Academic literature on the topic 'Genetic engineering Recombinant DNA'

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Journal articles on the topic "Genetic engineering Recombinant DNA"

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Blank, Robert H. "Politics and Genetic Engineering." Politics and the Life Sciences 11, no. 1 (February 1992): 81–85. http://dx.doi.org/10.1017/s0730938400017226.

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THE PURPOSE OF THIS short note is to update readers on recent developments in, and political implications of, genetic engineering, a critical tool in the expanding field of biotechnology. Based on new understanding of the mechanisms of DNA, molecular biologists are now able to chemically cut genes or sets of genes from one organism and splice them into the DNA of another. This is called recombinant DNA (rDNA) technology. Although these techniques were first applied to bacteria and yeasts, in the last decade researchers have made remarkable strides in putting foreign genes into more complex plants and animals.
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Dodds, John H., and Jesse M. Jaynes. "Crop Plant Genetic Engineering: Science Fiction to Science Fact." Outlook on Agriculture 16, no. 3 (September 1987): 111–15. http://dx.doi.org/10.1177/003072708701600303.

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Recombinant DNA technology covers a wide range of biochemical techniques used to cut, splice, and move DNA from one organism to another. Genetic engineering began as a basic scientific study to learn more about gene expression and gene structure in bacteria. In the last 10 years the techniques of recombinant DNA technology have moved from the university research laboratory to the industrial production level. The techniques are applicable to all organisms and studies have been made of the genomes of viruses, bacteria, yeasts, animals, and plants. It is the latter, genetic engineering of plants, which is covered in this article.
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Bogomolova, E. G., P. M. Kopeykin, and A. A. Tagaev. "Genetic engineering approaches to the development of modern therapeutics." Medical academic journal 20, no. 3 (September 15, 2020): 49–60. http://dx.doi.org/10.17816/maj34092.

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The classic approach to production of protein-based therapeutics is their isolation from natural sources. This approach was associated with a number of difficulties, such as collecting the primary material from natural sources, isolating and purifying the protein, and its standardizing. With the development of recombinant DNA technology, itbecame possible to obtain large quantities of protein preparations lacking any contaminations. Human insulin produced using recombinant DNA technology is the first commercial therapeutic obtained by this way. Due to the rapid development of genetic engineering technologies, a large number of proteins have been obtained inEscherichia colicells. In recent years, the approach for the development of drugs based on DNA molecules containing genes encoding therapeutic proteins has been developing more actively. Today, many scientists believe in the prospects of application of DNA vaccines. The ease of production, stability, the ability to mimic natural infections and elicit appropriate immune responses make this vaccine platform extremely attractive. Delivery and targeting of immunologically relevant cells are major tasks for maximizing the immunogenicity of DNA vaccines. Several different approaches that are currently being used to achieve this goal are discussed in this review. Pharmaceuticals based on nucleic acids have a number of undeniable advantages. The main options for prophylactic RNA vaccines, the methods used to deliver RNA to the cell, and methods for increasing the effectiveness of RNA vaccines are discussed. Usage of therapeutic drugs based on protein molecules and low molecular weight compounds is complicated by the fact that they cannot be targeted at a specific gene or its protein product, responsible for the occurrence of the disease. Action of nucleic acids can be directly directed to a particular DNA region in order to edit its nucleotide sequence. This method allows to correct a genetic defect, eliminating the cause of the disease. The principles of gene therapy and the successes achieved in this area are discussed. This review summarizes current achievements in the development of drugs based on recombinant proteins and nucleic acids.
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Ljubijankic, Goran. "Genetic engineering: from a clone to a protein." Genetika 34, no. 2-3 (2002): 73–84. http://dx.doi.org/10.2298/gensr0203073l.

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October 15, 1980, was a date when the highest increase of the share price of one company was recorded on the world most powerful New York change - rates of shares of the company Genetech jumped by more than 2.5 times in only 20 minutes. This event was a grand entrance of a new technology into the global economy and it irreversibly established a brand new status of biology within the development of civilization. Genetech is one of the first companies within the field of molecular biotechnology that accomplished such an enormous commercial success no more than seven years upon the last discoveries in the series of scientific findings that had provided formulation of a new technology designated as genetic engineering or a recombinant DNA technology. Today, 20 years later, it can be rightfully claimed that expectations of the new technology are fulfilled: even if the qualitative shift it provided to fundamental research is disregarded, its direct commercial effects are very convincing - the total value of sold products produced by this technology exceeded the sum of 60 billions dollars in 2000. Let us cite market parameters of only some products manufactured by the application of genetic engineering: annual global consumption of recombinant human insulin amounts to approximately 4.6 tons only in the industrialized part of the world, while the annual sale of recombinant human erythropoietin, interferon's and a human growth hormone amount to 2, i.e. 1.5 billions dollars, respectively.
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Duster, Troy. "Cline's Recombinant DNA Experiment as Political Rashomon." Politics and the Life Sciences 6, no. 1 (August 1987): 15–18. http://dx.doi.org/10.1017/s0730938400002677.

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Robin and Markle's “Let No One Split Asunder: Controversy in Human Genetic Engineering” is a notable contribution to the sociology of science and a clarification of some important social and political issues in the current controversy around the new recombinant DNA technologies. Their four-fold taxonomy of levels of inquiry and analysis is a theoretical advance. It is also a device for illuminating, decoding, deciphering, and finally for bettering our understanding of what happened structurally in the Cline case. Much of the contemporary work in the sociology of science touches on a few, or even several of these dimensions, but because the analytic categories are frequently (if inadvertently) run together, one is often burdened with a confusing vault between levels. (Few authors make the analytic distinctions of different levels as do Robin and Markle, so it is hardly surprising that they would “run them together.”)
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ZOLLER, M. "New recombinant DNA methodology for protein engineering." Current Biology 2, no. 9 (September 1992): 510. http://dx.doi.org/10.1016/0960-9822(92)90692-4.

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MUYSSON, DAVID J., and ANN M. VERRINDER GIBBINS. "THE ALTERATION OF MILK CONTENT BY GENETIC ENGINEERING AND RECOMBINANT DNA-MEDIATED SELECTION TECHNIQUES." Canadian Journal of Animal Science 69, no. 3 (September 1, 1989): 517–27. http://dx.doi.org/10.4141/cjas89-064.

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Since the advent of gene cloning technology, suggestions have been made of specific alterations that could be engineered into the genomes of cattle, sheep or goats to alter their milk composition. These alterations could affect milk protein characteristics or quantity in ways which might be of benefit to milk processing and dairy product manufacturing industries. Other changes could improve the suitability of bovine milk for human consumption. In addition, animals could be engineered to synthesize valuable foreign proteins, such as pharmaceuticals, to be secreted in milk. In this paper, we evaluate these suggestions critically, taking into account current understanding of milk composition and processing, as well as recent information concerning the structure and regulation of genes coding for proteins involved in determining milk content. All these suggested alterations depend on successful production of transgenic animals capable of efficiently expressing introduced genes. The view is examined that some improvements in type or amount of certain milk proteins might better be achieved by conventional breeding practices, or by breeding systems that would rely on recombinant DNA techniques for methods of selection. Key words: Casein, beta-lactoglobulin, α-lactalbumin, plasmin, transgenic, bovine, ovine, lactose
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Biagetti, Marco, Francesca Vitellozzi, and Carla Ceoloni. "Physical mapping of wheat-Aegilops longissima breakpoints in mildew-resistant recombinant lines using FISH with highly repeated and low-copy DNA probes." Genome 42, no. 5 (October 1, 1999): 1013–19. http://dx.doi.org/10.1139/g98-172.

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Fluorescence in situ hybridization (FISH) with multiple probes, consisting of highly repeated DNA sequences (pSc119.2 and pAs1) and of a low-copy, 3BS-specific RFLP sequence (PSR907), enabled determination of the physical position of the wheat-alien breakpoints (BPs) along the 3BS and 3DS arms of common wheat recombinant lines. These lines harbour 3SlS Aegilops longissima segments containing the powdery mildew resistance gene Pm13. In all 3B recombinants, the wheat-Aegilops longissima physical BPs lie within the interval separating the two most distal of the three pSc119.2 3BS sites. In all such recombinants a telomeric segment, containing the most distal of the pSc119.2 3BS sites, was in fact replaced by a homoeologous Ae. longissima segment, marked by characteristic pSc119.2 hybridization sites. Employment of the PSR907 RFLP probe as a FISH marker allowed to resolve further the critical region in the various 3B recombinant lines. Three of them, like the control common wheat, exhibited between the two most distal pSc119.2 sites a single PSR907 FISH site, which was missing in a fourth recombinant line. The amount of alien chromatin can thus be estimated to represent around 20% of the recombinant arm in the three former lines and a maximum of 27% in the latter. A similar physical length was calculated for the alien segment contained in three 3D recombinants, all characterized by the presence of the Ae. longissima pSc119.2 sites distal to the nearly telomeric pAs1 sites of normal 3DS. Comparison between the FISH-based maps and previously developed RFLP maps of the 3BS-3SlS and 3DS-3SlS arms revealed substantial differences between physical and genetic map positions of the wheat-alien BPs and of molecular markers associated with the critical chromosomal portions.Key words: wheat-alien recombinants, chromosome engineering, fluorescence in situ hybridization, highly repeated and low-copy DNA probes, physical versus genetic maps.
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Bernstein, R. L., and Sheldon Krimsky. "Genetic Alchemy, the Social History of the Recombinant DNA Controversy." Leonardo 18, no. 2 (1985): 121. http://dx.doi.org/10.2307/1577892.

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Tirrell, David A., Maurille J. Fournier, and Thomas L. Mason. "Genetic Engineering of Polymeric Materials." MRS Bulletin 16, no. 7 (July 1991): 23–28. http://dx.doi.org/10.1557/s0883769400056505.

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Polymerization reactions are generally divided into two broad classes: step growth or polycondensation reactions (examples would include the synthesis of polyamides and polyesters), and chain growth processes such as those used to prepare polyethylene or polystyrene. These processes are illustrated schematically in Figure 1.The statistical nature of step and chain growth polymerization processes ensures that the products of such reactions must be heterogeneous. Conventional polymeric materials thus consist of mixtures of chains, often characterized by relatively broad distributions of chain length or composition. In many materials applications, this kind of molecular heterogeneity is advantageous since it suppresses crystallization and helps to preserve desirable properties such as optical clarity or elasticity. On the other hand, synthetic developments that afford improved control of macromolecular architecture have had profound impact on materials science and technology. As examples, one can cite the discovery of Ziegler-Natta polymerization, now used to prepare billions of pounds per year of crystalline polyolefins, or the development of living anionic polymerization of olefins, which led directly to block copolymers and the commercially important thermoplastic elastomers.The advent of recombinant DNA methods has provided a basis for developing polymeric materials characterized by essentially absolute uniformity of chain length, sequence, and stereochemistry. This article outlines the principles governing the cloning and expression of artificial genes, and examines the potential role of artificial proteins in polymer materials science.
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Dissertations / Theses on the topic "Genetic engineering Recombinant DNA"

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黃雅誼 and Nga-yi Queenie Wong. "DNA engineering utilizing thymidylate synthase A (thyA) selection system in Escherichia coli." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B31226851.

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Wong, Nga-yi Queenie. "DNA engineering utilizing thymidylate synthase A (thyA) selection system in Escherichia coli /." Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?

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Daum, Marilyn. "Geneplanner : a prototype of an expert system to assist with chemical DNA gene synthesis planning /." Online version of thesis, 1989. http://hdl.handle.net/1850/10576.

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Kepple, Kevin V. "Analysis of the binding mechanisms and cellular targets of peptide inhibitors that block site-specific recombination in vitro /." Diss., Connect to a 24 p. preview or request complete full text in PDF formate. Access restricted to UC campuses, 2006. http://wwwlib.umi.com/cr/ucsd/fullcit?p3208620.

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Comeaux, Jay Louis. "Transfer of plasmids by genetically-engineered Erwinia carotovora." Thesis, Virginia Tech, 1989. http://hdl.handle.net/10919/45933.

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The ability of a genetically-engineered Erwirzia carotovora subsp. carotovora (Ecc) strain to transfer recombinant chromosomal DNA or plasmids to wildtype Ecc or Pseudomonas fluorescens was tested on filters, within soil microcosms, and in planta. Ecc was engineered by chromosomal insertion of a disarmed endo-pectate lyase gene marked with a 1.4kb DNA fragment conferring kanamycin resistance. Plasmids RPI and pBR322 were introduced separately into engineered Ecc clones. These strains served as donors in genetic transfer experiments. No transfer of the inserted kan marker or of pBR322 was observed under any experimental condition. In filter matings, RPI was transferred to wildtype Ecc at a frequency of 3.6 X 10-2 transconjugants per donor (TPD) and to P. fluorescens at a frequency of 2.4 X 10-5 TPD. In matings conducted in potato tubers inoculated using sewing needles, the respective frequencies were 4.0 X 10-4 and 2.0 X 10-4, while matings on potato slices yielded frequencies of 4.7 X 10-2 and 2.3 X 10-2. In soil microcosms, the maximum transfer frequencies observed were 2.3 X 10-3 and 8.4 X 10-5 TPD.


Master of Science
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Dambros, Régia Maria Feltrin. "Amplificação, clonagem e expressão de proteína recombinante do vírus da doença de Aujeszky em sistema de baculovírus para utilização em programa de controle e erradicação." Universidade do Estado de Santa Catarina, 2006. http://tede.udesc.br/handle/handle/966.

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Aujeszky s disease (AD) is an infect-contagious illness that causes serious economical damages to the producer and the swine industry. Aiming to develop mechanisms and to improve technologies that are faster, more sensitive and more specific for diagnosis and for use in free areas or in AD s eradication programs, the sequence codifier of the glycoprotein E (gE) of Aujeszky s disease virus (ADV) was amplified, cloned and expressed. Through genetic engineering the sequence of the gE gene was propagated in an host organism. The gE was amplified by the technique of polimerase chain reaction (PCR), cloned in the vector pGem®-T Easy and transformed in competent cells of Escherichia coli, DH-5a . The clone obtained was sub-cloned in the expression plasmid pFastBac 1, which contains the promoter gene of the polyhedrin. The obtained subclone was analyzed inside for certification of its correct plasmid orientation with the restriction endonucleases BamH I and EcoR I. The sub-clone with the correct orientation had its DNA extracted and used for transposition inside the bacmid (recombinant baculovirus and helper plasmid with competent DH10Bac cell). Colonies with inserted gE were selected by the phenotype of the colony, which expresses white color when cloned. White recombinant colonies had their DNA extracted and used for cotransfection in insect cells Trichoplusia ni (BTI-Tn5B1-4). The recombinant-gE baculovirus was inoculated in cultured cells and expressed the recombinant gE by PCR and Western blotting . The recombinant-gE baculovirus containing only the gE gene of the VDA will be used for antigen and monoclonal antibodies production, which will aid in the development of a more sensitive, specific and safer for the use in VDA free regions
A doença de Aujeszky (DA) é uma enfermidade infecto-contagiosa que causa graves prejuízos econômicos ao produtor e à agroindústria suinícola. Com o objetivo de desenvolver insumos e aprimorar tecnologias que sejam mais rápidas, sensíveis e específicas de diagnóstico para uso em regiões livres ou em erradicação da DA, a seqüência codificadora da glicoproteína E (gE) do vírus da doença de Aujeszky (VDA) foi amplificada, clonada e expressada. Através da engenharia genética a seqüência do gene da gE foi propagada em um organismo hospedeiro. A gE foi amplificada pela técnica da reação em cadeia da polimerase (PCR) , clonada no vetor pGem®-T Easy e transformada em células competentes de Escherichia coli, DH-5a . O clone obtido foi subclonado no plasmídeo de expressão pFastBac 1, o qual possui o sítio promotor do gene da poliedrina. O subclone obtido foi analisado para certificação de sua orientação correta dentro do plasmídeo com as endonucleases de restrição BamH I e EcoR I. O subclone com a orientação correta teve seu DNA extraído e usado para a transposição dentro do bacmid (baculovírus recombinante e plasmídeo helper em célula competente DH10Bac ). As colônias com inserto gE foram selecionadas pelo fenótipo da colônia, a qual expressa cor branca quando clonada. As colônias brancas recombinantes tiveram seu DNA extraído e usado para a co-transfecção em células do inseto Trichoplusia ni (BTITn5B1- 4). O baculovírus gE-recombinante ao ser inoculado em cultivo celular, expressou a gE recombinante, comprovada pela técnica de PCR e Western blotting . O baculovírus gE-recombinante contendo apenas o gene da gE do VDA será utilizado para produção de antígeno e de anticorpos monoclonais, o que auxiliará no desenvolvimento de um teste de diagnóstico mais sensível, específico e mais seguro para uso em áreas livres do VDA
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MURATA, YOKO. "Sintese e caracterizacao do hormonio tireotrofico humano recombinante (rec-hTSH) contendo uma sub unidade beta quimerica (rec-hTSHbeta-CTEP hCGbeta)." reponame:Repositório Institucional do IPEN, 1995. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10432.

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IPEN/T
Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP
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MATHOR, MONICA B. "Estudos da expressao genica mediante utilizacao de queratinocitos humanos normais transduzidos com o gene do hormonio de crescimento humano .Possivel utilizacao em terapia genica." reponame:Repositório Institucional do IPEN, 1994. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10410.

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Tese (Doutoramento)
IPEN/T
Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP
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Cousseau, Philippe. "Traitement d'image et traitement graphique en genie genetique : application a l'analyse d'images autoradiographiques et a la representation de cartes de molecules d'adn recombinant." Université Louis Pasteur (Strasbourg) (1971-2008), 1986. http://www.theses.fr/1986STR13037.

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La premiere application concerne la lecture automatique des images autoradiographiques de gel d'electrophorese bidimensionnelle de proteines. La seconde application porte sur la realisation d'un logiciel interactif d'aide au dessin assiste par ordinateur, specifiquement dedie a laconstruction et a la representation graphique de cartes de molecules d'adn recombinants
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Jones, Mary Ellen. "Politically Corrected Science: The Early Negotiation of U.S. Agricultural Biotechnology Policy." Diss., Virginia Tech, 1999. http://hdl.handle.net/10919/29868.

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This social history of science policy development emphasizes the impact on the agricultural community of federal policies regarding release of recombinant DNA (rDNA) organisms into the environment. The history also demonstrates that the U.S. Coordinated Framework for Biotechnology Regulation (1986) is based principally in political criteria, not solidly based in science as its proponents claimed. The power struggle among policy negotiators with incompatible belief systems resulted in a political correction of biotechnology. I also demonstrate that episodes in the rDNA controversy occur in repetitive and periodic patterns. During the 1980s, the first rDNA microbial pesticide, Ice-Minus, struggled through a policy gauntlet of federal agency approval processes, a Congressional hearing, and many legal actions before it was finally released into the environment. At the height of the controversy (1984-1986), the Reagan Administration would admit no new laws or regulations to slow the development of technologies or hinder American international competitiveness. At the same time, Jeremy Rifkin, a radical activist representing a green world view, used the controversy to agitate for social and economic reform. Meanwhile, a group of Congressional aides who called themselves the "Cloneheads" used the debate to fight for more public participation in the science policy-making process. Conflicting perspectives regarding biotechnology originated, not in level of understanding of the science involved, but in personal perspectives that were outwardly expressed as political group affiliations. The direction of federal biotechnology policy was influenced most successfully by politically best-positioned individuals (what I call a "hierarchy effect") who based decisions on how biotechnology harmonized with their pre-existing beliefs. The success of their actions also depended on timing. Historical events during the rDNA controversy followed the same periodic pattern--gestation, threshold, crisis/conflict, and quasi-quiescence--through two consecutive eras--the Containment Era (1970s) and the Release Era (1980s). These periods are modeled after Fletcher's stages through which ethical issues evolve (1990). However, an agricultural perspective on the debate reveals that such stages also occur in finer detail on repeating, overlapping, and multi-level scales. Knowledge of this periodicity may be useful in predicting features of future episodes of the rDNA controversy.
Ph. D.
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Books on the topic "Genetic engineering Recombinant DNA"

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Adrianne, Massey, ed. Recombinant DNA and biotechnology. Washington, D.C: ASM Press, 1996.

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B, Primrose S., ed. Principles of gene manipulation: An introduction to genetic engineering. 3rd ed. Oxford: Blackwell Scientific Publications, 1985.

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B, Primrose S., ed. Principles of gene manipulation: An introduction to genetic engineering. 5th ed. Oxford: Blackwell Scientific, 1994.

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B, Primrose S., ed. Principles of gene manipulation: An introduction to genetic engineering. 4th ed. Oxford [England]: Blackwell Scientific Publications, 1989.

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M, Twyman Richard, and Old R. W, eds. Principles of gene manipulation. 6th ed. Oxford: Blackwell Scientific, 2001.

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J, Pasternak Jack, and Patten Cheryl L, eds. Molecular biotechnology: Principles and applications of recombinant DNA. 4th ed. Washington, DC: ASM Press, 2010.

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Recombinant DNA basics: A primer : programmed learning. Denver, CO: Berkeley Scientific Publications, 1993.

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Kreuzer, Helen. Recombinant DNA and biotechnology: A guide for students. 2nd ed. Washington, D.C: ASM Press, 2001.

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Adrianne, Massey, ed. Recombinant DNA and biotechnology: A guide for teachers. 2nd ed. Washington, D.C: ASM Press, 2001.

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G, Williams J. Genetic engineering. Oxford: BIOS Scientific Publishers, 1993.

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Book chapters on the topic "Genetic engineering Recombinant DNA"

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Young, Richard A., and Ronald W. Davis. "Immunoscreening λgtll Recombinant DNA Expression Libraries." In Genetic Engineering: Principles and Methods, 29–41. Boston, MA: Springer US, 1985. http://dx.doi.org/10.1007/978-1-4684-4973-0_2.

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Luciw, Paul, Debbie Parkes, Gary Van Nest, Dino Dina, Kathleen Hendrix, and Murray B. Gardner. "Recombinant DNA Approaches to Feline Leukemia Virus Immunization." In Genetic Engineering of Animals, 207–15. Boston, MA: Springer US, 1986. http://dx.doi.org/10.1007/978-1-4684-5110-8_18.

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Akram, Muhammad, Farhat Jabeen, Muhammad Daniyal, Rida Zainab, Usman ul Haq, Charles Oluwaseun Adetunji, Chukwuebuka Egbuna, Benson C. Ephraim-Emmanuel, Kingsley C. Patrick-Iwuanyanwu, and Ahiakwo B. Ogbo. "Genetic Engineering of Novel Products of Health Significance: Recombinant DNA Technology." In Functional Foods and Nutraceuticals, 595–611. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-42319-3_26.

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Simpson, J., and L. Herrera-Estrella. "DNA Recombinants and Transformation of Agricultural Crops." In Plant Protoplasts and Genetic Engineering II, 75–98. Berlin, Heidelberg: Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-74454-9_4.

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Sederoff, Ronald, Anne-Marie Stomp, Babette Gwynn, Elizabeth Ford, Carol Loopstra, Paul Hodgskiss, and W. Scott Chilton. "Application of Recombinant DNA Techniques to Pines: A Molecular Approach To Genetic Engineering in Forestry." In Cell and Tissue Culture in Forestry, 314–29. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-017-0994-1_19.

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Grompe, Marcus, Wade Johnson, and J. Larry Jameson. "Recombinant DNA and Genetic Techniques." In Principles of Molecular Medicine, 9–24. Totowa, NJ: Humana Press, 1998. http://dx.doi.org/10.1007/978-1-59259-726-0_2.

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Razin, Aharon. "DNA Methylases." In Genetic Engineering, 1–11. Boston, MA: Springer US, 1989. http://dx.doi.org/10.1007/978-1-4615-7084-4_1.

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Blanchard, Alan. "Synthetic DNA Arrays." In Genetic Engineering, 111–23. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4899-1739-3_5.

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Priyadarshini, Anjali, and Prerna Pandey. "Genetic Manipulation by Recombinant DNA Technology." In Molecular Biology, 219–74. Toronto ; New Jersey : Apple Academic Press, 2018.: Apple Academic Press, 2018. http://dx.doi.org/10.1201/b22354-5.

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Hochuli, Erich. "Purification of Recombinant Proteins with Metal Chelate Adsorbent." In Genetic Engineering, 87–98. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4613-0641-2_6.

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Conference papers on the topic "Genetic engineering Recombinant DNA"

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Junid, Syed Abdul Mutalib Al, Muhd Shahirad Reffin, Zulkifi Abd Majid, Nooritawati Md Tahir, and Muhammad Adib Haron. "Implementation of genetic algorithm for optimizing DNA sequence alignment." In 2012 IEEE Business Engineering and Industrial Applications Colloquium (BEIAC). IEEE, 2012. http://dx.doi.org/10.1109/beiac.2012.6226111.

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Hussien, Hany H. "DNA Computing for RGB image Encryption with Genetic Algorithm." In 2019 14th International Conference on Computer Engineering and Systems (ICCES). IEEE, 2019. http://dx.doi.org/10.1109/icces48960.2019.9068136.

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Muttakin, Al, and Mohammad Rezwanul Huq. "Motif discovery in unaligned DNA sequences using genetic algorithm." In 2017 4th International Conference on Advances in Electrical Engineering (ICAEE ). IEEE, 2017. http://dx.doi.org/10.1109/icaee.2017.8255450.

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Peng, Xinmei, Xuedong Zheng, Bin Wang, Changjun Zhou, and Qiang Zhang. "A micro-genetic algorithm for DNA encoding sequences design." In 2016 2nd International Conference on Control Science and Systems Engineering (ICCSSE). IEEE, 2016. http://dx.doi.org/10.1109/ccsse.2016.7784342.

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Liu, Xiaohong. "Notice of Retraction: A Report on the Teaching Contents and Methods of DNA Recombinant Technology in the Course Molecular Biology." In 2011 5th International Conference on Bioinformatics and Biomedical Engineering. IEEE, 2011. http://dx.doi.org/10.1109/icbbe.2011.5780096.

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Tremberger, George, S. Dehipawala, A. Nguyen, E. Cheung, R. Sullivan, T. Holden, D. Lieberman, and T. Cheung. "Complexity of genetic sequences modified by horizontal gene transfer and degraded-DNA uptake." In SPIE Optical Engineering + Applications, edited by Richard B. Hoover, Gilbert V. Levin, Alexei Yu Rozanov, and Nalin C. Wickramasinghe. SPIE, 2015. http://dx.doi.org/10.1117/12.2186550.

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Chen Xiao and Zhang Ridong. "A DNA genetic algorithm with reconstruct operator for chemical process modeling." In 2011 Second International Conference on Mechanic Automation and Control Engineering (MACE). IEEE, 2011. http://dx.doi.org/10.1109/mace.2011.5987087.

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Guo, Y. C., H. Wang, and B. L. Zhang. "Blind equalization algorithms based on DNA genetic optimization of artificial fish swarm." In International Conference on Automation, Mechanical and Electrical Engineering. Southampton, UK: WIT Press, 2014. http://dx.doi.org/10.2495/amee140922.

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Qiu, Weiguo, Arjun Stokes, Joseph Cappello, and Xiaoyi Wu. "Electrospinning of Recombinant Protein Polymer Nanofibers." In ASME 2009 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2009. http://dx.doi.org/10.1115/sbc2009-206352.

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Abstract:
Structural proteins often in the form of micro and nanofibers, constituting most of intra- and extracellular matrix (ECM), are the fundamental building blocks of life [1]. Recent efforts to replace diseased or damaged tissues and organs have resulted in the molecular design and genetic engineering of recombinant proteins, and the advent of new technology for fabricating structural proteins into micro-/nanofibrous scaffolds, hoping to resemble some or all the characteristics of ECM structure and function. The fabrication of such an ECM mimic may be an important step in engineering a functional tissue. To this end, we have produced a series of silk-elastin-like proteins (SELPs) [2]. Revealed by our subsequent studies, SELPs in the form of hydrogels, thin films, and microfibers, have displayed a set of outstanding biological and physical properties. In this study, electrospinning will be pursued as a mechanism for the formation of SELP nanofibers.
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Wu, Huapeng, and Yecai Guo. "DNA Genetic Optimization Bat Algorithm Based Fractionally Spaced Multi-modulus Blind Equalization Algorithm." In 2015 International Industrial Informatics and Computer Engineering Conference. Paris, France: Atlantis Press, 2015. http://dx.doi.org/10.2991/iiicec-15.2015.133.

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Reports on the topic "Genetic engineering Recombinant DNA"

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Beachy, R. N. [Genetic engineering with a gene encoding a soybean storage protein to identify DNA sequences to control its expression]: Annual report, 1993. Office of Scientific and Technical Information (OSTI), December 1993. http://dx.doi.org/10.2172/10133802.

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