version of thesis, 1989. http://hdl.handle.net/1850/10576.
Full textKepple, Kevin V. "Analysis of the binding mechanisms and cellular targets of peptide inhibitors that block site-specific recombination in vitro /." Diss., Connect to a 24 p. preview or request complete full text in PDF formate. Access restricted to UC campuses, 2006. http://wwwlib.umi.com/cr/ucsd/fullcit?p3208620.
Full textComeaux, Jay Louis. "Transfer of plasmids by genetically-engineered Erwinia carotovora." Thesis, Virginia Tech, 1989. http://hdl.handle.net/10919/45933.
Full textThe ability of a genetically-engineered Erwirzia carotovora subsp. carotovora (Ecc) strain to transfer recombinant chromosomal DNA or plasmids to wildtype Ecc or Pseudomonas fluorescens was tested on filters, within soil microcosms, and in planta. Ecc was engineered by chromosomal insertion of a disarmed endo-pectate lyase gene marked with a 1.4kb DNA fragment conferring kanamycin resistance. Plasmids RPI and pBR322 were introduced separately into engineered Ecc clones. These strains served as donors in genetic transfer experiments. No transfer of the inserted kan marker or of pBR322 was observed under any experimental condition. In filter matings, RPI was transferred to wildtype Ecc at a frequency of 3.6 X 10-2 transconjugants per donor (TPD) and to P. fluorescens at a frequency of 2.4 X 10-5 TPD. In matings conducted in potato tubers inoculated using sewing needles, the respective frequencies were 4.0 X 10-4 and 2.0 X 10-4, while matings on potato slices yielded frequencies of 4.7 X 10-2 and 2.3 X 10-2. In soil microcosms, the maximum transfer frequencies observed were 2.3 X 10-3 and 8.4 X 10-5 TPD.
Master of Science
Dambros, Régia Maria Feltrin. "Amplificação, clonagem e expressão de proteína recombinante do vírus da doença de Aujeszky em sistema de baculovírus para utilização em programa de controle e erradicação." Universidade do Estado de Santa Catarina, 2006. http://tede.udesc.br/handle/handle/966.
Full textCoordenação de Aperfeiçoamento de Pessoal de Nível Superior
Aujeszky s disease (AD) is an infect-contagious illness that causes serious economical damages to the producer and the swine industry. Aiming to develop mechanisms and to improve technologies that are faster, more sensitive and more specific for diagnosis and for use in free areas or in AD s eradication programs, the sequence codifier of the glycoprotein E (gE) of Aujeszky s disease virus (ADV) was amplified, cloned and expressed. Through genetic engineering the sequence of the gE gene was propagated in an host organism. The gE was amplified by the technique of polimerase chain reaction (PCR), cloned in the vector pGem®-T Easy and transformed in competent cells of Escherichia coli, DH-5a . The clone obtained was sub-cloned in the expression plasmid pFastBac 1, which contains the promoter gene of the polyhedrin. The obtained subclone was analyzed inside for certification of its correct plasmid orientation with the restriction endonucleases BamH I and EcoR I. The sub-clone with the correct orientation had its DNA extracted and used for transposition inside the bacmid (recombinant baculovirus and helper plasmid with competent DH10Bac cell). Colonies with inserted gE were selected by the phenotype of the colony, which expresses white color when cloned. White recombinant colonies had their DNA extracted and used for cotransfection in insect cells Trichoplusia ni (BTI-Tn5B1-4). The recombinant-gE baculovirus was inoculated in cultured cells and expressed the recombinant gE by PCR and Western blotting . The recombinant-gE baculovirus containing only the gE gene of the VDA will be used for antigen and monoclonal antibodies production, which will aid in the development of a more sensitive, specific and safer for the use in VDA free regions
A doença de Aujeszky (DA) é uma enfermidade infecto-contagiosa que causa graves prejuízos econômicos ao produtor e à agroindústria suinícola. Com o objetivo de desenvolver insumos e aprimorar tecnologias que sejam mais rápidas, sensíveis e específicas de diagnóstico para uso em regiões livres ou em erradicação da DA, a seqüência codificadora da glicoproteína E (gE) do vírus da doença de Aujeszky (VDA) foi amplificada, clonada e expressada. Através da engenharia genética a seqüência do gene da gE foi propagada em um organismo hospedeiro. A gE foi amplificada pela técnica da reação em cadeia da polimerase (PCR) , clonada no vetor pGem®-T Easy e transformada em células competentes de Escherichia coli, DH-5a . O clone obtido foi subclonado no plasmídeo de expressão pFastBac 1, o qual possui o sítio promotor do gene da poliedrina. O subclone obtido foi analisado para certificação de sua orientação correta dentro do plasmídeo com as endonucleases de restrição BamH I e EcoR I. O subclone com a orientação correta teve seu DNA extraído e usado para a transposição dentro do bacmid (baculovírus recombinante e plasmídeo helper em célula competente DH10Bac ). As colônias com inserto gE foram selecionadas pelo fenótipo da colônia, a qual expressa cor branca quando clonada. As colônias brancas recombinantes tiveram seu DNA extraído e usado para a co-transfecção em células do inseto Trichoplusia ni (BTITn5B1- 4). O baculovírus gE-recombinante ao ser inoculado em cultivo celular, expressou a gE recombinante, comprovada pela técnica de PCR e Western blotting . O baculovírus gE-recombinante contendo apenas o gene da gE do VDA será utilizado para produção de antígeno e de anticorpos monoclonais, o que auxiliará no desenvolvimento de um teste de diagnóstico mais sensível, específico e mais seguro para uso em áreas livres do VDA
MURATA, YOKO. "Sintese e caracterizacao do hormonio tireotrofico humano recombinante (rec-hTSH) contendo uma sub unidade beta quimerica (rec-hTSHbeta-CTEP hCGbeta)." reponame:Repositório Institucional do IPEN, 1995. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10432.
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Tese (Doutoramento)
IPEN/T
Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP
MATHOR, MONICA B. "Estudos da expressao genica mediante utilizacao de queratinocitos humanos normais transduzidos com o gene do hormonio de crescimento humano .Possivel utilizacao em terapia genica." reponame:Repositório Institucional do IPEN, 1994. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10410.
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Tese (Doutoramento)
IPEN/T
Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP
Cousseau, Philippe. "Traitement d'image et traitement graphique en genie genetique : application a l'analyse d'images autoradiographiques et a la representation de cartes de molecules d'adn recombinant." Université Louis Pasteur (Strasbourg) (1971-2008), 1986. http://www.theses.fr/1986STR13037.
Full textJones, Mary Ellen. "Politically Corrected Science: The Early Negotiation of U.S. Agricultural Biotechnology Policy." Diss., Virginia Tech, 1999. http://hdl.handle.net/10919/29868.
Full textPh. D.
Books on the topic "Genetic engineering Recombinant DNA"
Adrianne, Massey, ed. Recombinant DNA and biotechnology. Washington, D.C: ASM Press, 1996.
Find full textB, Primrose S., ed. Principles of gene manipulation: An introduction to genetic engineering. 3rd ed. Oxford: Blackwell Scientific Publications, 1985.
Find full textB, Primrose S., ed. Principles of gene manipulation: An introduction to genetic engineering. 5th ed. Oxford: Blackwell Scientific, 1994.
Find full textB, Primrose S., ed. Principles of gene manipulation: An introduction to genetic engineering. 4th ed. Oxford [England]: Blackwell Scientific Publications, 1989.
Find full textM, Twyman Richard, and Old R. W, eds. Principles of gene manipulation. 6th ed. Oxford: Blackwell Scientific, 2001.
Find full textJ, Pasternak Jack, and Patten Cheryl L, eds. Molecular biotechnology: Principles and applications of recombinant DNA. 4th ed. Washington, DC: ASM Press, 2010.
Find full textRecombinant DNA basics: A primer : programmed learning. Denver, CO: Berkeley Scientific Publications, 1993.
Find full textKreuzer, Helen. Recombinant DNA and biotechnology: A guide for students. 2nd ed. Washington, D.C: ASM Press, 2001.
Find full textAdrianne, Massey, ed. Recombinant DNA and biotechnology: A guide for teachers. 2nd ed. Washington, D.C: ASM Press, 2001.
Find full textBook chapters on the topic "Genetic engineering Recombinant DNA"
Young, Richard A., and Ronald W. Davis. "Immunoscreening λgtll Recombinant DNA Expression Libraries." In Genetic Engineering: Principles and Methods, 29–41. Boston, MA: Springer US, 1985. http://dx.doi.org/10.1007/978-1-4684-4973-0_2.
Full textLuciw, Paul, Debbie Parkes, Gary Van Nest, Dino Dina, Kathleen Hendrix, and Murray B. Gardner. "Recombinant DNA Approaches to Feline Leukemia Virus Immunization." In Genetic Engineering of Animals, 207–15. Boston, MA: Springer US, 1986. http://dx.doi.org/10.1007/978-1-4684-5110-8_18.
Full textAkram, Muhammad, Farhat Jabeen, Muhammad Daniyal, Rida Zainab, Usman ul Haq, Charles Oluwaseun Adetunji, Chukwuebuka Egbuna, Benson C. Ephraim-Emmanuel, Kingsley C. Patrick-Iwuanyanwu, and Ahiakwo B. Ogbo. "Genetic Engineering of Novel Products of Health Significance: Recombinant DNA Technology." In Functional Foods and Nutraceuticals, 595–611. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-42319-3_26.
Full textSimpson, J., and L. Herrera-Estrella. "DNA Recombinants and Transformation of Agricultural Crops." In Plant Protoplasts and Genetic Engineering II, 75–98. Berlin, Heidelberg: Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-74454-9_4.
Full textSederoff, Ronald, Anne-Marie Stomp, Babette Gwynn, Elizabeth Ford, Carol Loopstra, Paul Hodgskiss, and W. Scott Chilton. "Application of Recombinant DNA Techniques to Pines: A Molecular Approach To Genetic Engineering in Forestry." In Cell and Tissue Culture in Forestry, 314–29. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-017-0994-1_19.
Full textGrompe, Marcus, Wade Johnson, and J. Larry Jameson. "Recombinant DNA and Genetic Techniques." In Principles of Molecular Medicine, 9–24. Totowa, NJ: Humana Press, 1998. http://dx.doi.org/10.1007/978-1-59259-726-0_2.
Full textRazin, Aharon. "DNA Methylases." In Genetic Engineering, 1–11. Boston, MA: Springer US, 1989. http://dx.doi.org/10.1007/978-1-4615-7084-4_1.
Full textBlanchard, Alan. "Synthetic DNA Arrays." In Genetic Engineering, 111–23. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4899-1739-3_5.
Full textPriyadarshini, Anjali, and Prerna Pandey. "Genetic Manipulation by Recombinant DNA Technology." In Molecular Biology, 219–74. Toronto ; New Jersey : Apple Academic Press, 2018.: Apple Academic Press, 2018. http://dx.doi.org/10.1201/b22354-5.
Full textHochuli, Erich. "Purification of Recombinant Proteins with Metal Chelate Adsorbent." In Genetic Engineering, 87–98. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4613-0641-2_6.
Full textConference papers on the topic "Genetic engineering Recombinant DNA"
Junid, Syed Abdul Mutalib Al, Muhd Shahirad Reffin, Zulkifi Abd Majid, Nooritawati Md Tahir, and Muhammad Adib Haron. "Implementation of genetic algorithm for optimizing DNA sequence alignment." In 2012 IEEE Business Engineering and Industrial Applications Colloquium (BEIAC). IEEE, 2012. http://dx.doi.org/10.1109/beiac.2012.6226111.
Full textHussien, Hany H. "DNA Computing for RGB image Encryption with Genetic Algorithm." In 2019 14th International Conference on Computer Engineering and Systems (ICCES). IEEE, 2019. http://dx.doi.org/10.1109/icces48960.2019.9068136.
Full textMuttakin, Al, and Mohammad Rezwanul Huq. "Motif discovery in unaligned DNA sequences using genetic algorithm." In 2017 4th International Conference on Advances in Electrical Engineering (ICAEE ). IEEE, 2017. http://dx.doi.org/10.1109/icaee.2017.8255450.
Full textPeng, Xinmei, Xuedong Zheng, Bin Wang, Changjun Zhou, and Qiang Zhang. "A micro-genetic algorithm for DNA encoding sequences design." In 2016 2nd International Conference on Control Science and Systems Engineering (ICCSSE). IEEE, 2016. http://dx.doi.org/10.1109/ccsse.2016.7784342.
Full textLiu, Xiaohong. "Notice of Retraction: A Report on the Teaching Contents and Methods of DNA Recombinant Technology in the Course Molecular Biology." In 2011 5th International Conference on Bioinformatics and Biomedical Engineering. IEEE, 2011. http://dx.doi.org/10.1109/icbbe.2011.5780096.
Full textTremberger, George, S. Dehipawala, A. Nguyen, E. Cheung, R. Sullivan, T. Holden, D. Lieberman, and T. Cheung. "Complexity of genetic sequences modified by horizontal gene transfer and degraded-DNA uptake." In SPIE Optical Engineering + Applications, edited by Richard B. Hoover, Gilbert V. Levin, Alexei Yu Rozanov, and Nalin C. Wickramasinghe. SPIE, 2015. http://dx.doi.org/10.1117/12.2186550.
Full textChen Xiao and Zhang Ridong. "A DNA genetic algorithm with reconstruct operator for chemical process modeling." In 2011 Second International Conference on Mechanic Automation and Control Engineering (MACE). IEEE, 2011. http://dx.doi.org/10.1109/mace.2011.5987087.
Full textGuo, Y. C., H. Wang, and B. L. Zhang. "Blind equalization algorithms based on DNA genetic optimization of artificial fish swarm." In International Conference on Automation, Mechanical and Electrical Engineering. Southampton, UK: WIT Press, 2014. http://dx.doi.org/10.2495/amee140922.
Full textQiu, Weiguo, Arjun Stokes, Joseph Cappello, and Xiaoyi Wu. "Electrospinning of Recombinant Protein Polymer Nanofibers." In ASME 2009 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2009. http://dx.doi.org/10.1115/sbc2009-206352.
Full textWu, Huapeng, and Yecai Guo. "DNA Genetic Optimization Bat Algorithm Based Fractionally Spaced Multi-modulus Blind Equalization Algorithm." In 2015 International Industrial Informatics and Computer Engineering Conference. Paris, France: Atlantis Press, 2015. http://dx.doi.org/10.2991/iiicec-15.2015.133.
Full textReports on the topic "Genetic engineering Recombinant DNA"
Beachy, R. N. [Genetic engineering with a gene encoding a soybean storage protein to identify DNA sequences to control its expression]: Annual report, 1993. Office of Scientific and Technical Information (OSTI), December 1993. http://dx.doi.org/10.2172/10133802.
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