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Relevant bibliographies by topics / Genetic recombination. Recombinant proteins. Recombinant DNA / Journal articles

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Journal articles on the topic 'Genetic recombination. Recombinant proteins. Recombinant DNA'

Author: Grafiati

Published: 4 June 2021

Last updated: 13 February 2022

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1

Lloyd, R. G., and C. Buckman. "Conjugational recombination in Escherichia coli: genetic analysis of recombinant formation in Hfr x F- crosses." Genetics 139, no. 3 (1995): 1123–48. http://dx.doi.org/10.1093/genetics/139.3.1123.

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Abstract The formation of recombinants during conjugation between Hfr and F- strains of Escherichia coli was investigated using unselected markers to monitor integration of Hfr DNA into the circular recipient chromosome. In crosses selecting a marker located approximately 500 kb from the Hfr origin, 60-70% of the recombinants appeared to inherit the Hfr DNA in a single segment, with the proximal exchange located &gt; 300 kb from the selected marker. The proportion of recombinants showing multiple exchanges increased in matings selecting more distal markers located 700-2200 kb from the orig

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2

Osman, Fekret, Irina R. Tsaneva, Matthew C. Whitby, and Claudette L. Doe. "UV Irradiation Causes the Loss of Viable Mitotic Recombinants in Schizosaccharomyces pombe Cells Lacking the G2/M DNA Damage Checkpoint." Genetics 160, no. 3 (2002): 891–908. http://dx.doi.org/10.1093/genetics/160.3.891.

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Abstract Elevated mitotic recombination and cell cycle delays are two of the cellular responses to UV-induced DNA damage. Cell cycle delays in response to DNA damage are mediated via checkpoint proteins. Two distinct DNA damage checkpoints have been characterized in Schizosaccharomyces pombe: an intra-S-phase checkpoint slows replication and a G2/M checkpoint stops cells passing from G2 into mitosis. In this study we have sought to determine whether UV damage-induced mitotic intrachromosomal recombination relies on damage-induced cell cycle delays. The spontaneous and UV-induced recombination

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3

Losos, Jan K., David H. Evans, and Ann M. Verrinder Gibbins. "Targeted modification of the complete chicken lysozyme gene by poxvirus-mediated recombination." Biochemistry and Cell Biology 83, no. 2 (2005): 230–38. http://dx.doi.org/10.1139/o05-025.

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We have developed a novel ex vivo system for the rapid one-step targeted modification of large eucaryotic DNA sequences. The highly recombinant environment resulting from infection of rabbit cornea cells with the Shope fibroma virus was exploited to mediate precise modifications of the complete chicken lysozyme gene domain (21.5 kb). Homologous recombination was designed to occur between target DNA (containing the complete lysozyme gene domain) maintained in a λ bacteriophage vector and modified targeting DNA maintained in a plasmid. The targeting plasmids were designed to transfer exogenous s

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4

Lefeuvre, P., J. M. Lett, A. Varsani, and D. P. Martin. "Widely Conserved Recombination Patterns among Single-Stranded DNA Viruses." Journal of Virology 83, no. 6 (2008): 2697–707. http://dx.doi.org/10.1128/jvi.02152-08.

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ABSTRACT The combinatorial nature of genetic recombination can potentially provide organisms with immediate access to many more positions in sequence space than can be reached by mutation alone. Recombination features particularly prominently in the evolution of a diverse range of viruses. Despite rapid progress having been made in the characterization of discrete recombination events for many species, little is currently known about either gross patterns of recombination across related virus families or the underlying processes that determine genome-wide recombination breakpoint distributions

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5

Durmaz, Evelyn, and Todd R. Klaenhammer. "Genetic Analysis of Chromosomal Regions ofLactococcus lactis Acquired by Recombinant Lytic Phages." Applied and Environmental Microbiology 66, no. 3 (2000): 895–903. http://dx.doi.org/10.1128/aem.66.3.895-903.2000.

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ABSTRACT Recombinant phages are generated when Lactococcus lactis subsp. lactis harboring plasmids encoding the abortive type (Abi) of phage resistance mechanisms is infected with small isometric phages belonging to the P335 species. These phage variants are likely to be an important source of virulent new phages that appear in dairy fermentations. They are distinguished from their progenitors by resistance to Abi defenses and by altered genome organization, including regions of L. lactis chromosomal DNA. The objective of this study was to characterize four recombinant variants that arose from

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6

Garcia-Ruiz, Hernan, and Paul Ahlquist. "Inducible Yeast System for Viral RNA Recombination Reveals Requirement for an RNA Replication Signal on Both Parental RNAs." Journal of Virology 80, no. 17 (2006): 8316–28. http://dx.doi.org/10.1128/jvi.01790-05.

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ABSTRACT To facilitate RNA recombination studies, we tested whether Saccharomyces cerevisiae, which supports brome mosaic virus (BMV) replication, also supports BMV RNA recombination. Yeast strains expressing BMV RNA replication proteins 1a and 2apol were engineered to transiently coexpress two independently inducible, overlapping, nonreplicating derivatives of BMV genomic RNA3. B3Δ3′ lacked the coat protein gene and negative-strand RNA promoter. B3Δ5′ lacked the positive-strand RNA promoter and had the coat gene replaced by the selectable URA3 gene. After 12 to 72 h of induction, B3Δ3′ and B3

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7

Sun, Qing, Nicholas C. Collins, Michael Ayliffe, et al. "Recombination Between Paralogues at the rp1 Rust Resistance Locus in Maize." Genetics 158, no. 1 (2001): 423–38. http://dx.doi.org/10.1093/genetics/158.1.423.

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Abstract Rp1 is a complex rust resistance locus of maize. The HRp1-D haplotype is composed of Rp1-D and eight paralogues, seven of which also code for predicted nucleotide binding site-leucine rich repeat (NBS-LRR) proteins similar to the Rp1-D gene. The paralogues are polymorphic (DNA identities 91-97%), especially in the C-terminal LRR domain. The remaining family member encodes a truncated protein that has no LRR domain. Seven of the nine family members, including the truncated gene, are transcribed. Sequence comparisons between paralogues provide evidence for past recombination events betw

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8

Saintigny, Yannick, Kate Makienko, Cristina Swanson, Mary J. Emond, and Raymond J. Monnat,. "Homologous Recombination Resolution Defect in Werner Syndrome." Molecular and Cellular Biology 22, no. 20 (2002): 6971–78. http://dx.doi.org/10.1128/mcb.22.20.6971-6978.2002.

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ABSTRACT Werner syndrome (WRN) is an uncommon autosomal recessive disease whose phenotype includes features of premature aging, genetic instability, and an elevated risk of cancer. We used three different experimental strategies to show that WRN cellular phenotypes of limited cell division potential, DNA damage hypersensitivity, and defective homologous recombination (HR) are interrelated. WRN cell survival and the generation of viable mitotic recombinant progeny could be rescued by expressing wild-type WRN protein or by expressing the bacterial resolvase protein RusA. The dependence of WRN ce

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9

Li, Ting, and Jiayou Zhang. "Determination of the Frequency of Retroviral Recombination between Two Identical Sequences within a Provirus." Journal of Virology 74, no. 16 (2000): 7646–50. http://dx.doi.org/10.1128/jvi.74.16.7646-7650.2000.

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ABSTRACT Retroviruses use RNA as their genetic material within viral particles and DNA (provirus) as their genetic material within cells. The rate of recombination during reverse transcription between two identical sequences within the same RNA molecule is very high. In this study, we have developed a sensitive system to study recombination occurring within the proviral sequence. This system includes a murine Moloney leukemia virus vector which contains a neomycin resistance gene (neo) and two mutated green fluorescent protein genes (gfp) in tandem positions. The 3′ end of the firstgfp and the

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10

Beisenov, D. K., G. E. Stanbekova та B. K. Iskakov. "Тransplastomic tobacco plants producing the hydrophilic domain of the sheep pox virus coat protein L1R". Vavilov Journal of Genetics and Breeding 24, № 8 (2020): 905–12. http://dx.doi.org/10.18699/vj20.689.

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Sheep pox has a wide geographical range of distribution and poses a threat to sheep breeding worldwide, as the disease is highly contagious and is accompanied by large economic losses. Vaccines based on live attenuated virus strains are currently being used for prevention of this disease. Such vaccines are effective, but potentially dangerous because of the possible virus reversion to a pathogenic state. The development of safe recombinant subunit vaccines against sheep pox is very relevant. The high ploidy level of the plant chloroplasts makes it possible to obtain large quantities of foreign

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11

Appasani, Krishnarao, David S. Thaler, and Edward B. Goldberg. "Bacteriophage T4 gp2 Interferes with Cell Viability and with Bacteriophage Lambda Red Recombination." Journal of Bacteriology 181, no. 4 (1999): 1352–55. http://dx.doi.org/10.1128/jb.181.4.1352-1355.1999.

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ABSTRACT The T4 head protein, gp2, promotes head-tail joining during phage morphogenesis and is also incorporated into the phage head. It protects the injected DNA from degradation by exonuclease V during the subsequent infection. In this study, we show that recombinant gp2, a very basic protein, rapidly kills the cells in which it is expressed. To further illustrate the protectiveness of gp2 for DNA termini, we compare the effect of gp2 expression on Red-mediated and Int-mediated recombination. Red-mediated recombination is nonspecific and requires the transient formation of double-stranded D

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12

Kitten, T., and A. G. Barbour. "The relapsing fever agent Borrelia hermsii has multiple copies of its chromosome and linear plasmids." Genetics 132, no. 2 (1992): 311–24. http://dx.doi.org/10.1093/genetics/132.2.311.

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Abstract Borrelia hermsii, a spirochete which causes relapsing fever in humans and other mammals, eludes the immune response by antigenic variation of the "Vmp" proteins. This occurs by replacement of an expressed vmp gene with a copy of a silent vmp gene. Silent and expressed vmp genes are located on separate linear plasmids. To further characterize vmp recombination, copy numbers were determined for two linear plasmids and for the 1-megabase chromosome by comparing hybridization of probes to native DNA with hybridization to recombinant plasmids containing borrelial DNA. Plasmid copy numbers

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13

Crouch, Erin A., and A. Lorena Passarelli. "Genetic Requirements for Homologous Recombination in Autographa californica Nucleopolyhedrovirus." Journal of Virology 76, no. 18 (2002): 9323–34. http://dx.doi.org/10.1128/jvi.76.18.9323-9334.2002.

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ABSTRACT It is known that baculovirus infection promotes high-frequency recombination between its genomes and plasmid DNA during the construction of recombinant viruses for foreign gene expression. However, little is known about the viral genes necessary to promote homologous recombination (HR). We developed an assay to identify viral genes that are necessary to stimulate HR. In this assay, we used two plasmids containing extensive sequence homology that yielded a visible and quantifiable phenotype if HR occurred. The plasmids contained the green fluorescent protein gene (gfp) that was mutated

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14

Lin, Y. C. James, and D. H. Evans. "Vaccinia Virus Particles Mix Inefficiently, and in a Way That Would Restrict Viral Recombination, in Coinfected Cells." Journal of Virology 84, no. 5 (2009): 2432–43. http://dx.doi.org/10.1128/jvi.01998-09.

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ABSTRACT It is well established that poxviruses are subjected to genetic recombination, but attempts to map vaccinia virus genes using classical genetic crosses were historically confounded by high levels of experimental noise and a poor correlation between physical and genetic map distances. These virus-by-virus crosses also never produced the 50% recombinant progeny that should be seen in experiments involving distant markers. Poxviruses replicate in membrane-wrapped cytoplasmic structures called virosomes (or factories) and we have developed a method for tracking the development of these st

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15

Merrihew, Raymond V., William C. Clay, J. Patrick Condreay, Sam M. Witherspoon, Walter S. Dallas, and Thomas A. Kost. "Chromosomal Integration of Transduced Recombinant Baculovirus DNA in Mammalian Cells." Journal of Virology 75, no. 2 (2001): 903–9. http://dx.doi.org/10.1128/jvi.75.2.903-909.2001.

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ABSTRACT Our group and others have recently demonstrated the ability of recombinant baculoviruses to transduce mammalian cells at high frequency. To further characterize the use of baculovirus as a mammalian gene delivery system, we examined the status of transduced DNA stably maintained in Chinese hamster ovary (CHO) cells. Four independent clones carrying two introduced markers, the genes for neomycin resistance (Neo) and green fluorescent protein (GFP), were selected. PCR analysis, Southern blotting, and DNA sequencing showed that discrete portions of the 148-kb baculovirus DNA were present

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16

Gratias, Ariane, and Valérie Geffroy. "Deciphering the Impact of a Bacterial Infection on Meiotic Recombination in Arabidopsis with Fluorescence Tagged Lines." Genes 11, no. 7 (2020): 832. http://dx.doi.org/10.3390/genes11070832.

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Plants are under strong evolutionary pressure to maintain surveillance against pathogens. One major disease resistance mechanism is based on NB-LRR (NLR) proteins that specifically recognize pathogen effectors. The cluster organization of the NLR gene family could favor sequence exchange between NLR genes via recombination, favoring their evolutionary dynamics. Increasing data, based on progeny analysis, suggest the existence of a link between the perception of biotic stress and the production of genetic diversity in the offspring. This could be driven by an increased rate of meiotic recombina

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17

Wu, Yuliang, Joshua A. Sommers, Avvaru N. Suhasini, et al. "Fanconi anemia group J mutation abolishes its DNA repair function by uncoupling DNA translocation from helicase activity or disruption of protein-DNA complexes." Blood 116, no. 19 (2010): 3780–91. http://dx.doi.org/10.1182/blood-2009-11-256016.

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Abstract Fanconi anemia (FA) is a genetic disease characterized by congenital abnormalities, bone marrow failure, and susceptibility to leukemia and other cancers. FANCJ, one of 13 genes linked to FA, encodes a DNA helicase proposed to operate in homologous recombination repair and replicational stress response. The pathogenic FANCJ-A349P amino acid substitution resides immediately adjacent to a highly conserved cysteine of the iron-sulfur domain. Given the genetic linkage of the FANCJ-A349P allele to FA, we investigated the effect of this particular mutation on the biochemical and cellular fu

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18

Werbowy, Olesia, Aleksandra Stefańska-Kaźmierczak, Agata Jurczak-Kurek, et al. "The Characteristics of New SSB Proteins from Metagenomic Libraries and Their Use in Biotech Applications." Proceedings 50, no. 1 (2020): 135. http://dx.doi.org/10.3390/proceedings2020050135.

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Single-stranded DNA binding proteins (SSBs) bind to single-stranded DNA in a sequence-independent manner to prevent formation of secondary structures and protect DNA from nuclease degradation. These ubiquitous proteins are present in prokaryotes, eukaryotes, and viruses, and play a pivotal role in the following major cellular processes: replication, recombination, and repair of genetic material. In DNA replication, SSB proteins specifically stimulate DNA polymerase, increase fidelity of DNA synthesis, assist the advance of DNA polymerase, and organize and stabilize replication forks. Here, we

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19

Chang, Hao-Yen, Chia-Yi Lee, Chih-Hao Lu, et al. "Microcephaly family protein MCPH1 stabilizes RAD51 filaments." Nucleic Acids Research 48, no. 16 (2020): 9135–46. http://dx.doi.org/10.1093/nar/gkaa636.

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Abstract Microcephalin 1 (MCPH1) was identified from genetic mutations in patients with primary autosomal recessive microcephaly. In response to DNA double-strand breaks (DSBs), MCPH1 forms damage-induced foci and recruits BRCA2–RAD51 complex, a key component of the DSB repair machinery for homologous recombination (HR), to damage sites. Accordingly, the efficiency of HR is significantly attenuated upon depletion of MCPH1. The biochemical characteristics of MCPH1 and its functional interaction with the HR machinery had remained unclear due to lack of highly purified MCPH1 recombinant protein f

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Hallett, Stephen T., Pascale Schellenberger, Lihong Zhou, et al. "Nse5/6 is a negative regulator of the ATPase activity of the Smc5/6 complex." Nucleic Acids Research 49, no. 8 (2021): 4534–49. http://dx.doi.org/10.1093/nar/gkab234.

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Abstract The multi-component Smc5/6 complex plays a critical role in the resolution of recombination intermediates formed during mitosis and meiosis, and in the cellular response to replication stress. Using recombinant proteins, we have reconstituted a series of defined Saccharomyces cerevisiae Smc5/6 complexes, visualised them by negative stain electron microscopy, and tested their ability to function as an ATPase. We find that only the six protein ‘holo-complex’ is capable of turning over ATP and that its activity is significantly increased by the addition of double-stranded DNA to reaction

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21

Ainger, K., E. Barbarese, L. Berman, and J. H. Carson. "Molecular genetic analysis of the mldr mouse: a spontaneous revertant at the mld locus containing a recombinant myelin basic protein gene." Genetics 130, no. 2 (1992): 367–75. http://dx.doi.org/10.1093/genetics/130.2.367.

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Abstract The mld mutation is a complex genetic lesion affecting the myelin basic protein (MBP) locus in the mouse. The mutation consists of a variety of DNA rearrangements including: tandem duplication of the MBP structural gene, partial inversion of the 3' end of the upstream gene copy, duplication of a region flanking the rearrangement junction in the upstream copy and insertion between the two gene copies of a segment of extraneous DNA not associated with the wild-type MBP locus. The net result of the mutation is a dysfunctional MBP locus. Homozygous mld/mld mice produce very little MBP and

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22

Koloskova, E. M., V. A. Ezerskiy, and K. S. Ostrenko. "Designing of a DNA matrix for transgene integration into the bovine beta-lactoglobulin gene locus using CRISPR/Cas9 technology." Ukrainian Journal of Ecology 10, no. 5 (2020): 206–10. http://dx.doi.org/10.15421/2020_231.

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Beta-lactoglobulin (BLG) is the main protein in milk serum in almost all mammals, with the exception of rodents and primates. Regulatory regions of the beta-lactoglobulin gene in ruminants (sheep, goats, and cattle) as part of genetic constructs provide tissue - specific expression of recombinant protein in the mammary gland and have been actively used in genetic engineering since the beginning of the era of creating transgenic animals. To work effectively with the CRISPR/Cas9 genomic editing method, it is necessary to know the exact DNA sequence of the target gene: this is necessary both for

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23

Motomura, Kazushi, Jianbo Chen, and Wei-Shau Hu. "Genetic Recombination between Human Immunodeficiency Virus Type 1 (HIV-1) and HIV-2, Two Distinct Human Lentiviruses." Journal of Virology 82, no. 4 (2007): 1923–33. http://dx.doi.org/10.1128/jvi.01937-07.

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ABSTRACT Human immunodeficiency virus type 1 (HIV-1) and HIV-2 are genetically distinct viruses that each can cause AIDS. Approximately 1 million people are infected with both HIV-1 and HIV-2. Additionally, these two viruses use the same receptor and coreceptors and can therefore infect the same target cell populations. To explore potential genetic interactions, we first examined whether RNAs from HIV-1 and HIV-2 can be copackaged into the same virion. We used modified near-full-length viruses that each contained a green fluorescent protein gene (gfp) with a different inactivating mutation. Th

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Liu, Xiaoming, Ziying Yan, Meihui Luo, et al. "Targeted Correction of Single-Base-Pair Mutations with Adeno-Associated Virus Vectors under Nonselective Conditions." Journal of Virology 78, no. 8 (2004): 4165–75. http://dx.doi.org/10.1128/jvi.78.8.4165-4175.2004.

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ABSTRACT Recombinant adeno-associated virus (rAAV) vectors possess the unique ability to introduce genetic alterations at sites of homology in genomic DNA through a mechanism thought to predominantly involve homologous recombination. We have investigated the efficiency of this approach using a mutant enhanced green fluorescent protein (eGFP) fluorescence recovery assay that facilitates detection of gene correction events in living cells under nonselective conditions. Our data demonstrate that rAAV infection can correct a mutant eGFP transgene at an efficiency of 0.1% in 293 cells, as determine

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25

Štambuk, Snježana, and Miroslav Radman. "Mechanism and Control of Interspecies Recombination in Escherichia coli. I. Mismatch Repair, Methylation, Recombination and Replication Functions." Genetics 150, no. 2 (1998): 533–42. http://dx.doi.org/10.1093/genetics/150.2.533.

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Abstract A genetic analysis of interspecies recombination in Escherichia coli between the linear Hfr DNA from Salmonella typhimurium and the circular recipient chromosome reveals some fundamental aspects of recombination between related DNA sequences. The MutS and MutL mismatch binding proteins edit (prevent) homeologous recombination between these 16% diverged genomes by at least two distinct mechanisms. One is MutH independent and presumably acts by aborting the initiated recombination through the UvrD helicase activity. The RecBCD nuclease might contribute to this editing step, presumably b

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Nanassy, Oliver Z., and Kelly T. Hughes. "Hin Recombinase Mutants Functionally Disrupted in Interactions with Fis." Journal of Bacteriology 183, no. 1 (2001): 28–35. http://dx.doi.org/10.1128/jb.183.1.28-35.2001.

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ABSTRACT A previous genetic screen was designed to separate Hin recombinase mutants into distinct classes based on the stage in the recombination reaction at which they are blocked (O. Nanassy, Zoltan, and K. T. Hughes, Genetics 149:1649–1663, 1998). One class of DNA binding-proficient, recombination-deficient mutants was predicted by genetic classification to be defective in the step prior to invertasome formation. Based on the genetic criteria, mutants from this class were also inferred to be defective in interactions with Fis. In order to understand how the genetic classification relates to

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27

Nanassy, Oliver Z., and Kelly T. Hughes. "In Vivo Identification of Intermediate Stages of the DNA Inversion Reaction Catalyzed by the Salmonella Hin Recombinase." Genetics 149, no. 4 (1998): 1649–63. http://dx.doi.org/10.1093/genetics/149.4.1649.

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Abstract The Hin recombinase catalyzes a site-specific recombination reaction that results in the reversible inversion of a 1-kbp segment of the Salmonella chromosome. The DNA inversion reaction catalyzed by the Salmonella Hin recombinase is a dynamic process proceeding through many intermediate stages, requiring multiple DNA sites and the Fis accessory protein. Biochemical analysis of this reaction has identified intermediate steps in the inversion reaction but has not yet revealed the process by which transition from one step to another occurs. Because transition from one reaction step to an

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28

Bevacqua, R. J., D. Carlson, R. Fernandez-Martín та ін. "199 Efficient Knock-out of Ovine β-Lactoglobulin (BLG) Gene and Knock-in of Recombinant Human Factor IX (rhFIX) Under BLG Native Regulatory Sequences in Somatic Cells and Zygotes Using TALEN Nuclease". Reproduction, Fertility and Development 30, № 1 (2018): 240. http://dx.doi.org/10.1071/rdv30n1ab199.

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Site-specific genetic engineering is a valuable tool for pharmaceutical research and development of biomedical models. Despite engineered nucleases allow targeted gene edition in a rather simple fashion; few reports are available so far on specific gene knock-in (KI) combined with engineered nucleases in domestic species. Here, we evaluated the possibility of inducing specific KI of cDNAs coding for proteins of pharmaceutical interest under the control of milk native promoter sequences, taking advantage of the TALEN system, both in ovine somatic cells and in zygotes. We designed 2 TALENs, targ

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29

Matic, I., M. Radman, and C. Rayssiguier. "Structure of recombinants from conjugational crosses between Escherichia coli donor and mismatch-repair deficient Salmonella typhimurium recipients." Genetics 136, no. 1 (1994): 17–26. http://dx.doi.org/10.1093/genetics/136.1.17.

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Abstract To get more insight into the control of homologous recombination between diverged DNA by the Mut proteins of the long-patch mismatch repair system, we have studied interspecies Escherichia coli/Salmonella typhimurium recombination. Knowing that the same recombination pathway (RecABCD) is responsible for intraspecies and interspecies recombination, we have now studied the structure (replacement vs. addition-type or other rearrangement-type recombinants) of 81 interspecies recombinants obtained in conjugational crosses between E. coli donor and mutL, mutS, mutH, mutU or mut+ S. typhimur

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Karikari, Benjamin, Shuguang Li, Javaid Bhat, et al. "Genome-Wide Detection of Major and Epistatic Effect QTLs for Seed Protein and Oil Content in Soybean Under Multiple Environments Using High-Density Bin Map." International Journal of Molecular Sciences 20, no. 4 (2019): 979. http://dx.doi.org/10.3390/ijms20040979.

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Seed protein and oil content are the two important traits determining the quality and value of soybean. Development of improved cultivars requires detailed understanding of the genetic basis underlying the trait of interest. However, it is prerequisite to have a high-density linkage map for precisely mapping genomic regions, and therefore the present study used high-density genetic map containing 2267 recombination bin markers distributed on 20 chromosomes and spanned 2453.79 cM with an average distance of 1.08 cM between markers using restriction-site-associated DNA sequencing (RAD-seq) appro

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Lebreton, B., P. V. Prasad, M. Jayaram, and P. Youderian. "Mutations that improve the binding of yeast FLP recombinase to its substrate." Genetics 118, no. 3 (1988): 393–400. http://dx.doi.org/10.1093/genetics/118.3.393.

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Abstract When yeast FLP recombinase is expressed from the phage lambda PR promoter in a Salmonella host, it cannot efficiently repress an operon controlled by an operator/promoter region that includes a synthetic, target FLP site. On the basis of this phenotype, we have identified four mutant FLP proteins that function as more efficient repressors of such an operon. At least two of these mutant FLP proteins bind better to the FLP site in vivo and in vitro. One mutant changes the presumed active site tyrosine residue of FLP protein to phenylalanine, is blocked in recombination, and binds the FL

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Moens, Peter B. "The double-stranded DNA helix in recombination at meiosis." Genome 46, no. 6 (2003): 936–37. http://dx.doi.org/10.1139/g03-111.

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The nature of meiotic genetic recombination was resolved at the DNA level by the 1953 Watson and Crick model. What remains to be determined are the roles of the various recombination proteins and the distribution and localization of recombination events in the meiotic prophase nucleus.Key words: Holliday model, Holliday junction, chromosome cores, meiosis, recombinase, checkpoints, gene conversion, cross over.

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Chittela, Rajani Kant, and Jayashree K. Sainis. "Plant DNA Recombinases: A Long Way to Go." Journal of Nucleic Acids 2010 (2010): 1–10. http://dx.doi.org/10.4061/2010/646109.

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DNA homologous recombination is fundamental process by which two homologous DNA molecules exchange the genetic information for the generation of genetic diversity and maintain the genomic integrity. DNA recombinases, a special group of proteins bind to single stranded DNA (ssDNA) nonspecifically and search the double stranded DNA (dsDNA) molecule for a stretch of DNA that is homologous with the bound ssDNA. Recombinase A (RecA) has been well characterized at genetic, biochemical, as well as structural level from prokaryotes. Two homologues of RecA called Rad51 and Dmc1 have been detected in ye

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34

Strathern, J. N., K. G. Weinstock, D. R. Higgins, and C. B. McGill. "A novel recombinator in yeast based on gene II protein from bacteriophage f1." Genetics 127, no. 1 (1991): 61–73. http://dx.doi.org/10.1093/genetics/127.1.61.

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Abstract Interchromosomal mitotic recombination in yeast can be stimulated by the protein encoded by gene II of bacteriophage f1. The normal role of the gene II enzyme is to make a site-specific cleavage of a particular strand of the duplex form of the bacteriophage DNA at the origin of DNA replication. The gene II protein was expressed in yeast in an attempt to determine the role of nicked DNA in the initiation of recombination. Stimulation of recombination in yeast by the gene II protein was dependent on the presence of a recognition site for gene II enzyme in the region being assayed. Recom

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35

Tishkoff, D. X., B. Rockmill, G. S. Roeder, and R. D. Kolodner. "The sep1 mutant of Saccharomyces cerevisiae arrests in pachytene and is deficient in meiotic recombination." Genetics 139, no. 2 (1995): 495–509. http://dx.doi.org/10.1093/genetics/139.2.495.

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Abstract Strand exchange protein 1 (Sep1) from Saccharomyces cerevisiae promotes homologous pairing of DNA in vitro and sep1 mutants display pleiotropic phenotypes in both vegetative and meiotic cells. In this study, we examined in detail the ability of the sep1 mutant to progress through meiosis I prophase and to undergo meiotic recombination. In meiotic return-to-growth experiments, commitment to meiotic recombination began at the same time in wild type and mutant; however, recombinants accumulated at decreased rates in the mutant. Gene conversion eventually reached nearly wild-type levels,

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Shinohara, Miki, Emi Shita-Yamaguchi, Jean-Marie Buerstedde, Hideo Shinagawa, Hideyuki Ogawa, and Akira Shinohara. "Characterization of the Roles of the Saccharomyces cerevisiae RAD54 Gene and a Homologue of RAD54, RDH54/TID1, in Mitosis and Meiosis." Genetics 147, no. 4 (1997): 1545–56. http://dx.doi.org/10.1093/genetics/147.4.1545.

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Abstract The RAD54 gene, which encodes a protein in the SW12/SNF2 family, plays an important role in recombination and DNA repair in Saccharomyces cerevisiae. The yeast genome project revealed a homologue of RAD54, RDH54/TID1. Properties of the rdh54/tid1 mutant and the rad54 rdh54/tid1 double mutant are shown for mitosis and meiosis. The rad54 mutant is sensitive to the alkylating agent, methyl methanesulfonate (MMS), and is defective in interchromosomal and intrachromosomal gene conversion. The rdh54/tid1 single mutant, on the other hand, does not show any significant deficiency in mitosis.

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Yokochi, T., K. Kusano, and I. Kobayashi. "Evidence for conservative (two-progeny) DNA double-strand break repair." Genetics 139, no. 1 (1995): 5–17. http://dx.doi.org/10.1093/genetics/139.1.5.

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Abstract The double-strand break repair models for homologous recombination propose that a double-strand break in a duplex DNA segment is repaired by gene conversion copying a homologous DNA segment. This is a type of conservative recombination, or two-progeny recombination, which generates two duplex DNA segments from two duplex DNA segments. Transformation with a plasmid carrying a double-strand gap and an intact homologous DNA segment resulted in products expected from such conservative (two-progeny) repair in Escherichia coli cells with active E. coli RecE pathway (recBC sbcA) or with acti

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Burgess, Rebecca C., Michael Lisby, Veronika Altmannova, Lumir Krejci, Patrick Sung, and Rodney Rothstein. "Localization of recombination proteins and Srs2 reveals anti-recombinase function in vivo." Journal of Cell Biology 185, no. 6 (2009): 969–81. http://dx.doi.org/10.1083/jcb.200810055.

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Homologous recombination (HR), although an important DNA repair mechanism, is dangerous to the cell if improperly regulated. The Srs2 “anti-recombinase” restricts HR by disassembling the Rad51 nucleoprotein filament, an intermediate preceding the exchange of homologous DNA strands. Here, we cytologically characterize Srs2 function in vivo and describe a novel mechanism for regulating the initiation of HR. We find that Srs2 is recruited separately to replication and repair centers and identify the genetic requirements for recruitment. In the absence of Srs2 activity, Rad51 foci accumulate, and

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Candelli, Andrea, Mauro Modesti, Erwin J. G. Peterman, and Gijs J. L. Wuite. "Single-molecule views on homologous recombination." Quarterly Reviews of Biophysics 46, no. 4 (2013): 323–48. http://dx.doi.org/10.1017/s0033583513000073.

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AbstractAll organisms need homologous recombination (HR) to repair DNA double-strand breaks. Defects in recombination are linked to genetic instability and to elevated risks in developing cancers. The central catalyst of HR is a nucleoprotein filament, consisting of recombinase proteins (human RAD51 or bacterial RecA) bound around single-stranded DNA. Over the last two decades, single-molecule techniques have provided substantial new insights into the dynamics of homologous recombination. Here, we survey important recent developments in this field of research and provide an outlook on future d

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Shirakata, M., K. Hüppi, S. Usuda, K. Okazaki, K. Yoshida, and H. Sakano. "HMG1-related DNA-binding protein isolated with V-(D)-J recombination signal probes." Molecular and Cellular Biology 11, no. 9 (1991): 4528–36. http://dx.doi.org/10.1128/mcb.11.9.4528.

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In order to isolate cDNA clones for DNA-binding components of the V-(D)-J recombinase, phage libraries from a pre-B-cell line were screened with a radiolabeled probe containing recombination signal sequences (RSS). Among prospective clones, cDNA T160 was analyzed further. It produced a protein of 80.6 kDa which bound to DNA containing RSS but not to DNA in which the RSS had been mutated. A search of a data base revealed that the T160 protein has significant sequence homology (56%) to the nonhistone chromosomal protein HMG1 within the C-terminal region of 80 amino acids. DNA-binding analysis wi

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Shirakata, M., K. Hüppi, S. Usuda, K. Okazaki, K. Yoshida, and H. Sakano. "HMG1-related DNA-binding protein isolated with V-(D)-J recombination signal probes." Molecular and Cellular Biology 11, no. 9 (1991): 4528–36. http://dx.doi.org/10.1128/mcb.11.9.4528-4536.1991.

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In order to isolate cDNA clones for DNA-binding components of the V-(D)-J recombinase, phage libraries from a pre-B-cell line were screened with a radiolabeled probe containing recombination signal sequences (RSS). Among prospective clones, cDNA T160 was analyzed further. It produced a protein of 80.6 kDa which bound to DNA containing RSS but not to DNA in which the RSS had been mutated. A search of a data base revealed that the T160 protein has significant sequence homology (56%) to the nonhistone chromosomal protein HMG1 within the C-terminal region of 80 amino acids. DNA-binding analysis wi

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Mortensen, Uffe H., Naz Erdeniz, Qi Feng, and Rodney Rothstein. "A Molecular Genetic Dissection of the Evolutionarily Conserved N Terminus of Yeast Rad52." Genetics 161, no. 2 (2002): 549–62. http://dx.doi.org/10.1093/genetics/161.2.549.

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Abstract Rad52 is a DNA-binding protein that stimulates the annealing of complementary single-stranded DNA. Only the N terminus of Rad52 is evolutionarily conserved; it contains the core activity of the protein, including its DNA-binding activity. To identify amino acid residues that are important for Rad52 function(s), we systematically replaced 76 of 165 amino acid residues in the N terminus with alanine. These substitutions were examined for their effects on the repair of γ-ray-induced DNA damage and on both interchromosomal and direct repeat heteroallelic recombination. This analysis ident

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Hogrel, Gaëlle, Yang Lu, Nicolas Alexandre, et al. "Role of RadA and DNA Polymerases in Recombination-Associated DNA Synthesis in Hyperthermophilic Archaea." Biomolecules 10, no. 7 (2020): 1045. http://dx.doi.org/10.3390/biom10071045.

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Among the three domains of life, the process of homologous recombination (HR) plays a central role in the repair of double-strand DNA breaks and the restart of stalled replication forks. Curiously, main protein actors involved in the HR process appear to be essential for hyperthermophilic Archaea raising interesting questions about the role of HR in replication and repair strategies of those Archaea living in extreme conditions. One key actor of this process is the recombinase RadA, which allows the homologous strand search and provides a DNA substrate required for following DNA synthesis and

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Poteete, A. R., and A. C. Fenton. "Efficient double-strand break-stimulated recombination promoted by the general recombination systems of phages lambda and P22." Genetics 134, no. 4 (1993): 1013–21. http://dx.doi.org/10.1093/genetics/134.4.1013.

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Abstract To examine bacteriophage recombination in vivo, independent of such other processes as replication and packaging, substituted lambda phages bearing restriction site polymorphisms were employed in a direct physical assay. Bacteria were infected with two phage variants; DNA was extracted from the infected cells and cut with a restriction endonuclease. The production of a unique recombinant fragment was measured by Southern blotting and hybridization with a substitution sequence-specific probe. High frequency recombination was observed under the following conditions: the substituted lamb

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Zhou, Wenhua, and Han-Mou Tsai. "The Carboxyl-Terminal Domains of ADAMTS13 Cause Substrate-Dependent Divergence of ADAMTS13 Activity." Blood 108, no. 11 (2006): 381. http://dx.doi.org/10.1182/blood.v108.11.381.381.

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ADAMTS13, a circulating metalloprotease that cleaves the Y1605-M1606 bond of VWF, is critical for preventing intravascular platelet thrombosis of thrombotic thrombocytopenic purpura. Unlike genetic deficiency of ADAMTS13 in patients, inactivation of ADAMTS13 created by homologous recombination causes either no or delayed-onset phenotypic abnormalities in mice, depending on the strains examined. In order to further understand the role of ADAMTS13 in VWF homeostasis, we investigated the structure and function of the enzyme in various mouse strains. As in human subjects, RT PCR analysis revealed

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Kumagai, M., T. Yamashita, M. Honda, and H. Ikeda. "Effects of uvsX, uvsY and DNA topoisomerase on the formation of tandem duplications of the rII gene in bacteriophage T4." Genetics 135, no. 2 (1993): 255–64. http://dx.doi.org/10.1093/genetics/135.2.255.

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Abstract We have characterized tandem duplications in the rII regions of phage T4. The rII deletion r1589 blocks only the function of the rIIA cistron, although it extends into the B cistron. Another rII deletion, r1236, blocks the function of the rIIB cistron and overlaps r1589. When a cross is made between r1589 and r1236, true rII+ progeny cannot form. Instead, anomalous phenotypically rII+ phages are detected carrying an rII region from each parent. Analyses of nucleotide sequences of the recombination junctions indicate that recombination takes place between short regions of homology (fro

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Parsons, R. L., P. V. Prasad, R. M. Harshey, and M. Jayaram. "Step-arrest mutants of FLP recombinase: implications for the catalytic mechanism of DNA recombination." Molecular and Cellular Biology 8, no. 8 (1988): 3303–10. http://dx.doi.org/10.1128/mcb.8.8.3303.

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The site-specific recombinase (FLP) encoded by the yeast plasmid 2 micron circle belongs to the integrase (of phage lambda) family of recombinases. The sparse homology within the members of this family contrasts with the invariance of three residues, His-396, Arg-399, and Tyr-433 (the numbers correspond to the family alignment positions), among them. We report here results on substrate recognition and catalysis by FLP proteins altered at these residues. Mutations of the conserved His and Tyr that aborted the reaction at specific steps of catalysis permitted genetic dissection of the possible b

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48

Parsons, R. L., P. V. Prasad, R. M. Harshey, and M. Jayaram. "Step-arrest mutants of FLP recombinase: implications for the catalytic mechanism of DNA recombination." Molecular and Cellular Biology 8, no. 8 (1988): 3303–10. http://dx.doi.org/10.1128/mcb.8.8.3303-3310.1988.

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The site-specific recombinase (FLP) encoded by the yeast plasmid 2 micron circle belongs to the integrase (of phage lambda) family of recombinases. The sparse homology within the members of this family contrasts with the invariance of three residues, His-396, Arg-399, and Tyr-433 (the numbers correspond to the family alignment positions), among them. We report here results on substrate recognition and catalysis by FLP proteins altered at these residues. Mutations of the conserved His and Tyr that aborted the reaction at specific steps of catalysis permitted genetic dissection of the possible b

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49

Shcherbakov, V. P., L. A. Plugina, and M. A. Nesheva. "Genetic recombination in bacteriophage T4: single-burst analysis of cosegregants and evidence in favor of a splice/patch coupling model." Genetics 131, no. 4 (1992): 769–81. http://dx.doi.org/10.1093/genetics/131.4.769.

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Abstract To reveal the structure of penultimate DNA intermediates in T4 bacteriophage recombination, resolution of which produces free recombinant molecules, a single-burst analysis of the recombinant progeny was made in multifactor crosses, enabling one to determine quantitatively the different recombinants generated by one or two exchanges within the same chromosome segment. It was found that double and single exchanges are highly correlated in T4 recombination. These results were interpreted as evidence for simultaneous formation of a splice/patch pair as the primary recombination products.

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Xiao, Hai-Long, Yong-Feng Jin, and Yao-Zhou Zhang. "Expression and Identification of Mutated Osteoprotegerin in Culture Cells and Larvae of Silkworm." Acta Biochimica et Biophysica Sinica 36, no. 5 (2004): 331–35. http://dx.doi.org/10.1093/abbs/36.5.331.

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Abstract The mutated osteoprotegerin (OPG-372) gene was inserted into the baculovirus transfer vector pBacPAK8, and the recombinant plasmid was co-transfected with linearized Bm-BacPAK6 virus DNA into BmN cells, then homologous recombination occurred inside the cells. The recombinant virus BmNPV-OPG-372 was screened and identified by Southern blotting. The recombinant human OPG-372 was expressed in cultured cells and the larvae of silkworm by inoculation of recombinant virus. The expression products were run on the SDS-PAGE and their immunoreactivities were determined by Western blotting. It w

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