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Journal articles on the topic 'Genetic intra-specific variability'

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1

CARVALHO, O. S., R. L. CALDEIRA, A. J. G. SIMPSON, and T. H. D. A. VIDIGAL. "Genetic variability and molecular identification of Brazilian Biomphalaria species (Mollusca: Planorbidae)." Parasitology 123, no. 7 (2001): 197–209. http://dx.doi.org/10.1017/s0031182001008058.

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Freshwater snails belonging to the genus Biomphalaria are intermediate hosts of the trematode Schistosoma mansoni in the Neotropical region and Africa. In Brazil, one subspecies and ten species of Biomphalaria have been identified: B. glabrata, B. tenagophila, B. straminea, B. occidentalis, B. peregrina, B. kuhniana, B. schrammi, B. amazonica, B. oligoza, B. intermedia and B.t. guaibensis. However, only the first three species are found naturally infected with S. mansoni. The classical identification of these planorbids is based on comparison of morphological characteristics of the shell and m
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Farahani, Farah, Masoud Sheidai, and Fahimeh Koohdar. "Genetic finger printing of cotton cultivars by ISSR molecular markers." Genetika 50, no. 2 (2018): 627–34. http://dx.doi.org/10.2298/gensr1802627f.

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Gossypium hirsutum is one of the main tetraploid cotton species that is cultivated throughout the world. Due to continuous selection of cotton cultivars for specific agronomic traits, the genetic variability within the cultivars decrease that lead to genetic erosion. To tackle the problem of reduced genetic variability, we should track all available genetic diversity within cotton germplasm and use them for inter-specific and intra-specific hybridization and produce new elite cotton cultivars. Therefore, the present study used ISSR molecular markers to illustrate genetic variability in 13 tetr
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Talman, Arthur M., Linda Duval, Eric Legrand, et al. "Evaluation of the intra- and inter-specific genetic variability of Plasmodium lactate dehydrogenase." Malaria Journal 6, no. 1 (2007): 140. http://dx.doi.org/10.1186/1475-2875-6-140.

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Mossi, AJ, RL Cansian, O. Leontiev-Orlov, et al. "Intra and inter populational genetic variability in Maytenus ilicifolia Mart. ex Reiss. 1861, through RAPD markers." Brazilian Journal of Biology 67, no. 4 suppl (2007): 957–61. http://dx.doi.org/10.1590/s1519-69842007000500022.

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Maytenus ilicifolia is a medicinal plant largely used in the South Brazilian folk medicine. The aim of this study was to quantify the intra and inter populational genetic variability in three populations of M. ilicifolia, focusing on the genetic conservation of this species, which has been threatened by anthropic action. RAPD (Random Amplified Polymorphic DNA) markers were used to analyze 30 plants of each of the three populations collected in the Alto Uruguai Gaúcho region. Fourteen selected primers generated a total of 158 bands, 71.5% of which were polymorphic. The comparison of Jaccard’s d
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J., O. Popoola, M. Adebayo B., E. Adegbite A., C. Omonhinmin A., and D. Adewale B. "Fruit Morphometric and RAPD Evaluation of Intraspecific Variability in Some Accessions of African Yam Bean (Sphenostylis stenocarpa Hochst. ex. A. Rich. Harms)." Annual Research & Review in Biology 14, no. 4 (2017): 1–10. https://doi.org/10.9734/ARRB/2017/34264.

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African Yam Bean (AYB) (<em>Sphenostylis stenocarp</em>a, Hochst. ex A. Rich, Harms) is an indigenous underutilized legume mainly grown in Sub-saharan African as a source of protein. Intraspecific variability studies were carried out on 10 accessions of AYB obtained from the International Institute of Tropical Agriculture (IITA) Ibadan, Nigeria. Fourteen (14) fruit morphometric characters and nine (9) arbitrary RAPD primers were employed for evaluation of genetic intra-specific variability among the accessions. A total of 410 bands were generated with 261 (63.66%) polymorphic bands. There is s
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Rocha, D. C., R. A. Rodella, C. L. Marino, and D. Martins. "Genetic variability among Commelina weed species from the states of Paraná and São Paulo, Brazil." Planta Daninha 27, no. 3 (2009): 421–27. http://dx.doi.org/10.1590/s0100-83582009000300001.

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This work aims to carry out a comparative analysis using RAPD molecular markers in four Commelina weed species from the state of Paraná and C. benghalensis populations from the states of Paraná and São Paulo, Brazil. The genomic plant DNA sample was extracted from the leaves, separated, randomly fragmented and amplified by PCR. Random amplified polymorphic DNA fragments (RAPD markers) were analyzed by using POPGENE statistical program. Eighty-five primer sequences were tested but only three were suitable as molecular markers producing 37 DNA polymorphic fragments for comparisons among four Com
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Vejl, P., S. Skupinová, I. Polišenská, and M. Váňová. "Distinguishing isolates of cereal stem disease pathogens of the genus Tapesia from isolates of Fusarium and Rhizoctonia using the RAPD method." Plant Protection Science 36, No. 4 (2000): 132–40. http://dx.doi.org/10.17221/9645-pps.

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The RAPD method (Random Amplification of Polymorphous DNA) was used to distinguish Tapesia acufonnis and T. yallundae isolates from other pathogens of wheat stem base diseases (Fusarium avenaceum, F. culmorum, F. nivale, Rhizoctonia cerealis and R. so/ani).Isolates of the fungi originated from infected wheat plants (Triticum aestivum L.) collected at various locations in the Czech Republic. Three decameric oligonucleotide-primers were selected as producing genotype-specific RAPD products. These markers distinguish all isolates of the above taxons. The intra-species genetic variability of these
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Attia, Hanene, Manel Sghaier, Aymen Bali, et al. "Intra-Specific Diversity of Leishmania major Isolates: A Key Determinant of Tunisian Zoonotic Cutaneous Leishmaniasis Clinical Polymorphism." Microorganisms 10, no. 3 (2022): 505. http://dx.doi.org/10.3390/microorganisms10030505.

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The clinical expression of zoonotic cutaneous leishmaniasis (ZCL) caused by Leishmania (L.) major parasites has a broad spectrum ranging from asymptomatic infection to self-limited cutaneous sores or severe disease. In concert with the host immune responses, the vector variability and the number of bites, genetic variation between L. major isolates might impact on the clinical output of the disease. We investigated herein the intra-specific variability of L. major field isolates independently of host or vector factors and then tried to correlate parasite variability to ZCL severity in correspo
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Buha, Natasa, Milica Gligorovic, and Jasmina Maksic. "Challenging behavior: Behavioral phenotypes of some genetic syndromes." Srpski arhiv za celokupno lekarstvo 142, no. 9-10 (2014): 621–27. http://dx.doi.org/10.2298/sarh1410621b.

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Challenging behavior in individuals with mental retardation (MR) is relatively frequent, and represents a significant obstacle to adaptive skills. The frequency of specific forms and manifestations of challenging behavior can depend on a variety of personal and environmental factors. There are several prominent theoretical models regarding the etiology of challenging behavior and psychopathology in persons with MR: behavioral, developmental, socio-cultural and biological. The biological model emphasizes the physiological, biochemical and genetic factors as the potential source of challenging b
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Delaneau, O., M. Zazhytska, C. Borel, et al. "Chromatin three-dimensional interactions mediate genetic effects on gene expression." Science 364, no. 6439 (2019): eaat8266. http://dx.doi.org/10.1126/science.aat8266.

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Studying the genetic basis of gene expression and chromatin organization is key to characterizing the effect of genetic variability on the function and structure of the human genome. Here we unravel how genetic variation perturbs gene regulation using a dataset combining activity of regulatory elements, gene expression, and genetic variants across 317 individuals and two cell types. We show that variability in regulatory activity is structured at the intra- and interchromosomal levels within 12,583 cis-regulatory domains and 30 trans-regulatory hubs that highly reflect the local (that is, topo
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Cervera, M. T., D. Remington, J. M. Frigerio, et al. "Improved AFLP analysis of tree species." Canadian Journal of Forest Research 30, no. 10 (2000): 1608–16. http://dx.doi.org/10.1139/x00-085.

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Amplified fragment length polymorphism (AFLP) is a high-throughput, molecular-marker technique that is used increasingly in a variety of genetic analyses. Here, the conditions for carrying out AFLP analysis have been established for different tree species, including both angiosperm and gymnosperm trees, with genome sizes ranging from 0.54 to 38 pg DNA/2C. Specific parameters have been determined to provide informative and reproducible AFLP fingerprints of peach (Prunus persica L.), eucalypt, oak, poplar, and loblolly pine (Pinus taeda L.). Typically, 80-130 amplified DNA fragments (i.e., loci
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LAL, RAJ K., CHANDAN S. CHANOTIYA, SUNITA S. DHAWAN, et al. "Estimation of intra-specific genetic variability and half-sib family selection using AMMI (Additive Main Effects and Multiplicative Interactions) model in menthol mint (Mentha arvensis L.)." Journal of Medicinal and Aromatic Plant Sciences 42, no. 2 (2020): 102–13. http://dx.doi.org/10.62029/jmaps.v42i2.lal.

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Batos, Branislava, Danijela Miljkovic, and Jelena Ninic-Todorovic. "Length of vegetation period as parameter of common oak (Quercus robur L.) phenological variability." Genetika 44, no. 1 (2012): 139–52. http://dx.doi.org/10.2298/gensr1201139b.

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Paper presents results for length of vegetation period as factor in common oak?s (Quercus robur L.) phenological variability. Trees in two common oaks populations on two locations in area of Belgrade were researched. Average length of vegetation period on site Ada Ciganlija is 166 days, while in location Bojcinska suma is 179 days. Regarding that populations are in similar environmental and habitual conditions, differences in results should be treated as consequence of common oaks intra-specific variability and genetic structure of population. Significant differences in three consecutive years
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Maskromo, Ismail, Azis Natawijaya, Syafaruddin Syafaruddin, Fadjry Djufri, and M. Syakir. "Variabilitas Genetik Plasma Nutfah Kelapa Sawit Asal Angola dan Seleksi Genotipe Berbasis Famili dan Individu untuk Pembentukan Breeding Population Baru." Buletin Palma 18, no. 1 (2017): 43. http://dx.doi.org/10.21082/bp.v18n1.2017.43-51.

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&lt;p class="Default"&gt;Development a new oil palm variety is determined by the availablity of oil palm genetic materials. The genetic variability could be resulted from both intra and inter family variation. Angola oil palm germplasm is a new oil palm material which were collected from natural habitat in Angola, Africa by Indonesian oil palm qonsortium. The objective of this research were to identify genotypes which carrying any specific characters as well as genotypes-based selection of families and individuals for the formation of a new breeding population. The research were conducted at K
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Ramdani, Messaoud, Takia Lograda, Pierre Chalard, Jean Claude Chalchat, and Gilles Figueredo. "Chemical Variability of Essential Oils in Natural Populations of Cupressus dupreziana." Natural Product Communications 6, no. 1 (2011): 1934578X1100600. http://dx.doi.org/10.1177/1934578x1100600121.

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Essential oils extracted from dried leaves of Cupressus dupreziana A. Camus, an endemic species in the Tassili n'Ajjer (Central Sahara of Algeria), were analyzed by gas chomatography coupled to mass spectrometry (GC-MS). Analyses were carried out on 164 trees of 26 natural populations in order to determine the intra-specific variability. Thirty-two terpenoïds were identified, the major ones being α-pinene (11.5 – 44.2), Δ3-carene (5.7 – 31.7) and germacrene-D (15.7 – 54.1). The terpenoid markers used made it possible to determine the individual patterns of chemotypic variability. This variabil
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Villano, Clizia, Giandomenico Corrado, Boris Basile, et al. "Morphological and Genetic Clonal Diversity within the ‘Greco Bianco’ Grapevine (Vitis vinifera L.) Variety." Plants 12, no. 3 (2023): 515. http://dx.doi.org/10.3390/plants12030515.

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Grapevine (Vitis vinifera L.) has been propagated vegetatively for hundreds of years. Therefore, plants tend to accumulate somatic mutations that can result in an intra-varietal diversity capable of generating distinct clones. Although it is common that winemakers request specific clones or selections for planting new vineyards, relatively limited information is available on the extent, degree, and morphological impact of the clonal diversity in traditional, highly valued grapevine varieties within production areas protected by geographical denomination of origin. Here, we present a morphologi
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Babicz, Marek, Barbara Danielak-Czech, Anna Kozubska-Sobocińska, et al. "Cytogenetic and molecular studies in conservation breeding of Pulawska breed pigs." Medycyna Weterynaryjna 73, no. 7 (2017): 395–98. http://dx.doi.org/10.21521/mw.5725.

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The paper presents the genetic characteristics of Pulawska breed pigs carried out on the basis of cytogenetic and molecular studies. Among chromosome markers, polymorphic variants of specific chromosome structures such as centromeric heterochromatin areas (C bands) and nucleolar organizing regions (NOR bands) were taken into account. The described breed-specific tendencies concerning size polymorphism of these structures are the source of chromosome markers that are useful for identifying the linkage with genes controlling important production traits. On the other hand, molecular studies prese
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Dimitrova, Z., D. S. Campo, S. Ramachandran, et al. "Evaluation of viral heterogeneity using next-generation sequencing, end-point limiting-dilution and mass spectrometry." In Silico Biology: Journal of Biological Systems Modeling and Multi-Scale Simulation 11, no. 5-6 (2012): 183–92. https://doi.org/10.3233/isb-2012-0453.

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Hepatitis C Virus sequence studies mainly focus on the viral amplicon containing the Hypervariable region 1 (HVR1) to obtain a sample of sequences from which several population genetics parameters can be calculated. Recent advances in sequencing methods allow for analyzing an unprecedented number of viral variants from infected patients and present a novel opportunity for understanding viral evolution, drug resistance and immune escape. In the present paper, we compared three recent technologies for amplicon analysis: (i) Next-Generation Sequencing; (ii) Clonal sequencing using End-point Limit
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Santos, Marilene Hilma dos, Rosana Rodrigues, Leandro Simões Azeredo Gonçalves, Cláudia Pombo Sudré, and Messias Gonzaga Pereira. "Agrobiodiversity in Cucurbita spp. landraces collected in Rio de Janeiro assessed by molecular markers." Crop Breeding and Applied Biotechnology 12, no. 2 (2012): 96–103. http://dx.doi.org/10.1590/s1984-70332012000200001.

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Diversity and genetic relationship in forty landraces of Cucurbita spp. collected at small farms in Rio de Janeiro, Brazil, were analyzed by RAPD and ISSR markers, using 20 and 15 primers, respectively. Both markers were efficient to cluster the accessions separating among species, but not so much to the detection of intra-specific variability, considering the event of different pairs of accessions comprising null genetic distances observed for both markers in C. moschata. Low values observed for genetic distance among the C. moschata landraces showed that most likely genetic losses is in prog
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Sudré, Cláudia Pombo, Rosana Rodrigues, Leandro Simões Azeredo Gonçalves, Ernane Ronie Martins, Messias Gonzaga Pereira, and Marilene Hilma dos Santos. "Genetic divergence among Dimorphandra spp. accessions using RAPD markers." Ciência Rural 41, no. 4 (2011): 608–13. http://dx.doi.org/10.1590/s0103-84782011005000024.

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The genus Dimorphandra has distinguish relevance considering either medicinal or biodiversity aspects because it includes two species that are economically important flavonoids sources for pharmachemical industry (D. mollis Benth. and D. gardneriana Tul.), and species endemic to Brazil, such as D. jorgei Silva and D. wilsonii Rizz., threatened by extinction. In order to evaluate variability among accessions of D. mollis (fava-d'anta), D. gardneriana and D. wilsonii, it was collected fruit from individual plants from three Brazilian states in a total of 57 accessions, which were analyzed with R
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ROBERTS, DAVID M., BRIAN BOAG, FRASER HUNTER, JAMIE TARLTON, KATRIN MACKENZIE, and ROY NEILSON. "Genetic variability of Arthurdendyus triangulatus (Dendy, 1894), a non-native invasive land planarian." Zootaxa 4808, no. 1 (2020): 38–50. http://dx.doi.org/10.11646/zootaxa.4808.1.2.

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Arthurdendyus triangulatus (Dendy, 1894) is a land planarian native to New Zealand which has become established in the United Kingdom and the Faroe Islands during the last 60 years. The species has become prevalent and widely established in Scotland mediated by human activity mostly through the exchange of plants and associated soil. As a predator of earthworms, concerns regarding both the direct impact on earthworm abundance and diversity and the indirect impact on those birds and mammals that have earthworms as a primary dietary component led to A. triangulatus being the subject of both nati
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CHEN, JIE, LUIS A. PARRA, ANDRÉ DE KESEL, et al. "Inter- and intra-specific diversity in Agaricus endoxanthus and allied species reveals a new taxon, A. punjabensis." Phytotaxa 252, no. 1 (2016): 1. http://dx.doi.org/10.11646/phytotaxa.252.1.1.

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Agaricus endoxanthus is one of the few species of Agaricus, which are broadly distributed across several continents. This typically tropical species has also been introduced and reported from European tropical greenhouses. To better circumscribe this taxon and understand its distribution, we investigated its intraspecific variability. Sixteen collections resembling A. endoxanthus from various regions were selected for morphological examination and sequencing. Through a combined morphological and molecular approach, nine specimens were identified as A. endoxanthus. No major morphological differ
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Swoboda, Ines, and Prem L. Bhalla. "RAPD analysis off genetic variation in the Australian fan flower, Scaevola." Genome 40, no. 5 (1997): 600–606. http://dx.doi.org/10.1139/g97-079.

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The use of randomly amplified polymorphic DNA (RAPD) to study genetic variability in Scaevola (family Goodeniaceae), a native Australian species used in ornamental horticulture, is demonstrated. Plants of the genus Scaevola are commonly known as "fan flowers," due to the fan-like shape of the flowers. Nineteen accessions of Scaevola (12 cultivated and 7 wild) were studied using 20 random decamer arbitrary primers. Eight primers gave a distinct reproducible amplification profile of 90 scorable polymorphic fragments, enabling the differentiation of the Scaevola accessions. RAPD amplification of
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Weising, Kurt, Dieter Kaemmer, Franz Weigand, Jörg T. Epplen, and Gunter Kahl. "Oligonucleotide fingerprinting reveals various probe-dependent levels of informativeness in chickpea (Cicer arietinum)." Genome 35, no. 3 (1992): 436–42. http://dx.doi.org/10.1139/g92-065.

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Synthetic oligonucleotides complementary to simple repetitive DNA sequences were used to detect inter- and intra-specific polymorphisms in a leguminous crop plant (chickpea, Cicer arietinum) and its wild relatives. All the investigated repetitive motifs [(GACA)4, (GATA)4, (GTG)5, (CA)8, (TCC)5, (GGAT)4, and (AGTTT)4] were abundantly present and polymorphic in the chickpea genome. Different probes revealed different levels of variability. Whereas species-specific banding patterns were obtained with the (GTG)5 probe, other probes revealed differences between accessions, or even individuals. The
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Forst, Emma, Jérôme Enjalbert, Vincent Allard, et al. "A generalized statistical framework to assess mixing ability from incomplete mixing designs using binary or higher order variety mixtures and application to wheat." Field Crops Research 242 (October 1, 2019): 107571. https://doi.org/10.1016/j.fcr.2019.107571.

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Abstract There has been recently a renewed interest for variety mixtures due to their potential capacity to stabilize production through buffering abiotic and biotic stresses. Part of this results from complementarity and/or compensation between varieties which can be assessed under mixed stands only. Mixing ability of varieties can be partitioned into <em>General</em> and <em>Specific Mixing Abilities</em> (GMA and SMA) that have been estimated so far through the evaluation of binary mixtures in complete diallel designs. However, the number of mixtures increases exponentially with the number
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MEHTA, Y. R. "Molecular and pathogenic variability of Drechslera isolates from oats." Fitopatologia Brasileira 26, no. 3 (2001): 590–96. http://dx.doi.org/10.1590/s0100-41582001000300002.

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Severe epidemics of leaf blotch and black leaf spot of oat (Avena sativa) caused by Drechslera avenae and Drechslera sp., respectively, are frequently observed in the State of Paraná, Brazil. Although some morphological differences between the isolates causing two different symptoms were noticed, the genetic relationship between them was not clear. Twenty-four isolates of D. avenae and Drechslera sp, collected between 1996-98, were assessed for the genetic variability by molecular and pathogenic analyses. The amplification products using primer pair ITS4/ITS5 showed a fragment length of approx
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Oppong, G. K., and M. D. Asante. "Genetic Diversity of Wild Rice (Oryza longistaminata) in Ghana." Agricultural and Food Science Journal of Ghana 14, no. 1 (2022): 1326–36. http://dx.doi.org/10.4314/afsjg.v14i1.1.

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Oryza longistaminata is a wild rice taxon and an excellent source of genetic variation that remains largely unexploited. This study was conducted to understand the genetic diversity between and within O. longistaminata populations, collected from different geographic locations in Ghana. In this study, SSR markers were used to determine the intra-specific variability in O. longistaminata, and how the variation is partitioned within and between different populations. Analysis of molecular variance revealed high levels of polymorphism (95.9%) within the populations. The overall genetic diversity
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Kopp, Rogério Luiz, Márcia Miyazaki, and Vanete Thomaz-Soccol. "Trypanosoma cruzi Chagas, 1909: genetic variability of isolates from chronic chagasic patients in the Paraná state, Brazil." Brazilian Archives of Biology and Technology 48, no. 3 (2005): 389–95. http://dx.doi.org/10.1590/s1516-89132005000300009.

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The present work had as objective to verify the genetic diversity among strains of Trypanosoma cruzi isolated in chronic chagasic patients in the Paraná state. Fifty patients with compatible clinical symptoms were selected (cardiopathy, megacolon and/or megaesophagus) and that presented positive serological reaction to T. cruzi. Six strains of the protozoan were isolated in hemoculture and identified by electrophoresis in starch thick gel with aid of 14 isoenzymes (6PGD; G6PD; ME¹; ME²; ICD; PGM; GPI; GOT¹; GOT²; NP¹; NP²; DIA; MPI and FH). The statistical analysis were accomplished by the sof
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Affonso, PRAM, and PM Galetti Jr. "Genetic diversity of three ornamental reef fishes (Families Pomacanthidae and Chaetodontidae) from the Brazilian coast." Brazilian Journal of Biology 67, no. 4 suppl (2007): 925–33. http://dx.doi.org/10.1590/s1519-69842007000500017.

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Reef fishes of the families Pomacanthidae (angelfish) and Chaetodontidae (butterflyfish) are popular ornamental species, intensively harvested for the aquarium trade. The impacts of such activity on intra-specific diversity and reef ecosystems are still poorly understood in the south Atlantic. In the present work, a fine-scale genetic analysis using RAPD markers was performed in distinct samples of the queen angelfish (Holacanthus ciliaris), French angelfish (Pomacanthus paru), and banded butterflyfish (Chaetodon striatus) along the Brazilian coast. Most of the genetic variation in the three s
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Moroni, Raquel Borges, Juracy de Freitas Maia, Wanderli Pedro Tadei, and Joselita Maria Mendes dos Santos. "Genetic variability among Anopheles species belonging to the Nyssorhynchus and Anopheles subgenera in the Amazon region." Revista da Sociedade Brasileira de Medicina Tropical 43, no. 4 (2010): 409–15. http://dx.doi.org/10.1590/s0037-86822010000400015.

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INTRODUCTION: Isoenzymatic analyses were performed involving species of the Nyssorhynchus and Anopheles subgenera in order to estimate the intra and interspecies genetic variability. METHODS: Mosquitoes were caught at different localities in the Amazon region. The collection and rearing of mosquitoes in the laboratory followed specific protocols. For the genetic variability analyses, the technique of horizontal electrophoresis on starch and starch-agarose gel with appropriate buffer systems was used. The alloenzyme variation was estimated using the Biosys-1 software. RESULTS: Out of the 13 loc
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Bria, Emilia Juliyanti, E. Suharyanto, and Purnomo Purnomo. "Variability and Intra-Specific Classification of Lima Bean (Phaseolus lunatus L.) from Timor Island based on Morphological Characters." Journal of Tropical Biodiversity and Biotechnology 4, no. 2 (2019): 62. http://dx.doi.org/10.22146/jtbb.42547.

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Lima bean (Phaseolus lunatus L.) is a species of beans which originating from the regions of Central America and Andes Mountains. Lima bean in Timor Island is underutilized although these plant growth there and have many variations. This study aims to determine the diversity of lima beans on Timor Island based on morphological characters. Samples were collected by survey methods from three districts on the island of Timor. Morphological traits related to the vegetative and flowering stages and mature seeds morphology were scored using the International Plant Genetic Resources Institute lima be
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Maghni, B., B. Hellal, and M. Maatoug. "Dynamics of the anatomical variability of Artemisia herba-alba in Algeria." Biosystems Diversity 26, no. 3 (2018): 239–44. http://dx.doi.org/10.15421/011836.

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In this work, the anatomical variability in Artemisia herba-alba Asso was studied based on 90 individuals taken from three different bioclimatic zones of Algeria (inferior semi arid, arid and Saharan) as well as the relationships of the dynamics of this variability with the distribution and adaptation of the species. The interpretation of the results of this study through the analysis of variance and the ascending hierarchical classification allowed us to detect a very high intra- and inter-specific anatomical variability, as well as the presence of a parenchyma of water reserves observed in i
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Rojas-Pérez, Víctor Eduardo, and Eduardo René Parra Villegas. "Inter-host Genetic Variability of Severe Acute Respiratory Syndrome Coronavirus 2 and its Implication in Biomedicine: A Bioinformatics Approach." Biomedical and Biotechnology Research Journal (BBRJ) 7, no. 4 (2023): 552–57. http://dx.doi.org/10.4103/bbrj.bbrj_256_23.

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Abstract Background: Genomic sequencing data from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have provided important information on the emergence of variants of interest. Some research has focused on determining intra-host variability and evolution, and others have focused on viral genetic variability in different tissues, as well as implications for virus infectivity and pathogenicity. However, the inter-host variability of SARS-CoV-2 in respiratory compartments and its implications has not yet been explained. To determine a possible pattern of tissue-specific inter-host var
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Barker, C., CJ Monaco, and CD McQuaid. "Exposure to fluctuating temperature increases thermal sensitivity in two lineages of the intertidal mussel Perna perna." Marine Ecology Progress Series 668 (June 24, 2021): 85–95. http://dx.doi.org/10.3354/meps13713.

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Studies of climate change effects on individuals and populations are mostly couched in terms of lethal temperature limits, but 2 other elements make predictions difficult: the effects of sublethal stress and intraspecific variability. Sublethal stress, such as exposure to highly variable temperatures, can cause carryover effects that influence how an animal responds to subsequent events and its long-term fitness. Additionally, intra-specific genetic variability can allow more complex species responses to climate change. We used 2 genetically distinct lineages of the intertidal mussel Perna per
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Noor, M., U. Nisar, and K. U. K. Muhhamad. "Genetic diversity in the natural population of threatened fruit- bearing plant species Monotheca buxifolia (Falc.) A.D." Ukrainian Journal of Ecology 10, no. 5 (2020): 44–48. http://dx.doi.org/10.15421/2020_204.

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Monotheca buxifolia is an ethnomedicinally and economically important threatened fruit bearing plant species in Malakand Division Pakistan. The genetic diversity among the 92 various genotypes of Monotheca buxifolia was carried out using sodium dodecyl sulfate poly acrylamide gel electrophoresis (SDS-PAGE) method. A considerable amount of inter districts genetic diversity (66.70%) was observed among the genotypes of M. buxifolia. Protein profiling was conducted on 12% gel electrophoresis. A total of 6 protein bands were observed in M. buxifolia genotypes. SDS-PAGE practice is a convenient sche
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Owens, K. W., F. A. Bliss, and C. E. Peterson. "Genetic Variation within and between Two Cucumber Populations Derived via the Inbred Backcross Line Method." Journal of the American Society for Horticultural Science 110, no. 3 (1985): 437–41. http://dx.doi.org/10.21273/jashs.110.3.437.

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Abstract Selected inbred backcross lines from 2 cucumber (Cucumis sativus L.) populations were studied to determine whether genetic variation existed within and between 2 populations for fruit length and weight. F1 hybrids from intra-population diallel and inter-population North Carolina Design II matings were evaluated in 1981 under greenhouse or field conditions. Significant genetic variability was found among selected inbred backcross lines within each population for fruit length and weight. General (GCA) and specific (SCA) combining ability estimates were significant, indicating that both
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Rossi, Franca, Carmela Amadoro, Maria Luigia Pallotta, and Giampaolo Colavita. "Variability of Genetic Characters Associated with Probiotic Functions in Lacticaseibacillus Species." Microorganisms 10, no. 5 (2022): 1023. http://dx.doi.org/10.3390/microorganisms10051023.

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This study aims to explore the intra-species distribution of genetic characteristics that favor the persistence in the gastrointestinal tract (GIT) and host interaction of bacteria belonging to species of the Lacticaseibacillus genus. These bacterial species comprise commercial probiotics with the widest use among consumers and strains naturally occurring in GIT and in fermented food. Since little is known about the distribution of genetic traits for adhesion capacity, polysaccharide production, biofilm formation, and utilization of substrates critically important for survival in GIT, which in
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Angelini, Claudio, Francesca Antonucci, Jacopo Aguzzi, and Corrado Costa. "Different Traits, Different Evolutionary Pathways: Insights from Salamandrina (Amphibia, Caudata)." Animals 12, no. 23 (2022): 3326. http://dx.doi.org/10.3390/ani12233326.

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Species delimitation is often based on a single or very few genetic or phenetic traits, something which leads to misinterpretations and often does not provide information about evolutionary processes. Here, we investigated the diversity pattern of multiple phenetic traits of the two extant species of Salamandrina, a genus split only after molecular traits had been studied but the two species of which are phenetically very similar. The phenetic traits we studied are size, external body shape and head colour pattern, in a model comparison framework using non-linear mixed models and unsupervised
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Giardini, María Cecilia, Fabián H. Milla, Silvia Lanzavecchia, Mariela Nieves, and Jorge L. Cladera. "Sex chromosomes in mitotic and polytene tissues of Anastrepha fraterculus (Diptera, Tephritidae) from Argentina: a review." ZooKeys 540 (November 26, 2015): 83–94. https://doi.org/10.3897/zookeys.540.6058.

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Cytogenetics, which is considered a fundamental tool to understand basic genetic and genomic issues of species, has greatly contributed to the description of polymorphisms both at inter- and intra-specific level. In fact, cytogenetics was one of the first approaches used to propose Anastrepha fraterculus (Diptera: Tephritidae) as a complex of cryptic species. Different morphological variants of sex chromosomes have been reported among Argentinean populations of A. fraterculus. However, since this high structural variability in sex chromosomes does not pose a reproductive barrier, their role in
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Xiao, Ming, Fubo Ma, Jun Yu, et al. "A Computer Simulation of SARS-CoV-2 Mutation Spectra for Empirical Data Characterization and Analysis." Biomolecules 13, no. 1 (2022): 63. http://dx.doi.org/10.3390/biom13010063.

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It is very important to compute the mutation spectra, and simulate the intra-host mutation processes by sequencing data, which is not only for the understanding of SARS-CoV-2 genetic mechanism, but also for epidemic prediction, vaccine, and drug design. However, the current intra-host mutation analysis algorithms are not only inaccurate, but also the simulation methods are unable to quickly and precisely predict new SARS-CoV-2 variants generated from the accumulation of mutations. Therefore, this study proposes a novel accurate strand-specific SARS-CoV-2 intra-host mutation spectra computation
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Tang, Jianming, John K. Moulton, Kenneth Pruess, Eddie W. Cupp, and Thomas R. Unnasch. "Genetic variation in North American black flies in the subgenus Psilopelmia (Simulium: Diptera: Simuliidae)." Canadian Journal of Zoology 76, no. 2 (1998): 205–11. http://dx.doi.org/10.1139/z97-190.

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Resolution of the genetic heterogeneity of closely related insect species depends on the selection of reliable genetic markers derived from representative specimens. We report the results of a survey of genetic variability in nine species of black flies in the subgenusPsilopelmia Enderlein. Three regions of the mitochondrial genome and an amplicon including the internal transcribed spacer 1 of the nuclear ribosomal RNA gene cluster (ITS1) were amplified using the polymerase chain reaction (PCR), and the amplicons were examined for intraspecific and interspecific polymorphisms. Six of the seven
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Dai, R. S., G. H. Liu, H. Q. Song, et al. "Sequence variability in two mitochondrial DNA regions and internal transcribed spacer among three cestodes infecting animals and humans from China." Journal of Helminthology 86, no. 2 (2011): 245–51. http://dx.doi.org/10.1017/s0022149x11000319.

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AbstractSequence variability in two mitochondrial DNA (mtDNA) regions, namely cytochromecoxidase subunit 1 (cox1) and NADH dehydrogenase subunit 4 (nad4), and internal transcribed spacer (ITS) of rDNA among and within three cestodes,Spirometra erinaceieuropaei,Taenia multicepsandTaenia hydatigena, from different geographical origins in China was examined. A portion of thecox1 (pcox1),nad4 genes (pnad4) and the ITS (ITS1+5.8S rDNA+ITS2) were amplified separately from individual cestodes by polymerase chain reaction (PCR). Representative amplicons were subjected to sequencing in order to estimat
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Shibana, C., and P. Subavathy. "DETERMINATION OF GENETIC DIVERSITY IN PORTUNUS PELAGICUS (LINNAEUS, 1758) COLLECTED FROM GULF OF MANNAR." International Journal of Advanced Research 9, no. 03 (2021): 409–20. http://dx.doi.org/10.21474/ijar01/12598.

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In the present study, the marine crab Portunuspelagicus was screened with 17 primers and made to score polymorphism. The main emphasis of the present study was to assess the genetic diversity at intra specific level among the 3 accessions of P. pelagicus species of Gulf of Mannarusing RAPD markers. RAPD analysis shows that there is a high level of polymorphism among different accessions. From this study, it was understood that each location varied with respect to environmental factors and genetic parameters. The OPB-18, OPB-19, OPC-07 and OPN-06 primers produced distinct, highly reproducible a
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Worth, James R. P., Luxian Liu, Fu-Jin Wei, and Nobuhiro Tomaru. "The complete chloroplast genome of Fagus crenata (subgenus Fagus) and comparison with F. engleriana (subgenus Engleriana)." PeerJ 7 (June 7, 2019): e7026. http://dx.doi.org/10.7717/peerj.7026.

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This study reports the whole chloroplast genome of Fagus crenata (subgenus Fagus), a foundation tree species of Japanese temperate forests. The genome has a total of 158,227 bp containing 111 genes, including 76 protein-coding genes, 31 tRNA genes and 4 ribosomal RNA genes. Comparison with the only other published Fagus chloroplast genome, F. engeleriana (subgenus Engleriana) shows that the genomes are relatively conserved with no inversions or rearrangements observed while the proportion of nucleotide sites differing between the two species was equal to 0.0018. The six most variable regions w
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Gerardin, Ylaine, Christian Kirkup, Archit Khosla, Laura Chambre, Michael Drage, and Amaro Taylor-Weiner. "Abstract PO1-15-01: Digital pathology models reveal case-specific characteristics of the tumor microenvironment." Cancer Research 84, no. 9_Supplement (2024): PO1–15–01—PO1–15–01. http://dx.doi.org/10.1158/1538-7445.sabcs23-po1-15-01.

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Abstract Background Phenotypic characteristics and genetic content have been observed to vary within individual tumors. However, translational research and some clinical practices often rely on extrapolating single histopathology slides to be representative of whole-tumor biology, even when the extent of intra-tumor biological heterogeneity is unknown. In addition, technical factors in slide preparation and digitization can also contribute to variability. Here, we use digital pathology models to identify cell and tissue types in whole-slide images of breast cancer tumors, and quantify intra- a
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Audebert, Chloe, Daphné Laubreton, Christophe Arpin, Olivier Gandrillon, Jacqueline Marvel, and Fabien Crauste. "Modeling and characterization of inter-individual variability in CD8 T cell responses in mice." In Silico Biology 14, no. 1-2 (2021): 13–39. http://dx.doi.org/10.3233/isb-200205.

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To develop vaccines it is mandatory yet challenging to account for inter-individual variability during immune responses. Even in laboratory mice, T cell responses of single individuals exhibit a high heterogeneity that may come from genetic backgrounds, intra-specific processes (e.g. antigen-processing and presentation) and immunization protocols. To account for inter-individual variability in CD8 T cell responses in mice, we propose a dynamical model coupled to a statistical, nonlinear mixed effects model. Average and individual dynamics during a CD8 T cell response are characterized in diffe
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47

Akter, Limu. "Genetic Divergence of Sweet Pepper in Bangladesh." Advances in Image and Video Processing 12, no. 6 (2024): 277–86. https://doi.org/10.14738/aivp.126.17951.

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The study of genetic divergence in sweet pepper is essential for enhancing crop yield, quality, and adaptability, especially in regions like Bangladesh where agriculture plays a critical role in the economy. The present investigation was carried out at the research farm of the Olericulture division, Horticulture Research Centre, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh to estimate genetic divergence in 21 sweet pepper genotypes based on fourteen characters using Mahalanobis’s D2 statistics during winter 2017-18 in randomized complete block design with three replications.
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Rueca, Martina, Barbara Bartolini, Cesare Ernesto Maria Gruber, et al. "Compartmentalized Replication of SARS-Cov-2 in Upper vs. Lower Respiratory Tract Assessed by Whole Genome Quasispecies Analysis." Microorganisms 8, no. 9 (2020): 1302. http://dx.doi.org/10.3390/microorganisms8091302.

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We report whole-genome and intra-host variability of SARS-Cov-2 assessed by next generation sequencing (NGS) in upper (URT) and lower respiratory tract (LRT) from COVID-19 patients. The aim was to identify possible tissue-specific patterns and signatures of variant selection for each respiratory compartment. Six patients, admitted to the Intensive Care Unit, were included in the study. Thirteen URT and LRT were analyzed by NGS amplicon-based approach on Ion Torrent Platform. Bioinformatic analysis was performed using both realized in-house and supplied by ThermoFisher programs. Phylogenesis sh
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MacLeod, Graham, Stephane Angers, Peter Dirks, et al. "GENE-31. IDENTIFICATION OF CORE AND CONTEXT-SPECIFIC FITNESS GENES IN GLIOBLASTOMA STEM CELLS VIA GENOME-WIDE CRISPR-Cas9 SCREENS." Neuro-Oncology 21, Supplement_6 (2019): vi104. http://dx.doi.org/10.1093/neuonc/noz175.433.

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Abstract Advances in the characterization of the genetic, epigenetic and transcriptomic landscapes of glioblastoma (GBM) tumor samples and primary cell lines have yielded considerable insight into tumor initiation and progression. A major finding from these studies is the high degree of inter- and intra-tumoral variability displayed in GBM, which has increased interest in the identification of clinically relevant disease subtypes for precision targeting. While multiple groups have identified subtypes of GBM tumors and GBM stem cell lines, efforts to identify specific actionable targets have ye
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MOLINA, Maria del Carmen, Pradeep K. DIVAKAR, Ana M. MILLANES, et al. "Parmelia sulcata (Ascomycota: Parmeliaceae), a sympatric monophyletic species complex." Lichenologist 43, no. 6 (2011): 585–601. http://dx.doi.org/10.1017/s0024282911000521.

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AbstractRecently, the number of cryptic species known has increased considerably, showing that species diversity has in many cases been underestimated in the past. Parmelia sulcata is a widely distributed species and one of the most common taxa in temperate Europe. The first intra-specific molecular studies on P. sulcata showed an unexpectedly high genetic variability. In the present work, we study the biodiversity of this taxon including specimens from four continents and using three molecular markers (nuITS, nuIGS rDNA, and partial β-tubulin gene). Two monophyletic groups of P. sulcata were
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